pycom09g14750

Belongs to the TUB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007280
Physical Location & Seq
Reverse (-)
14503831 .. 14506391
2561 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1356 bp
ATGATGATGGATGGTTGCAAGAAAACCACACTAATTCCACGGCAATCCTCATACAACTCTCTCTATGACAATCCCCTCACAGACCAGAAACACAGCCGCAGCTACAGCGAAGGCATCGCTTTCACCTCCAAGAACGACAGCTCGAACCCGAACCAATCACCCTTAAACGACAACAAGGAGAACGCAATGCCCAACAAGCCACAGCAGGCCGTGGCAGACAGCGACAAGGAGAATTTGGTCGCACAAGAAAACGGGTCTTCCTCTTTTGTGATGCAGCCATTGAAGCCGAGGAATTTGAAGTCTCTGTCCACTGGCTGCAGGGCCCTCAAGCCCTCCTCGCTTCAGTTCTGTATGCAGGCGAATGAGCCTGAGAAGGCACTCTTTGGTGGGTCCATATCCACAATGTGGGACCCACCCAGCAATGGGTCCGACAACTCGAGCTCTTTGAAGATCTGGGACTACTCCGATTCTGAGGCTGCCCCGGCCTCATCTTGGTCTAGTCTGCCTAACAAGTCATTGCTTTGCAGGCCATTGCCAATGGATGTGGGAAGGTGCACATGTGTTATAGTGAAGGAAGCAACCCCAGAAGGATTAGATGGGGGCACTCTCTATTCTCTTTATACCAATGAAGGCAAGGGAAGGCAGGACCGGAAACTAGCTATTGCTCACCATAAGCGGCGGAACGGGAAATCTGAGTTAACTATTGCTCAGAATGTGAAGGGAGTAATGTCTCATTCAGATGATAGTTTTATTGGGATTGTAAATGCTAACCTCATGGGTTCAAAATACCACATATGGGATCAGGGTGGTCGTCCCAATTCCTTGAGCAAGGAGGCTAAATCACTTGCTGTTGTAGCGTTCACGCCAACAATCTCGAGCTGGACAGGAAGTTACAGACGTATGAGGGCATATATACCCAAGCACCAATCCATGCAGTTAAAAAACACAACGCAGGTGATCTTAACAGTACAACACATTAAGGGACTGCCAAAGGACTGGGAAGGGAAGCTTGACAAAGTTCATCAGCTATTCTCACGAGCTCCCCATTACAATAAAATTTCAAAGCAATATGAGTTAGACTTTAGAGATAGGGGAAGAGCGGGTCTTAGAATCCAAAGTTCGGTCAAGAATTTCCAACTGACACTGGAGGTAACTACTATGAACGATCGTGATTCCGAAAATCTTACTCAGGCTTATCTGAAACACGTATATGAAAACGGGAAGCAGACAATTCTGCAGCTCGGAAGGGTCGGGAAATCGAAGTATGTGATGGATTACAGATATCCTTTGACAGGCTACCAAGCGTTTTGCATCTGTTTGGCTTCTATTGATTCAAAACTTTGCTGCACGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0035091 GO:0043167 GO:0043168
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tub

Protein Analysis

452

Amino Acids

50.32

Weight (kDa)

9.28

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 174 - 445 4.4e-46 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 943
Acc36I ACCTGC 1 cut(s) 943
AccB7I CCANNNNNTGG 1 cut(s) 405
AccBSI CCGCTC 1 cut(s) 1100
AciI CCGC 4 cut(s) 97, 676, 679, 1100
AclWI GGATC 1 cut(s) 807
AcsI RAATTY 4 cut(s) 232, 292, 1056, 1129
AcuI CTGAAG 1 cut(s) 326
AdeI CACNNNGTG 1 cut(s) 405
AfaI GTAC 1 cut(s) 969
AfiI CCNNNNNNNGG 7 cut(s) 405, 422, 423, 492, 796, 1120, 1292
AflIII ACRYGT 2 cut(s) 557, 1204
AgsI TTSAA 6 cut(s) 283, 298, 448, 783, 1062, 1335
AluBI AGCT 9 cut(s) 102, 141, 441, 659, 879, 1009, 1027, 1040, 1240
AluI AGCT 9 cut(s) 102, 141, 441, 659, 879, 1009, 1027, 1040, 1240
Alw21I GWGCWC 3 cut(s) 443, 557, 1042
