pycom10g28040

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0016777
Physical Location & Seq
Forward (+)
28799307 .. 28800308
1002 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGGCTCGAGATGGGATATTGAAGGTGGCGATCGCTGTTTTGTTCGTCGGTGTCGCCGCTGTGAGCCTCGGAGGGGGCTTGGTTGGAGCACAAGTGTACCACGTTGTCGGAGCAGACCGCGGCTGGGATCCATCCTCCGACCTTGCTTCTTGGTCCTCGGACAAAACATTTAGGGCCGGAGATAAGATGTGGCTAGCCTACTCCACGGCGCATGGGTACATAGCTGAAGTGAAGAGCAAGGAGGAATTCGAGTCGTGCGATGTAACCAACCCAATCAGGATGTTCACGGACGGCCTGGATACCATCTCAATGGACAAGGAAGGACTCCGCTACTTCGCAAGTAGCAACGTCGAGAGCTGCAAGAATGGCCTTAAACTACACGTGGAAGTTTTGCCTCAGTCATATCAAGCTCCTGAAATGCACAACACCAACGTGGCGGAATCAGAGGGCCCTGCGTTGGTTGCAGCTAATGGGCCCACGACCCCTTCTGGTTCGGCCCATCTCACTGCAAGCTTCGTGGTGATGTCCGTTGGGTTGCTTTGTTATGTATTGGGTTTTTAG

Protein Analysis

187

Amino Acids

19.74

Weight (kDa)

5.11

Isoelectric Point (pI)

