pycom10g23180

nucleobase-ascorbate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008195
Physical Location & Seq
Reverse (-)
25397897 .. 25401310
3414 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1575 bp
ATGGAAGCTCCAAAACCAGAAGAGATAAGCCATCCAGCAATGGACCAACTCCAAGGCTTAGAGTACTGCATTGACTCAAATCCCTCTTGGGCGGAAGCAATTGCTTTGGGTTTTCAGCACTACATTTTGGCCTTGGGAACTGCTGTGATGATCCCCACATTCCTTGTTCCTTTCATGGGTGGTGATGATGGTGACAAAGTGAGAGTAGTGCAGACTCTCCTTTTTATTCAAGGAATCAACACACTTCTACAAACATTGTTTGGAACCCGTTTGCCAACCGTGATTGGAGGGTCTTACGCATTCATGGTCCCGATAATATCCATAATTCATGATTCCCGCTTGATGAAGATTGAGGACAATCATGCGAGATTCCTTGACACCATGAGAGCAGTCCAAGGGGCTCTGATAGTAGCTTCAAGCATACAAATTATTCTGGGATATAGTCAAATTTGGGCCATCTGTTCCAGGTTCTTCAGCCCGCTCGGAATGGTTCCAGTTATTTCCTTGGTGGGTTTTGGTCTGTTTGATAGAGGCTTCCCCCTGGTTGGAAAGTGTGTGGAAATTGGCATTCCTATGCTTATTCTTTTTGTAGCTTTCTCTCAGTACCTAAAGAACTTCCATGCAAGACAGCTACCAGTATTGGAACGATTTGCTCTTCTTATATCAGTCACAGTGATATGGGCATATGCACACCTCTTGACCGCCAGTGGTGCATACAGACACCGCCCAGACATAACCCAAGTCAATTGCCGAACTGATAGGGCCCACCTCATTTCTTCTGCTCCATGGATAAAAATACCTTATCCTCTTCAATGGGGTGCCCCAACCTTTGATGCTGGTCATGCTTTTGGAATGATGGCTGCCGTTTTTGTTTCATTGATCGAGTCAACTGGAGCATACAAGGCTGCATCGCGTCTAGCAAGTGCCACAATACCTCCAGCTCATGTTCTCAGCCGTGGAATTGGCTGGCAGGGAATAGGAATCCTACTAAATGGACTCTTTGGGACGTTGTCAGGCTCAACAGTCTCTATAGAAAATGTGGGGCTTCTTGGGAGCACTCGTGTTGGAAGCCGTAGGGTCATTCAAATCTCAGCCGGTTTTATGATATTCTTCTCCATGTTAGGTAAATTTGGAGCTCTGTTCGCATCAATACCCTTCACCATATTTGCTGCTGCCTATTGTGTATTGTTCGGTCTTGTTGCTTCGGTGGGGTTATCCTTTTTGCAATTCACCAACATGAACTCGACGAGGAACCTCTTTATTGTTGGTGTAGCTTTCTTCTTGGGTTTGTCTGTTCCCGAGTACTTCAGGGGTTACACTTTGAAGGCTTTCCATGGTCCTGCTCATACCAATGCCGGATGGTTCAATGATTTTCTGAATACCATCTTCTCCTCATCTCCAACTGTGGCATTGATTGTCGCCGTTTTCCTGGATAACACACTTGAGTACAAGGACAGTGCAAGAGACAGAGGAATGCCGTGGTGGGTCAAATTTCGGCAATTCAAAGGGGACAGCCGCAACGAGGAGTTTTACACTCTCCCTTTCAACCTTAACCGATTCTTCCCTCCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

525

Amino Acids

57.67

Weight (kDa)

9.02

Isoelectric Point (pI)

30.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 33 - 436 2.1e-62 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 818
AccBSI CCGCTC 1 cut(s) 481
AccII CGCG 1 cut(s) 913
AciI CCGC 6 cut(s) 92, 337, 479, 702, 724, 1516
AclWI GGATC 1 cut(s) 145
AcsI RAATTY 3 cut(s) 447, 1127, 1490
AcuI CTGAAG 2 cut(s) 457, 1291
AfaI GTAC 4 cut(s) 65, 605, 1304, 1448
AfiI CCNNNNNNNGG 3 cut(s) 176, 545, 1522
AgsI TTSAA 8 cut(s) 230, 417, 812, 1085, 1324, 1366, 1504, 1546
