pycom11g02070

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007439
Physical Location & Seq
Reverse (-)
1776109 .. 1782611
6503 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1623 bp
ATGCATGCAGGTGTGGATAACTTGGAAACGGACATGGATAAACAAAAGGCGACGGTGACGGGGTATGTAGATCAAAGGAAGGTGTTAAAGGTTGTGGGAGAAGAACAGGGAGGAGGGCCGGATTTTGGCTGTTCCCATACGACGGCGAATACTATCCAAAACCCCAAATTCAAACCTCTCAATTCGCACACTGACACTTTCATGGAAAAATACTTGGTCAGTCTCAAGCCCTCGTCTCAAAATCCCAGCAAACCTATCTCACGGTGCAGATGGAGTCGGAGCATGATCGAACTGAACGGAAGATTCGAGCCCAATTATCGCCATTCTGTCTCCGGTTTACTCATGGAATCATATTCGGAGCTTAAGAAATTTCCGCATTTGTACCACTTCAATGGCGGACCGTGTCAAACCCATTTGAATTGGATCGACAATGGAGCGTTTCTTTATCACCAAGCGCCAATCACAAAGCAAGGAATCTCTGCACTGGATTTTGACCAAAAGGGAATATACCTGGCATCTGTGACAAAATCAGGGTGCTTAACAGTGCATGATTTCGAAACTCTTTATTGCCAGAGTAATGAACCTTTGCACCGCTTGAAGGAAGATGAATCAAAACATGTGGTGCACCTTTCTTTCGATCAACAACTAGACTTTGTTCGGTGGAATCTTGCTAATCAAGATGAGGTTGTTTGCACATCTACAAAAAGGAATGAAGTACTTATATTTGATATTAGTTATATCTCATCTAAACCATCCCAGGTGTTAAGGACAAGACATAGTGTTTCTGTCTTTGGGTCTAATATTCATAAAGGCCTTACTGATATTGCTTCTACTTCACTTGACGATTCCAGAATACTGGCTTCTGATACACATGGTGCAATCAACGTATGGGACAGAAGAAAGAGCACATTTCCATGTCTTGAACTCACTACAGATTCCCGTGGAACCCTTAATAGTATACAATTGAATGTGGAAAATCAGATCGTTTTTGGAGCTGGAAAGAATGGATTTATTTATATATGGGATCTTCGTGGTGGAAGAACATCTTCTACTCTTCAGAGTAATAAAGAAATACGTCATCCACCTCTAACATCATTGAAGTTATCATCAATGTTAGAGAAAATTGGGCCTCTGAAGGCACAATCAGACATTATTTCTAAGGAGATACAATCCATAAATCTCGATCCATCTTGCCCGCATCAGTTGGCATTCCACCTTGATGATGGTTGGTCAGGTGTTTTGGATGTTTATAATTTTCAAGTTACACATATTCATTGTCCTCCGCCTGCTTGGTTAAATGATTCTGGTGTATCAGCTGATCTGTTATATTTGAGAAAACCTTCATGGCTGCCAATAAATTCGATCTATGTGGTTGGATCATCAACTGACAGTGGGATTTATATTTTGGATTTCTATCCCGATTCAAGCTCTCCTAGCCATGTGGACTACATAGACGATGCACAAAATCTTTCTGGGGTAAAGAATCAAGAGAAGAAAAACAGGTTTCTTCCATTGTCAGAAAGGGTTACAGCATGTGCAGCTCATCCTCTCAACAGTACCATCATTGCTGGAACAAGGAATTCATCTTTGCTCGTGATTTCGCAAAGTCACAAGTCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

541

Amino Acids

60.34

Weight (kDa)

6.76

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 156 - 367 2.1e-08 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1251
AccI GTMKAC 1 cut(s) 958
AciI CCGC 5 cut(s) 374, 396, 592, 1196, 1283
AclWI GGATC 4 cut(s) 431, 1032, 1178, 1384
AcsI RAATTY 4 cut(s) 167, 368, 1357, 1579
AcuI CTGAAG 2 cut(s) 1040, 1154
AdeI CACNNNGTG 1 cut(s) 875
