pycom10g21980

Eukaryotic cytochrome b561

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0011768
Physical Location & Seq
Forward (+)
24516890 .. 24518640
1751 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGCAGACTTCTCTCAAGCTAGCATCTTTCACTATTTCTACATATTATCTTCTTCTTCTTCTTCTTCTACCATTTGTTAGTTGCTCATTGCATGCAGATGTCAACCAACACAGCAGTCACGAGAACATAAAAGAAAATATTCACAAGGTGCTGTCGGTGCTTCTTGCCACCGCTGGTGCTGTGATGTCACTAAGAAACTTTGAGAACTCCTTCAACAACAACCACCAAAGATTAGGTCTAGCACTTTATGCTGCCATATGGATACAAACCCTACTAGGATTTTTCAGGCCTCGCAGGGGAAAGAAAGAAAGAAGTGTATGGTACTTGGCACATTGGATGCTTGGGACAGTGATATCCTTGGTAGGGATAATCAACATCTACACTGGATTAGAAGCCTACCATAAGAGAACACAGAGAAGCTCAGGGCTTTGGACTGTTCTTTTCACAGCTGAGGTTTCCTTCATTGCTGCGTATTATCTCTTCCAAGACAAAAGAGATTATATACAAAAACAAGGAGTGCTTTTAGGCACAACCAGGCAGCAACAGCAAGAAGATGAGGAAATTGCTCAAAGGCAGCACCAAAATCAAAAGGAGATGTTGCCAGCTCAGCAGCCTAGTGGCAAAGTCAATGCACTCAAGAACTTGTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

218

Amino Acids

24.91

Weight (kDa)

