pycom10g04110

DnaJ molecular chaperone homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009201
Physical Location & Seq
Reverse (-)
4382893 .. 4384601
1709 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1566 bp
ATGGAGTTCAATTTCAACACCAACAGGGCGGAAGCGGAGCGGTGGCTGAGCACCGCCGTGAAGCTACTGTCGGCGCATGACTTGCAGGGAACCAAGACCTTCGCGATCCGAGCCCGAGAGACCGACCCCAGGCTCGAGGCCACCGAGCAGATAATCGCCGTTGCCGACACTCTCCTCGCCGCTGAATCCCAGATCAACAACCAAAACCAGCAGCCCGACTGGTACGCTATTCTCCAACTCGCCCAGTACACTCAGAACCTCGAAATCGTCGCGACTCAGTACCGCCGACTCGCTCTTCTGCTGAACCCCCACCGGAATCGCTTCCCTTACGCCGATCATGCCTTCCGGCTGGTCTCCGAGGCCTGGAACGTCCTCTCTAACCCCACTAAGAAGGCCATGTACGACCACGAGTTGAGCATGTTCAACCGGTTCGACTCGCCCCCATCCGGGTCGACTCAGCTGTTCGAGAGGCAATTCTTGCAAATGCATCATGTTCAGCAACAGCCGCCGCAGCCACCACAGCCACCACCACCACAGCCACTTCTATTTCATCCCCAACCGTCACAGCTACTACAATTCCAGCCTCAACCGCCACCGCAGCCGCAACCACAACCACAACCAGAAGTGCAGAGACCACAGCCGCAATGGAATTTTCAACAAAAGCGACAACAACCACCGCCAGAGCAGCAACATCAGCAGCAGCACCATCAGCAACATCACCACGAACCTCCTCAGCAGCAGCAGCAGCAACAATATCAGGAACTTCCCCAGCTGTGGCAGCAGCTGCAGCAACAACAGCAGCAACAACAGCAGCAACAAGAAGTGAGTAAAAACCCTAGAAGTAAGGACGGAAGACCGTCGATGGAGGAGGAAAGGCCGATTCCGATTCCCATCAATGTGACCGAGCCTGCACCTCCGTCCTCTGAGTCAACTCCTCCGCCCTCCGAATCAACTGGCCCTGTTGAGTCAACTCCTCCTTCTGAGTCAGCTCCCCCTTCTGAGTCGACTCGGCCTCAAACAGTGTCCAAAACGGGGAGCTTCTGGACTGCATGTCCTTACTGTTACAACCTCTTCGAGTATCCTAGCGCTTACGAGGATTGCATGCTTCGGTGCCAGAATTGTAAGAGGGCATTCAATGCAATGGCGATAGCGGCACCGCCTCAGGCGGGGAAAGATGTGAACTTCTGCTGTTGGGGGTATTTCCCATTGGGGTTACTGGAGAATAGTAAGGATACAGGCGGATCAGCGGAGGTGTGGACACCATTTTCGACAATGTTTGCTTGCCCAATACAAGGGAAGAAGAATACCGCACGAGCCAAGATTGCTAATTCGGGGCCAAGGGTCTATTATGATGATGAGGAAGCGTTGCTTGATGTTTCGGATCCAAGTGAGGACTCTGATGATGATGATGAGTGGCAGAGGGTTAGGAGGAAGAAGAGAGTTGTAAAGGCAAAAGGTAAAGCTTCCACCGCTAAGGCTCCGGTGAGAGCTTCGGATAGATTAAGGAAGGGGAGTCAGAATGCTGGTGGGCAGGGGGGAAGAGCCTGCGGCTCAGACAATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

522

Amino Acids

59.24

Weight (kDa)

6.26

Isoelectric Point (pI)

72.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 73 - 135 3.3e-13 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1110, 1153
AccBSI CCGCTC 1 cut(s) 40
AccI GTMKAC 2 cut(s) 452, 1004
AccII CGCG 2 cut(s) 104, 272
AclWI GGATC 4 cut(s) 100, 1249, 1376, 1389