AfaI GTAC 3 cut(s) 383, 717, 1558
AfiI CCNNNNNNNGG 3 cut(s) 125, 142, 598
AflII CTTAAG 1 cut(s) 362
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 9 cut(s) 172, 391, 418, 598, 923, 967, 1099, 1259, 1425
AjnI CCWGG 2 cut(s) 510, 756
AluBI AGCT 5 cut(s) 361, 995, 1316, 1428, 1541
AluI AGCT 5 cut(s) 361, 995, 1316, 1428, 1541
Alw21I GWGCWC 2 cut(s) 627, 908
Alw26I GTCTC 3 cut(s) 227, 240, 334
Alw44I GTGCAC 1 cut(s) 623
AlwI GGATC 4 cut(s) 431, 1032, 1178, 1384
AoxI GGCC 3 cut(s) 116, 811, 1127
ApaLI GTGCAC 1 cut(s) 623
ApeKI GCWGC 2 cut(s) 1348, 1538
ApoI RAATTY 4 cut(s) 167, 368, 1357, 1579
Asp700I GAANNNNTTC 2 cut(s) 1045, 1339
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 3 cut(s) 116, 398, 1127
AsuHPI GGTGA 2 cut(s) 67, 440
AsuII TTCGAA 1 cut(s) 555
AvaII GGWCC 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 627
BanII GRGCYC 1 cut(s) 312
BauI CACGAG 1 cut(s) 1592
Bbv12I GWGCWC 2 cut(s) 627, 908
BbvI GCAGC 2 cut(s) 1335, 1550
BccI CCATC 5 cut(s) 264, 760, 1195, 1217, 1568
BceAI ACGGC 1 cut(s) 159
BciT130I CCWGG 2 cut(s) 512, 758
BcoDI GTCTC 3 cut(s) 227, 240, 334
BfaI CTAG 2 cut(s) 647, 1434
BfmI CTRYAG 1 cut(s) 930
BfoI RGCGCY 1 cut(s) 458
BfrI CTTAAG 1 cut(s) 362
BisI GCNGC 2 cut(s) 1349, 1539
BlsI GCNGC 2 cut(s) 1350, 1540
BmcAI AGTACT 1 cut(s) 717
Bme1390I CCNGG 2 cut(s) 512, 758
Bme18I GGWCC 1 cut(s) 398
BmgT120I GGNCC 3 cut(s) 116, 398, 1127
BmiI GGNNCC 1 cut(s) 946
BmrFI CCNGG 2 cut(s) 512, 758
BmsI GCATC 3 cut(s) 524, 1207, 1447
Bpu14I TTCGAA 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 209
BsaBI GATNNNNATC 1 cut(s) 1413
BsaJI CCNNGG 2 cut(s) 756, 940
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 142, 598
Bse1I ACTGG 2 cut(s) 489, 861
Bse3DI GCAATG 1 cut(s) 1563
Bse8I GATNNNNATC 1 cut(s) 1413
BseBI CCWGG 2 cut(s) 512, 758
BseDI CCNNGG 2 cut(s) 756, 940
BseGI GGATG 4 cut(s) 752, 1078, 1249, 1543
BseJI GATNNNNATC 1 cut(s) 1413
BseLI CCNNNNNNNGG 3 cut(s) 125, 142, 598
BseMI GCAATG 1 cut(s) 1563
BseNI ACTGG 2 cut(s) 489, 861
BseRI GAGGAG 1 cut(s) 126
BseSI GKGCMC 1 cut(s) 627
BseXI GCAGC 2 cut(s) 1335, 1550
BseYI CCCAGC 1 cut(s) 245
BsgI GTGCAG 3 cut(s) 286, 465, 1557
BshFI GGCC 3 cut(s) 118, 813, 1129
BsiHKAI GWGCWC 2 cut(s) 627, 908
BsiSI CCGG 2 cut(s) 119, 333
