pycom10g00290

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006215
Physical Location & Seq
Reverse (-)
377191 .. 380400
3210 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1494 bp
ATGGCAATGAGATTATCATTAAGCATCTTCATCTCTCTCTTTGTTATATTGGGCAGTAGTATTAGTGGTAGTGTCCAAGTCCTAGCTGCAGGCAACCCTAATTACAGAGAGGCCCTGGCAAAGTCCTTACTCTTCTTCCAGGGACAAAGGTCAGGGAGGCTCCCCGCCGGCGCTGCCCAGCAAATCACATGGAGGTCCAATTCTGGACTCTCCGATGGTCGTCAGGCATATGTGGACTTAACTGGAGGATACTATGACGCTGGAGACAATGTCAAGTTCAACTTCCCAATGGCATTCACCACCACAATGCTGTCATGGGGAGCACTTGAATATGGCAAGCGCATGGGCCCTCAGTTACCAGAAACCCGCGAGGCAATCCGGTGGGCCACAGATTACCTTATAAAGTGTGCTAGGCAAACACCCGGCAGACTCTACGTTGGGGTCGGTGACCCTAACGTAGACCATAAATGTTGGGAGAGGCCCGAAGACATGGACACTACAAGAACCGTGTACTCAGTCTCACCAAGCAATCCGGGCTCGGATGTTGCTGGAGAGACAGCCGCTGCATTAGCGGCCGCTTCTTTAGTTTTCCGGAGGGTTGACCCAAAATATTCAAAGTTGCTGCTCAACACGGCCAGGAACGTGATGCAATTTGCAATTCAGTACCGAGGTGCATACAGTGATTCCCTTGGGTCTGCAGTCTGCCCATTTTATTGCTCATATTCTGGATATAATGATGAGCTGCTGTGGGGAGCTGCATGGCTTTTTAGAGCAACTAATGAACTTTATTATTACAATTTTATAAAATCCTTGGGAGCCAGTGATTCAACGGATATTTTCAGCTGGGACAACAAATTTGCTGGTGCTTATGTTTTACTATCAAGGAGGGCACTCTTGAATAACGACAAGAACTTTGAGCCATATAAACAAGAAGCTGAGCAATTTATGTGTCGGATTTTGCCAAACTCACCCTCTTCAAGTACTCAATATACACAAGGAGGGCTCATATACAAGCTACCTGGAAGTAACCTCCAATATGTGACCTCAATAACATTCTTGCTTACTACCTATGCCAAGTACATGGCTGCTAGAAAGCTCACATTTGACTGTGGTAACCTCGTTGTCACTCCGATGGCCTTAAGAAACCTTGCAAAACAACAGGTGGACTACATATTAGGGGTAAACCCTCTGAAGATGTCCTACATGGTTGGGTATGGCCCCTACTATCCCAAGAGAATTCACCACAGACGATCCTCATTGCCTTCATTGACAAGCCATCGCCAGAGCATAGGTTGCGATGGTGGTTTCCAACCATTCTTCTATTCGCTAAACCCAAATCCCAACATCTTAGTTGGAGCCATTGTTGGAGGTCCAAACCAGAACGATGGATTCCCAGATGATCGCGGCGATTACAGCCATTCGGAGCCTGCAACGTACATAAATGGCGCTATTGTAGGGCCCTTGGCATTCTTTGCAGGGAGCTACAGAAGTTGA

Protein Analysis

498

Amino Acids

55.02

Weight (kDa)

9.0

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 35 - 486 3.8e-156 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 401, 803
AccI GTMKAC 1 cut(s) 459
AccII CGCG 2 cut(s) 369, 1404
AccIII TCCGGA 1 cut(s) 591
AciI CCGC 6 cut(s) 165, 367, 561, 572, 576, 1404
AclWI GGATC 1 cut(s) 1245
AcoI YGGCCR 2 cut(s) 573, 633
AcsI RAATTY 2 cut(s) 854, 1236
AcuI CTGAAG 1 cut(s) 1211
AfaI GTAC 5 cut(s) 512, 665, 982, 1079, 1436
AflII CTTAAG 1 cut(s) 1138