Alw26I GTCTC 2 cut(s) 306, 735
Alw44I GTGCAC 1 cut(s) 553
AlwI GGATC 1 cut(s) 807
Ama87I CYCGRG 2 cut(s) 436, 874
AoxI GGCC 4 cut(s) 207, 321, 483, 527
ApaI GGGCCC 1 cut(s) 325
ApaLI GTGCAC 1 cut(s) 553
ApeKI GCWGC 6 cut(s) 99, 274, 315, 476, 1237, 1344
ApoI RAATTY 4 cut(s) 232, 292, 1056, 1129
AspS9I GGNCC 6 cut(s) 321, 322, 390, 409, 426, 646
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 4 cut(s) 115, 150, 659, 967
AvaI CYCGRG 2 cut(s) 436, 874
AvaII GGWCC 4 cut(s) 390, 409, 426, 646
BaeGI GKGCMC 3 cut(s) 325, 557, 605
BanII GRGCYC 3 cut(s) 325, 443, 1042
BauI CACGAG 1 cut(s) 1035
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 3 cut(s) 443, 557, 1042
BbvI GCAGC 6 cut(s) 111, 286, 302, 463, 1249, 1331
BccI CCATC 3 cut(s) 5, 590, 1264
BceAI ACGGC 2 cut(s) 56, 194
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 2 cut(s) 306, 735
BfaI CTAG 2 cut(s) 498, 656
BfmI CTRYAG 3 cut(s) 103, 316, 1235
BfuAI ACCTGC 1 cut(s) 943
BglII AGATCT 1 cut(s) 450
BisI GCNGC 8 cut(s) 97, 100, 275, 316, 477, 677, 1238, 1345
BlsI GCNGC 8 cut(s) 98, 101, 276, 317, 478, 678, 1239, 1346
Bme1390I CCNGG 1 cut(s) 482
Bme18I GGWCC 4 cut(s) 390, 409, 426, 646
BmeT110I CYCGRG 2 cut(s) 436, 874
BmgT120I GGNCC 6 cut(s) 321, 322, 390, 409, 426, 646
BmiI GGNNCC 5 cut(s) 323, 391, 410, 411, 427
BmrFI CCNGG 1 cut(s) 482
BmrI ACTGGG 1 cut(s) 1006
BmsI GCATC 3 cut(s) 123, 261, 1320
BmuI ACTGGG 1 cut(s) 1006
BpiI GAAGAC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 1166
BpuEI CTTGAG 2 cut(s) 311, 844
BpuMI CCSGG 1 cut(s) 482
BsaAI YACGTR 1 cut(s) 1207
BsaJI CCNNGG 4 cut(s) 38, 210, 287, 480
BsaWI WCCGGW 1 cut(s) 648
Bsc4I CCNNNNNNNGG 7 cut(s) 405, 422, 423, 492, 796, 1120, 1292
Bse1I ACTGG 3 cut(s) 316, 1001, 1149
Bse3DI GCAATG 4 cut(s) 192, 427, 515, 530
BseDI CCNNGG 4 cut(s) 38, 210, 287, 480
BseGI GGATG 2 cut(s) 16, 547
BseLI CCNNNNNNNGG 7 cut(s) 405, 422, 423, 492, 796, 1120, 1292
BseMI GCAATG 4 cut(s) 192, 427, 515, 530
BseMII CTCAG 5 cut(s) 360, 462, 684, 722, 1202
BseNI ACTGG 3 cut(s) 316, 1001, 1149
BseRI GAGGAG 1 cut(s) 325
BseSI GKGCMC 3 cut(s) 325, 557, 605
BseXI GCAGC 6 cut(s) 111, 286, 302, 463, 1249, 1331
BseYI CCCAGC 1 cut(s) 416
BsgI GTGCAG 1 cut(s) 1330
Bsh1285I CGRYCG 1 cut(s) 1168
BshFI GGCC 4 cut(s) 209, 323, 485, 529
BsiEI CGRYCG 1 cut(s) 1168
BsiHKAI GWGCWC 3 cut(s) 443, 557, 1042
BsiHKCI CYCGRG 2 cut(s) 436, 874
BsiSI CCGG 2 cut(s) 482, 649
BslFI GGGAC 4 cut(s) 422, 470, 798, 996
