pycom10g22250

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0014427
Physical Location & Seq
Forward (+)
24673437 .. 24674300
864 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 864 bp
ATGGCTATGGTGGCCACCAACATACTCTCACTCTACCACCTCTCCTCCACTCTCCAAACCCCAAAACCCCCAACTCCCCAGCCAGTCCCTGACCACCTCCTCCACCAGCTCCACACATTACGCGCCACCATCAACCACCTCACGCGCGACCACCCTTCACCATCAACCGCCACCGCCAAAACAACCAAACCCGACATCCCCTCAGATCTCCTCCTCTACTCTCAGCTCTCCCCCATCGCCTCCTCCTGCCACAACCACCCTGACCTCGTCCACAAGTACATGACCTACACCCCCTTCTCTATCTGCCCACTCGACTCCGACCTTGCCGAGGCGCTCATTCTCCGCGGCTGCCACCCGCTCCCCCGCCGTCGATGCTTCACAAGAACCCCACCAAAACCCACTTCTTCTCTCGCCCTGAATCCGTTTCCTCCTTCGCTTCCGGACTCCAATGTCTTGTGGGACAAGTATTCCTGCAAAAGCTTCAGCTGCCTCGGCGGCAAAAACCCCAACTTGGGCTTCGACCTGCCTCACGAAGTGTCCAATTTCATGACTTACAAGTCGGAGCTCGACCTCCCAATCCCTCAGCTCTTCCAAATCGCCAAAGCAGCCAACTCGGTCCTCCGTCTCGGCGTCGACATCGGCGGCGGAACTGGGACTTTCGCGGCGAGGATGAAGCTTTACAATGTCACCGTTGTCACGACCACGATGAACCTCGGAATCCCCAACAATGAGGCGGTGGCGCTGAGAGGGTTGGTCCCAATTCACGCGCCGCTGCAGCAGCGGTTGCCAGTGTTCGACGGGGTGGTGGATCTGGTGCGGTGCGGGCACGCAGTGAACCGGTGGATACCGGTGACGGCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_29

Protein Analysis

288

Amino Acids

31.44

Weight (kDa)

8.98

Isoelectric Point (pI)

