pycom10g14170

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013843
Physical Location & Seq
Forward (+)
17560981 .. 17562097
1117 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 411 bp
ATGGCTGGAGACAAAAAGGTCTACACTTTGGCTGAGGTTTCCACTCACAGTGACCGCAAGGACTGCTGGCTCGTCATCGATGGCAAGGTTTATGATGTAACAAAATTCTTGGACGATCATCCTGGTGGTGATGAGGTCTTATTGGCAGTCACAGGAAAGGATGCAAGTAACGATTTCGAGGATATTGGCCACAGTTCCACTGCTAGAGCAATGCTGGACGAGTTTTACGTTGGAGACATTGATTCGGCATCCATCCCCACGAATAGGAAGTACACTCCTCCCAAGCAGCCTCACTACAACCAGGACAAGACAACTGATTTCGTCATCAAACTCCTCCAGTTCCTTGTTCCCCTGCTAATTTTGGGCCTGGCCTTCGGTGTGCGCTTCTACACCAAATCATCAACAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt-b5

Protein Analysis

137

Amino Acids

15.12

Weight (kDa)

5.22

Isoelectric Point (pI)

26.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 9 - 80 2.1e-28 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48810
fragaria_vesca FvH4_2g19040
malus_domestica MD05G1175200.v1.1 MD10G1164300.v1.1
prunus_persica Prupe.8G202900_v2.0.a1 Prupe.8G202900_v2.0.a1
pyrus_communis pycom05g16210 pycom10g14170
rosa_chinensis RchiOBHm_Chr6g0284851
rosa_laevigata RLG00000012707
rosa_multiflora Rmu_sc0000686.1_g000017
rosa_roxburghii Rroxscaffold_7G00184300
rosa_rugosa Rorug06G0167500
rosa_samantha Rh6AG280200 Rh6BG280500 Rh6CG282500
rosa_wichuraiana Rw6G024080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 21
AciI CCGC 1 cut(s) 55
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 1 cut(s) 104
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 1 cut(s) 264
AjnI CCWGG 3 cut(s) 121, 300, 366
Alw26I GTCTC 2 cut(s) 3, 228
AoxI GGCC 3 cut(s) 187, 364, 369
ApeKI GCWGC 1 cut(s) 286
ApoI RAATTY 1 cut(s) 104
AspLEI GCGC 1 cut(s) 384
AspS9I GGNCC 1 cut(s) 364
AsuHPI GGTGA 1 cut(s) 140
BalI TGGCCA 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 298
BccI CCATC 2 cut(s) 74, 260
BciT130I CCWGG 3 cut(s) 123, 302, 368
BcoDI GTCTC 2 cut(s) 3, 228
BfaI CTAG 1 cut(s) 204
BisI GCNGC 1 cut(s) 287
BlsI GCNGC 1 cut(s) 288
Bme1390I CCNGG 3 cut(s) 123, 302, 368
BmgT120I GGNCC 1 cut(s) 364
BmrFI CCNGG 3 cut(s) 123, 302, 368
BmsI GCATC 2 cut(s) 151, 257
BpmI CTGGAG 2 cut(s) 27, 320
Bpu10I CCTNAGC 1 cut(s) 33
Bsa29I ATCGAT 1 cut(s) 78
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse1I ACTGG 1 cut(s) 337
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 3 cut(s) 123, 302, 368
BseCI ATCGAT 1 cut(s) 78
BseGI GGATG 4 cut(s) 118, 166, 248, 252
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 2 cut(s) 267, 323
BseXI GCAGC 1 cut(s) 298
BshFI GGCC 3 cut(s) 189, 366, 371
BshVI ATCGAT 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 2 cut(s) 3, 228
BsnI GGCC 3 cut(s) 189, 366, 371
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 1 cut(s) 55
BspANI GGCC 3 cut(s) 189, 366, 371
BspCNI CTCAG 1 cut(s) 25
BspDI ATCGAT 1 cut(s) 78
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 1 cut(s) 337
BssMI GATC 1 cut(s) 115
Bst2UI CCWGG 3 cut(s) 123, 302, 368
Bst4CI ACNGT 2 cut(s) 50, 194
BstAPI GCANNNNNTGC 1 cut(s) 63
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 1 cut(s) 33
BstF5I GGATG 4 cut(s) 118, 166, 248, 252
BstHHI GCGC 1 cut(s) 384
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 2 cut(s) 3, 228
