pycom11g08230

nucleotidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007641
Physical Location & Seq
Forward (+)
6344557 .. 6346998
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 963 bp
ATGATGATAGCGACACTTTTCCAGTTAAACGCGCTTCCGACTCCTTCTTTTCTTTCACCTTACCCTACTAAAATATCCTTTCTCTCTATCTCTCTCTCTCTCTGCAACGTCGGTCATTCGGATCTGCCTTCTAGAAAACAGATGGAAAATCAGGTTCAATATCACCAATATGAGTGCCTTCTATTTGATCTAGACGACACCCTTTACCCTCTGAGTTCTGGAATTTCAGTACAAATCACTAAGAATATTAAAGAATATATGATTCAAAAGCTTGGCATGGACGAAAGTGAAGTTCCCGAATTGTGCATTTCTTTGTACAAGGATTATGGGACAACAATGGCCGGTCTTAAGGCTCAGGGCTATAACTTCGATTACGATGACTTTCATAGTTTTGTTCATGGGAGGTTGCCCTACGAGTTGCTTACTCCTGATCCTGTCCTGAGGGGTCTTTTACTTAGTCTGCCTATCCGCAAAGTAATTTTCACGAATTCGGATAAGAAACATGCGGATACTGTGCTCAAAAGGCTTGGAATAGAGGACTGCTTTGAGTCAGTTATATGCTTTGAGACAGTGAATCCCACCAACAATGCCGATGATTCTGTTGGTGCAGAAGAAACTGAACACACTGAACAACCAAATACTGGTGCGGTGGTTCCAAAGACCCCGGTTGTCTGCAAACCATTCGAGCTTGCATACGAAGAAGCCTTTAAATTTGCAAACATCGAACCTCAAAGAACATTATTCTTCGATGATAGCATTCGCAATATACAAACTGCAAAGCAGTGTGGCCTCCATACAGTATTGGTTGGCACCTCCCACCGCACCAAAGGCGCGGATTATGCACTTGAGAGCATCCATAATATGAAGGAAGCACTGCCAGAACTCTGGGAAATCACCACTGAGCAGTCTGAAAATGTCCAAACTACACGAGAGATTGCAATTGAGCAACTGGTGGAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_like

Protein Analysis

321

Amino Acids

36.1

Weight (kDa)

