pycom11g13080
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010356
Physical Location & Seq
Forward (+)
12202837 .. 12206556
3720 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1011 bp
ATGGACAAGAAGGAATTGATTAGCCGAAGAAAGGAAGGGCGGGCGAGGACAGAGAGGGAAATATTGGAAATGTTGGAGCACCCGTTCTTGCCCACGCTCTACGCGAGTATCGACGCGCCCAACTGGTTTTTCCTGCTGACAGAGTTCTGCCCCGGCGGCGACCTCCACGTCCTCCGCCAGCGCCAGCCGTTCAAACGCTTCAACGAACATGCCGTCAGGTTCTACGCTTCGGAGGTGATTGTAGCTTTTGAGTACCTCCACATGATGGGGATTGTGTACCGTGATCTGAAGCCGGAAAACGTTTTAGTTCGATCTGACGGTCACATCATGCTTACGGATTTTGACCTCTCGTTAAAATGCGACGATGGTACATCAACGCCTCAGATCATCTCTACTCAAAATACCCCAAATGCTGCACCACGAAAAGACTACTCACTTGATCCACCCCCATTTGCATCATCTTCATGCATACTGCCCAGTTGTATGGTCCCTTCCGTGTCATGTTTTCACCCGAAAAACAAGCGGAAGAAGAAGCAGAGCCATAAAAATGGCCCCGAGTTTGTGTCCGAGCCCGTTGACGTCCGGTCCATGTCATTTGTCGGGACACACGAGTACTTGGCTCCGGAGATTGTGTCCGGTGAGGGACATGGTAGTGCAGTGGACTGGTGGACGTTAGGGATATTTATTTCTGAACTATTCTATGGTGTGACACCCTTTAGGGGCGTGGACAATGAGCTAACCCTAGCAAATATTGTGGCTCGAGCCCTAGAGCTTCCGAAAGAACCGGCTGTGCCGGCCACGGCCAAGGACCTCATCTCACAACTGCTGGTCAAGGATCCGGCTCGGCGATTGGGATCGTTGATGGGTGCATCGGCAGTCAAGAGGCACCCATTTTTCCAAGGGGTTAATTGGGCGTTGTTGAGATGTACACCTCCACCATTTATTCCCCCACCACTTAGTAGGCGAGATGGATCTGATCAAAGTTGTCCTGAGAAAATGGAATATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

337

Amino Acids

37.92

Weight (kDa)

7.14

Isoelectric Point (pI)

