pycom10g02920

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009372
Physical Location & Seq
Reverse (-)
3236133 .. 3246844
10712 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2259 bp
ATGCAACTGGTTCAAATTCCACAAAACCTCCTCTCCAAATTCCAATCCGCCTTCCGCTCATTTCACACACCACAACGCTCACTTTCCCACCATTCCAGTTCCTCCTCCACTTCTTCGCTTCCCTTCCAGAAAATGATTCTCCGCCTCCCCTCGCCTTCTCTCCCCTCCTCCTTCCTTTTCCCTTCCGCACCAAACCCCTTCCGGCGGTTCTCTTCATCCTCTTTCCCCCGCCCCCGCCATCGTCTCTCCATTCTGCGATGCGCCGCCGGAAACGACGTCGTCGAACTCGACGCCTTCACCAAGAAGTCGGGCTACCTCTTCGAGCTCAGCGCCCCCGAGGCCAACTCCATAGCCGACTACGATATCTCCAAAATCGGCGCCATTTACCGGCGGAAGCCTCTGATTCTTCTCCGCCGACTCTCTCAGATTGCCTTCACTTTCGGCAAGTGGTTCGCGGTTCGCTACATTGATAACCTGACGGAGCGCTCTGACCGAATGTTCGAGCTTAGAGCTGCCGAGCTTCGGAATATTCTGGTAGAACTCGGACCGGCATATGTCAAAATTGCTCAGGCCATCTCATCTCGACCAGACTTGATACCACCATCATACTTGGATGAGCTCTCACTGCTGCAAGATCGAATATCTCCATTTTTTACGGAAGTTGCTATAAACACAATAGAACAAGAACTTGGTTTACCGATAGAAGTGCTTTTCTCAGAGATCTCACCGGAACCTGTAGCTGCTGCTTCCCTTGGACAGGTTTATCAAGCCAGGCTTCGCCGTACGGGACAGCTTGTTGCAGTAAAGGTGCAAAGGCCAGGAGTACAAGCTGCTATTTCCTTAGACATATTGATCTTGCGTTTTCTAGCTGGTCTACTTAGGAGAATAAGAAAATTAAACACTGATCTTCAGGCAGTTGTTGATGAATGGGCATCAAGTCTTTTTCGGGAGATGGACTACAGAACTGAAGCAAATAATGGTATCAGATTCAGACAGCTATACGGTGGTATACCCGATGTTTTGGTTCCGGAAATGTATCTGGATTACACAACTCGCAGGGTTCTTGTGATGGAATGGGTGGAGGGGCAGAAGCTGTCAGAAGTGAATGATCTTTACTTGGTTGAGGTAGGGACGTACTGCTCATTCAATCAACTTCTGGAATGTGGATTCTACCATGCAGATCCACACCCTGGAAATCTTCTTCGTACATATGATGGGAAACTTGCTTATTTAGATTTTGGTATGATGGGTGAATTTAATCAAGAACTCCGCATTGGATTTATTGAAGCTTCTCTCCATCTTGTAAACCGTGATTTTGGTGCATTGGCTAAGGACTTTGTTACTCTTGGATTTATTCCACCAACTGCAGACAAGGAAGCTGTTACAAAGGCTTTAACAGGTGTCTTTCAGAATGCTGTTGCCAAAGGGGTGCGCAATATTAGCTTTGGAGACCTATTAGGAGATTTAGGAGCCACCATGTACAAGTTCAAATTTCGTATACCTTCTTATTTTTCTCTTGTCATTCGAAGCCTTGCCGTATTGGAAGGTGTTGCCATTGGCTTCAACCCAGATTACAAAGTTTTAGGTAGCACATACCCATGGATTGCTCGAAAAGTATTAACAGACAGCTCAATAGAGCTCAAGTCCTCCCTGCAGGCCCTTCTTTATGAGGATGGCGTTTTCAATATCGACCGTCTAGAGTCTTTACTTTCTGAGGCTCTCCGTGCTAGAACAGAGAAGGTCATGTCAAGAAAGCAAGAAGATGATGCTAATTCTAGGGTAGTTGTCAAACAAGTCCTTTCTTTTATGTTGGCAGAGAAGGGTGCATTTGTGAGGGATCTACTTCTTCAAGAATTTGTTAAGGGATTGGATGCACTTGGGCTGGCAACCTTGGATTCTATTATGACAAGTATACCTTTCGCTGCTACCTTGTCCATCTCAACAATGACCAATGAAGACAAAATCAACTTAAGAACACTCCGTCGCCTTCTGCGATTGCTGTCAGGGTTGCAAAGAAATGAAAACTCCAAACCATTTATCAGAGACGATACCCCATACAAGAATCAGCAAACGAACTCGGATGAAGCAGCATTAGTTTTCAACCAACTTGCATCTGTTCAAGAAATTCTACCTCTCCTTTCTGTTCTTCCTGAGCTCCCACCAGAATCACAACAGCAGTTACTTAATCTCCCAGCTGATCTTACAGGACGATTAATTTCCCGTGCTGCTGCAAGGACAATCCGAAGGATTGTTTTATGA

