Rh5DG512000

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002249
Physical Location & Seq
Reverse (-)
79882289 .. 79890408
8120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG512000.1

Sequence Viewer

Length: 2145 bp
ATGAAAAAAACACTCGAGAAAGAAGATGTGGGTCGTAACCAGGCCCTCAAATCAGAGAAGATCAGAGAGGGCAGACATGCGGACCCGTCTATCGCTACTGCTCCTGATAATCACAGAAAGTCTTTCAATGAAATGTATTCTAAACCACATAACTCGAGGACACCTGTTGAGCAGCTTTCAGAGTCTTGTGTGCTTGATTTTTTCGGCAATGATGGATTGGAGGATGACCATGTAGAAGGAAGTCCAGTCCATGAAGCTCACGTTGCATTTTCAGTTGAAGGTTTGGGAAAAGTGGGAATGGAAACACCGGTCCATTCACCAAAACAACCTGGAAGGACCTTCTCCTATGATTGCTGCACACCATTGCACGGTGCAAGGAAAGTGAACTCATTCAAGAACCTGAAGCGTCTGGATGATATTGAAATTGAAATGGACACAGTAATGCAAGATATCACGATGCCCCTTAGTAGCAATGATTTAGAGTTCTCTGAGGATTTAATGGATTCAATCAGTAATCCAAAGAAGAAATTCTCCACTTTCAGAAATTGCACACAATATGATGGTCATGATAGTAAATTTGAAACCTATTATGGAGGCAGAAGAATATTTGACGATATTGAAAATGGCAACAAAGACAGATGGGATGGTAGGTATAACTACCTTGAGGAAGACTCTTTTAATGAATGGGAACATGATTTATCATTGGGGAGGCCAAGTGAAGTGAACCGTAGATCTGTTGATAATATGATGCATGGAGAGTATGGGATGTCAGATTTCACTTTTGAAGAACCTTTTTCCCCAACGAGGTTTTGTTCAAGCATAACAGACAAGTTCAACATCTTAGAGTCACCTTCATACTCGAAGCACCAAGTATTAGAGAATGATCTTGATTTTATATTTTCCACCGGGGCAAGAGAGCATACACGAAGGAAATTTAATTTTGGAGGTGTAGCCAGCCGACCTGATTGGTCTTGCTTTGCAACTGAATATGATAGGGATAATGCGAGCCTACTGAGTGAAGAGTCATGCTCATCAAGTGCAGTGAGGGTCAATGCAACTGATGATTCGCTGCCAAAGTCAACCAGAAAACACAGTAAGAGAAGACATGGCAATTCATGTGCCGACCCTGAACATAAATATCATGTGGACATAACTTCTATGGAAAGGACACAAGACAGAAGCAGGGATTTTTTTCAACAGAAGAATATTGCACATGGATCAGGGAGATGTACAAATATGTCAAAATCATCAAAGTTTAAACCCTCCCACCACTCAAACACTCCTTTCCGTGAAAAGTCAACCCCAAACAGCAATTGGAGGTCTGAGGAAAGATACATGTCAGTTGATATAGATTCGGGTTCCAGTTCTTTCCATCAAAATTCCTGTCCATCAGCAGGCTCTAAGCCTTTGTCTCGAGATCCTTTCGGTGCATTCGATTGCCTAGAATTACATGTGGATGCCAGACCTTCAGAACACTGTGAACCTGTTGCGAGTTCACCTTCAGGCAGTTTTATCTCTCAGAAATTTGCATTTCATGACTCTCCTCCTGTAACCTCTAACATTGGGTGTGGACCAACAAAACCAAATCTTTATCCATGTTCACATGATCAGACCCCATCTCCTGATTTGTCAGCACAAGAAAGTTTTAGCAAGGCCGAGGAGGGAAAAGTGAAATTCCAGCCCAAAGAAAAATCTAGACAGGAGGAAGAAAATGCTATTCTGAACAACTTGCTCACAGAAAGTGTAGATGCAGCAAGTGCTTTACGATCAAAGAACAAGTACAGTGTATGCAAGAAAGCAAAGGATGCAGATTCAGGACGAAATGAGGGAAGTATAAAAGCAACCTATATGCCTAACCATACTGAGGAGGCATCATCGTTTTTGAAGAGTCCAGATAAAATTCAAAACACAGTGGATGAAAAAGAAGAATCCCATCACTCTGAAATTCCTCTTCCATGTCGAAGTCGGAACAAAGAGATGGAAGATTCTGAGCCTCAGGAAAGAAAGACTTTCCTTAAACAGCAAAGTGAATTCGTTGACTCATCTTCTCAGGTTATGATGCTTCAGAGCTATGTTCTTCAGTTTCTTTGTGTGCAGAAGGTATTGAAGGACGCAGCTGCACAGAATAGCATGAAGAAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

