pycom09g18190

Nudix hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0013600
Physical Location & Seq
Forward (+)
18791103 .. 18792301
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
ATGGTAGCTTTGATACCTCAAGAAAACACGGTTTCTGCTTTGGTAGCTCGCACTGGAAGGCATTTGCAGCGTTACAGCAAAGGTCGTCGCCAAGTTGTCGGATGTATTCCATATAGATATAAAGCTTCCAAGCAAACTCCCTTGAAGGATGATGCACAAGAACTTGAAGTCCTTGTCATTAGTTCACAGAAGGGCAAAGGGATGCTCTTTCCAAAGGGAGGGTGGGAACAGGACGAATCCAAGGAAGGGGCAGCATCGCGAGAGACGCTAGAGGAAGCAGGAGTACGAGGACTCATTGGGTGTGAATTGGGTAAATGGAGTTTCAAGAGCAAAAGCCATGATTCTGACTATGAAGGCTACATGTTCCCTTTACTTGTTCAGGAGCAGTTAGATTTCTGGCCAGAGAAGAACCTCAGGCAAAGAGTTTGGATGAATGTTGAAGAAGCAAGAGAAGTCTGTCAGCATTGGTGGATGAAAGAAGCTTTGGATAGATTAGTATATCGACTAACTCAGCAACAAAAGCATCAAGGCCCTCTTCTTAAATCTAGGAAGGAAAATCTGATGGCATGTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUDIX

Protein Analysis

192

Amino Acids

22.11

Weight (kDa)

