Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0006919

Physical Location & Seq

Genomic Coordinates Forward (+)

26636289 .. 26638148

Gene Structure 1860 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGCATGTTCATTGTTGCAAGCAACCTACCATACCATTTATGCAAGCCATGAACACTCATCTGCTTCTTTCACCCCCTACTACAGTTATTGACCGTCGACCTAATGATTTGTTCCCAAAGAAGCTGTCCTCCCAAAGTAGCTGTGTAAATCGGAAGGGAAGGCGGAGCTGCTCTGTAGGCTACAAAGGAGCAAATGGTGCTCACAATATTAACCCTACCTGCCGTATCAAGCCGGAGGCCGACGAGGATAGGAGAAAAAAAATGGACAGGAGAAATGTGTTAATTGGTCTTGGAGGAGGTCTTGGTCTTGGAGGAGCAGGTCTTGGTCTTGGTCGTGGTCTTGACACCAATCCATTGCCCTCTGGTGCACCAATTCCCCCTCCGGACTTTACCATGTGCGGCAAGACTACGGGCGAAGTAACAGGACTTGATTGTTGTCCGCCAACAAGTGAAGTCAAAACTCCCACTCCCTTCAAGCCAGTAGCGCCCCCAAAAAGCTCAGTCCGCACTCGGCTAGCAGCTCAGAATGCTGTCAAGGATCCTGACTACATAAAGAAGTACAGAAGAGCCACTGAGCTCATGAAGGGCCTTGACGTTGACGACCCGCGTAATTTCTGGAACCAAGCTAATGTTCATTGCAGCTATTGCGATGGTGCGTTTAATCAAAACTTAGCTGGGTACAAATGTCTGGAGATACAAGTTCATAGTTCGTGGCTCTTCTTTCCCTTCCACCGCTGGTACCTCTTCTTCTACGAGAGAATCCTCGGTAGCCTTATTGACGACCCGACTTTCGCTCTGCCATTTTGGAATTGGGACAGTCCAGATGGCATGTACTTGCCTGAATTGTTTGAGCTCCACAACGGCGCCTCCCCTCTGTACGACCACTACCGCAACACCAAGCACCGGCAGAAGGATTTCTTGCTGGACCTCAACTACAGTGGTACAGACGTCGCCCAAGACCCAAAAACGCTAATAGATAATAACCTCAAAACCATGAACCGGCAGATGAATACCAAGGACCGGTGCCTATTCTTTGGGCAACCTTATAGGGCTGGCGACTGTCCAAACCCCGGTGCAGGAAACATTGAGCTTGTACCCCACAATACGGTCCACAACTGGACCGGTGACCCCAAGTGGCGTGCGGAGGACATGGGAAACTTTTACTCTGCCGGTCGAGATCCAATCTTTTTTTCACACCACTCCAACGTGGACCGCATGTGGCACCTATGGATCGAGAAAATAGGAGGCAAGAATTTGGAACAAGATGACTGGCTTAACGCCGAGTTTTTATTCTATAACGAGAAGAAGGAACTGGTGAGTGTTAACGTCCAGGACTCTCTCGACATTGGAAAACTCGGCTATGCTTACGAAAATGTTGACATTCCGTGGCTCCAGACCAAGCCAACTGCACGTAAACCAGCGAATAAGGCGAAACCTGATGGTTTAGGACTCCAAGATCAGTCCTCTGCAGCTAAAGGTGGATTCTCTTTGGACTCCAAGATCAGCATTGCGGTGCCGAGACCAAAGCAGGGCGTGGAGAAGGCGGAACGAGAGGAGATATTGATCTTGCGAGACATTAAATTTCCGAGCGACCAGGGTTTGAAGTTCGATGTATACGTGAATGATGATGCCGACACGCCTAGTCAGCCCAGCCAGAGTGAATTTGCGGGGAGCTTTGTGCATGTCCCGCACAAGCACAGCATGACTTTTAAGACCAACCTGTATTTGGGAATCACGACGTTGTTGAAGGACGTGGGAGCCGATAAGGCCGCTAGTGTTGTGGTCACTTTGGTGCCAAGGTACCGGAATGGGCCGGTCAGCATTGGGGGCATCAGCATCGAGCAATCTTCTTGTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009536 GO:0009543 GO:0009579 GO:0031976 GO:0031977 GO:0031978 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00350 ko00950 ko01100 ko01110 map00350 map00950 map01100 map01110

