pycom11g06090

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010550
Physical Location & Seq
Reverse (-)
4708417 .. 4711885
3469 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1803 bp
ATGTTATTTTCGGGAGATGCTAAACATTTGTTAGCCGTTAATGTATGCGAACGTCTCGTAACCAAAACTCAATTCATTTCTTCCTTTCTCTGTTCTAATGAAATAAACTGGAGAAAGTATTCAAAAATGTCTAATAAGCAGTCTTTCAATGTCTGCTTTTGCTTCCAGAGAAAATTTAGGCTCTGGATGGCAGAGCCTCCTGAAGATATTAAGAACGTTTTTTATCTATACGCGCAAAATGGCACCATGACCATTGATGACCTGCAGAAATTTCTGATTGAGTTTCAAGGAGAATCCAGTGCTACCAAGGAAGATGCTCAGGCCATTTTCGACAGTCTCAAGCATCTCAACATCTTTCAACGAAAGGAACTCGACCTTGGTGCCTTCTTTCGATATCTCCTTGGTGACCTTAATCCTCCTCTCTCTTCAAAGGTGCACCATGACATGAATTCTCCATTGGCTCATTATTTCCTATTTACCGGCCACAACTCTTACTTAAAAGGAAATCAACTCAGCAGTAACAGTAGTGTTCGTCCGATAATTAACGCGCTGCGACAAGGTGTTAGAGTTATTGAATTAGACTTGTGGCAGGGTTCTAAGAAAAATGACGTGGTGGTTCGTCATGGAGGGACACTTACAAGTCCGGTGGATCTCATCAAATGTTTGCGCGCGATCAAGGAAAACGCTTTTGTTGCTTCTGAGTACCCTGTTGTCATAACATTTGAAGACCACCTACATTCAAATCTTCAAGCCAAAGTGGCAACGATGCTCACTGAGACATTTGGTGACATGCTGTACTCTCCCGATTCAGAATTCTTAAGGGAATTCCCTTCACCGGCATCATTGAAGAGGAGAATTTTGATTTCTACCAAGCCACCGAAGTATCCTGAATCGCAGAAACCTGGAGATCAATCTGGATCCACTGATCATAAGCAGGAAAGCGAACAAGATGATGAAGAAGATGAAGATAAAGACGAGGAGAATGCTATTCCGGAATATAAACAGTTGATTGCGATTCACGCAGGGAAGCCGAAACGTGGGTTAGATAAATGGCATATCGATCCAAATAAAGTCAGACGTCTTAGCTTGAACGAGCAACAACTCAAAAATGAAACAAGAACTCGTGGATCCGACATTGTCAGGTTTACTCAGAGGAATTTGTTGAGGGTTTCTCCAAAGGGTACACGTTTGAACTCATCAAATTATAATCCTATGCTTGCGTGGACTCATGGAGCTCAAATGGTGGCATTCAATATGCAAGGACATGGAAAGTACCTTTGGATTATGGAAGGAATGTTCAGAGCAAATGGAGGTTGTGGCTATGTGAAGAAACCTGAATTTTTGCTGGCTAGCCCAAATAATGAGGTTTTTGACCCTAATGCATCTTTACCGGTGAAAAAAACTTTGAAGGTAAAAGTATACATGGGTGACGGATGGCGTTTAGATTTCCATCGCACACACTTTGATCTATGTTCTCCACCGGACTTCTACGTGAGGGTTGGAATTGCGGGAGTCCCGAGCGACAAAACAAAAATGATGGAAACCGAACCAATACAGGACCAATGGGTGCCAGTTTGGAACAAGGAGTTCACCTTCCCATTGAGGGTTCCAGAGCTGGCTGTGCTTAGAGTTGAAGTCAATGAGTATGACACTCCGGCCAGACATGACTTTGGTGGCCAAACATGTCTGCCAATACGAGAGCTCAGAACTGGGATCCGAGCTGTTCCTCTGCACAGCGAAAGAGGTCAAAAGTATACATCTGTGAGGCTTCTGATGAGATTTGAATTGACAAATGAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004620 GO:0004629 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0008081 GO:0008150 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009553 GO:0009555 GO:0009556 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010600 GO:0010601 