Rh2AG398800

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0012833
Physical Location & Seq
Reverse (-)
59512427 .. 59513314
888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG398800.1

Sequence Viewer

Length: 681 bp
ATGGTCCTAAAAGAAACCATGCACAAAAGCAGGGTCTTCTATGACAAAACTCTTCAAAACCTCAAAAGCTTCTTCTGTGGAGGATACAAAAAACTACCCAAATCCCTTTCCTTGAATCCTTGCTTTTGTGGGAGCGGTGACCTGAAATATTACCCAAGAGATGAGTTTTACACTGATTATTATGATGAGTGGGAGTCTACTATAGATGAGGTGAAGAAGAGAGATAGTACATCAAAAGAAACAGTCAAGGATGAGGATCCATGTAGTGACAGCTTCATGATGTTTCCAAAGCAAAGTCCTAAGAAGAACAAACAAGAAGGGGGATTGTTAAGGGATAAGAAGTCTAGTGGAGGCTCTCGTGATCTTGGAAAAGGAAAAGACCAGTTATCATTCCAGAACATGAATGGTAATAGAGGGAGCAATGTTTTAGCGAAAAGGATGAAGGAATTTGAAATGGTGGATGCAGGTGATGTGGAGCAGGTGTTGGATGTAGAAGAGGCACTTCACTACTACTCTCGCCTCAAAAGTCCGGTGTACCTTGACATTGTTGACAAGTTTTTCTTAGATATGTACTCAGATTTCTCCGTCCCACCAGCACCATCGATCACCATCAACAATTCAAAGCAGAGAGTTGGCTCCCGGAGACTTGGCTCCCGAAGACTTGGGTCCATTAGGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD_2 Helicase_C_2

Protein Analysis

226

Amino Acids

25.97

Weight (kDa)