AgsI TTSAA 6 cut(s) 280, 329, 615, 828, 898, 978
AjnI CCWGG 4 cut(s) 114, 138, 635, 1018
AluBI AGCT 8 cut(s) 86, 742, 755, 843, 935, 1015, 1096, 1482
AluI AGCT 8 cut(s) 86, 742, 755, 843, 935, 1015, 1096, 1482
Alw21I GWGCWC 1 cut(s) 325
Alw26I GTCTC 3 cut(s) 258, 523, 548
AlwI GGATC 1 cut(s) 1245
AlwNI CAGNNNCTG 1 cut(s) 563
Aor13HI TCCGGA 1 cut(s) 591
AoxI GGCC 9 cut(s) 111, 346, 384, 479, 573, 633, 1134, 1216, 1457
ApaI GGGCCC 2 cut(s) 350, 1461
ApeKI GCWGC 7 cut(s) 86, 173, 563, 622, 742, 755, 1085
ApoI RAATTY 2 cut(s) 854, 1236
ArsI GACNNNNNNTTYG 4 cut(s) 839, 871, 896, 928
AspLEI GCGC 3 cut(s) 173, 342, 1448
AsuC2I CCSGG 2 cut(s) 423, 534
AsuHPI GGTGA 5 cut(s) 289, 458, 513, 960, 1232
AvaII GGWCC 2 cut(s) 195, 1370
BaeGI GKGCMC 3 cut(s) 350, 892, 1461
BanII GRGCYC 4 cut(s) 350, 539, 1005, 1461
BarI GAAGNNNNNNTAC 2 cut(s) 1184, 1216
BbsI GAAGAC 1 cut(s) 492
Bbv12I GWGCWC 1 cut(s) 325
BbvI GCAGC 7 cut(s) 73, 160, 550, 609, 729, 742, 1072
BccI CCATC 5 cut(s) 209, 1126, 1284, 1292, 1379
BceAI ACGGC 1 cut(s) 648
BciT130I CCWGG 4 cut(s) 116, 140, 637, 1020
BciVI GTATCC 1 cut(s) 242
BcnI CCSGG 2 cut(s) 423, 534
BcoDI GTCTC 3 cut(s) 258, 523, 548
BfaI CTAG 3 cut(s) 83, 411, 1089
BfmI CTRYAG 3 cut(s) 87, 696, 1483
BfoI RGCGCY 2 cut(s) 174, 1449
BfrI CTTAAG 1 cut(s) 1138
BfuI GTATCC 1 cut(s) 242
BlpI GCTNAGC 1 cut(s) 936
BmcAI AGTACT 1 cut(s) 982
Bme1390I CCNGG 6 cut(s) 116, 140, 423, 534, 637, 1020
Bme18I GGWCC 2 cut(s) 195, 1370
BmiI GGNNCC 7 cut(s) 161, 348, 817, 1219, 1357, 1425, 1459
BmrFI CCNGG 6 cut(s) 116, 140, 423, 534, 637, 1020
BmsI GCATC 2 cut(s) 33, 636
BoxI GACNNNNGTC 1 cut(s) 148
BpiI GAAGAC 1 cut(s) 492
BpmI CTGGAG 3 cut(s) 264, 282, 570
Bpu1102I GCTNAGC 1 cut(s) 936
BpuMI CCSGG 2 cut(s) 423, 534
BsaJI CCNNGG 6 cut(s) 114, 139, 667, 688, 810, 1461
BsaWI WCCGGW 2 cut(s) 378, 591
BsaXI ACNNNNNCTCC 4 cut(s) 255, 285, 1347, 1377
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 247, 819
Bse3DI GCAATG 2 cut(s) 12, 1256
BseAI TCCGGA 1 cut(s) 591
BseBI CCWGG 4 cut(s) 116, 140, 637, 1020
BseDI CCNNGG 6 cut(s) 114, 139, 667, 688, 810, 1461
BseGI GGATG 1 cut(s) 547
BseMI GCAATG 2 cut(s) 12, 1256
BseMII CTCAG 3 cut(s) 365, 528, 927
BseNI ACTGG 2 cut(s) 247, 819
BseSI GKGCMC 3 cut(s) 350, 892, 1461
