Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0002249

Physical Location & Seq

Genomic Coordinates Forward (+)

3364494 .. 3367662

Gene Structure 3169 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom11g04320.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1755 bp

ATGCAGGATGCAATGATGCAAGATATCAATATTCCCCCATATAGCAGTGCTTTAGAGTTTTCTCCTGATCCAATGGATTCATTCAGTAGTTCAAAGCAAACGCTATCAAAGGATGATGGTCACAGTGGTAGATTAGATAGTTATTATGGGTGCAGAAGAATCTTTGACGATGCTGAAAATTTCAGAAAAGATAAATGGGATGGTAAATACTTCTGCCTTGAGTTATACTGTCGGCCATGTTTCCCAGAGGAATACACTTTTAATGAATGGGAACATGATTTATCATGGAAGAACTGGCAAAGTCAAATGAACTGTGCTTCTGATGATAATTTGATGCACGAAAAATACCAGACGTCAGATTTTGCTTTTGAAGGATCCTTTTCACCGATAAGGTGCTTACTTAATTTCTACTTCTTCAGTTATCTTGTTTTGAGAAATTATATCTTCTTGATAGAGTCGTGGCATCTGTGCAGGTCTGCTACGGGCATCACAGACAAGTTCGACATCTTAGGTGAACCTTCATATTTCAAGCACCAAAAATCAGAAAAGTATCATGATTTTATGGTTCCCAATGGAGCAAGACGCCATGCAGTAGGAAGGAATTTTGATTTTGGTGGTGTAACCAGGCAACCAGATTGGTCTAGCTTTATGAATGAAAATGAAAGGGACAATTTGAGCTTGCTGAGGGACAATGCGATTGATAGTTCACTGTCAAAGCCAACCAGGAATTGGAGCAAGAGAAGACATGACAATGCATACGCTGACCCAGTATATCATTTGAATATAACTTCTACAGGAAAGACACAGATCAAAAGCAGGCATGTTGTTCAACCAGAGGATATTGCACATGGGTCAGGAACATGCACGAAGATGCCAGATTCGTTGAAGTTCAAACCATTCCACCACTCAAACTCTCCTCTCCAGGAAAAGTTTACCCCAAACTGCAATTGGTTCCCTGAGGAAGGATATCTGTCGGTTGACAATAATTCAGGTTGCAGTTCTTTCCATCAAAAGTCAGAGACAAACTGCCCATCTGCAGGCTCTAAAATTTTGTTTGGAGATCCTTTCAGTGCAAGTCCTGTCCCAGAATTACATCTCAATCCTAGATCTCATTTTAAACCCTGCGAACCTGTTGCAAGTTCACCTTCCGGCAATTTGATTTCTGGGAACTTCGAATTTCATGACTCTCCTATGACCCCTAAGATTGGTTTTGGACCAAGAAAACAAGAGTCCCATTACTCTCATGGCGAGACACCATCTCCAGATTTATCAGCGCAGGAAAGTGTCAGCAAAGATGAGGGAAGAAAAGCTGAATCGCAGCAATGCCAAAAATCTAAGCTGGAGGAAGAACATTGTATTCTGAACAATCTGTACACAGAAAATCAACGATGGAACAATAACAGTGAATTTGAGGAAGTAAAAGATGCAGCTTCGGGACTAACAGCAAGCATAAAACCAACCTTGCTTGAGCGGGTTGAGAAGACATCGTCATCTATGAACTCTCCAGCGAAATATGGAAGCATAATACAAAAGAAAGAGGATTACTGTGGTTACGAAATTCCTATCCCATGTCCAAATCACAACGGAGAAATGGAAGAGGCTGAGCCTCAGGAAACGAAGAAAGAAAGTAAACAGCAAAGTGATTTTGTTGACTCGTCCTGTCGGGTGATGATGCTTCAGAGCTATGTTCAGTTTCTTTGTGTGCAGAAGGTATTGGAGGAGGCAGCTGCACACAACGGCCTAAAGAAGATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

