Rroxscaffold_1G00002750

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016299
Physical Location & Seq
Forward (+)
3283908 .. 3289736
5829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00002750.1

Sequence Viewer

Length: 3474 bp
ATGGCATCGTCTCTGGAGGACCTTCTTGCAGAAGATGGTTTCAAAGGAAAAAAATTGTGGGCAAGGTCGAGGACTTGCTTGCGGACTGAAACTATGCCTCATCACCATAGTCCAGACCAACCAAAGAGACATTCAGTGTCAGGAGATATAATTAGGACAGGGAGGAGAAGGTCTGATGTAAATCGTAATGGTACAAAAGGTGATGTGCCGACAGGTGATGACATTAGAGGTAGGAGGGGACTAAGAGATGATCTCCTTAGAAGAGATGAAGTTGGTGGAGGATCAAAGAAAGAAGTTAGGAATAGTAAACTTGGAGGACTAGGTTCTACTAGCGGGTGGGAAGCTAGGAGTTTAAATAGCAATTTAGCAGAGAGAGGTAATGCTAGCGTGTGGGAAGCTAGAAGTTTAAAGAGTAATTTATCGGATAGAGGTCATGCCAGTGTGTGGAAGGCTAGAAGCGTGAAGAGTAATTCATCAGACAGAGGTCATGCCAGTGTGTGGGAAGCTGGAAGTTCAAAAAGCAACTTGTCAGAGGATCAGCCGGAAACTGAGATAGTTGAGGTTGAAGATGAAAAATACAAGGACATATATTCAAATGAGTTGTATAGGTCAGAGGGAAGGAACGGTAAATATTCTACTGGAAGTATGGAAAAGGAAGGATTTGATGAGAGGTTAAGGAAGTTGAATGAGGAGGATAGGAGGCACAGCAACAGTTCAACTAACCATGCGTCTGGACGTGTGAGTTTTAGTGAGGTTAACAGGAAAAGCAGGAAACAGCGTGCAAGCTCTCATGACAGATTAAAGAGAGGTTCATCAATCAGCAAAACTTCTGAAGATAGCCGCAGCCGAAAGCGTGATAAAGTTTTACATCCTGCTTCTAAACCTGCTCTTGATGAAATTGCTATCAAGGCCATGGTTTCCATATTAAGTGGATATATTAAGCGTTTTATCAAAGAGGAGGAGTTCCGGATTGCACTTAGAGATAACTGCGTGAGTTCTCTAAACTCTAATGATCAGGAAGAAGAGCATGCTGAGAGCAAAGTCATAGTAAACCTGGAAGAAGCAATGGAAACAATAGCAATGGCTGCTGAGGAGTCTGCAAATGAAAAAGATCTAAGAAGAGCCTCTTTGCAGCTTAGTGTTATCACAGGGTTGACCTCCGATGATTTGAAGGATGGATTTACATCGGGGGTATCGGCAAAGCATCTTCTGCAAGTGTTCTGTGATTCACCATTTACTGCAAGAACGATATTGTTGCCTGAATTGTGGGACTATCTATTTCTTCCACATCTTTCACATTTAAAGGTGTGGTATGATCAGGAGGCTGATTCTCTGGCAGATGCACACAGTGGGCCAAGGAAACTGAAGCTTTTAGGAAAGGTGTACAATGATATTTTAGACTCTGGGACTTACCAATTTGCAGTTTACTACAAGGATTGGCTTACAGAAGGAATTGAATCTCCTAGCATTCCTTCCATCCATATCCCTTCATTATCTCTTCTGCAAGTTCAGCAGGGAGGTTCTCATGGTCATTCTTCAGAGATAGCTAGTCCAGGTGGTCCGCAATCAATGGTCAGCAAAAAACTGTATGATGCAGTGTTTGGCCGCGCAAGTAAAACCGAATTGTATGAAGTTGAAGACGATGGAGAGATTGAAAGCTTTGAAGATTGTATGAGAACTCCTGATGGTTCAGTTGTTGTTGAACAAAAAAGACCGTACTCTGGTGAAGCAACTCAATATGGGTATCGAGACATTGAAGAAGATTCCACTCAGACTGCACTAGAAGATGAATTCCTTGTTGAAAATGGACTCTCAATGGCACCTGAGCAACAATGGAGTTGTTTTGGCGACATTGATCCACCAGAAAGTGACCTCAATGACCAATTTGGTGAAATTTCTAGAGAAAATACAGAAAGCACCAAGATGCTACATCAACCAGCTCCCAAAGAAAAGGAGCTTACTCCAAAAGGGATTACAAAATCAATCTCCACTGTTTCGAATTCTAGTGAAGCATCAATTACCAGTTCTATCATAAATCCAGTCAAAGCAAGTTCTTCTTCTGAAGAGTTGCATGGAAGTTATGTTGAAGGAGGAATTGATTTATCAAGTATTCCCCAAGACTTCCTCTGCCCTCTAAGTGGGGAGTTGTTTGAAGATCCAGTGACTCTAGAGACTGGCCAAACCTTTGAGCGGTCAGCCATCAGGGCATGGTTTGATGAGGGAAACAGAACATGTCCTGTGACAGGAAAAGCTTTGGAATCTCTAGCAGTACCTCTTACAAATTTAATCTTAAAGCGTGTGATTCGTAGTTGGAAGTCTGAACATTCTAGGCAACTCTTAGCTTATGCCTCTCATGTAGTGGGGACCTCAGGGAGAGTAGGATCCAAACACTATGACGAAACAATTATTTTTGTGCTAGAGCAGCTTCTCACTTGTTTCAGCAAAAAGGAAAGAACAGCAAATGCCAGACATCTTATCTCTCTTGGATGCTTGCAGTTTCTTCTTCAACGGTTTGAAGTAGGAAAGGAGGAAGAGAAATCACGTGTAGCAGCACTCTTTGCCTGTTGTATAAAAGCAGATACAGATTGTAGGAATCTAATTGCAAGCAGCATCAACAAACAGTGCCTTGTGGAGCTGCTTCAGGGAAAAGAAGTTAACATGAGAACAAATGCTGTGTTGTTGATGACAGAACTCATTTGCTTGAAGAGGTGGGATATACCGACTTTTGGGAAGAAAGATGTTTCATTATTTCTCACTGGCTTGCAGAATGAAGGGATGGTGAATACAATGGCCATTTTGCATGAGCATATCCAGAGTTCTTTACCCAACCAAAGGCCCCTGGCTGCTGTGCTTTTGTTCTACCTAGATATTTTGGTATCAATTGAACCTCAAGAGTATAGCATACATAGATCGGAAGCAGTTGATGCCCTTGCCGAATGTCTTGATTGCAGCTTAACTGATCTGAATGTCCGAGAGAATTGCTGCAAAGCACTTCTTATCTTGGGAATGCATTTCTCCATCTCTGGAAAATTGTTGTCAGAAAAGTGGATAACAGAGGAAGCAAATCCTAATGGTAATGGTGAAGTGAATTCGGTTGACAATGAAGACGGTTCACTAGCTTCTGACAGATATCCATCGGATGATGAAGAAAATTTGACTGAATATTGGTTGAGGATGTGCCTAATCACTGCAGAATGGTTGAGCCGAGCACTTTCTTCACTATCTGATTCCGAGTTTCAGCTCTTAGCCTTCTCATCTCTCATTCCTCCACTGAAGGAAAACCTGAAGAACAGTGAGCACGTTGAGCACAAAATTCTTGCTTCAATGTCTCTGCTCAACTTCAGCAAAATCTCTGAGTGCAGGGTGCTTTTGATGGATAGCACAGAAGATATTTCAATTCCTCTACAAAACCTTGCTGAAGTAACATGGAGTGCGAAAGTGTTATATGCCATCATTTCTGGAGACAATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
U-box

Protein Analysis

1157

Amino Acids

128.9

Weight (kDa)

5.36

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_LIN PF23568 301 - 399 3.5e-26 E3 ubiquitin-protein ligase LIN-like, ARM repeat
ARM_LIN PF23568 398 - 497 1.1e-47 E3 ubiquitin-protein ligase LIN-like, ARM repeat