BslI CCNNNNNNNGG 7 cut(s) 405, 422, 423, 492, 796, 1120, 1292
BsmAI GTCTC 2 cut(s) 306, 735
BsmFI GGGAC 4 cut(s) 422, 470, 798, 996
BsnI GGCC 4 cut(s) 209, 323, 485, 529
BsoBI CYCGRG 2 cut(s) 436, 874
Bsp120I GGGCCC 1 cut(s) 321
Bsp1286I GDGCHC 5 cut(s) 325, 443, 557, 605, 1042
Bsp143I GATC 4 cut(s) 450, 799, 957, 1165
BspACI CCGC 4 cut(s) 97, 676, 679, 1100
BspANI GGCC 4 cut(s) 209, 323, 485, 529
BspCNI CTCAG 5 cut(s) 361, 463, 685, 721, 1201
BspLI GGNNCC 5 cut(s) 323, 391, 410, 411, 427
BspMAI CTGCAG 2 cut(s) 320, 1239
BspMI ACCTGC 1 cut(s) 943
BspPI GGATC 1 cut(s) 807
BspQI GCTCTTC 1 cut(s) 1090
BsrBI CCGCTC 1 cut(s) 1100
BsrDI GCAATG 4 cut(s) 192, 427, 515, 530
BsrI ACTGG 3 cut(s) 316, 1001, 1149
BssECI CCNNGG 4 cut(s) 38, 210, 287, 480
BssMI GATC 4 cut(s) 450, 799, 957, 1165
BssSI CACGAG 1 cut(s) 1035
Bst2BI CACGAG 1 cut(s) 1035
Bst4CI ACNGT 2 cut(s) 967, 1351
Bst6I CTCTTC 1 cut(s) 1090
BstBAI YACGTR 1 cut(s) 1207
BstC8I GCNNGC 3 cut(s) 207, 357, 527
BstDEI CTNAG 6 cut(s) 369, 471, 693, 708, 1106, 1188
BstDSI CCRYGG 2 cut(s) 38, 210
BstENI CCTNNNNNAGG 1 cut(s) 1290
BstF5I GGATG 2 cut(s) 16, 547
BstKTI GATC 4 cut(s) 453, 802, 960, 1168
BstMAI GTCTC 2 cut(s) 306, 735
BstMBI GATC 4 cut(s) 450, 799, 957, 1165
BstMCI CGRYCG 1 cut(s) 1168
BstMWI GCNNNNNNNGC 7 cut(s) 105, 196, 283, 337, 482, 526, 854
BstNSI RCATGY 1 cut(s) 561
BstSCI CCNGG 1 cut(s) 480
BstSFI CTRYAG 3 cut(s) 103, 316, 1235
BstSLI GKGCMC 3 cut(s) 325, 557, 605
BstV1I GCAGC 6 cut(s) 111, 286, 302, 463, 1249, 1331
BstV2I GAAGAC 1 cut(s) 249
BstX2I RGATCY 1 cut(s) 450
BstXI CCANNNNNNTGG 1 cut(s) 996
BstYI RGATCY 1 cut(s) 450
BsuRI GGCC 4 cut(s) 209, 323, 485, 529
BtgI CCRYGG 2 cut(s) 38, 210
BtgZI GCGATG 1 cut(s) 100
BtsCI GGATG 2 cut(s) 16, 547
BtsIMutI CAGTG 2 cut(s) 309, 1142
BveI ACCTGC 1 cut(s) 943
Cac8I GCNNGC 3 cut(s) 207, 357, 527
Cfr13I GGNCC 6 cut(s) 321, 322, 390, 409, 426, 646
Csp6I GTAC 1 cut(s) 968
CspCI CAANNNNNGTGG 2 cut(s) 525, 560
CviAII CATG 3 cut(s) 558, 775, 931
CviQI GTAC 1 cut(s) 968
DdeI CTNAG 6 cut(s) 369, 471, 693, 708, 1106, 1188
DpnI GATC 4 cut(s) 452, 801, 959, 1167
DpnII GATC 4 cut(s) 450, 799, 957, 1165
DraIII CACNNNGTG 1 cut(s) 405
Eam1104I CTCTTC 1 cut(s) 1090
EarI CTCTTC 1 cut(s) 1090
EciI GGCGGA 1 cut(s) 694
Ecl136II GAGCTC 2 cut(s) 441, 1040
Eco24I GRGCYC 3 cut(s) 325, 443, 1042
Eco32I GATATC 1 cut(s) 1283
Eco47I GGWCC 4 cut(s) 390, 409, 426, 646
Eco53kI GAGCTC 2 cut(s) 441, 1040
Eco57I CTGAAG 1 cut(s) 326
Eco88I CYCGRG 2 cut(s) 436, 874