32.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 44 - 123 7.9e-17 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016777)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g01050
malus_domestica MD05G1357200.v1.1
prunus_persica Prupe.4G011400_v2.0.a1
pyrus_communis pycom10g28040
rosa_chinensis RchiOBHm_Chr5g0001591
rosa_laevigata RLG00000030945
rosa_multiflora Rmu_sc0041139.1_g000001
rosa_roxburghii Rroxscaffold_1G00074730
rosa_rugosa Rorug04G0391200
rosa_samantha Rh5AG012600 Rh5BG015500 Rh5CG013600 Rh5DG013400
rosa_wichuraiana Rw5G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 120
AciI CCGC 5 cut(s) 57, 118, 120, 328, 437
AclWI GGATC 2 cut(s) 122, 135
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 246
AcvI CACGTG 1 cut(s) 382
AfaI GTAC 2 cut(s) 98, 218
AfiI CCNNNNNNNGG 3 cut(s) 73, 124, 457
AflIII ACRYGT 1 cut(s) 379
AgsI TTSAA 1 cut(s) 22
AjnI CCWGG 1 cut(s) 294
AleI CACNNNNGTG 1 cut(s) 431
AluBI AGCT 5 cut(s) 224, 357, 410, 467, 513
AluI AGCT 5 cut(s) 224, 357, 410, 467, 513
Alw21I GWGCWC 1 cut(s) 91
AlwI GGATC 2 cut(s) 122, 135
Ama87I CYCGRG 1 cut(s) 6
AoxI GGCC 6 cut(s) 174, 292, 367, 448, 473, 495
ApaI GGGCCC 2 cut(s) 452, 477
ApeKI GCWGC 2 cut(s) 357, 464
ApoI RAATTY 1 cut(s) 245
AsiSI GCGATCGC 1 cut(s) 33
AspLEI GCGC 1 cut(s) 211
AspS9I GGNCC 7 cut(s) 153, 174, 448, 449, 473, 474, 496
AsuHPI GGTGA 1 cut(s) 532
AsuNHI GCTAGC 1 cut(s) 193
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 1 cut(s) 153
BaeGI GKGCMC 2 cut(s) 452, 477
BaeI ACNNNNGTAYC 2 cut(s) 80, 113
BamHI GGATCC 1 cut(s) 127
BanII GRGCYC 2 cut(s) 452, 477
BbrPI CACGTG 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 91
BbvI GCAGC 2 cut(s) 344, 476
BccI CCATC 4 cut(s) 5, 139, 311, 507
BceAI ACGGC 2 cut(s) 222, 307
BciT130I CCWGG 1 cut(s) 296
BciVI GTATCC 1 cut(s) 292
BfaI CTAG 1 cut(s) 194
BfuI GTATCC 1 cut(s) 292
BisI GCNGC 4 cut(s) 57, 121, 358, 465
BlsI GCNGC 4 cut(s) 58, 122, 359, 466
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 1 cut(s) 153
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 7 cut(s) 153, 174, 448, 449, 473, 474, 496
BmiI GGNNCC 3 cut(s) 129, 450, 475
BmrFI CCNGG 1 cut(s) 296
BmtI GCTAGC 1 cut(s) 197
BsaAI YACGTR 1 cut(s) 382
BsaJI CCNNGG 4 cut(s) 67, 118, 156, 204
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 124, 457
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 4 cut(s) 67, 118, 156, 204
BseGI GGATG 2 cut(s) 131, 285
BseLI CCNNNNNNNGG 3 cut(s) 73, 124, 457
BseMII CTCAG 1 cut(s) 410
BseSI GKGCMC 2 cut(s) 452, 477
BseXI GCAGC 2 cut(s) 344, 476
BseYI CCCAGC 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 120
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 6 cut(s) 176, 294, 369, 450, 475, 497
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 91
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 1 cut(s) 177
BslI CCNNNNNNNGG 3 cut(s) 73, 124, 457
BsnI GGCC 6 cut(s) 176, 294, 369, 450, 475, 497
BsoBI CYCGRG 1 cut(s) 6
Bsp120I GGGCCC 2 cut(s) 448, 473
Bsp1286I GDGCHC 3 cut(s) 91, 452, 477
Bsp143I GATC 2 cut(s) 30, 127
BspACI CCGC 5 cut(s) 57, 118, 120, 328, 437
BspANI GGCC 6 cut(s) 176, 294, 369, 450, 475, 497
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 3 cut(s) 129, 450, 475
BspOI GCTAGC 1 cut(s) 197
BspPI GGATC 2 cut(s) 122, 135
BspQI GCTCTTC 1 cut(s) 227
BssECI CCNNGG 4 cut(s) 67, 118, 156, 204
BssMI GATC 2 cut(s) 30, 127
Bst2UI CCWGG 1 cut(s) 296
Bst6I CTCTTC 1 cut(s) 227
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 2 cut(s) 195, 511
BstDEI CTNAG 1 cut(s) 396
BstDSI CCRYGG 2 cut(s) 118, 204
BstF5I GGATG 2 cut(s) 131, 285
BstFNI CGCG 1 cut(s) 120
BstHHI GCGC 1 cut(s) 211
BstKTI GATC 2 cut(s) 33, 130
BstMBI GATC 2 cut(s) 30, 127
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 2 cut(s) 366, 461
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 294
BstSLI GKGCMC 2 cut(s) 452, 477
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 2 cut(s) 344, 476
BstX2I RGATCY 1 cut(s) 127
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 1 cut(s) 127
BsuI GTATCC 1 cut(s) 292
BsuRI GGCC 6 cut(s) 176, 294, 369, 450, 475, 497
BtgI CCRYGG 2 cut(s) 118, 204
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 2 cut(s) 131, 285
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 2 cut(s) 195, 511
CfoI GCGC 1 cut(s) 211
Cfr13I GGNCC 7 cut(s) 153, 174, 448, 449, 473, 474, 496
Cfr42I CCGCGG 1 cut(s) 121
Csp6I GTAC 2 cut(s) 97, 217
CspCI CAANNNNNGTGG 2 cut(s) 498, 533
