pycom11g00410

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0014912
Physical Location & Seq
Forward (+)
321048 .. 322873
1826 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGAGAGAAATTATAAGCATTCATATTGGGCAGGCTGGAATTCAGGTGGGGAATTCATGCTGGGAGCTCTACTGCCTCGAGCATGGAATTCAACCTGATGGGCAGATGCCCAGTGATACAACAGTGGGTAGCGGCCGCGATGCTTTCAATACCTTCTTCAGTGAGACTGGATCAGGCAAGCATGTCCCTAGGGCCATATTTGTCGATTTGGAACCAACTGTTATTGATGAAGTAAGGACTGGGTCTTACCGCCAACTCTTTCACCCCGAGCAGCTCATTTCTGGAAAGGAAGATGCTGCTAATAATTTCGCAAGAGGACACTATACAGTTGGAAAGGAAATTGTAGATCTCTGCCTTGATCGAGTTAGGAAATTGGCTGATAATTGCACTGGTTTACAAGGATTTTTGGTGTTTAATGCTGTGGGTGGTGGTACCGGTTCTGGTTTGGGGTCCTTGCTGTTAGAACGCTTGTCCGTGGATTATGGAAAGAAGTCAAAGCTTGGATTCACCATCTATCCTTCTCCACAGGTTTCAACAGCAGTTGTCGAGCCTTATAATAGCGTTCTCTCTACTCATGCTCTCCTTGAACACACCGATGTATCTGTGCTCTTGGACAATGAAGCTATCTATGATATTTGCAGAAGATCGATCTAG

Protein Analysis

218

Amino Acids

23.63

Weight (kDa)

5.31

Isoelectric Point (pI)

