Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0006919

Physical Location & Seq

Genomic Coordinates Forward (+)

26614910 .. 26616484

Gene Structure 1575 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGGACAGGAGAAATGTGTTAATTGGTCTCGGAGGAGCAGGTCTTGGTCTTGGTCTTGACACCAATCCATTGGCCTCTGGTGCACCAATTCCCCCTCCGGACTTTAGCACGTGCGGCAAGACTACGGACGAAGTAACAGGACTTGATTGTTGTCCGCCAACAAGTGAAGTCAAAACTCCCACTCCCTTCAAGCCAGTAGCGCCCCCAAAAAGCTCAGTCCGCACTCGGCTAGCAGCTCAGAATGCTGTCAAGGATCCTGACTACATAAAGAAGTACAGAAGAGCCACTGAGCTCATGAAGGGCCTTGACGTTGACGACCCGCGTAATTTCTGGAACCAAGCTAATGTTCATTGCAGCTATTGCGATGGTGCGTTTAATCAAAACTTAGCTGGGTACAAATGTCTGGAGATACAAGTTCATAATTCGTGGCTCTTCTTTCCCTTCCACCGCTGGTTCCTCTTCTTCTACGAGAGAATCCTCGGTAGCCTTATTGACGACCCGACTTTCGCTCTGCCATTTTGGAATTGGGACAGTCCTAATGGCATGTACTTGCCTGAATTGTTTGAGCTCGACAACGGCGCCTCCCCTCTGTACGACCACTACCGCAACACCAAGCACCGGCAGAAGGATTTCTTGCTGGACCTCAACTACAGTGGTACAGACGTCCACCAAGACCCAAAAACGCTAATAGATAATAACCTCAAAACCATGAACCGGCAGATGAATACCAAGGACCAGTGCCTATTCTTTGGGCAACCTTATAGGGCTGGCGACTGTCCAAACCCCGGTGGAGGAAACATTGAGCTTATACCCCACAATACGGTCCACAACTGGACCGGTGACCCCAAGTGGCTTGCGGAGGACATGGGAAAGTTCTACTCTTCCGCTCGAGATCCAATCTTTTTTGCACACCACTCCAACGTGGACCGCATGTGGCACCTGTGGCTCGAGAAAATAGGAGGCAAGAATTTGGAACAAGATGACTGGCTTAACGCCGAGTTTTATTTCTATGACGAGAAGAAGGAACTGGTGAGTGTTACCGTCCAGGACTCGATTGACATTGGAAAACTCGGCTATGCTTACGAAAATGTTGACATTCCGTGGCTCCAGACCAAGCCAACTGCACGTAAACCAGCGAATAAGGCGAAACCTGATGGTTTAGGACTCCAAGATCAGTCCTCTGCAGCTAAAGGTGGATTCTCTTTGGACTCCAAGATCAGCATTGCGGTGCCGAGACCAAAGCAGGGCGTGGAGAAGGCGGAACGAGAGGAGATATTGATCTTGCGAGACATTAAATTTCCGAGCGACCAGGGTTTGAAGTTCGATGTATACGTGAATGATGATGCCGACACGCCAAGTCAGCCCAGCCAGAGTGAGTTTGCGGGGAGCTTTGTGCATGTCCCGCACAAGCACGGCATGACTTTTAAGACCAGCCTGTATTTGGGAATCACGACGTTGTTGAAGGACGTGGGAGCCGATAAGGCCGCTAGTGTGGTGGTCACTTTGGTGCCAAGGTACCGGAATGGGCCGGTCAGCATTGGGGGCATCAGCATCGAGCAATCTTCTTGCGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

525

Amino Acids

58.76

Weight (kDa)

5.81

Isoelectric Point (pI)

42.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	108 - 317	4.3e-35	Common central domain of tyrosinase