U-box PF04564 704 - 776 6.5e-14 U-box domain
ARM_LIN_C PF23628 808 - 1058 6.7e-81 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
ARM_LIN_C PF23628 1058 - 1153 1.5e-33 Putative E3 ubiquitin-protein ligase LIN, ARM-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016299)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g44280
malus_domestica MD11G1016300.v1.1
prunus_persica Prupe.6G014500_v2.0.a1
pyrus_communis pycom11g01150
rosa_chinensis RchiOBHm_Chr5g0080651
rosa_laevigata RLG00000036879
rosa_multiflora Rmu_sc0002380.1_g000007 Rmu_sc0039404.1_g000001
rosa_roxburghii Rroxscaffold_1G00002750
rosa_rugosa Rorug05G0470900
rosa_samantha Rh5AG524200 Rh5BG546500 Rh5CG569700 Rh5DG556700
rosa_wichuraiana Rw5G048630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 892
AccB1I GGYRCC 1 cut(s) 1819
AccB7I CCANNNNNTGG 2 cut(s) 444, 498
AccBSI CCGCTC 1 cut(s) 2191
AccII CGCG 1 cut(s) 1608
AccIII TCCGGA 1 cut(s) 966
AciI CCGC 6 cut(s) 82, 333, 841, 1562, 1606, 2191
AclWI GGATC 6 cut(s) 289, 543, 1850, 2150, 2376, 2389
AcoI YGGCCR 3 cut(s) 1603, 2176, 2790
AcsI RAATTY 7 cut(s) 1790, 1893, 1999, 2281, 3088, 3151, 3312
AcuI CTGAAG 9 cut(s) 852, 1385, 1521, 2082, 2624, 3293, 3305, 3325, 3438
AcvI CACGTG 1 cut(s) 2543
AdeI CACNNNGTG 1 cut(s) 1349
AfaI GTAC 4 cut(s) 193, 1385, 1718, 2271
AfiI CCNNNNNNNGG 8 cut(s) 444, 498, 1721, 2138, 2190, 2243, 2726, 2832
AflIII ACRYGT 3 cut(s) 736, 2231, 2542
AjiI CACGTC 1 cut(s) 737
AjnI CCWGG 3 cut(s) 1053, 1552, 2838
AjuI GAANNNNNNNTTGG 2 cut(s) 115, 147
Alw21I GWGCWC 3 cut(s) 3211, 3300, 3309
Alw26I GTCTC 6 cut(s) 15, 121, 1743, 2165, 3333, 3456
AlwI GGATC 6 cut(s) 289, 543, 1850, 2150, 2376, 2389
Aor13HI TCCGGA 1 cut(s) 966
AoxI GGCC 6 cut(s) 909, 1352, 1603, 2176, 2790, 2834
ApoI RAATTY 7 cut(s) 1790, 1893, 1999, 2281, 3088, 3151, 3312
AspLEI GCGC 1 cut(s) 1610
AspS9I GGNCC 5 cut(s) 19, 1352, 1559, 2364, 2835
AsuHPI GGTGA 8 cut(s) 95, 212, 227, 1221, 1736, 1901, 2791, 3092
AsuII TTCGAA 1 cut(s) 1997
AsuNHI GCTAGC 1 cut(s) 383
AvaII GGWCC 3 cut(s) 19, 1559, 2364