Pfam Domains

Protein Families

PPO1_DWL PPO1_KFDV Tyrosinase

Protein Analysis

620

Amino Acids

69.25

Weight (kDa)

8.25

Isoelectric Point (pI)

46.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyrosinase	PF00264	203 - 412	2e-34	Common central domain of tyrosinase
PPO1_DWL	PF12142	419 - 469	1.9e-21	Polyphenol oxidase middle domain
PPO1_KFDV	PF12143	496 - 614	1.5e-36	Chloroplastic Polyphenol Oxidase C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006919)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04680
malus_domestica	MD05G1319800.v1.1 MD05G1320100.v1.1 MD05G1320200.v1.1 MD05G1320500.v1.1 MD05G1320800.v1.1 MD10G1298300.v1.1
prunus_persica	Prupe.4G041500_v2.0.a1 Prupe.4G041700_v2.0.a1
pyrus_communis	pycom05g29530 pycom05g29560 pycom10g24890 pycom10g24910 pycom10g25140
rosa_chinensis	RchiOBHm_Chr5g0007621
rosa_laevigata	RLG00000031479
rosa_multiflora	Rmu_co8439317.1_g000001 Rmu_sc0011744.1_g000006 Rmu_sc0021005.1_g000002
rosa_roxburghii	Rroxscaffold_1G00068730
rosa_rugosa	Rorug04G0429400
rosa_samantha	Rh5AG057500 Rh5BG054400 Rh5CG064400 Rh5DG053600
rosa_wichuraiana	Rw5G006180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	951
Acc36I	ACCTGC	2 cut(s)	227, 308
Acc65I	GGTACC	2 cut(s)	738, 1800
AccB1I	GGYRCC	7 cut(s)	738, 863, 1023, 1221, 1513, 1792, 1800
AccI	GTMKAC	2 cut(s)	97, 1614
AccII	CGCG	1 cut(s)	607
AccIII	TCCGGA	1 cut(s)	382
AclWI	GGATC	4 cut(s)	533, 546, 1172, 1238
AcsI	RAATTY	3 cut(s)	1252, 1580, 1661
AcyI	GRCGYC	2 cut(s)	864, 948
AfaI	GTAC	8 cut(s)	560, 680, 740, 833, 878, 943, 1095, 1802
AfiI	CCNNNNNNNGG	9 cut(s)	903, 910, 999, 1020, 1070, 1076, 1105, 1529, 1726
AgeI	ACCGGT	2 cut(s)	1020, 1121
AgsI	TTSAA	3 cut(s)	475, 1603, 1747
AjiI	CACGTC	1 cut(s)	1753
AjnI	CCWGG	2 cut(s)	1329, 1593
AjuI	GAANNNNNNNTTGG	4 cut(s)	615, 647, 1174, 1206
AleI	CACNNNNGTG	1 cut(s)	1790
Alw21I	GWGCWC	4 cut(s)	202, 370, 579, 855
Alw26I	GTCTC	2 cut(s)	1513, 1566
Alw44I	GTGCAC	1 cut(s)	366
AlwI	GGATC	4 cut(s)	533, 546, 1172, 1238
Aor13HI	TCCGGA	1 cut(s)	382
AoxI	GGCC	4 cut(s)	237, 586, 1767, 1811