BslFI GGGAC 1 cut(s) 905
BslI CCNNNNNNNGG 3 cut(s) 125, 142, 598
BsmAI GTCTC 3 cut(s) 227, 240, 334
BsmBI CGTCTC 1 cut(s) 240
BsmFI GGGAC 1 cut(s) 905
BsmI GAATGC 1 cut(s) 1208
BsnI GGCC 3 cut(s) 118, 813, 1129
Bsp119I TTCGAA 1 cut(s) 555
Bsp1286I GDGCHC 3 cut(s) 312, 627, 908
BspACI CCGC 5 cut(s) 374, 396, 592, 1196, 1283
BspANI GGCC 3 cut(s) 118, 813, 1129
BspLI GGNNCC 1 cut(s) 946
BspPI GGATC 4 cut(s) 431, 1032, 1178, 1384
BspT104I TTCGAA 1 cut(s) 555
BspTI CTTAAG 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 1563
BsrI ACTGG 2 cut(s) 489, 861
BssECI CCNNGG 2 cut(s) 756, 940
BssNAI GTATAC 1 cut(s) 959
BssSI CACGAG 1 cut(s) 1592
Bst1107I GTATAC 1 cut(s) 959
Bst2BI CACGAG 1 cut(s) 1592
Bst2UI CCWGG 2 cut(s) 512, 758
Bst4CI ACNGT 6 cut(s) 55, 264, 402, 544, 1391, 1556
Bst6I CTCTTC 1 cut(s) 1059
BstAFI CTTAAG 1 cut(s) 362
BstBI TTCGAA 1 cut(s) 555
BstC8I GCNNGC 3 cut(s) 6, 1196, 1287
BstDEI CTNAG 1 cut(s) 1158
BstDSI CCRYGG 1 cut(s) 940
BstF5I GGATG 4 cut(s) 752, 1078, 1249, 1543
BstH2I RGCGCY 1 cut(s) 458
BstHHI GCGC 1 cut(s) 457
BstMAI GTCTC 3 cut(s) 227, 240, 334
BstMWI GCNNNNNNNGC 2 cut(s) 1434, 1538
BstNI CCWGG 2 cut(s) 512, 758
BstNSI RCATGY 3 cut(s) 8, 620, 1536
BstSCI CCNGG 2 cut(s) 510, 756
BstSFI CTRYAG 1 cut(s) 930
BstSLI GKGCMC 1 cut(s) 627
BstV1I GCAGC 2 cut(s) 1335, 1550
BstX2I RGATCY 1 cut(s) 1024
BstXI CCANNNNNNTGG 2 cut(s) 392, 856
BstYI RGATCY 1 cut(s) 1024
BstZ17I GTATAC 1 cut(s) 959
BsuRI GGCC 3 cut(s) 118, 813, 1129
BtgI CCRYGG 1 cut(s) 940
BtsCI GGATG 4 cut(s) 752, 1078, 1249, 1543
BtsIMutI CAGTG 4 cut(s) 189, 482, 549, 1396
Cac8I GCNNGC 3 cut(s) 6, 1196, 1287
CfoI GCGC 1 cut(s) 457
Cfr13I GGNCC 3 cut(s) 116, 398, 1127
CpoI CGGWCCG 1 cut(s) 398
Csp6I GTAC 3 cut(s) 382, 716, 1557
CspCI CAANNNNNGTGG 2 cut(s) 600, 635
CspI CGGWCCG 1 cut(s) 398
CviQI GTAC 3 cut(s) 382, 716, 1557
DdeI CTNAG 1 cut(s) 1158
DraIII CACNNNGTG 1 cut(s) 875
Eam1104I CTCTTC 1 cut(s) 1059
EarI CTCTTC 1 cut(s) 1059
EciI GGCGGA 2 cut(s) 411, 1272
Eco147I AGGCCT 1 cut(s) 813
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 398
Eco57I CTGAAG 2 cut(s) 1040, 1154
EcoRI GAATTC 1 cut(s) 1579
EcoRII CCWGG 2 cut(s) 510, 756
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 312
Esp3I CGTCTC 1 cut(s) 240