53.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29790 AT1G29790
fragaria_vesca FvH4_3g06240 FvH4_3g06240
malus_domestica MD05G1290600.v1.1 MD16G1203100.v1.1
prunus_persica Prupe.4G075100_v2.0.a1
pyrus_communis pycom10g22250
rosa_chinensis RchiOBHm_Chr5g0013071
rosa_laevigata RLG00000031983
rosa_multiflora Rmu_sc0003892.1_g000015
rosa_rugosa Rorug05G0007000
rosa_samantha Rh5AG101000 Rh5BG097600 Rh5CG109700 Rh5DG096600
rosa_wichuraiana Rw5G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 449, 556
Acc36I ACCTGC 1 cut(s) 531
AccBSI CCGCTC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 633
AccII CGCG 6 cut(s) 123, 145, 147, 345, 662, 767
AccIII TCCGGA 1 cut(s) 439
AclWI GGATC 1 cut(s) 816
AcoI YGGCCR 1 cut(s) 12
AcuI CTGAAG 1 cut(s) 466
AcyI GRCGYC 1 cut(s) 630
AdeI CACNNNGTG 2 cut(s) 535, 832
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 3 cut(s) 328, 511, 512
AgeI ACCGGT 2 cut(s) 837, 847
AjuI GAANNNNNNNTTGG 4 cut(s) 385, 417, 500, 532
AluBI AGCT 7 cut(s) 109, 226, 480, 486, 565, 586, 676
AluI AGCT 7 cut(s) 109, 226, 480, 486, 565, 586, 676
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 1 cut(s) 816
AlwNI CAGNNNCTG 1 cut(s) 89
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 1 cut(s) 12
ApeKI GCWGC 6 cut(s) 348, 486, 605, 772, 775, 778
AsiGI ACCGGT 2 cut(s) 837, 847
AspLEI GCGC 5 cut(s) 125, 147, 334, 742, 769
AspS9I GGNCC 2 cut(s) 616, 754
AsuHPI GGTGA 3 cut(s) 150, 679, 862
AvaII GGWCC 2 cut(s) 616, 754
BaeGI GKGCMC 1 cut(s) 828
BalI TGGCCA 1 cut(s) 14
BanII GRGCYC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 567
BbvCI CCTCAGC 1 cut(s) 582
BbvI GCAGC 6 cut(s) 335, 473, 617, 759, 787, 790
BccI CCATC 3 cut(s) 137, 169, 242
BceAI ACGGC 1 cut(s) 351
BcgI CGANNNNNNTGC 2 cut(s) 594, 628
BciVI GTATCC 1 cut(s) 837
BcoDI GTCTC 1 cut(s) 629
BfmI CTRYAG 1 cut(s) 773
BfoI RGCGCY 2 cut(s) 335, 743
BfuAI ACCTGC 1 cut(s) 531
BfuI GTATCC 1 cut(s) 837
BglI GCCNNNNNGGC 1 cut(s) 495
BglII AGATCT 1 cut(s) 205
Bme18I GGWCC 2 cut(s) 616, 754
BmgT120I GGNCC 2 cut(s) 616, 754
BmiI GGNNCC 1 cut(s) 756
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 1 cut(s) 362
BmuI ACTGGG 1 cut(s) 660
Bpu10I CCTNAGC 1 cut(s) 582
BsaHI GRCGYC 1 cut(s) 630
BsaJI CCNNGG 4 cut(s) 327, 343, 490, 712
BsaWI WCCGGW 3 cut(s) 439, 837, 847
BsaXI ACNNNNNCTCC 4 cut(s) 26, 29, 56, 59
Bsc4I CCNNNNNNNGG 3 cut(s) 328, 511, 512
Bse118I RCCGGY 2 cut(s) 837, 847
Bse1I ACTGG 3 cut(s) 83, 655, 788
BseAI TCCGGA 1 cut(s) 439
BseDI CCNNGG 4 cut(s) 327, 343, 490, 712
BseGI GGATG 2 cut(s) 195, 675
BseLI CCNNNNNNNGG 3 cut(s) 328, 511, 512
BseMII CTCAG 4 cut(s) 216, 236, 596, 734
BseNI ACTGG 3 cut(s) 83, 655, 788
BseRI GAGGAG 5 cut(s) 34, 89, 200, 203, 232
BseSI GKGCMC 1 cut(s) 828
BseXI GCAGC 6 cut(s) 335, 473, 617, 759, 787, 790
BseYI CCCAGC 1 cut(s) 78
Bsh1236I CGCG 6 cut(s) 123, 145, 147, 345, 662, 767
BshFI GGCC 1 cut(s) 14
BshTI ACCGGT 2 cut(s) 837, 847
BsiHKAI GWGCWC 1 cut(s) 567
BsiSI CCGG 3 cut(s) 440, 838, 848
BslFI GGGAC 4 cut(s) 71, 473, 667, 740
BslI CCNNNNNNNGG 3 cut(s) 328, 511, 512
BsmAI GTCTC 1 cut(s) 629
BsmBI CGTCTC 1 cut(s) 629
BsmFI GGGAC 4 cut(s) 71, 473, 667, 740
BsnI GGCC 1 cut(s) 14