9.54

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 41 - 131 4.3e-06 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011768)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18260
fragaria_vesca FvH4_3g05770 FvH4_3g05770
malus_domestica MD05G1285600.v1.1 MD10G1263600.v1.1
prunus_persica Prupe.4G079100_v2.0.a1
pyrus_communis pycom05g26460 pycom10g21980
rosa_chinensis RchiOBHm_Chr5g0013761
rosa_laevigata RLG00000032044
rosa_multiflora Rmu_co8478607.1_g000001 Rmu_ssc0000256.1_g000002
rosa_roxburghii Rroxscaffold_1G00062880
rosa_rugosa Rorug05G0012700
rosa_samantha Rh5AG107000 Rh5BG103100 Rh5CG114900 Rh5DG101800
rosa_wichuraiana Rw5G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 171
AdeI CACNNNGTG 1 cut(s) 148
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 2 cut(s) 296, 363
AgsI TTSAA 1 cut(s) 214
AjnI CCWGG 1 cut(s) 533
AluBI AGCT 4 cut(s) 19, 420, 449, 605
AluI AGCT 4 cut(s) 19, 420, 449, 605
AoxI GGCC 1 cut(s) 287
ApeKI GCWGC 5 cut(s) 251, 467, 538, 574, 610
ArsI GACNNNNNNTTYG 2 cut(s) 220, 252
Asp700I GAANNNNTTC 2 cut(s) 138, 209
AsuNHI GCTAGC 1 cut(s) 19
BarI GAAGNNNNNNTAC 2 cut(s) 464, 496
BauI CACGAG 1 cut(s) 119
BbvCI CCTCAGC 1 cut(s) 450
BbvI GCAGC 5 cut(s) 238, 454, 550, 586, 622
BciT130I CCWGG 1 cut(s) 535
BciVI GTATCC 1 cut(s) 255
BfaI CTAG 4 cut(s) 20, 239, 275, 615
BfuI GTATCC 1 cut(s) 255
BisI GCNGC 5 cut(s) 252, 468, 539, 575, 611
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 5 cut(s) 253, 469, 540, 576, 612
Bme1390I CCNGG 1 cut(s) 535
BmrFI CCNGG 1 cut(s) 535
BmsI GCATC 2 cut(s) 32, 327
BmtI GCTAGC 1 cut(s) 23
Bpu10I CCTNAGC 2 cut(s) 421, 450
Bpu1102I GCTNAGC 1 cut(s) 606
BpuEI CTTGAG 1 cut(s) 620
BsaJI CCNNGG 1 cut(s) 357
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 363
Bse1I ACTGG 1 cut(s) 388
Bse3DI GCAATG 2 cut(s) 86, 462
BseBI CCWGG 1 cut(s) 535
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 296, 363
BseMI GCAATG 2 cut(s) 86, 462
BseMII CTCAG 3 cut(s) 435, 441, 620
BseNI ACTGG 1 cut(s) 388
BseXI GCAGC 5 cut(s) 238, 454, 550, 586, 622
BshFI GGCC 1 cut(s) 289
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 296, 363
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 1 cut(s) 289
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 1 cut(s) 171
BspANI GGCC 1 cut(s) 289
BspCNI CTCAG 3 cut(s) 434, 442, 619
BspOI GCTAGC 1 cut(s) 23
BsrDI GCAATG 2 cut(s) 86, 462
BsrI ACTGG 1 cut(s) 388
BssECI CCNNGG 1 cut(s) 357
BssSI CACGAG 1 cut(s) 119
BssT1I CCWWGG 1 cut(s) 357
Bst2BI CACGAG 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 535
Bst4CI ACNGT 2 cut(s) 349, 436
Bst6I CTCTTC 1 cut(s) 485
BstAPI GCANNNNNTGC 1 cut(s) 248
BstC8I GCNNGC 3 cut(s) 21, 93, 603
BstDEI CTNAG 4 cut(s) 191, 421, 450, 606
BstF5I GGATG 1 cut(s) 342
BstMWI GCNNNNNNNGC 4 cut(s) 157, 248, 544, 607
BstNI CCWGG 1 cut(s) 535
BstNSI RCATGY 1 cut(s) 95
BstSCI CCNGG 1 cut(s) 533
BstV1I GCAGC 5 cut(s) 238, 454, 550, 586, 622
BsuI GTATCC 1 cut(s) 255
BsuRI GGCC 1 cut(s) 289
BtsCI GGATG 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 354, 381
Cac8I GCNNGC 3 cut(s) 21, 93, 603
Csp6I GTAC 1 cut(s) 322
CviAII CATG 1 cut(s) 92
CviJI RGCY 8 cut(s) 19, 289, 395, 420, 427, 449, 605, 613
CviKI_1 RGCY 8 cut(s) 19, 289, 395, 420, 427, 449, 605, 613
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 4 cut(s) 191, 421, 450, 606
DraIII CACNNNGTG 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 1 cut(s) 289
Eco32I GATATC 1 cut(s) 354
EcoRII CCWGG 1 cut(s) 533
EcoRV GATATC 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 1 cut(s) 95
FalI AAGNNNNNCTT 2 cut(s) 504, 536
FaqI GGGAC 1 cut(s) 358
FatI CATG 1 cut(s) 91
FauNDI CATATG 1 cut(s) 257
Fnu4HI GCNGC 5 cut(s) 252, 468, 539, 575, 611
FokI GGATG 1 cut(s) 349
Fsp4HI GCNGC 5 cut(s) 252, 468, 539, 575, 611
FspBI CTAG 4 cut(s) 20, 239, 275, 615
GluI GCNGC 5 cut(s) 252, 468, 539, 575, 611
HaeIII GGCC 1 cut(s) 289
Hin1II CATG 1 cut(s) 95
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
Hpy166II GTNNAC 1 cut(s) 103
Hpy188III TCNNGA 2 cut(s) 119, 637
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 2 cut(s) 220, 469
HpyCH4III ACNGT 2 cut(s) 349, 436
HpyCH4V TGCA 4 cut(s) 4, 91, 95, 632
HpyF10VI GCNNNNNNNGC 4 cut(s) 157, 248, 544, 607
HpyF3I CTNAG 4 cut(s) 191, 421, 450, 606
Hsp92II CATG 1 cut(s) 95
LpnPI CCDG 8 cut(s) 159, 271, 280, 369, 408, 520, 547, 615
Lsp1109I GCAGC 5 cut(s) 238, 454, 550, 586, 622
LweI GCATC 2 cut(s) 32, 327
MaeI CTAG 4 cut(s) 20, 239, 275, 615
MaeIII GTNAC 2 cut(s) 116, 186
MboII GAAGA 8 cut(s) 41, 44, 47, 50, 53, 56, 472, 563
MluCI AATT 1 cut(s) 561
MnlI CCTC 3 cut(s) 300, 445, 550
MroXI GAANNNNTTC 2 cut(s) 138, 209
MslI CAYNNNNRTG 1 cut(s) 96
MspA1I CMGCKG 2 cut(s) 173, 449
MspR9I CCNGG 1 cut(s) 535
MvaI CCWGG 1 cut(s) 535
MwoI GCNNNNNNNGC 4 cut(s) 157, 248, 544, 607
NdeI CATATG 1 cut(s) 257
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 1 cut(s) 95
NmuCI GTSAC 2 cut(s) 116, 186
NspI RCATGY 1 cut(s) 95
PaeI GCATGC 1 cut(s) 95
PceI AGGCCT 1 cut(s) 289
PdmI GAANNNNTTC 2 cut(s) 138, 209
PkrI GCNGC 5 cut(s) 253, 469, 540, 576, 612
Psp6I CCWGG 1 cut(s) 533
PspGI CCWGG 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 96
SatI GCNGC 5 cut(s) 252, 468, 539, 575, 611
ScrFI CCNGG 1 cut(s) 535
SetI ASST 7 cut(s) 21, 150, 238, 422, 451, 456, 607
SfaNI GCATC 2 cut(s) 32, 327
SmiMI CAYNNNNRTG 1 cut(s) 96
SmlI CTYRAG 2 cut(s) 14, 635
SmoI CTYRAG 2 cut(s) 14, 635
SphI GCATGC 1 cut(s) 95
Sse9I AATT 1 cut(s) 561
SseBI AGGCCT 1 cut(s) 289
SsiI CCGC 1 cut(s) 171
SspI AATATT 1 cut(s) 139
SspMI CTAG 4 cut(s) 20, 239, 275, 615
StuI AGGCCT 1 cut(s) 289
StyD4I CCNGG 1 cut(s) 533
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 2 cut(s) 349, 436
TasI AATT 1 cut(s) 561
TscAI CASTG 2 cut(s) 354, 388
TseFI GTSAC 2 cut(s) 116, 186
TseI GCWGC 5 cut(s) 251, 467, 538, 574, 610
Tsp45I GTSAC 2 cut(s) 116, 186
TspDTI ATGAA 1 cut(s) 451
TspRI CASTG 2 cut(s) 354, 388
XceI RCATGY 1 cut(s) 95
XmnI GAANNNNTTC 2 cut(s) 138, 209
XspI CTAG 4 cut(s) 20, 239, 275, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.