Protein Analysis

753

Amino Acids

84.52

Weight (kDa)

8.98

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 210 - 444 4.2e-67 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
Acc16I TGCGCA 1 cut(s) 1433
AccB1I GGYRCC 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 1190
AccBSI CCGCTC 1 cut(s) 57
AccI GTMKAC 4 cut(s) 874, 1009, 1498, 1912
AccII CGCG 1 cut(s) 455
AccIII TCCGGA 1 cut(s) 1027
AclWI GGATC 2 cut(s) 1175, 1845
AcsI RAATTY 6 cut(s) 15, 38, 1253, 1490, 1853, 2124
AcuI CTGAAG 2 cut(s) 893, 987
AcyI GRCGYC 3 cut(s) 276, 291, 378
AfaI GTAC 5 cut(s) 784, 825, 1136, 1207, 1481
AfeI AGCGCT 1 cut(s) 485
AfiI CCNNNNNNNGG 5 cut(s) 204, 387, 522, 757, 1190
AflII CTTAAG 1 cut(s) 1969
AgsI TTSAA 9 cut(s) 14, 1147, 1286, 1489, 1564, 1684, 1850, 2101, 2120
AjnI CCWGG 3 cut(s) 770, 817, 1189
AjuI GAANNNNNNNTTGG 4 cut(s) 672, 704, 1257, 1289
Alw21I GWGCWC 4 cut(s) 327, 621, 1641, 2157
Alw26I GTCTC 3 cut(s) 248, 1443, 2037
AlwI GGATC 2 cut(s) 1175, 1845
AlwNI CAGNNNCTG 1 cut(s) 1093
Ama87I CYCGRG 1 cut(s) 335
Aor13HI TCCGGA 1 cut(s) 1027
Aor51HI AGCGCT 1 cut(s) 485
AoxI GGCC 4 cut(s) 339, 570, 815, 1656
ApeKI GCWGC 9 cut(s) 512, 628, 740, 743, 830, 1922, 2087, 2225, 2228
ApoI RAATTY 6 cut(s) 15, 38, 1253, 1490, 1853, 2124
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
AseI ATTAAT 1 cut(s) 2213
AspLEI GCGC 5 cut(s) 263, 332, 380, 486, 1434
AspS9I GGNCC 2 cut(s) 545, 1657
AsuHPI GGTGA 3 cut(s) 289, 717, 1262
AsuII TTCGAA 1 cut(s) 1525
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 1 cut(s) 545
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 4 cut(s) 327, 621, 1641, 2157
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbsI GAAGAC 1 cut(s) 1962
Bbv12I GWGCWC 4 cut(s) 327, 621, 1641, 2157
BbvI GCAGC 9 cut(s) 499, 615, 727, 730, 817, 1909, 2099, 2212, 2215
BceAI ACGGC 2 cut(s) 765, 1520
BcgI CGANNNNNNTGC 4 cut(s) 433, 467, 688, 722
BciT130I CCWGG 3 cut(s) 772, 819, 1191
BcoDI GTCTC 3 cut(s) 248, 1443, 2037
BfaI CTAG 4 cut(s) 866, 1697, 1728, 1776
BfmI CTRYAG 4 cut(s) 735, 958, 1365, 1652
BfoI RGCGCY 3 cut(s) 333, 381, 487