Bsp1286I GDGCHC 2 cut(s) 567, 828
Bsp13I TCCGGA 1 cut(s) 439
Bsp143I GATC 2 cut(s) 205, 808
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 4 cut(s) 215, 235, 595, 735
BspEI TCCGGA 1 cut(s) 439
BspFNI CGCG 6 cut(s) 123, 145, 147, 345, 662, 767
BspHI TCATGA 1 cut(s) 546
BspLI GGNNCC 1 cut(s) 756
BspMAI CTGCAG 1 cut(s) 777
BspMI ACCTGC 1 cut(s) 531
BspPI GGATC 1 cut(s) 816
BspQI GCTCTTC 1 cut(s) 593
BsrBI CCGCTC 1 cut(s) 358
BsrFI RCCGGY 2 cut(s) 837, 847
BsrI ACTGG 3 cut(s) 83, 655, 788
BssAI RCCGGY 2 cut(s) 837, 847
BssECI CCNNGG 4 cut(s) 327, 343, 490, 712
BssMI GATC 2 cut(s) 205, 808
BssNI GRCGYC 1 cut(s) 630
Bst4CI ACNGT 1 cut(s) 691
Bst6I CTCTTC 1 cut(s) 593
BstACI GRCGYC 1 cut(s) 630
BstAPI GCANNNNNTGC 1 cut(s) 784
BstC8I GCNNGC 2 cut(s) 824, 828
BstDEI CTNAG 4 cut(s) 202, 222, 582, 743
BstDSI CCRYGG 1 cut(s) 343
BstENI CCTNNNNNAGG 1 cut(s) 326
BstF5I GGATG 2 cut(s) 195, 675
BstFNI CGCG 6 cut(s) 123, 145, 147, 345, 662, 767
BstH2I RGCGCY 2 cut(s) 335, 743
BstHHI GCGC 5 cut(s) 125, 147, 334, 742, 769
BstKTI GATC 2 cut(s) 208, 811
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 2 cut(s) 205, 808
BstSFI CTRYAG 1 cut(s) 773
BstSLI GKGCMC 1 cut(s) 828
BstUI CGCG 6 cut(s) 123, 145, 147, 345, 662, 767
BstV1I GCAGC 6 cut(s) 335, 473, 617, 759, 787, 790
BstX2I RGATCY 2 cut(s) 205, 808
BstYI RGATCY 2 cut(s) 205, 808
BsuI GTATCC 1 cut(s) 837
BsuRI GGCC 1 cut(s) 14
BtgI CCRYGG 1 cut(s) 343
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 2 cut(s) 195, 675
BtsI GCAGTG 1 cut(s) 837
BtsIMutI CAGTG 2 cut(s) 795, 837
BveI ACCTGC 1 cut(s) 531
Cac8I GCNNGC 2 cut(s) 824, 828
CaiI CAGNNNCTG 1 cut(s) 89
CciI TCATGA 1 cut(s) 546
CfoI GCGC 5 cut(s) 125, 147, 334, 742, 769
Cfr10I RCCGGY 2 cut(s) 837, 847
Cfr13I GGNCC 2 cut(s) 616, 754
Cfr42I CCGCGG 1 cut(s) 346
CseI GACGC 1 cut(s) 619
Csp6I GTAC 1 cut(s) 277
CspAI ACCGGT 2 cut(s) 837, 847
CviAII CATG 2 cut(s) 280, 547
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 4 cut(s) 202, 222, 582, 743
DpnI GATC 2 cut(s) 207, 810
DpnII GATC 2 cut(s) 205, 808
DraIII CACNNNGTG 2 cut(s) 535, 832
DrdI GACNNNNNNGTC 2 cut(s) 449, 556
DseDI GACNNNNNNGTC 2 cut(s) 449, 556
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
EciI GGCGGA 1 cut(s) 660
Ecl136II GAGCTC 1 cut(s) 565
Eco24I GRGCYC 1 cut(s) 567
Eco47I GGWCC 2 cut(s) 616, 754
Eco53kI GAGCTC 1 cut(s) 565
Eco57I CTGAAG 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 565
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoT38I GRGCYC 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 629
FaeI CATG 2 cut(s) 283, 550
FaiI YATR 4 cut(s) 8, 23, 281, 548
FaqI GGGAC 4 cut(s) 71, 473, 667, 740
FatI CATG 2 cut(s) 279, 546
FauI CCCGC 3 cut(s) 363, 371, 815
FblI GTMKAC 1 cut(s) 633
FokI GGATG 2 cut(s) 182, 682
FriOI GRGCYC 1 cut(s) 567
GlaI GCGC 5 cut(s) 124, 146, 333, 741, 768
GsaI CCCAGC 1 cut(s) 82
HaeII RGCGCY 2 cut(s) 335, 743
HaeIII GGCC 1 cut(s) 14
HapII CCGG 3 cut(s) 440, 838, 848
HgaI GACGC 1 cut(s) 619
HhaI GCGC 5 cut(s) 125, 147, 334, 742, 769
Hin1I GRCGYC 1 cut(s) 630
Hin1II CATG 2 cut(s) 283, 550
Hin6I GCGC 5 cut(s) 123, 145, 332, 740, 767
HinP1I GCGC 5 cut(s) 123, 145, 332, 740, 767