PPO1_DWL	PF12142	324 - 374	5.8e-22	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	401 - 519	1.5e-36	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04680
malus_domestica	MD05G1319800.v1.1 MD05G1320100.v1.1 MD05G1320200.v1.1 MD05G1320500.v1.1 MD05G1320800.v1.1 MD10G1298300.v1.1
prunus_persica	Prupe.4G041500_v2.0.a1 Prupe.4G041700_v2.0.a1
pyrus_communis	pycom05g29530 pycom05g29560 pycom10g24890 pycom10g24910 pycom10g25140
rosa_chinensis	RchiOBHm_Chr5g0007621
rosa_laevigata	RLG00000031479
rosa_multiflora	Rmu_co8439317.1_g000001 Rmu_sc0011744.1_g000006 Rmu_sc0021005.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068730
rosa_rugosa	Rorug04G0429400
rosa_samantha	Rh5AG057500 Rh5BG054400 Rh5CG064400 Rh5DG053600
rosa_wichuraiana	Rw5G006180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	666
Acc36I	ACCTGC	1 cut(s)	29
Acc65I	GGTACC	1 cut(s)	1515
AccB1I	GGYRCC	5 cut(s)	578, 936, 1228, 1507, 1515
AccBSI	CCGCTC	1 cut(s)	887
AccI	GTMKAC	1 cut(s)	1329
AccII	CGCG	1 cut(s)	322
AccIII	TCCGGA	1 cut(s)	97
AclWI	GGATC	3 cut(s)	248, 261, 887
AcsI	RAATTY	2 cut(s)	967, 1295
AcvI	CACGTG	1 cut(s)	111
AcyI	GRCGYC	2 cut(s)	579, 663
AfaI	GTAC	6 cut(s)	275, 395, 548, 593, 658, 1517
AfiI	CCNNNNNNNGG	8 cut(s)	618, 625, 714, 785, 791, 820, 1244, 1441
AgeI	ACCGGT	1 cut(s)	836
AgsI	TTSAA	3 cut(s)	190, 1318, 1462
AjiI	CACGTC	1 cut(s)	1468
AjnI	CCWGG	2 cut(s)	1044, 1308
AjuI	GAANNNNNNNTTGG	2 cut(s)	330, 362
AleI	CACNNNNGTG	1 cut(s)	1505
Alw21I	GWGCWC	3 cut(s)	85, 294, 570
Alw26I	GTCTC	3 cut(s)	32, 1228, 1281
Alw44I	GTGCAC	1 cut(s)	81
AlwI	GGATC	3 cut(s)	248, 261, 887
Ama87I	CYCGRG	2 cut(s)	888, 947
Aor13HI	TCCGGA	1 cut(s)	97
AoxI	GGCC	4 cut(s)	72, 301, 1482, 1526
ApaLI	GTGCAC	1 cut(s)	81
ApeKI	GCWGC	3 cut(s)	233, 354, 1184
ApoI	RAATTY	2 cut(s)	967, 1295
ArsI	GACNNNNNNTTYG	2 cut(s)	488, 520
AsiGI	ACCGGT	1 cut(s)	836
Asp700I	GAANNNNTTC	1 cut(s)	629
Asp718I	GGTACC	1 cut(s)	1515
AspLEI	GCGC	2 cut(s)	202, 581
AspS9I	GGNCC	7 cut(s)	301, 640, 733, 823, 834, 925, 1526