BseX3I CGGCCG 1 cut(s) 573
BseXI GCAGC 7 cut(s) 73, 160, 550, 609, 729, 742, 1072
BseYI CCCAGC 2 cut(s) 177, 843
Bsh1236I CGCG 2 cut(s) 369, 1404
Bsh1285I CGRYCG 1 cut(s) 576
BshFI GGCC 9 cut(s) 113, 348, 386, 481, 575, 635, 1136, 1218, 1459
BsiEI CGRYCG 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 325
BsiSI CCGG 5 cut(s) 168, 379, 423, 533, 592
BslFI GGGAC 2 cut(s) 156, 860
BsmAI GTCTC 3 cut(s) 258, 523, 548
BsmFI GGGAC 2 cut(s) 156, 860
BsmI GAATGC 2 cut(s) 293, 1466
BsnI GGCC 9 cut(s) 113, 348, 386, 481, 575, 635, 1136, 1218, 1459
Bsp120I GGGCCC 2 cut(s) 346, 1457
Bsp1286I GDGCHC 6 cut(s) 325, 350, 539, 892, 1005, 1461
Bsp13I TCCGGA 1 cut(s) 591
Bsp143I GATC 2 cut(s) 1250, 1399
Bsp1720I GCTNAGC 1 cut(s) 936
BspACI CCGC 6 cut(s) 165, 367, 561, 572, 576, 1404
BspANI GGCC 9 cut(s) 113, 348, 386, 481, 575, 635, 1136, 1218, 1459
BspCNI CTCAG 3 cut(s) 364, 527, 928
BspEI TCCGGA 1 cut(s) 591
BspFNI CGCG 2 cut(s) 369, 1404
BspLI GGNNCC 7 cut(s) 161, 348, 817, 1219, 1357, 1425, 1459
BspMAI CTGCAG 2 cut(s) 91, 700
BspPI GGATC 1 cut(s) 1245
BspTI CTTAAG 1 cut(s) 1138
BsrDI GCAATG 2 cut(s) 12, 1256
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 2 cut(s) 247, 819
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 6 cut(s) 114, 139, 667, 688, 810, 1461
BssMI GATC 2 cut(s) 1250, 1399
BssT1I CCWWGG 3 cut(s) 688, 810, 1461
Bst2UI CCWGG 4 cut(s) 116, 140, 637, 1020
Bst4CI ACNGT 3 cut(s) 508, 680, 1109
Bst6I CTCTTC 2 cut(s) 137, 979
BstAFI CTTAAG 1 cut(s) 1138
BstAPI GCANNNNNTGC 2 cut(s) 1293, 1472
BstC8I GCNNGC 4 cut(s) 91, 169, 338, 1428
BstDEI CTNAG 4 cut(s) 351, 514, 936, 1348
BstEII GGTNACC 2 cut(s) 446, 1112
BstF5I GGATG 1 cut(s) 547
BstFNI CGCG 2 cut(s) 369, 1404
BstH2I RGCGCY 2 cut(s) 174, 1449
BstHHI GCGC 3 cut(s) 173, 342, 1448
BstKTI GATC 2 cut(s) 1253, 1402
BstMAI GTCTC 3 cut(s) 258, 523, 548
BstMBI GATC 2 cut(s) 1250, 1399
BstMCI CGRYCG 1 cut(s) 576
BstMWI GCNNNNNNNGC 7 cut(s) 173, 534, 569, 572, 1293, 1413, 1472
BstNI CCWGG 4 cut(s) 116, 140, 637, 1020
BstPAI GACNNNNGTC 1 cut(s) 148
BstPI GGTNACC 2 cut(s) 446, 1112
BstSCI CCNGG 6 cut(s) 114, 138, 421, 532, 635, 1018
BstSFI CTRYAG 3 cut(s) 87, 696, 1483
BstSLI GKGCMC 3 cut(s) 350, 892, 1461
BstUI CGCG 2 cut(s) 369, 1404