EcoICRI GAGCTC 2 cut(s) 441, 1040
EcoNI CCTNNNNNAGG 1 cut(s) 1290
EcoO109I RGGNCCY 3 cut(s) 321, 322, 409
EcoRV GATATC 1 cut(s) 1283
EcoT38I GRGCYC 3 cut(s) 325, 443, 1042
FaeI CATG 3 cut(s) 561, 778, 934
FalI AAGNNNNNCTT 4 cut(s) 365, 397, 993, 1025
FaqI GGGAC 4 cut(s) 422, 470, 798, 996
FatI CATG 3 cut(s) 557, 774, 930
FauI CCCGC 1 cut(s) 1093
FauNDI CATATG 1 cut(s) 794
Fnu4HI GCNGC 8 cut(s) 97, 100, 275, 316, 477, 677, 1238, 1345
FokI GGATG 2 cut(s) 23, 554
FriOI GRGCYC 3 cut(s) 325, 443, 1042
Fsp4HI GCNGC 8 cut(s) 97, 100, 275, 316, 477, 677, 1238, 1345
FspBI CTAG 2 cut(s) 498, 656
GluI GCNGC 8 cut(s) 97, 100, 275, 316, 477, 677, 1238, 1345
GsaI CCCAGC 1 cut(s) 420
GsuI CTGGAG 1 cut(s) 1166
HaeIII GGCC 4 cut(s) 209, 323, 485, 529
HapII CCGG 2 cut(s) 482, 649
Hin1II CATG 3 cut(s) 561, 778, 934
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HindIII AAGCTT 1 cut(s) 1007
HinfI GANTC 4 cut(s) 467, 1110, 1172, 1331
HpaI GTTAAC 1 cut(s) 699
HpaII CCGG 2 cut(s) 482, 649
HphI GGTGA 4 cut(s) 115, 150, 659, 967
Hpy166II GTNNAC 4 cut(s) 309, 555, 699, 861
Hpy188I TCNGA 9 cut(s) 430, 466, 472, 694, 711, 739, 1177, 1200, 1244
Hpy188III TCNNGA 5 cut(s) 874, 1035, 1126, 1169, 1252
Hpy8I GTNNAC 4 cut(s) 309, 555, 699, 861
HpyCH4III ACNGT 2 cut(s) 967, 1351
HpyCH4IV ACGT 2 cut(s) 898, 1206
HpyF10VI GCNNNNNNNGC 7 cut(s) 105, 196, 283, 337, 482, 526, 854
HpyF3I CTNAG 6 cut(s) 369, 471, 693, 708, 1106, 1188
HpySE526I ACGT 2 cut(s) 898, 1206
Hsp92II CATG 3 cut(s) 561, 778, 934
KflI GGGWCCC 1 cut(s) 409
KspAI GTTAAC 1 cut(s) 699
Kzo9I GATC 4 cut(s) 450, 799, 957, 1165
LguI GCTCTTC 1 cut(s) 1090
LmnI GCTCC 1 cut(s) 1045
Lsp1109I GCAGC 6 cut(s) 111, 286, 302, 463, 1249, 1331
LweI GCATC 3 cut(s) 123, 261, 1320
MaeI CTAG 2 cut(s) 498, 656
MaeII ACGT 2 cut(s) 898, 1206
MaeIII GTNAC 2 cut(s) 890, 1150
MalI GATC 4 cut(s) 452, 801, 959, 1167
MbiI CCGCTC 1 cut(s) 1100
MboI GATC 4 cut(s) 450, 799, 957, 1165
MboII GAAGA 3 cut(s) 249, 460, 1107
MflI RGATCY 1 cut(s) 450
MhlI GDGCHC 5 cut(s) 325, 443, 557, 605, 1042
MluCI AATT 7 cut(s) 33, 232, 292, 817, 1056, 1129, 1230
MmeI TCCRAC 2 cut(s) 453, 1159
MseI TTAA 5 cut(s) 164, 698, 938, 962, 978
MslI CAYNNNNRTG 2 cut(s) 738, 1209
MspI CCGG 2 cut(s) 482, 649
MspR9I CCNGG 1 cut(s) 482
MwoI GCNNNNNNNGC 7 cut(s) 105, 196, 283, 337, 482, 526, 854
NciI CCSGG 1 cut(s) 482
NdeI CATATG 1 cut(s) 794
NdeII GATC 4 cut(s) 450, 799, 957, 1165
NlaIII CATG 3 cut(s) 561, 778, 934
NlaIV GGNNCC 5 cut(s) 323, 391, 410, 411, 427