AjnI CCWGG 3 cut(s) 464, 540, 1428
AluBI AGCT 7 cut(s) 8, 413, 593, 631, 941, 1136, 1274
AluI AGCT 7 cut(s) 8, 413, 593, 631, 941, 1136, 1274
Alw21I GWGCWC 2 cut(s) 1058, 1138
Alw26I GTCTC 2 cut(s) 1030, 1458
AlwI GGATC 1 cut(s) 145
Ama87I CYCGRG 1 cut(s) 1298
AoxI GGCC 3 cut(s) 129, 453, 762
ApaI GGGCCC 1 cut(s) 766
ApeKI GCWGC 4 cut(s) 860, 905, 1169, 1172
ApoI RAATTY 3 cut(s) 447, 1127, 1490
Asp700I GAANNNNTTC 1 cut(s) 1328
AspS9I GGNCC 6 cut(s) 43, 307, 453, 762, 763, 1337
AsuHPI GGTGA 4 cut(s) 194, 203, 1150, 1222
AvaI CYCGRG 1 cut(s) 1298
AvaII GGWCC 3 cut(s) 43, 307, 1337
BaeGI GKGCMC 2 cut(s) 766, 823
BanI GGYRCC 1 cut(s) 818
BanII GRGCYC 3 cut(s) 403, 766, 1138
BauI CACGAG 1 cut(s) 1059
Bbv12I GWGCWC 2 cut(s) 1058, 1138
BbvI GCAGC 4 cut(s) 847, 892, 1156, 1159
BccI CCATC 6 cut(s) 39, 182, 464, 850, 1353, 1391
BceAI ACGGC 5 cut(s) 848, 939, 1056, 1406, 1462
BciT130I CCWGG 3 cut(s) 466, 542, 1430
BcoDI GTCTC 2 cut(s) 1030, 1458
BfaI CTAG 1 cut(s) 917
BfmI CTRYAG 1 cut(s) 1029
BisI GCNGC 5 cut(s) 861, 906, 1170, 1173, 1516
BlsI GCNGC 5 cut(s) 862, 907, 1171, 1174, 1517
BmcAI AGTACT 2 cut(s) 65, 1304
Bme1390I CCNGG 3 cut(s) 466, 542, 1430
Bme18I GGWCC 3 cut(s) 43, 307, 1337
BmeT110I CYCGRG 1 cut(s) 1298
BmgT120I GGNCC 6 cut(s) 43, 307, 453, 762, 763, 1337
BmiI GGNNCC 6 cut(s) 265, 309, 492, 764, 820, 1253
BmrFI CCNGG 3 cut(s) 466, 542, 1430
BmsI GCATC 3 cut(s) 823, 917, 1154
BpmI CTGGAG 2 cut(s) 912, 921
BpuEI CTTGAG 1 cut(s) 1463
BsaJI CCNNGG 9 cut(s) 52, 132, 394, 504, 540, 785, 955, 1333, 1478
BsaXI ACNNNNNCTCC 4 cut(s) 919, 949, 1045, 1075
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 545, 1522
Bse118I RCCGGY 1 cut(s) 1094
Bse1I ACTGG 4 cut(s) 494, 635, 705, 895
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 3 cut(s) 466, 542, 1430
BseDI CCNNGG 9 cut(s) 52, 132, 394, 504, 540, 785, 955, 1333, 1478
BseGI GGATG 2 cut(s) 31, 1364
BseLI CCNNNNNNNGG 3 cut(s) 176, 545, 1522
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 3 cut(s) 614, 964, 1104
BseNI ACTGG 4 cut(s) 494, 635, 705, 895
BseRI GAGGAG 2 cut(s) 1381, 1538
BseSI GKGCMC 2 cut(s) 766, 823
BseXI GCAGC 4 cut(s) 847, 892, 1156, 1159
BsgI GTGCAG 1 cut(s) 230
Bsh1236I CGCG 1 cut(s) 913
BshFI GGCC 3 cut(s) 131, 455, 764
BshNI GGYRCC 1 cut(s) 818
BsiHKAI GWGCWC 2 cut(s) 1058, 1138
BsiHKCI CYCGRG 1 cut(s) 1298
BsiSI CCGG 2 cut(s) 1095, 1356
BslFI GGGAC 3 cut(s) 293, 1018, 1523