BstV1I GCAGC 7 cut(s) 73, 160, 550, 609, 729, 742, 1072
BstV2I GAAGAC 1 cut(s) 492
BstXI CCANNNNNNTGG 2 cut(s) 1081, 1385
BstZI CGGCCG 1 cut(s) 573
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 9 cut(s) 113, 348, 386, 481, 575, 635, 1136, 1218, 1459
BtgZI GCGATG 2 cut(s) 1262, 1311
BtsCI GGATG 1 cut(s) 547
BtsIMutI CAGTG 2 cut(s) 685, 826
Cac8I GCNNGC 4 cut(s) 91, 169, 338, 1428
CaiI CAGNNNCTG 1 cut(s) 563
CciNI GCGGCCGC 1 cut(s) 573
CfoI GCGC 3 cut(s) 173, 342, 1448
Cfr10I RCCGGY 1 cut(s) 167
CseI GACGC 1 cut(s) 266
Csp6I GTAC 5 cut(s) 511, 664, 981, 1078, 1435
CviAII CATG 7 cut(s) 189, 315, 343, 490, 759, 1081, 1204
CviQI GTAC 5 cut(s) 511, 664, 981, 1078, 1435
DdeI CTNAG 4 cut(s) 351, 514, 936, 1348
DpnI GATC 2 cut(s) 1252, 1401
DpnII GATC 2 cut(s) 1250, 1399
EaeI YGGCCR 2 cut(s) 573, 633
EagI CGGCCG 1 cut(s) 573
Eam1104I CTCTTC 2 cut(s) 137, 979
EarI CTCTTC 2 cut(s) 137, 979
EclXI CGGCCG 1 cut(s) 573
Eco130I CCWWGG 3 cut(s) 688, 810, 1461
Eco24I GRGCYC 4 cut(s) 350, 539, 1005, 1461
Eco47I GGWCC 2 cut(s) 195, 1370
Eco52I CGGCCG 1 cut(s) 573
Eco57I CTGAAG 1 cut(s) 1211
Eco91I GGTNACC 2 cut(s) 446, 1112
EcoO109I RGGNCCY 4 cut(s) 112, 347, 1457, 1458
EcoO65I GGTNACC 2 cut(s) 446, 1112
EcoRI GAATTC 1 cut(s) 1236
EcoRII CCWGG 4 cut(s) 114, 138, 635, 1018
EcoT14I CCWWGG 3 cut(s) 688, 810, 1461
EcoT38I GRGCYC 4 cut(s) 350, 539, 1005, 1461
ErhI CCWWGG 3 cut(s) 688, 810, 1461
FaeI CATG 7 cut(s) 192, 318, 346, 493, 762, 1084, 1207
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FaqI GGGAC 2 cut(s) 156, 860
FatI CATG 7 cut(s) 188, 314, 342, 489, 758, 1080, 1203
FauI CCCGC 2 cut(s) 172, 374
FauNDI CATATG 1 cut(s) 229
FblI GTMKAC 1 cut(s) 459
FokI GGATG 1 cut(s) 554
FriOI GRGCYC 4 cut(s) 350, 539, 1005, 1461
FspBI CTAG 3 cut(s) 83, 411, 1089
GlaI GCGC 3 cut(s) 172, 341, 1447
GsaI CCCAGC 2 cut(s) 181, 847
GsuI CTGGAG 3 cut(s) 264, 282, 570
HaeII RGCGCY 2 cut(s) 174, 1449
HaeIII GGCC 9 cut(s) 113, 348, 386, 481, 575, 635, 1136, 1218, 1459
HapII CCGG 5 cut(s) 168, 379, 423, 533, 592
HgaI GACGC 1 cut(s) 266
HhaI GCGC 3 cut(s) 173, 342, 1448
Hin1II CATG 7 cut(s) 192, 318, 346, 493, 762, 1084, 1207
Hin6I GCGC 3 cut(s) 171, 340, 1446
HinP1I GCGC 3 cut(s) 171, 340, 1446