AxyI CCTNAGG 1 cut(s) 2368
BalI TGGCCA 2 cut(s) 2178, 2792
BamHI GGATCC 1 cut(s) 2381
BanI GGYRCC 1 cut(s) 1819
BbrPI CACGTG 1 cut(s) 2543
BbsI GAAGAC 2 cut(s) 1644, 3111
Bbv12I GWGCWC 3 cut(s) 3211, 3300, 3309
BbvCI CCTCAGC 1 cut(s) 1089
BcgI CGANNNNNNTGC 4 cut(s) 1237, 1271, 2961, 2995
BciT130I CCWGG 3 cut(s) 1055, 1554, 2840
BclI TGATCA 2 cut(s) 1012, 1315
BcoDI GTCTC 6 cut(s) 15, 121, 1743, 2165, 3333, 3456
BfmI CTRYAG 1 cut(s) 3189
BfuAI ACCTGC 1 cut(s) 892
BglI GCCNNNNNGGC 1 cut(s) 2204
BglII AGATCT 1 cut(s) 1111
Bme1390I CCNGG 3 cut(s) 1055, 1554, 2840
Bme18I GGWCC 3 cut(s) 19, 1559, 2364
BmgBI CACGTC 1 cut(s) 737
BmgT120I GGNCC 5 cut(s) 19, 1352, 1559, 2364, 2835
BmiI GGNNCC 4 cut(s) 1821, 2365, 2383, 2837
BmrFI CCNGG 3 cut(s) 1055, 1554, 2840
BmsI GCATC 9 cut(s) 14, 1213, 1330, 1582, 1914, 2021, 2477, 2619, 2914
BmtI GCTAGC 1 cut(s) 387
BoxI GACNNNNGTC 1 cut(s) 483
BpiI GAAGAC 2 cut(s) 1644, 3111
BplI GAGNNNNNCTC 2 cut(s) 237, 269
BpmI CTGGAG 1 cut(s) 35
Bpu10I CCTNAGC 2 cut(s) 1089, 1824
Bpu14I TTCGAA 1 cut(s) 1997
BpuEI CTTGAG 1 cut(s) 2874
BsaAI YACGTR 1 cut(s) 2543
BsaBI GATNNNNATC 3 cut(s) 180, 1183, 3133
BsaJI CCNNGG 3 cut(s) 912, 1355, 2838
BsaWI WCCGGW 1 cut(s) 966
Bsc4I CCNNNNNNNGG 8 cut(s) 444, 498, 1721, 2138, 2190, 2243, 2726, 2832
Bse1I ACTGG 8 cut(s) 438, 492, 643, 2022, 2039, 2159, 2179, 2761
Bse21I CCTNAGG 1 cut(s) 2368
Bse3DI GCAATG 2 cut(s) 1071, 1086
Bse8I GATNNNNATC 3 cut(s) 180, 1183, 3133
BseAI TCCGGA 1 cut(s) 966
BseBI CCWGG 3 cut(s) 1055, 1554, 2840
BseDI CCNNGG 3 cut(s) 912, 1355, 2838
BseGI GGATG 7 cut(s) 868, 1180, 1476, 2492, 2781, 3145, 3180
BseJI GATNNNNATC 3 cut(s) 180, 1183, 3133
BseLI CCNNNNNNNGG 8 cut(s) 444, 498, 1721, 2138, 2190, 2243, 2726, 2832
BseMI GCAATG 2 cut(s) 1071, 1086
BseMII CTCAG 7 cut(s) 540, 1023, 1080, 1784, 1815, 2382, 3345
BseNI ACTGG 8 cut(s) 438, 492, 643, 2022, 2039, 2159, 2179, 2761
BseRI GAGGAG 5 cut(s) 178, 704, 971, 974, 1106
BsgI GTGCAG 2 cut(s) 1761, 3379
Bsh1236I CGCG 1 cut(s) 1608