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstNI CCWGG 3 cut(s) 123, 302, 368
BstSCI CCNGG 3 cut(s) 121, 300, 366
BstV1I GCAGC 1 cut(s) 298
Bsu15I ATCGAT 1 cut(s) 78
BsuRI GGCC 3 cut(s) 189, 366, 371
BsuTUI ATCGAT 1 cut(s) 78
BtsCI GGATG 4 cut(s) 118, 166, 248, 252
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 55, 198
Cac8I GCNNGC 1 cut(s) 68
CfoI GCGC 1 cut(s) 384
Cfr13I GGNCC 1 cut(s) 364
ClaI ATCGAT 1 cut(s) 78
Csp6I GTAC 1 cut(s) 271
CviJI RGCY 7 cut(s) 5, 32, 70, 189, 289, 366, 371
CviKI_1 RGCY 7 cut(s) 5, 32, 70, 189, 289, 366, 371
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 1 cut(s) 33
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
DrdI GACNNNNNNGTC 1 cut(s) 17
DseDI GACNNNNNNGTC 1 cut(s) 17
EaeI YGGCCR 1 cut(s) 187
EcoRII CCWGG 3 cut(s) 121, 300, 366
FaiI YATR 2 cut(s) 93, 409
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 1 cut(s) 287
FokI GGATG 4 cut(s) 105, 173, 235, 239
Fsp4HI GCNGC 1 cut(s) 287
FspBI CTAG 1 cut(s) 204
GlaI GCGC 1 cut(s) 383
GluI GCNGC 1 cut(s) 287
GsuI CTGGAG 2 cut(s) 27, 320
HaeIII GGCC 3 cut(s) 189, 366, 371
HhaI GCGC 1 cut(s) 384
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HinfI GANTC 1 cut(s) 242
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 2 cut(s) 22, 273
Hpy8I GTNNAC 2 cut(s) 22, 273
HpyAV CCTTC 1 cut(s) 382
HpyCH4III ACNGT 2 cut(s) 50, 194
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 1 cut(s) 164
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 1 cut(s) 228
HspAI GCGC 1 cut(s) 382
Kzo9I GATC 1 cut(s) 115
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 2 cut(s) 151, 257
MaeI CTAG 1 cut(s) 204
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 4 cut(s) 50, 97, 148, 167
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MlsI TGGCCA 1 cut(s) 189
MluCI AATT 2 cut(s) 104, 357
MluNI TGGCCA 1 cut(s) 189
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 6 cut(s) 28, 127, 172, 288, 300, 344
Mox20I TGGCCA 1 cut(s) 189
MscI TGGCCA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 123
Msp20I TGGCCA 1 cut(s) 189
MspR9I CCNGG 3 cut(s) 123, 302, 368
MvaI CCWGG 3 cut(s) 123, 302, 368
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeII GATC 1 cut(s) 115
NmuCI GTSAC 2 cut(s) 50, 148
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PfeI GAWTC 1 cut(s) 242
PkrI GCNGC 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 121, 300, 366
PspGI CCWGG 3 cut(s) 121, 300, 366
PspPI GGNCC 1 cut(s) 364
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 1 cut(s) 123
SatI GCNGC 1 cut(s) 287
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 1 cut(s) 364
ScrFI CCNGG 3 cut(s) 123, 302, 368
SetI ASST 5 cut(s) 21, 39, 90, 138, 231
SfaNI GCATC 2 cut(s) 151, 257
SmiMI CAYNNNNRTG 1 cut(s) 123
Sse9I AATT 2 cut(s) 104, 357
SsiI CCGC 1 cut(s) 55
SspMI CTAG 1 cut(s) 204
StyD4I CCNGG 3 cut(s) 121, 300, 366
TaaI ACNGT 2 cut(s) 50, 194
TaiI ACGT 1 cut(s) 231
TaqI TCGA 2 cut(s) 78, 177
TasI AATT 2 cut(s) 104, 357
TatI WGTACW 1 cut(s) 270
TfiI GAWTC 1 cut(s) 242
TscAI CASTG 2 cut(s) 55, 205
TseFI GTSAC 2 cut(s) 50, 148
TseI GCWGC 1 cut(s) 286
Tsp45I GTSAC 2 cut(s) 50, 148
TspRI CASTG 2 cut(s) 55, 205
XapI RAATTY 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 21
XspI CTAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.