714

Amino Acids

80.83

Weight (kDa)

5.86

Isoelectric Point (pI)

62.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 80
AclWI GGATC 2 cut(s) 1225, 1412
AcsI RAATTY 9 cut(s) 527, 575, 932, 1378, 1523, 1673, 1899, 1944, 2030
AcuI CTGAAG 5 cut(s) 422, 1452, 1485, 2048, 2063
AfaI GTAC 2 cut(s) 1231, 1781
AfiI CCNNNNNNNGG 4 cut(s) 368, 804, 1394, 1864
AflIII ACRYGT 2 cut(s) 1335, 1450
AgeI ACCGGT 1 cut(s) 307
AjnI CCWGG 2 cut(s) 39, 328
AluBI AGCT 4 cut(s) 175, 257, 2070, 2117
AluI AGCT 4 cut(s) 175, 257, 2070, 2117
Alw26I GTCTC 1 cut(s) 1416
AlwI GGATC 2 cut(s) 1225, 1412
Ama87I CYCGRG 3 cut(s) 14, 154, 1413
AoxI GGCC 3 cut(s) 42, 710, 1653
ApeKI GCWGC 6 cut(s) 172, 354, 1069, 1751, 2114, 2117
ApoI RAATTY 9 cut(s) 527, 575, 932, 1378, 1523, 1673, 1899, 1944, 2030
ArsI GACNNNNNNTTYG 2 cut(s) 1369, 1401
AsiGI ACCGGT 1 cut(s) 307
Asp700I GAANNNNTTC 2 cut(s) 389, 527
AspS9I GGNCC 5 cut(s) 43, 82, 310, 336, 1571
AsuC2I CCSGG 1 cut(s) 907
AsuHPI GGTGA 3 cut(s) 309, 840, 1488
AvaI CYCGRG 3 cut(s) 14, 154, 1413
AvaII GGWCC 4 cut(s) 82, 310, 336, 1571
AxyI CCTNAGG 1 cut(s) 1995
BbsI GAAGAC 2 cut(s) 675, 1108
BbvI GCAGC 6 cut(s) 184, 341, 1056, 1763, 2104, 2126
BccI CCATC 9 cut(s) 206, 554, 633, 638, 1380, 1396, 1624, 1941, 1972
BciT130I CCWGG 2 cut(s) 41, 330
BclI TGATCA 1 cut(s) 1606
BcnI CCSGG 1 cut(s) 907
BcoDI GTCTC 1 cut(s) 1416
BfaI CTAG 2 cut(s) 1442, 1695
BglII AGATCT 1 cut(s) 731
BisI GCNGC 6 cut(s) 173, 355, 1070, 1752, 2115, 2118
BlsI GCNGC 6 cut(s) 174, 356, 1071, 1753, 2116, 2119
Bme1390I CCNGG 3 cut(s) 41, 330, 907
Bme18I GGWCC 4 cut(s) 82, 310, 336, 1571
BmeT110I CYCGRG 3 cut(s) 14, 154, 1413
BmgT120I GGNCC 5 cut(s) 43, 82, 310, 336, 1571
BmiI GGNNCC 2 cut(s) 84, 1360
BmrFI CCNGG 3 cut(s) 41, 330, 907
BmsI GCATC 7 cut(s) 447, 738, 1447, 1738, 1795, 1880, 2049
BpiI GAAGAC 2 cut(s) 675, 1108
BplI GAGNNNNNCTC 4 cut(s) 656, 688, 1013, 1045
BpuEI CTTGAG 1 cut(s) 683
BpuMI CCSGG 1 cut(s) 907
BsaJI CCNNGG 2 cut(s) 906, 1656
BsaWI WCCGGW 1 cut(s) 307
BsaXI ACNNNNNCTCC 4 cut(s) 700, 730, 1603, 1633
Bsc4I CCNNNNNNNGG 4 cut(s) 368, 804, 1394, 1864
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 2 cut(s) 245, 1362
Bse21I CCTNAGG 1 cut(s) 1995
Bse3DI GCAATG 3 cut(s) 214, 362, 478
BseBI CCWGG 2 cut(s) 41, 330
BseDI CCNNGG 2 cut(s) 906, 1656
BseGI GGATG 7 cut(s) 229, 418, 649, 771, 1462, 1810, 1921
BseLI CCNNNNNNNGG 4 cut(s) 368, 804, 1394, 1864
BseMI GCAATG 3 cut(s) 214, 362, 478