GO:0010817 GO:0016020 GO:0016298 GO:0016787 GO:0016788 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032350 GO:0032352 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0034293 GO:0042221 GO:0042578 GO:0042742 GO:0043207 GO:0043934 GO:0044464 GO:0044703 GO:0046885 GO:0046886 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071944 GO:0090354 GO:0090355 GO:0090567 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1903046
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

601

Amino Acids

68.88

Weight (kDa)

8.54

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 149 - 291 6.6e-51 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y PF00387 360 - 447 4e-28 Phosphatidylinositol-specific phospholipase C, Y domain
C2 PF00168 468 - 570 1.6e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1206
AatII GACGTC 1 cut(s) 1081
Acc36I ACCTGC 1 cut(s) 270
AccB1I GGYRCC 3 cut(s) 242, 380, 1567
AccI GTMKAC 2 cut(s) 1419, 1754
AccII CGCG 4 cut(s) 233, 548, 669, 671
AccIII TCCGGA 1 cut(s) 991
AciI CCGC 1 cut(s) 1508
AclI AACGTT 1 cut(s) 216
AclWI GGATC 8 cut(s) 657, 912, 925, 1055, 1122, 1135, 1708, 1721
AcoI YGGCCR 3 cut(s) 481, 1656, 1675
AcsI RAATTY 8 cut(s) 173, 269, 448, 812, 824, 855, 1156, 1337
AcuI CTGAAG 1 cut(s) 222
AcyI GRCGYC 1 cut(s) 1078
AfaI GTAC 4 cut(s) 704, 797, 1183, 1274
AfiI CCNNNNNNNGG 3 cut(s) 835, 1037, 1603
AflII CTTAAG 1 cut(s) 817
AflIII ACRYGT 2 cut(s) 1184, 1682
AgeI ACCGGT 1 cut(s) 1390
AjiI CACGTC 1 cut(s) 610
AjnI CCWGG 1 cut(s) 901
AjuI GAANNNNNNNTTGG 8 cut(s) 56, 88, 360, 392, 440, 472, 1261, 1293
AloI GAACNNNNNNTCC 2 cut(s) 1280, 1312
AluBI AGCT 5 cut(s) 1086, 1235, 1615, 1702, 1721
AluI AGCT 5 cut(s) 1086, 1235, 1615, 1702, 1721
Alw21I GWGCWC 3 cut(s) 438, 1237, 1704
Alw26I GTCTC 3 cut(s) 59, 341, 770
Alw44I GTGCAC 1 cut(s) 434
AlwI GGATC 8 cut(s) 657, 912, 925, 1055, 1122, 1135, 1708, 1721
Ama87I CYCGRG 1 cut(s) 1516
Aor13HI TCCGGA 1 cut(s) 991
AoxI GGCC 4 cut(s) 321, 481, 1656, 1675
ApaLI GTGCAC 1 cut(s) 434
ApeKI GCWGC 1 cut(s) 550
ApoI RAATTY 8 cut(s) 173, 269, 448, 812, 824, 855, 1156, 1337
AsiGI ACCGGT 1 cut(s) 1390
Asp700I GAANNNNTTC 1 cut(s) 118
AspLEI GCGC 4 cut(s) 235, 550, 669, 671
AspS9I GGNCC 1 cut(s) 1558
AsuHPI GGTGA 6 cut(s) 416, 797, 825, 1405, 1439, 1582
AsuNHI GCTAGC 1 cut(s) 1349