NmeAIII GCCGAG 1 cut(s) 312
NspI RCATGY 1 cut(s) 561
PaeR7I CTCGAG 2 cut(s) 436, 874
PaqCI CACCTGC 1 cut(s) 943
PciI ACATGT 1 cut(s) 557
PciSI GCTCTTC 1 cut(s) 1090
PfeI GAWTC 4 cut(s) 467, 1110, 1172, 1331
PflMI CCANNNNNTGG 1 cut(s) 405
PkrI GCNGC 8 cut(s) 98, 101, 276, 317, 478, 678, 1239, 1346
Ple19I CGATCG 1 cut(s) 1168
Ppu21I YACGTR 1 cut(s) 1207
PpuMI RGGWCCY 1 cut(s) 409
PscI ACATGT 1 cut(s) 557
Psp124BI GAGCTC 2 cut(s) 443, 1042
Psp5II RGGWCCY 1 cut(s) 409
PspFI CCCAGC 1 cut(s) 416
PspN4I GGNNCC 5 cut(s) 323, 391, 410, 411, 427
PspOMI GGGCCC 1 cut(s) 321
PspPI GGNCC 6 cut(s) 321, 322, 390, 409, 426, 646
PspPPI RGGWCCY 1 cut(s) 409
PspXI VCTCGAGB 1 cut(s) 436
PstI CTGCAG 2 cut(s) 320, 1239
PsuI RGATCY 1 cut(s) 450
PvuI CGATCG 1 cut(s) 1168
RsaI GTAC 1 cut(s) 969
RsaNI GTAC 1 cut(s) 968
RseI CAYNNNNRTG 2 cut(s) 738, 1209
SacI GAGCTC 2 cut(s) 443, 1042
SapI GCTCTTC 1 cut(s) 1090
SaqAI TTAA 5 cut(s) 164, 698, 938, 962, 978
SatI GCNGC 8 cut(s) 97, 100, 275, 316, 477, 677, 1238, 1345
Sau3AI GATC 4 cut(s) 450, 799, 957, 1165
Sau96I GGNCC 6 cut(s) 321, 322, 390, 409, 426, 646
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 5 cut(s) 325, 443, 557, 605, 1042
SfaNI GCATC 3 cut(s) 123, 261, 1320
SfcI CTRYAG 3 cut(s) 103, 316, 1235
Sfr274I CTCGAG 2 cut(s) 436, 874
SinI GGWCC 4 cut(s) 390, 409, 426, 646
SlaI CTCGAG 2 cut(s) 436, 874
SmiMI CAYNNNNRTG 2 cut(s) 738, 1209
SmlI CTYRAG 4 cut(s) 326, 436, 823, 874
SmoI CTYRAG 4 cut(s) 326, 436, 823, 874
Sse9I AATT 7 cut(s) 33, 232, 292, 817, 1056, 1129, 1230
SsiI CCGC 4 cut(s) 97, 676, 679, 1100
SspMI CTAG 2 cut(s) 498, 656
SstI GAGCTC 2 cut(s) 443, 1042
StyD4I CCNGG 1 cut(s) 480
TaaI ACNGT 2 cut(s) 967, 1351
TaiI ACGT 2 cut(s) 901, 1209
TaqI TCGA 4 cut(s) 143, 437, 875, 1259
TaqII GACCGA 1 cut(s) 1111
TasI AATT 7 cut(s) 33, 232, 292, 817, 1056, 1129, 1230
TatI WGTACW 1 cut(s) 967
TauI GCSGC 2 cut(s) 99, 679
TfiI GAWTC 4 cut(s) 467, 1110, 1172, 1331
Tru1I TTAA 5 cut(s) 164, 698, 938, 962, 978
Tru9I TTAA 5 cut(s) 164, 698, 938, 962, 978
TscAI CASTG 2 cut(s) 316, 1149
TseI GCWGC 6 cut(s) 99, 274, 315, 476, 1237, 1344
TspDTI ATGAA 4 cut(s) 642, 1010, 1175, 1227
TspRI CASTG 2 cut(s) 316, 1149
Van91I CCANNNNNTGG 1 cut(s) 405
VneI GTGCAC 1 cut(s) 553
VpaK11BI GGWCC 4 cut(s) 390, 409, 426, 646
XagI CCTNNNNNAGG 1 cut(s) 1290
XapI RAATTY 4 cut(s) 232, 292, 1056, 1129
XceI RCATGY 1 cut(s) 561
XhoI CTCGAG 2 cut(s) 436, 874
XspI CTAG 2 cut(s) 498, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.