AcsI RAATTY 1 cut(s) 649
AfaI GTAC 4 cut(s) 224, 248, 281, 401
AfeI AGCGCT 1 cut(s) 1087
AfiI CCNNNNNNNGG 8 cut(s) 129, 363, 446, 447, 774, 1032, 1166, 1292
AgeI ACCGGT 1 cut(s) 426
AgsI TTSAA 5 cut(s) 10, 16, 424, 656, 1135
AhdI GACNNNNNGTC 1 cut(s) 1050
AjnI CCWGG 2 cut(s) 128, 362
AleI CACNNNNGTG 1 cut(s) 56
AluBI AGCT 9 cut(s) 64, 460, 568, 772, 784, 989, 1038, 1463, 1490
AluI AGCT 9 cut(s) 64, 460, 568, 772, 784, 989, 1038, 1463, 1490
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 3 cut(s) 113, 358, 625
AlwI GGATC 4 cut(s) 100, 1249, 1376, 1389
AlwNI CAGNNNCTG 1 cut(s) 784
Ama87I CYCGRG 2 cut(s) 114, 134
Aor51HI AGCGCT 1 cut(s) 1087
AoxI GGCC 7 cut(s) 138, 360, 393, 875, 955, 1010, 1334
ApoI RAATTY 1 cut(s) 649
ArsI GACNNNNNNTTYG 2 cut(s) 1249, 1281
AsiGI ACCGGT 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 320
AspLEI GCGC 2 cut(s) 76, 1088
AspS9I GGNCC 2 cut(s) 956, 1334
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 2 cut(s) 710, 1495
AvaI CYCGRG 2 cut(s) 114, 134
AxyI CCTNAGG 1 cut(s) 1161
BamHI GGATCC 1 cut(s) 1381
BanI GGYRCC 2 cut(s) 1110, 1153
BanII GRGCYC 1 cut(s) 115
BarI GAAGNNNNNNTAC 2 cut(s) 383, 415
BauI CACGAG 2 cut(s) 407, 1311
BbsI GAAGAC 1 cut(s) 859
Bbv12I GWGCWC 1 cut(s) 53
BbvCI CCTCAGC 1 cut(s) 732
BccI CCATC 4 cut(s) 451, 714, 856, 899
BceAI ACGGC 2 cut(s) 41, 143
BciT130I CCWGG 2 cut(s) 130, 364
BciVI GTATCC 2 cut(s) 1089, 1225
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 3 cut(s) 113, 358, 625
BfaI CTAG 2 cut(s) 837, 1083
BfmI CTRYAG 1 cut(s) 785
BfoI RGCGCY 1 cut(s) 1089
BfuI GTATCC 2 cut(s) 1089, 1225
BlpI GCTNAGC 1 cut(s) 47
Bme1390I CCNGG 3 cut(s) 130, 364, 448
BmeRI GACNNNNNGTC 1 cut(s) 1050
BmeT110I CYCGRG 2 cut(s) 114, 134
BmgT120I GGNCC 2 cut(s) 956, 1334
BmiI GGNNCC 6 cut(s) 91, 1112, 1155, 1335, 1383, 1479
BmrFI CCNGG 3 cut(s) 130, 364, 448
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 496
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 1 cut(s) 859
BpmI CTGGAG 1 cut(s) 1238
Bpu10I CCTNAGC 2 cut(s) 732, 1473
Bpu1102I GCTNAGC 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 448
BsaBI GATNNNNATC 1 cut(s) 890
BsaI GGTCTC 3 cut(s) 113, 358, 625
BsaJI CCNNGG 3 cut(s) 128, 357, 1337