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 2 cut(s) 478, 674
HinfI GANTC 4 cut(s) 314, 418, 443, 717
HpaII CCGG 3 cut(s) 440, 838, 848
HphI GGTGA 3 cut(s) 150, 679, 862
Hpy166II GTNNAC 3 cut(s) 271, 634, 835
Hpy188I TCNGA 4 cut(s) 205, 319, 562, 716
Hpy188III TCNNGA 4 cut(s) 440, 530, 547, 697
Hpy8I GTNNAC 3 cut(s) 271, 634, 835
Hpy99I CGWCG 3 cut(s) 372, 635, 800
HpyAV CCTTC 3 cut(s) 165, 304, 441
HpyCH4III ACNGT 1 cut(s) 691
HpyCH4V TGCA 2 cut(s) 474, 775
HpyF3I CTNAG 4 cut(s) 202, 222, 582, 743
Hsp92I GRCGYC 1 cut(s) 630
Hsp92II CATG 2 cut(s) 283, 550
HspAI GCGC 5 cut(s) 123, 145, 332, 740, 767
Kpn2I TCCGGA 1 cut(s) 439
KspI CCGCGG 1 cut(s) 346
Kzo9I GATC 2 cut(s) 205, 808
LguI GCTCTTC 1 cut(s) 593
LmnI GCTCC 3 cut(s) 114, 363, 562
Lsp1109I GCAGC 6 cut(s) 335, 473, 617, 759, 787, 790
LweI GCATC 1 cut(s) 362
MaeIII GTNAC 3 cut(s) 685, 694, 850
MalI GATC 2 cut(s) 207, 810
MbiI CCGCTC 1 cut(s) 358
MboI GATC 2 cut(s) 205, 808
MboII GAAGA 2 cut(s) 396, 580
MflI RGATCY 2 cut(s) 205, 808
MhlI GDGCHC 2 cut(s) 567, 828
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 2 cut(s) 541, 759
MluNI TGGCCA 1 cut(s) 14
MlyI GAGTC 2 cut(s) 308, 437
MmeI TCCRAC 2 cut(s) 342, 540
Mox20I TGGCCA 1 cut(s) 14
MroI TCCGGA 1 cut(s) 439
MscI TGGCCA 1 cut(s) 14
Msp20I TGGCCA 1 cut(s) 14
MspA1I CMGCKG 4 cut(s) 345, 486, 772, 781
MspI CCGG 3 cut(s) 440, 838, 848
MvnI CGCG 6 cut(s) 123, 145, 147, 345, 662, 767
NdeII GATC 2 cut(s) 205, 808
NlaIII CATG 2 cut(s) 283, 550
NlaIV GGNNCC 1 cut(s) 756
NmeAIII GCCGAG 3 cut(s) 352, 471, 606
NmuCI GTSAC 3 cut(s) 685, 694, 850
PagI TCATGA 1 cut(s) 546
PciSI GCTCTTC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 418, 717
PflFI GACNNNGTC 1 cut(s) 266
PinAI ACCGGT 2 cut(s) 837, 847
PleI GAGTC 2 cut(s) 308, 437
PpsI GAGTC 2 cut(s) 308, 437
Psp124BI GAGCTC 1 cut(s) 567
PspFI CCCAGC 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 756
PspPI GGNCC 2 cut(s) 616, 754
PstI CTGCAG 1 cut(s) 777
PstNI CAGNNNCTG 1 cut(s) 89
PsuI RGATCY 2 cut(s) 205, 808
PsyI GACNNNGTC 1 cut(s) 266
PvuII CAGCTG 1 cut(s) 486
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SacI GAGCTC 1 cut(s) 567
SacII CCGCGG 1 cut(s) 346
SalI GTCGAC 1 cut(s) 632
SapI GCTCTTC 1 cut(s) 593
Sau3AI GATC 2 cut(s) 205, 808
Sau96I GGNCC 2 cut(s) 616, 754
SchI GAGTC 2 cut(s) 308, 437
SduI GDGCHC 2 cut(s) 567, 828
SfaNI GCATC 1 cut(s) 362
SfcI CTRYAG 1 cut(s) 773
Sfr303I CCGCGG 1 cut(s) 346
SgrBI CCGCGG 1 cut(s) 346
SinI GGWCC 2 cut(s) 616, 754
Sse9I AATT 2 cut(s) 541, 759
SstI GAGCTC 1 cut(s) 567
TaaI ACNGT 1 cut(s) 691
TaqI TCGA 6 cut(s) 312, 370, 519, 567, 633, 795
TaqII GACCGA 1 cut(s) 604
TasI AATT 2 cut(s) 541, 759
TatI WGTACW 1 cut(s) 276
TauI GCSGC 5 cut(s) 348, 498, 645, 665, 772
TfiI GAWTC 2 cut(s) 418, 717
TscAI CASTG 2 cut(s) 795, 837
TseFI GTSAC 3 cut(s) 685, 694, 850
TseI GCWGC 6 cut(s) 348, 486, 605, 772, 775, 778
Tsp45I GTSAC 3 cut(s) 685, 694, 850
TspDTI ATGAA 3 cut(s) 535, 686, 722
TspGWI ACGGA 2 cut(s) 411, 611
TspRI CASTG 2 cut(s) 795, 837
Tth111I GACNNNGTC 1 cut(s) 266
VpaK11BI GGWCC 2 cut(s) 616, 754
XagI CCTNNNNNAGG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.