RLG00000019733

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012833
Physical Location & Seq
Reverse (-)
56101549 .. 56102208
660 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019733

Sequence Viewer

Length: 660 bp
ATGCACAAAAGCAAGCTCTTCTATGATAAAACTCTTCAAAACCTCAAAAGCTTCTTCTGTGGAGGATACAAAAAACTACCCAAATCCCTTTCCTTGAATCCTTGCTTTTGTGTGAGTGACCTGAAATATCACCCAACAGATGATTTTTACACTGATTATTATGATGAGTGGGAGTCTACTATAGATGAGGTGAAGAAGAGAGATAGTACATCAAAAGAAACAATCAAGGATGAGGATCCATGTAGTGAAAGCTTCATGATGTTTCCAAAGCAAAGTCCTAAGAAGAACAAACAAGAAGGGGGATTGTTAAGGGATAAGAAGTCTAGTGGAGGCTCTCGTGATCTTGGAAAAGGAAAAGACCAGTTATCATTCCAGAACATGAATGGTAATAGAGGGAGCAATGTTTTAGCGAGAAGGATGAAGGAGTTTGAAATGGTGGATGCATGTGATGTGGAGCAGGTGTTGGATGTAGAAGAGGCACTTCACTACTACTCTCGCCTCAAAAGTCCCGTGTACCTTGACATTGTTGACAAGTTTTTCTTAGATATGTACTCAGATTTCTCCGTCCCACCAGCACCATCGATCAGCATCAACAATTCAAAGCAGAGAGTTGGCTCCCGGAGACTTGGCTCCCGAAGACTTGGCTCCATTAGGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD_2 Helicase_C_2

Protein Analysis

220

Amino Acids

25.23

Weight (kDa)

