pycom10g12220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013073
Physical Location & Seq
Reverse (-)
15790173 .. 15790601
429 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 429 bp
ATGCCCTCAAATCATTCACTTCCAGTTTCTCAGGAACAATTAACAGAGCAGCCCGGGCTGCGCAGGCGGCTGTCGTCTCTGTCCCTGAAGCTCCAGCCCATCTCTTCTCCTGCAACCTCATGGGCACTAAGCCGGTCGAAATCGGTTTCAGCCATGGGAGAGTACGCCGGAGGATCCATTAGAAAATGGTGGGTCCAGAGCTGGACATGGATCATGTCCAGAAAACCCATTTTTGCCCAGGATCTGGAGATGAACGAGGAAGAAACCAAAGTGCTTGGATCCGACAACAGAGGAAGCTGGAGGCATGTGTTCTACAAAGTCCGGTCGGAGCTCAAGAAACTCGTGGGCTCTGATGTCGGAAGCCTGCCACAAACTTACAGGTACAATTTTGACGATGGGAGGACCAAAACCAAGAAAGTTTACGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

143

Amino Acids

16.24

Weight (kDa)

9.99

Isoelectric Point (pI)

62.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17300 AT4G35320
fragaria_vesca FvH4_2g16910
malus_domestica MD05G1140900.v1.1 MD11G1242200.v1.1
pyrus_communis pycom05g13600 pycom10g12220
rosa_chinensis RchiOBHm_Chr6g0281051
rosa_laevigata RLG00000013001
rosa_multiflora Rmu_sc0012869.1_g000008
rosa_roxburghii Rroxscaffold_7G00187350
rosa_rugosa Rorug06G0093600 Rorug06G0138300
rosa_samantha Rh6BG253400 Rh6CG253300 Rh6DG244900
rosa_wichuraiana Rw6G021740 Rw7G017000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 62
AccB7I CCANNNNNTGG 1 cut(s) 244
AciI CCGC 1 cut(s) 67
AclWI GGATC 6 cut(s) 168, 181, 218, 249, 273, 286
AcuI CTGAAG 1 cut(s) 107
AfaI GTAC 2 cut(s) 164, 383
AfiI CCNNNNNNNGG 1 cut(s) 244
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 4 cut(s) 91, 201, 297, 331
AluI AGCT 4 cut(s) 91, 201, 297, 331
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 81
AlwI GGATC 6 cut(s) 168, 181, 218, 249, 273, 286
AlwNI CAGNNNCTG 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 53
ApeKI GCWGC 2 cut(s) 49, 58
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 2 cut(s) 193, 402
AsuC2I CCSGG 2 cut(s) 54, 55
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 2 cut(s) 193, 402
BaeGI GKGCMC 1 cut(s) 127
BamHI GGATCC 2 cut(s) 173, 278
BanII GRGCYC 2 cut(s) 333, 350
BauI CACGAG 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 2 cut(s) 45, 61
BccI CCATC 2 cut(s) 107, 389
BciT130I CCWGG 1 cut(s) 239
BcnI CCSGG 2 cut(s) 54, 55
BcoDI GTCTC 1 cut(s) 81
BisI GCNGC 3 cut(s) 50, 59, 68
BlsI GCNGC 3 cut(s) 51, 60, 69
Bme1390I CCNGG 3 cut(s) 54, 55, 239
Bme18I GGWCC 2 cut(s) 193, 402
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 2 cut(s) 193, 402
BmiI GGNNCC 3 cut(s) 175, 194, 280
BmrFI CCNGG 3 cut(s) 54, 55, 239
BpmI CTGGAG 3 cut(s) 77, 266, 319
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 2 cut(s) 54, 55
BsaJI CCNNGG 3 cut(s) 53, 153, 237
BsaWI WCCGGW 1 cut(s) 321
