pycom111g02630

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0010447
Physical Location & Seq
Forward (+)
1582228 .. 1583918
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGGATTTTCTCAGGCTACTTTGTGCAGCTGTGCTTCTAGTTTTGGGGTTGCTGCTGGGAGGACAGAAGGCGGTGAATGCTGGTTCTGATGGCGGTTCCGGGAGTTGTCGCTTTCCAGCCATATACAACTTTGGGGACTCAAACTCTGACACTGGGGCTATCTCAGCTGCCATATCCGAAGTTCCTCCTCCTAATGGTGAAACCTTCTTTGGAAAACCTTCCGGGCGCTTGTCTGATGGCCGTCTTATAATCGACTTTATCGCTGAGAAGATGCAGATACCATATCTTAGTCCATATCTGGACTCACTTGGGACTAATTTCCGGCATGGTGCGAATTTCGCAACCGGTGGTTCATCGATTAGGCCAGGCGGTTATAGTCCGCCGGAGGACTTCCCGAAGGCTCTCTACACTTTCGATATTGGTCAGAATGATCTGGCCTACGGCATCCAACATGGGACGATAGAAGAAGTTAGAACCTCCATTCCTGCCATCCTGAGCCAGTTATCGCAAGCAATATCCCAACTATACAAGGAAGAAGCTAGGTTTTTCTGGGTGCATAACACAGGTCCTCAAGGATGCTTGCCCTATAGCGTTATTTACGACAAATCAAAGCCTGGTAATCTGGACAAGAGCGGCTGCGTGAAGCCTCTCAATGCGGTGGCTCAGGAGTTCAACCGACAGCTTAAGAACAGCATAATGCGGTTGAGATCACGGCTCCCTCTTGCAGCATTCACGTACGTCGATGTGTACTCGGCTAAATACACTCTCATTTCCGATGCTAAAAAACAAGGTTTTGTTGATCCTTTCGACTTTTGCTGCGGCAGTTACTACGGATTCCACATTGATTGCGGAAGGAAAGCCGTAGTGAATGGAACTGTGTATGGCAATCCTTGCAGCAGTCCGACAACGCATATTAGTTGGGATGGGATACACTACTCCCAGGCCGCCAATGCACTGATTGCCAGTCGCATTCTCAACGGCTCACTCTCTGACCCCCCAGTTTCAATCACGCACGCTTGTCACAGTTCCAAGAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0005911 GO:0009505 GO:0009506 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

347

Amino Acids

37.31

Weight (kDa)

