pycom10g24500

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012106
Physical Location & Seq
Reverse (-)
26343000 .. 26343968
969 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 969 bp
ATGTACGCATCACACTCCTCCCAAGCGCGTGAGGAGACTCCAACAACAGTAGAACCCGAACTCTACTCACCAAAACATTTCTTACCAAACAAAAAGAAAGTCTCATCTCATAAAAAACTCACTCGCTCTCTTCCTCCCTCCCGCTCCCTCTCTCTCAATTTTCTCACTTTCTCGCACTTTCTCAGCAACCAAACAGAAAATAAATCAGAGAACCCATCAATGGAATCCTCACCCAGGCACCACCAACCGGATAACCCAACTCCTTCTTTGCTTCCCTCCCCAAACAGCCACAGCTCCAACAGCAGCACAAGCAGCTCCAACAGCAACCACCACCTCAACGGCGGAGCTCCCCCAACAACCCCAACTCCCCAGCCCCCTAAACCCTACACCAGATCCGAACCCACCAACCCGTATCCGACCACCTTCGTCCAAGCTGACACCTCCTCCTTCAAACAAGTCGTTCAAATGCTCACCGGCTCCACCGAAACCGCTAAGCAAGCCTCTTCGAAATCCACATCCGACCCGCACGCGAAAACACACATCCCGCCCATCAAATCCACGCACACAAAACAACAATCCGGGTCCGGGTTCAAGCTCTACGAGCGCCGGAACTCCCTCAAGAACCTCCGGATTAACCCTCTAATCCCCATCTTTAGCTCCGCAGCTTCCGGATTCTCGCCGCGAAATCCCGAGATTTTATCTCCGAGCATTCTCGACTTTCCTGCTTTGGTTCTCAGCCCTGTCACGCCGCTGATATCCGACCCGTTTGACCGGTCCGGGACGGCGAATTACGGCCCGAATCCAAATGGGTGCTCGAGTTTGAACAGGGAGGCGGAGGAGAAGGCGATTAAAGAAAAGGGTTTTTACTTGCACCCTTCCCCGAGCACGACGCCGCGGGAATCGGAGCCCCGGTTGCTGCCTCTGTTCCCGACGACGTCGCCGAGAGCTGCTTCAGGTTCATCTTCTTGA

Protein Analysis

323

Amino Acids

35.03

Weight (kDa)

9.66

Isoelectric Point (pI)