AsuC2I	CCSGG	1 cut(s)	786
AsuHPI	GGTGA	2 cut(s)	851, 1042
AsuNHI	GCTAGC	1 cut(s)	229
AvaI	CYCGRG	2 cut(s)	888, 947
AvaII	GGWCC	5 cut(s)	640, 733, 823, 834, 925
BaeGI	GKGCMC	1 cut(s)	85
BaeI	ACNNNNGTAYC	4 cut(s)	385, 418, 648, 681
BamHI	GGATCC	1 cut(s)	253
BanI	GGYRCC	5 cut(s)	578, 936, 1228, 1507, 1515
BanII	GRGCYC	2 cut(s)	294, 570
BbrPI	CACGTG	1 cut(s)	111
Bbv12I	GWGCWC	3 cut(s)	85, 294, 570
BbvI	GCAGC	3 cut(s)	245, 366, 1196
BccI	CCATC	2 cut(s)	359, 1148
BceAI	ACGGC	2 cut(s)	592, 1429
BcgI	CGANNNNNNTGC	2 cut(s)	1534, 1568
BciT130I	CCWGG	2 cut(s)	1046, 1310
BcnI	CCSGG	1 cut(s)	786
BcoDI	GTCTC	3 cut(s)	32, 1228, 1281
BfaI	CTAG	2 cut(s)	230, 1488
BfmI	CTRYAG	2 cut(s)	649, 1182
BfoI	RGCGCY	2 cut(s)	203, 582
BfuAI	ACCTGC	1 cut(s)	29
BglI	GCCNNNNNGGC	1 cut(s)	1481
BisI	GCNGC	5 cut(s)	115, 234, 355, 1185, 1485
BlsI	GCNGC	5 cut(s)	116, 235, 356, 1186, 1486
Bme1390I	CCNGG	3 cut(s)	786, 1046, 1310
Bme18I	GGWCC	5 cut(s)	640, 733, 823, 834, 925
BmeT110I	CYCGRG	2 cut(s)	888, 947
BmgBI	CACGTC	1 cut(s)	1468
BmgT120I	GGNCC	7 cut(s)	301, 640, 733, 823, 834, 925, 1526
BmrFI	CCNGG	3 cut(s)	786, 1046, 1310
BmsI	GCATC	3 cut(s)	1333, 1554, 1560
BmtI	GCTAGC	1 cut(s)	233
BpmI	CTGGAG	2 cut(s)	425, 1091
BpuMI	CCSGG	1 cut(s)	786
BsaAI	YACGTR	3 cut(s)	111, 1127, 1333
BsaBI	GATNNNNATC	1 cut(s)	1277
BsaHI	GRCGYC	2 cut(s)	579, 663
BsaI	GGTCTC	2 cut(s)	32, 1228
BsaJI	CCNNGG	6 cut(s)	478, 729, 784, 1100, 1309, 1511
BsaWI	WCCGGW	3 cut(s)	97, 836, 1518
BsaXI	ACNNNNNCTCC	4 cut(s)	166, 196, 566, 596
Bsc4I	CCNNNNNNNGG	8 cut(s)	618, 625, 714, 785, 791, 820, 1244, 1441
Bse118I	RCCGGY	4 cut(s)	618, 714, 836, 1528
Bse1I	ACTGG	5 cut(s)	194, 736, 836, 989, 1032
Bse3DI	GCAATG	2 cut(s)	349, 1221
Bse8I	GATNNNNATC	1 cut(s)	1277
BseAI	TCCGGA	1 cut(s)	97
BseBI	CCWGG	2 cut(s)	1046, 1310
BseDI	CCNNGG	6 cut(s)	478, 729, 784, 1100, 1309, 1511
BseJI	GATNNNNATC	1 cut(s)	1277
BseLI	CCNNNNNNNGG	8 cut(s)	618, 625, 714, 785, 791, 820, 1244, 1441
BseMI	GCAATG	2 cut(s)	349, 1221
BseMII	CTCAG	3 cut(s)	228, 251, 279