BshFI GGCC 6 cut(s) 911, 1354, 1605, 2178, 2792, 2836
BshNI GGYRCC 1 cut(s) 1819
BsiHKAI GWGCWC 3 cut(s) 3211, 3300, 3309
BsiSI CCGG 2 cut(s) 542, 967
BslFI GGGAC 4 cut(s) 252, 1283, 1420, 2377
BslI CCNNNNNNNGG 8 cut(s) 444, 498, 1721, 2138, 2190, 2243, 2726, 2832
BsmAI GTCTC 6 cut(s) 15, 121, 1743, 2165, 3333, 3456
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 4 cut(s) 252, 1283, 1420, 2377
BsmI GAATGC 2 cut(s) 1467, 3012
BsnI GGCC 6 cut(s) 911, 1354, 1605, 2178, 2792, 2836
Bsp119I TTCGAA 1 cut(s) 1997
Bsp1286I GDGCHC 3 cut(s) 3211, 3300, 3309
Bsp13I TCCGGA 1 cut(s) 966
Bsp1407I TGTACA 1 cut(s) 1383
Bsp19I CCATGG 1 cut(s) 912
BspACI CCGC 6 cut(s) 82, 333, 841, 1562, 1606, 2191
BspANI GGCC 6 cut(s) 911, 1354, 1605, 2178, 2792, 2836
BspCNI CTCAG 7 cut(s) 541, 1024, 1081, 1783, 1816, 2381, 3346
BspEI TCCGGA 1 cut(s) 966
BspFNI CGCG 1 cut(s) 1608
BspHI TCATGA 1 cut(s) 790
BspLI GGNNCC 4 cut(s) 1821, 2365, 2383, 2837
BspMAI CTGCAG 1 cut(s) 3193
BspMI ACCTGC 1 cut(s) 892
BspOI GCTAGC 1 cut(s) 387
BspPI GGATC 6 cut(s) 289, 543, 1850, 2150, 2376, 2389
BspQI GCTCTTC 2 cut(s) 1017, 1114
BspT104I TTCGAA 1 cut(s) 1997
BspT107I GGYRCC 1 cut(s) 1819
BsrBI CCGCTC 1 cut(s) 2191
BsrDI GCAATG 2 cut(s) 1071, 1086
BsrGI TGTACA 1 cut(s) 1383
BsrI ACTGG 8 cut(s) 438, 492, 643, 2022, 2039, 2159, 2179, 2761
BssECI CCNNGG 3 cut(s) 912, 1355, 2838
BssT1I CCWWGG 2 cut(s) 912, 1355
Bst2UI CCWGG 3 cut(s) 1055, 1554, 2840
Bst6I CTCTTC 8 cut(s) 256, 458, 1017, 1114, 1503, 2058, 2526, 2699
BstAPI GCANNNNNTGC 4 cut(s) 1085, 1210, 2558, 2924
BstAUI TGTACA 1 cut(s) 1383
BstBAI YACGTR 1 cut(s) 2543
BstBI TTCGAA 1 cut(s) 1997
BstC8I GCNNGC 8 cut(s) 80, 385, 780, 784, 1029, 2492, 2605, 2762
BstDSI CCRYGG 1 cut(s) 912
BstENI CCTNNNNNAGG 1 cut(s) 2241
BstF5I GGATG 7 cut(s) 868, 1180, 1476, 2492, 2781, 3145, 3180
BstFNI CGCG 1 cut(s) 1608
BstHHI GCGC 1 cut(s) 1610
BstMAI GTCTC 6 cut(s) 15, 121, 1743, 2165, 3333, 3456
BstMWI GCNNNNNNNGC 9 cut(s) 908, 1085, 1210, 1510, 2204, 2422, 2558, 2924, 3304
BstNI CCWGG 3 cut(s) 1055, 1554, 2840