BseMII CTCAG 8 cut(s) 480, 1004, 1314, 1532, 1854, 1980, 2009, 2063
BseNI ACTGG 2 cut(s) 245, 1362
BseRI GAGGAG 3 cut(s) 1533, 1673, 1880
BseXI GCAGC 6 cut(s) 184, 341, 1056, 1763, 2104, 2126
BsgI GTGCAG 4 cut(s) 340, 1059, 2103, 2114
BshFI GGCC 3 cut(s) 44, 712, 1655
BshTI ACCGGT 1 cut(s) 307
BsiHKCI CYCGRG 3 cut(s) 14, 154, 1413
BsiSI CCGG 2 cut(s) 308, 906
BslI CCNNNNNNNGG 4 cut(s) 368, 804, 1394, 1864
BsmAI GTCTC 1 cut(s) 1416
BsmI GAATGC 1 cut(s) 1430
BsnI GGCC 3 cut(s) 44, 712, 1655
BsoBI CYCGRG 3 cut(s) 14, 154, 1413
Bsp1407I TGTACA 1 cut(s) 1229
Bsp143I GATC 7 cut(s) 60, 731, 883, 1217, 1417, 1606, 1766
BspACI CCGC 1 cut(s) 80
BspANI GGCC 3 cut(s) 44, 712, 1655
BspCNI CTCAG 8 cut(s) 481, 1005, 1315, 1531, 1855, 1981, 2008, 2062
BspHI TCATGA 2 cut(s) 565, 1534
BspLI GGNNCC 2 cut(s) 84, 1360
BspPI GGATC 2 cut(s) 1225, 1412
BsrDI GCAATG 3 cut(s) 214, 362, 478
BsrFI RCCGGY 1 cut(s) 307
BsrGI TGTACA 1 cut(s) 1229
BsrI ACTGG 2 cut(s) 245, 1362
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 2 cut(s) 906, 1656
BssMI GATC 7 cut(s) 60, 731, 883, 1217, 1417, 1606, 1766
Bst2UI CCWGG 2 cut(s) 41, 330
Bst4CI ACNGT 7 cut(s) 371, 439, 728, 1094, 1478, 1784, 1912
Bst6I CTCTTC 3 cut(s) 1014, 1880, 1956
BstAPI GCANNNNNTGC 2 cut(s) 1757, 1805
BstAUI TGTACA 1 cut(s) 1229
BstC8I GCNNGC 3 cut(s) 955, 1006, 1396
BstF5I GGATG 7 cut(s) 229, 418, 649, 771, 1462, 1810, 1921
BstKTI GATC 7 cut(s) 63, 734, 886, 1220, 1420, 1609, 1769
BstMAI GTCTC 1 cut(s) 1416
BstMBI GATC 7 cut(s) 60, 731, 883, 1217, 1417, 1606, 1766
BstMWI GCNNNNNNNGC 3 cut(s) 263, 1757, 1805
BstNI CCWGG 2 cut(s) 41, 330
BstNSI RCATGY 3 cut(s) 80, 1339, 1454
BstSCI CCNGG 3 cut(s) 39, 328, 905
BstV1I GCAGC 6 cut(s) 184, 341, 1056, 1763, 2104, 2126
BstV2I GAAGAC 2 cut(s) 675, 1108
BstX2I RGATCY 2 cut(s) 731, 1417
BstYI RGATCY 2 cut(s) 731, 1417
Bsu36I CCTNAGG 1 cut(s) 1995
BsuRI GGCC 3 cut(s) 44, 712, 1655
BtsCI GGATG 7 cut(s) 229, 418, 649, 771, 1462, 1810, 1921
BtsI GCAGTG 1 cut(s) 1047
BtsIMutI CAGTG 4 cut(s) 1047, 1474, 1789, 1917
Cac8I GCNNGC 3 cut(s) 955, 1006, 1396
CciI TCATGA 2 cut(s) 565, 1534
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 5 cut(s) 43, 82, 310, 336, 1571
CseI GACGC 2 cut(s) 395, 2120
Csp6I GTAC 2 cut(s) 1230, 1780
CspAI ACCGGT 1 cut(s) 307
CviQI GTAC 2 cut(s) 1230, 1780
DpnI GATC 7 cut(s) 62, 733, 885, 1219, 1419, 1608, 1768
DpnII GATC 7 cut(s) 60, 731, 883, 1217, 1417, 1606, 1766
DraI TTTAAA 1 cut(s) 1258
Eam1104I CTCTTC 3 cut(s) 1014, 1880, 1956