64.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 137 - 163 1e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012106)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28280 AT1G28280
fragaria_vesca FvH4_3g09030 FvH4_3g09030 FvH4_3g09030 FvH4_3g09030
malus_domestica MD00G1116300.v1.1 MD10G1293000.v1.1
prunus_persica Prupe.4G048900_v2.0.a1
pyrus_communis pycom10g24500
rosa_chinensis RchiOBHm_Chr5g0008641
rosa_laevigata RLG00000031589
rosa_multiflora Rmu_sc0001665.1_g000029
rosa_rugosa Rorug04G0437600
rosa_samantha Rh5AG066200 Rh5BG063500 Rh5CG073700 Rh5DG062700
rosa_wichuraiana Rw5G006280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 938
AccB1I GGYRCC 1 cut(s) 237
AccBSI CCGCTC 1 cut(s) 144
AccII CGCG 4 cut(s) 28, 530, 682, 895
AccIII TCCGGA 2 cut(s) 627, 668
AclWI GGATC 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 936
AcyI GRCGYC 2 cut(s) 890, 935
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 4 cut(s) 220, 234, 247, 585
AgeI ACCGGT 1 cut(s) 771
AgsI TTSAA 4 cut(s) 451, 464, 592, 823
AjnI CCWGG 1 cut(s) 233
AluBI AGCT 8 cut(s) 294, 315, 347, 434, 595, 657, 665, 947
AluI AGCT 8 cut(s) 294, 315, 347, 434, 595, 657, 665, 947
Alw21I GWGCWC 3 cut(s) 349, 815, 887
Alw26I GTCTC 2 cut(s) 29, 106
AlwI GGATC 1 cut(s) 387
Ama87I CYCGRG 3 cut(s) 689, 814, 880
Aor13HI TCCGGA 2 cut(s) 627, 668
AoxI GGCC 1 cut(s) 793
ApeKI GCWGC 5 cut(s) 303, 312, 662, 916, 947
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AsiGI ACCGGT 1 cut(s) 771
AspLEI GCGC 2 cut(s) 28, 606
AspS9I GGNCC 3 cut(s) 582, 774, 794
AsuC2I CCSGG 4 cut(s) 580, 586, 778, 910
AsuHPI GGTGA 3 cut(s) 60, 222, 463
AsuII TTCGAA 1 cut(s) 506
AvaI CYCGRG 3 cut(s) 689, 814, 880
AvaII GGWCC 2 cut(s) 582, 774
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 2 cut(s) 349, 909
Bbv12I GWGCWC 3 cut(s) 349, 815, 887
BbvI GCAGC 5 cut(s) 315, 324, 674, 903, 934
BccI CCATC 3 cut(s) 223, 557, 656
BceAI ACGGC 3 cut(s) 355, 798, 808
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 1 cut(s) 235
BciVI GTATCC 1 cut(s) 423
BcnI CCSGG 4 cut(s) 580, 586, 778, 910
BcoDI GTCTC 2 cut(s) 29, 106
BfoI RGCGCY 1 cut(s) 607
BfuI GTATCC 1 cut(s) 423
BisI GCNGC 8 cut(s) 304, 313, 663, 680, 749, 893, 917, 948
BlpI GCTNAGC 1 cut(s) 492
BlsI GCNGC 8 cut(s) 305, 314, 664, 681, 750, 894, 918, 949
Bme1390I CCNGG 5 cut(s) 235, 580, 586, 778, 910
Bme18I GGWCC 2 cut(s) 582, 774
BmeT110I CYCGRG 3 cut(s) 689, 814, 880
BmgT120I GGNCC 3 cut(s) 582, 774, 794
BmiI GGNNCC 4 cut(s) 239, 478, 583, 906
BmrFI CCNGG 5 cut(s) 235, 580, 586, 778, 910
BmsI GCATC 1 cut(s) 17
Bpu1102I GCTNAGC 1 cut(s) 492
Bpu14I TTCGAA 1 cut(s) 506
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 4 cut(s) 580, 586, 778, 910
BsaHI GRCGYC 2 cut(s) 890, 935
BsaJI CCNNGG 3 cut(s) 233, 893, 908
BsaWI WCCGGW 4 cut(s) 247, 627, 668, 771
BsaXI ACNNNNNCTCC 6 cut(s) 349, 379, 428, 458, 896, 926
Bsc4I CCNNNNNNNGG 4 cut(s) 220, 234, 247, 585
Bse118I RCCGGY 2 cut(s) 473, 771
BseAI TCCGGA 2 cut(s) 627, 668
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 3 cut(s) 233, 893, 908
BseGI GGATG 2 cut(s) 515, 540
BseLI CCNNNNNNNGG 4 cut(s) 220, 234, 247, 585
BseMII CTCAG 2 cut(s) 196, 748
BseRI GAGGAG 4 cut(s) 7, 47, 433, 851
BseXI GCAGC 5 cut(s) 315, 324, 674, 903, 934
BseYI CCCAGC 1 cut(s) 369
Bsh1236I CGCG 4 cut(s) 28, 530, 682, 895
BshFI GGCC 1 cut(s) 795
BshNI GGYRCC 1 cut(s) 237