8.97

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012833)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16070
fragaria_vesca FvH4_6g31461
malus_domestica MD09G1210900.v1.1 MD17G1193700.v1.1
prunus_persica Prupe.3G069200_v2.0.a1
pyrus_communis pycom09g12850
rosa_chinensis RchiOBHm_Chr2g0138311
rosa_laevigata RLG00000019733
rosa_multiflora Rmu_sc0002475.1_g000007
rosa_roxburghii Rroxscaffold_2G00106340
rosa_rugosa Rorug02G0351100 Rorug02G0351200
rosa_samantha Rh2AG398800 Rh2BG408800 Rh2BG410200 Rh2CG385600 Rh2DG419100
rosa_wichuraiana Rw2G032500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 455, 469
Acc36I ACCTGC 2 cut(s) 455, 469
AccBSI CCGCTC 1 cut(s) 135
AccI GTMKAC 1 cut(s) 197
AciI CCGC 1 cut(s) 135
AclWI GGATC 2 cut(s) 251, 264
AcsI RAATTY 1 cut(s) 446
AfaI GTAC 3 cut(s) 229, 536, 572
AgsI TTSAA 4 cut(s) 56, 115, 452, 621
AluBI AGCT 2 cut(s) 69, 273
AluI AGCT 2 cut(s) 69, 273
Alw26I GTCTC 1 cut(s) 637
AlwI GGATC 2 cut(s) 251, 264
ApoI RAATTY 1 cut(s) 446
ArsI GACNNNNNNTTYG 2 cut(s) 18, 50
AspS9I GGNCC 2 cut(s) 4, 666
AsuC2I CCSGG 1 cut(s) 640
AsuHPI GGTGA 4 cut(s) 149, 223, 479, 598
AvaII GGWCC 2 cut(s) 4, 666
BamHI GGATCC 1 cut(s) 256
BauI CACGAG 1 cut(s) 357
BbsI GAAGAC 2 cut(s) 28, 664
BccI CCATC 2 cut(s) 607, 617
BciVI GTATCC 1 cut(s) 77
BcnI CCSGG 1 cut(s) 640
BcoDI GTCTC 1 cut(s) 637
BfaI CTAG 1 cut(s) 345
BfmI CTRYAG 1 cut(s) 201
BfuAI ACCTGC 2 cut(s) 455, 469
BfuI GTATCC 1 cut(s) 77
Bme1390I CCNGG 1 cut(s) 640
Bme18I GGWCC 2 cut(s) 4, 666
BmgT120I GGNCC 2 cut(s) 4, 666
BmiI GGNNCC 4 cut(s) 258, 637, 652, 667
BmrFI CCNGG 1 cut(s) 640
BmsI GCATC 1 cut(s) 451
BoxI GACNNNNGTC 1 cut(s) 664
BpiI GAAGAC 2 cut(s) 28, 664
BpuMI CCSGG 1 cut(s) 640
Bsa29I ATCGAT 1 cut(s) 602
BsaBI GATNNNNATC 2 cut(s) 255, 608
BsaWI WCCGGW 1 cut(s) 529
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bse1I ACTGG 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 427
Bse8I GATNNNNATC 2 cut(s) 255, 608
BseCI ATCGAT 1 cut(s) 602
BseGI GGATG 4 cut(s) 256, 444, 466, 493
BseJI GATNNNNATC 2 cut(s) 255, 608
BseMI GCAATG 1 cut(s) 427
BseMII CTCAG 1 cut(s) 588
BseNI ACTGG 1 cut(s) 382
BshVI ATCGAT 1 cut(s) 602
BsiSI CCGG 2 cut(s) 530, 640
BslFI GGGAC 1 cut(s) 572
BsmAI GTCTC 1 cut(s) 637
BsmFI GGGAC 1 cut(s) 572
Bsp143I GATC 3 cut(s) 256, 361, 603
BspACI CCGC 1 cut(s) 135
BspCNI CTCAG 1 cut(s) 587
BspDI ATCGAT 1 cut(s) 602
BspHI TCATGA 1 cut(s) 276
BspLI GGNNCC 4 cut(s) 258, 637, 652, 667
BspMI ACCTGC 2 cut(s) 455, 469
BspPI GGATC 2 cut(s) 251, 264
BsrBI CCGCTC 1 cut(s) 135
BsrDI GCAATG 1 cut(s) 427
BsrI ACTGG 1 cut(s) 382
BssMI GATC 3 cut(s) 256, 361, 603
BssSI CACGAG 1 cut(s) 357
Bst2BI CACGAG 1 cut(s) 357
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 3 cut(s) 57, 212, 489
BstDEI CTNAG 3 cut(s) 300, 562, 574
BstEII GGTNACC 1 cut(s) 137
BstF5I GGATG 4 cut(s) 256, 444, 466, 493
BstKTI GATC 3 cut(s) 259, 364, 606
BstMAI GTCTC 1 cut(s) 637
BstMBI GATC 3 cut(s) 256, 361, 603
BstPAI GACNNNNGTC 1 cut(s) 664
BstPI GGTNACC 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 638
BstSFI CTRYAG 1 cut(s) 201
BstV2I GAAGAC 2 cut(s) 28, 664
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
Bsu15I ATCGAT 1 cut(s) 602
BsuI GTATCC 1 cut(s) 77
BsuTUI ATCGAT 1 cut(s) 602
BtsCI GGATG 4 cut(s) 256, 444, 466, 493
BtsIMutI CAGTG 1 cut(s) 171
BveI ACCTGC 2 cut(s) 455, 469
CciI TCATGA 1 cut(s) 276
Cfr13I GGNCC 2 cut(s) 4, 666
ClaI ATCGAT 1 cut(s) 602
Csp6I GTAC 3 cut(s) 228, 535, 571