8.81

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012833)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16070
fragaria_vesca FvH4_6g31461
malus_domestica MD09G1210900.v1.1 MD17G1193700.v1.1
prunus_persica Prupe.3G069200_v2.0.a1
pyrus_communis pycom09g12850
rosa_chinensis RchiOBHm_Chr2g0138311
rosa_laevigata RLG00000019733
rosa_multiflora Rmu_sc0002475.1_g000007
rosa_roxburghii Rroxscaffold_2G00106340
rosa_rugosa Rorug02G0351100 Rorug02G0351200
rosa_samantha Rh2AG398800 Rh2BG408800 Rh2BG410200 Rh2CG385600 Rh2DG419100
rosa_wichuraiana Rw2G032500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 448
Acc36I ACCTGC 1 cut(s) 448
AccI GTMKAC 1 cut(s) 176
AclWI GGATC 2 cut(s) 230, 243
AfaI GTAC 3 cut(s) 208, 515, 551
AgsI TTSAA 4 cut(s) 38, 97, 431, 600
AluBI AGCT 3 cut(s) 16, 51, 252
AluI AGCT 3 cut(s) 16, 51, 252
Alw26I GTCTC 1 cut(s) 616
AlwI GGATC 2 cut(s) 230, 243
AsuC2I CCSGG 1 cut(s) 619
AsuHPI GGTGA 2 cut(s) 122, 202
BamHI GGATCC 1 cut(s) 235
BauI CACGAG 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 643
BccI CCATC 1 cut(s) 586
BciVI GTATCC 1 cut(s) 59
BcnI CCSGG 1 cut(s) 619
BcoDI GTCTC 1 cut(s) 616
BfaI CTAG 1 cut(s) 324
BfmI CTRYAG 1 cut(s) 180
BfuAI ACCTGC 1 cut(s) 448
BfuI GTATCC 1 cut(s) 59
Bme1390I CCNGG 1 cut(s) 619
BmiI GGNNCC 4 cut(s) 237, 616, 631, 646
BmrFI CCNGG 1 cut(s) 619
BmsI GCATC 2 cut(s) 430, 597
BpiI GAAGAC 1 cut(s) 643
BpuMI CCSGG 1 cut(s) 619
Bsa29I ATCGAT 1 cut(s) 581
BsaBI GATNNNNATC 2 cut(s) 234, 587
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 1 cut(s) 406
Bse8I GATNNNNATC 2 cut(s) 234, 587
BseCI ATCGAT 1 cut(s) 581
BseGI GGATG 4 cut(s) 235, 423, 445, 472
BseJI GATNNNNATC 2 cut(s) 234, 587
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 1 cut(s) 567
BseNI ACTGG 1 cut(s) 361
BshVI ATCGAT 1 cut(s) 581
BsiSI CCGG 1 cut(s) 619
BslFI GGGAC 2 cut(s) 492, 551
BsmAI GTCTC 1 cut(s) 616
BsmFI GGGAC 2 cut(s) 492, 551
Bsp143I GATC 3 cut(s) 235, 340, 582
BspCNI CTCAG 1 cut(s) 566
BspDI ATCGAT 1 cut(s) 581
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 4 cut(s) 237, 616, 631, 646
BspMI ACCTGC 1 cut(s) 448
BspPI GGATC 2 cut(s) 230, 243
BspQI GCTCTTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 406
BsrI ACTGG 1 cut(s) 361
BssMI GATC 3 cut(s) 235, 340, 582
BssSI CACGAG 1 cut(s) 336
Bst2BI CACGAG 1 cut(s) 336
Bst6I CTCTTC 4 cut(s) 23, 39, 191, 468
BstC8I GCNNGC 1 cut(s) 14
BstDEI CTNAG 3 cut(s) 279, 541, 553
BstF5I GGATG 4 cut(s) 235, 423, 445, 472
BstKTI GATC 3 cut(s) 238, 343, 585
BstMAI GTCTC 1 cut(s) 616
BstMBI GATC 3 cut(s) 235, 340, 582
BstNSI RCATGY 1 cut(s) 447
BstSCI CCNGG 1 cut(s) 617
BstSFI CTRYAG 1 cut(s) 180
BstV2I GAAGAC 1 cut(s) 643
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
Bsu15I ATCGAT 1 cut(s) 581
BsuI GTATCC 1 cut(s) 59
BsuTUI ATCGAT 1 cut(s) 581
BtsCI GGATG 4 cut(s) 235, 423, 445, 472
BtsIMutI CAGTG 1 cut(s) 150
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 14
CciI TCATGA 1 cut(s) 255
ClaI ATCGAT 1 cut(s) 581
Csp6I GTAC 3 cut(s) 207, 514, 550
CviAII CATG 4 cut(s) 240, 256, 379, 444
CviJI RGCY 7 cut(s) 16, 51, 252, 333, 615, 630, 645
CviKI_1 RGCY 7 cut(s) 16, 51, 252, 333, 615, 630, 645
CviQI GTAC 3 cut(s) 207, 514, 550
DdeI CTNAG 3 cut(s) 279, 541, 553
DpnI GATC 3 cut(s) 237, 342, 584
DpnII GATC 3 cut(s) 235, 340, 582
Eam1104I CTCTTC 4 cut(s) 23, 39, 191, 468
EarI CTCTTC 4 cut(s) 23, 39, 191, 468
EcoT22I ATGCAT 1 cut(s) 445
FaeI CATG 4 cut(s) 243, 259, 382, 447
FaiI YATR 8 cut(s) 24, 162, 182, 241, 257, 380, 445, 548
FalI AAGNNNNNCTT 4 cut(s) 465, 497, 524, 556
FaqI GGGAC 2 cut(s) 492, 551
FatI CATG 4 cut(s) 239, 255, 378, 443
FblI GTMKAC 1 cut(s) 176
FokI GGATG 4 cut(s) 242, 430, 452, 479
FspBI CTAG 1 cut(s) 324
HapII CCGG 1 cut(s) 619
Hin1II CATG 4 cut(s) 243, 259, 382, 447
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HindIII AAGCTT 2 cut(s) 49, 250
HinfI GANTC 2 cut(s) 97, 173
HpaII CCGG 1 cut(s) 619
HphI GGTGA 2 cut(s) 122, 202
Hpy166II GTNNAC 3 cut(s) 177, 514, 529
Hpy188I TCNGA 1 cut(s) 556
Hpy188III TCNNGA 4 cut(s) 256, 338, 373, 633
Hpy8I GTNNAC 3 cut(s) 177, 514, 529
HpyAV CCTTC 3 cut(s) 290, 408, 415
HpyCH4V TGCA 2 cut(s) 4, 443
HpyF3I CTNAG 3 cut(s) 279, 541, 553
Hsp92II CATG 4 cut(s) 243, 259, 382, 447
Kzo9I GATC 3 cut(s) 235, 340, 582
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 5 cut(s) 396, 454, 620, 635, 650
LpnPI CCDG 6 cut(s) 134, 374, 386, 443, 585, 632
LweI GCATC 2 cut(s) 430, 597
MaeI CTAG 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 116
MalI GATC 3 cut(s) 237, 342, 584
MboI GATC 3 cut(s) 235, 340, 582
MboII GAAGA 8 cut(s) 10, 26, 46, 205, 208, 295, 485, 648
MflI RGATCY 1 cut(s) 235
MluCI AATT 1 cut(s) 595
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 444
MnlI CCTC 8 cut(s) 53, 56, 181, 226, 323, 386, 469, 509
Mph1103I ATGCAT 1 cut(s) 445
MseI TTAA 1 cut(s) 308
MspI CCGG 1 cut(s) 619
MspR9I CCNGG 1 cut(s) 619
NciI CCSGG 1 cut(s) 619
NdeII GATC 3 cut(s) 235, 340, 582
NlaIII CATG 4 cut(s) 243, 259, 382, 447
NlaIV GGNNCC 4 cut(s) 237, 616, 631, 646
NmuCI GTSAC 1 cut(s) 116
NsiI ATGCAT 1 cut(s) 445
NspI RCATGY 1 cut(s) 447
PagI TCATGA 1 cut(s) 255
PaqCI CACCTGC 1 cut(s) 448
PciSI GCTCTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 97
PfoI TCCNGGA 1 cut(s) 617
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PspN4I GGNNCC 4 cut(s) 237, 616, 631, 646
PsuI RGATCY 1 cut(s) 235
RsaI GTAC 3 cut(s) 208, 515, 551
RsaNI GTAC 3 cut(s) 207, 514, 550
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 1 cut(s) 308
Sau3AI GATC 3 cut(s) 235, 340, 582
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 1 cut(s) 619
SetI ASST 9 cut(s) 18, 45, 53, 123, 192, 254, 462, 519, 656
SfaNI GCATC 2 cut(s) 430, 597
SfcI CTRYAG 1 cut(s) 180
Sse9I AATT 1 cut(s) 595
SspMI CTAG 1 cut(s) 324
StyD4I CCNGG 1 cut(s) 617
TaqI TCGA 1 cut(s) 581
TasI AATT 1 cut(s) 595
TatI WGTACW 2 cut(s) 206, 549
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 244, 395, 434
TspGWI ACGGA 1 cut(s) 553
TspRI CASTG 1 cut(s) 157
XceI RCATGY 1 cut(s) 447
XcmI CCANNNNNNNNNTGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 176
XspI CTAG 1 cut(s) 324
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.