ApaLI	GTGCAC	1 cut(s)	366
ApeKI	GCWGC	4 cut(s)	168, 518, 639, 1469
ApoI	RAATTY	3 cut(s)	1252, 1580, 1661
ArsI	GACNNNNNNTTYG	4 cut(s)	110, 142, 773, 805
AsiGI	ACCGGT	2 cut(s)	1020, 1121
Asp700I	GAANNNNTTC	1 cut(s)	914
Asp718I	GGTACC	2 cut(s)	738, 1800
AspLEI	GCGC	2 cut(s)	487, 866
AspS9I	GGNCC	7 cut(s)	586, 925, 1018, 1108, 1119, 1210, 1811
AsuC2I	CCSGG	1 cut(s)	1071
AsuHPI	GGTGA	3 cut(s)	63, 1136, 1327
AsuNHI	GCTAGC	1 cut(s)	514
AvaII	GGWCC	5 cut(s)	925, 1018, 1108, 1119, 1210
BaeGI	GKGCMC	1 cut(s)	370
BaeI	ACNNNNGTAYC	4 cut(s)	670, 703, 933, 966
BamHI	GGATCC	1 cut(s)	538
BanI	GGYRCC	7 cut(s)	738, 863, 1023, 1221, 1513, 1792, 1800
BanII	GRGCYC	2 cut(s)	579, 855
BarI	GAAGNNNNNNTAC	2 cut(s)	731, 763
Bbv12I	GWGCWC	4 cut(s)	202, 370, 579, 855
BbvI	GCAGC	4 cut(s)	155, 530, 651, 1481
BccI	CCATC	3 cut(s)	644, 818, 1433
BceAI	ACGGC	2 cut(s)	207, 877
BcgI	CGANNNNNNTGC	2 cut(s)	1819, 1853
BciT130I	CCWGG	2 cut(s)	1331, 1595
BcnI	CCSGG	1 cut(s)	1071
BcoDI	GTCTC	2 cut(s)	1513, 1566
BfaI	CTAG	3 cut(s)	515, 1641, 1773
BfmI	CTRYAG	4 cut(s)	81, 174, 934, 1467
BfoI	RGCGCY	2 cut(s)	488, 867
BfuAI	ACCTGC	2 cut(s)	227, 308
BglI	GCCNNNNNGGC	1 cut(s)	1766
BisI	GCNGC	6 cut(s)	169, 400, 519, 640, 1470, 1770
BlsI	GCNGC	6 cut(s)	170, 401, 520, 641, 1471, 1771
Bme1390I	CCNGG	3 cut(s)	1071, 1331, 1595
Bme18I	GGWCC	5 cut(s)	925, 1018, 1108, 1119, 1210
BmgBI	CACGTC	1 cut(s)	1753
BmgT120I	GGNCC	7 cut(s)	586, 925, 1018, 1108, 1119, 1210, 1811
BmrFI	CCNGG	3 cut(s)	1071, 1331, 1595
BmsI	GCATC	3 cut(s)	1618, 1839, 1845
BmtI	GCTAGC	1 cut(s)	518
BpmI	CTGGAG	2 cut(s)	710, 1376
BpuMI	CCSGG	1 cut(s)	1071
BsaAI	YACGTR	2 cut(s)	1412, 1618
BsaBI	GATNNNNATC	1 cut(s)	1562
BsaHI	GRCGYC	2 cut(s)	864, 948
BsaI	GGTCTC	1 cut(s)	1513
BsaJI	CCNNGG	6 cut(s)	763, 1014, 1069, 1385, 1594, 1796
BsaWI	WCCGGW	4 cut(s)	382, 1020, 1121, 1803
BsaXI	ACNNNNNCTCC	4 cut(s)	451, 481, 851, 881
Bsc4I	CCNNNNNNNGG	9 cut(s)	903, 910, 999, 1020, 1070, 1076, 1105, 1529, 1726
Bse118I	RCCGGY	6 cut(s)	903, 999, 1020, 1121, 1169, 1813
Bse1I	ACTGG	4 cut(s)	479, 1121, 1274, 1317
Bse3DI	GCAATG	3 cut(s)	353, 634, 1506