BseNI	ACTGG	5 cut(s)	194, 736, 836, 989, 1032
BseRI	GAGGAG	2 cut(s)	48, 1283
BseSI	GKGCMC	1 cut(s)	85
BseXI	GCAGC	3 cut(s)	245, 366, 1196
BseYI	CCCAGC	2 cut(s)	389, 1364
BsgI	GTGCAG	1 cut(s)	1107
Bsh1236I	CGCG	1 cut(s)	322
BshFI	GGCC	4 cut(s)	74, 303, 1484, 1528
BshNI	GGYRCC	5 cut(s)	578, 936, 1228, 1507, 1515
BshTI	ACCGGT	1 cut(s)	836
BsiHKAI	GWGCWC	3 cut(s)	85, 294, 570
BsiHKCI	CYCGRG	2 cut(s)	888, 947
BsiSI	CCGG	7 cut(s)	98, 619, 715, 786, 837, 1519, 1529
BslFI	GGGAC	2 cut(s)	542, 1385
BslI	CCNNNNNNNGG	8 cut(s)	618, 625, 714, 785, 791, 820, 1244, 1441
BsmAI	GTCTC	3 cut(s)	32, 1228, 1281
BsmFI	GGGAC	2 cut(s)	542, 1385
BsmI	GAATGC	1 cut(s)	247
BsnI	GGCC	4 cut(s)	74, 303, 1484, 1528
Bso31I	GGTCTC	2 cut(s)	32, 1228
BsoBI	CYCGRG	2 cut(s)	888, 947
Bsp1286I	GDGCHC	3 cut(s)	85, 294, 570
Bsp13I	TCCGGA	1 cut(s)	97
Bsp143I	GATC	5 cut(s)	253, 892, 1171, 1215, 1278
BspANI	GGCC	4 cut(s)	74, 303, 1484, 1528
BspCNI	CTCAG	3 cut(s)	227, 250, 280
BspEI	TCCGGA	1 cut(s)	97
BspFNI	CGCG	1 cut(s)	322
BspHI	TCATGA	1 cut(s)	294
BspMAI	CTGCAG	1 cut(s)	1186
BspMI	ACCTGC	1 cut(s)	29
BspOI	GCTAGC	1 cut(s)	233
BspPI	GGATC	3 cut(s)	248, 261, 887
BspQI	GCTCTTC	2 cut(s)	274, 437
BspT107I	GGYRCC	5 cut(s)	578, 936, 1228, 1507, 1515
BspTNI	GGTCTC	2 cut(s)	32, 1228
BsrBI	CCGCTC	1 cut(s)	887
BsrDI	GCAATG	2 cut(s)	349, 1221
BsrFI	RCCGGY	4 cut(s)	618, 714, 836, 1528
BsrI	ACTGG	5 cut(s)	194, 736, 836, 989, 1032
BssAI	RCCGGY	4 cut(s)	618, 714, 836, 1528
BssECI	CCNNGG	6 cut(s)	478, 729, 784, 1100, 1309, 1511
BssMI	GATC	5 cut(s)	253, 892, 1171, 1215, 1278
BssNAI	GTATAC	1 cut(s)	1330
BssNI	GRCGYC	2 cut(s)	579, 663
BssT1I	CCWWGG	2 cut(s)	729, 1511
Bst1107I	GTATAC	1 cut(s)	1330
Bst2UI	CCWGG	2 cut(s)	1046, 1310
Bst4CI	ACNGT	5 cut(s)	533, 653, 776, 823, 1042
Bst6I	CTCTTC	4 cut(s)	274, 437, 464, 886
BstACI	GRCGYC	2 cut(s)	579, 663
BstAPI	GCANNNNNTGC	1 cut(s)	360
BstBAI	YACGTR	3 cut(s)	111, 1127, 1333
BstC8I	GCNNGC	3 cut(s)	231, 769, 855
BstDEI	CTNAG	4 cut(s)	214, 237, 288, 385
BstDSI	CCRYGG	1 cut(s)	1100
BstEII	GGTNACC	1 cut(s)	839