BstNSI RCATGY 2 cut(s) 1031, 2235
BstPAI GACNNNNGTC 1 cut(s) 483
BstSCI CCNGG 3 cut(s) 1053, 1552, 2838
BstSFI CTRYAG 1 cut(s) 3189
BstUI CGCG 1 cut(s) 1608
BstV2I GAAGAC 2 cut(s) 1644, 3111
BstX2I RGATCY 3 cut(s) 1111, 2155, 2381
BstXI CCANNNNNNTGG 1 cut(s) 731
BstYI RGATCY 3 cut(s) 1111, 2155, 2381
Bsu36I CCTNAGG 1 cut(s) 2368
BsuRI GGCC 6 cut(s) 911, 1354, 1605, 2178, 2792, 2836
BtgI CCRYGG 1 cut(s) 912
BtrI CACGTC 1 cut(s) 737
BtsCI GGATG 7 cut(s) 868, 1180, 1476, 2492, 2781, 3145, 3180
BtsI GCAGTG 2 cut(s) 1602, 3186
BveI ACCTGC 1 cut(s) 892
Cac8I GCNNGC 8 cut(s) 80, 385, 780, 784, 1029, 2492, 2605, 2762
CciI TCATGA 1 cut(s) 790
CfoI GCGC 1 cut(s) 1610
Cfr13I GGNCC 5 cut(s) 19, 1352, 1559, 2364, 2835
CseI GACGC 1 cut(s) 717
Csp6I GTAC 4 cut(s) 192, 1384, 1717, 2270
CviQI GTAC 4 cut(s) 192, 1384, 1717, 2270
DraI TTTAAA 3 cut(s) 354, 408, 1302
DraIII CACNNNGTG 1 cut(s) 1349
EaeI YGGCCR 3 cut(s) 1603, 2176, 2790
Eam1104I CTCTTC 8 cut(s) 256, 458, 1017, 1114, 1503, 2058, 2526, 2699
EarI CTCTTC 8 cut(s) 256, 458, 1017, 1114, 1503, 2058, 2526, 2699
Eco130I CCWWGG 2 cut(s) 912, 1355
Eco32I GATATC 1 cut(s) 3131
Eco47I GGWCC 3 cut(s) 19, 1559, 2364
Eco57I CTGAAG 9 cut(s) 852, 1385, 1521, 2082, 2624, 3293, 3305, 3325, 3438
Eco72I CACGTG 1 cut(s) 2543
Eco81I CCTNAGG 1 cut(s) 2368
EcoNI CCTNNNNNAGG 1 cut(s) 2241
EcoO109I RGGNCCY 3 cut(s) 19, 2364, 2835
EcoRI GAATTC 3 cut(s) 1790, 1999, 3088
EcoRII CCWGG 3 cut(s) 1053, 1552, 2838
EcoRV GATATC 1 cut(s) 3131
EcoT14I CCWWGG 2 cut(s) 912, 1355
EcoT22I ATGCAT 1 cut(s) 3012
ErhI CCWWGG 2 cut(s) 912, 1355
Esp3I CGTCTC 1 cut(s) 15
FalI AAGNNNNNCTT 6 cut(s) 1111, 1143, 2041, 2073, 2979, 3011
FaqI GGGAC 4 cut(s) 252, 1283, 1420, 2377
FauI CCCGC 1 cut(s) 326
FbaI TGATCA 2 cut(s) 1012, 1315
FokI GGATG 7 cut(s) 855, 1187, 1463, 2499, 2788, 3152, 3187
GlaI GCGC 1 cut(s) 1609
GsuI CTGGAG 1 cut(s) 35
HaeIII GGCC 6 cut(s) 911, 1354, 1605, 2178, 2792, 2836
HapII CCGG 2 cut(s) 542, 967
HgaI GACGC 1 cut(s) 717
HhaI GCGC 1 cut(s) 1610
Hin6I GCGC 1 cut(s) 1608
HinP1I GCGC 1 cut(s) 1608