BfrI CTTAAG 1 cut(s) 1969
BglI GCCNNNNNGGC 1 cut(s) 338
BglII AGATCT 1 cut(s) 720
BlpI GCTNAGC 1 cut(s) 326
Bme1390I CCNGG 3 cut(s) 772, 819, 1191
Bme18I GGWCC 1 cut(s) 545
BmeT110I CYCGRG 1 cut(s) 335
BmgT120I GGNCC 2 cut(s) 545, 1657
BmiI GGNNCC 4 cut(s) 379, 732, 1026, 1471
BmrFI CCNGG 3 cut(s) 772, 819, 1191
BmsI GCATC 5 cut(s) 248, 941, 1756, 1861, 2120
BpiI GAAGAC 1 cut(s) 1962
BplI GAGNNNNNCTC 2 cut(s) 329, 361
Bpu10I CCTNAGC 3 cut(s) 567, 1329, 2151
Bpu1102I GCTNAGC 1 cut(s) 326
Bpu14I TTCGAA 1 cut(s) 1525
BpuEI CTTGAG 1 cut(s) 1625
BsaHI GRCGYC 3 cut(s) 276, 291, 378
BsaI GGTCTC 1 cut(s) 1443
BsaJI CCNNGG 5 cut(s) 336, 751, 1189, 1598, 1890
BsaWI WCCGGW 2 cut(s) 727, 1027
BsaXI ACNNNNNCTCC 8 cut(s) 12, 17, 42, 47, 350, 380, 2172, 2202
Bsc4I CCNNNNNNNGG 5 cut(s) 204, 387, 522, 757, 1190
Bse118I RCCGGY 2 cut(s) 387, 547
Bse1I ACTGG 2 cut(s) 12, 96
BseAI TCCGGA 1 cut(s) 1027
BseBI CCWGG 3 cut(s) 772, 819, 1191
BseDI CCNNGG 5 cut(s) 336, 751, 1189, 1598, 1890
BseGI GGATG 5 cut(s) 215, 619, 1678, 1876, 2086
BseLI CCNNNNNNNGG 5 cut(s) 204, 387, 522, 757, 1190
BseMII CTCAG 6 cut(s) 340, 437, 581, 729, 1704, 2142
BseNI ACTGG 2 cut(s) 12, 96
BseRI GAGGAG 3 cut(s) 20, 94, 157
BseXI GCAGC 9 cut(s) 499, 615, 727, 730, 817, 1909, 2099, 2212, 2215
BseYI CCCAGC 1 cut(s) 2191
Bsh1236I CGCG 1 cut(s) 455
Bsh1285I CGRYCG 1 cut(s) 1693
BshFI GGCC 4 cut(s) 341, 572, 817, 1658
BshNI GGYRCC 1 cut(s) 377
BsiEI CGRYCG 1 cut(s) 1693
BsiHKAI GWGCWC 4 cut(s) 327, 621, 1641, 2157
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 6 cut(s) 202, 267, 388, 548, 728, 1028
BsiWI CGTACG 1 cut(s) 782
BslFI GGGAC 2 cut(s) 801, 1144
BslI CCNNNNNNNGG 5 cut(s) 204, 387, 522, 757, 1190
BsmAI GTCTC 3 cut(s) 248, 1443, 2037
BsmBI CGTCTC 2 cut(s) 248, 2037
BsmFI GGGAC 2 cut(s) 801, 1144
BsmI GAATGC 1 cut(s) 1417
BsnI GGCC 4 cut(s) 341, 572, 817, 1658
Bso31I GGTCTC 1 cut(s) 1443
BsoBI CYCGRG 1 cut(s) 335
Bsp119I TTCGAA 1 cut(s) 1525
Bsp1286I GDGCHC 4 cut(s) 327, 621, 1641, 2157
Bsp13I TCCGGA 1 cut(s) 1027
Bsp1407I TGTACA 1 cut(s) 1479
Bsp143I GATC 8 cut(s) 634, 720, 852, 904, 1108, 1180, 1837, 2197
Bsp1720I GCTNAGC 1 cut(s) 326
Bsp19I CCATGG 1 cut(s) 1598
BspANI GGCC 4 cut(s) 341, 572, 817, 1658
BspCNI CTCAG 6 cut(s) 339, 436, 580, 728, 1705, 2143
BspEI TCCGGA 1 cut(s) 1027