AvaI CYCGRG 1 cut(s) 1516
AvaII GGWCC 1 cut(s) 1558
BaeGI GKGCMC 1 cut(s) 438
BaeI ACNNNNGTAYC 2 cut(s) 866, 899
BalI TGGCCA 1 cut(s) 1677
BamHI GGATCC 3 cut(s) 917, 1127, 1713
BanI GGYRCC 3 cut(s) 242, 380, 1567
BanII GRGCYC 2 cut(s) 1237, 1704
BarI GAAGNNNNNNTAC 2 cut(s) 717, 749
BauI CACGAG 1 cut(s) 1122
BbsI GAAGAC 1 cut(s) 732
Bbv12I GWGCWC 3 cut(s) 438, 1237, 1704
BbvI GCAGC 1 cut(s) 537
BccI CCATC 4 cut(s) 181, 1428, 1458, 1531
BceAI ACGGC 1 cut(s) 20
BcgI CGANNNNNNTGC 4 cut(s) 362, 396, 965, 999
BciT130I CCWGG 1 cut(s) 903
BciVI GTATCC 1 cut(s) 894
BclI TGATCA 1 cut(s) 925
BcoDI GTCTC 3 cut(s) 59, 341, 770
BfaI CTAG 1 cut(s) 1350
BfmI CTRYAG 1 cut(s) 263
BfrI CTTAAG 1 cut(s) 817
BfuAI ACCTGC 1 cut(s) 270
BfuI GTATCC 1 cut(s) 894
BglI GCCNNNNNGGC 1 cut(s) 758
BisI GCNGC 1 cut(s) 551
BlsI GCNGC 1 cut(s) 552
Bme1390I CCNGG 1 cut(s) 903
Bme18I GGWCC 1 cut(s) 1558
BmeT110I CYCGRG 1 cut(s) 1516
BmgBI CACGTC 1 cut(s) 610
BmgT120I GGNCC 1 cut(s) 1558
BmiI GGNNCC 7 cut(s) 244, 382, 919, 1129, 1569, 1608, 1715
BmrFI CCNGG 1 cut(s) 903
BmrI ACTGGG 1 cut(s) 1719
BmsI GCATC 6 cut(s) 7, 304, 352, 756, 848, 1391
BmtI GCTAGC 1 cut(s) 1353
BmuI ACTGGG 1 cut(s) 1719
BpiI GAAGAC 1 cut(s) 732
BplI GAGNNNNNCTC 2 cut(s) 1156, 1188
BpmI CTGGAG 2 cut(s) 130, 924
Bpu10I CCTNAGC 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 323
Bsa29I ATCGAT 1 cut(s) 1059
BsaAI YACGTR 1 cut(s) 1492
BsaBI GATNNNNATC 1 cut(s) 1449
BsaHI GRCGYC 1 cut(s) 1078
BsaJI CCNNGG 3 cut(s) 306, 376, 400
BsaWI WCCGGW 4 cut(s) 643, 991, 1390, 1480
Bsc4I CCNNNNNNNGG 3 cut(s) 835, 1037, 1603
Bse118I RCCGGY 3 cut(s) 479, 835, 1390
Bse1I ACTGG 4 cut(s) 113, 297, 1571, 1714
Bse8I GATNNNNATC 1 cut(s) 1449
BseAI TCCGGA 1 cut(s) 991
BseBI CCWGG 1 cut(s) 903
BseCI ATCGAT 1 cut(s) 1059
BseDI CCNNGG 3 cut(s) 306, 376, 400
BseGI GGATG 2 cut(s) 192, 1439
BseJI GATNNNNATC 1 cut(s) 1449
BseLI CCNNNNNNNGG 3 cut(s) 835, 1037, 1603
BseMII CTCAG 6 cut(s) 332, 526, 690, 765, 1163, 1717
BseNI ACTGG 4 cut(s) 113, 297, 1571, 1714
BsePI GCGCGC 1 cut(s) 667
BseRI GAGGAG 3 cut(s) 408, 865, 992
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 1 cut(s) 537
BsgI GTGCAG 1 cut(s) 1715
Bsh1236I CGCG 4 cut(s) 233, 548, 669, 671
BshFI GGCC 4 cut(s) 323, 483, 1658, 1677
BshNI GGYRCC 3 cut(s) 242, 380, 1567
BshTI ACCGGT 1 cut(s) 1390
