pycom10g21370
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003819
Physical Location & Seq
Reverse (-)
24014399 .. 24015123
725 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g21370.1

Sequence Viewer

Length: 558 bp
ATGGCCACTGTGAAGAGCTTGTACATGCTCAAGCACTGCGTGCTTGAAGAGGGGTGCGATACTTTCTACAAGGCCCACGGAGAGACTACGTTCGAGCATATGTCGAAGAGACCTGAGCTTGGTCGATTGTTCAGTGAGGCTATGACCGCTTACTCTGCTATATGTTTTGATCAAAAGGTATTCACTGACTACAGAGGTTTTGAAGAGGTGACAGAGCTGATGGACGTAGGAGGTGGCGTGGGTAATTCTCTTGCGAAAATTATCTATTCTAAAACTAGGGAGCAATTGACTGTTCATTGGGCTACAGTCAATTGTTTGCCCCTCGATTTCACGCTATTTACAACAAAAACCATCAGATCTGGGCTGGGTTGGGAAGCACTGTTTCTCTCTCATTTCTCTCAAATTCGAGACTTCTCTCTCCATTCTCTCAACCTAGCCCCAGCGCTGCTCACCCCATTCCTCTTCATAATCTCAGTTAAATCCATCGTCATGGGACCTTCTTCCTCAGACCTTCATGTGTACGTTACCTTTTCATCTAAATTAGGATCAGGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

186

Amino Acids

20.7

Weight (kDa)