BshTI ACCGGT 1 cut(s) 771
BsiHKAI GWGCWC 3 cut(s) 349, 815, 887
BsiHKCI CYCGRG 3 cut(s) 689, 814, 880
BslFI GGGAC 1 cut(s) 793
BslI CCNNNNNNNGG 4 cut(s) 220, 234, 247, 585
BsmAI GTCTC 2 cut(s) 29, 106
BsmFI GGGAC 1 cut(s) 793
BsmI GAATGC 1 cut(s) 708
BsnI GGCC 1 cut(s) 795
BsoBI CYCGRG 3 cut(s) 689, 814, 880
Bsp119I TTCGAA 1 cut(s) 506
Bsp1286I GDGCHC 4 cut(s) 349, 815, 887, 909
Bsp13I TCCGGA 2 cut(s) 627, 668
Bsp143I GATC 1 cut(s) 392
Bsp1720I GCTNAGC 1 cut(s) 492
BspANI GGCC 1 cut(s) 795
BspCNI CTCAG 2 cut(s) 195, 747
BspEI TCCGGA 2 cut(s) 627, 668
BspFNI CGCG 4 cut(s) 28, 530, 682, 895
BspLI GGNNCC 4 cut(s) 239, 478, 583, 906
BspPI GGATC 1 cut(s) 387
BspT104I TTCGAA 1 cut(s) 506
BspT107I GGYRCC 1 cut(s) 237
BsrBI CCGCTC 1 cut(s) 144
BsrFI RCCGGY 2 cut(s) 473, 771
BssAI RCCGGY 2 cut(s) 473, 771
BssECI CCNNGG 3 cut(s) 233, 893, 908
BssMI GATC 1 cut(s) 392
BssNI GRCGYC 2 cut(s) 890, 935
Bst2UI CCWGG 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 2 cut(s) 135, 508
BstACI GRCGYC 2 cut(s) 890, 935
BstBI TTCGAA 1 cut(s) 506
BstC8I GCNNGC 2 cut(s) 498, 528
BstDEI CTNAG 3 cut(s) 182, 492, 734
BstDSI CCRYGG 1 cut(s) 893
BstENI CCTNNNNNAGG 1 cut(s) 232
BstF5I GGATG 2 cut(s) 515, 540
BstFNI CGCG 4 cut(s) 28, 530, 682, 895
BstH2I RGCGCY 1 cut(s) 607
BstHHI GCGC 2 cut(s) 28, 606
BstKTI GATC 1 cut(s) 395
BstMAI GTCTC 2 cut(s) 29, 106
BstMBI GATC 1 cut(s) 392
BstMWI GCNNNNNNNGC 7 cut(s) 300, 309, 312, 321, 497, 601, 913
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 5 cut(s) 233, 578, 584, 776, 908
BstUI CGCG 4 cut(s) 28, 530, 682, 895
BstV1I GCAGC 5 cut(s) 315, 324, 674, 903, 934
BstX2I RGATCY 1 cut(s) 392
BstYI RGATCY 1 cut(s) 392
BsuI GTATCC 1 cut(s) 423
BsuRI GGCC 1 cut(s) 795
BtgI CCRYGG 1 cut(s) 893
BtsCI GGATG 2 cut(s) 515, 540
Cac8I GCNNGC 2 cut(s) 498, 528
CfoI GCGC 2 cut(s) 28, 606
Cfr10I RCCGGY 2 cut(s) 473, 771
Cfr13I GGNCC 3 cut(s) 582, 774, 794
Cfr42I CCGCGG 1 cut(s) 896
CpoI CGGWCCG 1 cut(s) 774
CseI GACGC 1 cut(s) 898
Csp6I GTAC 1 cut(s) 4
CspAI ACCGGT 1 cut(s) 771
CspI CGGWCCG 1 cut(s) 774
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 3 cut(s) 182, 492, 734
DpnI GATC 1 cut(s) 394
DpnII GATC 1 cut(s) 392
Eam1104I CTCTTC 2 cut(s) 135, 508
EarI CTCTTC 2 cut(s) 135, 508
EciI GGCGGA 2 cut(s) 357, 848
Ecl136II GAGCTC 1 cut(s) 347
Eco24I GRGCYC 2 cut(s) 349, 909
Eco32I GATATC 1 cut(s) 756
Eco47I GGWCC 2 cut(s) 582, 774
Eco53kI GAGCTC 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 936
Eco88I CYCGRG 3 cut(s) 689, 814, 880
EcoICRI GAGCTC 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 233
EcoRV GATATC 1 cut(s) 756
EcoT38I GRGCYC 2 cut(s) 349, 909
FaiI YATR 1 cut(s) 111
FaqI GGGAC 1 cut(s) 793
FauI CCCGC 4 cut(s) 149, 531, 552, 888
Fnu4HI GCNGC 8 cut(s) 304, 313, 663, 680, 749, 893, 917, 948
FokI GGATG 2 cut(s) 502, 527
FriOI GRGCYC 2 cut(s) 349, 909
Fsp4HI GCNGC 8 cut(s) 304, 313, 663, 680, 749, 893, 917, 948
GlaI GCGC 2 cut(s) 27, 605
GluI GCNGC 8 cut(s) 304, 313, 663, 680, 749, 893, 917, 948
GsaI CCCAGC 1 cut(s) 373
HaeII RGCGCY 1 cut(s) 607
HaeIII GGCC 1 cut(s) 795
HgaI GACGC 1 cut(s) 898
HhaI GCGC 2 cut(s) 28, 606
Hin1I GRCGYC 2 cut(s) 890, 935
Hin6I GCGC 2 cut(s) 26, 604
HinP1I GCGC 2 cut(s) 26, 604
HinfI GANTC 5 cut(s) 37, 224, 672, 799, 899
HphI GGTGA 3 cut(s) 60, 222, 463
Hpy188I TCNGA 7 cut(s) 208, 397, 417, 520, 705, 760, 904
Hpy188III TCNNGA 7 cut(s) 619, 628, 669, 689, 713, 928, 966
