pycom09g14530

Chaperone protein dnaJ 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0013509
Physical Location & Seq
Reverse (-)
14181057 .. 14184580
3524 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 852 bp
ATGATATTTTTTTTGTACTTTACCCAAAAAACTTTTTTTTTCCTTCCTCCTTTTTTTCCTCTTCTTCTTCTTCTTCCTCCTCCTTCTCCTTCTTCCTCTTCCTTTTCTTCCTCTTCTTCTTCTTCCTCCTTCTCCTTCTCCCTTCTTCTTCTTCCGCGCCGATGGCCGCGACCGCAAGGGGAATTTTTTTTTTTTCCCCTTCCTCCTTGTTTTCCTCTTATTCTTCTTCTTCTTCCTTCTCCTCCTCCTTCTCCTTCTTGTTCTTCTACAGGGGGTTGTTGGGTTTTCAAATTTTTTTTTTTTTTTTTTGGGTTTTGGGTTTTTTTTTTTTTTTTTTTTTTGAATAGCCAGAGGATTATTGGGTTTTCTGAGATTTTGGGAATGGCGTGTGTTGGAGCAGGTGTGATTGGGGGCAATGGGTCGTCCTGGAATCTGTTCAAGGACAAGGCAAGGAAGAAGAAGATGGTGAATAATAATAAGAGGGTGATGGTTTCTTGTGTTGCTTCTTCCGTGATCGATCCTTACCGGACTTTGAAGATTCAACCCGGTGCCTCTGAATCTGAGGTCAGGAAGGCGTTTAGACAACTTGCCTTGAAGTATCATCCAGACGTATGCAGAGGAAACAATTGTGGAGTTGAGTTTCACCAGATCAATGAAGCCTATGATATTGTTATGAGCAATTTGAGAGGAGAAACGACGACTCCATTGCAGATGTATGAGACGTACGATGAAGGGGATGAAGAGCCGATGAGGGGAATGAACGATCCGGATTGGGACATGTGGGAGGAATGGATGGGATGGGAAGGAGCTGGAATTAGAGACTACACATCTCACATTAATCCTTATATTTGA

Protein Analysis

284

Amino Acids

32.43

Weight (kDa)

6.82

Isoelectric Point (pI)