BsaWI WCCGGW 3 cut(s) 312, 426, 1480
Bsc4I CCNNNNNNNGG 8 cut(s) 129, 363, 446, 447, 774, 1032, 1166, 1292
Bse118I RCCGGY 1 cut(s) 426
Bse1I ACTGG 4 cut(s) 224, 244, 958, 1221
Bse21I CCTNAGG 1 cut(s) 1161
Bse3DI GCAATG 2 cut(s) 650, 1146
Bse8I GATNNNNATC 1 cut(s) 890
BseBI CCWGG 2 cut(s) 130, 364
BseDI CCNNGG 3 cut(s) 128, 357, 1337
BseGI GGATG 2 cut(s) 443, 550
BseJI GATNNNNATC 1 cut(s) 890
BseLI CCNNNNNNNGG 8 cut(s) 129, 363, 446, 447, 774, 1032, 1166, 1292
BseMI GCAATG 2 cut(s) 650, 1146
BseNI ACTGG 4 cut(s) 224, 244, 958, 1221
BseRI GAGGAG 5 cut(s) 164, 720, 881, 924, 963
BseYI CCCAGC 1 cut(s) 768
BsgI GTGCAG 2 cut(s) 647, 894
Bsh1236I CGCG 2 cut(s) 104, 272
BshFI GGCC 7 cut(s) 140, 362, 395, 877, 957, 1012, 1336
BshNI GGYRCC 2 cut(s) 1110, 1153
BshTI ACCGGT 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 53
BsiHKCI CYCGRG 2 cut(s) 114, 134
BsiSI CCGG 5 cut(s) 313, 346, 427, 447, 1481
BslI CCNNNNNNNGG 8 cut(s) 129, 363, 446, 447, 774, 1032, 1166, 1292
BsmAI GTCTC 3 cut(s) 113, 358, 625
BsmI GAATGC 2 cut(s) 1130, 1525
BsnI GGCC 7 cut(s) 140, 362, 395, 877, 957, 1012, 1336
Bso31I GGTCTC 3 cut(s) 113, 358, 625
BsoBI CYCGRG 2 cut(s) 114, 134
Bsp1286I GDGCHC 2 cut(s) 53, 115
Bsp143I GATC 5 cut(s) 105, 192, 334, 1241, 1381
Bsp1720I GCTNAGC 1 cut(s) 47
Bsp68I TCGCGA 2 cut(s) 104, 272
BspANI GGCC 7 cut(s) 140, 362, 395, 877, 957, 1012, 1336
BspFNI CGCG 2 cut(s) 104, 272
BspLI GGNNCC 6 cut(s) 91, 1112, 1155, 1335, 1383, 1479
BspMAI CTGCAG 1 cut(s) 789
BspPI GGATC 4 cut(s) 100, 1249, 1376, 1389
BspQI GCTCTTC 2 cut(s) 300, 1534
BspT107I GGYRCC 2 cut(s) 1110, 1153
BspTNI GGTCTC 3 cut(s) 113, 358, 625
BsrBI CCGCTC 1 cut(s) 40
BsrDI GCAATG 2 cut(s) 650, 1146
BsrFI RCCGGY 1 cut(s) 426
BsrI ACTGG 4 cut(s) 224, 244, 958, 1221
BssAI RCCGGY 1 cut(s) 426
BssECI CCNNGG 3 cut(s) 128, 357, 1337
BssMI GATC 5 cut(s) 105, 192, 334, 1241, 1381
BssSI CACGAG 2 cut(s) 407, 1311
BssT1I CCWWGG 1 cut(s) 1337
Bst2BI CACGAG 2 cut(s) 407, 1311
Bst2UI CCWGG 2 cut(s) 130, 364
Bst4CI ACNGT 5 cut(s) 69, 561, 858, 1021, 1061
Bst6I CTCTTC 4 cut(s) 300, 1076, 1430, 1534
BstAPI GCANNNNNTGC 4 cut(s) 82, 478, 784, 1136
BstC8I GCNNGC 4 cut(s) 909, 1103, 1282, 1546
BstF5I GGATG 2 cut(s) 443, 550