BshVI ATCGAT 1 cut(s) 1059
BsiHKAI GWGCWC 3 cut(s) 438, 1237, 1704
BsiHKCI CYCGRG 1 cut(s) 1516
BsiSI CCGG 7 cut(s) 480, 644, 836, 992, 1391, 1481, 1655
BslFI GGGAC 2 cut(s) 643, 1499
BslI CCNNNNNNNGG 3 cut(s) 835, 1037, 1603
BsmAI GTCTC 3 cut(s) 59, 341, 770
BsmBI CGTCTC 1 cut(s) 59
BsmFI GGGAC 2 cut(s) 643, 1499
BsmI GAATGC 2 cut(s) 988, 1247
BsnI GGCC 4 cut(s) 323, 483, 1658, 1677
BsoBI CYCGRG 1 cut(s) 1516
Bsp1286I GDGCHC 3 cut(s) 438, 1237, 1704
Bsp13I TCCGGA 1 cut(s) 991
Bsp143I GATC 9 cut(s) 649, 672, 907, 917, 925, 1060, 1127, 1465, 1713
BspACI CCGC 1 cut(s) 1508
BspANI GGCC 4 cut(s) 323, 483, 1658, 1677
BspCNI CTCAG 6 cut(s) 331, 525, 691, 766, 1162, 1716
BspDI ATCGAT 1 cut(s) 1059
BspEI TCCGGA 1 cut(s) 991
BspFNI CGCG 4 cut(s) 233, 548, 669, 671
BspLI GGNNCC 7 cut(s) 244, 382, 919, 1129, 1569, 1608, 1715
BspMAI CTGCAG 1 cut(s) 267
BspMI ACCTGC 1 cut(s) 270
BspOI GCTAGC 1 cut(s) 1353
BspPI GGATC 8 cut(s) 657, 912, 925, 1055, 1122, 1135, 1708, 1721
BspT107I GGYRCC 3 cut(s) 242, 380, 1567
BspTI CTTAAG 1 cut(s) 817
BsrFI RCCGGY 3 cut(s) 479, 835, 1390
BsrI ACTGG 4 cut(s) 113, 297, 1571, 1714
BssAI RCCGGY 3 cut(s) 479, 835, 1390
BssECI CCNNGG 3 cut(s) 306, 376, 400
BssHII GCGCGC 1 cut(s) 667
BssMI GATC 9 cut(s) 649, 672, 907, 917, 925, 1060, 1127, 1465, 1713
BssNAI GTATAC 2 cut(s) 1420, 1755
BssNI GRCGYC 1 cut(s) 1078
BssSI CACGAG 1 cut(s) 1122
BssT1I CCWWGG 3 cut(s) 306, 376, 400
Bst1107I GTATAC 2 cut(s) 1420, 1755
Bst2BI CACGAG 1 cut(s) 1122
Bst2UI CCWGG 1 cut(s) 903
Bst4CI ACNGT 3 cut(s) 335, 524, 1005
Bst6I CTCTTC 2 cut(s) 430, 842
BstACI GRCGYC 1 cut(s) 1078
BstAFI CTTAAG 1 cut(s) 817
BstBAI YACGTR 1 cut(s) 1492
BstC8I GCNNGC 5 cut(s) 669, 1218, 1347, 1351, 1617
BstDEI CTNAG 9 cut(s) 318, 512, 597, 699, 774, 1082, 1149, 1625, 1703
BstEII GGTNACC 1 cut(s) 404
BstF5I GGATG 2 cut(s) 192, 1439
BstFNI CGCG 4 cut(s) 233, 548, 669, 671
BstHHI GCGC 4 cut(s) 235, 550, 669, 671
BstKTI GATC 9 cut(s) 652, 675, 910, 920, 928, 1063, 1130, 1468, 1716
BstMAI GTCTC 3 cut(s) 59, 341, 770
BstMBI GATC 9 cut(s) 649, 672, 907, 917, 925, 1060, 1127, 1465, 1713
BstMWI GCNNNNNNNGC 4 cut(s) 692, 758, 1019, 1621
BstNI CCWGG 1 cut(s) 903
BstNSI RCATGY 2 cut(s) 793, 1686
BstPI GGTNACC 1 cut(s) 404
BstSCI CCNGG 1 cut(s) 901
BstSFI CTRYAG 1 cut(s) 263