28.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tubulin PF00091 3 - 213 8e-64 Tubulin/FtsZ family, GTPase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19770 AT5G19780
fragaria_vesca FvH4_3g45490
malus_domestica MD03G1004400.v1.1 MD11G1005100.v1.1
prunus_persica Prupe.6G004100_v2.0.a1 Prupe.6G004100_v2.0.a1
pyrus_communis pycom03g00450 pycom11g00410
rosa_chinensis RchiOBHm_Chr5g0083281
rosa_laevigata RLG00000037026
rosa_multiflora Rmu_sc0011792.1_g000008
rosa_roxburghii Rroxscaffold_1G00001210
rosa_rugosa Rorug05G0484000
rosa_samantha Rh5BG560700
rosa_wichuraiana Rw5G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 14, 555
Acc65I GGTACC 1 cut(s) 431
AccB1I GGYRCC 1 cut(s) 431
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 132, 136, 250
AclWI GGATC 1 cut(s) 178
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 3 cut(s) 39, 52, 87
AcuI CTGAAG 1 cut(s) 142
AfaI GTAC 1 cut(s) 433
AgeI ACCGGT 1 cut(s) 434
AgsI TTSAA 4 cut(s) 92, 148, 534, 587
AluBI AGCT 4 cut(s) 67, 274, 499, 623
AluI AGCT 4 cut(s) 67, 274, 499, 623
Alw21I GWGCWC 2 cut(s) 69, 609
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 1 cut(s) 178
Ama87I CYCGRG 2 cut(s) 77, 266
AoxI GGCC 2 cut(s) 133, 192
ApeKI GCWGC 2 cut(s) 271, 296
ApoI RAATTY 3 cut(s) 39, 52, 87
AsiGI ACCGGT 1 cut(s) 434
Asp718I GGTACC 1 cut(s) 431
AspA2I CCTAGG 1 cut(s) 188
AspS9I GGNCC 2 cut(s) 192, 450
AsuHPI GGTGA 2 cut(s) 254, 499
AvaI CYCGRG 2 cut(s) 77, 266
AvaII GGWCC 1 cut(s) 450
AvrII CCTAGG 1 cut(s) 188
BaeI ACNNNNGTAYC 4 cut(s) 108, 141, 582, 615
BanI GGYRCC 1 cut(s) 431
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 69, 609
BbvI GCAGC 2 cut(s) 283, 283
BccI CCATC 2 cut(s) 92, 518
BcoDI GTCTC 1 cut(s) 158
BfaI CTAG 2 cut(s) 189, 652
BglII AGATCT 1 cut(s) 346
BisI GCNGC 4 cut(s) 133, 136, 272, 297
BlnI CCTAGG 1 cut(s) 188
BlsI GCNGC 4 cut(s) 134, 137, 273, 298
Bme18I GGWCC 1 cut(s) 450
BmeT110I CYCGRG 2 cut(s) 77, 266
BmgT120I GGNCC 2 cut(s) 192, 450
BmiI GGNNCC 3 cut(s) 213, 433, 451
BmrI ACTGGG 2 cut(s) 105, 249
BmsI GCATC 3 cut(s) 96, 130, 283
BmuI ACTGGG 2 cut(s) 105, 249
Bsa29I ATCGAT 1 cut(s) 647
BsaJI CCNNGG 2 cut(s) 188, 474
BsaWI WCCGGW 1 cut(s) 434
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 4 cut(s) 111, 172, 244, 394
BseCI ATCGAT 1 cut(s) 647
BseDI CCNNGG 2 cut(s) 188, 474
BseNI ACTGG 4 cut(s) 111, 172, 244, 394
BseX3I CGGCCG 1 cut(s) 133
BseXI GCAGC 2 cut(s) 283, 283
BseYI CCCAGC 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 2 cut(s) 135, 194
BshNI GGYRCC 1 cut(s) 431
BshTI ACCGGT 1 cut(s) 434
BshVI ATCGAT 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 2 cut(s) 69, 609
BsiHKCI CYCGRG 2 cut(s) 77, 266
BsiSI CCGG 1 cut(s) 435
BslFI GGGAC 1 cut(s) 170
BsmAI GTCTC 1 cut(s) 158
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 18
BsnI GGCC 2 cut(s) 135, 194
BsoBI CYCGRG 2 cut(s) 77, 266
Bsp1286I GDGCHC 2 cut(s) 69, 609
Bsp143I GATC 5 cut(s) 170, 346, 358, 644, 648
BspACI CCGC 3 cut(s) 132, 136, 250
BspANI GGCC 2 cut(s) 135, 194
BspDI ATCGAT 1 cut(s) 647
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 3 cut(s) 213, 433, 451
BspPI GGATC 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 431
BsrFI RCCGGY 1 cut(s) 434
BsrI ACTGG 4 cut(s) 111, 172, 244, 394
BssAI RCCGGY 1 cut(s) 434
BssECI CCNNGG 2 cut(s) 188, 474
BssMI GATC 5 cut(s) 170, 346, 358, 644, 648
BssT1I CCWWGG 1 cut(s) 188
Bst4CI ACNGT 3 cut(s) 124, 220, 328
BstC8I GCNNGC 2 cut(s) 33, 179
BstDSI CCRYGG 1 cut(s) 474
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 5 cut(s) 173, 349, 361, 647, 651
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 5 cut(s) 170, 346, 358, 644, 648
BstMCI CGRYCG 1 cut(s) 136
BstNSI RCATGY 1 cut(s) 185
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 283, 283
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BstZI CGGCCG 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 647
BsuRI GGCC 2 cut(s) 135, 194
BsuTUI ATCGAT 1 cut(s) 647
BtgI CCRYGG 1 cut(s) 474
BtgZI GCGATG 1 cut(s) 153
BtsIMutI CAGTG 4 cut(s) 118, 129, 166, 387
Cac8I GCNNGC 2 cut(s) 33, 179
CciNI GCGGCCGC 1 cut(s) 133
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 192, 450
ClaI ATCGAT 1 cut(s) 647
