pycom10g22220
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012414
Physical Location & Seq
Reverse (-)
24649463 .. 24651155
1693 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 981 bp
ATGTCTGATCAGGAACCTAAAGACCCTTACTACCATGACCTATTTCAGTATGAAGATCATGATGGTGGGATGGTCAGCATCAACAACCACCCAAATTTGCAAGGGTTTGATGTGAATCCTTCATACATGAACCTAACAGAGTGTTTGAAAGGATCCATGGACTATAATTCACTTGCAACGGCTTTTGGCTTGTCACCTTCAACCTCTGAAGCACTATTTTCGTCTATTGAAGGAGATCAAAAGCCTGCTAATCATCTTGGATACTCAGGTGGTGGCGGAGGCGGTGATGTGACTCCTTTGACGCCAAACTCTTCCATGTCGTCTTCTTCAGCTGAGGTTGGTGCTGATGAGGACTGTGGTAAGAATAAGAAAGATAGGCAGCCGAAAGGGTCATCAGAAGATGGAGGAGATAGCTCTAAGAAAGTGAGCAAACTAGCAAAGAATAAGGGAGAGAAAAAGCAAAGAGAGCCACGATTTGCCTTCATGACCAAGAGTGAGGTTGATCATCTAGAAGATGGATACAGATGGAGAAAATATGGGCAGAAAGCTGTGAAAAATAGCCCATATCCAAGAAGCTACTACCGGTGTACGACACCAAAATGCGGGGTCAAGAAACGCGTAGAGAGGTCGTACGAGGACCCATCCACTGTGATTACGACATACGAAGGTCAGCACAACCACCCACTTCCAGCAACCCTTAGAGGAAATGCGGCTGCAGCATTGTTCCCACCTTCTATGCTAAACCTAGCACCCGCCACGCGTACATTCTCAACCTTGGCTGCTGGTGGCCCGAGCTCTGCCTTTTCTCAGGAATTTTTGTTTCAAATGCCCCAATACATGATCAGTAACAATGCTAATCAGGGATCGTCACGAAACCCTAATTATCATCTGCAGCAGCATCAACTGCCTCATGGAGCTGCTGATCAGTATGGACTTTTGCAGGACTTGGTTCCTTCCATGTTCCTCAAACAAGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0006979 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010228 GO:0010453 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0033554 GO:0033993 GO:0034599 GO:0034614 GO:0035690 GO:0042221 GO:0042493 GO:0042542 GO:0042659 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0045595 GO:0045893 GO:0045935 GO:0046677 GO:0047484 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0080090 GO:0080134 GO:0097159 GO:0097237 GO:0097305 GO:0098542 GO:0140110 GO:1901000 GO:1901002 GO:1901363 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WRKY

Protein Analysis

327

Amino Acids

35.78

Weight (kDa)

6.56

Isoelectric Point (pI)