585

Amino Acids

66.81

Weight (kDa)

5.68

Isoelectric Point (pI)

53.1

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30480 AT2G30480 AT2G30480 AT2G30480 AT2G30480 AT2G30480 AT2G30480 AT2G30480 AT2G30480 AT2G30480 AT2G30480 AT2G30480 AT2G30480
fragaria_vesca	FvH4_3g40550 FvH4_3g40550
malus_domestica	MD11G1053100.v1.1 MD11G1053200.v1.1 MD11G1053300.v1.1
prunus_persica	Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1 Prupe.6G040800_v2.0.a1
pyrus_communis	pycom11g04320
rosa_chinensis	RchiOBHm_Chr5g0073231
rosa_laevigata	RLG00000036376
rosa_multiflora	Rmu_sc0001887.1_g000014
rosa_roxburghii	Rroxscaffold_1G00007960
rosa_rugosa	Rorug05G0422400 Rorug05G0422500
rosa_samantha	Rh5AG479200 Rh5BG499900 Rh5CG523100 Rh5DG512000
rosa_wichuraiana	Rw5G044550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	356
Acc36I	ACCTGC	1 cut(s)	462
AccB7I	CCANNNNNTGG	1 cut(s)	729
AccBSI	CCGCTC	1 cut(s)	1471
AciI	CCGC	1 cut(s)	1471
AclWI	GGATC	4 cut(s)	62, 369, 382, 1055
AcoI	YGGCCR	1 cut(s)	233
AcsI	RAATTY	6 cut(s)	178, 601, 1047, 1175, 1406, 1557
AcuI	CTGAAG	2 cut(s)	400, 1661
AcyI	GRCGYC	2 cut(s)	353, 583
AfaI	GTAC	1 cut(s)	1373
AfiI	CCNNNNNNNGG	4 cut(s)	729, 962, 1037, 1205
AgsI	TTSAA	7 cut(s)	93, 371, 529, 781, 830, 886, 892
AjnI	CCWGG	3 cut(s)	623, 722, 921
AluBI	AGCT	7 cut(s)	645, 678, 1310, 1339, 1430, 1683, 1727
AluI	AGCT	7 cut(s)	645, 678, 1310, 1339, 1430, 1683, 1727
Alw26I	GTCTC	2 cut(s)	1013, 1244
AlwI	GGATC	4 cut(s)	62, 369, 382, 1055
AoxI	GGCC	2 cut(s)	233, 1738
ApeKI	GCWGC	4 cut(s)	1318, 1427, 1724, 1727
ApoI	RAATTY	6 cut(s)	178, 601, 1047, 1175, 1406, 1557
AspLEI	GCGC	1 cut(s)	1276
AspS9I	GGNCC	1 cut(s)	1214
AsuHPI	GGTGA	4 cut(s)	375, 524, 1134, 1678
AsuII	TTCGAA	1 cut(s)	1173
AvaII	GGWCC	1 cut(s)	1214
AxyI	CCTNAGG	2 cut(s)	957, 1608
BamHI	GGATCC	1 cut(s)	374
BbsI	GAAGAC	2 cut(s)	748, 1487
BbvCI	CCTCAGC	1 cut(s)	683
BbvI	GCAGC	4 cut(s)	1330, 1439, 1714, 1736
BccI	CCATC	6 cut(s)	110, 194, 1014, 1039, 1264, 1383
BceAI	ACGGC	1 cut(s)	1753
BcgI	CGANNNNNNTGC	2 cut(s)	1115, 1149
BciT130I	CCWGG	3 cut(s)	625, 724, 923