BstSLI GKGCMC 1 cut(s) 438
BstUI CGCG 4 cut(s) 233, 548, 669, 671
BstV1I GCAGC 1 cut(s) 537
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 4 cut(s) 649, 917, 1127, 1713
BstYI RGATCY 4 cut(s) 649, 917, 1127, 1713
BstZ17I GTATAC 2 cut(s) 1420, 1755
Bsu15I ATCGAT 1 cut(s) 1059
BsuI GTATCC 1 cut(s) 894
BsuRI GGCC 4 cut(s) 323, 483, 1658, 1677
BsuTUI ATCGAT 1 cut(s) 1059
BtgZI GCGATG 1 cut(s) 1436
BtrI CACGTC 1 cut(s) 610
BtsCI GGATG 2 cut(s) 192, 1439
BtsIMutI CAGTG 3 cut(s) 304, 771, 921
BveI ACCTGC 1 cut(s) 270
Cac8I GCNNGC 5 cut(s) 669, 1218, 1347, 1351, 1617
CfoI GCGC 4 cut(s) 235, 550, 669, 671
Cfr10I RCCGGY 3 cut(s) 479, 835, 1390
Cfr13I GGNCC 1 cut(s) 1558
ClaI ATCGAT 1 cut(s) 1059
Csp6I GTAC 4 cut(s) 703, 796, 1182, 1273
CspAI ACCGGT 1 cut(s) 1390
CspCI CAANNNNNGTGG 2 cut(s) 627, 662
CviQI GTAC 4 cut(s) 703, 796, 1182, 1273
DdeI CTNAG 9 cut(s) 318, 512, 597, 699, 774, 1082, 1149, 1625, 1703
DpnI GATC 9 cut(s) 651, 674, 909, 919, 927, 1062, 1129, 1467, 1715
DpnII GATC 9 cut(s) 649, 672, 907, 917, 925, 1060, 1127, 1465, 1713
EaeI YGGCCR 3 cut(s) 481, 1656, 1675
Eam1104I CTCTTC 2 cut(s) 430, 842
EarI CTCTTC 2 cut(s) 430, 842
Ecl136II GAGCTC 2 cut(s) 1235, 1702
Eco130I CCWWGG 3 cut(s) 306, 376, 400
Eco24I GRGCYC 2 cut(s) 1237, 1704
Eco32I GATATC 1 cut(s) 395
Eco47I GGWCC 1 cut(s) 1558
Eco53kI GAGCTC 2 cut(s) 1235, 1702
Eco57I CTGAAG 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 1516
Eco91I GGTNACC 1 cut(s) 404
EcoICRI GAGCTC 2 cut(s) 1235, 1702
EcoO65I GGTNACC 1 cut(s) 404
EcoRI GAATTC 3 cut(s) 448, 812, 824
EcoRII CCWGG 1 cut(s) 901
EcoRV GATATC 1 cut(s) 395
EcoT14I CCWWGG 3 cut(s) 306, 376, 400
EcoT22I ATGCAT 1 cut(s) 1384
EcoT38I GRGCYC 2 cut(s) 1237, 1704
ErhI CCWWGG 3 cut(s) 306, 376, 400
Esp3I CGTCTC 1 cut(s) 59
FaqI GGGAC 2 cut(s) 643, 1499
FauI CCCGC 1 cut(s) 1501
FbaI TGATCA 1 cut(s) 925
FblI GTMKAC 2 cut(s) 1419, 1754
Fnu4HI GCNGC 1 cut(s) 551
FokI GGATG 2 cut(s) 199, 1446
FriOI GRGCYC 2 cut(s) 1237, 1704
Fsp4HI GCNGC 1 cut(s) 551
FspBI CTAG 1 cut(s) 1350
GlaI GCGC 4 cut(s) 234, 549, 668, 670
GluI GCNGC 1 cut(s) 551
GsuI CTGGAG 2 cut(s) 130, 924
HaeIII GGCC 4 cut(s) 323, 483, 1658, 1677
HapII CCGG 7 cut(s) 480, 644, 836, 992, 1391, 1481, 1655
HhaI GCGC 4 cut(s) 235, 550, 669, 671
Hin1I GRCGYC 1 cut(s) 1078
Hin6I GCGC 4 cut(s) 233, 548, 667, 669