4.83

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 58 - 263 3.3e-10 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 809
AccB7I CCANNNNNTGG 1 cut(s) 641
AccII CGCG 2 cut(s) 32, 833
AciI CCGC 5 cut(s) 469, 506, 647, 820, 833
AclWI GGATC 2 cut(s) 129, 425
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 3 cut(s) 222, 487, 710
AfaI GTAC 2 cut(s) 231, 317
AfiI CCNNNNNNNGG 1 cut(s) 641
AflII CTTAAG 1 cut(s) 347
AgsI TTSAA 2 cut(s) 158, 266
AluBI AGCT 3 cut(s) 271, 688, 959
AluI AGCT 3 cut(s) 271, 688, 959
Alw21I GWGCWC 1 cut(s) 519
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 2 cut(s) 129, 425
AoxI GGCC 2 cut(s) 339, 787
ApoI RAATTY 3 cut(s) 222, 487, 710
AspLEI GCGC 2 cut(s) 34, 833
AsuC2I CCSGG 1 cut(s) 665
AsuHPI GGTGA 3 cut(s) 48, 155, 886
AxyI CCTNAGG 1 cut(s) 440
BanI GGYRCC 1 cut(s) 809
BauI CACGAG 1 cut(s) 927
Bbv12I GWGCWC 1 cut(s) 519
BccI CCATC 1 cut(s) 136
BcgI CGANNNNNNTGC 2 cut(s) 664, 698
BciVI GTATCC 1 cut(s) 502
BcnI CCSGG 1 cut(s) 665
BcoDI GTCTC 1 cut(s) 560
BfaI CTAG 2 cut(s) 132, 191
BfrI CTTAAG 1 cut(s) 347
BfuI GTATCC 1 cut(s) 502
Bme1390I CCNGG 1 cut(s) 665
BmiI GGNNCC 2 cut(s) 654, 811
BmrFI CCNGG 1 cut(s) 665
BmsI GCATC 1 cut(s) 861
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 866
BpuMI CCSGG 1 cut(s) 665
BsaJI CCNNGG 1 cut(s) 663
Bsc4I CCNNNNNNNGG 1 cut(s) 641
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 3 cut(s) 22, 646, 954
Bse21I CCTNAGG 1 cut(s) 440
BseDI CCNNGG 1 cut(s) 663
BseGI GGATG 1 cut(s) 852
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMII CTCAG 4 cut(s) 203, 368, 431, 891
BseNI ACTGG 3 cut(s) 22, 646, 954
BsgI GTGCAG 1 cut(s) 627
Bsh1236I CGCG 2 cut(s) 32, 833
BshFI GGCC 2 cut(s) 341, 789
BshNI GGYRCC 1 cut(s) 809
BsiHKAI GWGCWC 1 cut(s) 519
BsiSI CCGG 2 cut(s) 342, 665
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 1 cut(s) 343
BsmI GAATGC 1 cut(s) 756
BsnI GGCC 2 cut(s) 341, 789
Bsp1286I GDGCHC 1 cut(s) 519
Bsp1407I TGTACA 1 cut(s) 315
Bsp143I GATC 3 cut(s) 121, 187, 430
BspACI CCGC 5 cut(s) 469, 506, 647, 820, 833
BspANI GGCC 2 cut(s) 341, 789
BspCNI CTCAG 4 cut(s) 204, 367, 432, 892
BspFNI CGCG 2 cut(s) 32, 833
BspLI GGNNCC 2 cut(s) 654, 811
BspPI GGATC 2 cut(s) 129, 425
BspT107I GGYRCC 1 cut(s) 809
BspTI CTTAAG 1 cut(s) 347
BsrFI RCCGGY 1 cut(s) 341
BsrGI TGTACA 1 cut(s) 315
BsrI ACTGG 3 cut(s) 22, 646, 954
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 663
BssMI GATC 3 cut(s) 121, 187, 430
BssSI CACGAG 1 cut(s) 927
Bst2BI CACGAG 1 cut(s) 927
Bst4CI ACNGT 3 cut(s) 514, 571, 799
BstAFI CTTAAG 1 cut(s) 347
BstAUI TGTACA 1 cut(s) 315
BstC8I GCNNGC 1 cut(s) 690
BstDEI CTNAG 7 cut(s) 212, 240, 354, 440, 455, 900, 960
BstF5I GGATG 1 cut(s) 852
BstFNI CGCG 2 cut(s) 32, 833
BstHHI GCGC 2 cut(s) 34, 833
BstKTI GATC 3 cut(s) 124, 190, 433
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 3 cut(s) 121, 187, 430
BstMWI GCNNNNNNNGC 3 cut(s) 523, 828, 839
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 1 cut(s) 663
BstUI CGCG 2 cut(s) 32, 833
BstX2I RGATCY 1 cut(s) 121
BstXI CCANNNNNNTGG 1 cut(s) 885
BstYI RGATCY 1 cut(s) 121
Bsu36I CCTNAGG 1 cut(s) 440
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 2 cut(s) 341, 789
BtsCI GGATG 1 cut(s) 852
BtsI GCAGTG 2 cut(s) 788, 872
BtsIMutI CAGTG 5 cut(s) 576, 624, 788, 872, 897
Cac8I GCNNGC 1 cut(s) 690
CfoI GCGC 2 cut(s) 34, 833
Cfr10I RCCGGY 1 cut(s) 341
Csp6I GTAC 2 cut(s) 230, 316
CviAII CATG 3 cut(s) 277, 398, 503
CviJI RGCY 9 cut(s) 271, 341, 353, 360, 526, 688, 704, 789, 959
CviKI_1 RGCY 9 cut(s) 271, 341, 353, 360, 526, 688, 704, 789, 959
CviQI GTAC 2 cut(s) 230, 316