59.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 173 - 225 1.9e-14 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80920
fragaria_vesca FvH4_6g28480
malus_domestica MD09G1225800.v1.1 MD17G1224600.v1.1
prunus_persica Prupe.3G100300_v2.0.a1
pyrus_communis pycom09g14530 pycom17g22830
rosa_chinensis RchiOBHm_Chr2g0132701
rosa_laevigata RLG00000019273
rosa_multiflora Rmu_ssc0000074.1_g000011
rosa_roxburghii Rroxscaffold_2G00111720
rosa_rugosa Rorug02G0306300
rosa_samantha Rh2AG359400 Rh2BG365700 Rh2CG342500 Rh2DG382400
rosa_wichuraiana Rw2G029240 Rw3G024410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 389
AccB1I GGYRCC 1 cut(s) 548
AccII CGCG 2 cut(s) 157, 169
AccIII TCCGGA 1 cut(s) 766
AciI CCGC 3 cut(s) 155, 167, 173
AclWI GGATC 2 cut(s) 512, 758
AcoI YGGCCR 1 cut(s) 164
AcsI RAATTY 2 cut(s) 182, 290
AfaI GTAC 2 cut(s) 17, 725
AfiI CCNNNNNNNGG 1 cut(s) 752
AflIII ACRYGT 1 cut(s) 777
AgsI TTSAA 6 cut(s) 289, 343, 439, 535, 542, 595
AjnI CCWGG 1 cut(s) 425
AluBI AGCT 1 cut(s) 809
AluI AGCT 1 cut(s) 809
Alw26I GTCTC 2 cut(s) 713, 813
AlwI GGATC 2 cut(s) 512, 758
Aor13HI TCCGGA 1 cut(s) 766
AoxI GGCC 1 cut(s) 164
ApoI RAATTY 2 cut(s) 182, 290
AseI ATTAAT 1 cut(s) 837
Asp700I GAANNNNTTC 1 cut(s) 434
AspLEI GCGC 1 cut(s) 159
AsuC2I CCSGG 1 cut(s) 546
AsuHPI GGTGA 3 cut(s) 478, 496, 635
BanI GGYRCC 1 cut(s) 548
BccI CCATC 5 cut(s) 156, 457, 481, 787, 792
BcgI CGANNNNNNTGC 2 cut(s) 688, 722
BciT130I CCWGG 1 cut(s) 427
BcnI CCSGG 1 cut(s) 546
BcoDI GTCTC 2 cut(s) 713, 813
BfmI CTRYAG 1 cut(s) 267
BfuAI ACCTGC 1 cut(s) 389
BisI GCNGC 1 cut(s) 167
BlsI GCNGC 1 cut(s) 168
Bme1390I CCNGG 2 cut(s) 427, 546
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 2 cut(s) 427, 546
BpuMI CCSGG 1 cut(s) 546
Bsa29I ATCGAT 1 cut(s) 516
BsaWI WCCGGW 2 cut(s) 525, 766
BsaXI ACNNNNNCTCC 4 cut(s) 387, 417, 685, 715
Bsc4I CCNNNNNNNGG 1 cut(s) 752
Bse3DI GCAATG 2 cut(s) 421, 704
BseAI TCCGGA 1 cut(s) 766
BseBI CCWGG 1 cut(s) 427
BseCI ATCGAT 1 cut(s) 516
BseGI GGATG 4 cut(s) 601, 742, 798, 803
BseLI CCNNNNNNNGG 1 cut(s) 752
BseMI GCAATG 2 cut(s) 421, 704
BseMII CTCAG 2 cut(s) 360, 552
BseRI GAGGAG 4 cut(s) 69, 231, 234, 702
Bsh1236I CGCG 2 cut(s) 157, 169
Bsh1285I CGRYCG 1 cut(s) 173
BshFI GGCC 1 cut(s) 166
BshNI GGYRCC 1 cut(s) 548
BshVI ATCGAT 1 cut(s) 516
BsiEI CGRYCG 1 cut(s) 173
BsiSI CCGG 3 cut(s) 526, 546, 767
BsiWI CGTACG 1 cut(s) 723
BslFI GGGAC 1 cut(s) 788
BslI CCNNNNNNNGG 1 cut(s) 752
BsmAI GTCTC 2 cut(s) 713, 813
BsmBI CGTCTC 1 cut(s) 713
BsmFI GGGAC 1 cut(s) 788
BsnI GGCC 1 cut(s) 166
Bsp13I TCCGGA 1 cut(s) 766
Bsp143I GATC 4 cut(s) 513, 517, 648, 763
BspACI CCGC 3 cut(s) 155, 167, 173
BspANI GGCC 1 cut(s) 166
BspCNI CTCAG 2 cut(s) 361, 553
BspDI ATCGAT 1 cut(s) 516
BspEI TCCGGA 1 cut(s) 766
BspFNI CGCG 2 cut(s) 157, 169
BspLI GGNNCC 1 cut(s) 550
BspMI ACCTGC 1 cut(s) 389
BspPI GGATC 2 cut(s) 512, 758
BspQI GCTCTTC 1 cut(s) 735
BspT107I GGYRCC 1 cut(s) 548
BsrDI GCAATG 2 cut(s) 421, 704
BssMI GATC 4 cut(s) 513, 517, 648, 763
Bst2UI CCWGG 1 cut(s) 427
Bst6I CTCTTC 4 cut(s) 66, 103, 118, 735
BstDEI CTNAG 2 cut(s) 369, 561
BstF5I GGATG 4 cut(s) 601, 742, 798, 803
BstFNI CGCG 2 cut(s) 157, 169
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 4 cut(s) 516, 520, 651, 766
BstMAI GTCTC 2 cut(s) 713, 813
BstMBI GATC 4 cut(s) 513, 517, 648, 763
BstMCI CGRYCG 1 cut(s) 173
BstMWI GCNNNNNNNGC 2 cut(s) 163, 172
BstNI CCWGG 1 cut(s) 427
BstNSI RCATGY 1 cut(s) 781
BstSCI CCNGG 2 cut(s) 425, 544
BstSFI CTRYAG 1 cut(s) 267
BstUI CGCG 2 cut(s) 157, 169
