pycom11g01150

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0016299
Physical Location & Seq
Forward (+)
924853 .. 929792
4940 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3381 bp
ATGGCGTCGTCTTTGCAGGATCTTTTAGCAGAAGATGGATTTAGGGGAAAAAAATTGTTGAGCAAATCAAAGGCTTCCTCCTTCCACTCAGGTTCAACTTCGCGTCATTTGGCTAATTCAGATCCACACAAGAGACGTTCCATGTCAGGGGATAGAATTAGGACAGAGAAAACAAGGTCTGATGTGTCTCGGTTTGCTGTGAATAATTATCTTCCAACGGGTGATGGTGTTGCAGGTAGGAGACCATCCGTTGATGGAGGATCAAAGAAAGAAGTTAGGGATGGACTCAGAGAAACAGGTGCTACTAGGTTGAGGGAAGCTAGAAGTTTGAATAGAACTTTTCCGTGGAGTGTGCCACGAAATGAGATAGTTGAGGTTGAAGGTGAAGATTTTGAGAGATACAAGGATATATATTCGAACGAATTGTATAGCTCAGAAAATAGAAAGGATAAGTATTTGATCGGAAGTATGGAAGATGATGGACTTGAAGAGAGGTCAAAGAAGGACACGGTGTTTGATAGGAGGAATGGCCACAGTTCAAACAAATATGTTCCTGGACGTACGAGTTTTAGCGACAGCAATAGACAAAGCAGGAAACTTCCAGAGACCTCACATGACAGATCAAGGAGAGATTCATCATATGGCAAAACTTCGGAAGATGTTTGCGGACAAAAGCGTGATAAAGTTTTACGGGGAGTTCCTGAACCTGCTCTTGATGAAATTGCTATCCAAGCCATTGTTTCCATTCTGAGTGGATGCATTAAGCAGTTTCTCAAAGATGAGAATTTCCGGCATGAACTCAGAGACAAATGTATCTCCTTCCTAAACTTTGTTGATCCGGAAGAAGGGAATGCCGAGAGCAGAATCATTGCCAGATTTGAACAGGCAATCGAAACAGTGGAAAAGGCTGCAGAGGAGTCAGCAAGCGAATGGGATTTAAAACGAGCTTCATTGCAGTTTAGTGTTATAACCGGGTTGAACAATGCCGATTTGACAGATGGATTGACAGCCGGAGTTCCTAATTACAAACTGTCAGCCTGTGCTCATCTCTATCTCAGTGTGGTGTACAAGTTACTGAAGAAAGACCGTGTATCAGCAAAGCATCTTTTGCAAGTTTTTTGTGATACACCCTTTCACGCACGAATGACATTGTTGCCTGAATTATGGGATCGACTATTTCTTCCACATTTGACACATTTAAAGACGTGGTATGATCAGGAAGCTGATTCAATAGCGGATAGACAAAACAAGCCGAGGAAGCTGAAGCTTTTAGGAAAAATGTACAATGAAATGTTGGATTCTGGGACTTACCAATTTGCCGTTTACTATAAGGACTGGCTCACCGAAGGAGTTGAATCTCCTCCCACTCCTGCTATCCATATCCCATCTGTAACATCTCTTCAGGAAGCTCAGCAGGGAAGTTCTGAAAGTCATTCTTCGGAAGCACCTAGTCCAGGCGGTCCACAGCTAATGGTCAGCAAAAAACTATACGATTCTGTGTTTAGCCGTGCAAGTAAGCCTGATCCTAATGATACCGAGGATGACGGAGAGATGGAAAACTTGGATACTTGTACGAGAAGTTCTGATGGTTCATTTGTTGTGAAACAAATAACTCAGGACTCTGCCGAAACAGTTCAATATCAGTACCACGACACTGAAGATGATTACTCTAAGAGTGCGCCGGGAGATGGATCCCTTTCTGTGTCATTGTCATGCTCAAAAACATTTTTGAAGGAAAATGGACTCTTCGCGACAGAAGAGCAAAAACGGGGTTATCATGGAGTGAGTGATCTATCAGAAAGCAACCTAAATCATCAGTTCGGTGACATGTCTGGAGAAAACTCAGAAGGCCCCCGTATGCTGGATGCACAGGAGAATAAGCTCACCCTCAAAATCCATGTAAACTCCGTTTTTGAACAGAAGCCCATGGAGGATTCCAGCGATCACAATTTATCTACTATATCGAATTTCAGTGAACCTTCGATTGCCAGTTCTATTCCAATCAAAGCAAGGTCTTCGTTCGATGGTGAGTCAAAAGGAACGTCTGAGGTTTTATATAGTTTGGTAGTGCACATCTGGATCTGTTGCTCATATTTACATTTCCTTGGCACAGATTTACATGGAAATTATTTTGAAGAAGGAGTCACTTTCTCGGGCATTCCTCAGGACTTCATTTGCCCTCTAAATGGACGGTTGTTTGAAGATCCAGTGACCCTAGAGACGGGACAAACCTTTGAGCGTTTGGCCATCAAGACGTGGCTTGATCAGGGAAACAGAACATGTCCGGTGACAGGAAAAGCTTTGGAATGTCTAGTGTTGCCTCTTCCTAACTCTATCTTAAAGCGTGTGATTCGTAGTTGGAAGTCTGAACATTGTCGGAAACTCTTAGCTTTTGCCTCTCATGTGGTGGGGACCTTGGTGAGAGATGTGTCCAGTCACTGCGACGAGACTGCTATTTCCGTGCTAGAGCAGCTTCTCACTTGTTTCAGCAATGAGGAAAGGACAGAAAATGCCAAGCACCTGATCTCTCTTGGAGGCTTGCAGTTTCTTCTTCAGCGGTTTCATTACGGAAAAGTTGAAGAGAAATCGCGTGCGGCAGCATTACTGTTGTGTTGCATAGAAGCAGATGCAGATTGTAGGAATCAAATAGCAAGAGACATTAACAAACAGTATGTTATGGAGATGCTCCAAAGCCAGCAATTTAAAATAAGAACAAATGCAGTGTTGTTGTTGACAGAATTGATTTGCCTCAAGAGATTGAGGGGATGGTGGATACAATGGATGTTCTGCTTGCGTGTCTCCAGAGTAGAGAGGAAGCTGTCGACGCCATTACACAGAGAAGAAGCTGTCGACGCCATTACAGAGGCTCTTGATTGCAGCCTAACTGATGAGAACGTCCAAAAGAATTGTTGCACAGCACTTCTTATCTTGGGAAGGTTTTTCTCTTGCTCCAGAAAATCATCACAAAGTTGGATCTTAAAGCAAGAAGATTATAGTGGCAATTCTGAAGTGAATTCACTCGACAACGAAGAAGACATTTCACTAGCTGATGACATGTCTCCATTGGATGAGGGAGAAAATTCCAGTGAAGATTGGTTGAGGAACTTGACAGTGTCACTACTCGGTTATGGAAAGCAGTCTTTTGTAGAAACCCTTTCAAAGTGTTTAGGATCCGAAAACTTGGAATTGATTAGAATGTGCCTAATCACCGTGGAATGGTTGAGCCGCGCACTTTCTTCACTGTCTGGTTCTGAGTTTCAGCTATCCGCCTTCTCATCTCTCATTTTTTCACTAAAACAATGTCTGAAGACCAGCGAGCAGGTTGAGCAGAAGATCCTTGCTTCAATGTCCATGCTCAACTTCAGCAAAATATCCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

1127

Amino Acids

126.65

Weight (kDa)

5.63

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 242 - 463 2.9e-96 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 720 - 791 2.9e-12 U-box domain
ARM_LIN_C PF23628 824 - 919 1.8e-35 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
ARM_LIN_C PF23628 944 - 1125 9.9e-56 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016299)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g44280
malus_domestica MD11G1016300.v1.1
prunus_persica Prupe.6G014500_v2.0.a1
pyrus_communis pycom11g01150
rosa_chinensis RchiOBHm_Chr5g0080651
rosa_laevigata RLG00000036879
rosa_multiflora Rmu_sc0002380.1_g000007 Rmu_sc0039404.1_g000001
rosa_roxburghii Rroxscaffold_1G00002750
rosa_rugosa Rorug05G0470900
