pycom11g05300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0013406
Physical Location & Seq
Reverse (-)
4098521 .. 4100778
2258 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g05300.1

Sequence Viewer

Length: 759 bp
ATGTCAGAGCTCTTTCGATCGCTCGTCTTGAACAGAAGGTTCGATAACGTGACGCTACGAGTATTGGAATCAGCATTGGTTTCGAAGGACGTAAAGTCGTCGATGGAAGTCAGGTCCGGCTTCAGGCAGTTCATGAAATCTGAATCCCTCTCCATTCTGCGAGAAACTGCAGAGAAAAATGTGGAGGAGAAGCTTCTGGTTCTTGAGTTTCTCGTTCGCGCTTTCGCACTAGTAGGCGATGTTGAGAGTTGTTTGGCTTTGAGATATGAGGCATTGCTTTTGCGGGACGTCAAATCTTCGACGAATCCGTGGCTACAAGTTTCTTATACGGAATGGCTGAATTTTGCTCACCAGTCAAGAGATAGTGGTTTCTATTCAGTTGCAGGAAAGGCATGTGAACATGCATTGGATTGCATTAAGAGGAAATCTACTGAAGACCGTAAAACAGATGAAGTTAACGTTATGAAGAAATCTACTGAAGACCCAAAAACAGATGAAGTCCTCGAAAGTGTGCAAGCAATTGAAAAGATAAAGAGACTCAAGGATTGTGCCATGGCATCTGCTTCTTCCCACTCTGTTCAAGCGCAGACGGCAGACTACTTGAAAAAGAGACTAAAGGAGAAGAGCACGGAGCGTCTTTTAGATTTCAAGGAGCCAAAATGTGTAGCAAGCACTTTGTTCCGAAATGGAATCAGAAGGCGGAATCTACGAAAATTGAACATGAGTCGAAGCTTGCTGGGGATGACTAATGAATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

253

Amino Acids

28.87

Weight (kDa)