BstFNI	CGCG	1 cut(s)	322
BstH2I	RGCGCY	2 cut(s)	203, 582
BstHHI	GCGC	2 cut(s)	202, 581
BstKTI	GATC	5 cut(s)	256, 895, 1174, 1218, 1281
BstMAI	GTCTC	3 cut(s)	32, 1228, 1281
BstMBI	GATC	5 cut(s)	253, 892, 1171, 1215, 1278
BstNI	CCWGG	2 cut(s)	1046, 1310
BstNSI	RCATGY	3 cut(s)	547, 934, 1400
BstPI	GGTNACC	1 cut(s)	839
BstSCI	CCNGG	3 cut(s)	784, 1044, 1308
BstSFI	CTRYAG	2 cut(s)	649, 1182
BstSLI	GKGCMC	1 cut(s)	85
BstUI	CGCG	1 cut(s)	322
BstV1I	GCAGC	3 cut(s)	245, 366, 1196
BstX2I	RGATCY	2 cut(s)	253, 892
BstXI	CCANNNNNNTGG	1 cut(s)	70
BstYI	RGATCY	2 cut(s)	253, 892
BstZ17I	GTATAC	1 cut(s)	1330
BsuRI	GGCC	4 cut(s)	74, 303, 1484, 1528
BtgI	CCRYGG	1 cut(s)	1100
BtgZI	GCGATG	1 cut(s)	378
BtrI	CACGTC	1 cut(s)	1468
BtsIMutI	CAGTG	3 cut(s)	285, 658, 743
BveI	ACCTGC	1 cut(s)	29
Cac8I	GCNNGC	3 cut(s)	231, 769, 855
CciI	TCATGA	1 cut(s)	294
CfoI	GCGC	2 cut(s)	202, 581
Cfr10I	RCCGGY	4 cut(s)	618, 714, 836, 1528
Cfr13I	GGNCC	7 cut(s)	301, 640, 733, 823, 834, 925, 1526
Csp6I	GTAC	6 cut(s)	274, 394, 547, 592, 657, 1516
CspAI	ACCGGT	1 cut(s)	836
CspCI	CAANNNNNGTGG	2 cut(s)	634, 669
CviAII	CATG	7 cut(s)	295, 544, 709, 865, 931, 1397, 1417
CviQI	GTAC	6 cut(s)	274, 394, 547, 592, 657, 1516
DdeI	CTNAG	4 cut(s)	214, 237, 288, 385
DinI	GGCGCC	1 cut(s)	580
DpnI	GATC	5 cut(s)	255, 894, 1173, 1217, 1280
DpnII	GATC	5 cut(s)	253, 892, 1171, 1215, 1278
Eam1104I	CTCTTC	4 cut(s)	274, 437, 464, 886
EarI	CTCTTC	4 cut(s)	274, 437, 464, 886
EciI	GGCGGA	2 cut(s)	144, 1274
Ecl136II	GAGCTC	2 cut(s)	292, 568
Eco130I	CCWWGG	2 cut(s)	729, 1511
Eco24I	GRGCYC	2 cut(s)	294, 570
Eco31I	GGTCTC	2 cut(s)	32, 1228
Eco47I	GGWCC	5 cut(s)	640, 733, 823, 834, 925
Eco53kI	GAGCTC	2 cut(s)	292, 568
Eco72I	CACGTG	1 cut(s)	111
Eco88I	CYCGRG	2 cut(s)	888, 947
Eco91I	GGTNACC	1 cut(s)	839
EcoICRI	GAGCTC	2 cut(s)	292, 568
EcoO109I	RGGNCCY	1 cut(s)	301
EcoO65I	GGTNACC	1 cut(s)	839
EcoRII	CCWGG	2 cut(s)	1044, 1308
EcoT14I	CCWWGG	2 cut(s)	729, 1511
EcoT38I	GRGCYC	2 cut(s)	294, 570
EgeI	GGCGCC	1 cut(s)	580
EheI	GGCGCC	1 cut(s)	580
