pycom10g13670

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006975
Physical Location & Seq
Reverse (-)
17136363 .. 17137153
791 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 534 bp
ATGGCCCTTAATAGGTCACCTCCCTCTGCATTTATACTCGGAGCCATGGCTGATCAACACGGACCCATCTACTCCACGAAGCTTGGCCAGCACGAACTGCTTGTTCTCAGCGCTTGGGAACAAGTCCAGGAATTGTTCACAAAAAATGACAGAGTTTTTGCCACCCGGCCGAATACAGCCGTTGGCAGGTACTTGGGCTATGACAACGCCGTCTTCGCCTTTGCTCCGTACGGAGACTACTGGCGTAACGTTCGTAAAATGGCCACCATGGAGCTTCTCTCGAATTTGACGACTGACCAGTATAATGAGAAGCACGGGGACGTTTGGCGTTTTGAGAAGGCGGTAAAAGAGACTTTGTATCTCTTTGGTGTTTTTGTCTGGTCAGATGCTATGCCATGTTTTGAGTGGTTGGACGGTTTGTCTGGTCACGTGAGTTCCATAAAGAAATGTTTTAAGGAACTGGACTACGTACTTGGGAAATGGCTTGAAGAACATCGACAAAGGAGATTGGAAAGCAAGAATAATAGGCCGTGA

Protein Analysis

178

Amino Acids

20.56

Weight (kDa)