HinP1I GCGC 4 cut(s) 233, 548, 667, 669
HinfI GANTC 6 cut(s) 293, 806, 890, 1015, 1225, 1512
HpaII CCGG 7 cut(s) 480, 644, 836, 992, 1391, 1481, 1655
HphI GGTGA 6 cut(s) 416, 797, 825, 1405, 1439, 1582
Hpy166II GTNNAC 7 cut(s) 436, 1146, 1184, 1224, 1420, 1590, 1755
Hpy8I GTNNAC 7 cut(s) 436, 1146, 1184, 1224, 1420, 1590, 1755
HpyAV CCTTC 5 cut(s) 394, 840, 1283, 1402, 1603
HpyCH4III ACNGT 3 cut(s) 335, 524, 1005
HpyCH4IV ACGT 7 cut(s) 52, 216, 609, 1036, 1078, 1186, 1491
HpyCH4V TGCA 5 cut(s) 265, 436, 1258, 1382, 1732
HpyF10VI GCNNNNNNNGC 4 cut(s) 692, 758, 1019, 1621
HpyF3I CTNAG 9 cut(s) 318, 512, 597, 699, 774, 1082, 1149, 1625, 1703
HpySE526I ACGT 7 cut(s) 52, 216, 609, 1036, 1078, 1186, 1491
Hsp92I GRCGYC 1 cut(s) 1078
HspAI GCGC 4 cut(s) 233, 548, 667, 669
Kpn2I TCCGGA 1 cut(s) 991
Ksp22I TGATCA 1 cut(s) 925
Kzo9I GATC 9 cut(s) 649, 672, 907, 917, 925, 1060, 1127, 1465, 1713
LmnI GCTCC 1 cut(s) 1232
Lsp1109I GCAGC 1 cut(s) 537
LweI GCATC 6 cut(s) 7, 304, 352, 756, 848, 1391
MaeI CTAG 1 cut(s) 1350
MaeII ACGT 7 cut(s) 52, 216, 609, 1036, 1078, 1186, 1491
MaeIII GTNAC 5 cut(s) 58, 404, 518, 785, 1427
MalI GATC 9 cut(s) 651, 674, 909, 919, 927, 1062, 1129, 1467, 1715
MboI GATC 9 cut(s) 649, 672, 907, 917, 925, 1060, 1127, 1465, 1713
MflI RGATCY 4 cut(s) 649, 917, 1127, 1713
MhlI GDGCHC 3 cut(s) 438, 1237, 1704
MlsI TGGCCA 1 cut(s) 1677
MluNI TGGCCA 1 cut(s) 1677
MlyI GAGTC 2 cut(s) 1219, 1521
MmeI TCCRAC 2 cut(s) 1155, 1480
Mox20I TGGCCA 1 cut(s) 1677
Mph1103I ATGCAT 1 cut(s) 1384
MroI TCCGGA 1 cut(s) 991
MroXI GAANNNNTTC 1 cut(s) 118
MscI TGGCCA 1 cut(s) 1677
MseI TTAA 7 cut(s) 39, 210, 411, 497, 543, 818, 1801
Msp20I TGGCCA 1 cut(s) 1677
MspCI CTTAAG 1 cut(s) 817
MspI CCGG 7 cut(s) 480, 644, 836, 992, 1391, 1481, 1655
MspR9I CCNGG 1 cut(s) 903
Mva1269I GAATGC 2 cut(s) 988, 1247
MvaI CCWGG 1 cut(s) 903
MvnI CGCG 4 cut(s) 233, 548, 669, 671
MwoI GCNNNNNNNGC 4 cut(s) 692, 758, 1019, 1621
NdeII GATC 9 cut(s) 649, 672, 907, 917, 925, 1060, 1127, 1465, 1713
NheI GCTAGC 1 cut(s) 1349
NlaIV GGNNCC 7 cut(s) 244, 382, 919, 1129, 1569, 1608, 1715
NmuCI GTSAC 3 cut(s) 404, 785, 1427
NsiI ATGCAT 1 cut(s) 1384
NspI RCATGY 2 cut(s) 793, 1686
PauI GCGCGC 1 cut(s) 667
PciI ACATGT 1 cut(s) 1682
PctI GAATGC 2 cut(s) 988, 1247
PdmI GAANNNNTTC 1 cut(s) 118