BstFNI CGCG 2 cut(s) 104, 272
BstH2I RGCGCY 1 cut(s) 1089
BstHHI GCGC 2 cut(s) 76, 1088
BstKTI GATC 5 cut(s) 108, 195, 337, 1244, 1384
BstMAI GTCTC 3 cut(s) 113, 358, 625
BstMBI GATC 5 cut(s) 105, 192, 334, 1241, 1381
BstNI CCWGG 2 cut(s) 130, 364
BstNSI RCATGY 3 cut(s) 421, 1053, 1105
BstSCI CCNGG 3 cut(s) 128, 362, 446
BstSFI CTRYAG 1 cut(s) 785
BstUI CGCG 2 cut(s) 104, 272
BstV2I GAAGAC 1 cut(s) 859
BstX2I RGATCY 1 cut(s) 1381
BstYI RGATCY 1 cut(s) 1381
Bsu36I CCTNAGG 1 cut(s) 1161
BsuI GTATCC 2 cut(s) 1089, 1225
BsuRI GGCC 7 cut(s) 140, 362, 395, 877, 957, 1012, 1336
BtsCI GGATG 2 cut(s) 443, 550
BtsIMutI CAGTG 1 cut(s) 1026
BtuMI TCGCGA 2 cut(s) 104, 272
Cac8I GCNNGC 4 cut(s) 909, 1103, 1282, 1546
CaiI CAGNNNCTG 1 cut(s) 784
CfoI GCGC 2 cut(s) 76, 1088
Cfr10I RCCGGY 1 cut(s) 426
Cfr13I GGNCC 2 cut(s) 956, 1334
Csp6I GTAC 4 cut(s) 223, 247, 280, 400
CspAI ACCGGT 1 cut(s) 426
CviAII CATG 7 cut(s) 77, 338, 397, 418, 491, 1050, 1102
CviQI GTAC 4 cut(s) 223, 247, 280, 400
DpnI GATC 5 cut(s) 107, 194, 336, 1243, 1383
DpnII GATC 5 cut(s) 105, 192, 334, 1241, 1381
DriI GACNNNNNGTC 1 cut(s) 1050
Eam1104I CTCTTC 4 cut(s) 300, 1076, 1430, 1534
Eam1105I GACNNNNNGTC 1 cut(s) 1050
EarI CTCTTC 4 cut(s) 300, 1076, 1430, 1534
EciI GGCGGA 3 cut(s) 44, 927, 1254
Eco130I CCWWGG 1 cut(s) 1337
Eco147I AGGCCT 1 cut(s) 362
Eco24I GRGCYC 1 cut(s) 115
Eco31I GGTCTC 3 cut(s) 113, 358, 625
Eco47III AGCGCT 1 cut(s) 1087
Eco81I CCTNAGG 1 cut(s) 1161
Eco88I CYCGRG 2 cut(s) 114, 134
EcoRII CCWGG 2 cut(s) 128, 362
EcoT14I CCWWGG 1 cut(s) 1337
EcoT22I ATGCAT 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 1337
FaeI CATG 7 cut(s) 80, 341, 400, 421, 494, 1053, 1105
FaiI YATR 8 cut(s) 78, 339, 398, 419, 492, 1051, 1103, 1350
FalI AAGNNNNNCTT 2 cut(s) 1353, 1385
FatI CATG 7 cut(s) 76, 337, 396, 417, 490, 1049, 1101
FauI CCCGC 1 cut(s) 1159
FblI GTMKAC 2 cut(s) 452, 1004
FokI GGATG 2 cut(s) 430, 537
FriOI GRGCYC 1 cut(s) 115
FspBI CTAG 2 cut(s) 837, 1083
GlaI GCGC 2 cut(s) 75, 1087
GsaI CCCAGC 1 cut(s) 772
GsuI CTGGAG 1 cut(s) 1238
HaeII RGCGCY 1 cut(s) 1089
HaeIII GGCC 7 cut(s) 140, 362, 395, 877, 957, 1012, 1336
HapII CCGG 5 cut(s) 313, 346, 427, 447, 1481