Bse8I	GATNNNNATC	1 cut(s)	1562
BseAI	TCCGGA	1 cut(s)	382
BseBI	CCWGG	2 cut(s)	1331, 1595
BseDI	CCNNGG	6 cut(s)	763, 1014, 1069, 1385, 1594, 1796
BseJI	GATNNNNATC	1 cut(s)	1562
BseLI	CCNNNNNNNGG	9 cut(s)	903, 910, 999, 1020, 1070, 1076, 1105, 1529, 1726
BseMI	GCAATG	3 cut(s)	353, 634, 1506
BseMII	CTCAG	3 cut(s)	513, 536, 564
BseNI	ACTGG	4 cut(s)	479, 1121, 1274, 1317
BseRI	GAGGAG	3 cut(s)	309, 327, 1568
BseSI	GKGCMC	1 cut(s)	370
BseXI	GCAGC	4 cut(s)	155, 530, 651, 1481
BseYI	CCCAGC	2 cut(s)	674, 1649
BsgI	GTGCAG	2 cut(s)	1095, 1392
Bsh1236I	CGCG	1 cut(s)	607
Bsh1285I	CGRYCG	1 cut(s)	1174
BshFI	GGCC	4 cut(s)	239, 588, 1769, 1813
BshNI	GGYRCC	7 cut(s)	738, 863, 1023, 1221, 1513, 1792, 1800
BshTI	ACCGGT	2 cut(s)	1020, 1121
BsiEI	CGRYCG	1 cut(s)	1174
BsiHKAI	GWGCWC	4 cut(s)	202, 370, 579, 855
BslFI	GGGAC	2 cut(s)	827, 1670
BslI	CCNNNNNNNGG	9 cut(s)	903, 910, 999, 1020, 1070, 1076, 1105, 1529, 1726
BsmAI	GTCTC	2 cut(s)	1513, 1566
BsmFI	GGGAC	2 cut(s)	827, 1670
BsmI	GAATGC	1 cut(s)	532
BsnI	GGCC	4 cut(s)	239, 588, 1769, 1813
Bso31I	GGTCTC	1 cut(s)	1513
Bsp1286I	GDGCHC	4 cut(s)	202, 370, 579, 855
Bsp13I	TCCGGA	1 cut(s)	382
Bsp143I	GATC	6 cut(s)	538, 1177, 1230, 1456, 1500, 1563
BspANI	GGCC	4 cut(s)	239, 588, 1769, 1813
BspCNI	CTCAG	3 cut(s)	512, 535, 565
BspEI	TCCGGA	1 cut(s)	382
BspFNI	CGCG	1 cut(s)	607
BspHI	TCATGA	1 cut(s)	579
BspMAI	CTGCAG	1 cut(s)	1471
BspMI	ACCTGC	2 cut(s)	227, 308
BspOI	GCTAGC	1 cut(s)	518
BspPI	GGATC	4 cut(s)	533, 546, 1172, 1238
BspQI	GCTCTTC	2 cut(s)	559, 722
BspT107I	GGYRCC	7 cut(s)	738, 863, 1023, 1221, 1513, 1792, 1800
BspTNI	GGTCTC	1 cut(s)	1513
BsrDI	GCAATG	3 cut(s)	353, 634, 1506
BsrFI	RCCGGY	6 cut(s)	903, 999, 1020, 1121, 1169, 1813
BsrI	ACTGG	4 cut(s)	479, 1121, 1274, 1317
BssAI	RCCGGY	6 cut(s)	903, 999, 1020, 1121, 1169, 1813
BssECI	CCNNGG	6 cut(s)	763, 1014, 1069, 1385, 1594, 1796
BssMI	GATC	6 cut(s)	538, 1177, 1230, 1456, 1500, 1563
BssNAI	GTATAC	1 cut(s)	1615
BssNI	GRCGYC	2 cut(s)	864, 948
BssT1I	CCWWGG	2 cut(s)	1014, 1796
Bst1107I	GTATAC	1 cut(s)	1615