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 5 cut(s) 207, 429, 683, 824, 1389
HpaII CCGG 5 cut(s) 168, 379, 423, 533, 592
HphI GGTGA 5 cut(s) 289, 458, 513, 960, 1232
Hpy166II GTNNAC 6 cut(s) 235, 460, 511, 601, 1165, 1183
Hpy188I TCNGA 6 cut(s) 214, 541, 954, 1131, 1191, 1423
Hpy188III TCNNGA 4 cut(s) 204, 592, 726, 895
Hpy8I GTNNAC 6 cut(s) 235, 460, 511, 601, 1165, 1183
HpyAV CCTTC 1 cut(s) 1272
HpyCH4III ACNGT 3 cut(s) 508, 680, 1109
HpyCH4IV ACGT 4 cut(s) 435, 456, 642, 1433
HpyF10VI GCNNNNNNNGC 7 cut(s) 173, 534, 569, 572, 1293, 1413, 1472
HpyF3I CTNAG 4 cut(s) 351, 514, 936, 1348
HpySE526I ACGT 4 cut(s) 435, 456, 642, 1433
Hsp92II CATG 7 cut(s) 192, 318, 346, 493, 762, 1084, 1207
HspAI GCGC 3 cut(s) 171, 340, 1446
Kpn2I TCCGGA 1 cut(s) 591
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 1250, 1399
LmnI GCTCC 7 cut(s) 165, 320, 752, 815, 1355, 1423, 1479
Lsp1109I GCAGC 7 cut(s) 73, 160, 550, 609, 729, 742, 1072
LweI GCATC 2 cut(s) 33, 636
MaeI CTAG 3 cut(s) 83, 411, 1089
MaeII ACGT 4 cut(s) 435, 456, 642, 1433
MaeIII GTNAC 6 cut(s) 354, 446, 1025, 1039, 1112, 1123
MalI GATC 2 cut(s) 1252, 1401
MboI GATC 2 cut(s) 1250, 1399
MboII GAAGA 7 cut(s) 19, 124, 127, 497, 966, 1204, 1309
MhlI GDGCHC 6 cut(s) 325, 350, 539, 892, 1005, 1461
MluCI AATT 8 cut(s) 100, 199, 650, 657, 796, 854, 941, 1236
MlyI GAGTC 2 cut(s) 201, 423
MmeI TCCRAC 4 cut(s) 932, 1333, 1333, 1345
MreI CGCCGGCG 1 cut(s) 167
MroI TCCGGA 1 cut(s) 591
MroNI GCCGGC 1 cut(s) 167
MseI TTAA 3 cut(s) 20, 239, 1139
MslI CAYNNNNRTG 2 cut(s) 305, 1130
MspA1I CMGCKG 2 cut(s) 563, 843
MspCI CTTAAG 1 cut(s) 1138
MspI CCGG 5 cut(s) 168, 379, 423, 533, 592
MspR9I CCNGG 6 cut(s) 116, 140, 423, 534, 637, 1020
Mva1269I GAATGC 2 cut(s) 293, 1466
MvaI CCWGG 4 cut(s) 116, 140, 637, 1020
MvnI CGCG 2 cut(s) 369, 1404
MwoI GCNNNNNNNGC 7 cut(s) 173, 534, 569, 572, 1293, 1413, 1472
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 2 cut(s) 423, 534
NdeI CATATG 1 cut(s) 229
NdeII GATC 2 cut(s) 1250, 1399
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 7 cut(s) 192, 318, 346, 493, 762, 1084, 1207
NlaIV GGNNCC 7 cut(s) 161, 348, 817, 1219, 1357, 1425, 1459
NmuCI GTSAC 3 cut(s) 446, 1039, 1123
NotI GCGGCCGC 1 cut(s) 573