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse118I RCCGGY 1 cut(s) 132
Bse1I ACTGG 1 cut(s) 23
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 3 cut(s) 53, 153, 237
BseLI CCNNNNNNNGG 1 cut(s) 244
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 1 cut(s) 23
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 2 cut(s) 45, 61
Bsh1285I CGRYCG 2 cut(s) 137, 326
BsiEI CGRYCG 2 cut(s) 137, 326
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 4 cut(s) 54, 133, 168, 322
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 244
BsmAI GTCTC 1 cut(s) 81
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 53
Bsp1286I GDGCHC 3 cut(s) 127, 333, 350
Bsp143I GATC 4 cut(s) 173, 210, 241, 278
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 1 cut(s) 67
BspCNI CTCAG 1 cut(s) 43
BspLI GGNNCC 3 cut(s) 175, 194, 280
BspPI GGATC 6 cut(s) 168, 181, 218, 249, 273, 286
BsrFI RCCGGY 1 cut(s) 132
BsrI ACTGG 1 cut(s) 23
BssAI RCCGGY 1 cut(s) 132
BssECI CCNNGG 3 cut(s) 53, 153, 237
BssMI GATC 4 cut(s) 173, 210, 241, 278
BssSI CACGAG 1 cut(s) 341
BssT1I CCWWGG 1 cut(s) 153
Bst2BI CACGAG 1 cut(s) 341
Bst2UI CCWGG 1 cut(s) 239
Bst6I CTCTTC 1 cut(s) 109
BstC8I GCNNGC 2 cut(s) 65, 365
BstDEI CTNAG 2 cut(s) 30, 128
BstDSI CCRYGG 1 cut(s) 153
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 4 cut(s) 176, 213, 244, 281
BstMAI GTCTC 1 cut(s) 81
BstMBI GATC 4 cut(s) 173, 210, 241, 278
BstMCI CGRYCG 2 cut(s) 137, 326
BstMWI GCNNNNNNNGC 4 cut(s) 55, 58, 64, 67
BstNI CCWGG 1 cut(s) 239
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 3 cut(s) 52, 53, 237
BstSLI GKGCMC 1 cut(s) 127
BstV1I GCAGC 2 cut(s) 45, 61
BstX2I RGATCY 3 cut(s) 173, 241, 278
BstYI RGATCY 3 cut(s) 173, 241, 278
BtgI CCRYGG 1 cut(s) 153
Cac8I GCNNGC 2 cut(s) 65, 365
CaiI CAGNNNCTG 1 cut(s) 244
CfoI GCGC 1 cut(s) 63
Cfr10I RCCGGY 1 cut(s) 132
Cfr13I GGNCC 2 cut(s) 193, 402
Cfr9I CCCGGG 1 cut(s) 53
Csp6I GTAC 2 cut(s) 163, 382
CviAII CATG 5 cut(s) 120, 154, 207, 214, 305
CviQI GTAC 2 cut(s) 163, 382
DdeI CTNAG 2 cut(s) 30, 128
DpnI GATC 4 cut(s) 175, 212, 243, 280
DpnII GATC 4 cut(s) 173, 210, 241, 278
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 153
Eco24I GRGCYC 2 cut(s) 333, 350
Eco47I GGWCC 2 cut(s) 193, 402
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 107
Eco88I CYCGRG 1 cut(s) 53
EcoICRI GAGCTC 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 153
EcoT38I GRGCYC 2 cut(s) 333, 350
ErhI CCWWGG 1 cut(s) 153
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 5 cut(s) 123, 157, 210, 217, 308
FaiI YATR 5 cut(s) 121, 155, 208, 215, 306
FaqI GGGAC 1 cut(s) 67
FatI CATG 5 cut(s) 119, 153, 206, 213, 304
Fnu4HI GCNGC 3 cut(s) 50, 59, 68
FriOI GRGCYC 2 cut(s) 333, 350