BcoDI	GTCTC	2 cut(s)	1013, 1244
BfaI	CTAG	2 cut(s)	642, 1104
BfmI	CTRYAG	2 cut(s)	792, 1035
BfuAI	ACCTGC	1 cut(s)	462
BglII	AGATCT	1 cut(s)	1106
BisI	GCNGC	4 cut(s)	1319, 1428, 1725, 1728
BlpI	GCTNAGC	1 cut(s)	1602
BlsI	GCNGC	4 cut(s)	1320, 1429, 1726, 1729
Bme1390I	CCNGG	3 cut(s)	625, 724, 923
Bme18I	GGWCC	1 cut(s)	1214
BmgT120I	GGNCC	1 cut(s)	1214
BmiI	GGNNCC	3 cut(s)	376, 567, 953
BmrFI	CCNGG	3 cut(s)	625, 724, 923
BmrI	ACTGGG	1 cut(s)	761
BmsI	GCATC	8 cut(s)	6, 160, 324, 472, 495, 861, 1414, 1662
BmuI	ACTGGG	1 cut(s)	761
BpiI	GAAGAC	2 cut(s)	748, 1487
BpmI	CTGGAG	4 cut(s)	905, 1245, 1361, 1488
Bpu10I	CCTNAGC	1 cut(s)	683
Bpu1102I	GCTNAGC	1 cut(s)	1602
Bpu14I	TTCGAA	1 cut(s)	1173
BpuEI	CTTGAG	2 cut(s)	239, 1487
BsaHI	GRCGYC	2 cut(s)	353, 583
BsaXI	ACNNNNNCTCC	2 cut(s)	1243, 1273
Bsc4I	CCNNNNNNNGG	4 cut(s)	729, 962, 1037, 1205
Bse1I	ACTGG	2 cut(s)	299, 767
Bse21I	CCTNAGG	2 cut(s)	957, 1608
Bse3DI	GCAATG	2 cut(s)	18, 1328
BseBI	CCWGG	3 cut(s)	625, 724, 923
BseGI	GGATG	3 cut(s)	13, 118, 205
BseLI	CCNNNNNNNGG	4 cut(s)	729, 962, 1037, 1205
BseMI	GCAATG	2 cut(s)	18, 1328
BseMII	CTCAG	4 cut(s)	674, 948, 1593, 1622
BseNI	ACTGG	2 cut(s)	299, 767
BseRI	GAGGAG	2 cut(s)	906, 1733
BseXI	GCAGC	4 cut(s)	1330, 1439, 1714, 1736
BsgI	GTGCAG	4 cut(s)	172, 490, 1713, 1724
BshFI	GGCC	2 cut(s)	235, 1740
BsiSI	CCGG	1 cut(s)	1149
BslFI	GGGAC	5 cut(s)	680, 701, 1067, 1216, 1449
BslI	CCNNNNNNNGG	4 cut(s)	729, 962, 1037, 1205
BsmAI	GTCTC	2 cut(s)	1013, 1244
BsmFI	GGGAC	5 cut(s)	680, 701, 1067, 1216, 1449
BsnI	GGCC	2 cut(s)	235, 1740
Bsp119I	TTCGAA	1 cut(s)	1173
Bsp1407I	TGTACA	1 cut(s)	1371
Bsp143I	GATC	5 cut(s)	67, 374, 807, 1060, 1106
Bsp1720I	GCTNAGC	1 cut(s)	1602
BspACI	CCGC	1 cut(s)	1471
BspANI	GGCC	2 cut(s)	235, 1740
BspCNI	CTCAG	4 cut(s)	675, 949, 1594, 1621
BspHI	TCATGA	2 cut(s)	553, 1180
BspLI	GGNNCC	3 cut(s)	376, 567, 953
BspMAI	CTGCAG	1 cut(s)	1039
BspMI	ACCTGC	1 cut(s)	462
BspPI	GGATC	4 cut(s)	62, 369, 382, 1055
BspT104I	TTCGAA	1 cut(s)	1173
BsrBI	CCGCTC	1 cut(s)	1471
BsrDI	GCAATG	2 cut(s)	18, 1328
BsrGI	TGTACA	1 cut(s)	1371