CviAII CATG 1 cut(s) 212
CviQI GTAC 2 cut(s) 97, 217
DdeI CTNAG 1 cut(s) 396
DpnI GATC 2 cut(s) 32, 129
DpnII GATC 2 cut(s) 30, 127
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
EciI GGCGGA 1 cut(s) 452
Eco24I GRGCYC 2 cut(s) 452, 477
Eco47I GGWCC 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 246
Eco72I CACGTG 1 cut(s) 382
Eco88I CYCGRG 1 cut(s) 6
EcoO109I RGGNCCY 2 cut(s) 448, 449
EcoRI GAATTC 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 294
EcoT38I GRGCYC 2 cut(s) 452, 477
FaeI CATG 1 cut(s) 215
FaiI YATR 4 cut(s) 213, 221, 403, 546
FatI CATG 1 cut(s) 211
Fnu4HI GCNGC 4 cut(s) 57, 121, 358, 465
FokI GGATG 2 cut(s) 118, 292
FriOI GRGCYC 2 cut(s) 452, 477
Fsp4HI GCNGC 4 cut(s) 57, 121, 358, 465
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 210
GluI GCNGC 4 cut(s) 57, 121, 358, 465
GsaI CCCAGC 1 cut(s) 127
HaeIII GGCC 6 cut(s) 176, 294, 369, 450, 475, 497
HapII CCGG 1 cut(s) 177
HhaI GCGC 1 cut(s) 211
Hin1II CATG 1 cut(s) 215
Hin6I GCGC 1 cut(s) 209
HinP1I GCGC 1 cut(s) 209
HindIII AAGCTT 1 cut(s) 511
HinfI GANTC 3 cut(s) 251, 324, 440
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 532
Hpy166II GTNNAC 2 cut(s) 97, 285
Hpy188I TCNGA 5 cut(s) 71, 110, 139, 160, 445
Hpy188III TCNNGA 4 cut(s) 8, 277, 352, 413
Hpy8I GTNNAC 2 cut(s) 97, 285
Hpy99I CGWCG 2 cut(s) 50, 353
HpyAV CCTTC 3 cut(s) 16, 314, 495
HpyCH4IV ACGT 4 cut(s) 102, 348, 381, 432
HpyCH4V TGCA 4 cut(s) 360, 421, 464, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 366, 461
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 4 cut(s) 102, 348, 381, 432
Hsp92II CATG 1 cut(s) 215
HspAI GCGC 1 cut(s) 209
KspI CCGCGG 1 cut(s) 121
Kzo9I GATC 2 cut(s) 30, 127
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 3 cut(s) 86, 110, 415
LpnPI CCDG 8 cut(s) 109, 190, 262, 281, 308, 426, 465, 474
Lsp1109I GCAGC 2 cut(s) 344, 476
MaeI CTAG 1 cut(s) 194
MaeII ACGT 4 cut(s) 102, 348, 381, 432
MaeIII GTNAC 1 cut(s) 262
MalI GATC 2 cut(s) 32, 129
MboI GATC 2 cut(s) 30, 127
MboII GAAGA 1 cut(s) 244
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 3 cut(s) 91, 452, 477
MluCI AATT 1 cut(s) 245
MlyI GAGTC 2 cut(s) 260, 318
MmeI TCCRAC 3 cut(s) 64, 88, 162
MnlI CCTC 7 cut(s) 65, 77, 145, 166, 235, 405, 439
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 2 cut(s) 308, 431
MspA1I CMGCKG 2 cut(s) 59, 120
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 2 cut(s) 366, 461
NdeII GATC 2 cut(s) 30, 127
NheI GCTAGC 1 cut(s) 193
NlaIII CATG 1 cut(s) 215
NlaIV GGNNCC 3 cut(s) 129, 450, 475
OliI CACNNNNGTG 1 cut(s) 431
PaeR7I CTCGAG 1 cut(s) 6
PciSI GCTCTTC 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PfeI GAWTC 1 cut(s) 440
PkrI GCNGC 4 cut(s) 58, 122, 359, 466
Ple19I CGATCG 1 cut(s) 33
PleI GAGTC 2 cut(s) 259, 318
PmaCI CACGTG 1 cut(s) 382
PmlI CACGTG 1 cut(s) 382
PpsI GAGTC 2 cut(s) 259, 318
Ppu21I YACGTR 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 294
PspCI CACGTG 1 cut(s) 382
PspFI CCCAGC 1 cut(s) 123
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 129, 450, 475
PspOMI GGGCCC 2 cut(s) 448, 473
PspPI GGNCC 7 cut(s) 153, 174, 448, 449, 473, 474, 496
PsuI RGATCY 1 cut(s) 127
PvuI CGATCG 1 cut(s) 33
RgaI GCGATCGC 1 cut(s) 33
RsaI GTAC 2 cut(s) 98, 218
RsaNI GTAC 2 cut(s) 97, 217
RseI CAYNNNNRTG 2 cut(s) 308, 431
SacII CCGCGG 1 cut(s) 121
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 4 cut(s) 57, 121, 358, 465
Sau3AI GATC 2 cut(s) 30, 127
Sau96I GGNCC 7 cut(s) 153, 174, 448, 449, 473, 474, 496
SchI GAGTC 2 cut(s) 260, 318
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 3 cut(s) 91, 452, 477
SfaAI GCGATCGC 1 cut(s) 33
Sfr274I CTCGAG 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 121
SgfI GCGATCGC 1 cut(s) 33
SgrBI CCGCGG 1 cut(s) 121
SinI GGWCC 1 cut(s) 153
SlaI CTCGAG 1 cut(s) 6
SmiMI CAYNNNNRTG 2 cut(s) 308, 431
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
Sse9I AATT 1 cut(s) 245
SsiI CCGC 5 cut(s) 57, 118, 120, 328, 437
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 1 cut(s) 294
TaiI ACGT 4 cut(s) 105, 351, 384, 435
TaqI TCGA 3 cut(s) 7, 249, 351
TasI AATT 1 cut(s) 245
TauI GCSGC 2 cut(s) 59, 123
TfiI GAWTC 1 cut(s) 440
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 1 cut(s) 511
TseI GCWGC 2 cut(s) 357, 464
TspGWI ACGGA 2 cut(s) 302, 517
TspRI CASTG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 153
XapI RAATTY 1 cut(s) 245
XhoI CTCGAG 1 cut(s) 6
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.