ErhI	CCWWGG	2 cut(s)	729, 1511
FaeI	CATG	7 cut(s)	298, 547, 712, 868, 934, 1400, 1420
FalI	AAGNNNNNCTT	2 cut(s)	617, 649
FaqI	GGGAC	2 cut(s)	542, 1385
FatI	CATG	7 cut(s)	294, 543, 708, 864, 930, 1396, 1416
FauI	CCCGC	3 cut(s)	327, 1375, 1410
FblI	GTMKAC	1 cut(s)	1329
Fnu4HI	GCNGC	5 cut(s)	115, 234, 355, 1185, 1485
FriOI	GRGCYC	2 cut(s)	294, 570
Fsp4HI	GCNGC	5 cut(s)	115, 234, 355, 1185, 1485
FspBI	CTAG	2 cut(s)	230, 1488
GlaI	GCGC	2 cut(s)	201, 580
GluI	GCNGC	5 cut(s)	115, 234, 355, 1185, 1485
GsaI	CCCAGC	2 cut(s)	393, 1368
GsuI	CTGGAG	2 cut(s)	425, 1091
HaeII	RGCGCY	2 cut(s)	203, 582
HaeIII	GGCC	4 cut(s)	74, 303, 1484, 1528
HapII	CCGG	7 cut(s)	98, 619, 715, 786, 837, 1519, 1529
HhaI	GCGC	2 cut(s)	202, 581
Hin1I	GRCGYC	2 cut(s)	579, 663
Hin1II	CATG	7 cut(s)	298, 547, 712, 868, 934, 1400, 1420
Hin6I	GCGC	2 cut(s)	200, 579
HinP1I	GCGC	2 cut(s)	200, 579
HincII	GTYRAC	2 cut(s)	313, 1093
HindII	GTYRAC	2 cut(s)	313, 1093
HinfI	GANTC	6 cut(s)	474, 1049, 1164, 1197, 1208, 1446
HpaII	CCGG	7 cut(s)	98, 619, 715, 786, 837, 1519, 1529
HphI	GGTGA	2 cut(s)	851, 1042
Hpy166II	GTNNAC	8 cut(s)	83, 313, 667, 826, 925, 1093, 1130, 1330
Hpy188I	TCNGA	3 cut(s)	32, 240, 1302
Hpy8I	GTNNAC	8 cut(s)	83, 313, 667, 826, 925, 1093, 1130, 1330
Hpy99I	CGWCG	1 cut(s)	1456
HpyAV	CCTTC	7 cut(s)	196, 292, 451, 619, 1015, 1249, 1456
HpyCH4III	ACNGT	5 cut(s)	533, 653, 776, 823, 1042
HpyCH4IV	ACGT	8 cut(s)	110, 309, 663, 921, 1126, 1332, 1454, 1467
HpyCH4V	TGCA	6 cut(s)	83, 354, 908, 1124, 1184, 1396
HpyF3I	CTNAG	4 cut(s)	214, 237, 288, 385
HpySE526I	ACGT	8 cut(s)	110, 309, 663, 921, 1126, 1332, 1454, 1467
Hsp92I	GRCGYC	2 cut(s)	579, 663
Hsp92II	CATG	7 cut(s)	298, 547, 712, 868, 934, 1400, 1420
HspAI	GCGC	2 cut(s)	200, 579
KasI	GGCGCC	1 cut(s)	578
Kpn2I	TCCGGA	1 cut(s)	97
KpnI	GGTACC	1 cut(s)	1519
Kzo9I	GATC	5 cut(s)	253, 892, 1171, 1215, 1278
LguI	GCTCTTC	2 cut(s)	274, 437
LmnI	GCTCC	4 cut(s)	35, 1110, 1386, 1472
Lsp1109I	GCAGC	3 cut(s)	245, 366, 1196
LweI	GCATC	3 cut(s)	1333, 1554, 1560