Bst2UI	CCWGG	2 cut(s)	1331, 1595
Bst4CI	ACNGT	6 cut(s)	85, 95, 818, 938, 1061, 1108
Bst6I	CTCTTC	3 cut(s)	559, 722, 749
BstACI	GRCGYC	2 cut(s)	864, 948
BstAPI	GCANNNNNTGC	2 cut(s)	197, 645
BstBAI	YACGTR	2 cut(s)	1412, 1618
BstC8I	GCNNGC	5 cut(s)	20, 45, 516, 1054, 1140
BstDEI	CTNAG	4 cut(s)	499, 522, 573, 670
BstDSI	CCRYGG	1 cut(s)	1385
BstEII	GGTNACC	1 cut(s)	1124
BstFNI	CGCG	1 cut(s)	607
BstH2I	RGCGCY	2 cut(s)	488, 867
BstHHI	GCGC	2 cut(s)	487, 866
BstKTI	GATC	6 cut(s)	541, 1180, 1233, 1459, 1503, 1566
BstMAI	GTCTC	2 cut(s)	1513, 1566
BstMBI	GATC	6 cut(s)	538, 1177, 1230, 1456, 1500, 1563
BstMCI	CGRYCG	1 cut(s)	1174
BstNI	CCWGG	2 cut(s)	1331, 1595
BstNSI	RCATGY	4 cut(s)	8, 832, 1219, 1685
BstPI	GGTNACC	1 cut(s)	1124
BstSCI	CCNGG	3 cut(s)	1069, 1329, 1593
BstSFI	CTRYAG	4 cut(s)	81, 174, 934, 1467
BstSLI	GKGCMC	1 cut(s)	370
BstUI	CGCG	1 cut(s)	607
BstV1I	GCAGC	4 cut(s)	155, 530, 651, 1481
BstX2I	RGATCY	2 cut(s)	538, 1177
BstYI	RGATCY	2 cut(s)	538, 1177
BstZ17I	GTATAC	1 cut(s)	1615
BsuRI	GGCC	4 cut(s)	239, 588, 1769, 1813
BtgI	CCRYGG	1 cut(s)	1385
BtgZI	GCGATG	1 cut(s)	663
BtrI	CACGTC	1 cut(s)	1753
BtsIMutI	CAGTG	2 cut(s)	570, 943
BveI	ACCTGC	2 cut(s)	227, 308
Cac8I	GCNNGC	5 cut(s)	20, 45, 516, 1054, 1140
CciI	TCATGA	1 cut(s)	579
CfoI	GCGC	2 cut(s)	487, 866
Cfr10I	RCCGGY	6 cut(s)	903, 999, 1020, 1121, 1169, 1813
Cfr13I	GGNCC	7 cut(s)	586, 925, 1018, 1108, 1119, 1210, 1811
Csp6I	GTAC	8 cut(s)	559, 679, 739, 832, 877, 942, 1094, 1801
CspAI	ACCGGT	2 cut(s)	1020, 1121
CspCI	CAANNNNNGTGG	2 cut(s)	919, 954
CviQI	GTAC	8 cut(s)	559, 679, 739, 832, 877, 942, 1094, 1801
DdeI	CTNAG	4 cut(s)	499, 522, 573, 670
DinI	GGCGCC	1 cut(s)	865
DpnI	GATC	6 cut(s)	540, 1179, 1232, 1458, 1502, 1565
DpnII	GATC	6 cut(s)	538, 1177, 1230, 1456, 1500, 1563
Eam1104I	CTCTTC	3 cut(s)	559, 722, 749
EarI	CTCTTC	3 cut(s)	559, 722, 749
EciI	GGCGGA	3 cut(s)	178, 429, 1559
Ecl136II	GAGCTC	2 cut(s)	577, 853
Eco130I	CCWWGG	2 cut(s)	1014, 1796
Eco24I	GRGCYC	2 cut(s)	579, 855
Eco31I	GGTCTC	1 cut(s)	1513
Eco47I	GGWCC	5 cut(s)	925, 1018, 1108, 1119, 1210