rosa_samantha Rh5AG524200 Rh5BG546500 Rh5CG569700 Rh5DG556700
rosa_wichuraiana Rw5G048630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 968
Acc36I ACCTGC 3 cut(s) 224, 715, 3310
AccI GTMKAC 2 cut(s) 2822, 2850
AccII CGCG 4 cut(s) 103, 1751, 2590, 3228
AccIII TCCGGA 1 cut(s) 838
AciI CCGC 7 cut(s) 666, 1235, 1458, 2557, 2594, 3226, 3267
AcoI YGGCCR 2 cut(s) 529, 2244
AcsI RAATTY 4 cut(s) 784, 1966, 3013, 3079
AcuI CTGAAG 8 cut(s) 1097, 1283, 1385, 1677, 2537, 3027, 3326, 3346
AcyI GRCGYC 3 cut(s) 5, 2825, 2853
AfaI GTAC 5 cut(s) 562, 1067, 1283, 1573, 1646
AfiI CCNNNNNNNGG 9 cut(s) 147, 845, 1454, 1688, 1861, 2186, 2221, 2291, 3216
AflIII ACRYGT 3 cut(s) 1827, 2279, 3054
AjiI CACGTC 2 cut(s) 1206, 2256
AjnI CCWGG 2 cut(s) 553, 1453
Alw21I GWGCWC 2 cut(s) 1045, 2073
Alw44I GTGCAC 1 cut(s) 2069
AlwNI CAGNNNCTG 1 cut(s) 2439
Ama87I CYCGRG 1 cut(s) 2152
Aor13HI TCCGGA 1 cut(s) 838
AoxI GGCC 3 cut(s) 529, 1849, 2244
ApaLI GTGCAC 1 cut(s) 2069
ApeKI GCWGC 4 cut(s) 908, 2470, 2597, 2877
ApoI RAATTY 4 cut(s) 784, 1966, 3013, 3079
ArsI GACNNNNNNTTYG 4 cut(s) 497, 529, 2027, 2059
Asp700I GAANNNNTTC 1 cut(s) 1632
AspLEI GCGC 2 cut(s) 1681, 3230
AspS9I GGNCC 3 cut(s) 1460, 1850, 2412
AsuC2I CCSGG 2 cut(s) 973, 1683
AsuHPI GGTGA 9 cut(s) 233, 395, 1333, 1835, 1876, 2039, 2299, 2431, 3199
AsuII TTCGAA 1 cut(s) 416
AvaI CYCGRG 1 cut(s) 2152
AvaII GGWCC 2 cut(s) 1460, 2412
AxyI CCTNAGG 1 cut(s) 2163
BaeGI GKGCMC 1 cut(s) 2073
BaeI ACNNNNGTAYC 2 cut(s) 796, 829
BalI TGGCCA 2 cut(s) 531, 2246
BamHI GGATCC 2 cut(s) 1691, 3170
BarI GAAGNNNNNNTAC 2 cut(s) 1383, 1415
BbsI GAAGAC 3 cut(s) 2007, 3039, 3314
Bbv12I GWGCWC 2 cut(s) 1045, 2073
BbvI GCAGC 4 cut(s) 895, 2482, 2609, 2889
BceAI ACGGC 2 cut(s) 1304, 1491
BcgI CGANNNNNNTGC 4 cut(s) 1998, 2032, 2432, 2466
BciT130I CCWGG 2 cut(s) 555, 1455
BciVI GTATCC 2 cut(s) 1558, 2766
BclI TGATCA 2 cut(s) 1213, 2263
BcnI CCSGG 2 cut(s) 973, 1683
BfaI CTAG 7 cut(s) 306, 321, 1449, 2216, 2312, 2465, 3044
BfmI CTRYAG 1 cut(s) 909
BfuAI ACCTGC 3 cut(s) 224, 715, 3310
BfuI GTATCC 2 cut(s) 1558, 2766
BisI GCNGC 6 cut(s) 909, 2471, 2595, 2598, 2878, 3226
BlpI GCTNAGC 1 cut(s) 1410
BlsI GCNGC 6 cut(s) 910, 2472, 2596, 2599, 2879, 3227