MaeI	CTAG	2 cut(s)	230, 1488
MaeII	ACGT	8 cut(s)	110, 309, 663, 921, 1126, 1332, 1454, 1467
MaeIII	GTNAC	4 cut(s)	133, 839, 1036, 1498
MalI	GATC	5 cut(s)	255, 894, 1173, 1217, 1280
MbiI	CCGCTC	1 cut(s)	887
MboI	GATC	5 cut(s)	253, 892, 1171, 1215, 1278
MboII	GAAGA	7 cut(s)	291, 424, 451, 454, 873, 1030, 1554
MflI	RGATCY	2 cut(s)	253, 892
MhlI	GDGCHC	3 cut(s)	85, 294, 570
MluCI	AATT	8 cut(s)	21, 87, 325, 421, 523, 557, 967, 1295
Mly113I	GGCGCC	1 cut(s)	579
MlyI	GAGTC	3 cut(s)	1043, 1158, 1202
MmeI	TCCRAC	1 cut(s)	942
MroI	TCCGGA	1 cut(s)	97
MroXI	GAANNNNTTC	1 cut(s)	629
MseI	TTAA	5 cut(s)	20, 375, 990, 1293, 1425
MslI	CAYNNNNRTG	2 cut(s)	1226, 1505
MspA1I	CMGCKG	1 cut(s)	450
MspI	CCGG	7 cut(s)	98, 619, 715, 786, 837, 1519, 1529
MspR9I	CCNGG	3 cut(s)	786, 1046, 1310
Mva1269I	GAATGC	1 cut(s)	247
MvaI	CCWGG	2 cut(s)	1046, 1310
MvnI	CGCG	1 cut(s)	322
NarI	GGCGCC	1 cut(s)	579
NciI	CCSGG	1 cut(s)	786
NdeII	GATC	5 cut(s)	253, 892, 1171, 1215, 1278
NheI	GCTAGC	1 cut(s)	229
NlaIII	CATG	7 cut(s)	298, 547, 712, 868, 934, 1400, 1420
NmeAIII	GCCGAG	4 cut(s)	205, 1021, 1050, 1257
NmuCI	GTSAC	2 cut(s)	839, 1498
NspI	RCATGY	3 cut(s)	547, 934, 1400
OliI	CACNNNNGTG	1 cut(s)	1505
PaeR7I	CTCGAG	2 cut(s)	888, 947
PagI	TCATGA	1 cut(s)	294
PciSI	GCTCTTC	2 cut(s)	274, 437
PcsI	WCGNNNNNNNCGW	2 cut(s)	1329, 1473
PctI	GAATGC	1 cut(s)	247
PdmI	GAANNNNTTC	1 cut(s)	629
PfeI	GAWTC	3 cut(s)	474, 1197, 1446
PfoI	TCCNGGA	1 cut(s)	1044
PinAI	ACCGGT	1 cut(s)	836
PkrI	GCNGC	5 cut(s)	116, 235, 356, 1186, 1486
PleI	GAGTC	3 cut(s)	1043, 1158, 1202
PluTI	GGCGCC	1 cut(s)	582
PmaCI	CACGTG	1 cut(s)	111
PmlI	CACGTG	1 cut(s)	111
PpsI	GAGTC	3 cut(s)	1043, 1158, 1202
Ppu21I	YACGTR	3 cut(s)	111, 1127, 1333
Psp124BI	GAGCTC	2 cut(s)	294, 570
Psp6I	CCWGG	2 cut(s)	1044, 1308
PspCI	CACGTG	1 cut(s)	111
PspEI	GGTNACC	1 cut(s)	839
PspFI	CCCAGC	2 cut(s)	389, 1364
PspGI	CCWGG	2 cut(s)	1044, 1308
PspPI	GGNCC	7 cut(s)	301, 640, 733, 823, 834, 925, 1526
PstI	CTGCAG	1 cut(s)	1186
PsuI	RGATCY	2 cut(s)	253, 892
RsaI	GTAC	6 cut(s)	275, 395, 548, 593, 658, 1517