BslI CCNNNNNNNGG 3 cut(s) 176, 545, 1522
BsmAI GTCTC 2 cut(s) 1030, 1458
BsmFI GGGAC 3 cut(s) 293, 1018, 1523
BsmI GAATGC 3 cut(s) 299, 567, 1479
BsnI GGCC 3 cut(s) 131, 455, 764
BsoBI CYCGRG 1 cut(s) 1298
Bsp120I GGGCCC 1 cut(s) 762
Bsp1286I GDGCHC 5 cut(s) 403, 766, 823, 1058, 1138
Bsp143I GATC 2 cut(s) 150, 879
Bsp19I CCATGG 2 cut(s) 785, 1333
BspACI CCGC 6 cut(s) 92, 337, 479, 702, 724, 1516
BspANI GGCC 3 cut(s) 131, 455, 764
BspCNI CTCAG 3 cut(s) 613, 963, 1103
BspFNI CGCG 1 cut(s) 913
BspHI TCATGA 1 cut(s) 328
BspLI GGNNCC 6 cut(s) 265, 309, 492, 764, 820, 1253
BspPI GGATC 1 cut(s) 145
BspQI GCTCTTC 1 cut(s) 660
BspT107I GGYRCC 1 cut(s) 818
BsrBI CCGCTC 1 cut(s) 481
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 1094
BsrI ACTGG 4 cut(s) 494, 635, 705, 895
BssAI RCCGGY 1 cut(s) 1094
BssECI CCNNGG 9 cut(s) 52, 132, 394, 504, 540, 785, 955, 1333, 1478
BssMI GATC 2 cut(s) 150, 879
BssSI CACGAG 1 cut(s) 1059
BssT1I CCWWGG 6 cut(s) 52, 132, 394, 504, 785, 1333
Bst2BI CACGAG 1 cut(s) 1059
Bst2UI CCWGG 3 cut(s) 466, 542, 1430
Bst4CI ACNGT 5 cut(s) 280, 673, 1024, 1405, 1457
Bst6I CTCTTC 3 cut(s) 15, 660, 813
BstC8I GCNNGC 2 cut(s) 479, 968
BstDEI CTNAG 4 cut(s) 58, 600, 950, 1090
BstDSI CCRYGG 4 cut(s) 785, 955, 1333, 1478
BstF5I GGATG 2 cut(s) 31, 1364
BstFNI CGCG 1 cut(s) 913
BstKTI GATC 2 cut(s) 153, 882
BstMAI GTCTC 2 cut(s) 1030, 1458
BstMBI GATC 2 cut(s) 150, 879
BstMWI GCNNNNNNNGC 4 cut(s) 710, 842, 902, 1142
BstNI CCWGG 3 cut(s) 466, 542, 1430
BstSCI CCNGG 3 cut(s) 464, 540, 1428
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 2 cut(s) 766, 823
BstUI CGCG 1 cut(s) 913
BstV1I GCAGC 4 cut(s) 847, 892, 1156, 1159
BsuRI GGCC 3 cut(s) 131, 455, 764
BtgI CCRYGG 4 cut(s) 785, 955, 1333, 1478
BtgZI GCGATG 1 cut(s) 894
BtsCI GGATG 2 cut(s) 31, 1364
BtsIMutI CAGTG 3 cut(s) 678, 712, 1462
Cac8I GCNNGC 2 cut(s) 479, 968
CciI TCATGA 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 1094
Cfr13I GGNCC 6 cut(s) 43, 307, 453, 762, 763, 1337
CseI GACGC 1 cut(s) 902
Csp6I GTAC 4 cut(s) 64, 604, 1303, 1447
CspCI CAANNNNNGTGG 2 cut(s) 145, 180
CviQI GTAC 4 cut(s) 64, 604, 1303, 1447
DdeI CTNAG 4 cut(s) 58, 600, 950, 1090
DpnI GATC 2 cut(s) 152, 881
DpnII GATC 2 cut(s) 150, 879
Eam1104I CTCTTC 3 cut(s) 15, 660, 813
EarI CTCTTC 3 cut(s) 15, 660, 813
EciI GGCGGA 1 cut(s) 107
Ecl136II GAGCTC 1 cut(s) 1136
Eco130I CCWWGG 6 cut(s) 52, 132, 394, 504, 785, 1333