FalI AAGNNNNNCTT 2 cut(s) 1030, 1062
FaqI GGGAC 1 cut(s) 905
FauI CCCGC 1 cut(s) 1203
FblI GTMKAC 1 cut(s) 958
Fnu4HI GCNGC 2 cut(s) 1349, 1539
FokI GGATG 4 cut(s) 739, 1065, 1256, 1530
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 2 cut(s) 1349, 1539
FspBI CTAG 2 cut(s) 647, 1434
GlaI GCGC 1 cut(s) 456
GluI GCNGC 2 cut(s) 1349, 1539
GsaI CCCAGC 1 cut(s) 249
HaeII RGCGCY 1 cut(s) 458
HaeIII GGCC 3 cut(s) 118, 813, 1129
HapII CCGG 2 cut(s) 119, 333
HhaI GCGC 1 cut(s) 457
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HpaII CCGG 2 cut(s) 119, 333
HphI GGTGA 2 cut(s) 67, 440
Hpy166II GTNNAC 4 cut(s) 338, 625, 959, 1444
Hpy188I TCNGA 8 cut(s) 279, 358, 865, 981, 1059, 1134, 1147, 1519
Hpy188III TCNNGA 7 cut(s) 677, 849, 920, 1181, 1418, 1487, 1594
Hpy8I GTNNAC 4 cut(s) 338, 625, 959, 1444
Hpy99I CGWCG 2 cut(s) 55, 145
HpyAV CCTTC 4 cut(s) 73, 592, 1129, 1350
HpyCH4III ACNGT 6 cut(s) 55, 264, 402, 544, 1391, 1556
HpyCH4IV ACGT 2 cut(s) 885, 1075
HpyF10VI GCNNNNNNNGC 2 cut(s) 1434, 1538
HpyF3I CTNAG 1 cut(s) 1158
HpySE526I ACGT 2 cut(s) 885, 1075
HspAI GCGC 1 cut(s) 455
LmnI GCTCC 4 cut(s) 279, 358, 434, 992
Lsp1109I GCAGC 2 cut(s) 1335, 1550
LweI GCATC 3 cut(s) 524, 1207, 1447
MaeI CTAG 2 cut(s) 647, 1434
MaeII ACGT 2 cut(s) 885, 1075
MaeIII GTNAC 5 cut(s) 55, 520, 1261, 1525, 1607
MfeI CAATTG 1 cut(s) 962
MflI RGATCY 1 cut(s) 1024
MhlI GDGCHC 3 cut(s) 312, 627, 908
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 2 cut(s) 257, 1354
MnlI CCTC 9 cut(s) 104, 107, 186, 241, 676, 1095, 1140, 1290, 1557
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 1045, 1339
MseI TTAA 7 cut(s) 86, 363, 539, 764, 951, 1295, 1621
MslI CAYNNNNRTG 5 cut(s) 9, 200, 390, 913, 1218
MspA1I CMGCKG 1 cut(s) 1316
MspCI CTTAAG 1 cut(s) 362
MspI CCGG 2 cut(s) 119, 333
MspR9I CCNGG 2 cut(s) 512, 758
MunI CAATTG 1 cut(s) 962
Mva1269I GAATGC 1 cut(s) 1208
MvaI CCWGG 2 cut(s) 512, 758
MwoI GCNNNNNNNGC 2 cut(s) 1434, 1538
NlaIV GGNNCC 1 cut(s) 946
NmuCI GTSAC 3 cut(s) 55, 520, 1607
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 8, 620, 1536
NspV TTCGAA 1 cut(s) 555
PaeI GCATGC 1 cut(s) 8
PceI AGGCCT 1 cut(s) 813
PciI ACATGT 1 cut(s) 616
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 1 cut(s) 1208
PdmI GAANNNNTTC 2 cut(s) 1045, 1339