7.56

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 324 1.2e-39 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 248
AccBSI CCGCTC 1 cut(s) 633
AclWI GGATC 1 cut(s) 794
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 1 cut(s) 334
AfaI GTAC 2 cut(s) 737, 749
AfiI CCNNNNNNNGG 1 cut(s) 194
AflII CTTAAG 1 cut(s) 683
AgeI ACCGGT 1 cut(s) 344
AgsI TTSAA 2 cut(s) 673, 1005
AjnI CCWGG 3 cut(s) 364, 613, 939
AjuI GAANNNNNNNTTGG 2 cut(s) 192, 224
AloI GAACNNNNNNTCC 2 cut(s) 466, 498
AluBI AGCT 4 cut(s) 29, 167, 539, 682
AluI AGCT 4 cut(s) 29, 167, 539, 682
AlwI GGATC 1 cut(s) 794
AoxI GGCC 4 cut(s) 238, 362, 435, 942
ApeKI GCWGC 7 cut(s) 26, 52, 167, 636, 725, 816, 894
ApoI RAATTY 1 cut(s) 334
AsiGI ACCGGT 1 cut(s) 344
Asp700I GAANNNNTTC 1 cut(s) 217
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 566
AsuC2I CCSGG 2 cut(s) 100, 223
AsuHPI GGTGA 2 cut(s) 85, 209
AvaII GGWCC 1 cut(s) 566
BarI GAAGNNNNNNTAC 2 cut(s) 389, 421
BbvI GCAGC 7 cut(s) 38, 39, 154, 623, 737, 803, 906
BccI CCATC 4 cut(s) 83, 230, 497, 917
BceAI ACGGC 5 cut(s) 225, 457, 728, 845, 994
BciT130I CCWGG 3 cut(s) 366, 615, 941
BciVI GTATCC 1 cut(s) 921
BcnI CCSGG 2 cut(s) 100, 223
BfaI CTAG 2 cut(s) 38, 540
BfmI CTRYAG 1 cut(s) 586
BfoI RGCGCY 1 cut(s) 229
BfrI CTTAAG 1 cut(s) 683
BfuI GTATCC 1 cut(s) 921
Bme1390I CCNGG 5 cut(s) 100, 223, 366, 615, 941
Bme18I GGWCC 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 566
BmiI GGNNCC 2 cut(s) 97, 716
BmrFI CCNGG 5 cut(s) 100, 223, 366, 615, 941
BmrI ACTGGG 2 cut(s) 162, 992
BmsI GCATC 4 cut(s) 261, 453, 566, 766
BmuI ACTGGG 2 cut(s) 162, 992
Bpu10I CCTNAGC 2 cut(s) 494, 663
BpuEI CTTGAG 1 cut(s) 555
BpuMI CCSGG 2 cut(s) 100, 223
Bsa29I ATCGAT 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 735
BsaJI CCNNGG 1 cut(s) 939
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 4 cut(s) 157, 499, 963, 998
BseBI CCWGG 3 cut(s) 366, 615, 941
BseCI ATCGAT 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 939
BseGI GGATG 4 cut(s) 444, 489, 581, 928
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMII CTCAG 5 cut(s) 25, 177, 255, 485, 677
BseNI ACTGG 4 cut(s) 157, 499, 963, 998
BseRI GAGGAG 1 cut(s) 177
BseXI GCAGC 7 cut(s) 38, 39, 154, 623, 737, 803, 906
BseYI CCCAGC 1 cut(s) 55
BsgI GTGCAG 1 cut(s) 45
BshFI GGCC 4 cut(s) 240, 364, 437, 944
BshTI ACCGGT 1 cut(s) 344
BshVI ATCGAT 1 cut(s) 356
BsiSI CCGG 5 cut(s) 99, 222, 322, 345, 383
BsiWI CGTACG 1 cut(s) 735
BslFI GGGAC 3 cut(s) 149, 325, 469
BslI CCNNNNNNNGG 1 cut(s) 194
BsmFI GGGAC 3 cut(s) 149, 325, 469
BsmI GAATGC 3 cut(s) 82, 728, 969
BsnI GGCC 4 cut(s) 240, 364, 437, 944
Bsp143I GATC 3 cut(s) 430, 707, 799
BspANI GGCC 4 cut(s) 240, 364, 437, 944
BspCNI CTCAG 5 cut(s) 24, 176, 256, 486, 676
BspDI ATCGAT 1 cut(s) 356