EarI CTCTTC 3 cut(s) 1014, 1880, 1956
Eco32I GATATC 1 cut(s) 451
Eco47I GGWCC 4 cut(s) 82, 310, 336, 1571
Eco57I CTGAAG 5 cut(s) 422, 1452, 1485, 2048, 2063
Eco81I CCTNAGG 1 cut(s) 1995
Eco88I CYCGRG 3 cut(s) 14, 154, 1413
EcoO109I RGGNCCY 2 cut(s) 43, 336
EcoRI GAATTC 1 cut(s) 2030
EcoRII CCWGG 2 cut(s) 39, 328
EcoRV GATATC 1 cut(s) 451
EcoT22I ATGCAT 1 cut(s) 753
FalI AAGNNNNNCTT 2 cut(s) 1993, 2025
FbaI TGATCA 1 cut(s) 1606
Fnu4HI GCNGC 6 cut(s) 173, 355, 1070, 1752, 2115, 2118
FokI GGATG 7 cut(s) 236, 425, 656, 778, 1469, 1817, 1928
Fsp4HI GCNGC 6 cut(s) 173, 355, 1070, 1752, 2115, 2118
FspBI CTAG 2 cut(s) 1442, 1695
GluI GCNGC 6 cut(s) 173, 355, 1070, 1752, 2115, 2118
HaeIII GGCC 3 cut(s) 44, 712, 1655
HapII CCGG 2 cut(s) 308, 906
HgaI GACGC 2 cut(s) 395, 2120
HincII GTYRAC 3 cut(s) 1080, 1299, 2038
HindII GTYRAC 3 cut(s) 1080, 1299, 2038
HpaII CCGG 2 cut(s) 308, 906
HphI GGTGA 3 cut(s) 309, 840, 1488
HpyCH4III ACNGT 7 cut(s) 371, 439, 728, 1094, 1478, 1784, 1912
HpyCH4IV ACGT 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 1757, 1805
HpySE526I ACGT 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 1606
Kzo9I GATC 7 cut(s) 60, 731, 883, 1217, 1417, 1606, 1766
LmnI GCTCC 1 cut(s) 106
Lsp1109I GCAGC 6 cut(s) 184, 341, 1056, 1763, 2104, 2126
LweI GCATC 7 cut(s) 447, 738, 1447, 1738, 1795, 1880, 2049
MaeI CTAG 2 cut(s) 1442, 1695
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 35, 846, 1549
MalI GATC 7 cut(s) 62, 733, 885, 1219, 1419, 1608, 1768
MboI GATC 7 cut(s) 60, 731, 883, 1217, 1417, 1606, 1766
MfeI CAATTG 1 cut(s) 1312
MflI RGATCY 2 cut(s) 731, 1417
MlyI GAGTC 7 cut(s) 191, 665, 854, 1031, 1532, 1897, 2033
MmeI TCCRAC 1 cut(s) 1946
Mph1103I ATGCAT 1 cut(s) 753
MroXI GAANNNNTTC 2 cut(s) 389, 527
MseI TTAA 5 cut(s) 497, 678, 936, 1257, 2016
MslI CAYNNNNRTG 2 cut(s) 440, 1455
MspA1I CMGCKG 1 cut(s) 2117
MspI CCGG 2 cut(s) 308, 906
MspR9I CCNGG 3 cut(s) 41, 330, 907
MssI GTTTAAAC 1 cut(s) 1258
MunI CAATTG 1 cut(s) 1312
Mva1269I GAATGC 1 cut(s) 1430
MvaI CCWGG 2 cut(s) 41, 330
MwoI GCNNNNNNNGC 3 cut(s) 263, 1757, 1805
NciI CCSGG 1 cut(s) 907
NdeII GATC 7 cut(s) 60, 731, 883, 1217, 1417, 1606, 1766
NlaIV GGNNCC 2 cut(s) 84, 1360
NmeAIII GCCGAG 1 cut(s) 1681
NmuCI GTSAC 1 cut(s) 846
NsiI ATGCAT 1 cut(s) 753
NspI RCATGY 3 cut(s) 80, 1339, 1454
PaeR7I CTCGAG 3 cut(s) 14, 154, 1413
PagI TCATGA 2 cut(s) 565, 1534
PciI ACATGT 2 cut(s) 1335, 1450
PcsI WCGNNNNNNNCGW 1 cut(s) 1431