61.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 133 8.9e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 12 - 121 1.4e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 196 - 299 1.8e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 204 - 283 5.3e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
AatII GACGTC 1 cut(s) 582
AccB1I GGYRCC 1 cut(s) 885
AccB7I CCANNNNNTGG 1 cut(s) 265
AccII CGCG 2 cut(s) 104, 116
AccIII TCCGGA 1 cut(s) 622
AciI CCGC 4 cut(s) 40, 156, 175, 523
AclI AACGTT 1 cut(s) 300
AclWI GGATC 5 cut(s) 434, 830, 843, 862, 979
AcoI YGGCCR 2 cut(s) 795, 801
AcuI CTGAAG 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 579
AfaI GTAC 5 cut(s) 254, 278, 370, 614, 928
AfiI CCNNNNNNNGG 3 cut(s) 31, 265, 719
AgsI TTSAA 2 cut(s) 193, 202
AhdI GACNNNNNGTC 1 cut(s) 583
AjiI CACGTC 1 cut(s) 169
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
AloI GAACNNNNNNTCC 2 cut(s) 68, 100
AluBI AGCT 3 cut(s) 245, 736, 772
AluI AGCT 3 cut(s) 245, 736, 772
Alw21I GWGCWC 1 cut(s) 81
AlwI GGATC 5 cut(s) 434, 830, 843, 862, 979
Ama87I CYCGRG 2 cut(s) 554, 759
Aor13HI TCCGGA 1 cut(s) 622
AoxI GGCC 3 cut(s) 550, 795, 801
ApeKI GCWGC 1 cut(s) 413
ArsI GACNNNNNNTTYG 2 cut(s) 198, 230
AspLEI GCGC 2 cut(s) 118, 183
AspS9I GGNCC 4 cut(s) 487, 551, 585, 808
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 3 cut(s) 247, 500, 650
AvaI CYCGRG 2 cut(s) 554, 759
AvaII GGWCC 3 cut(s) 487, 585, 808
BaeI ACNNNNGTAYC 2 cut(s) 91, 124
BamHI GGATCC 1 cut(s) 835
BanI GGYRCC 1 cut(s) 885
BanII GRGCYC 2 cut(s) 573, 766
BarI GAAGNNNNNNTAC 2 cut(s) 475, 507
BauI CACGAG 1 cut(s) 608
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 400
BccI CCATC 4 cut(s) 259, 359, 856, 962
BceAI ACGGC 3 cut(s) 172, 197, 816
BclI TGATCA 1 cut(s) 976
BcnI CCSGG 1 cut(s) 153
BfaI CTAG 2 cut(s) 743, 767
BfoI RGCGCY 1 cut(s) 184
BglI GCCNNNNNGGC 1 cut(s) 156
BisI GCNGC 2 cut(s) 157, 414
BlsI GCNGC 2 cut(s) 158, 415
BmcAI AGTACT 1 cut(s) 614
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 3 cut(s) 487, 585, 808
BmeRI GACNNNNNGTC 1 cut(s) 583
BmeT110I CYCGRG 2 cut(s) 554, 759
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 4 cut(s) 487, 551, 585, 808
BmiI GGNNCC 5 cut(s) 489, 553, 621, 837, 887
BmrFI CCNGG 1 cut(s) 153
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 2 cut(s) 464, 878
BmuI ACTGGG 1 cut(s) 471
BpuMI CCSGG 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 853
BsaHI GRCGYC 1 cut(s) 579
BsaJI CCNNGG 4 cut(s) 151, 798, 804, 898
BsaWI WCCGGW 3 cut(s) 582, 622, 635
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 617, 647
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 265, 719
Bse118I RCCGGY 2 cut(s) 784, 793
Bse1I ACTGG 3 cut(s) 128, 477, 668
Bse8I GATNNNNATC 1 cut(s) 853
BseAI TCCGGA 1 cut(s) 622
BseDI CCNNGG 4 cut(s) 151, 798, 804, 898
BseJI GATNNNNATC 1 cut(s) 853
BseLI CCNNNNNNNGG 3 cut(s) 31, 265, 719