HhaI GCGC 2 cut(s) 76, 1088
Hin1II CATG 7 cut(s) 80, 341, 400, 421, 494, 1053, 1105
Hin6I GCGC 2 cut(s) 74, 1086
HinP1I GCGC 2 cut(s) 74, 1086
HincII GTYRAC 4 cut(s) 453, 930, 969, 1005
HindII GTYRAC 4 cut(s) 453, 930, 969, 1005
HindIII AAGCTT 1 cut(s) 1461
HpaII CCGG 5 cut(s) 313, 346, 427, 447, 1481
HphI GGTGA 2 cut(s) 710, 1495
Hpy166II GTNNAC 7 cut(s) 249, 453, 930, 969, 1005, 1180, 1257
Hpy188III TCNNGA 5 cut(s) 103, 271, 466, 758, 1042
Hpy8I GTNNAC 7 cut(s) 249, 453, 930, 969, 1005, 1180, 1257
Hpy99I CGWCG 2 cut(s) 272, 862
HpyAV CCTTC 6 cut(s) 109, 352, 385, 987, 1005, 1501
HpyCH4III ACNGT 5 cut(s) 69, 561, 858, 1021, 1061
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 9 cut(s) 85, 481, 487, 628, 787, 911, 1049, 1101, 1139
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 7 cut(s) 80, 341, 400, 421, 494, 1053, 1105
HspAI GCGC 2 cut(s) 74, 1086
Kzo9I GATC 5 cut(s) 105, 192, 334, 1241, 1381
LguI GCTCTTC 2 cut(s) 300, 1534
LmnI GCTCC 4 cut(s) 37, 994, 1035, 1483
LweI GCATC 1 cut(s) 496
MaeI CTAG 2 cut(s) 837, 1083
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 4 cut(s) 561, 898, 1061, 1212
MalI GATC 5 cut(s) 107, 194, 336, 1243, 1383
MbiI CCGCTC 1 cut(s) 40
MboI GATC 5 cut(s) 105, 192, 334, 1241, 1381
MboII GAAGA 8 cut(s) 287, 864, 1063, 1309, 1312, 1444, 1447, 1551
MflI RGATCY 1 cut(s) 1381
MhlI GDGCHC 2 cut(s) 53, 115
MluCI AATT 6 cut(s) 10, 473, 575, 649, 1117, 1327
MmeI TCCRAC 1 cut(s) 259
Mph1103I ATGCAT 1 cut(s) 489
MroXI GAANNNNTTC 1 cut(s) 320
MseI TTAA 1 cut(s) 1502
MslI CAYNNNNRTG 2 cut(s) 56, 896
MspA1I CMGCKG 5 cut(s) 182, 460, 772, 784, 1247
MspI CCGG 5 cut(s) 313, 346, 427, 447, 1481
MspR9I CCNGG 3 cut(s) 130, 364, 448
Mva1269I GAATGC 2 cut(s) 1130, 1525
MvaI CCWGG 2 cut(s) 130, 364
MvnI CGCG 2 cut(s) 104, 272
NciI CCSGG 1 cut(s) 448
NdeII GATC 5 cut(s) 105, 192, 334, 1241, 1381
NlaIII CATG 7 cut(s) 80, 341, 400, 421, 494, 1053, 1105
NlaIV GGNNCC 6 cut(s) 91, 1112, 1155, 1335, 1383, 1479
NmeAIII GCCGAG 1 cut(s) 988
NmuCI GTSAC 2 cut(s) 561, 898
NruI TCGCGA 2 cut(s) 104, 272
NsiI ATGCAT 1 cut(s) 489
NspI RCATGY 3 cut(s) 421, 1053, 1105
OliI CACNNNNGTG 1 cut(s) 56
PaeI GCATGC 1 cut(s) 1105
PaeR7I CTCGAG 1 cut(s) 134