Eco53kI	GAGCTC	2 cut(s)	577, 853
Eco91I	GGTNACC	1 cut(s)	1124
EcoICRI	GAGCTC	2 cut(s)	577, 853
EcoO109I	RGGNCCY	1 cut(s)	586
EcoO65I	GGTNACC	1 cut(s)	1124
EcoRII	CCWGG	2 cut(s)	1329, 1593
EcoT14I	CCWWGG	2 cut(s)	1014, 1796
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	2 cut(s)	579, 855
EgeI	GGCGCC	1 cut(s)	865
EheI	GGCGCC	1 cut(s)	865
ErhI	CCWWGG	2 cut(s)	1014, 1796
FalI	AAGNNNNNCTT	2 cut(s)	902, 934
FaqI	GGGAC	2 cut(s)	827, 1670
FauI	CCCGC	3 cut(s)	612, 1660, 1695
FblI	GTMKAC	2 cut(s)	97, 1614
Fnu4HI	GCNGC	6 cut(s)	169, 400, 519, 640, 1470, 1770
FriOI	GRGCYC	2 cut(s)	579, 855
Fsp4HI	GCNGC	6 cut(s)	169, 400, 519, 640, 1470, 1770
FspBI	CTAG	3 cut(s)	515, 1641, 1773
GlaI	GCGC	2 cut(s)	486, 865
GluI	GCNGC	6 cut(s)	169, 400, 519, 640, 1470, 1770
GsaI	CCCAGC	2 cut(s)	678, 1653
GsuI	CTGGAG	2 cut(s)	710, 1376
HaeII	RGCGCY	2 cut(s)	488, 867
HaeIII	GGCC	4 cut(s)	239, 588, 1769, 1813
HhaI	GCGC	2 cut(s)	487, 866
Hin1I	GRCGYC	2 cut(s)	864, 948
Hin6I	GCGC	2 cut(s)	485, 864
HinP1I	GCGC	2 cut(s)	485, 864
HincII	GTYRAC	4 cut(s)	98, 598, 1324, 1378
HindII	GTYRAC	4 cut(s)	98, 598, 1324, 1378
HinfI	GANTC	6 cut(s)	759, 1334, 1449, 1482, 1493, 1731
HpaI	GTTAAC	1 cut(s)	1324
HphI	GGTGA	3 cut(s)	63, 1136, 1327
Hpy166II	GTNNAC	9 cut(s)	98, 368, 598, 1111, 1210, 1324, 1378, 1415, 1615
Hpy188I	TCNGA	3 cut(s)	153, 525, 1587
Hpy8I	GTNNAC	9 cut(s)	98, 368, 598, 1111, 1210, 1324, 1378, 1415, 1615
Hpy99I	CGWCG	4 cut(s)	99, 245, 953, 1741
HpyAV	CCTTC	9 cut(s)	148, 153, 481, 577, 736, 904, 1300, 1534, 1741
HpyCH4III	ACNGT	6 cut(s)	85, 95, 818, 938, 1061, 1108
HpyCH4IV	ACGT	8 cut(s)	594, 948, 1206, 1326, 1411, 1617, 1739, 1752
HpyCH4V	TGCA	9 cut(s)	4, 18, 43, 368, 639, 1076, 1409, 1469, 1681
HpyF3I	CTNAG	4 cut(s)	499, 522, 573, 670
HpySE526I	ACGT	8 cut(s)	594, 948, 1206, 1326, 1411, 1617, 1739, 1752
Hsp92I	GRCGYC	2 cut(s)	864, 948
HspAI	GCGC	2 cut(s)	485, 864
KasI	GGCGCC	1 cut(s)	863
Kpn2I	TCCGGA	1 cut(s)	382
KpnI	GGTACC	2 cut(s)	742, 1804
KspAI	GTTAAC	1 cut(s)	1324
Kzo9I	GATC	6 cut(s)	538, 1177, 1230, 1456, 1500, 1563
LguI	GCTCTTC	2 cut(s)	559, 722