BspFNI CGCG 1 cut(s) 455
BspLI GGNNCC 4 cut(s) 379, 732, 1026, 1471
BspMAI CTGCAG 2 cut(s) 1369, 1656
BspPI GGATC 2 cut(s) 1175, 1845
BspT104I TTCGAA 1 cut(s) 1525
BspT107I GGYRCC 1 cut(s) 377
BspTI CTTAAG 1 cut(s) 1969
BspTNI GGTCTC 1 cut(s) 1443
BsrBI CCGCTC 1 cut(s) 57
BsrFI RCCGGY 2 cut(s) 387, 547
BsrGI TGTACA 1 cut(s) 1479
BsrI ACTGG 2 cut(s) 12, 96
BssAI RCCGGY 2 cut(s) 387, 547
BssECI CCNNGG 5 cut(s) 336, 751, 1189, 1598, 1890
BssMI GATC 8 cut(s) 634, 720, 852, 904, 1108, 1180, 1837, 2197
BssNAI GTATAC 3 cut(s) 1010, 1499, 1913
BssNI GRCGYC 3 cut(s) 276, 291, 378
BssT1I CCWWGG 3 cut(s) 751, 1598, 1890
Bst1107I GTATAC 3 cut(s) 1010, 1499, 1913
Bst2UI CCWGG 3 cut(s) 772, 819, 1191
Bst4CI ACNGT 3 cut(s) 1004, 1310, 1694
Bst6I CTCTTC 2 cut(s) 217, 323
BstACI GRCGYC 3 cut(s) 276, 291, 378
BstAFI CTTAAG 1 cut(s) 1969
BstAUI TGTACA 1 cut(s) 1479
BstBI TTCGAA 1 cut(s) 1525
BstC8I GCNNGC 2 cut(s) 1656, 1884
BstDSI CCRYGG 1 cut(s) 1598
BstENI CCTNNNNNAGG 1 cut(s) 755
BstF5I GGATG 5 cut(s) 215, 619, 1678, 1876, 2086
BstFNI CGCG 1 cut(s) 455
BstH2I RGCGCY 3 cut(s) 333, 381, 487
BstHHI GCGC 5 cut(s) 263, 332, 380, 486, 1434
BstKTI GATC 8 cut(s) 637, 723, 855, 907, 1111, 1183, 1840, 2200
BstMAI GTCTC 3 cut(s) 248, 1443, 2037
BstMBI GATC 8 cut(s) 634, 720, 852, 904, 1108, 1180, 1837, 2197
BstMCI CGRYCG 1 cut(s) 1693
BstMWI GCNNNNNNNGC 4 cut(s) 338, 625, 1440, 1724
BstNI CCWGG 3 cut(s) 772, 819, 1191
BstSCI CCNGG 3 cut(s) 770, 817, 1189
BstSFI CTRYAG 4 cut(s) 735, 958, 1365, 1652
BstUI CGCG 1 cut(s) 455
BstV1I GCAGC 9 cut(s) 499, 615, 727, 730, 817, 1909, 2099, 2212, 2215
BstV2I GAAGAC 1 cut(s) 1962
BstX2I RGATCY 3 cut(s) 720, 1180, 1837
BstYI RGATCY 3 cut(s) 720, 1180, 1837
BstZ17I GTATAC 3 cut(s) 1010, 1499, 1913
BsuRI GGCC 4 cut(s) 341, 572, 817, 1658
BtgI CCRYGG 1 cut(s) 1598
BtgZI GCGATG 1 cut(s) 271
BtsCI GGATG 5 cut(s) 215, 619, 1678, 1876, 2086
BtsI GCAGTG 1 cut(s) 623
BtsIMutI CAGTG 2 cut(s) 623, 900
Cac8I GCNNGC 2 cut(s) 1656, 1884
CaiI CAGNNNCTG 1 cut(s) 1093
CfoI GCGC 5 cut(s) 263, 332, 380, 486, 1434
Cfr10I RCCGGY 2 cut(s) 387, 547
Cfr13I GGNCC 2 cut(s) 545, 1657
CpoI CGGWCCG 1 cut(s) 545
CseI GACGC 1 cut(s) 299
Csp6I GTAC 5 cut(s) 783, 824, 1135, 1206, 1480
CspI CGGWCCG 1 cut(s) 545
CviAII CATG 4 cut(s) 1175, 1477, 1599, 1744