RsaNI	GTAC	6 cut(s)	274, 394, 547, 592, 657, 1516
RseI	CAYNNNNRTG	2 cut(s)	1226, 1505
SacI	GAGCTC	2 cut(s)	294, 570
SapI	GCTCTTC	2 cut(s)	274, 437
SaqAI	TTAA	5 cut(s)	20, 375, 990, 1293, 1425
SatI	GCNGC	5 cut(s)	115, 234, 355, 1185, 1485
Sau3AI	GATC	5 cut(s)	253, 892, 1171, 1215, 1278
Sau96I	GGNCC	7 cut(s)	301, 640, 733, 823, 834, 925, 1526
SchI	GAGTC	3 cut(s)	1043, 1158, 1202
ScrFI	CCNGG	3 cut(s)	786, 1046, 1310
SduI	GDGCHC	3 cut(s)	85, 294, 570
SfaNI	GCATC	3 cut(s)	1333, 1554, 1560
SfcI	CTRYAG	2 cut(s)	649, 1182
SfoI	GGCGCC	1 cut(s)	580
Sfr274I	CTCGAG	2 cut(s)	888, 947
SinI	GGWCC	5 cut(s)	640, 733, 823, 834, 925
SlaI	CTCGAG	2 cut(s)	888, 947
SmiMI	CAYNNNNRTG	2 cut(s)	1226, 1505
SmlI	CTYRAG	2 cut(s)	888, 947
SmoI	CTYRAG	2 cut(s)	888, 947
Sse9I	AATT	8 cut(s)	21, 87, 325, 421, 523, 557, 967, 1295
SspDI	GGCGCC	1 cut(s)	578
SspMI	CTAG	2 cut(s)	230, 1488
SstI	GAGCTC	2 cut(s)	294, 570
StyD4I	CCNGG	3 cut(s)	784, 1044, 1308
StyI	CCWWGG	2 cut(s)	729, 1511
TaaI	ACNGT	5 cut(s)	533, 653, 776, 823, 1042
TaiI	ACGT	8 cut(s)	113, 312, 666, 924, 1129, 1335, 1457, 1470
TaqI	TCGA	6 cut(s)	570, 889, 948, 1052, 1323, 1554
TasI	AATT	8 cut(s)	21, 87, 325, 421, 523, 557, 967, 1295
TatI	WGTACW	2 cut(s)	273, 546
TauI	GCSGC	2 cut(s)	117, 1487
TfiI	GAWTC	3 cut(s)	474, 1197, 1446
Tru1I	TTAA	5 cut(s)	20, 375, 990, 1293, 1425
Tru9I	TTAA	5 cut(s)	20, 375, 990, 1293, 1425
TscAI	CASTG	3 cut(s)	292, 658, 743
TseFI	GTSAC	2 cut(s)	839, 1498
TseI	GCWGC	3 cut(s)	233, 354, 1184
Tsp45I	GTSAC	2 cut(s)	839, 1498
TspDTI	ATGAA	5 cut(s)	311, 338, 407, 725, 737
TspGWI	ACGGA	2 cut(s)	140, 1089
TspRI	CASTG	3 cut(s)	292, 658, 743
VneI	GTGCAC	1 cut(s)	81
VpaK11BI	GGWCC	5 cut(s)	640, 733, 823, 834, 925
XapI	RAATTY	2 cut(s)	967, 1295
XceI	RCATGY	3 cut(s)	547, 934, 1400
XcmI	CCANNNNNNNNNTGG	2 cut(s)	522, 1438
XhoI	CTCGAG	2 cut(s)	888, 947
XmiI	GTMKAC	1 cut(s)	1329
XmnI	GAANNNNTTC	1 cut(s)	629
XspI	CTAG	2 cut(s)	230, 1488
ZraI	GACGTC	1 cut(s)	664

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.