BsrI	ACTGG	2 cut(s)	299, 767
BssMI	GATC	5 cut(s)	67, 374, 807, 1060, 1106
BssNI	GRCGYC	2 cut(s)	353, 583
Bst2UI	CCWGG	3 cut(s)	625, 724, 923
Bst4CI	ACNGT	6 cut(s)	125, 230, 314, 711, 1403, 1547
Bst6I	CTCTTC	1 cut(s)	1590
BstACI	GRCGYC	2 cut(s)	353, 583
BstAUI	TGTACA	1 cut(s)	1371
BstBI	TTCGAA	1 cut(s)	1173
BstC8I	GCNNGC	4 cut(s)	680, 818, 1039, 1447
BstDEI	CTNAG	7 cut(s)	508, 683, 957, 1200, 1335, 1602, 1608
BstENI	CCTNNNNNAGG	1 cut(s)	960
BstF5I	GGATG	3 cut(s)	13, 118, 205
BstHHI	GCGC	1 cut(s)	1276
BstKTI	GATC	5 cut(s)	70, 377, 810, 1063, 1109
BstMAI	GTCTC	2 cut(s)	1013, 1244
BstMBI	GATC	5 cut(s)	67, 374, 807, 1060, 1106
BstNI	CCWGG	3 cut(s)	625, 724, 923
BstNSI	RCATGY	2 cut(s)	824, 864
BstSCI	CCNGG	3 cut(s)	623, 722, 921
BstSFI	CTRYAG	2 cut(s)	792, 1035
BstV1I	GCAGC	4 cut(s)	1330, 1439, 1714, 1736
BstV2I	GAAGAC	2 cut(s)	748, 1487
BstX2I	RGATCY	3 cut(s)	374, 1060, 1106
BstYI	RGATCY	3 cut(s)	374, 1060, 1106
Bsu36I	CCTNAGG	2 cut(s)	957, 1608
BsuRI	GGCC	2 cut(s)	235, 1740
BtsCI	GGATG	3 cut(s)	13, 118, 205
BtsI	GCAGTG	1 cut(s)	52
BtsIMutI	CAGTG	5 cut(s)	52, 130, 707, 1075, 1408
BveI	ACCTGC	1 cut(s)	462
Cac8I	GCNNGC	4 cut(s)	680, 818, 1039, 1447
CciI	TCATGA	2 cut(s)	553, 1180
CfoI	GCGC	1 cut(s)	1276
Cfr13I	GGNCC	1 cut(s)	1214
CseI	GACGC	1 cut(s)	591
Csp6I	GTAC	1 cut(s)	1372
CviQI	GTAC	1 cut(s)	1372
DdeI	CTNAG	7 cut(s)	508, 683, 957, 1200, 1335, 1602, 1608
DpnI	GATC	5 cut(s)	69, 376, 809, 1062, 1108
DpnII	GATC	5 cut(s)	67, 374, 807, 1060, 1106
DraI	TTTAAA	1 cut(s)	1117
EaeI	YGGCCR	1 cut(s)	233
Eam1104I	CTCTTC	1 cut(s)	1590
EarI	CTCTTC	1 cut(s)	1590
Eco32I	GATATC	2 cut(s)	25, 968
Eco47I	GGWCC	1 cut(s)	1214
Eco57I	CTGAAG	2 cut(s)	400, 1661
Eco81I	CCTNAGG	2 cut(s)	957, 1608
EcoNI	CCTNNNNNAGG	1 cut(s)	960
EcoRII	CCWGG	3 cut(s)	623, 722, 921
EcoRV	GATATC	2 cut(s)	25, 968
EcoT22I	ATGCAT	1 cut(s)	757
FalI	AAGNNNNNCTT	2 cut(s)	1129, 1161
FaqI	GGGAC	5 cut(s)	680, 701, 1067, 1216, 1449
FauI	CCCGC	1 cut(s)	1464
Fnu4HI	GCNGC	4 cut(s)	1319, 1428, 1725, 1728
FokI	GGATG	3 cut(s)	20, 125, 212
Fsp4HI	GCNGC	4 cut(s)	1319, 1428, 1725, 1728
