pycom10g23130

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013321
Physical Location & Seq
Reverse (-)
25363187 .. 25364760
1574 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 933 bp
ATGGATGCCGGGGCATGTGATTTGCAGGATAGGAGGGAGGTATTGGGTGGAGTTGATCAAAAGGGGAGCGAAAAGTCGAAAAGTTCTGTTGTTTGGTCGGTTGTCGAGACTGTTATTGTGATTGAATCCGAAGAGGAAGTTCGTGTCACCGGAAAAAATGAAGATTTGGAAGCATTGGGGAGTGAGTTGGGGTCTGATAAAGCAGAGGTAGAAGGGCTTGAGAAGCCGAATGATAAGGAGGCGTGCGGCCAGCGTGGTAGTGATGAGGTACCTAGTCAAGTTGAGCAAGGAGCTAATAAGAATTCGAACAGTTCGGTTGATGGGAATGTATCTGAGACCGTGGTTGTCATTAATTCAAGTGAGTCTACGAGTGCAGTTGGAGATAACAGAAGATTGGTTCCGACGGTTGAGGAATTAGGGTCGAGTAAGGTATTAATGGAAGAGTCAAAGAAGAAGGTGCCTCAAGCAGAAAAGGATTCATATGTGATTGATATGAAGTGTGGGAGTGGTAAAGGGTTTGGTAATAAATGGGACGGGGAAAGGGTCTGTAGGATTTGCCATCTAAATTCTGATATCTCACCGCCGAGAAAAATGGGAACTGCTAATAGTTCTATCACAGCAGATTTAATTCAACTCGGTTGTGGGTGTAAAGATGAGCTTGGCGTTGCTCATGGTCATTGTGCCGAAGCATGGTTTAGGGTAAAAGGAAACAGGACGTGTGAAATATGTGGAGAGACCGCGCAAAACATTACTGGTCATGGGAATAACAGATTTATGGAGGAGTGGCATGAAGCAACGCTTGCTGATATCGGTAATAACTCATCGGAGAGGAATGTATCCTGTTGGCGAGGCCAACCATTTTGCAACTTCATAATAGCATGTTTGGTGATAGCTTTTGTACTTCCATGGTTTTTTCGTGTGAATCTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

311

Amino Acids

33.68

Weight (kDa)

