pycom10g24490

ralf-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003104
Physical Location & Seq
Forward (+)
26333068 .. 26333433
366 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 366 bp
ATGGGATTCAAGCTCTACCTGATCGCCTTCATGGTGGTATTGGCCATGGTGGCTAGGTCATCAGCCAAGGTCCACGATGCCTCGTGGGGTGTCAGCCACTTCGTTGCTCATGATGATGAATCCTTGATCGACAGCACCAACAGCAAAATTGGCGACGTCGGAGAGGAGAATGAGTTGTTGATGGATTCAAACAGCGGTCGTAGGACACTCCAAGGCAACACCAGGTATATTGGCTATGGTGCCCTGAGGCGCAATGCTGTTCCATGCGGACGCCGCGGCCAGTCCTACTACAACTGCCAAAGAAAGGAGGGAGCTAACCCTTACAAGCGCGGTTGCGAGTACATCACCCGCTGCTCCAGACGCTAA

Protein Analysis

122

Amino Acids

13.6

Weight (kDa)

9.35

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 58 - 120 4.6e-25 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 159
AccB1I GGYRCC 1 cut(s) 239
AccII CGCG 2 cut(s) 276, 330
AciI CCGC 6 cut(s) 195, 267, 274, 276, 330, 349
AcoI YGGCCR 2 cut(s) 42, 277
AcyI GRCGYC 2 cut(s) 156, 271
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 2 cut(s) 10, 189
AjnI CCWGG 1 cut(s) 221
AluBI AGCT 2 cut(s) 13, 314
AluI AGCT 2 cut(s) 13, 314
AoxI GGCC 2 cut(s) 42, 277
ApeKI GCWGC 1 cut(s) 351
AspLEI GCGC 2 cut(s) 252, 330
AspS9I GGNCC 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 337
AvaII GGWCC 1 cut(s) 70
AxyI CCTNAGG 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 244
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 239
BauI CACGAG 1 cut(s) 82
BbvI GCAGC 1 cut(s) 338
BccI CCATC 1 cut(s) 175
BciT130I CCWGG 1 cut(s) 223
BfaI CTAG 1 cut(s) 54
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 3 cut(s) 274, 277, 352
BlsI GCNGC 3 cut(s) 275, 278, 353
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 1 cut(s) 223
BmsI GCATC 1 cut(s) 67
BpmI CTGGAG 1 cut(s) 340
BsaHI GRCGYC 2 cut(s) 156, 271
BsaJI CCNNGG 4 cut(s) 45, 66, 211, 274
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse1I ACTGG 1 cut(s) 280
Bse21I CCTNAGG 1 cut(s) 245
Bse3DI GCAATG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 4 cut(s) 45, 66, 211, 274
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 280
BseRI GAGGAG 1 cut(s) 179
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 1 cut(s) 338
Bsh1236I CGCG 2 cut(s) 276, 330
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 2 cut(s) 44, 279
BshNI GGYRCC 1 cut(s) 239
BsiEI CGRYCG 1 cut(s) 199
BslI CCNNNNNNNGG 1 cut(s) 304
BsnI GGCC 2 cut(s) 44, 279
Bsp1286I GDGCHC 1 cut(s) 244
Bsp143I GATC 2 cut(s) 21, 126
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 6 cut(s) 195, 267, 274, 276, 330, 349
BspANI GGCC 2 cut(s) 44, 279
BspCNI CTCAG 1 cut(s) 237
BspFNI CGCG 2 cut(s) 276, 330
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 1 cut(s) 241
BspT107I GGYRCC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 259
BsrI ACTGG 1 cut(s) 280
BssECI CCNNGG 4 cut(s) 45, 66, 211, 274
BssMI GATC 2 cut(s) 21, 126
BssNI GRCGYC 2 cut(s) 156, 271
BssSI CACGAG 1 cut(s) 82
BssT1I CCWWGG 3 cut(s) 45, 66, 211
Bst2BI CACGAG 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 223
BstACI GRCGYC 2 cut(s) 156, 271
BstDEI CTNAG 1 cut(s) 245
BstDSI CCRYGG 2 cut(s) 45, 274
BstFNI CGCG 2 cut(s) 276, 330
BstHHI GCGC 2 cut(s) 252, 330
BstKTI GATC 2 cut(s) 24, 129
BstMBI GATC 2 cut(s) 21, 126
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 5 cut(s) 50, 141, 150, 273, 360
BstNI CCWGG 1 cut(s) 223
BstSCI CCNGG 1 cut(s) 221
BstSLI GKGCMC 1 cut(s) 244
BstUI CGCG 2 cut(s) 276, 330
BstV1I GCAGC 1 cut(s) 338
Bsu36I CCTNAGG 1 cut(s) 245
BsuRI GGCC 2 cut(s) 44, 279
BtgI CCRYGG 2 cut(s) 45, 274
CciI TCATGA 1 cut(s) 109
CfoI GCGC 2 cut(s) 252, 330
Cfr13I GGNCC 1 cut(s) 70
Cfr42I CCGCGG 1 cut(s) 277
CseI GACGC 1 cut(s) 279