PceI AGGCCT 1 cut(s) 362
PciSI GCTCTTC 2 cut(s) 300, 1534
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 162
PctI GAATGC 2 cut(s) 1130, 1525
PdmI GAANNNNTTC 1 cut(s) 320
PfeI GAWTC 5 cut(s) 185, 316, 880, 886, 947
PinAI ACCGGT 1 cut(s) 426
Psp6I CCWGG 2 cut(s) 128, 362
PspFI CCCAGC 1 cut(s) 768
PspGI CCWGG 2 cut(s) 128, 362
PspN4I GGNNCC 6 cut(s) 91, 1112, 1155, 1335, 1383, 1479
PspPI GGNCC 2 cut(s) 956, 1334
PspXI VCTCGAGB 1 cut(s) 134
PstI CTGCAG 1 cut(s) 789
PstNI CAGNNNCTG 1 cut(s) 784
PsuI RGATCY 1 cut(s) 1381
PvuII CAGCTG 3 cut(s) 460, 772, 784
RruI TCGCGA 2 cut(s) 104, 272
RsaI GTAC 4 cut(s) 224, 248, 281, 401
RsaNI GTAC 4 cut(s) 223, 247, 280, 400
RseI CAYNNNNRTG 2 cut(s) 56, 896
SalI GTCGAC 2 cut(s) 451, 1003
SapI GCTCTTC 2 cut(s) 300, 1534
SaqAI TTAA 1 cut(s) 1502
Sau3AI GATC 5 cut(s) 105, 192, 334, 1241, 1381
Sau96I GGNCC 2 cut(s) 956, 1334
ScrFI CCNGG 3 cut(s) 130, 364, 448
SduI GDGCHC 2 cut(s) 53, 115
SfaNI GCATC 1 cut(s) 496
SfcI CTRYAG 1 cut(s) 785
Sfr274I CTCGAG 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 134
SmiMI CAYNNNNRTG 2 cut(s) 56, 896
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
SphI GCATGC 1 cut(s) 1105
Sse9I AATT 6 cut(s) 10, 473, 575, 649, 1117, 1327
SseBI AGGCCT 1 cut(s) 362
SspMI CTAG 2 cut(s) 837, 1083
StuI AGGCCT 1 cut(s) 362
StyD4I CCNGG 3 cut(s) 128, 362, 446
StyI CCWWGG 1 cut(s) 1337
TaaI ACNGT 5 cut(s) 69, 561, 858, 1021, 1061
TaiI ACGT 1 cut(s) 372
TaqI TCGA 9 cut(s) 135, 261, 432, 452, 465, 860, 1004, 1074, 1268
TaqII GACCGA 2 cut(s) 137, 917
TasI AATT 6 cut(s) 10, 473, 575, 649, 1117, 1327
TatI WGTACW 1 cut(s) 246
TauI GCSGC 7 cut(s) 182, 508, 511, 604, 643, 1154, 1551
TfiI GAWTC 5 cut(s) 185, 316, 880, 886, 947
Tru1I TTAA 1 cut(s) 1502
Tru9I TTAA 1 cut(s) 1502
TscAI CASTG 1 cut(s) 1026
TseFI GTSAC 2 cut(s) 561, 898
Tsp45I GTSAC 2 cut(s) 561, 898
TspDTI ATGAA 1 cut(s) 539
TspGWI ACGGA 2 cut(s) 864, 906
TspRI CASTG 1 cut(s) 1026
XapI RAATTY 1 cut(s) 649
XceI RCATGY 3 cut(s) 421, 1053, 1105
XcmI CCANNNNNNNNNTGG 1 cut(s) 642
XhoI CTCGAG 1 cut(s) 134
XmiI GTMKAC 2 cut(s) 452, 1004
XmnI GAANNNNTTC 1 cut(s) 320
XspI CTAG 2 cut(s) 837, 1083
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.