Bme1390I CCNGG 4 cut(s) 555, 973, 1455, 1683
Bme18I GGWCC 2 cut(s) 1460, 2412
BmeT110I CYCGRG 1 cut(s) 2152
BmgBI CACGTC 2 cut(s) 1206, 2256
BmgT120I GGNCC 3 cut(s) 1460, 1850, 2412
BmiI GGNNCC 4 cut(s) 1693, 1852, 2413, 3172
BmrFI CCNGG 4 cut(s) 555, 973, 1455, 1683
BmsI GCATC 5 cut(s) 746, 1111, 1855, 2617, 2673
BpiI GAAGAC 3 cut(s) 2007, 3039, 3314
BpmI CTGGAG 3 cut(s) 1854, 2785, 2935
Bpu1102I GCTNAGC 1 cut(s) 1410
Bpu14I TTCGAA 1 cut(s) 416
BpuEI CTTGAG 1 cut(s) 2735
BpuMI CCSGG 2 cut(s) 973, 1683
BsaHI GRCGYC 3 cut(s) 5, 2825, 2853
BsaI GGTCTC 2 cut(s) 235, 599
BsaJI CCNNGG 7 cut(s) 344, 1253, 1536, 1926, 2104, 2415, 3210
BsaWI WCCGGW 3 cut(s) 838, 2284, 3374
Bsc4I CCNNNNNNNGG 9 cut(s) 147, 845, 1454, 1688, 1861, 2186, 2221, 2291, 3216
Bse1I ACTGG 5 cut(s) 1340, 1989, 2207, 2433, 3084
Bse21I CCTNAGG 1 cut(s) 2163
Bse3DI GCAATG 3 cut(s) 867, 950, 2497
BseAI TCCGGA 1 cut(s) 838
BseBI CCWGG 2 cut(s) 555, 1455
BseDI CCNNGG 7 cut(s) 344, 1253, 1536, 1926, 2104, 2415, 3210
BseGI GGATG 8 cut(s) 245, 286, 761, 1546, 1870, 2771, 2787, 3073
BseLI CCNNNNNNNGG 9 cut(s) 147, 845, 1454, 1688, 1861, 2186, 2221, 2291, 3216
BseMI GCAATG 3 cut(s) 867, 950, 2497
BseNI ACTGG 5 cut(s) 1340, 1989, 2207, 2433, 3084
BseRI GAGGAG 2 cut(s) 929, 1350
BseSI GKGCMC 1 cut(s) 2073
BseXI GCAGC 4 cut(s) 895, 2482, 2609, 2889
Bsh1236I CGCG 4 cut(s) 103, 1751, 2590, 3228
BshFI GGCC 3 cut(s) 531, 1851, 2246
BsiHKAI GWGCWC 2 cut(s) 1045, 2073
BsiHKCI CYCGRG 1 cut(s) 2152
BsiSI CCGG 7 cut(s) 790, 839, 972, 1011, 1682, 2285, 3375
BsiWI CGTACG 1 cut(s) 560
BslFI GGGAC 3 cut(s) 1318, 2238, 2425
BslI CCNNNNNNNGG 9 cut(s) 147, 845, 1454, 1688, 1861, 2186, 2221, 2291, 3216
BsmBI CGTCTC 2 cut(s) 127, 2213
BsmFI GGGAC 3 cut(s) 1318, 2238, 2425
BsmI GAATGC 2 cut(s) 856, 2157
BsnI GGCC 3 cut(s) 531, 1851, 2246
Bso31I GGTCTC 2 cut(s) 235, 599
BsoBI CYCGRG 1 cut(s) 2152
Bsp119I TTCGAA 1 cut(s) 416
Bsp1286I GDGCHC 2 cut(s) 1045, 2073
Bsp13I TCCGGA 1 cut(s) 838
Bsp1407I TGTACA 2 cut(s) 1065, 1281
Bsp1720I GCTNAGC 1 cut(s) 1410
Bsp19I CCATGG 1 cut(s) 1926
Bsp68I TCGCGA 1 cut(s) 1751
BspACI CCGC 7 cut(s) 666, 1235, 1458, 2557, 2594, 3226, 3267
BspANI GGCC 3 cut(s) 531, 1851, 2246