7.78

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 16 - 97 1.6e-08 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 177
AciI CCGC 1 cut(s) 341
AclI AACGTT 1 cut(s) 249
AcoI YGGCCR 3 cut(s) 85, 167, 261
AcsI RAATTY 1 cut(s) 283
AcvI CACGTG 1 cut(s) 430
AfaI GTAC 3 cut(s) 191, 230, 471
AfeI AGCGCT 1 cut(s) 112
AfiI CCNNNNNNNGG 2 cut(s) 12, 186
AgsI TTSAA 1 cut(s) 488
AhdI GACNNNNNGTC 1 cut(s) 418
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 2 cut(s) 82, 274
AluI AGCT 2 cut(s) 82, 274
Alw26I GTCTC 2 cut(s) 228, 344
Aor51HI AGCGCT 1 cut(s) 112
AoxI GGCC 5 cut(s) 3, 85, 167, 261, 527
ApoI RAATTY 1 cut(s) 283
ArsI GACNNNNNNTTYG 2 cut(s) 140, 172
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 2 cut(s) 4, 62
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 9
AvaII GGWCC 1 cut(s) 62
BalI TGGCCA 2 cut(s) 87, 263
BbrPI CACGTG 1 cut(s) 430
BbsI GAAGAC 1 cut(s) 205
BccI CCATC 1 cut(s) 74
BceAI ACGGC 3 cut(s) 164, 194, 514
BciT130I CCWGG 1 cut(s) 128
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 2 cut(s) 228, 344
BfoI RGCGCY 1 cut(s) 114
BfuAI ACCTGC 1 cut(s) 177
Bme1390I CCNGG 2 cut(s) 128, 166
Bme18I GGWCC 1 cut(s) 62
BmeRI GACNNNNNGTC 1 cut(s) 418
BmgT120I GGNCC 2 cut(s) 4, 62
BmiI GGNNCC 2 cut(s) 43, 64
BmrFI CCNGG 2 cut(s) 128, 166
BmsI GCATC 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 205
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpuMI CCSGG 1 cut(s) 166
BsaAI YACGTR 2 cut(s) 430, 469
BsaJI CCNNGG 2 cut(s) 45, 267
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 186
Bse1I ACTGG 3 cut(s) 245, 298, 465
BseBI CCWGG 1 cut(s) 128
BseDI CCNNGG 2 cut(s) 45, 267
BseLI CCNNNNNNNGG 2 cut(s) 12, 186
BseMII CTCAG 1 cut(s) 121
BseNI ACTGG 3 cut(s) 245, 298, 465
BseX3I CGGCCG 1 cut(s) 167
Bsh1285I CGRYCG 1 cut(s) 170
BshFI GGCC 5 cut(s) 5, 87, 169, 263, 529
BsiEI CGRYCG 1 cut(s) 170
BsiSI CCGG 1 cut(s) 166
BsiWI CGTACG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 12, 186
BsmAI GTCTC 2 cut(s) 228, 344
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 5 cut(s) 5, 87, 169, 263, 529
Bsp143I GATC 1 cut(s) 52
Bsp19I CCATGG 2 cut(s) 45, 267
BspACI CCGC 1 cut(s) 341
BspANI GGCC 5 cut(s) 5, 87, 169, 263, 529
BspCNI CTCAG 1 cut(s) 120
BspLI GGNNCC 2 cut(s) 43, 64
BspMI ACCTGC 1 cut(s) 177
BsrI ACTGG 3 cut(s) 245, 298, 465
BssECI CCNNGG 2 cut(s) 45, 267
BssMI GATC 1 cut(s) 52
BssT1I CCWWGG 2 cut(s) 45, 267
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 97
BstBAI YACGTR 2 cut(s) 430, 469
BstC8I GCNNGC 1 cut(s) 89
BstDEI CTNAG 1 cut(s) 107
BstDSI CCRYGG 2 cut(s) 45, 267
BstEII GGTNACC 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 1 cut(s) 55
BstMAI GTCTC 2 cut(s) 228, 344
BstMBI GATC 1 cut(s) 52
BstMCI CGRYCG 1 cut(s) 170
BstMWI GCNNNNNNNGC 3 cut(s) 88, 97, 215
BstNI CCWGG 1 cut(s) 128
BstPI GGTNACC 1 cut(s) 15
BstSCI CCNGG 2 cut(s) 126, 164
BstSNI TACGTA 1 cut(s) 469
BstV2I GAAGAC 1 cut(s) 205
BstZI CGGCCG 1 cut(s) 167
BsuRI GGCC 5 cut(s) 5, 87, 169, 263, 529
BtgI CCRYGG 2 cut(s) 45, 267
BveI ACCTGC 1 cut(s) 177
Cac8I GCNNGC 1 cut(s) 89
CfoI GCGC 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 4, 62
Csp6I GTAC 3 cut(s) 190, 229, 470
CspCI CAANNNNNGTGG 2 cut(s) 64, 99
CviAII CATG 3 cut(s) 46, 268, 396
CviQI GTAC 3 cut(s) 190, 229, 470
DdeI CTNAG 1 cut(s) 107
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
DrdI GACNNNNNNGTC 1 cut(s) 209
DriI GACNNNNNGTC 1 cut(s) 418