DdeI CTNAG 7 cut(s) 212, 240, 354, 440, 455, 900, 960
DpnI GATC 3 cut(s) 123, 189, 432
DpnII GATC 3 cut(s) 121, 187, 430
DraI TTTAAA 1 cut(s) 709
EaeI YGGCCR 1 cut(s) 339
Eco81I CCTNAGG 1 cut(s) 440
EcoRI GAATTC 1 cut(s) 487
FaeI CATG 3 cut(s) 280, 401, 506
FaqI GGGAC 1 cut(s) 343
FatI CATG 3 cut(s) 276, 397, 502
FokI GGATG 1 cut(s) 839
FspBI CTAG 2 cut(s) 132, 191
GlaI GCGC 2 cut(s) 33, 832
HaeIII GGCC 2 cut(s) 341, 789
HapII CCGG 2 cut(s) 342, 665
HhaI GCGC 2 cut(s) 34, 833
Hin1II CATG 3 cut(s) 280, 401, 506
Hin6I GCGC 2 cut(s) 32, 831
HinP1I GCGC 2 cut(s) 32, 831
HindIII AAGCTT 2 cut(s) 269, 957
HinfI GANTC 5 cut(s) 40, 262, 548, 574, 596
HpaII CCGG 2 cut(s) 342, 665
HphI GGTGA 3 cut(s) 48, 155, 886
Hpy188I TCNGA 5 cut(s) 39, 121, 213, 493, 910
Hpy188III TCNNGA 7 cut(s) 132, 191, 219, 296, 428, 439, 484
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 4 cut(s) 54, 138, 188, 859
HpyCH4III ACNGT 3 cut(s) 514, 571, 799
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 9 cut(s) 105, 306, 608, 675, 692, 716, 776, 842, 938
HpyF10VI GCNNNNNNNGC 3 cut(s) 523, 828, 839
HpyF3I CTNAG 7 cut(s) 212, 240, 354, 440, 455, 900, 960
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 3 cut(s) 280, 401, 506
HspAI GCGC 2 cut(s) 32, 831
Kzo9I GATC 3 cut(s) 121, 187, 430
LweI GCATC 1 cut(s) 861
MaeI CTAG 2 cut(s) 132, 191
MaeII ACGT 1 cut(s) 108
MalI GATC 3 cut(s) 123, 189, 432
MboI GATC 3 cut(s) 121, 187, 430
MboII GAAGA 3 cut(s) 623, 710, 736
MfeI CAATTG 1 cut(s) 939
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 519
MluCI AATT 6 cut(s) 222, 299, 477, 487, 710, 939
MlyI GAGTC 2 cut(s) 34, 557
MmeI TCCRAC 1 cut(s) 62
MnlI CCTC 7 cut(s) 219, 396, 435, 529, 738, 800, 823
MseI TTAA 4 cut(s) 26, 249, 348, 708
MslI CAYNNNNRTG 1 cut(s) 168
MspCI CTTAAG 1 cut(s) 347
MspI CCGG 2 cut(s) 342, 665
MspR9I CCNGG 1 cut(s) 665
MunI CAATTG 1 cut(s) 939
Mva1269I GAATGC 1 cut(s) 756
MvnI CGCG 2 cut(s) 32, 833
MwoI GCNNNNNNNGC 3 cut(s) 523, 828, 839
NciI CCSGG 1 cut(s) 665
NdeII GATC 3 cut(s) 121, 187, 430
NlaIII CATG 3 cut(s) 280, 401, 506
NlaIV GGNNCC 2 cut(s) 654, 811
NspI RCATGY 1 cut(s) 506
PctI GAATGC 1 cut(s) 756
PfeI GAWTC 3 cut(s) 262, 574, 596
PflMI CCANNNNNTGG 1 cut(s) 641
PleI GAGTC 2 cut(s) 34, 556
PpsI GAGTC 2 cut(s) 34, 556
PspN4I GGNNCC 2 cut(s) 654, 811
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 2 cut(s) 231, 317
RsaNI GTAC 2 cut(s) 230, 316
RseI CAYNNNNRTG 1 cut(s) 168
SaqAI TTAA 4 cut(s) 26, 249, 348, 708
Sau3AI GATC 3 cut(s) 121, 187, 430
SchI GAGTC 2 cut(s) 34, 557
ScrFI CCNGG 1 cut(s) 665
SduI GDGCHC 1 cut(s) 519
SetI ASST 9 cut(s) 61, 111, 156, 273, 407, 690, 730, 815, 961
SfaNI GCATC 1 cut(s) 861
SmiMI CAYNNNNRTG 1 cut(s) 168
SmlI CTYRAG 2 cut(s) 347, 845
SmoI CTYRAG 2 cut(s) 347, 845
Sse9I AATT 6 cut(s) 222, 299, 477, 487, 710, 939
SsiI CCGC 5 cut(s) 469, 506, 647, 820, 833
SspI AATATT 1 cut(s) 247
SspMI CTAG 2 cut(s) 132, 191
StyD4I CCNGG 1 cut(s) 663
TaaI ACNGT 3 cut(s) 514, 571, 799
TaiI ACGT 1 cut(s) 111
TaqI TCGA 4 cut(s) 369, 684, 723, 747
TaqII GACCGA 1 cut(s) 101
TasI AATT 6 cut(s) 222, 299, 477, 487, 710, 939
TatI WGTACW 2 cut(s) 229, 315
TfiI GAWTC 3 cut(s) 262, 574, 596
Tru1I TTAA 4 cut(s) 26, 249, 348, 708
Tru9I TTAA 4 cut(s) 26, 249, 348, 708
TscAI CASTG 5 cut(s) 576, 631, 788, 879, 904
TspDTI ATGAA 3 cut(s) 374, 386, 878
TspRI CASTG 5 cut(s) 576, 631, 788, 879, 904
Van91I CCANNNNNTGG 1 cut(s) 641
Vha464I CTTAAG 1 cut(s) 347
XapI RAATTY 3 cut(s) 222, 487, 710
XbaI TCTAGA 2 cut(s) 131, 190
XceI RCATGY 1 cut(s) 506
XspI CTAG 2 cut(s) 132, 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.