PflFI GACNNNGTC 1 cut(s) 402
PkrI GCNGC 2 cut(s) 1350, 1540
PleI GAGTC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 282
PscI ACATGT 1 cut(s) 616
PsiI TTATAA 1 cut(s) 1251
Psp6I CCWGG 2 cut(s) 510, 756
PspFI CCCAGC 1 cut(s) 245
PspGI CCWGG 2 cut(s) 510, 756
PspN4I GGNNCC 1 cut(s) 946
PspPI GGNCC 3 cut(s) 116, 398, 1127
PsrI GAACNNNNNNTAC 2 cut(s) 1033, 1065
PsuI RGATCY 1 cut(s) 1024
PsyI GACNNNGTC 1 cut(s) 402
PvuII CAGCTG 1 cut(s) 1316
RsaI GTAC 3 cut(s) 383, 717, 1558
RsaNI GTAC 3 cut(s) 382, 716, 1557
RseI CAYNNNNRTG 5 cut(s) 9, 200, 390, 913, 1218
Rsr2I CGGWCCG 1 cut(s) 398
RsrII CGGWCCG 1 cut(s) 398
SaqAI TTAA 7 cut(s) 86, 363, 539, 764, 951, 1295, 1621
SatI GCNGC 2 cut(s) 1349, 1539
Sau96I GGNCC 3 cut(s) 116, 398, 1127
ScaI AGTACT 1 cut(s) 717
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 2 cut(s) 512, 758
SduI GDGCHC 3 cut(s) 312, 627, 908
SfaNI GCATC 3 cut(s) 524, 1207, 1447
SfcI CTRYAG 1 cut(s) 930
SfuI TTCGAA 1 cut(s) 555
SinI GGWCC 1 cut(s) 398
SmiMI CAYNNNNRTG 5 cut(s) 9, 200, 390, 913, 1218
SmlI CTYRAG 2 cut(s) 224, 362
SmoI CTYRAG 2 cut(s) 224, 362
SphI GCATGC 1 cut(s) 8
SseBI AGGCCT 1 cut(s) 813
SsiI CCGC 5 cut(s) 374, 396, 592, 1196, 1283
SspI AATATT 1 cut(s) 802
SspMI CTAG 2 cut(s) 647, 1434
StuI AGGCCT 1 cut(s) 813
StyD4I CCNGG 2 cut(s) 510, 756
TaaI ACNGT 6 cut(s) 55, 264, 402, 544, 1391, 1556
TaiI ACGT 2 cut(s) 888, 1078
TaqI TCGA 7 cut(s) 288, 306, 426, 555, 636, 1182, 1361
TatI WGTACW 1 cut(s) 715
Tru1I TTAA 7 cut(s) 86, 363, 539, 764, 951, 1295, 1621
Tru9I TTAA 7 cut(s) 86, 363, 539, 764, 951, 1295, 1621
TscAI CASTG 4 cut(s) 196, 489, 549, 1396
TseFI GTSAC 3 cut(s) 55, 520, 1607
TseI GCWGC 2 cut(s) 1348, 1538
Tsp45I GTSAC 3 cut(s) 55, 520, 1607
TspDTI ATGAA 8 cut(s) 190, 594, 621, 726, 794, 1262, 1332, 1572
TspGWI ACGGA 2 cut(s) 44, 312
TspRI CASTG 4 cut(s) 196, 489, 549, 1396
Tth111I GACNNNGTC 1 cut(s) 402
Vha464I CTTAAG 1 cut(s) 362
VneI GTGCAC 1 cut(s) 623
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 4 cut(s) 167, 368, 1357, 1579
XceI RCATGY 3 cut(s) 8, 620, 1536
XcmI CCANNNNNNNNNTGG 1 cut(s) 1220
XmiI GTMKAC 1 cut(s) 958
XmnI GAANNNNTTC 2 cut(s) 1045, 1339
XspI CTAG 2 cut(s) 647, 1434
ZrmI AGTACT 1 cut(s) 717
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.