LmnI	GCTCC	7 cut(s)	165, 188, 314, 858, 1395, 1671, 1757
Lsp1109I	GCAGC	4 cut(s)	155, 530, 651, 1481
LweI	GCATC	3 cut(s)	1618, 1839, 1845
MaeI	CTAG	3 cut(s)	515, 1641, 1773
MaeII	ACGT	8 cut(s)	594, 948, 1206, 1326, 1411, 1617, 1739, 1752
MaeIII	GTNAC	4 cut(s)	418, 1124, 1783, 1853
MalI	GATC	6 cut(s)	540, 1179, 1232, 1458, 1502, 1565
MboI	GATC	6 cut(s)	538, 1177, 1230, 1456, 1500, 1563
MboII	GAAGA	6 cut(s)	576, 709, 736, 739, 1315, 1839
MflI	RGATCY	2 cut(s)	538, 1177
MhlI	GDGCHC	4 cut(s)	202, 370, 579, 855
MluCI	AATT	8 cut(s)	282, 372, 610, 808, 842, 1252, 1580, 1661
Mly113I	GGCGCC	1 cut(s)	864
MlyI	GAGTC	3 cut(s)	1328, 1443, 1487
MmeI	TCCRAC	1 cut(s)	1227
Mph1103I	ATGCAT	1 cut(s)	6
MroI	TCCGGA	1 cut(s)	382
MroXI	GAANNNNTTC	1 cut(s)	914
MseI	TTAA	7 cut(s)	210, 281, 660, 1275, 1323, 1578, 1710
MslI	CAYNNNNRTG	2 cut(s)	1511, 1790
MspA1I	CMGCKG	1 cut(s)	735
MspR9I	CCNGG	3 cut(s)	1071, 1331, 1595
Mva1269I	GAATGC	1 cut(s)	532
MvaI	CCWGG	2 cut(s)	1331, 1595
MvnI	CGCG	1 cut(s)	607
NarI	GGCGCC	1 cut(s)	864
NciI	CCSGG	1 cut(s)	1071
NdeII	GATC	6 cut(s)	538, 1177, 1230, 1456, 1500, 1563
NheI	GCTAGC	1 cut(s)	514
NmeAIII	GCCGAG	4 cut(s)	490, 1306, 1335, 1542
NmuCI	GTSAC	3 cut(s)	1124, 1783, 1853
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	4 cut(s)	8, 832, 1219, 1685
OliI	CACNNNNGTG	1 cut(s)	1790
PagI	TCATGA	1 cut(s)	579
PciSI	GCTCTTC	2 cut(s)	559, 722
PcsI	WCGNNNNNNNCGW	2 cut(s)	1614, 1758
PctI	GAATGC	1 cut(s)	532
PdmI	GAANNNNTTC	1 cut(s)	914
PfeI	GAWTC	3 cut(s)	759, 1482, 1731
PfoI	TCCNGGA	1 cut(s)	1329
PinAI	ACCGGT	2 cut(s)	1020, 1121
PkrI	GCNGC	6 cut(s)	170, 401, 520, 641, 1471, 1771
PleI	GAGTC	3 cut(s)	1328, 1443, 1487
PluTI	GGCGCC	1 cut(s)	867
PpsI	GAGTC	3 cut(s)	1328, 1443, 1487
Ppu21I	YACGTR	2 cut(s)	1412, 1618
Psp124BI	GAGCTC	2 cut(s)	579, 855
Psp6I	CCWGG	2 cut(s)	1329, 1593
PspEI	GGTNACC	1 cut(s)	1124
PspFI	CCCAGC	2 cut(s)	674, 1649
PspGI	CCWGG	2 cut(s)	1329, 1593
PspPI	GGNCC	7 cut(s)	586, 925, 1018, 1108, 1119, 1210, 1811
PstI	CTGCAG	1 cut(s)	1471
PsuI	RGATCY	2 cut(s)	538, 1177
RsaI	GTAC	8 cut(s)	560, 680, 740, 833, 878, 943, 1095, 1802