PctI GAATGC 2 cut(s) 293, 1466
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 3 cut(s) 683, 824, 1389
PflFI GACNNNGTC 1 cut(s) 269
PleI GAGTC 2 cut(s) 201, 423
PpsI GAGTC 2 cut(s) 201, 423
PshAI GACNNNNGTC 1 cut(s) 148
PsiI TTATAA 2 cut(s) 401, 803
Psp6I CCWGG 4 cut(s) 114, 138, 635, 1018
PspEI GGTNACC 2 cut(s) 446, 1112
PspFI CCCAGC 2 cut(s) 177, 843
PspGI CCWGG 4 cut(s) 114, 138, 635, 1018
PspN4I GGNNCC 7 cut(s) 161, 348, 817, 1219, 1357, 1425, 1459
PspOMI GGGCCC 2 cut(s) 346, 1457
PstI CTGCAG 2 cut(s) 91, 700
PstNI CAGNNNCTG 1 cut(s) 563
PsyI GACNNNGTC 1 cut(s) 269
PvuII CAGCTG 1 cut(s) 843
RsaI GTAC 5 cut(s) 512, 665, 982, 1079, 1436
RsaNI GTAC 5 cut(s) 511, 664, 981, 1078, 1435
RseI CAYNNNNRTG 2 cut(s) 305, 1130
SaqAI TTAA 3 cut(s) 20, 239, 1139
Sau3AI GATC 2 cut(s) 1250, 1399
ScaI AGTACT 1 cut(s) 982
SchI GAGTC 2 cut(s) 201, 423
ScrFI CCNGG 6 cut(s) 116, 140, 423, 534, 637, 1020
SduI GDGCHC 6 cut(s) 325, 350, 539, 892, 1005, 1461
SfaNI GCATC 2 cut(s) 33, 636
SfcI CTRYAG 3 cut(s) 87, 696, 1483
SgrAI CRCCGGYG 1 cut(s) 167
SinI GGWCC 2 cut(s) 195, 1370
SmiMI CAYNNNNRTG 2 cut(s) 305, 1130
SmlI CTYRAG 1 cut(s) 1138
SmoI CTYRAG 1 cut(s) 1138
Sse9I AATT 8 cut(s) 100, 199, 650, 657, 796, 854, 941, 1236
SsiI CCGC 6 cut(s) 165, 367, 561, 572, 576, 1404
SspI AATATT 1 cut(s) 611
SspMI CTAG 3 cut(s) 83, 411, 1089
StyD4I CCNGG 6 cut(s) 114, 138, 421, 532, 635, 1018
StyI CCWWGG 3 cut(s) 688, 810, 1461
TaaI ACNGT 3 cut(s) 508, 680, 1109
TaiI ACGT 4 cut(s) 438, 459, 645, 1436
TasI AATT 8 cut(s) 100, 199, 650, 657, 796, 854, 941, 1236
TatI WGTACW 3 cut(s) 510, 980, 1077
TauI GCSGC 4 cut(s) 563, 575, 578, 1407
TfiI GAWTC 3 cut(s) 683, 824, 1389
Tru1I TTAA 3 cut(s) 20, 239, 1139
Tru9I TTAA 3 cut(s) 20, 239, 1139
TscAI CASTG 2 cut(s) 685, 826
TseFI GTSAC 3 cut(s) 446, 1039, 1123
TseI GCWGC 7 cut(s) 86, 173, 563, 622, 742, 755, 1085
Tsp45I GTSAC 3 cut(s) 446, 1039, 1123
TspDTI ATGAA 3 cut(s) 19, 795, 1254
TspGWI ACGGA 1 cut(s) 845
TspRI CASTG 2 cut(s) 685, 826
Tth111I GACNNNGTC 1 cut(s) 269
Vha464I CTTAAG 1 cut(s) 1138
VpaK11BI GGWCC 2 cut(s) 195, 1370
XapI RAATTY 2 cut(s) 854, 1236
XmiI GTMKAC 1 cut(s) 459
XspI CTAG 3 cut(s) 83, 411, 1089
ZrmI AGTACT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.