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 1 cut(s) 340
CviAII CATG 4 cut(s) 31, 46, 110, 264
CviJI RGCY 8 cut(s) 13, 44, 53, 65, 96, 234, 279, 314
CviKI_1 RGCY 8 cut(s) 13, 44, 53, 65, 96, 234, 279, 314
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 1 cut(s) 245
DpnI GATC 2 cut(s) 23, 128
DpnII GATC 2 cut(s) 21, 126
EaeI YGGCCR 2 cut(s) 42, 277
Eco130I CCWWGG 3 cut(s) 45, 66, 211
Eco47I GGWCC 1 cut(s) 70
Eco81I CCTNAGG 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 221
EcoT14I CCWWGG 3 cut(s) 45, 66, 211
ErhI CCWWGG 3 cut(s) 45, 66, 211
FaeI CATG 4 cut(s) 34, 49, 113, 267
FaiI YATR 6 cut(s) 32, 47, 111, 228, 237, 265
FatI CATG 4 cut(s) 30, 45, 109, 263
FauI CCCGC 1 cut(s) 356
Fnu4HI GCNGC 3 cut(s) 274, 277, 352
Fsp4HI GCNGC 3 cut(s) 274, 277, 352
FspBI CTAG 1 cut(s) 54
GlaI GCGC 2 cut(s) 251, 329
GluI GCNGC 3 cut(s) 274, 277, 352
GsuI CTGGAG 1 cut(s) 340
HaeIII GGCC 2 cut(s) 44, 279
HgaI GACGC 1 cut(s) 279
HhaI GCGC 2 cut(s) 252, 330
Hin1I GRCGYC 2 cut(s) 156, 271
Hin1II CATG 4 cut(s) 34, 49, 113, 267
Hin6I GCGC 2 cut(s) 250, 328
HinP1I GCGC 2 cut(s) 250, 328
HinfI GANTC 3 cut(s) 6, 119, 185
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 1 cut(s) 161
Hpy188III TCNNGA 2 cut(s) 110, 357
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 2 cut(s) 158, 161
HpyAV CCTTC 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 156
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 141, 150, 273, 360
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 1 cut(s) 156
Hsp92I GRCGYC 2 cut(s) 156, 271
Hsp92II CATG 4 cut(s) 34, 49, 113, 267
HspAI GCGC 2 cut(s) 250, 328
KspI CCGCGG 1 cut(s) 277
Kzo9I GATC 2 cut(s) 21, 126
LmnI GCTCC 2 cut(s) 311, 359
LpnPI CCDG 5 cut(s) 32, 208, 235, 257, 293
Lsp1109I GCAGC 1 cut(s) 338
LweI GCATC 1 cut(s) 67
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 1 cut(s) 54
MaeII ACGT 1 cut(s) 156
MalI GATC 2 cut(s) 23, 128
MboI GATC 2 cut(s) 21, 126
MhlI GDGCHC 1 cut(s) 244
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 1 cut(s) 147
MluNI TGGCCA 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 4 cut(s) 91, 157, 240, 301
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 114
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 3 cut(s) 195, 276, 351
MspR9I CCNGG 1 cut(s) 223
MvaI CCWGG 1 cut(s) 223
MvnI CGCG 2 cut(s) 276, 330
MwoI GCNNNNNNNGC 5 cut(s) 50, 141, 150, 273, 360
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 21, 126
NlaIII CATG 4 cut(s) 34, 49, 113, 267
NlaIV GGNNCC 1 cut(s) 241
PagI TCATGA 1 cut(s) 109
PfeI GAWTC 3 cut(s) 6, 119, 185
PkrI GCNGC 3 cut(s) 275, 278, 353
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 1 cut(s) 70
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
RseI CAYNNNNRTG 1 cut(s) 114
SacII CCGCGG 1 cut(s) 277
SatI GCNGC 3 cut(s) 274, 277, 352
Sau3AI GATC 2 cut(s) 21, 126
Sau96I GGNCC 1 cut(s) 70
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 1 cut(s) 244
SetI ASST 7 cut(s) 15, 21, 59, 72, 159, 227, 316
SexAI ACCWGGT 1 cut(s) 221
SfaNI GCATC 1 cut(s) 67
Sfr303I CCGCGG 1 cut(s) 277
SgrBI CCGCGG 1 cut(s) 277
SinI GGWCC 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 114
Sse9I AATT 1 cut(s) 147
SsiI CCGC 6 cut(s) 195, 267, 274, 276, 330, 349
SspMI CTAG 1 cut(s) 54
StyD4I CCNGG 1 cut(s) 221
StyI CCWWGG 3 cut(s) 45, 66, 211
TaiI ACGT 1 cut(s) 159
TaqI TCGA 1 cut(s) 129
TasI AATT 1 cut(s) 147
TatI WGTACW 1 cut(s) 339
TauI GCSGC 2 cut(s) 276, 279
TfiI GAWTC 3 cut(s) 6, 119, 185
TseI GCWGC 1 cut(s) 351
TspDTI ATGAA 2 cut(s) 19, 132
VpaK11BI GGWCC 1 cut(s) 70
XspI CTAG 1 cut(s) 54
ZraI GACGTC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.