Fsp4HI GCNGC 3 cut(s) 50, 59, 68
FspI TGCGCA 1 cut(s) 62
GlaI GCGC 1 cut(s) 62
GluI GCNGC 3 cut(s) 50, 59, 68
GsuI CTGGAG 3 cut(s) 77, 266, 319
HapII CCGG 4 cut(s) 54, 133, 168, 322
HhaI GCGC 1 cut(s) 63
Hin1II CATG 5 cut(s) 123, 157, 210, 217, 308
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HpaII CCGG 4 cut(s) 54, 133, 168, 322
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 4 cut(s) 283, 328, 352, 359
Hpy188III TCNNGA 5 cut(s) 32, 196, 219, 245, 334
Hpy8I GTNNAC 1 cut(s) 421
HpyCH4V TGCA 1 cut(s) 113
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 58, 64, 67
HpyF3I CTNAG 2 cut(s) 30, 128
Hsp92II CATG 5 cut(s) 123, 157, 210, 217, 308
HspAI GCGC 1 cut(s) 61
Kzo9I GATC 4 cut(s) 173, 210, 241, 278
LmnI GCTCC 2 cut(s) 96, 328
Lsp1109I GCAGC 2 cut(s) 45, 61
MalI GATC 4 cut(s) 175, 212, 243, 280
MboI GATC 4 cut(s) 173, 210, 241, 278
MboII GAAGA 2 cut(s) 96, 272
MflI RGATCY 3 cut(s) 173, 241, 278
MhlI GDGCHC 3 cut(s) 127, 333, 350
MluCI AATT 2 cut(s) 38, 385
MmeI TCCRAC 3 cut(s) 306, 306, 337
MnlI CCTC 7 cut(s) 16, 127, 164, 250, 284, 294, 393
MseI TTAA 1 cut(s) 41
MspI CCGG 4 cut(s) 54, 133, 168, 322
MspR9I CCNGG 3 cut(s) 54, 55, 239
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 4 cut(s) 55, 58, 64, 67
NciI CCSGG 2 cut(s) 54, 55
NcoI CCATGG 1 cut(s) 153
NdeII GATC 4 cut(s) 173, 210, 241, 278
NlaIII CATG 5 cut(s) 123, 157, 210, 217, 308
NlaIV GGNNCC 3 cut(s) 175, 194, 280
NsbI TGCGCA 1 cut(s) 62
NspI RCATGY 1 cut(s) 308
PflMI CCANNNNNTGG 1 cut(s) 244
PkrI GCNGC 3 cut(s) 51, 60, 69
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 3 cut(s) 175, 194, 280
PspPI GGNCC 2 cut(s) 193, 402
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 3 cut(s) 173, 241, 278
RsaI GTAC 2 cut(s) 164, 383
RsaNI GTAC 2 cut(s) 163, 382
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 1 cut(s) 41
SatI GCNGC 3 cut(s) 50, 59, 68
Sau3AI GATC 4 cut(s) 173, 210, 241, 278
Sau96I GGNCC 2 cut(s) 193, 402
ScrFI CCNGG 3 cut(s) 54, 55, 239
SduI GDGCHC 3 cut(s) 127, 333, 350
SetI ASST 6 cut(s) 93, 119, 203, 299, 333, 383
SinI GGWCC 2 cut(s) 193, 402
SmaI CCCGGG 1 cut(s) 55
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SrfI GCCCGGGC 1 cut(s) 55
Sse9I AATT 2 cut(s) 38, 385
SsiI CCGC 1 cut(s) 67
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 3 cut(s) 52, 53, 237
StyI CCWWGG 1 cut(s) 153
TaqI TCGA 1 cut(s) 137
TasI AATT 2 cut(s) 38, 385
TauI GCSGC 1 cut(s) 70
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TseI GCWGC 2 cut(s) 49, 58
TspDTI ATGAA 1 cut(s) 266
TspMI CCCGGG 1 cut(s) 53
Van91I CCANNNNNTGG 1 cut(s) 244
VpaK11BI GGWCC 2 cut(s) 193, 402
XceI RCATGY 1 cut(s) 308
XmaI CCCGGG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.