FspBI	CTAG	2 cut(s)	642, 1104
GlaI	GCGC	1 cut(s)	1275
GluI	GCNGC	4 cut(s)	1319, 1428, 1725, 1728
GsuI	CTGGAG	4 cut(s)	905, 1245, 1361, 1488
HaeIII	GGCC	2 cut(s)	235, 1740
HapII	CCGG	1 cut(s)	1149
HgaI	GACGC	1 cut(s)	591
HhaI	GCGC	1 cut(s)	1276
Hin1I	GRCGYC	2 cut(s)	353, 583
Hin6I	GCGC	1 cut(s)	1274
HinP1I	GCGC	1 cut(s)	1274
HincII	GTYRAC	2 cut(s)	979, 1651
HindII	GTYRAC	2 cut(s)	979, 1651
HinfI	GANTC	8 cut(s)	77, 159, 455, 878, 1184, 1229, 1313, 1652
HpaII	CCGG	1 cut(s)	1149
HphI	GGTGA	4 cut(s)	375, 524, 1134, 1678
Hpy166II	GTNNAC	8 cut(s)	515, 707, 933, 979, 1142, 1374, 1631, 1651
Hpy188I	TCNGA	7 cut(s)	185, 322, 358, 544, 1018, 1362, 1680
Hpy188III	TCNNGA	8 cut(s)	65, 448, 554, 855, 1181, 1262, 1434, 1610
Hpy8I	GTNNAC	8 cut(s)	515, 707, 933, 979, 1142, 1374, 1631, 1651
HpyAV	CCTTC	6 cut(s)	365, 528, 591, 956, 1155, 1702
HpyCH4III	ACNGT	6 cut(s)	125, 230, 314, 711, 1403, 1547
HpyCH4IV	ACGT	1 cut(s)	353
HpyF3I	CTNAG	7 cut(s)	508, 683, 957, 1200, 1335, 1602, 1608
HpySE526I	ACGT	1 cut(s)	353
Hsp92I	GRCGYC	2 cut(s)	353, 583
HspAI	GCGC	1 cut(s)	1274
Kzo9I	GATC	5 cut(s)	67, 374, 807, 1060, 1106
LmnI	GCTCC	2 cut(s)	575, 732
Lsp1109I	GCAGC	4 cut(s)	1330, 1439, 1714, 1736
LweI	GCATC	8 cut(s)	6, 160, 324, 472, 495, 861, 1414, 1662
MaeI	CTAG	2 cut(s)	642, 1104
MaeII	ACGT	1 cut(s)	353
MaeIII	GTNAC	3 cut(s)	119, 619, 1550
MalI	GATC	5 cut(s)	69, 376, 809, 1062, 1108
MbiI	CCGCTC	1 cut(s)	1471
MboI	GATC	5 cut(s)	67, 374, 807, 1060, 1106
MfeI	CAATTG	1 cut(s)	946
MflI	RGATCY	3 cut(s)	374, 1060, 1106
MlyI	GAGTC	4 cut(s)	464, 1178, 1238, 1646
Mph1103I	ATGCAT	1 cut(s)	757
MseI	TTAA	3 cut(s)	261, 402, 1116
MslI	CAYNNNNRTG	2 cut(s)	750, 869
MspA1I	CMGCKG	1 cut(s)	1727
MspI	CCGG	1 cut(s)	1149
MspR9I	CCNGG	3 cut(s)	625, 724, 923
MunI	CAATTG	1 cut(s)	946
MvaI	CCWGG	3 cut(s)	625, 724, 923
NdeII	GATC	5 cut(s)	67, 374, 807, 1060, 1106
NlaIV	GGNNCC	3 cut(s)	376, 567, 953
NmuCI	GTSAC	1 cut(s)	119
NsiI	ATGCAT	1 cut(s)	757
NspI	RCATGY	2 cut(s)	824, 864
NspV	TTCGAA	1 cut(s)	1173
PagI	TCATGA	2 cut(s)	553, 1180
PfeI	GAWTC	4 cut(s)	77, 159, 878, 1313
PflFI	GACNNNGTC	1 cut(s)	1486