PctI GAATGC 1 cut(s) 1430
PdmI GAANNNNTTC 2 cut(s) 389, 527
PfeI GAWTC 6 cut(s) 503, 1064, 1352, 1811, 1928, 1985
PinAI ACCGGT 1 cut(s) 307
PkrI GCNGC 6 cut(s) 174, 356, 1071, 1753, 2116, 2119
PleI GAGTC 7 cut(s) 190, 665, 853, 1030, 1532, 1896, 2033
PmeI GTTTAAAC 1 cut(s) 1258
PpsI GAGTC 7 cut(s) 190, 665, 853, 1030, 1532, 1896, 2033
PpuMI RGGWCCY 1 cut(s) 336
PscI ACATGT 2 cut(s) 1335, 1450
Psp5II RGGWCCY 1 cut(s) 336
Psp6I CCWGG 2 cut(s) 39, 328
PspGI CCWGG 2 cut(s) 39, 328
PspN4I GGNNCC 2 cut(s) 84, 1360
PspPI GGNCC 5 cut(s) 43, 82, 310, 336, 1571
PspPPI RGGWCCY 1 cut(s) 336
PspXI VCTCGAGB 1 cut(s) 154
PsuI RGATCY 2 cut(s) 731, 1417
PvuII CAGCTG 1 cut(s) 2117
RsaI GTAC 2 cut(s) 1231, 1781
RsaNI GTAC 2 cut(s) 1230, 1780
RseI CAYNNNNRTG 2 cut(s) 440, 1455
SaqAI TTAA 5 cut(s) 497, 678, 936, 1257, 2016
SatI GCNGC 6 cut(s) 173, 355, 1070, 1752, 2115, 2118
Sau3AI GATC 7 cut(s) 60, 731, 883, 1217, 1417, 1606, 1766
Sau96I GGNCC 5 cut(s) 43, 82, 310, 336, 1571
SchI GAGTC 7 cut(s) 191, 665, 854, 1031, 1532, 1897, 2033
ScrFI CCNGG 3 cut(s) 41, 330, 907
SfaNI GCATC 7 cut(s) 447, 738, 1447, 1738, 1795, 1880, 2049
Sfr274I CTCGAG 3 cut(s) 14, 154, 1413
SinI GGWCC 4 cut(s) 82, 310, 336, 1571
SlaI CTCGAG 3 cut(s) 14, 154, 1413
SmiMI CAYNNNNRTG 2 cut(s) 440, 1455
SmlI CTYRAG 4 cut(s) 14, 154, 662, 1413
SmoI CTYRAG 4 cut(s) 14, 154, 662, 1413
SsiI CCGC 1 cut(s) 80
SspI AATATT 2 cut(s) 606, 1207
SspMI CTAG 2 cut(s) 1442, 1695
StyD4I CCNGG 3 cut(s) 39, 328, 905
TaaI ACNGT 7 cut(s) 371, 439, 728, 1094, 1478, 1784, 1912
TaiI ACGT 1 cut(s) 264
TaqI TCGA 6 cut(s) 15, 155, 860, 1414, 1434, 1960
TatI WGTACW 2 cut(s) 1229, 1779
TfiI GAWTC 6 cut(s) 503, 1064, 1352, 1811, 1928, 1985
Tru1I TTAA 5 cut(s) 497, 678, 936, 1257, 2016
Tru9I TTAA 5 cut(s) 497, 678, 936, 1257, 2016
TscAI CASTG 4 cut(s) 1047, 1481, 1789, 1917
TseFI GTSAC 1 cut(s) 846
TseI GCWGC 6 cut(s) 172, 354, 1069, 1751, 2114, 2117
Tsp45I GTSAC 1 cut(s) 846
TspDTI ATGAA 8 cut(s) 17, 144, 267, 696, 843, 1104, 1523, 1932
TspGWI ACGGA 1 cut(s) 1277
TspRI CASTG 4 cut(s) 1047, 1481, 1789, 1917
VpaK11BI GGWCC 4 cut(s) 82, 310, 336, 1571
XapI RAATTY 9 cut(s) 527, 575, 932, 1378, 1523, 1673, 1899, 1944, 2030
XbaI TCTAGA 1 cut(s) 1694
XceI RCATGY 3 cut(s) 80, 1339, 1454
XcmI CCANNNNNNNNNTGG 1 cut(s) 1311
XhoI CTCGAG 3 cut(s) 14, 154, 1413
XmnI GAANNNNTTC 2 cut(s) 389, 527
XspI CTAG 2 cut(s) 1442, 1695
Zsp2I ATGCAT 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.