CviQI GTAC 5 cut(s) 783, 824, 1135, 1206, 1480
DinI GGCGCC 1 cut(s) 379
DpnI GATC 8 cut(s) 636, 722, 854, 906, 1110, 1182, 1839, 2199
DpnII GATC 8 cut(s) 634, 720, 852, 904, 1108, 1180, 1837, 2197
Eam1104I CTCTTC 2 cut(s) 217, 323
EarI CTCTTC 2 cut(s) 217, 323
EciI GGCGGA 4 cut(s) 37, 131, 401, 406
Ecl136II GAGCTC 4 cut(s) 325, 619, 1639, 2155
Eco130I CCWWGG 3 cut(s) 751, 1598, 1890
Eco24I GRGCYC 4 cut(s) 327, 621, 1641, 2157
Eco31I GGTCTC 1 cut(s) 1443
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 1 cut(s) 545
Eco47III AGCGCT 1 cut(s) 485
Eco53kI GAGCTC 4 cut(s) 325, 619, 1639, 2155
Eco57I CTGAAG 2 cut(s) 893, 987
Eco88I CYCGRG 1 cut(s) 335
EcoICRI GAGCTC 4 cut(s) 325, 619, 1639, 2155
EcoNI CCTNNNNNAGG 1 cut(s) 755
EcoO109I RGGNCCY 1 cut(s) 1657
EcoRII CCWGG 3 cut(s) 770, 817, 1189
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 3 cut(s) 751, 1598, 1890
EcoT38I GRGCYC 4 cut(s) 327, 621, 1641, 2157
EgeI GGCGCC 1 cut(s) 379
EheI GGCGCC 1 cut(s) 379
ErhI CCWWGG 3 cut(s) 751, 1598, 1890
Esp3I CGTCTC 2 cut(s) 248, 2037
FaeI CATG 4 cut(s) 1178, 1480, 1602, 1747
FalI AAGNNNNNCTT 4 cut(s) 759, 791, 1900, 1932
FaqI GGGAC 2 cut(s) 801, 1144
FatI CATG 4 cut(s) 1174, 1476, 1598, 1743
FauI CCCGC 2 cut(s) 236, 242
FauNDI CATATG 2 cut(s) 553, 1210
FblI GTMKAC 4 cut(s) 874, 1009, 1498, 1912
FokI GGATG 5 cut(s) 202, 626, 1685, 1883, 2093
FriOI GRGCYC 4 cut(s) 327, 621, 1641, 2157
FspBI CTAG 4 cut(s) 866, 1697, 1728, 1776
FspI TGCGCA 1 cut(s) 1433
GlaI GCGC 5 cut(s) 262, 331, 379, 485, 1433
GsaI CCCAGC 1 cut(s) 2195
HaeII RGCGCY 3 cut(s) 333, 381, 487
HaeIII GGCC 4 cut(s) 341, 572, 817, 1658
HapII CCGG 6 cut(s) 202, 267, 388, 548, 728, 1028
HgaI GACGC 1 cut(s) 299
HhaI GCGC 5 cut(s) 263, 332, 380, 486, 1434
Hin1I GRCGYC 3 cut(s) 276, 291, 378
Hin1II CATG 4 cut(s) 1178, 1480, 1602, 1747
Hin6I GCGC 5 cut(s) 261, 330, 378, 484, 1432
HinP1I GCGC 5 cut(s) 261, 330, 378, 484, 1432
HindIII AAGCTT 1 cut(s) 1287
HinfI GANTC 9 cut(s) 136, 403, 417, 987, 1167, 1700, 1895, 2062, 2165
HpaII CCGG 6 cut(s) 202, 267, 388, 548, 728, 1028
HphI GGTGA 3 cut(s) 289, 717, 1262
Hpy166II GTNNAC 6 cut(s) 695, 875, 1010, 1306, 1499, 1913
Hpy8I GTNNAC 6 cut(s) 695, 875, 1010, 1306, 1499, 1913
Hpy99I CGWCG 5 cut(s) 278, 281, 284, 293, 1986
HpyCH4III ACNGT 3 cut(s) 1004, 1310, 1694