CviAII CATG 4 cut(s) 19, 261, 277, 400
CviJI RGCY 5 cut(s) 69, 273, 354, 636, 651
CviKI_1 RGCY 5 cut(s) 69, 273, 354, 636, 651
CviQI GTAC 3 cut(s) 228, 535, 571
DdeI CTNAG 3 cut(s) 300, 562, 574
DpnI GATC 3 cut(s) 258, 363, 605
DpnII GATC 3 cut(s) 256, 361, 603
Eam1104I CTCTTC 3 cut(s) 57, 212, 489
EarI CTCTTC 3 cut(s) 57, 212, 489
Eco47I GGWCC 2 cut(s) 4, 666
Eco91I GGTNACC 1 cut(s) 137
EcoO65I GGTNACC 1 cut(s) 137
FaeI CATG 4 cut(s) 22, 264, 280, 403
FaiI YATR 8 cut(s) 20, 42, 183, 203, 262, 278, 401, 569
FalI AAGNNNNNCTT 4 cut(s) 486, 518, 545, 577
FaqI GGGAC 1 cut(s) 572
FatI CATG 4 cut(s) 18, 260, 276, 399
FblI GTMKAC 1 cut(s) 197
FokI GGATG 4 cut(s) 263, 451, 473, 500
FspBI CTAG 1 cut(s) 345
HapII CCGG 2 cut(s) 530, 640
Hin1II CATG 4 cut(s) 22, 264, 280, 403
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 1 cut(s) 67
HinfI GANTC 2 cut(s) 115, 194
HpaII CCGG 2 cut(s) 530, 640
HphI GGTGA 4 cut(s) 149, 223, 479, 598
Hpy166II GTNNAC 3 cut(s) 198, 535, 550
Hpy188I TCNGA 1 cut(s) 577
Hpy188III TCNNGA 4 cut(s) 277, 359, 394, 654
Hpy8I GTNNAC 3 cut(s) 198, 535, 550
HpyAV CCTTC 2 cut(s) 311, 436
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 2 cut(s) 22, 464
HpyF3I CTNAG 3 cut(s) 300, 562, 574
Hsp92II CATG 4 cut(s) 22, 264, 280, 403
Kzo9I GATC 3 cut(s) 256, 361, 603
LmnI GCTCC 5 cut(s) 132, 417, 475, 641, 656
LpnPI CCDG 9 cut(s) 16, 155, 395, 407, 450, 464, 543, 606, 653
LweI GCATC 1 cut(s) 451
MaeI CTAG 1 cut(s) 345
MaeIII GTNAC 2 cut(s) 137, 266
MalI GATC 3 cut(s) 258, 363, 605
MbiI CCGCTC 1 cut(s) 135
MboI GATC 3 cut(s) 256, 361, 603
MboII GAAGA 8 cut(s) 28, 44, 64, 226, 229, 316, 506, 669
MflI RGATCY 1 cut(s) 256
MluCI AATT 2 cut(s) 446, 616
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 465
MnlI CCTC 8 cut(s) 71, 74, 202, 247, 344, 407, 490, 530
MseI TTAA 1 cut(s) 329
MspI CCGG 2 cut(s) 530, 640
MspR9I CCNGG 1 cut(s) 640
NciI CCSGG 1 cut(s) 640
NdeII GATC 3 cut(s) 256, 361, 603
NlaIII CATG 4 cut(s) 22, 264, 280, 403
NlaIV GGNNCC 4 cut(s) 258, 637, 652, 667
NmuCI GTSAC 2 cut(s) 137, 266
PagI TCATGA 1 cut(s) 276
PaqCI CACCTGC 2 cut(s) 455, 469
PfeI GAWTC 1 cut(s) 115
PfoI TCCNGGA 1 cut(s) 638
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PshAI GACNNNNGTC 1 cut(s) 664
PspEI GGTNACC 1 cut(s) 137
PspN4I GGNNCC 4 cut(s) 258, 637, 652, 667
PspPI GGNCC 2 cut(s) 4, 666
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 3 cut(s) 229, 536, 572
RsaNI GTAC 3 cut(s) 228, 535, 571
SaqAI TTAA 1 cut(s) 329
Sau3AI GATC 3 cut(s) 256, 361, 603
Sau96I GGNCC 2 cut(s) 4, 666
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 640
SetI ASST 9 cut(s) 63, 71, 144, 213, 275, 469, 483, 540, 677
SfaNI GCATC 1 cut(s) 451
SfcI CTRYAG 1 cut(s) 201
SinI GGWCC 2 cut(s) 4, 666
Sse9I AATT 2 cut(s) 446, 616
SsiI CCGC 1 cut(s) 135
SspI AATATT 1 cut(s) 149
SspMI CTAG 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 638
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 1 cut(s) 602
TasI AATT 2 cut(s) 446, 616
TatI WGTACW 2 cut(s) 227, 570
TfiI GAWTC 1 cut(s) 115
Tru1I TTAA 1 cut(s) 329
Tru9I TTAA 1 cut(s) 329
TscAI CASTG 1 cut(s) 178
TseFI GTSAC 2 cut(s) 137, 266
Tsp45I GTSAC 2 cut(s) 137, 266
TspDTI ATGAA 3 cut(s) 265, 416, 455
TspGWI ACGGA 1 cut(s) 574
TspRI CASTG 1 cut(s) 178
VpaK11BI GGWCC 2 cut(s) 4, 666
XapI RAATTY 1 cut(s) 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 401
XmiI GTMKAC 1 cut(s) 197
XspI CTAG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.