59.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 171 - 228 6.5e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29860 AT4G18170 AT5G46350
fragaria_vesca FvH4_3g06200
malus_domestica MD05G1290300.v1.1 MD10G1266400.v1.1
prunus_persica Prupe.4G075400_v2.0.a1 Prupe.4G075400_v2.0.a1
pyrus_communis pycom05g26710 pycom10g22220
rosa_chinensis RchiOBHm_Chr5g0013131
rosa_laevigata RLG00000031991
rosa_multiflora Rmu_sc0003892.1_g000004
rosa_roxburghii Rroxscaffold_1G00063450
rosa_samantha Rh5AG101600 Rh5BG098000 Rh5CG110100 Rh5DG097200
rosa_wichuraiana Rw5G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 618, 760
AciI CCGC 5 cut(s) 276, 282, 603, 710, 753
AclWI GGATC 3 cut(s) 147, 160, 871
AcsI RAATTY 2 cut(s) 94, 812
AcuI CTGAAG 2 cut(s) 228, 312
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 3 cut(s) 589, 632, 763
AfiI CCNNNNNNNGG 1 cut(s) 602
AflIII ACRYGT 2 cut(s) 616, 758
AgeI ACCGGT 1 cut(s) 582
AgsI TTSAA 4 cut(s) 148, 201, 230, 824
AluBI AGCT 6 cut(s) 332, 414, 548, 576, 795, 917
AluI AGCT 6 cut(s) 332, 414, 548, 576, 795, 917
Alw21I GWGCWC 1 cut(s) 797
AlwI GGATC 3 cut(s) 147, 160, 871
Ama87I CYCGRG 1 cut(s) 790
AoxI GGCC 1 cut(s) 787
ApeKI GCWGC 7 cut(s) 379, 713, 716, 779, 892, 895, 917
ApoI RAATTY 2 cut(s) 94, 812
AsiGI ACCGGT 1 cut(s) 582
AspS9I GGNCC 2 cut(s) 637, 788
AsuHPI GGTGA 2 cut(s) 186, 296
AvaI CYCGRG 1 cut(s) 790
AvaII GGWCC 1 cut(s) 637
BaeI ACNNNNGTAYC 2 cut(s) 253, 286
BamHI GGATCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 797
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 797
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 7 cut(s) 391, 700, 728, 766, 904, 904, 907
BccI CCATC 6 cut(s) 56, 64, 395, 509, 519, 649
BceAI ACGGC 1 cut(s) 195
BcgI CGANNNNNNTGC 2 cut(s) 201, 235
BciVI GTATCC 2 cut(s) 254, 512
BclI TGATCA 4 cut(s) 7, 502, 840, 922
BfaI CTAG 3 cut(s) 434, 509, 746
BfmI CTRYAG 2 cut(s) 714, 890
BfuI GTATCC 2 cut(s) 254, 512
BisI GCNGC 8 cut(s) 380, 711, 714, 717, 780, 893, 896, 918
BlsI GCNGC 8 cut(s) 381, 712, 715, 718, 781, 894, 897, 919
Bme18I GGWCC 1 cut(s) 637
BmeT110I CYCGRG 1 cut(s) 790
BmgT120I GGNCC 2 cut(s) 637, 788
BmiI GGNNCC 4 cut(s) 15, 154, 639, 951
BmsI GCATC 2 cut(s) 87, 907
BpiI GAAGAC 1 cut(s) 315
Bpu10I CCTNAGC 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 156, 774
BsaWI WCCGGW 1 cut(s) 582
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse118I RCCGGY 1 cut(s) 582
Bse8I GATNNNNATC 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 156, 774
BseGI GGATG 2 cut(s) 75, 641
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMII CTCAG 3 cut(s) 279, 324, 821
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 7 cut(s) 391, 700, 728, 766, 904, 904, 907
Bsh1236I CGCG 2 cut(s) 618, 760
BshFI GGCC 1 cut(s) 789
BshTI ACCGGT 1 cut(s) 582
BsiHKAI GWGCWC 1 cut(s) 797
BsiHKCI CYCGRG 1 cut(s) 790
BsiSI CCGG 1 cut(s) 583
BsiWI CGTACG 1 cut(s) 630
BslI CCNNNNNNNGG 1 cut(s) 602
BsnI GGCC 1 cut(s) 789
BsoBI CYCGRG 1 cut(s) 790
Bsp1286I GDGCHC 1 cut(s) 797
Bsp143I GATC 8 cut(s) 7, 55, 152, 235, 502, 840, 863, 922