4.91

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 183 - 243 2.7e-06 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013321)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34200 AT2G34200
fragaria_vesca FvH4_3g07460
malus_domestica MD10G1277000.v1.1
prunus_persica Prupe.4G064900_v2.0.a1 Prupe.4G064900_v2.0.a1
pyrus_communis pycom05g27540 pycom10g23130
rosa_chinensis RchiOBHm_Chr5g0011391
rosa_laevigata RLG00000031829
rosa_multiflora Rmu_sc0009975.1_g000013
rosa_roxburghii Rroxscaffold_1G00065060
rosa_rugosa Rorug04G0456800
rosa_samantha Rh5AG088500 Rh5BG083600 Rh5CG096900 Rh5DG083500
rosa_wichuraiana Rw5G007680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 268
AccB1I GGYRCC 2 cut(s) 268, 457
AccI GTMKAC 1 cut(s) 365
AccII CGCG 1 cut(s) 740
AciI CCGC 3 cut(s) 246, 581, 738
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 2 cut(s) 301, 565
AfaI GTAC 2 cut(s) 270, 900
AfiI CCNNNNNNNGG 1 cut(s) 690
AflIII ACRYGT 1 cut(s) 716
AgsI TTSAA 3 cut(s) 125, 357, 632
AjiI CACGTC 1 cut(s) 717
AluBI AGCT 3 cut(s) 293, 658, 893
AluI AGCT 3 cut(s) 293, 658, 893
Alw26I GTCTC 3 cut(s) 101, 329, 728
AoxI GGCC 2 cut(s) 247, 850
ApoI RAATTY 2 cut(s) 301, 565
AseI ATTAAT 2 cut(s) 351, 434
Asp700I GAANNNNTTC 1 cut(s) 926
Asp718I GGTACC 1 cut(s) 268
AspLEI GCGC 1 cut(s) 742
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 3 cut(s) 139, 570, 898
AsuII TTCGAA 1 cut(s) 305
BanI GGYRCC 2 cut(s) 268, 457
BccI CCATC 2 cut(s) 314, 567
BciVI GTATCC 1 cut(s) 847
BclI TGATCA 1 cut(s) 55
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 3 cut(s) 101, 329, 728
BfaI CTAG 2 cut(s) 273, 931
BfmI CTRYAG 1 cut(s) 547
BfuI GTATCC 1 cut(s) 847
BisI GCNGC 1 cut(s) 247
BlsI GCNGC 1 cut(s) 248
Bme1390I CCNGG 1 cut(s) 10
BmgBI CACGTC 1 cut(s) 717
BmiI GGNNCC 3 cut(s) 270, 399, 459
BmrFI CCNGG 1 cut(s) 10
Bpu14I TTCGAA 1 cut(s) 305
BpuEI CTTGAG 2 cut(s) 239, 447
BpuMI CCSGG 1 cut(s) 10
BsaI GGTCTC 2 cut(s) 329, 728
BsaJI CCNNGG 3 cut(s) 9, 339, 905
BsaWI WCCGGW 1 cut(s) 149
Bsc4I CCNNNNNNNGG 1 cut(s) 690
Bse1I ACTGG 1 cut(s) 757
BseDI CCNNGG 3 cut(s) 9, 339, 905
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 690
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 757
BseRI GAGGAG 1 cut(s) 794
BsgI GTGCAG 1 cut(s) 393
Bsh1236I CGCG 1 cut(s) 740
BshFI GGCC 2 cut(s) 249, 852
BshNI GGYRCC 2 cut(s) 268, 457
BsiSI CCGG 2 cut(s) 9, 150
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 1 cut(s) 690
BsmAI GTCTC 3 cut(s) 101, 329, 728
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 2 cut(s) 249, 852
Bso31I GGTCTC 2 cut(s) 329, 728
Bsp119I TTCGAA 1 cut(s) 305
Bsp143I GATC 1 cut(s) 55
Bsp19I CCATGG 1 cut(s) 905
BspACI CCGC 3 cut(s) 246, 581, 738
BspANI GGCC 2 cut(s) 249, 852
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 1 cut(s) 740
BspLI GGNNCC 3 cut(s) 270, 399, 459
BspT104I TTCGAA 1 cut(s) 305
BspT107I GGYRCC 2 cut(s) 268, 457
BspTNI GGTCTC 2 cut(s) 329, 728
BsrI ACTGG 1 cut(s) 757
BssECI CCNNGG 3 cut(s) 9, 339, 905
BssMI GATC 1 cut(s) 55
BssT1I CCWWGG 1 cut(s) 905
Bst4CI ACNGT 4 cut(s) 112, 311, 340, 406
Bst6I CTCTTC 2 cut(s) 126, 435
BstAPI GCANNNNNTGC 1 cut(s) 800
BstBI TTCGAA 1 cut(s) 305
BstC8I GCNNGC 3 cut(s) 244, 251, 801
BstDEI CTNAG 1 cut(s) 333
BstDSI CCRYGG 2 cut(s) 339, 905
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 740
BstHHI GCGC 1 cut(s) 742
BstKTI GATC 1 cut(s) 58
BstMAI GTCTC 3 cut(s) 101, 329, 728
BstMBI GATC 1 cut(s) 55
BstMWI GCNNNNNNNGC 2 cut(s) 223, 800
BstNSI RCATGY 2 cut(s) 18, 882
BstSCI CCNGG 1 cut(s) 8
BstSFI CTRYAG 1 cut(s) 547
BstUI CGCG 1 cut(s) 740
BsuI GTATCC 1 cut(s) 847
BsuRI GGCC 2 cut(s) 249, 852
BtgI CCRYGG 2 cut(s) 339, 905
BtrI CACGTC 1 cut(s) 717
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 3 cut(s) 244, 251, 801
CfoI GCGC 1 cut(s) 742
Csp6I GTAC 2 cut(s) 269, 899
CviAII CATG 7 cut(s) 15, 671, 690, 758, 788, 879, 906
CviJI RGCY 7 cut(s) 217, 226, 249, 293, 658, 852, 893