Bsu15I ATCGAT 1 cut(s) 516
BsuRI GGCC 1 cut(s) 166
BsuTUI ATCGAT 1 cut(s) 516
BtsCI GGATG 4 cut(s) 601, 742, 798, 803
BveI ACCTGC 1 cut(s) 389
CfoI GCGC 1 cut(s) 159
ClaI ATCGAT 1 cut(s) 516
Csp6I GTAC 2 cut(s) 16, 724
CviAII CATG 1 cut(s) 778
CviJI RGCY 5 cut(s) 166, 348, 659, 745, 809
CviKI_1 RGCY 5 cut(s) 166, 348, 659, 745, 809
CviQI GTAC 2 cut(s) 16, 724
DdeI CTNAG 2 cut(s) 369, 561
DpnI GATC 4 cut(s) 515, 519, 650, 765
DpnII GATC 4 cut(s) 513, 517, 648, 763
EaeI YGGCCR 1 cut(s) 164
Eam1104I CTCTTC 4 cut(s) 66, 103, 118, 735
EarI CTCTTC 4 cut(s) 66, 103, 118, 735
EcoRII CCWGG 1 cut(s) 425
Esp3I CGTCTC 1 cut(s) 713
FaeI CATG 1 cut(s) 781
FaiI YATR 6 cut(s) 613, 663, 674, 717, 779, 846
FaqI GGGAC 1 cut(s) 788
FatI CATG 1 cut(s) 777
Fnu4HI GCNGC 1 cut(s) 167
FokI GGATG 4 cut(s) 588, 749, 805, 810
Fsp4HI GCNGC 1 cut(s) 167
GlaI GCGC 1 cut(s) 158
GluI GCNGC 1 cut(s) 167
HaeIII GGCC 1 cut(s) 166
HapII CCGG 3 cut(s) 526, 546, 767
HhaI GCGC 1 cut(s) 159
Hin1II CATG 1 cut(s) 781
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HinfI GANTC 4 cut(s) 430, 538, 557, 700
HpaII CCGG 3 cut(s) 526, 546, 767
HphI GGTGA 3 cut(s) 478, 496, 635
Hpy188I TCNGA 3 cut(s) 370, 556, 562
Hpy188III TCNNGA 3 cut(s) 568, 605, 767
Hpy99I CGWCG 1 cut(s) 700
HpyCH4IV ACGT 2 cut(s) 609, 722
HpyCH4V TGCA 2 cut(s) 615, 709
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 172
HpyF3I CTNAG 2 cut(s) 369, 561
HpySE526I ACGT 2 cut(s) 609, 722
Hsp92II CATG 1 cut(s) 781
HspAI GCGC 1 cut(s) 157
Kpn2I TCCGGA 1 cut(s) 766
Kzo9I GATC 4 cut(s) 513, 517, 648, 763
LguI GCTCTTC 1 cut(s) 735
LmnI GCTCC 2 cut(s) 395, 806
MaeII ACGT 2 cut(s) 609, 722
MalI GATC 4 cut(s) 515, 519, 650, 765
MboI GATC 4 cut(s) 513, 517, 648, 763
MfeI CAATTG 1 cut(s) 625
MluCI AATT 5 cut(s) 182, 290, 625, 679, 813
MlyI GAGTC 1 cut(s) 694
MmeI TCCRAC 1 cut(s) 373
MroI TCCGGA 1 cut(s) 766
MroXI GAANNNNTTC 1 cut(s) 434
MseI TTAA 1 cut(s) 837
MspI CCGG 3 cut(s) 526, 546, 767
MspR9I CCNGG 2 cut(s) 427, 546
MunI CAATTG 1 cut(s) 625
MvaI CCWGG 1 cut(s) 427
MvnI CGCG 2 cut(s) 157, 169
MwoI GCNNNNNNNGC 2 cut(s) 163, 172
NciI CCSGG 1 cut(s) 546
NdeII GATC 4 cut(s) 513, 517, 648, 763
NlaIII CATG 1 cut(s) 781
NlaIV GGNNCC 1 cut(s) 550
NspI RCATGY 1 cut(s) 781
PaqCI CACCTGC 1 cut(s) 389
PciI ACATGT 1 cut(s) 777
PciSI GCTCTTC 1 cut(s) 735
PdmI GAANNNNTTC 1 cut(s) 434
PfeI GAWTC 3 cut(s) 430, 538, 557
Pfl23II CGTACG 1 cut(s) 723
PfoI TCCNGGA 1 cut(s) 425
PkrI GCNGC 1 cut(s) 168
PleI GAGTC 1 cut(s) 694
PpsI GAGTC 1 cut(s) 694
PscI ACATGT 1 cut(s) 777
PshBI ATTAAT 1 cut(s) 837
Psp6I CCWGG 1 cut(s) 425
PspGI CCWGG 1 cut(s) 425
PspLI CGTACG 1 cut(s) 723
PspN4I GGNNCC 1 cut(s) 550
RsaI GTAC 2 cut(s) 17, 725
RsaNI GTAC 2 cut(s) 16, 724
SapI GCTCTTC 1 cut(s) 735
SaqAI TTAA 1 cut(s) 837
SatI GCNGC 1 cut(s) 167
Sau3AI GATC 4 cut(s) 513, 517, 648, 763
SchI GAGTC 1 cut(s) 694
ScrFI CCNGG 2 cut(s) 427, 546
SetI ASST 5 cut(s) 403, 567, 612, 725, 811
SfcI CTRYAG 1 cut(s) 267
Sse9I AATT 5 cut(s) 182, 290, 625, 679, 813
SsiI CCGC 3 cut(s) 155, 167, 173
StyD4I CCNGG 2 cut(s) 425, 544
TaiI ACGT 2 cut(s) 612, 725
TaqI TCGA 1 cut(s) 516
TasI AATT 5 cut(s) 182, 290, 625, 679, 813
TatI WGTACW 1 cut(s) 15
TauI GCSGC 1 cut(s) 169
TfiI GAWTC 3 cut(s) 430, 538, 557
Tru1I TTAA 1 cut(s) 837
Tru9I TTAA 1 cut(s) 837
TspDTI ATGAA 4 cut(s) 669, 744, 753, 773
TspGWI ACGGA 1 cut(s) 499
VspI ATTAAT 1 cut(s) 837
XapI RAATTY 2 cut(s) 182, 290
XceI RCATGY 1 cut(s) 781
XcmI CCANNNNNNNNNTGG 1 cut(s) 356
XmnI GAANNNNTTC 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.