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 5 cut(s) 276, 282, 603, 710, 753
BspANI GGCC 1 cut(s) 789
BspCNI CTCAG 3 cut(s) 278, 325, 820
BspFNI CGCG 2 cut(s) 618, 760
BspHI TCATGA 2 cut(s) 58, 483
BspLI GGNNCC 4 cut(s) 15, 154, 639, 951
BspMAI CTGCAG 2 cut(s) 718, 894
BspPI GGATC 3 cut(s) 147, 160, 871
BsrFI RCCGGY 1 cut(s) 582
BssAI RCCGGY 1 cut(s) 582
BssECI CCNNGG 2 cut(s) 156, 774
BssMI GATC 8 cut(s) 7, 55, 152, 235, 502, 840, 863, 922
BssNI GRCGYC 1 cut(s) 302
BssT1I CCWWGG 2 cut(s) 156, 774
Bst4CI ACNGT 2 cut(s) 356, 649
Bst6I CTCTTC 1 cut(s) 316
BstACI GRCGYC 1 cut(s) 302
BstAPI GCANNNNNTGC 1 cut(s) 904
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 5 cut(s) 265, 333, 417, 698, 807
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 2 cut(s) 75, 641
BstFNI CGCG 2 cut(s) 618, 760
BstKTI GATC 8 cut(s) 10, 58, 155, 238, 505, 843, 866, 925
BstMBI GATC 8 cut(s) 7, 55, 152, 235, 502, 840, 863, 922
BstMWI GCNNNNNNNGC 3 cut(s) 466, 716, 904
BstSFI CTRYAG 2 cut(s) 714, 890
BstUI CGCG 2 cut(s) 618, 760
BstV1I GCAGC 7 cut(s) 391, 700, 728, 766, 904, 904, 907
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
BsuI GTATCC 2 cut(s) 254, 512
BsuRI GGCC 1 cut(s) 789
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 2 cut(s) 75, 641
BtsIMutI CAGTG 1 cut(s) 645
Cac8I GCNNGC 1 cut(s) 246
CciI TCATGA 2 cut(s) 58, 483
Cfr10I RCCGGY 1 cut(s) 582
Cfr13I GGNCC 2 cut(s) 637, 788
CseI GACGC 1 cut(s) 310
Csp6I GTAC 3 cut(s) 588, 631, 762
CspAI ACCGGT 1 cut(s) 582
CviAII CATG 9 cut(s) 35, 59, 127, 157, 316, 484, 838, 911, 958
CviQI GTAC 3 cut(s) 588, 631, 762
DdeI CTNAG 5 cut(s) 265, 333, 417, 698, 807
DpnI GATC 8 cut(s) 9, 57, 154, 237, 504, 842, 865, 924
DpnII GATC 8 cut(s) 7, 55, 152, 235, 502, 840, 863, 922
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
EciI GGCGGA 1 cut(s) 291
Ecl136II GAGCTC 1 cut(s) 795
Eco130I CCWWGG 2 cut(s) 156, 774
Eco24I GRGCYC 1 cut(s) 797
Eco47I GGWCC 1 cut(s) 637
Eco53kI GAGCTC 1 cut(s) 795
Eco57I CTGAAG 2 cut(s) 228, 312
Eco88I CYCGRG 1 cut(s) 790
EcoICRI GAGCTC 1 cut(s) 795
EcoO109I RGGNCCY 1 cut(s) 637
EcoT14I CCWWGG 2 cut(s) 156, 774
EcoT38I GRGCYC 1 cut(s) 797
ErhI CCWWGG 2 cut(s) 156, 774
FaeI CATG 9 cut(s) 38, 62, 130, 160, 319, 487, 841, 914, 961
FatI CATG 9 cut(s) 34, 58, 126, 156, 315, 483, 837, 910, 957
FauI CCCGC 2 cut(s) 596, 760
FbaI TGATCA 4 cut(s) 7, 502, 840, 922
Fnu4HI GCNGC 8 cut(s) 380, 711, 714, 717, 780, 893, 896, 918
FokI GGATG 2 cut(s) 82, 628
FriOI GRGCYC 1 cut(s) 797
Fsp4HI GCNGC 8 cut(s) 380, 711, 714, 717, 780, 893, 896, 918
FspBI CTAG 3 cut(s) 434, 509, 746
GluI GCNGC 8 cut(s) 380, 711, 714, 717, 780, 893, 896, 918
HaeIII GGCC 1 cut(s) 789
HapII CCGG 1 cut(s) 583
HgaI GACGC 1 cut(s) 310
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 9 cut(s) 38, 62, 130, 160, 319, 487, 841, 914, 961
HinfI GANTC 2 cut(s) 115, 292
HpaII CCGG 1 cut(s) 583
HphI GGTGA 2 cut(s) 186, 296
Hpy166II GTNNAC 1 cut(s) 588
Hpy188I TCNGA 3 cut(s) 7, 208, 397
Hpy188III TCNNGA 7 cut(s) 11, 59, 484, 509, 610, 809, 870