HincII GTYRAC 4 cut(s) 757, 1155, 2656, 3097
HindII GTYRAC 4 cut(s) 757, 1155, 2656, 3097
HindIII AAGCTT 3 cut(s) 1367, 1657, 2250
HpaI GTTAAC 2 cut(s) 757, 2656
HpaII CCGG 2 cut(s) 542, 967
HphI GGTGA 8 cut(s) 95, 212, 227, 1221, 1736, 1901, 2791, 3092
Hpy166II GTNNAC 9 cut(s) 308, 757, 1051, 1155, 1384, 1426, 2656, 3097, 3113
Hpy8I GTNNAC 9 cut(s) 308, 757, 1051, 1155, 1384, 1426, 2656, 3097, 3113
HpyCH4IV ACGT 3 cut(s) 736, 2542, 3300
HpyF10VI GCNNNNNNNGC 9 cut(s) 908, 1085, 1210, 1510, 2204, 2422, 2558, 2924, 3304
HpySE526I ACGT 3 cut(s) 736, 2542, 3300
HspAI GCGC 1 cut(s) 1608
Kpn2I TCCGGA 1 cut(s) 966
Ksp22I TGATCA 2 cut(s) 1012, 1315
KspAI GTTAAC 2 cut(s) 757, 2656
LguI GCTCTTC 2 cut(s) 1017, 1114
LmnI GCTCC 3 cut(s) 1945, 1954, 2632
LweI GCATC 9 cut(s) 14, 1213, 1330, 1582, 1914, 2021, 2477, 2619, 2914
MaeII ACGT 3 cut(s) 736, 2542, 3300
MaeIII GTNAC 4 cut(s) 1868, 2161, 2239, 3421
MbiI CCGCTC 1 cut(s) 2191
MfeI CAATTG 1 cut(s) 2880
MflI RGATCY 3 cut(s) 1111, 2155, 2381
MhlI GDGCHC 3 cut(s) 3211, 3300, 3309
MlsI TGGCCA 2 cut(s) 2178, 2792
MluNI TGGCCA 2 cut(s) 2178, 2792
MlyI GAGTC 4 cut(s) 1103, 1394, 1803, 2158
MmeI TCCRAC 1 cut(s) 2291
Mox20I TGGCCA 2 cut(s) 2178, 2792
Mph1103I ATGCAT 1 cut(s) 3012
MroI TCCGGA 1 cut(s) 966
MscI TGGCCA 2 cut(s) 2178, 2792
MslI CAYNNNNRTG 3 cut(s) 438, 492, 1922
Msp20I TGGCCA 2 cut(s) 2178, 2792
MspI CCGG 2 cut(s) 542, 967
MspR9I CCNGG 3 cut(s) 1055, 1554, 2840
MunI CAATTG 1 cut(s) 2880
Mva1269I GAATGC 2 cut(s) 1467, 3012
MvaI CCWGG 3 cut(s) 1055, 1554, 2840
MvnI CGCG 1 cut(s) 1608
MwoI GCNNNNNNNGC 9 cut(s) 908, 1085, 1210, 1510, 2204, 2422, 2558, 2924, 3304
NcoI CCATGG 1 cut(s) 912
NheI GCTAGC 1 cut(s) 383
NlaIV GGNNCC 4 cut(s) 1821, 2365, 2383, 2837
NmeAIII GCCGAG 1 cut(s) 3230
NmuCI GTSAC 3 cut(s) 1868, 2161, 2239
NsiI ATGCAT 1 cut(s) 3012
NspI RCATGY 2 cut(s) 1031, 2235
NspV TTCGAA 1 cut(s) 1997
PaeI GCATGC 1 cut(s) 1031
PagI TCATGA 1 cut(s) 790
PciI ACATGT 1 cut(s) 2231
PciSI GCTCTTC 2 cut(s) 1017, 1114
PctI GAATGC 2 cut(s) 1467, 3012