HpyCH4IV ACGT 2 cut(s) 276, 1133
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 625, 1440, 1724
HpySE526I ACGT 2 cut(s) 276, 1133
Hsp92I GRCGYC 3 cut(s) 276, 291, 378
Hsp92II CATG 4 cut(s) 1178, 1480, 1602, 1747
HspAI GCGC 5 cut(s) 261, 330, 378, 484, 1432
KasI GGCGCC 1 cut(s) 377
Kpn2I TCCGGA 1 cut(s) 1027
Kzo9I GATC 8 cut(s) 634, 720, 852, 904, 1108, 1180, 1837, 2197
LmnI GCTCC 3 cut(s) 481, 1469, 2160
Lsp1109I GCAGC 9 cut(s) 499, 615, 727, 730, 817, 1909, 2099, 2212, 2215
LweI GCATC 5 cut(s) 248, 941, 1756, 1861, 2120
MaeI CTAG 4 cut(s) 866, 1697, 1728, 1776
MaeII ACGT 2 cut(s) 276, 1133
MaeIII GTNAC 3 cut(s) 1339, 1381, 2178
MalI GATC 8 cut(s) 636, 722, 854, 906, 1110, 1182, 1839, 2199
MbiI CCGCTC 1 cut(s) 57
MboI GATC 8 cut(s) 634, 720, 852, 904, 1108, 1180, 1837, 2197
MflI RGATCY 3 cut(s) 720, 1180, 1837
MhlI GDGCHC 4 cut(s) 327, 621, 1641, 2157
Mly113I GGCGCC 1 cut(s) 378
MlyI GAGTC 2 cut(s) 411, 1709
MroI TCCGGA 1 cut(s) 1027
MseI TTAA 8 cut(s) 896, 1257, 1394, 1619, 1860, 1970, 2184, 2213
MslI CAYNNNNRTG 1 cut(s) 1597
MspA1I CMGCKG 1 cut(s) 2195
MspCI CTTAAG 1 cut(s) 1969
MspI CCGG 6 cut(s) 202, 267, 388, 548, 728, 1028
MspR9I CCNGG 3 cut(s) 772, 819, 1191
Mva1269I GAATGC 1 cut(s) 1417
MvaI CCWGG 3 cut(s) 772, 819, 1191
MvnI CGCG 1 cut(s) 455
MwoI GCNNNNNNNGC 4 cut(s) 338, 625, 1440, 1724
NarI GGCGCC 1 cut(s) 378
NcoI CCATGG 1 cut(s) 1598
NdeI CATATG 2 cut(s) 553, 1210
NdeII GATC 8 cut(s) 634, 720, 852, 904, 1108, 1180, 1837, 2197
NlaIII CATG 4 cut(s) 1178, 1480, 1602, 1747
NlaIV GGNNCC 4 cut(s) 379, 732, 1026, 1471
NmeAIII GCCGAG 1 cut(s) 541
NsbI TGCGCA 1 cut(s) 1433
NspV TTCGAA 1 cut(s) 1525
PcsI WCGNNNNNNNCGW 3 cut(s) 279, 285, 1990
PctI GAATGC 1 cut(s) 1417
PfeI GAWTC 7 cut(s) 136, 403, 987, 1167, 1895, 2062, 2165
Pfl23II CGTACG 1 cut(s) 782
PflFI GACNNNGTC 1 cut(s) 278
PflMI CCANNNNNTGG 1 cut(s) 1190
PleI GAGTC 2 cut(s) 411, 1708
PluTI GGCGCC 1 cut(s) 381
PpsI GAGTC 2 cut(s) 411, 1708
PshBI ATTAAT 1 cut(s) 2213
Psp124BI GAGCTC 4 cut(s) 327, 621, 1641, 2157
Psp6I CCWGG 3 cut(s) 770, 817, 1189
PspFI CCCAGC 1 cut(s) 2191
PspGI CCWGG 3 cut(s) 770, 817, 1189
PspLI CGTACG 1 cut(s) 782
PspN4I GGNNCC 4 cut(s) 379, 732, 1026, 1471
PspPI GGNCC 2 cut(s) 545, 1657
PsrI GAACNNNNNNTAC 2 cut(s) 678, 710
PstI CTGCAG 2 cut(s) 1369, 1656