Eco24I GRGCYC 3 cut(s) 403, 766, 1138
Eco47I GGWCC 3 cut(s) 43, 307, 1337
Eco53kI GAGCTC 1 cut(s) 1136
Eco57I CTGAAG 2 cut(s) 457, 1291
Eco88I CYCGRG 1 cut(s) 1298
EcoICRI GAGCTC 1 cut(s) 1136
EcoO109I RGGNCCY 1 cut(s) 762
EcoRII CCWGG 3 cut(s) 464, 540, 1428
EcoT14I CCWWGG 6 cut(s) 52, 132, 394, 504, 785, 1333
EcoT38I GRGCYC 3 cut(s) 403, 766, 1138
ErhI CCWWGG 6 cut(s) 52, 132, 394, 504, 785, 1333
FaqI GGGAC 3 cut(s) 293, 1018, 1523
FauI CCCGC 2 cut(s) 344, 486
FauNDI CATATG 1 cut(s) 685
Fnu4HI GCNGC 5 cut(s) 861, 906, 1170, 1173, 1516
FokI GGATG 2 cut(s) 18, 1371
FriOI GRGCYC 3 cut(s) 403, 766, 1138
Fsp4HI GCNGC 5 cut(s) 861, 906, 1170, 1173, 1516
FspBI CTAG 1 cut(s) 917
GluI GCNGC 5 cut(s) 861, 906, 1170, 1173, 1516
GsuI CTGGAG 2 cut(s) 912, 921
HaeIII GGCC 3 cut(s) 131, 455, 764
HapII CCGG 2 cut(s) 1095, 1356
HgaI GACGC 1 cut(s) 902
HincII GTYRAC 1 cut(s) 888
HindII GTYRAC 1 cut(s) 888
HinfI GANTC 9 cut(s) 74, 214, 234, 332, 369, 884, 981, 996, 1557
HpaII CCGG 2 cut(s) 1095, 1356
HphI GGTGA 4 cut(s) 194, 203, 1150, 1222
Hpy166II GTNNAC 1 cut(s) 888
Hpy188I TCNGA 3 cut(s) 405, 485, 1377
Hpy188III TCNNGA 4 cut(s) 310, 329, 697, 1298
Hpy8I GTNNAC 1 cut(s) 888
Hpy99I CGWCG 1 cut(s) 1249
HpyAV CCTTC 2 cut(s) 1165, 1318
HpyCH4III ACNGT 5 cut(s) 280, 673, 1024, 1405, 1457
HpyCH4IV ACGT 1 cut(s) 1007
HpyCH4V TGCA 8 cut(s) 69, 211, 623, 689, 713, 908, 1225, 1460
HpyF10VI GCNNNNNNNGC 4 cut(s) 710, 842, 902, 1142
HpyF3I CTNAG 4 cut(s) 58, 600, 950, 1090
HpySE526I ACGT 1 cut(s) 1007
Kzo9I GATC 2 cut(s) 150, 879
LguI GCTCTTC 1 cut(s) 660
LmnI GCTCC 5 cut(s) 13, 787, 893, 1053, 1133
Lsp1109I GCAGC 4 cut(s) 847, 892, 1156, 1159
LweI GCATC 3 cut(s) 823, 917, 1154
MaeI CTAG 1 cut(s) 917
MaeII ACGT 1 cut(s) 1007
MaeIII GTNAC 3 cut(s) 191, 667, 1313
MalI GATC 2 cut(s) 152, 881
MbiI CCGCTC 1 cut(s) 481
MboI GATC 2 cut(s) 150, 879
MfeI CAATTG 2 cut(s) 99, 745
MhlI GDGCHC 5 cut(s) 403, 766, 823, 1058, 1138
MlyI GAGTC 4 cut(s) 68, 208, 893, 990
MmeI TCCRAC 3 cut(s) 526, 1045, 1424
MroXI GAANNNNTTC 1 cut(s) 1328
MseI TTAA 2 cut(s) 1551, 1573
MslI CAYNNNNRTG 3 cut(s) 572, 1235, 1350
MspI CCGG 2 cut(s) 1095, 1356
MspR9I CCNGG 3 cut(s) 466, 542, 1430
MunI CAATTG 2 cut(s) 99, 745
Mva1269I GAATGC 3 cut(s) 299, 567, 1479
MvaI CCWGG 3 cut(s) 466, 542, 1430
MvnI CGCG 1 cut(s) 913
MwoI GCNNNNNNNGC 4 cut(s) 710, 842, 902, 1142
NcoI CCATGG 2 cut(s) 785, 1333