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 3 cut(s) 85, 167, 261
EagI CGGCCG 1 cut(s) 167
Eam1105I GACNNNNNGTC 1 cut(s) 418
EclXI CGGCCG 1 cut(s) 167
Eco105I TACGTA 1 cut(s) 469
Eco130I CCWWGG 2 cut(s) 45, 267
Eco47I GGWCC 1 cut(s) 62
Eco47III AGCGCT 1 cut(s) 112
Eco52I CGGCCG 1 cut(s) 167
Eco72I CACGTG 1 cut(s) 430
Eco91I GGTNACC 1 cut(s) 15
EcoO65I GGTNACC 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 126
EcoT14I CCWWGG 2 cut(s) 45, 267
ErhI CCWWGG 2 cut(s) 45, 267
FaeI CATG 3 cut(s) 49, 271, 399
FaiI YATR 8 cut(s) 35, 47, 201, 269, 303, 392, 397, 440
FaqI GGGAC 1 cut(s) 332
FatI CATG 3 cut(s) 45, 267, 395
FbaI TGATCA 1 cut(s) 52
GlaI GCGC 1 cut(s) 112
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 5 cut(s) 5, 87, 169, 263, 529
HapII CCGG 1 cut(s) 166
HhaI GCGC 1 cut(s) 113
Hin1II CATG 3 cut(s) 49, 271, 399
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HindIII AAGCTT 1 cut(s) 80
HpaII CCGG 1 cut(s) 166
HphI GGTGA 1 cut(s) 9
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 2 cut(s) 41, 385
Hpy188III TCNNGA 1 cut(s) 280
Hpy8I GTNNAC 1 cut(s) 138
HpyAV CCTTC 1 cut(s) 331
HpyCH4III ACNGT 1 cut(s) 416
HpyCH4IV ACGT 4 cut(s) 249, 321, 429, 468
HpyCH4V TGCA 1 cut(s) 29
HpyF10VI GCNNNNNNNGC 3 cut(s) 88, 97, 215
HpyF3I CTNAG 1 cut(s) 107
HpySE526I ACGT 4 cut(s) 249, 321, 429, 468
Hsp92II CATG 3 cut(s) 49, 271, 399
HspAI GCGC 1 cut(s) 111
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 1 cut(s) 52
LmnI GCTCC 3 cut(s) 41, 229, 271
LweI GCATC 1 cut(s) 376
MaeII ACGT 4 cut(s) 249, 321, 429, 468
MaeIII GTNAC 3 cut(s) 15, 245, 425
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 2 cut(s) 205, 500
MlsI TGGCCA 2 cut(s) 87, 263
MluCI AATT 2 cut(s) 131, 283
MluNI TGGCCA 2 cut(s) 87, 263
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 2 cut(s) 30, 34
Mox20I TGGCCA 2 cut(s) 87, 263
MscI TGGCCA 2 cut(s) 87, 263
MseI TTAA 2 cut(s) 9, 453
Msp20I TGGCCA 2 cut(s) 87, 263
MspI CCGG 1 cut(s) 166
MspR9I CCNGG 2 cut(s) 128, 166
MvaI CCWGG 1 cut(s) 128
MwoI GCNNNNNNNGC 3 cut(s) 88, 97, 215
NciI CCSGG 1 cut(s) 166
NcoI CCATGG 2 cut(s) 45, 267
NdeII GATC 1 cut(s) 52
NlaIII CATG 3 cut(s) 49, 271, 399
NlaIV GGNNCC 2 cut(s) 43, 64
NmuCI GTSAC 2 cut(s) 15, 425
PcsI WCGNNNNNNNCGW 1 cut(s) 287
Pfl23II CGTACG 1 cut(s) 228
PfoI TCCNGGA 1 cut(s) 126
PmaCI CACGTG 1 cut(s) 430
PmlI CACGTG 1 cut(s) 430
Ppu21I YACGTR 2 cut(s) 430, 469
Psp1406I AACGTT 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 126
PspCI CACGTG 1 cut(s) 430
PspEI GGTNACC 1 cut(s) 15
PspGI CCWGG 1 cut(s) 126
PspLI CGTACG 1 cut(s) 228
PspN4I GGNNCC 2 cut(s) 43, 64
PspPI GGNCC 2 cut(s) 4, 62
RsaI GTAC 3 cut(s) 191, 230, 471
RsaNI GTAC 3 cut(s) 190, 229, 470
SaqAI TTAA 2 cut(s) 9, 453
Sau3AI GATC 1 cut(s) 52
Sau96I GGNCC 2 cut(s) 4, 62
ScrFI CCNGG 2 cut(s) 128, 166
SetI ASST 9 cut(s) 17, 22, 84, 191, 252, 276, 324, 432, 471
SfaNI GCATC 1 cut(s) 376
SinI GGWCC 1 cut(s) 62
SnaBI TACGTA 1 cut(s) 469
Sse9I AATT 2 cut(s) 131, 283
SsiI CCGC 1 cut(s) 341
StyD4I CCNGG 2 cut(s) 126, 164
StyI CCWWGG 2 cut(s) 45, 267
TaaI ACNGT 1 cut(s) 416
TaiI ACGT 4 cut(s) 252, 324, 432, 471
TaqI TCGA 2 cut(s) 281, 496
TasI AATT 2 cut(s) 131, 283
Tru1I TTAA 2 cut(s) 9, 453
Tru9I TTAA 2 cut(s) 9, 453
TseFI GTSAC 2 cut(s) 15, 425
Tsp45I GTSAC 2 cut(s) 15, 425
TspGWI ACGGA 3 cut(s) 75, 216, 246
VpaK11BI GGWCC 1 cut(s) 62
XapI RAATTY 1 cut(s) 283
XcmI CCANNNNNNNNNTGG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.