BspLI GGNNCC 2 cut(s) 97, 716
BspPI GGATC 1 cut(s) 794
BspTI CTTAAG 1 cut(s) 683
BsrBI CCGCTC 1 cut(s) 633
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 4 cut(s) 157, 499, 963, 998
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 1 cut(s) 939
BssMI GATC 3 cut(s) 430, 707, 799
Bst2UI CCWGG 3 cut(s) 366, 615, 941
Bst4CI ACNGT 2 cut(s) 877, 1025
BstAFI CTTAAG 1 cut(s) 683
BstAPI GCANNNNNTGC 2 cut(s) 891, 959
BstBAI YACGTR 1 cut(s) 735
BstC8I GCNNGC 3 cut(s) 510, 581, 1014
BstDEI CTNAG 6 cut(s) 11, 163, 264, 287, 494, 663
BstF5I GGATG 4 cut(s) 444, 489, 581, 928
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 3 cut(s) 433, 710, 802
BstMBI GATC 3 cut(s) 430, 707, 799
BstMWI GCNNNNNNNGC 6 cut(s) 77, 164, 338, 891, 950, 959
BstNI CCWGG 3 cut(s) 366, 615, 941
BstSCI CCNGG 5 cut(s) 98, 221, 364, 613, 939
BstSFI CTRYAG 1 cut(s) 586
BstV1I GCAGC 7 cut(s) 38, 39, 154, 623, 737, 803, 906
Bsu15I ATCGAT 1 cut(s) 356
BsuI GTATCC 1 cut(s) 921
BsuRI GGCC 4 cut(s) 240, 364, 437, 944
BsuTUI ATCGAT 1 cut(s) 356
BtsCI GGATG 4 cut(s) 444, 489, 581, 928
BtsIMutI CAGTG 2 cut(s) 150, 953
Cac8I GCNNGC 3 cut(s) 510, 581, 1014
CfoI GCGC 1 cut(s) 228
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 566
ClaI ATCGAT 1 cut(s) 356
Csp6I GTAC 2 cut(s) 736, 748
CspAI ACCGGT 1 cut(s) 344
CspCI CAANNNNNGTGG 2 cut(s) 827, 862
CviAII CATG 3 cut(s) 326, 452, 1038
CviQI GTAC 2 cut(s) 736, 748
DdeI CTNAG 6 cut(s) 11, 163, 264, 287, 494, 663
DpnI GATC 3 cut(s) 432, 709, 801
DpnII GATC 3 cut(s) 430, 707, 799
EaeI YGGCCR 1 cut(s) 238
EciI GGCGGA 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 566
EcoRII CCWGG 3 cut(s) 364, 613, 939
FaeI CATG 3 cut(s) 329, 455, 1041
FaqI GGGAC 3 cut(s) 149, 325, 469
FatI CATG 3 cut(s) 325, 451, 1037
FokI GGATG 4 cut(s) 431, 476, 588, 935
FspBI CTAG 2 cut(s) 38, 540
GlaI GCGC 1 cut(s) 227
GsaI CCCAGC 1 cut(s) 59
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 4 cut(s) 240, 364, 437, 944
HapII CCGG 5 cut(s) 99, 222, 322, 345, 383
HhaI GCGC 1 cut(s) 228
Hin1II CATG 3 cut(s) 329, 455, 1041
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HinfI GANTC 3 cut(s) 137, 302, 834
HpaII CCGG 5 cut(s) 99, 222, 322, 345, 383
HphI GGTGA 2 cut(s) 85, 209
Hpy166II GTNNAC 1 cut(s) 748
Hpy188I TCNGA 8 cut(s) 88, 148, 178, 235, 426, 775, 903, 991
Hpy188III TCNNGA 5 cut(s) 299, 394, 493, 623, 665
Hpy8I GTNNAC 1 cut(s) 748
Hpy99I CGWCG 1 cut(s) 743
HpyAV CCTTC 5 cut(s) 61, 214, 228, 391, 846
HpyCH4III ACNGT 2 cut(s) 877, 1025
HpyCH4IV ACGT 2 cut(s) 734, 738
HpyCH4V TGCA 6 cut(s) 26, 274, 556, 725, 894, 953
HpyF10VI GCNNNNNNNGC 6 cut(s) 77, 164, 338, 891, 950, 959
HpyF3I CTNAG 6 cut(s) 11, 163, 264, 287, 494, 663
HpySE526I ACGT 2 cut(s) 734, 738
Hsp92II CATG 3 cut(s) 329, 455, 1041