Hpy8I GTNNAC 1 cut(s) 588
HpyAV CCTTC 7 cut(s) 129, 207, 224, 490, 659, 741, 963
HpyCH4III ACNGT 2 cut(s) 356, 649
HpyCH4V TGCA 5 cut(s) 100, 176, 716, 892, 940
HpyF10VI GCNNNNNNNGC 3 cut(s) 466, 716, 904
HpyF3I CTNAG 5 cut(s) 265, 333, 417, 698, 807
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 9 cut(s) 38, 62, 130, 160, 319, 487, 841, 914, 961
Ksp22I TGATCA 4 cut(s) 7, 502, 840, 922
Kzo9I GATC 8 cut(s) 7, 55, 152, 235, 502, 840, 863, 922
LmnI GCTCC 1 cut(s) 914
LpnPI CCDG 8 cut(s) 252, 258, 596, 702, 768, 794, 845, 926
Lsp1109I GCAGC 7 cut(s) 391, 700, 728, 766, 904, 904, 907
LweI GCATC 2 cut(s) 87, 907
MaeI CTAG 3 cut(s) 434, 509, 746
MaeIII GTNAC 4 cut(s) 192, 289, 845, 867
MalI GATC 8 cut(s) 9, 57, 154, 237, 504, 842, 865, 924
MboI GATC 8 cut(s) 7, 55, 152, 235, 502, 840, 863, 922
MboII GAAGA 6 cut(s) 65, 303, 315, 318, 410, 524
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 1 cut(s) 797
MluCI AATT 4 cut(s) 94, 166, 812, 880
MluI ACGCGT 2 cut(s) 616, 758
MlyI GAGTC 1 cut(s) 286
MslI CAYNNNNRTG 2 cut(s) 63, 598
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 1 cut(s) 583
MvnI CGCG 2 cut(s) 618, 760
MwoI GCNNNNNNNGC 3 cut(s) 466, 716, 904
NcoI CCATGG 1 cut(s) 156
NdeII GATC 8 cut(s) 7, 55, 152, 235, 502, 840, 863, 922
NlaIII CATG 9 cut(s) 38, 62, 130, 160, 319, 487, 841, 914, 961
NlaIV GGNNCC 4 cut(s) 15, 154, 639, 951
NmuCI GTSAC 3 cut(s) 192, 289, 867
PagI TCATGA 2 cut(s) 58, 483
PfeI GAWTC 1 cut(s) 115
Pfl23II CGTACG 1 cut(s) 630
PinAI ACCGGT 1 cut(s) 582
PkrI GCNGC 8 cut(s) 381, 712, 715, 718, 781, 894, 897, 919
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PpuMI RGGWCCY 1 cut(s) 637
Psp124BI GAGCTC 1 cut(s) 797
Psp5II RGGWCCY 1 cut(s) 637
PspLI CGTACG 1 cut(s) 630
PspN4I GGNNCC 4 cut(s) 15, 154, 639, 951
PspPI GGNCC 2 cut(s) 637, 788
PspPPI RGGWCCY 1 cut(s) 637
PstI CTGCAG 2 cut(s) 718, 894
PsuI RGATCY 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 3 cut(s) 589, 632, 763
RsaNI GTAC 3 cut(s) 588, 631, 762
RseI CAYNNNNRTG 2 cut(s) 63, 598
SacI GAGCTC 1 cut(s) 797
SatI GCNGC 8 cut(s) 380, 711, 714, 717, 780, 893, 896, 918
Sau3AI GATC 8 cut(s) 7, 55, 152, 235, 502, 840, 863, 922
Sau96I GGNCC 2 cut(s) 637, 788
SchI GAGTC 1 cut(s) 286
SduI GDGCHC 1 cut(s) 797
SfaNI GCATC 2 cut(s) 87, 907
SfcI CTRYAG 2 cut(s) 714, 890
SinI GGWCC 1 cut(s) 637
SmiMI CAYNNNNRTG 2 cut(s) 63, 598
Sse9I AATT 4 cut(s) 94, 166, 812, 880
SsiI CCGC 5 cut(s) 276, 282, 603, 710, 753
SspMI CTAG 3 cut(s) 434, 509, 746
SstI GAGCTC 1 cut(s) 797
StyI CCWWGG 2 cut(s) 156, 774
TaaI ACNGT 2 cut(s) 356, 649
TasI AATT 4 cut(s) 94, 166, 812, 880
TauI GCSGC 1 cut(s) 713
TfiI GAWTC 1 cut(s) 115
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 3 cut(s) 192, 289, 867
TseI GCWGC 7 cut(s) 379, 713, 716, 779, 892, 895, 917
Tsp45I GTSAC 3 cut(s) 192, 289, 867
TspDTI ATGAA 4 cut(s) 66, 111, 143, 472
TspRI CASTG 1 cut(s) 652
VpaK11BI GGWCC 1 cut(s) 637
XapI RAATTY 2 cut(s) 94, 812
XbaI TCTAGA 1 cut(s) 508
XspI CTAG 3 cut(s) 434, 509, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.