9.0

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX PF00293 53 - 163 4.3e-10 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 259
AcoI YGGCCR 1 cut(s) 398
AfaI GTAC 2 cut(s) 285, 571
AfiI CCNNNNNNNGG 2 cut(s) 218, 246
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 4 cut(s) 145, 167, 325, 440
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AloI GAACNNNNNNTCC 2 cut(s) 153, 185
AluBI AGCT 4 cut(s) 8, 47, 125, 482
AluI AGCT 4 cut(s) 8, 47, 125, 482
Alw26I GTCTC 1 cut(s) 257
AoxI GGCC 2 cut(s) 398, 529
ApeKI GCWGC 2 cut(s) 67, 251
AspS9I GGNCC 1 cut(s) 530
AxyI CCTNAGG 1 cut(s) 413
BalI TGGCCA 1 cut(s) 400
BarI GAAGNNNNNNTAC 2 cut(s) 267, 299
BbvI GCAGC 2 cut(s) 79, 263
BccI CCATC 1 cut(s) 556
BcoDI GTCTC 1 cut(s) 257
BfaI CTAG 2 cut(s) 269, 546
BisI GCNGC 2 cut(s) 68, 252
BlsI GCNGC 2 cut(s) 69, 253
BmgT120I GGNCC 1 cut(s) 530
BmsI GCATC 4 cut(s) 142, 192, 263, 532
BsaJI CCNNGG 1 cut(s) 240
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 246
Bse1I ACTGG 1 cut(s) 58
Bse21I CCTNAGG 1 cut(s) 413
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 5 cut(s) 107, 154, 207, 435, 477
BseLI CCNNNNNNNGG 2 cut(s) 218, 246
BseMII CTCAG 2 cut(s) 427, 524
BseNI ACTGG 1 cut(s) 58
BseXI GCAGC 2 cut(s) 79, 263
Bsh1236I CGCG 1 cut(s) 259
BshFI GGCC 2 cut(s) 400, 531
BslI CCNNNNNNNGG 2 cut(s) 218, 246
BsmAI GTCTC 1 cut(s) 257
BsmBI CGTCTC 1 cut(s) 257
BsnI GGCC 2 cut(s) 400, 531
Bsp1407I TGTACA 1 cut(s) 569
Bsp68I TCGCGA 1 cut(s) 259
BspANI GGCC 2 cut(s) 400, 531
BspCNI CTCAG 2 cut(s) 426, 523
BspFNI CGCG 1 cut(s) 259
BsrGI TGTACA 1 cut(s) 569
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 540
BstAUI TGTACA 1 cut(s) 569
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 2 cut(s) 413, 510
BstF5I GGATG 5 cut(s) 107, 154, 207, 435, 477
BstFNI CGCG 1 cut(s) 259
BstMAI GTCTC 1 cut(s) 257
BstMWI GCNNNNNNNGC 4 cut(s) 44, 67, 265, 520
BstNSI RCATGY 2 cut(s) 364, 570
BstUI CGCG 1 cut(s) 259
BstV1I GCAGC 2 cut(s) 79, 263
Bsu36I CCTNAGG 1 cut(s) 413
BsuRI GGCC 2 cut(s) 400, 531
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 5 cut(s) 107, 154, 207, 435, 477
BtsIMutI CAGTG 1 cut(s) 51
BtuMI TCGCGA 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 530
CseI GACGC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 284, 570
CviAII CATG 3 cut(s) 338, 361, 567
CviJI RGCY 8 cut(s) 8, 47, 125, 336, 357, 400, 482, 531
CviKI_1 RGCY 8 cut(s) 8, 47, 125, 336, 357, 400, 482, 531
CviQI GTAC 2 cut(s) 284, 570
DdeI CTNAG 2 cut(s) 413, 510
EaeI YGGCCR 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
Eco130I CCWWGG 1 cut(s) 240
Eco81I CCTNAGG 1 cut(s) 413
EcoO109I RGGNCCY 1 cut(s) 530
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 3 cut(s) 341, 364, 570
FaiI YATR 9 cut(s) 112, 114, 120, 339, 351, 362, 499, 568, 574
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FatI CATG 3 cut(s) 337, 360, 566
Fnu4HI GCNGC 2 cut(s) 68, 252
FokI GGATG 5 cut(s) 114, 161, 214, 442, 484
Fsp4HI GCNGC 2 cut(s) 68, 252
FspBI CTAG 2 cut(s) 269, 546
GluI GCNGC 2 cut(s) 68, 252
HaeIII GGCC 2 cut(s) 400, 531
HgaI GACGC 1 cut(s) 274
Hin1II CATG 3 cut(s) 341, 364, 570
HindIII AAGCTT 2 cut(s) 123, 480
HinfI GANTC 3 cut(s) 236, 291, 341
Hpy166II GTNNAC 1 cut(s) 185
Hpy188I TCNGA 3 cut(s) 101, 346, 561
Hpy188III TCNNGA 4 cut(s) 20, 258, 325, 380
Hpy8I GTNNAC 1 cut(s) 185
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 6 cut(s) 51, 139, 184, 239, 347, 544
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 2 cut(s) 67, 155
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 67, 265, 520
HpyF3I CTNAG 2 cut(s) 413, 510
Hsp92II CATG 3 cut(s) 341, 364, 570
LmnI GCTCC 1 cut(s) 382
LpnPI CCDG 7 cut(s) 39, 215, 264, 365, 382, 400, 414
Lsp1109I GCAGC 2 cut(s) 79, 263
LweI GCATC 4 cut(s) 142, 192, 263, 532
MaeI CTAG 2 cut(s) 269, 546
MaeIII GTNAC 1 cut(s) 71
MboII GAAGA 3 cut(s) 418, 452, 527
MlsI TGGCCA 1 cut(s) 400
MluCI AATT 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 400
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 1 cut(s) 79
MnlI CCTC 6 cut(s) 27, 212, 265, 281, 422, 543
Mox20I TGGCCA 1 cut(s) 400
MscI TGGCCA 1 cut(s) 400
MseI TTAA 1 cut(s) 540
Msp20I TGGCCA 1 cut(s) 400
MvnI CGCG 1 cut(s) 259
MwoI GCNNNNNNNGC 4 cut(s) 44, 67, 265, 520
NlaIII CATG 3 cut(s) 341, 364, 570
NruI TCGCGA 1 cut(s) 259
NspI RCATGY 2 cut(s) 364, 570
PciI ACATGT 1 cut(s) 360
PfeI GAWTC 2 cut(s) 236, 341
PkrI GCNGC 2 cut(s) 69, 253
PleI GAGTC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 285
PscI ACATGT 1 cut(s) 360
PspPI GGNCC 1 cut(s) 530
RruI TCGCGA 1 cut(s) 259
RsaI GTAC 2 cut(s) 285, 571
RsaNI GTAC 2 cut(s) 284, 570
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 2 cut(s) 68, 252
Sau96I GGNCC 1 cut(s) 530
SchI GAGTC 1 cut(s) 285
SetI ASST 7 cut(s) 10, 19, 49, 85, 127, 414, 484
SfaNI GCATC 4 cut(s) 142, 192, 263, 532
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 1 cut(s) 305
SspMI CTAG 2 cut(s) 269, 546
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 31
TaqI TCGA 1 cut(s) 502
TasI AATT 1 cut(s) 305
TatI WGTACW 1 cut(s) 569
TfiI GAWTC 2 cut(s) 236, 341
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 1 cut(s) 58
TseI GCWGC 2 cut(s) 67, 251
TspDTI ATGAA 3 cut(s) 366, 446, 488
TspRI CASTG 1 cut(s) 58
XceI RCATGY 2 cut(s) 364, 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XspI CTAG 2 cut(s) 269, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.