PfeI GAWTC 8 cut(s) 1226, 1328, 1457, 1763, 2258, 2302, 2593, 3227
PflMI CCANNNNNTGG 2 cut(s) 444, 498
PleI GAGTC 4 cut(s) 1102, 1394, 1803, 2158
PmaCI CACGTG 1 cut(s) 2543
PmlI CACGTG 1 cut(s) 2543
PpsI GAGTC 4 cut(s) 1102, 1394, 1803, 2158
Ppu21I YACGTR 1 cut(s) 2543
PpuMI RGGWCCY 2 cut(s) 19, 2364
PscI ACATGT 1 cut(s) 2231
PshAI GACNNNNGTC 1 cut(s) 483
Psp5II RGGWCCY 2 cut(s) 19, 2364
Psp6I CCWGG 3 cut(s) 1053, 1552, 2838
PspCI CACGTG 1 cut(s) 2543
PspGI CCWGG 3 cut(s) 1053, 1552, 2838
PspN4I GGNNCC 4 cut(s) 1821, 2365, 2383, 2837
PspPI GGNCC 5 cut(s) 19, 1352, 1559, 2364, 2835
PspPPI RGGWCCY 2 cut(s) 19, 2364
PstI CTGCAG 1 cut(s) 3193
PsuI RGATCY 3 cut(s) 1111, 2155, 2381
RsaI GTAC 4 cut(s) 193, 1385, 1718, 2271
RsaNI GTAC 4 cut(s) 192, 1384, 1717, 2270
RseI CAYNNNNRTG 3 cut(s) 438, 492, 1922
SapI GCTCTTC 2 cut(s) 1017, 1114
Sau96I GGNCC 5 cut(s) 19, 1352, 1559, 2364, 2835
SchI GAGTC 4 cut(s) 1103, 1394, 1803, 2158
ScrFI CCNGG 3 cut(s) 1055, 1554, 2840
SduI GDGCHC 3 cut(s) 3211, 3300, 3309
SfaNI GCATC 9 cut(s) 14, 1213, 1330, 1582, 1914, 2021, 2477, 2619, 2914
SfcI CTRYAG 1 cut(s) 3189
SfuI TTCGAA 1 cut(s) 1997
SinI GGWCC 3 cut(s) 19, 1559, 2364
SmiMI CAYNNNNRTG 3 cut(s) 438, 492, 1922
SmlI CTYRAG 1 cut(s) 2889
SmoI CTYRAG 1 cut(s) 2889
SphI GCATGC 1 cut(s) 1031
SsiI CCGC 6 cut(s) 82, 333, 841, 1562, 1606, 2191
SspI AATATT 2 cut(s) 632, 3164
StyD4I CCNGG 3 cut(s) 1053, 1552, 2838
StyI CCWWGG 2 cut(s) 912, 1355
TaiI ACGT 3 cut(s) 739, 2545, 3303
TaqI TCGA 3 cut(s) 68, 1747, 1997
TatI WGTACW 1 cut(s) 1383
TauI GCSGC 2 cut(s) 843, 1608
TfiI GAWTC 8 cut(s) 1226, 1328, 1457, 1763, 2258, 2302, 2593, 3227
TseFI GTSAC 3 cut(s) 1868, 2161, 2239
Tsp45I GTSAC 3 cut(s) 1868, 2161, 2239
Van91I CCANNNNNTGG 2 cut(s) 444, 498
VpaK11BI GGWCC 3 cut(s) 19, 1559, 2364
XagI CCTNNNNNAGG 1 cut(s) 2241
XapI RAATTY 7 cut(s) 1790, 1893, 1999, 2281, 3088, 3151, 3312
XbaI TCTAGA 2 cut(s) 1898, 2167
XceI RCATGY 2 cut(s) 1031, 2235
Zsp2I ATGCAT 1 cut(s) 3012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.