BspEI TCCGGA 1 cut(s) 838
BspFNI CGCG 4 cut(s) 103, 1751, 2590, 3228
BspLI GGNNCC 4 cut(s) 1693, 1852, 2413, 3172
BspMAI CTGCAG 1 cut(s) 913
BspMI ACCTGC 3 cut(s) 224, 715, 3310
BspQI GCTCTTC 1 cut(s) 1752
BspT104I TTCGAA 1 cut(s) 416
BspTNI GGTCTC 2 cut(s) 235, 599
BsrDI GCAATG 3 cut(s) 867, 950, 2497
BsrGI TGTACA 2 cut(s) 1065, 1281
BsrI ACTGG 5 cut(s) 1340, 1989, 2207, 2433, 3084
BssECI CCNNGG 7 cut(s) 344, 1253, 1536, 1926, 2104, 2415, 3210
BssNI GRCGYC 3 cut(s) 5, 2825, 2853
BssT1I CCWWGG 3 cut(s) 1926, 2104, 2415
Bst2UI CCWGG 2 cut(s) 555, 1455
Bst6I CTCTTC 6 cut(s) 483, 1404, 1751, 1752, 2328, 2574
BstACI GRCGYC 3 cut(s) 5, 2825, 2853
BstAPI GCANNNNNTGC 1 cut(s) 1108
BstAUI TGTACA 2 cut(s) 1065, 1281
BstBI TTCGAA 1 cut(s) 416
BstC8I GCNNGC 6 cut(s) 925, 2540, 2592, 2696, 2792, 3317
BstDSI CCRYGG 3 cut(s) 344, 1926, 3210
BstENI CCTNNNNNAGG 1 cut(s) 1452
BstF5I GGATG 8 cut(s) 245, 286, 761, 1546, 1870, 2771, 2787, 3073
BstFNI CGCG 4 cut(s) 103, 1751, 2590, 3228
BstHHI GCGC 2 cut(s) 1681, 3230
BstMWI GCNNNNNNNGC 7 cut(s) 731, 1108, 1258, 2470, 2824, 2852, 3325
BstNI CCWGG 2 cut(s) 555, 1455
BstNSI RCATGY 3 cut(s) 1831, 2283, 3058
BstSCI CCNGG 4 cut(s) 553, 971, 1453, 1681
BstSFI CTRYAG 1 cut(s) 909
BstSLI GKGCMC 1 cut(s) 2073
BstUI CGCG 4 cut(s) 103, 1751, 2590, 3228
BstV1I GCAGC 4 cut(s) 895, 2482, 2609, 2889
BstV2I GAAGAC 3 cut(s) 2007, 3039, 3314
BstX2I RGATCY 8 cut(s) 19, 121, 1691, 2079, 2203, 2973, 3170, 3333
BstYI RGATCY 8 cut(s) 19, 121, 1691, 2079, 2203, 2973, 3170, 3333
Bsu36I CCTNAGG 1 cut(s) 2163
BsuI GTATCC 2 cut(s) 1558, 2766
BsuRI GGCC 3 cut(s) 531, 1851, 2246
BtgI CCRYGG 3 cut(s) 344, 1926, 3210
BtrI CACGTC 2 cut(s) 1206, 2256
BtsCI GGATG 8 cut(s) 245, 286, 761, 1546, 1870, 2771, 2787, 3073
BtsI GCAGTG 2 cut(s) 2437, 2727
BtuMI TCGCGA 1 cut(s) 1751
BveI ACCTGC 3 cut(s) 224, 715, 3310
Cac8I GCNNGC 6 cut(s) 925, 2540, 2592, 2696, 2792, 3317
CaiI CAGNNNCTG 1 cut(s) 2439
CfoI GCGC 2 cut(s) 1681, 3230
Cfr13I GGNCC 3 cut(s) 1460, 1850, 2412
CseI GACGC 3 cut(s) 92, 2833, 2861
Csp6I GTAC 5 cut(s) 561, 1066, 1282, 1572, 1645
CviQI GTAC 5 cut(s) 561, 1066, 1282, 1572, 1645
DraI TTTAAA 3 cut(s) 939, 1200, 2704
EaeI YGGCCR 2 cut(s) 529, 2244
