pycom10g22260

Pterin 4 alpha carbinolamine dehydratase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008820
Physical Location & Seq
Reverse (-)
24676199 .. 24677254
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 327 bp
ATGCGACCCATGACTGAACAAGCAGCAAATGGATTAATCACAAAGGTGGACGGATGGAATTTGGTGAGTGAAGGTGATGCTTTGAGACTGAAAAGATCATGGACAGTTAAGACCTTCACAAAAGGACTGGATATGCTTAAGCTTGTAGCTGATGTTGCAGAAGCAGAAGGTCATTATCCAGATCTTCATCTAGTTGGATGGAACAATGTAACAGTCGAGATTTGGACACATGCGGTTGGTGGATTGACCGAGAATGACTTCATACTTGCTGCTAAGATCAACAGAATCAGCATGCAACACCTACTGAGGCGGAAAGCTACCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
Pterin_4a

Protein Analysis

109

Amino Acids

12.11

Weight (kDa)

6.91

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pterin_4a PF01329 3 - 96 1.6e-26 Pterin 4 alpha carbinolamine dehydratase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 233, 310
AcsI RAATTY 1 cut(s) 58
AflII CTTAAG 1 cut(s) 137
AleI CACNNNNGTG 1 cut(s) 44
AluBI AGCT 3 cut(s) 142, 149, 317
AluI AGCT 3 cut(s) 142, 149, 317
Alw26I GTCTC 1 cut(s) 79
ApeKI GCWGC 2 cut(s) 23, 269
ApoI RAATTY 1 cut(s) 58
AseI ATTAAT 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 257
AsuHPI GGTGA 2 cut(s) 76, 86
BbvI GCAGC 2 cut(s) 35, 256
BccI CCATC 2 cut(s) 48, 192
BcoDI GTCTC 1 cut(s) 79
BfaI CTAG 1 cut(s) 191
BfrI CTTAAG 1 cut(s) 137
BglII AGATCT 1 cut(s) 181
BisI GCNGC 2 cut(s) 24, 270
BlsI GCNGC 2 cut(s) 25, 271
BmsI GCATC 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 186
Bse1I ACTGG 1 cut(s) 132
Bse8I GATNNNNATC 1 cut(s) 186
BseGI GGATG 2 cut(s) 59, 203
BseJI GATNNNNATC 1 cut(s) 186
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 1 cut(s) 132
BseXI GCAGC 2 cut(s) 35, 256
BsmAI GTCTC 1 cut(s) 79
Bsp143I GATC 3 cut(s) 95, 181, 276
BspACI CCGC 2 cut(s) 233, 310
BspCNI CTCAG 1 cut(s) 297
BspTI CTTAAG 1 cut(s) 137
BsrI ACTGG 1 cut(s) 132
BssMI GATC 3 cut(s) 95, 181, 276
Bst4CI ACNGT 2 cut(s) 106, 214
BstAFI CTTAAG 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 293
BstDEI CTNAG 2 cut(s) 273, 305
BstF5I GGATG 2 cut(s) 59, 203
BstKTI GATC 3 cut(s) 98, 184, 279
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 3 cut(s) 95, 181, 276
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNSI RCATGY 2 cut(s) 233, 295
BstV1I GCAGC 2 cut(s) 35, 256
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BtsCI GGATG 2 cut(s) 59, 203
Cac8I GCNNGC 1 cut(s) 293
CviAII CATG 4 cut(s) 10, 99, 230, 292
CviJI RGCY 3 cut(s) 142, 149, 317
CviKI_1 RGCY 3 cut(s) 142, 149, 317
DdeI CTNAG 2 cut(s) 273, 305
DpnI GATC 3 cut(s) 97, 183, 278
DpnII GATC 3 cut(s) 95, 181, 276
EciI GGCGGA 1 cut(s) 325
FaeI CATG 4 cut(s) 13, 102, 233, 295
FaiI YATR 6 cut(s) 11, 100, 134, 231, 263, 293
FatI CATG 4 cut(s) 9, 98, 229, 291
Fnu4HI GCNGC 2 cut(s) 24, 270
FokI GGATG 2 cut(s) 66, 210
Fsp4HI GCNGC 2 cut(s) 24, 270
FspBI CTAG 1 cut(s) 191
GluI GCNGC 2 cut(s) 24, 270
Hin1II CATG 4 cut(s) 13, 102, 233, 295
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 1 cut(s) 285
HphI GGTGA 2 cut(s) 76, 86
Hpy166II GTNNAC 1 cut(s) 49
Hpy188III TCNNGA 2 cut(s) 179, 217
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 3 cut(s) 65, 124, 161
HpyCH4III ACNGT 2 cut(s) 106, 214
HpyCH4V TGCA 2 cut(s) 158, 295
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 2 cut(s) 273, 305
Hsp92II CATG 4 cut(s) 13, 102, 233, 295
Kzo9I GATC 3 cut(s) 95, 181, 276
LpnPI CCDG 2 cut(s) 113, 192
Lsp1109I GCAGC 2 cut(s) 35, 256
LweI GCATC 1 cut(s) 67
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 208
MalI GATC 3 cut(s) 97, 183, 278
MboI GATC 3 cut(s) 95, 181, 276
MboII GAAGA 1 cut(s) 176
MflI RGATCY 1 cut(s) 181
MluCI AATT 1 cut(s) 58
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 1 cut(s) 300
MroXI GAANNNNTTC 1 cut(s) 257
MseI TTAA 3 cut(s) 35, 108, 138
MslI CAYNNNNRTG 1 cut(s) 44
MspCI CTTAAG 1 cut(s) 137
MwoI GCNNNNNNNGC 1 cut(s) 155
NdeII GATC 3 cut(s) 95, 181, 276
NlaIII CATG 4 cut(s) 13, 102, 233, 295
NspI RCATGY 2 cut(s) 233, 295
OliI CACNNNNGTG 1 cut(s) 44
PaeI GCATGC 1 cut(s) 295
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 1 cut(s) 285
PkrI GCNGC 2 cut(s) 25, 271
PshBI ATTAAT 1 cut(s) 35
PsuI RGATCY 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 44
SaqAI TTAA 3 cut(s) 35, 108, 138
SatI GCNGC 2 cut(s) 24, 270
Sau3AI GATC 3 cut(s) 95, 181, 276
SetI ASST 8 cut(s) 48, 76, 116, 144, 151, 172, 303, 319
SfaNI GCATC 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SphI GCATGC 1 cut(s) 295
Sse9I AATT 1 cut(s) 58
SsiI CCGC 2 cut(s) 233, 310
SspMI CTAG 1 cut(s) 191
TaaI ACNGT 2 cut(s) 106, 214
TaqI TCGA 1 cut(s) 216
TaqII GACCGA 1 cut(s) 263
TasI AATT 1 cut(s) 58
TfiI GAWTC 1 cut(s) 285
Tru1I TTAA 3 cut(s) 35, 108, 138
Tru9I TTAA 3 cut(s) 35, 108, 138
TseI GCWGC 2 cut(s) 23, 269
TspDTI ATGAA 2 cut(s) 176, 250
TspGWI ACGGA 1 cut(s) 66
Vha464I CTTAAG 1 cut(s) 137
VspI ATTAAT 1 cut(s) 35
XapI RAATTY 1 cut(s) 58
XceI RCATGY 2 cut(s) 233, 295
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.