RsaNI	GTAC	8 cut(s)	559, 679, 739, 832, 877, 942, 1094, 1801
RseI	CAYNNNNRTG	2 cut(s)	1511, 1790
SacI	GAGCTC	2 cut(s)	579, 855
SalI	GTCGAC	1 cut(s)	96
SapI	GCTCTTC	2 cut(s)	559, 722
SaqAI	TTAA	7 cut(s)	210, 281, 660, 1275, 1323, 1578, 1710
SatI	GCNGC	6 cut(s)	169, 400, 519, 640, 1470, 1770
Sau3AI	GATC	6 cut(s)	538, 1177, 1230, 1456, 1500, 1563
Sau96I	GGNCC	7 cut(s)	586, 925, 1018, 1108, 1119, 1210, 1811
SchI	GAGTC	3 cut(s)	1328, 1443, 1487
ScrFI	CCNGG	3 cut(s)	1071, 1331, 1595
SduI	GDGCHC	4 cut(s)	202, 370, 579, 855
SfaNI	GCATC	3 cut(s)	1618, 1839, 1845
SfcI	CTRYAG	4 cut(s)	81, 174, 934, 1467
SfoI	GGCGCC	1 cut(s)	865
SinI	GGWCC	5 cut(s)	925, 1018, 1108, 1119, 1210
SmiMI	CAYNNNNRTG	2 cut(s)	1511, 1790
Sse9I	AATT	8 cut(s)	282, 372, 610, 808, 842, 1252, 1580, 1661
SspDI	GGCGCC	1 cut(s)	863
SspI	AATATT	1 cut(s)	208
SspMI	CTAG	3 cut(s)	515, 1641, 1773
SstI	GAGCTC	2 cut(s)	579, 855
StyD4I	CCNGG	3 cut(s)	1069, 1329, 1593
StyI	CCWWGG	2 cut(s)	1014, 1796
TaaI	ACNGT	6 cut(s)	85, 95, 818, 938, 1061, 1108
TaiI	ACGT	8 cut(s)	597, 951, 1209, 1329, 1414, 1620, 1742, 1755
TaqI	TCGA	6 cut(s)	97, 1174, 1233, 1341, 1608, 1839
TasI	AATT	8 cut(s)	282, 372, 610, 808, 842, 1252, 1580, 1661
TatI	WGTACW	2 cut(s)	558, 831
TauI	GCSGC	2 cut(s)	402, 1772
TfiI	GAWTC	3 cut(s)	759, 1482, 1731
Tru1I	TTAA	7 cut(s)	210, 281, 660, 1275, 1323, 1578, 1710
Tru9I	TTAA	7 cut(s)	210, 281, 660, 1275, 1323, 1578, 1710
TscAI	CASTG	2 cut(s)	577, 943
TseFI	GTSAC	3 cut(s)	1124, 1783, 1853
TseI	GCWGC	4 cut(s)	168, 518, 639, 1469
Tsp45I	GTSAC	3 cut(s)	1124, 1783, 1853
TspDTI	ATGAA	6 cut(s)	65, 596, 623, 692, 1010, 1022
TspGWI	ACGGA	1 cut(s)	1374
TspRI	CASTG	2 cut(s)	577, 943
VneI	GTGCAC	1 cut(s)	366
VpaK11BI	GGWCC	5 cut(s)	925, 1018, 1108, 1119, 1210
XapI	RAATTY	3 cut(s)	1252, 1580, 1661
XceI	RCATGY	4 cut(s)	8, 832, 1219, 1685
XcmI	CCANNNNNNNNNTGG	2 cut(s)	807, 1723
XmiI	GTMKAC	2 cut(s)	97, 1614
XmnI	GAANNNNTTC	1 cut(s)	914
XspI	CTAG	3 cut(s)	515, 1641, 1773
ZraI	GACGTC	1 cut(s)	949
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.