Eam1104I CTCTTC 6 cut(s) 483, 1404, 1751, 1752, 2328, 2574
EarI CTCTTC 6 cut(s) 483, 1404, 1751, 1752, 2328, 2574
EciI GGCGGA 1 cut(s) 3256
Eco130I CCWWGG 3 cut(s) 1926, 2104, 2415
Eco31I GGTCTC 2 cut(s) 235, 599
Eco47I GGWCC 2 cut(s) 1460, 2412
Eco57I CTGAAG 8 cut(s) 1097, 1283, 1385, 1677, 2537, 3027, 3326, 3346
Eco81I CCTNAGG 1 cut(s) 2163
Eco88I CYCGRG 1 cut(s) 2152
EcoNI CCTNNNNNAGG 1 cut(s) 1452
EcoO109I RGGNCCY 2 cut(s) 1850, 2412
EcoRI GAATTC 1 cut(s) 3013
EcoRII CCWGG 2 cut(s) 553, 1453
EcoT14I CCWWGG 3 cut(s) 1926, 2104, 2415
EcoT22I ATGCAT 1 cut(s) 761
ErhI CCWWGG 3 cut(s) 1926, 2104, 2415
Esp3I CGTCTC 2 cut(s) 127, 2213
FalI AAGNNNNNCTT 2 cut(s) 1420, 1452
FaqI GGGAC 3 cut(s) 1318, 2238, 2425
FauNDI CATATG 1 cut(s) 640
FbaI TGATCA 2 cut(s) 1213, 2263
FblI GTMKAC 2 cut(s) 2822, 2850
Fnu4HI GCNGC 6 cut(s) 909, 2471, 2595, 2598, 2878, 3226
FokI GGATG 8 cut(s) 232, 293, 768, 1553, 1877, 2778, 2794, 3080
Fsp4HI GCNGC 6 cut(s) 909, 2471, 2595, 2598, 2878, 3226
FspBI CTAG 7 cut(s) 306, 321, 1449, 2216, 2312, 2465, 3044
GlaI GCGC 2 cut(s) 1680, 3229
GluI GCNGC 6 cut(s) 909, 2471, 2595, 2598, 2878, 3226
GsuI CTGGAG 3 cut(s) 1854, 2785, 2935
HaeIII GGCC 3 cut(s) 531, 1851, 2246
HapII CCGG 7 cut(s) 790, 839, 972, 1011, 1682, 2285, 3375
HgaI GACGC 3 cut(s) 92, 2833, 2861
HhaI GCGC 2 cut(s) 1681, 3230
Hin1I GRCGYC 3 cut(s) 5, 2825, 2853
Hin6I GCGC 2 cut(s) 1679, 3228
HinP1I GCGC 2 cut(s) 1679, 3228
HincII GTYRAC 3 cut(s) 2733, 2823, 2851
HindII GTYRAC 3 cut(s) 2733, 2823, 2851
HindIII AAGCTT 2 cut(s) 1265, 2298
HpaII CCGG 7 cut(s) 790, 839, 972, 1011, 1682, 2285, 3375
HphI GGTGA 9 cut(s) 233, 395, 1333, 1835, 1876, 2039, 2299, 2431, 3199
Hpy166II GTNNAC 9 cut(s) 1066, 1324, 1463, 1903, 1976, 2071, 2733, 2823, 2851
Hpy8I GTNNAC 9 cut(s) 1066, 1324, 1463, 1903, 1976, 2071, 2733, 2823, 2851
Hpy99I CGWCG 4 cut(s) 10, 2447, 2827, 2855
HpyCH4IV ACGT 6 cut(s) 136, 559, 1205, 2042, 2255, 2895
HpyF10VI GCNNNNNNNGC 7 cut(s) 731, 1108, 1258, 2470, 2824, 2852, 3325
HpySE526I ACGT 6 cut(s) 136, 559, 1205, 2042, 2255, 2895
Hsp92I GRCGYC 3 cut(s) 5, 2825, 2853
HspAI GCGC 2 cut(s) 1679, 3228
Kpn2I TCCGGA 1 cut(s) 838
Ksp22I TGATCA 2 cut(s) 1213, 2263
LguI GCTCTTC 1 cut(s) 1752