PfeI GAWTC 4 cut(s) 293, 806, 890, 1015
PflFI GACNNNGTC 1 cut(s) 1136
PinAI ACCGGT 1 cut(s) 1390
PkrI GCNGC 1 cut(s) 552
PleI GAGTC 2 cut(s) 1219, 1520
PpsI GAGTC 2 cut(s) 1219, 1520
Ppu21I YACGTR 1 cut(s) 1492
PscI ACATGT 1 cut(s) 1682
PsiI TTATAA 1 cut(s) 1206
Psp124BI GAGCTC 2 cut(s) 1237, 1704
Psp1406I AACGTT 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 901
PspEI GGTNACC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 901
PspN4I GGNNCC 7 cut(s) 244, 382, 919, 1129, 1569, 1608, 1715
PspPI GGNCC 1 cut(s) 1558
PstI CTGCAG 1 cut(s) 267
PsuI RGATCY 4 cut(s) 649, 917, 1127, 1713
PsyI GACNNNGTC 1 cut(s) 1136
PteI GCGCGC 1 cut(s) 667
RsaI GTAC 4 cut(s) 704, 797, 1183, 1274
RsaNI GTAC 4 cut(s) 703, 796, 1182, 1273
SacI GAGCTC 2 cut(s) 1237, 1704
SaqAI TTAA 7 cut(s) 39, 210, 411, 497, 543, 818, 1801
SatI GCNGC 1 cut(s) 551
Sau3AI GATC 9 cut(s) 649, 672, 907, 917, 925, 1060, 1127, 1465, 1713
Sau96I GGNCC 1 cut(s) 1558
SchI GAGTC 2 cut(s) 1219, 1521
ScrFI CCNGG 1 cut(s) 903
SduI GDGCHC 3 cut(s) 438, 1237, 1704
SfaNI GCATC 6 cut(s) 7, 304, 352, 756, 848, 1391
SfcI CTRYAG 1 cut(s) 263
SinI GGWCC 1 cut(s) 1558
SmlI CTYRAG 2 cut(s) 338, 817
SmoI CTYRAG 2 cut(s) 338, 817
SsiI CCGC 1 cut(s) 1508
SspMI CTAG 1 cut(s) 1350
SstI GAGCTC 2 cut(s) 1237, 1704
StyD4I CCNGG 1 cut(s) 901
StyI CCWWGG 3 cut(s) 306, 376, 400
TaaI ACNGT 3 cut(s) 335, 524, 1005
TaiI ACGT 7 cut(s) 55, 219, 612, 1039, 1081, 1189, 1494
TaqI TCGA 4 cut(s) 330, 372, 391, 1059
TatI WGTACW 1 cut(s) 795
TfiI GAWTC 4 cut(s) 293, 806, 890, 1015
Tru1I TTAA 7 cut(s) 39, 210, 411, 497, 543, 818, 1801
Tru9I TTAA 7 cut(s) 39, 210, 411, 497, 543, 818, 1801
TscAI CASTG 3 cut(s) 304, 778, 928
TseFI GTSAC 3 cut(s) 404, 785, 1427
TseI GCWGC 1 cut(s) 550
Tsp45I GTSAC 3 cut(s) 404, 785, 1427
TspDTI ATGAA 6 cut(s) 64, 114, 461, 969, 978, 1125
TspGWI ACGGA 1 cut(s) 1446
TspRI CASTG 3 cut(s) 304, 778, 928
Tth111I GACNNNGTC 1 cut(s) 1136
Vha464I CTTAAG 1 cut(s) 817
VneI GTGCAC 1 cut(s) 434
VpaK11BI GGWCC 1 cut(s) 1558
XapI RAATTY 8 cut(s) 173, 269, 448, 812, 824, 855, 1156, 1337
XceI RCATGY 2 cut(s) 793, 1686
XmiI GTMKAC 2 cut(s) 1419, 1754
XmnI GAANNNNTTC 1 cut(s) 118
XspI CTAG 1 cut(s) 1350
ZraI GACGTC 1 cut(s) 1079
Zsp2I ATGCAT 1 cut(s) 1384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.