6.37

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 9 - 88 5.9e-11 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 147
AclWI GGATC 1 cut(s) 553
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 402
AdeI CACNNNGTG 1 cut(s) 40
AfaI GTAC 2 cut(s) 23, 521
AfeI AGCGCT 1 cut(s) 444
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 2 cut(s) 47, 203
AluBI AGCT 3 cut(s) 18, 118, 217
AluI AGCT 3 cut(s) 18, 118, 217
Alw26I GTCTC 3 cut(s) 77, 103, 402
AlwI GGATC 1 cut(s) 553
Aor51HI AGCGCT 1 cut(s) 444
AoxI GGCC 2 cut(s) 3, 72
ApeKI GCWGC 1 cut(s) 445
ApoI RAATTY 1 cut(s) 402
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 2 cut(s) 73, 494
AsuHPI GGTGA 2 cut(s) 220, 442
AvaII GGWCC 1 cut(s) 494
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 1 cut(s) 37
BbvI GCAGC 1 cut(s) 432
BccI CCATC 3 cut(s) 214, 359, 491
BclI TGATCA 1 cut(s) 169
BcoDI GTCTC 3 cut(s) 77, 103, 402
BfaI CTAG 2 cut(s) 276, 434
BfmI CTRYAG 2 cut(s) 190, 303
BfoI RGCGCY 1 cut(s) 446
BglII AGATCT 1 cut(s) 356
BisI GCNGC 1 cut(s) 446
BlsI GCNGC 1 cut(s) 447
Bme18I GGWCC 1 cut(s) 494
BmgT120I GGNCC 2 cut(s) 73, 494
BmiI GGNNCC 1 cut(s) 495
Bpu10I CCTNAGC 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 14
BsaI GGTCTC 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 119
BseDI CCNNGG 1 cut(s) 76
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMII CTCAG 3 cut(s) 105, 486, 519
BseXI GCAGC 1 cut(s) 432
BseYI CCCAGC 2 cut(s) 364, 439
BshFI GGCC 2 cut(s) 5, 74
BslFI GGGAC 1 cut(s) 507
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 3 cut(s) 77, 103, 402
BsmFI GGGAC 1 cut(s) 507
BsnI GGCC 2 cut(s) 5, 74
Bso31I GGTCTC 1 cut(s) 103
Bsp1407I TGTACA 1 cut(s) 21
Bsp143I GATC 3 cut(s) 169, 356, 545
BspACI CCGC 1 cut(s) 147
BspANI GGCC 2 cut(s) 5, 74
BspCNI CTCAG 3 cut(s) 106, 485, 518
BspLI GGNNCC 1 cut(s) 495
BspPI GGATC 1 cut(s) 553
BspQI GCTCTTC 1 cut(s) 8
BspTNI GGTCTC 1 cut(s) 103
BsrGI TGTACA 1 cut(s) 21
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 3 cut(s) 169, 356, 545
Bst4CI ACNGT 4 cut(s) 10, 292, 307, 381
Bst6I CTCTTC 5 cut(s) 8, 42, 101, 198, 467
BstAPI GCANNNNNTGC 1 cut(s) 40
BstAUI TGTACA 1 cut(s) 21
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 3 cut(s) 114, 472, 505
BstDSI CCRYGG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 3 cut(s) 172, 359, 548
BstMAI GTCTC 3 cut(s) 77, 103, 402
BstMBI GATC 3 cut(s) 169, 356, 545
BstMWI GCNNNNNNNGC 3 cut(s) 40, 146, 155
BstNSI RCATGY 1 cut(s) 28
BstSFI CTRYAG 2 cut(s) 190, 303
BstV1I GCAGC 1 cut(s) 432
BstX2I RGATCY 1 cut(s) 356
BstXI CCANNNNNNTGG 1 cut(s) 490
BstYI RGATCY 1 cut(s) 356
BsuRI GGCC 2 cut(s) 5, 74
BtgI CCRYGG 1 cut(s) 76
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 5 cut(s) 6, 34, 139, 183, 377
Cac8I GCNNGC 1 cut(s) 41
CfoI GCGC 1 cut(s) 445
Cfr13I GGNCC 2 cut(s) 73, 494
Csp6I GTAC 2 cut(s) 22, 520
CviAII CATG 3 cut(s) 25, 490, 515
CviJI RGCY 9 cut(s) 5, 18, 74, 118, 140, 217, 302, 364, 437
CviKI_1 RGCY 9 cut(s) 5, 18, 74, 118, 140, 217, 302, 364, 437
CviQI GTAC 2 cut(s) 22, 520
DdeI CTNAG 3 cut(s) 114, 472, 505
DpnI GATC 3 cut(s) 171, 358, 547
DpnII GATC 3 cut(s) 169, 356, 545
DraIII CACNNNGTG 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 5 cut(s) 8, 42, 101, 198, 467
EarI CTCTTC 5 cut(s) 8, 42, 101, 198, 467
Eco31I GGTCTC 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 494
Eco47III AGCGCT 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 494
FaeI CATG 3 cut(s) 28, 493, 518
FaiI YATR 9 cut(s) 26, 99, 101, 143, 161, 163, 467, 491, 516
FaqI GGGAC 1 cut(s) 507
FatI CATG 3 cut(s) 24, 489, 514
FauNDI CATATG 1 cut(s) 99
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 1 cut(s) 446
Fsp4HI GCNGC 1 cut(s) 446
FspBI CTAG 2 cut(s) 276, 434
GlaI GCGC 1 cut(s) 444
GluI GCNGC 1 cut(s) 446
GsaI CCCAGC 2 cut(s) 368, 443
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 2 cut(s) 5, 74
HhaI GCGC 1 cut(s) 445
Hin1II CATG 3 cut(s) 28, 493, 518
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HphI GGTGA 2 cut(s) 220, 442
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 3 cut(s) 356, 508, 557
Hpy188III TCNNGA 1 cut(s) 407
Hpy8I GTNNAC 1 cut(s) 520
HpyAV CCTTC 2 cut(s) 507, 521
HpyCH4III ACNGT 4 cut(s) 10, 292, 307, 381
HpyCH4IV ACGT 3 cut(s) 89, 225, 522
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 146, 155
HpyF3I CTNAG 3 cut(s) 114, 472, 505
HpySE526I ACGT 3 cut(s) 89, 225, 522
Hsp92II CATG 3 cut(s) 28, 493, 518
HspAI GCGC 1 cut(s) 443
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 3 cut(s) 169, 356, 545
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 5 cut(s) 126, 345, 350, 453, 534
Lsp1109I GCAGC 1 cut(s) 432
MaeI CTAG 2 cut(s) 276, 434
MaeII ACGT 3 cut(s) 89, 225, 522
MaeIII GTNAC 2 cut(s) 208, 523
MalI GATC 3 cut(s) 171, 358, 547
MboI GATC 3 cut(s) 169, 356, 545
MboII GAAGA 6 cut(s) 25, 59, 118, 215, 454, 492
MfeI CAATTG 2 cut(s) 284, 310
MflI RGATCY 1 cut(s) 356
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 244, 258, 284, 310, 402, 539
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 8 cut(s) 43, 130, 188, 199, 224, 332, 470, 514
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 477
MslI CAYNNNNRTG 1 cut(s) 488
Msp20I TGGCCA 1 cut(s) 5
MunI CAATTG 2 cut(s) 284, 310
MwoI GCNNNNNNNGC 3 cut(s) 40, 146, 155
NdeI CATATG 1 cut(s) 99
NdeII GATC 3 cut(s) 169, 356, 545
NlaIII CATG 3 cut(s) 28, 493, 518
NlaIV GGNNCC 1 cut(s) 495
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 1 cut(s) 28
PciSI GCTCTTC 1 cut(s) 8
PkrI GCNGC 1 cut(s) 447
PpuMI RGGWCCY 1 cut(s) 494
Psp5II RGGWCCY 1 cut(s) 494
PspFI CCCAGC 2 cut(s) 364, 439
PspN4I GGNNCC 1 cut(s) 495
PspPI GGNCC 2 cut(s) 73, 494
PspPPI RGGWCCY 1 cut(s) 494
PsuI RGATCY 1 cut(s) 356
RsaI GTAC 2 cut(s) 23, 521
RsaNI GTAC 2 cut(s) 22, 520
RseI CAYNNNNRTG 1 cut(s) 488
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 1 cut(s) 446
Sau3AI GATC 3 cut(s) 169, 356, 545
Sau96I GGNCC 2 cut(s) 73, 494
SfcI CTRYAG 2 cut(s) 190, 303
SinI GGWCC 1 cut(s) 494
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 6 cut(s) 244, 258, 284, 310, 402, 539
SsiI CCGC 1 cut(s) 147
SspMI CTAG 2 cut(s) 276, 434
TaaI ACNGT 4 cut(s) 10, 292, 307, 381
TaiI ACGT 3 cut(s) 92, 228, 525
TaqI TCGA 5 cut(s) 93, 104, 124, 324, 406
TasI AATT 6 cut(s) 244, 258, 284, 310, 402, 539
TatI WGTACW 1 cut(s) 21
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 5 cut(s) 13, 41, 139, 190, 384
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 1 cut(s) 445
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 4 cut(s) 284, 454, 503, 522
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 5 cut(s) 13, 41, 139, 190, 384
VpaK11BI GGWCC 1 cut(s) 494
XapI RAATTY 1 cut(s) 402
XceI RCATGY 1 cut(s) 28
XspI CTAG 2 cut(s) 276, 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.