9.31

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07060 AT1G07060 AT1G07060 AT1G07060
fragaria_vesca FvH4_3g39530
malus_domestica MD11G1063300.v1.1
prunus_persica Prupe.6G050500_v2.0.a1
pyrus_communis pycom11g05300
rosa_chinensis RchiOBHm_Chr5g0071301
rosa_laevigata RLG00000036200
rosa_multiflora Rmu_sc0006990.1_g000010
rosa_roxburghii Rroxscaffold_1G00009840
rosa_rugosa Rorug05G0411200
rosa_samantha Rh5AG467500 Rh5BG486000 Rh5CG510400 Rh5DG497200
rosa_wichuraiana Rw5G043430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 291
AccII CGCG 1 cut(s) 219
AciI CCGC 2 cut(s) 283, 700
AclI AACGTT 1 cut(s) 459
AcsI RAATTY 1 cut(s) 340
AcuI CTGAAG 3 cut(s) 106, 453, 498
AcyI GRCGYC 1 cut(s) 288
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 6 cut(s) 31, 524, 581, 604, 649, 718
AhdI GACNNNNNGTC 1 cut(s) 94
AhlI ACTAGT 1 cut(s) 229
AluBI AGCT 3 cut(s) 10, 193, 732
AluI AGCT 3 cut(s) 10, 193, 732
Alw21I GWGCWC 2 cut(s) 12, 629
Alw26I GTCTC 2 cut(s) 529, 604
ApoI RAATTY 1 cut(s) 340
AspLEI GCGC 2 cut(s) 221, 586
AspS9I GGNCC 1 cut(s) 114
AsuHPI GGTGA 1 cut(s) 341
AsuII TTCGAA 1 cut(s) 83
AvaII GGWCC 1 cut(s) 114
BanII GRGCYC 1 cut(s) 12
BbsI GAAGAC 2 cut(s) 441, 486
Bbv12I GWGCWC 2 cut(s) 12, 629
BccI CCATC 1 cut(s) 97
BceAI ACGGC 1 cut(s) 606
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BcoDI GTCTC 2 cut(s) 529, 604
BcuI ACTAGT 1 cut(s) 229
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 168
Bme18I GGWCC 1 cut(s) 114
BmeRI GACNNNNNGTC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 1 cut(s) 654
BmsI GCATC 1 cut(s) 566
BpiI GAAGAC 2 cut(s) 441, 486
Bpu14I TTCGAA 1 cut(s) 83
BpuEI CTTGAG 2 cut(s) 224, 524
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 308, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 272
BseDI CCNNGG 2 cut(s) 308, 552
BseGI GGATG 1 cut(s) 747
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMI GCAATG 1 cut(s) 272
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 200
BseYI CCCAGC 1 cut(s) 736
Bsh1236I CGCG 1 cut(s) 219
Bsh1285I CGRYCG 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 20
BsiHKAI GWGCWC 2 cut(s) 12, 629
BsiSI CCGG 1 cut(s) 117
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 1 cut(s) 123
BsmAI GTCTC 2 cut(s) 529, 604
BsmFI GGGAC 1 cut(s) 299
Bsp119I TTCGAA 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 12, 629
Bsp143I GATC 1 cut(s) 17
Bsp19I CCATGG 1 cut(s) 552
BspACI CCGC 2 cut(s) 283, 700
BspFNI CGCG 1 cut(s) 219
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 654
BspMAI CTGCAG 1 cut(s) 172
BspQI GCTCTTC 1 cut(s) 617
BspT104I TTCGAA 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 272
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 308, 552
BssMI GATC 1 cut(s) 17
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 1 cut(s) 552
Bst4CI ACNGT 1 cut(s) 440
Bst6I CTCTTC 1 cut(s) 617
BstACI GRCGYC 1 cut(s) 288
BstBI TTCGAA 1 cut(s) 83
BstC8I GCNNGC 3 cut(s) 516, 670, 734
BstDSI CCRYGG 2 cut(s) 308, 552
BstF5I GGATG 1 cut(s) 747
BstFNI CGCG 1 cut(s) 219
BstHHI GCGC 2 cut(s) 221, 586
BstKTI GATC 1 cut(s) 20
BstMAI GTCTC 2 cut(s) 529, 604
BstMBI GATC 1 cut(s) 17
BstMCI CGRYCG 1 cut(s) 20
BstMWI GCNNNNNNNGC 2 cut(s) 389, 590
BstNSI RCATGY 2 cut(s) 396, 404
BstSFI CTRYAG 1 cut(s) 168
BstUI CGCG 1 cut(s) 219
BstV2I GAAGAC 2 cut(s) 441, 486
BtgI CCRYGG 2 cut(s) 308, 552
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 747
Cac8I GCNNGC 3 cut(s) 516, 670, 734
CciI TCATGA 1 cut(s) 132
CfoI GCGC 2 cut(s) 221, 586
Cfr13I GGNCC 1 cut(s) 114
CseI GACGC 2 cut(s) 61, 623
CviAII CATG 5 cut(s) 133, 393, 401, 553, 721
CviJI RGCY 8 cut(s) 10, 120, 193, 257, 313, 337, 655, 732
CviKI_1 RGCY 8 cut(s) 10, 120, 193, 257, 313, 337, 655, 732
DpnI GATC 1 cut(s) 19
DpnII GATC 1 cut(s) 17
DriI GACNNNNNGTC 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 617
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 617
EciI GGCGGA 1 cut(s) 715
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 114