NdeI CATATG 1 cut(s) 685
NdeII GATC 2 cut(s) 150, 879
NlaIV GGNNCC 6 cut(s) 265, 309, 492, 764, 820, 1253
NmuCI GTSAC 2 cut(s) 191, 667
PagI TCATGA 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 660
PctI GAATGC 3 cut(s) 299, 567, 1479
PdmI GAANNNNTTC 1 cut(s) 1328
PfeI GAWTC 5 cut(s) 234, 332, 369, 981, 1557
PflFI GACNNNGTC 1 cut(s) 1009
PfoI TCCNGGA 1 cut(s) 1428
PkrI GCNGC 5 cut(s) 862, 907, 1171, 1174, 1517
PleI GAGTC 4 cut(s) 68, 208, 892, 990
PpsI GAGTC 4 cut(s) 68, 208, 892, 990
Psp124BI GAGCTC 1 cut(s) 1138
Psp6I CCWGG 3 cut(s) 464, 540, 1428
PspGI CCWGG 3 cut(s) 464, 540, 1428
PspN4I GGNNCC 6 cut(s) 265, 309, 492, 764, 820, 1253
PspOMI GGGCCC 1 cut(s) 762
PspPI GGNCC 6 cut(s) 43, 307, 453, 762, 763, 1337
PsyI GACNNNGTC 1 cut(s) 1009
RsaI GTAC 4 cut(s) 65, 605, 1304, 1448
RsaNI GTAC 4 cut(s) 64, 604, 1303, 1447
RseI CAYNNNNRTG 3 cut(s) 572, 1235, 1350
SacI GAGCTC 1 cut(s) 1138
SapI GCTCTTC 1 cut(s) 660
SaqAI TTAA 2 cut(s) 1551, 1573
SatI GCNGC 5 cut(s) 861, 906, 1170, 1173, 1516
Sau3AI GATC 2 cut(s) 150, 879
Sau96I GGNCC 6 cut(s) 43, 307, 453, 762, 763, 1337
ScaI AGTACT 2 cut(s) 65, 1304
SchI GAGTC 4 cut(s) 68, 208, 893, 990
ScrFI CCNGG 3 cut(s) 466, 542, 1430
SduI GDGCHC 5 cut(s) 403, 766, 823, 1058, 1138
SfaNI GCATC 3 cut(s) 823, 917, 1154
SfcI CTRYAG 1 cut(s) 1029
SinI GGWCC 3 cut(s) 43, 307, 1337
SmiMI CAYNNNNRTG 3 cut(s) 572, 1235, 1350
SmlI CTYRAG 1 cut(s) 1442
SmoI CTYRAG 1 cut(s) 1442
SsiI CCGC 6 cut(s) 92, 337, 479, 702, 724, 1516
SspMI CTAG 1 cut(s) 917
SstI GAGCTC 1 cut(s) 1138
StyD4I CCNGG 3 cut(s) 464, 540, 1428
StyI CCWWGG 6 cut(s) 52, 132, 394, 504, 785, 1333
TaaI ACNGT 5 cut(s) 280, 673, 1024, 1405, 1457
TaiI ACGT 1 cut(s) 1010
TaqI TCGA 2 cut(s) 882, 1244
TaqII GACCGA 1 cut(s) 1181
TatI WGTACW 3 cut(s) 63, 1302, 1446
TauI GCSGC 1 cut(s) 1518
TfiI GAWTC 5 cut(s) 234, 332, 369, 981, 1557
Tru1I TTAA 2 cut(s) 1551, 1573
Tru9I TTAA 2 cut(s) 1551, 1573
TscAI CASTG 3 cut(s) 678, 712, 1462
TseFI GTSAC 2 cut(s) 191, 667
TseI GCWGC 4 cut(s) 860, 905, 1169, 1172
Tsp45I GTSAC 2 cut(s) 191, 667
TspDTI ATGAA 6 cut(s) 163, 292, 317, 359, 864, 1253
TspRI CASTG 3 cut(s) 678, 712, 1462
Tth111I GACNNNGTC 1 cut(s) 1009
VpaK11BI GGWCC 3 cut(s) 43, 307, 1337
XapI RAATTY 3 cut(s) 447, 1127, 1490
XmnI GAANNNNTTC 1 cut(s) 1328
XspI CTAG 1 cut(s) 917
ZrmI AGTACT 2 cut(s) 65, 1304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.