Hpy99I CGWCG 4 cut(s) 892, 934, 937, 940
HpyAV CCTTC 5 cut(s) 273, 433, 457, 835, 885
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 935
HpyCH4V TGCA 1 cut(s) 871
HpyF10VI GCNNNNNNNGC 7 cut(s) 300, 309, 312, 321, 497, 601, 913
HpyF3I CTNAG 3 cut(s) 182, 492, 734
HpySE526I ACGT 1 cut(s) 935
Hsp92I GRCGYC 2 cut(s) 890, 935
HspAI GCGC 2 cut(s) 26, 604
Kpn2I TCCGGA 2 cut(s) 627, 668
KspI CCGCGG 1 cut(s) 896
Kzo9I GATC 1 cut(s) 392
LmnI GCTCC 8 cut(s) 149, 299, 320, 344, 352, 482, 662, 904
Lsp1109I GCAGC 5 cut(s) 315, 324, 674, 903, 934
LweI GCATC 1 cut(s) 17
MaeII ACGT 1 cut(s) 935
MaeIII GTNAC 1 cut(s) 742
MalI GATC 1 cut(s) 394
MbiI CCGCTC 1 cut(s) 144
MboI GATC 1 cut(s) 392
MboII GAAGA 3 cut(s) 122, 495, 954
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 4 cut(s) 349, 815, 887, 909
MluCI AATT 2 cut(s) 157, 787
MlyI GAGTC 1 cut(s) 31
MmeI TCCRAC 6 cut(s) 65, 321, 342, 440, 543, 783
MroI TCCGGA 2 cut(s) 627, 668
MseI TTAA 2 cut(s) 633, 849
MspA1I CMGCKG 2 cut(s) 751, 895
MspR9I CCNGG 5 cut(s) 235, 580, 586, 778, 910
Mva1269I GAATGC 1 cut(s) 708
MvaI CCWGG 1 cut(s) 235
MvnI CGCG 4 cut(s) 28, 530, 682, 895
MwoI GCNNNNNNNGC 7 cut(s) 300, 309, 312, 321, 497, 601, 913
NciI CCSGG 4 cut(s) 580, 586, 778, 910
NdeII GATC 1 cut(s) 392
NlaIV GGNNCC 4 cut(s) 239, 478, 583, 906
NmeAIII GCCGAG 1 cut(s) 966
NmuCI GTSAC 1 cut(s) 742
NspV TTCGAA 1 cut(s) 506
PaeR7I CTCGAG 1 cut(s) 814
PcsI WCGNNNNNNNCGW 1 cut(s) 938
PctI GAATGC 1 cut(s) 708
PfeI GAWTC 4 cut(s) 224, 672, 799, 899
PflFI GACNNNGTC 1 cut(s) 934
PfoI TCCNGGA 1 cut(s) 776
PinAI ACCGGT 1 cut(s) 771
PkrI GCNGC 8 cut(s) 305, 314, 664, 681, 750, 894, 918, 949
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
Psp124BI GAGCTC 1 cut(s) 349
Psp6I CCWGG 1 cut(s) 233
PspFI CCCAGC 1 cut(s) 369
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 4 cut(s) 239, 478, 583, 906
PspPI GGNCC 3 cut(s) 582, 774, 794
PspXI VCTCGAGB 1 cut(s) 814
PsuI RGATCY 1 cut(s) 392
PsyI GACNNNGTC 1 cut(s) 934
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
Rsr2I CGGWCCG 1 cut(s) 774
RsrII CGGWCCG 1 cut(s) 774
SacI GAGCTC 1 cut(s) 349
SacII CCGCGG 1 cut(s) 896
SaqAI TTAA 2 cut(s) 633, 849
SatI GCNGC 8 cut(s) 304, 313, 663, 680, 749, 893, 917, 948
Sau3AI GATC 1 cut(s) 392
Sau96I GGNCC 3 cut(s) 582, 774, 794
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 5 cut(s) 235, 580, 586, 778, 910
SduI GDGCHC 4 cut(s) 349, 815, 887, 909
SfaNI GCATC 1 cut(s) 17
Sfr274I CTCGAG 1 cut(s) 814
Sfr303I CCGCGG 1 cut(s) 896
SfuI TTCGAA 1 cut(s) 506
SgrBI CCGCGG 1 cut(s) 896
SinI GGWCC 2 cut(s) 582, 774
SlaI CTCGAG 1 cut(s) 814
SmlI CTYRAG 2 cut(s) 617, 814
SmoI CTYRAG 2 cut(s) 617, 814
Sse9I AATT 2 cut(s) 157, 787
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 5 cut(s) 233, 578, 584, 776, 908
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 1 cut(s) 938
TaqI TCGA 3 cut(s) 506, 714, 815
TasI AATT 2 cut(s) 157, 787
TauI GCSGC 3 cut(s) 682, 751, 895
TfiI GAWTC 4 cut(s) 224, 672, 799, 899
Tru1I TTAA 2 cut(s) 633, 849
Tru9I TTAA 2 cut(s) 633, 849
TseFI GTSAC 1 cut(s) 742
TseI GCWGC 5 cut(s) 303, 312, 662, 916, 947
Tsp45I GTSAC 1 cut(s) 742
TspDTI ATGAA 1 cut(s) 948
Tth111I GACNNNGTC 1 cut(s) 934
VpaK11BI GGWCC 2 cut(s) 582, 774
XagI CCTNNNNNAGG 1 cut(s) 232
XhoI CTCGAG 1 cut(s) 814
ZraI GACGTC 1 cut(s) 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.