LmnI GCTCC 2 cut(s) 2691, 2954
Lsp1109I GCAGC 4 cut(s) 895, 2482, 2609, 2889
LweI GCATC 5 cut(s) 746, 1111, 1855, 2617, 2673
MaeI CTAG 7 cut(s) 306, 321, 1449, 2216, 2312, 2465, 3044
MaeII ACGT 6 cut(s) 136, 559, 1205, 2042, 2255, 2895
MaeIII GTNAC 8 cut(s) 1071, 1390, 1823, 2143, 2209, 2287, 2435, 3114
MflI RGATCY 8 cut(s) 19, 121, 1691, 2079, 2203, 2973, 3170, 3333
MhlI GDGCHC 2 cut(s) 1045, 2073
MlsI TGGCCA 2 cut(s) 531, 2246
MluNI TGGCCA 2 cut(s) 531, 2246
MlyI GAGTC 6 cut(s) 279, 926, 1613, 1737, 2039, 2151
MmeI TCCRAC 5 cut(s) 239, 1275, 2339, 2357, 2951
Mox20I TGGCCA 2 cut(s) 531, 2246
Mph1103I ATGCAT 1 cut(s) 761
MroI TCCGGA 1 cut(s) 838
MroXI GAANNNNTTC 1 cut(s) 1632
MscI TGGCCA 2 cut(s) 531, 2246
MseI TTAA 7 cut(s) 762, 938, 1199, 2339, 2661, 2703, 2978
MslI CAYNNNNRTG 1 cut(s) 1711
Msp20I TGGCCA 2 cut(s) 531, 2246
MspA1I CMGCKG 1 cut(s) 2557
MspI CCGG 7 cut(s) 790, 839, 972, 1011, 1682, 2285, 3375
MspR9I CCNGG 4 cut(s) 555, 973, 1455, 1683
Mva1269I GAATGC 2 cut(s) 856, 2157
MvaI CCWGG 2 cut(s) 555, 1455
MvnI CGCG 4 cut(s) 103, 1751, 2590, 3228
MwoI GCNNNNNNNGC 7 cut(s) 731, 1108, 1258, 2470, 2824, 2852, 3325
NciI CCSGG 2 cut(s) 973, 1683
NcoI CCATGG 1 cut(s) 1926
NdeI CATATG 1 cut(s) 640
NlaIV GGNNCC 4 cut(s) 1693, 1852, 2413, 3172
NmeAIII GCCGAG 2 cut(s) 880, 1278
NmuCI GTSAC 6 cut(s) 1823, 2143, 2209, 2287, 2435, 3114
NruI TCGCGA 1 cut(s) 1751
NsiI ATGCAT 1 cut(s) 761
NspI RCATGY 3 cut(s) 1831, 2283, 3058
NspV TTCGAA 1 cut(s) 416
PciI ACATGT 3 cut(s) 1827, 2279, 3054
PciSI GCTCTTC 1 cut(s) 1752
PctI GAATGC 2 cut(s) 856, 2157
PdmI GAANNNNTTC 1 cut(s) 1632
PfeI GAWTC 9 cut(s) 632, 864, 1226, 1298, 1355, 1493, 1934, 2350, 2641
Pfl23II CGTACG 1 cut(s) 560
PflFI GACNNNGTC 1 cut(s) 3112
PfoI TCCNGGA 1 cut(s) 553
PkrI GCNGC 6 cut(s) 910, 2472, 2596, 2599, 2879, 3227
PleI GAGTC 6 cut(s) 279, 925, 1613, 1737, 2038, 2150
PpsI GAGTC 6 cut(s) 279, 925, 1613, 1737, 2038, 2150
PpuMI RGGWCCY 1 cut(s) 2412
PscI ACATGT 3 cut(s) 1827, 2279, 3054
PsiI TTATAA 1 cut(s) 968
Psp5II RGGWCCY 1 cut(s) 2412
Psp6I CCWGG 2 cut(s) 553, 1453
PspGI CCWGG 2 cut(s) 553, 1453
PspLI CGTACG 1 cut(s) 560
PspN4I GGNNCC 4 cut(s) 1693, 1852, 2413, 3172
PspPI GGNCC 3 cut(s) 1460, 1850, 2412