CviKI_1 RGCY 7 cut(s) 217, 226, 249, 293, 658, 852, 893
CviQI GTAC 2 cut(s) 269, 899
DdeI CTNAG 1 cut(s) 333
DpnI GATC 1 cut(s) 57
DpnII GATC 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 2 cut(s) 126, 435
EarI CTCTTC 2 cut(s) 126, 435
Eco130I CCWWGG 1 cut(s) 905
Eco31I GGTCTC 2 cut(s) 329, 728
Eco32I GATATC 2 cut(s) 574, 808
EcoRI GAATTC 1 cut(s) 301
EcoRV GATATC 2 cut(s) 574, 808
EcoT14I CCWWGG 1 cut(s) 905
ErhI CCWWGG 1 cut(s) 905
FaeI CATG 7 cut(s) 18, 674, 693, 761, 791, 882, 909
FalI AAGNNNNNCTT 4 cut(s) 642, 674, 783, 815
FaqI GGGAC 1 cut(s) 545
FatI CATG 7 cut(s) 14, 670, 689, 757, 787, 878, 905
FauNDI CATATG 1 cut(s) 481
FbaI TGATCA 1 cut(s) 55
FblI GTMKAC 1 cut(s) 365
Fnu4HI GCNGC 1 cut(s) 247
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 247
FspBI CTAG 2 cut(s) 273, 931
GlaI GCGC 1 cut(s) 741
GluI GCNGC 1 cut(s) 247
HaeIII GGCC 2 cut(s) 249, 852
HapII CCGG 2 cut(s) 9, 150
HhaI GCGC 1 cut(s) 742
Hin1II CATG 7 cut(s) 18, 674, 693, 761, 791, 882, 909
Hin6I GCGC 1 cut(s) 740
HinP1I GCGC 1 cut(s) 740
HinfI GANTC 5 cut(s) 125, 362, 443, 476, 922
HpaII CCGG 2 cut(s) 9, 150
HphI GGTGA 3 cut(s) 139, 570, 898
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 6 cut(s) 130, 196, 334, 402, 571, 826
Hpy188III TCNNGA 1 cut(s) 106
Hpy8I GTNNAC 1 cut(s) 366
Hpy99I CGWCG 1 cut(s) 406
HpyAV CCTTC 2 cut(s) 206, 448
HpyCH4III ACNGT 4 cut(s) 112, 311, 340, 406
HpyCH4IV ACGT 1 cut(s) 716
HpyCH4V TGCA 3 cut(s) 25, 374, 864
HpyF10VI GCNNNNNNNGC 2 cut(s) 223, 800
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 1 cut(s) 716
Hsp92II CATG 7 cut(s) 18, 674, 693, 761, 791, 882, 909
HspAI GCGC 1 cut(s) 740
KpnI GGTACC 1 cut(s) 272
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 1 cut(s) 55
LmnI GCTCC 2 cut(s) 66, 290
LpnPI CCDG 7 cut(s) 11, 22, 163, 263, 697, 738, 853
MaeI CTAG 2 cut(s) 273, 931
MaeII ACGT 1 cut(s) 716
MaeIII GTNAC 1 cut(s) 145
MalI GATC 1 cut(s) 57
MboI GATC 1 cut(s) 55
MboII GAAGA 5 cut(s) 143, 173, 402, 452, 463
MluCI AATT 5 cut(s) 301, 352, 413, 565, 627
MlyI GAGTC 2 cut(s) 371, 452
MmeI TCCRAC 2 cut(s) 358, 425
MroXI GAANNNNTTC 1 cut(s) 926
MseI TTAA 3 cut(s) 351, 434, 626
MspI CCGG 2 cut(s) 9, 150
MspR9I CCNGG 1 cut(s) 10
MvnI CGCG 1 cut(s) 740
MwoI GCNNNNNNNGC 2 cut(s) 223, 800
NciI CCSGG 1 cut(s) 10
NcoI CCATGG 1 cut(s) 905
NdeI CATATG 1 cut(s) 481
NdeII GATC 1 cut(s) 55
NlaIII CATG 7 cut(s) 18, 674, 693, 761, 791, 882, 909
NlaIV GGNNCC 3 cut(s) 270, 399, 459
NmeAIII GCCGAG 1 cut(s) 609
NmuCI GTSAC 1 cut(s) 145
NspI RCATGY 2 cut(s) 18, 882
NspV TTCGAA 1 cut(s) 305
PdmI GAANNNNTTC 1 cut(s) 926
PfeI GAWTC 3 cut(s) 125, 476, 922
PkrI GCNGC 1 cut(s) 248
PleI GAGTC 2 cut(s) 370, 451
PpsI GAGTC 2 cut(s) 370, 451
PshBI ATTAAT 2 cut(s) 351, 434
PspN4I GGNNCC 3 cut(s) 270, 399, 459
RsaI GTAC 2 cut(s) 270, 900
RsaNI GTAC 2 cut(s) 269, 899
SaqAI TTAA 3 cut(s) 351, 434, 626
SatI GCNGC 1 cut(s) 247
Sau3AI GATC 1 cut(s) 55
SchI GAGTC 2 cut(s) 371, 452
ScrFI CCNGG 1 cut(s) 10
SfcI CTRYAG 1 cut(s) 547
SfuI TTCGAA 1 cut(s) 305
SmlI CTYRAG 2 cut(s) 218, 462
SmoI CTYRAG 2 cut(s) 218, 462
Sse9I AATT 5 cut(s) 301, 352, 413, 565, 627
SsiI CCGC 3 cut(s) 246, 581, 738
SspMI CTAG 2 cut(s) 273, 931
StyD4I CCNGG 1 cut(s) 8
StyI CCWWGG 1 cut(s) 905
TaaI ACNGT 4 cut(s) 112, 311, 340, 406
TaiI ACGT 1 cut(s) 719
TaqI TCGA 4 cut(s) 77, 105, 305, 422
TasI AATT 5 cut(s) 301, 352, 413, 565, 627
TatI WGTACW 1 cut(s) 898
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 3 cut(s) 125, 476, 922
Tru1I TTAA 3 cut(s) 351, 434, 626
Tru9I TTAA 3 cut(s) 351, 434, 626
TseFI GTSAC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 5 cut(s) 174, 468, 509, 804, 859
VspI ATTAAT 2 cut(s) 351, 434
XapI RAATTY 2 cut(s) 301, 565
XceI RCATGY 2 cut(s) 18, 882
XmiI GTMKAC 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 926
XspI CTAG 2 cut(s) 273, 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.