PstNI CAGNNNCTG 1 cut(s) 1093
PsuI RGATCY 3 cut(s) 720, 1180, 1837
PsyI GACNNNGTC 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 2195
RsaI GTAC 5 cut(s) 784, 825, 1136, 1207, 1481
RsaNI GTAC 5 cut(s) 783, 824, 1135, 1206, 1480
RseI CAYNNNNRTG 1 cut(s) 1597
Rsr2I CGGWCCG 1 cut(s) 545
RsrII CGGWCCG 1 cut(s) 545
SacI GAGCTC 4 cut(s) 327, 621, 1641, 2157
SaqAI TTAA 8 cut(s) 896, 1257, 1394, 1619, 1860, 1970, 2184, 2213
Sau3AI GATC 8 cut(s) 634, 720, 852, 904, 1108, 1180, 1837, 2197
Sau96I GGNCC 2 cut(s) 545, 1657
SbfI CCTGCAGG 1 cut(s) 1656
SchI GAGTC 2 cut(s) 411, 1709
ScrFI CCNGG 3 cut(s) 772, 819, 1191
SdaI CCTGCAGG 1 cut(s) 1656
SduI GDGCHC 4 cut(s) 327, 621, 1641, 2157
SfaNI GCATC 5 cut(s) 248, 941, 1756, 1861, 2120
SfcI CTRYAG 4 cut(s) 735, 958, 1365, 1652
SfoI GGCGCC 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 1525
SinI GGWCC 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 1597
SmlI CTYRAG 2 cut(s) 1640, 1969
SmoI CTYRAG 2 cut(s) 1640, 1969
Sse8387I CCTGCAGG 1 cut(s) 1656
SspDI GGCGCC 1 cut(s) 377
SspI AATATT 2 cut(s) 529, 1438
SspMI CTAG 4 cut(s) 866, 1697, 1728, 1776
SstI GAGCTC 4 cut(s) 327, 621, 1641, 2157
StyD4I CCNGG 3 cut(s) 770, 817, 1189
StyI CCWWGG 3 cut(s) 751, 1598, 1890
TaaI ACNGT 3 cut(s) 1004, 1310, 1694
TaiI ACGT 2 cut(s) 279, 1136
TaqI TCGA 9 cut(s) 282, 288, 321, 501, 583, 637, 1525, 1609, 1689
TaqII GACCGA 1 cut(s) 507
TatI WGTACW 2 cut(s) 823, 1479
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 7 cut(s) 136, 403, 987, 1167, 1895, 2062, 2165
Tru1I TTAA 8 cut(s) 896, 1257, 1394, 1619, 1860, 1970, 2184, 2213
Tru9I TTAA 8 cut(s) 896, 1257, 1394, 1619, 1860, 1970, 2184, 2213
TscAI CASTG 2 cut(s) 630, 907
TseI GCWGC 9 cut(s) 512, 628, 740, 743, 830, 1922, 2087, 2225, 2228
TspDTI ATGAA 5 cut(s) 204, 939, 1968, 2034, 2097
TspGWI ACGGA 4 cut(s) 494, 671, 1712, 1970
TspRI CASTG 2 cut(s) 630, 907
Tth111I GACNNNGTC 1 cut(s) 278
Van91I CCANNNNNTGG 1 cut(s) 1190
Vha464I CTTAAG 1 cut(s) 1969
VpaK11BI GGWCC 1 cut(s) 545
VspI ATTAAT 1 cut(s) 2213
XagI CCTNNNNNAGG 1 cut(s) 755
XapI RAATTY 6 cut(s) 15, 38, 1253, 1490, 1853, 2124
XbaI TCTAGA 1 cut(s) 1696
XmiI GTMKAC 4 cut(s) 874, 1009, 1498, 1912
XspI CTAG 4 cut(s) 866, 1697, 1728, 1776
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.