PspPPI RGGWCCY 1 cut(s) 2412
PsrI GAACNNNNNNTAC 2 cut(s) 1564, 1596
PstI CTGCAG 1 cut(s) 913
PstNI CAGNNNCTG 1 cut(s) 2439
PsuI RGATCY 8 cut(s) 19, 121, 1691, 2079, 2203, 2973, 3170, 3333
PsyI GACNNNGTC 1 cut(s) 3112
RruI TCGCGA 1 cut(s) 1751
RsaI GTAC 5 cut(s) 562, 1067, 1283, 1573, 1646
RsaNI GTAC 5 cut(s) 561, 1066, 1282, 1572, 1645
RseI CAYNNNNRTG 1 cut(s) 1711
SalI GTCGAC 2 cut(s) 2821, 2849
SapI GCTCTTC 1 cut(s) 1752
SaqAI TTAA 7 cut(s) 762, 938, 1199, 2339, 2661, 2703, 2978
SatI GCNGC 6 cut(s) 909, 2471, 2595, 2598, 2878, 3226
Sau96I GGNCC 3 cut(s) 1460, 1850, 2412
SchI GAGTC 6 cut(s) 279, 926, 1613, 1737, 2039, 2151
ScrFI CCNGG 4 cut(s) 555, 973, 1455, 1683
SduI GDGCHC 2 cut(s) 1045, 2073
SfaNI GCATC 5 cut(s) 746, 1111, 1855, 2617, 2673
SfcI CTRYAG 1 cut(s) 909
SfuI TTCGAA 1 cut(s) 416
SinI GGWCC 2 cut(s) 1460, 2412
SmiMI CAYNNNNRTG 1 cut(s) 1711
SmlI CTYRAG 1 cut(s) 2750
SmoI CTYRAG 1 cut(s) 2750
SsiI CCGC 7 cut(s) 666, 1235, 1458, 2557, 2594, 3226, 3267
SspMI CTAG 7 cut(s) 306, 321, 1449, 2216, 2312, 2465, 3044
StyD4I CCNGG 4 cut(s) 553, 971, 1453, 1681
StyI CCWWGG 3 cut(s) 1926, 2104, 2415
TaiI ACGT 6 cut(s) 139, 562, 1208, 2045, 2258, 2898
TaqI TCGA 9 cut(s) 416, 891, 1171, 1964, 1982, 2022, 2822, 2850, 3021
TatI WGTACW 2 cut(s) 1065, 1281
TauI GCSGC 2 cut(s) 2597, 3228
TfiI GAWTC 9 cut(s) 632, 864, 1226, 1298, 1355, 1493, 1934, 2350, 2641
Tru1I TTAA 7 cut(s) 762, 938, 1199, 2339, 2661, 2703, 2978
Tru9I TTAA 7 cut(s) 762, 938, 1199, 2339, 2661, 2703, 2978
TseFI GTSAC 6 cut(s) 1823, 2143, 2209, 2287, 2435, 3114
TseI GCWGC 4 cut(s) 908, 2470, 2597, 2877
Tsp45I GTSAC 6 cut(s) 1823, 2143, 2209, 2287, 2435, 3114
TspDTI ATGAA 8 cut(s) 624, 732, 810, 939, 1302, 1581, 2161, 2552
TspGWI ACGGA 6 cut(s) 238, 333, 1560, 1897, 2449, 2583
Tth111I GACNNNGTC 1 cut(s) 3112
VneI GTGCAC 1 cut(s) 2069
VpaK11BI GGWCC 2 cut(s) 1460, 2412
XagI CCTNNNNNAGG 1 cut(s) 1452
XapI RAATTY 4 cut(s) 784, 1966, 3013, 3079
XceI RCATGY 3 cut(s) 1831, 2283, 3058
XmiI GTMKAC 2 cut(s) 2822, 2850
XmnI GAANNNNTTC 1 cut(s) 1632
XspI CTAG 7 cut(s) 306, 321, 1449, 2216, 2312, 2465, 3044
Zsp2I ATGCAT 1 cut(s) 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.