HspAI GCGC 1 cut(s) 226
Kzo9I GATC 3 cut(s) 430, 707, 799
LmnI GCTCC 1 cut(s) 720
Lsp1109I GCAGC 7 cut(s) 38, 39, 154, 623, 737, 803, 906
LweI GCATC 4 cut(s) 261, 453, 566, 766
MaeI CTAG 2 cut(s) 38, 540
MaeII ACGT 2 cut(s) 734, 738
MaeIII GTNAC 2 cut(s) 824, 1019
MalI GATC 3 cut(s) 432, 709, 801
MbiI CCGCTC 1 cut(s) 633
MboI GATC 3 cut(s) 430, 707, 799
MboII GAAGA 3 cut(s) 280, 476, 545
MluCI AATT 2 cut(s) 316, 334
MlyI GAGTC 2 cut(s) 131, 296
MmeI TCCRAC 2 cut(s) 472, 926
MnlI CCTC 8 cut(s) 53, 195, 198, 379, 487, 579, 657, 729
MroXI GAANNNNTTC 1 cut(s) 217
MseI TTAA 1 cut(s) 684
MspA1I CMGCKG 2 cut(s) 29, 167
MspCI CTTAAG 1 cut(s) 683
MspI CCGG 5 cut(s) 99, 222, 322, 345, 383
MspR9I CCNGG 5 cut(s) 100, 223, 366, 615, 941
Mva1269I GAATGC 3 cut(s) 82, 728, 969
MvaI CCWGG 3 cut(s) 366, 615, 941
MwoI GCNNNNNNNGC 6 cut(s) 77, 164, 338, 891, 950, 959
NciI CCSGG 2 cut(s) 100, 223
NdeII GATC 3 cut(s) 430, 707, 799
NlaIII CATG 3 cut(s) 329, 455, 1041
NlaIV GGNNCC 2 cut(s) 97, 716
NmeAIII GCCGAG 1 cut(s) 731
NmuCI GTSAC 1 cut(s) 1019
PctI GAATGC 3 cut(s) 82, 728, 969
PdmI GAANNNNTTC 1 cut(s) 217
PfeI GAWTC 1 cut(s) 834
Pfl23II CGTACG 1 cut(s) 735
PfoI TCCNGGA 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 344
PleI GAGTC 2 cut(s) 131, 296
PpsI GAGTC 2 cut(s) 131, 296
Ppu21I YACGTR 1 cut(s) 735
PpuMI RGGWCCY 1 cut(s) 566
PsiI TTATAA 1 cut(s) 248
Psp5II RGGWCCY 1 cut(s) 566
Psp6I CCWGG 3 cut(s) 364, 613, 939
PspFI CCCAGC 1 cut(s) 55
PspGI CCWGG 3 cut(s) 364, 613, 939
PspLI CGTACG 1 cut(s) 735
PspN4I GGNNCC 2 cut(s) 97, 716
PspPI GGNCC 1 cut(s) 566
PspPPI RGGWCCY 1 cut(s) 566
PvuII CAGCTG 2 cut(s) 29, 167
RsaI GTAC 2 cut(s) 737, 749
RsaNI GTAC 2 cut(s) 736, 748
SaqAI TTAA 1 cut(s) 684
Sau3AI GATC 3 cut(s) 430, 707, 799
Sau96I GGNCC 1 cut(s) 566
SchI GAGTC 2 cut(s) 131, 296
ScrFI CCNGG 5 cut(s) 100, 223, 366, 615, 941
SfaNI GCATC 4 cut(s) 261, 453, 566, 766
SfcI CTRYAG 1 cut(s) 586
SinI GGWCC 1 cut(s) 566
SmlI CTYRAG 2 cut(s) 570, 683
SmoI CTYRAG 2 cut(s) 570, 683
Sse9I AATT 2 cut(s) 316, 334
SspMI CTAG 2 cut(s) 38, 540
StyD4I CCNGG 5 cut(s) 98, 221, 364, 613, 939
TaaI ACNGT 2 cut(s) 877, 1025
TaiI ACGT 2 cut(s) 737, 741
TaqI TCGA 5 cut(s) 252, 356, 414, 741, 807
TasI AATT 2 cut(s) 316, 334
TatI WGTACW 1 cut(s) 747
TauI GCSGC 3 cut(s) 636, 822, 947
TfiI GAWTC 1 cut(s) 834
Tru1I TTAA 1 cut(s) 684
Tru9I TTAA 1 cut(s) 684
TscAI CASTG 2 cut(s) 157, 960
TseFI GTSAC 1 cut(s) 1019
TseI GCWGC 7 cut(s) 26, 52, 167, 636, 725, 816, 894
Tsp45I GTSAC 1 cut(s) 1019
TspDTI ATGAA 1 cut(s) 342
TspGWI ACGGA 1 cut(s) 846
TspRI CASTG 2 cut(s) 157, 960
Vha464I CTTAAG 1 cut(s) 683
VpaK11BI GGWCC 1 cut(s) 566
XapI RAATTY 1 cut(s) 334
XmnI GAANNNNTTC 1 cut(s) 217
XspI CTAG 2 cut(s) 38, 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.