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 3 cut(s) 106, 453, 498
EcoICRI GAGCTC 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 1 cut(s) 406
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 5 cut(s) 136, 396, 404, 556, 724
FaiI YATR 9 cut(s) 134, 267, 327, 394, 402, 464, 554, 722, 757
FaqI GGGAC 1 cut(s) 299
FatI CATG 5 cut(s) 132, 392, 400, 552, 720
FauI CCCGC 1 cut(s) 276
FokI GGATG 1 cut(s) 754
FriOI GRGCYC 1 cut(s) 12
FspBI CTAG 1 cut(s) 230
GlaI GCGC 2 cut(s) 220, 585
GsaI CCCAGC 1 cut(s) 740
HapII CCGG 1 cut(s) 117
HgaI GACGC 2 cut(s) 61, 623
HhaI GCGC 2 cut(s) 221, 586
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 5 cut(s) 136, 396, 404, 556, 724
Hin6I GCGC 2 cut(s) 219, 584
HinP1I GCGC 2 cut(s) 219, 584
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 2 cut(s) 191, 730
HinfI GANTC 8 cut(s) 68, 143, 304, 537, 690, 703, 724, 752
HpaI GTTAAC 1 cut(s) 457
HpaII CCGG 1 cut(s) 117
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 2 cut(s) 398, 457
Hpy188I TCNGA 4 cut(s) 7, 142, 683, 695
Hpy188III TCNNGA 4 cut(s) 28, 133, 203, 357
Hpy8I GTNNAC 2 cut(s) 398, 457
Hpy99I CGWCG 2 cut(s) 103, 304
HpyAV CCTTC 3 cut(s) 30, 79, 690
HpyCH4III ACNGT 1 cut(s) 440
HpyCH4IV ACGT 4 cut(s) 48, 90, 288, 459
HpyCH4V TGCA 5 cut(s) 170, 383, 404, 414, 514
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 590
HpySE526I ACGT 4 cut(s) 48, 90, 288, 459
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 5 cut(s) 136, 396, 404, 556, 724
HspAI GCGC 2 cut(s) 219, 584
KspAI GTTAAC 1 cut(s) 457
Kzo9I GATC 1 cut(s) 17
LguI GCTCTTC 1 cut(s) 617
LmnI GCTCC 2 cut(s) 631, 652
LpnPI CCDG 7 cut(s) 97, 109, 130, 182, 365, 369, 722
LweI GCATC 1 cut(s) 566
MaeI CTAG 1 cut(s) 230
MaeII ACGT 4 cut(s) 48, 90, 288, 459
MaeIII GTNAC 1 cut(s) 49
MalI GATC 1 cut(s) 19
MboI GATC 1 cut(s) 17
MboII GAAGA 6 cut(s) 288, 446, 478, 491, 558, 634
MfeI CAATTG 1 cut(s) 519
MhlI GDGCHC 2 cut(s) 12, 629
MluCI AATT 3 cut(s) 340, 519, 713
MlyI GAGTC 2 cut(s) 531, 733
MnlI CCTC 5 cut(s) 158, 178, 262, 414, 512
Mph1103I ATGCAT 1 cut(s) 406
MseI TTAA 2 cut(s) 417, 456
MspI CCGG 1 cut(s) 117
MunI CAATTG 1 cut(s) 519
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 2 cut(s) 389, 590
NcoI CCATGG 1 cut(s) 552
NdeII GATC 1 cut(s) 17
NlaIII CATG 5 cut(s) 136, 396, 404, 556, 724
NlaIV GGNNCC 1 cut(s) 654
NmuCI GTSAC 1 cut(s) 49
NsiI ATGCAT 1 cut(s) 406
NspI RCATGY 2 cut(s) 396, 404
NspV TTCGAA 1 cut(s) 83
PagI TCATGA 1 cut(s) 132
PciSI GCTCTTC 1 cut(s) 617
PcsI WCGNNNNNNNCGW 1 cut(s) 305
PfeI GAWTC 6 cut(s) 68, 143, 304, 690, 703, 752
Ple19I CGATCG 1 cut(s) 20
PleI GAGTC 2 cut(s) 531, 732
PpsI GAGTC 2 cut(s) 531, 732
Psp124BI GAGCTC 1 cut(s) 12
Psp1406I AACGTT 1 cut(s) 459
PspFI CCCAGC 1 cut(s) 736
PspN4I GGNNCC 1 cut(s) 654
PspPI GGNCC 1 cut(s) 114
PstI CTGCAG 1 cut(s) 172
PvuI CGATCG 1 cut(s) 20
SacI GAGCTC 1 cut(s) 12
SapI GCTCTTC 1 cut(s) 617
SaqAI TTAA 2 cut(s) 417, 456
Sau3AI GATC 1 cut(s) 17
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 2 cut(s) 531, 733
SduI GDGCHC 2 cut(s) 12, 629
SetI ASST 9 cut(s) 12, 41, 51, 93, 116, 195, 291, 462, 734
SfaNI GCATC 1 cut(s) 566
SfcI CTRYAG 1 cut(s) 168
SfuI TTCGAA 1 cut(s) 83
SinI GGWCC 1 cut(s) 114
SmlI CTYRAG 2 cut(s) 203, 539
SmoI CTYRAG 2 cut(s) 203, 539
SpeI ACTAGT 1 cut(s) 229
Sse9I AATT 3 cut(s) 340, 519, 713
SsiI CCGC 2 cut(s) 283, 700
SspMI CTAG 1 cut(s) 230
SstI GAGCTC 1 cut(s) 12
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 1 cut(s) 440
TaiI ACGT 4 cut(s) 51, 93, 291, 462
TaqI TCGA 7 cut(s) 16, 42, 83, 101, 299, 504, 727
TasI AATT 3 cut(s) 340, 519, 713
TfiI GAWTC 6 cut(s) 68, 143, 304, 690, 703, 752
Tru1I TTAA 2 cut(s) 417, 456
Tru9I TTAA 2 cut(s) 417, 456
TseFI GTSAC 1 cut(s) 49
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 5 cut(s) 121, 149, 465, 479, 510
TspGWI ACGGA 3 cut(s) 297, 344, 644
VpaK11BI GGWCC 1 cut(s) 114
XapI RAATTY 1 cut(s) 340
XceI RCATGY 2 cut(s) 396, 404
XspI CTAG 1 cut(s) 230
ZraI GACGTC 1 cut(s) 289
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.