PflMI	CCANNNNNTGG	1 cut(s)	729
PfoI	TCCNGGA	1 cut(s)	921
PkrI	GCNGC	4 cut(s)	1320, 1429, 1726, 1729
PleI	GAGTC	4 cut(s)	463, 1178, 1237, 1646
PpsI	GAGTC	4 cut(s)	463, 1178, 1237, 1646
Psp6I	CCWGG	3 cut(s)	623, 722, 921
PspGI	CCWGG	3 cut(s)	623, 722, 921
PspN4I	GGNNCC	3 cut(s)	376, 567, 953
PspPI	GGNCC	1 cut(s)	1214
PsrI	GAACNNNNNNTAC	2 cut(s)	1355, 1387
PstI	CTGCAG	1 cut(s)	1039
PsuI	RGATCY	3 cut(s)	374, 1060, 1106
PsyI	GACNNNGTC	1 cut(s)	1486
PvuII	CAGCTG	1 cut(s)	1727
RsaI	GTAC	1 cut(s)	1373
RsaNI	GTAC	1 cut(s)	1372
RseI	CAYNNNNRTG	2 cut(s)	750, 869
SaqAI	TTAA	3 cut(s)	261, 402, 1116
SatI	GCNGC	4 cut(s)	1319, 1428, 1725, 1728
Sau3AI	GATC	5 cut(s)	67, 374, 807, 1060, 1106
Sau96I	GGNCC	1 cut(s)	1214
SchI	GAGTC	4 cut(s)	464, 1178, 1238, 1646
ScrFI	CCNGG	3 cut(s)	625, 724, 923
SfaNI	GCATC	8 cut(s)	6, 160, 324, 472, 495, 861, 1414, 1662
SfcI	CTRYAG	2 cut(s)	792, 1035
SfuI	TTCGAA	1 cut(s)	1173
SinI	GGWCC	1 cut(s)	1214
SmiMI	CAYNNNNRTG	2 cut(s)	750, 869
SmlI	CTYRAG	2 cut(s)	218, 1466
SmoI	CTYRAG	2 cut(s)	218, 1466
SsiI	CCGC	1 cut(s)	1471
SspI	AATATT	1 cut(s)	31
SspMI	CTAG	2 cut(s)	642, 1104
StyD4I	CCNGG	3 cut(s)	623, 722, 921
TaaI	ACNGT	6 cut(s)	125, 230, 314, 711, 1403, 1547
TaiI	ACGT	1 cut(s)	356
TaqI	TCGA	2 cut(s)	501, 1173
TatI	WGTACW	1 cut(s)	1371
TfiI	GAWTC	4 cut(s)	77, 159, 878, 1313
Tru1I	TTAA	3 cut(s)	261, 402, 1116
Tru9I	TTAA	3 cut(s)	261, 402, 1116
TscAI	CASTG	5 cut(s)	52, 130, 714, 1075, 1408
TseFI	GTSAC	1 cut(s)	119
TseI	GCWGC	4 cut(s)	1318, 1427, 1724, 1727
Tsp45I	GTSAC	1 cut(s)	119
TspDTI	ATGAA	9 cut(s)	69, 279, 323, 510, 665, 669, 675, 1169, 1511
TspGWI	ACGGA	1 cut(s)	1599
TspRI	CASTG	5 cut(s)	52, 130, 714, 1075, 1408
Tth111I	GACNNNGTC	1 cut(s)	1486
Van91I	CCANNNNNTGG	1 cut(s)	729
VpaK11BI	GGWCC	1 cut(s)	1214
XagI	CCTNNNNNAGG	1 cut(s)	960
XapI	RAATTY	6 cut(s)	178, 601, 1047, 1175, 1406, 1557
XceI	RCATGY	2 cut(s)	824, 864
XcmI	CCANNNNNNNNNTGG	3 cut(s)	726, 945, 1241
XspI	CTAG	2 cut(s)	642, 1104
ZraI	GACGTC	1 cut(s)	354
Zsp2I	ATGCAT	1 cut(s)	757

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.