Csp6I GTAC 1 cut(s) 432
CspAI ACCGGT 1 cut(s) 434
CviAII CATG 4 cut(s) 57, 83, 182, 575
CviJI RGCY 9 cut(s) 35, 67, 135, 194, 274, 377, 499, 550, 623
CviKI_1 RGCY 9 cut(s) 35, 67, 135, 194, 274, 377, 499, 550, 623
CviQI GTAC 1 cut(s) 432
DpnI GATC 5 cut(s) 172, 348, 360, 646, 650
DpnII GATC 5 cut(s) 170, 346, 358, 644, 648
EaeI YGGCCR 1 cut(s) 133
EagI CGGCCG 1 cut(s) 133
Ecl136II GAGCTC 1 cut(s) 67
EclXI CGGCCG 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 450
Eco52I CGGCCG 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 67
Eco57I CTGAAG 1 cut(s) 142
Eco88I CYCGRG 2 cut(s) 77, 266
EcoICRI GAGCTC 1 cut(s) 67
EcoO109I RGGNCCY 1 cut(s) 450
EcoRI GAATTC 3 cut(s) 39, 52, 87
EcoT14I CCWWGG 1 cut(s) 188
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 4 cut(s) 60, 86, 185, 578
FaqI GGGAC 1 cut(s) 170
FatI CATG 4 cut(s) 56, 82, 181, 574
Fnu4HI GCNGC 4 cut(s) 133, 136, 272, 297
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 4 cut(s) 133, 136, 272, 297
FspBI CTAG 2 cut(s) 189, 652
GluI GCNGC 4 cut(s) 133, 136, 272, 297
GsaI CCCAGC 1 cut(s) 64
HaeIII GGCC 2 cut(s) 135, 194
HapII CCGG 1 cut(s) 435
Hin1II CATG 4 cut(s) 60, 86, 185, 578
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 1 cut(s) 504
HpaII CCGG 1 cut(s) 435
HphI GGTGA 2 cut(s) 254, 499
Hpy166II GTNNAC 1 cut(s) 395
Hpy188III TCNNGA 1 cut(s) 282
Hpy8I GTNNAC 1 cut(s) 395
HpyAV CCTTC 2 cut(s) 163, 528
HpyCH4III ACNGT 3 cut(s) 124, 220, 328
HpyCH4V TGCA 2 cut(s) 387, 639
Hsp92II CATG 4 cut(s) 60, 86, 185, 578
KpnI GGTACC 1 cut(s) 435
Kzo9I GATC 5 cut(s) 170, 346, 358, 644, 648
LmnI GCTCC 1 cut(s) 64
Lsp1109I GCAGC 2 cut(s) 283, 283
LweI GCATC 3 cut(s) 96, 130, 283
MaeI CTAG 2 cut(s) 189, 652
MalI GATC 5 cut(s) 172, 348, 360, 646, 650
MboI GATC 5 cut(s) 170, 346, 358, 644, 648
MboII GAAGA 3 cut(s) 148, 302, 654
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 2 cut(s) 69, 609
MluCI AATT 8 cut(s) 9, 39, 52, 87, 304, 339, 371, 382
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 2 cut(s) 86, 308
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 1 cut(s) 594
MspI CCGG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 18
MvnI CGCG 1 cut(s) 138
NdeII GATC 5 cut(s) 170, 346, 358, 644, 648
NlaIII CATG 4 cut(s) 60, 86, 185, 578
NlaIV GGNNCC 3 cut(s) 213, 433, 451
NotI GCGGCCGC 1 cut(s) 133
NspI RCATGY 1 cut(s) 185
PaeR7I CTCGAG 1 cut(s) 77
PctI GAATGC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 504
PflFI GACNNNGTC 1 cut(s) 241
PinAI ACCGGT 1 cut(s) 434
PkrI GCNGC 4 cut(s) 134, 137, 273, 298
PpuMI RGGWCCY 1 cut(s) 450
PsiI TTATAA 2 cut(s) 14, 555
Psp124BI GAGCTC 1 cut(s) 69
Psp5II RGGWCCY 1 cut(s) 450
PspFI CCCAGC 1 cut(s) 60
PspN4I GGNNCC 3 cut(s) 213, 433, 451
PspPI GGNCC 2 cut(s) 192, 450
PspPPI RGGWCCY 1 cut(s) 450
PspXI VCTCGAGB 1 cut(s) 77
PsuI RGATCY 1 cut(s) 346
PsyI GACNNNGTC 1 cut(s) 241
RsaI GTAC 1 cut(s) 433
RsaNI GTAC 1 cut(s) 432
RseI CAYNNNNRTG 1 cut(s) 594
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 4 cut(s) 133, 136, 272, 297
Sau3AI GATC 5 cut(s) 170, 346, 358, 644, 648
Sau96I GGNCC 2 cut(s) 192, 450
SduI GDGCHC 2 cut(s) 69, 609
SetI ASST 8 cut(s) 48, 69, 97, 155, 276, 501, 531, 625
SfaNI GCATC 3 cut(s) 96, 130, 283
Sfr274I CTCGAG 1 cut(s) 77
SinI GGWCC 1 cut(s) 450
SlaI CTCGAG 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 594
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
Sse9I AATT 8 cut(s) 9, 39, 52, 87, 304, 339, 371, 382
SsiI CCGC 3 cut(s) 132, 136, 250
SspMI CTAG 2 cut(s) 189, 652
SstI GAGCTC 1 cut(s) 69
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 124, 220, 328
TaqI TCGA 5 cut(s) 78, 204, 361, 546, 647
TasI AATT 8 cut(s) 9, 39, 52, 87, 304, 339, 371, 382
TauI GCSGC 2 cut(s) 135, 138
TfiI GAWTC 1 cut(s) 504
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 4 cut(s) 118, 129, 166, 394
TseI GCWGC 2 cut(s) 271, 296
TspDTI ATGAA 4 cut(s) 11, 45, 243, 633
TspGWI ACGGA 1 cut(s) 463
TspRI CASTG 4 cut(s) 118, 129, 166, 394
Tth111I GACNNNGTC 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 3 cut(s) 39, 52, 87
XceI RCATGY 1 cut(s) 185
XhoI CTCGAG 1 cut(s) 77
XmaJI CCTAGG 1 cut(s) 188
XspI CTAG 2 cut(s) 189, 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.