BseMII CTCAG 2 cut(s) 395, 981
BseNI ACTGG 3 cut(s) 128, 477, 668
BseXI GCAGC 1 cut(s) 400
BsgI GTGCAG 2 cut(s) 399, 675
Bsh1236I CGCG 2 cut(s) 104, 116
BshFI GGCC 3 cut(s) 552, 797, 803
BshNI GGYRCC 1 cut(s) 885
BsiHKAI GWGCWC 1 cut(s) 81
BsiHKCI CYCGRG 2 cut(s) 554, 759
BsiSI CCGG 8 cut(s) 153, 293, 583, 623, 636, 785, 794, 839
BslFI GGGAC 3 cut(s) 473, 616, 657
BslI CCNNNNNNNGG 3 cut(s) 31, 265, 719
BsmFI GGGAC 3 cut(s) 473, 616, 657
BsnI GGCC 3 cut(s) 552, 797, 803
BsoBI CYCGRG 2 cut(s) 554, 759
Bsp1286I GDGCHC 3 cut(s) 81, 573, 766
Bsp13I TCCGGA 1 cut(s) 622
Bsp1407I TGTACA 1 cut(s) 926
Bsp143I GATC 8 cut(s) 283, 311, 384, 439, 835, 854, 971, 976
BspACI CCGC 4 cut(s) 40, 156, 175, 523
BspANI GGCC 3 cut(s) 552, 797, 803
BspCNI CTCAG 2 cut(s) 394, 982
BspEI TCCGGA 1 cut(s) 622
BspFNI CGCG 2 cut(s) 104, 116
BspLI GGNNCC 5 cut(s) 489, 553, 621, 837, 887
BspPI GGATC 5 cut(s) 434, 830, 843, 862, 979
BspT107I GGYRCC 1 cut(s) 885
BsrFI RCCGGY 2 cut(s) 784, 793
BsrGI TGTACA 1 cut(s) 926
BsrI ACTGG 3 cut(s) 128, 477, 668
BssAI RCCGGY 2 cut(s) 784, 793
BssECI CCNNGG 4 cut(s) 151, 798, 804, 898
BssMI GATC 8 cut(s) 283, 311, 384, 439, 835, 854, 971, 976
BssNI GRCGYC 1 cut(s) 579
BssSI CACGAG 1 cut(s) 608
BssT1I CCWWGG 2 cut(s) 804, 898
Bst2BI CACGAG 1 cut(s) 608
Bst4CI ACNGT 2 cut(s) 281, 320
BstACI GRCGYC 1 cut(s) 579
BstAUI TGTACA 1 cut(s) 926
BstC8I GCNNGC 4 cut(s) 42, 179, 185, 795
BstDEI CTNAG 3 cut(s) 381, 956, 990
BstDSI CCRYGG 1 cut(s) 798
BstFNI CGCG 2 cut(s) 104, 116
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 2 cut(s) 118, 183
BstKTI GATC 8 cut(s) 286, 314, 387, 442, 838, 857, 974, 979
BstMBI GATC 8 cut(s) 283, 311, 384, 439, 835, 854, 971, 976
BstMWI GCNNNNNNNGC 2 cut(s) 156, 794
BstNSI RCATGY 1 cut(s) 212
BstSCI CCNGG 1 cut(s) 151
BstUI CGCG 2 cut(s) 104, 116
BstV1I GCAGC 1 cut(s) 400
BstX2I RGATCY 2 cut(s) 835, 971
BstXI CCANNNNNNTGG 2 cut(s) 484, 548
BstYI RGATCY 2 cut(s) 835, 971
BsuRI GGCC 3 cut(s) 552, 797, 803
BtgI CCRYGG 1 cut(s) 798
BtrI CACGTC 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 1 cut(s) 663
Cac8I GCNNGC 4 cut(s) 42, 179, 185, 795
CfoI GCGC 2 cut(s) 118, 183
Cfr10I RCCGGY 2 cut(s) 784, 793
Cfr13I GGNCC 4 cut(s) 487, 551, 585, 808
CseI GACGC 1 cut(s) 122
Csp6I GTAC 5 cut(s) 253, 277, 369, 613, 927
CspCI CAANNNNNGTGG 2 cut(s) 735, 770
CviAII CATG 7 cut(s) 209, 262, 328, 465, 501, 589, 647
CviQI GTAC 5 cut(s) 253, 277, 369, 613, 927
DdeI CTNAG 3 cut(s) 381, 956, 990
DpnI GATC 8 cut(s) 285, 313, 386, 441, 837, 856, 973, 978
DpnII GATC 8 cut(s) 283, 311, 384, 439, 835, 854, 971, 976
DrdI GACNNNNNNGTC 1 cut(s) 167
DriI GACNNNNNGTC 1 cut(s) 583
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 2 cut(s) 795, 801
Eam1105I GACNNNNNGTC 1 cut(s) 583
EciI GGCGGA 1 cut(s) 164
Eco130I CCWWGG 2 cut(s) 804, 898
Eco24I GRGCYC 2 cut(s) 573, 766
Eco47I GGWCC 3 cut(s) 487, 585, 808
Eco57I CTGAAG 1 cut(s) 308
Eco88I CYCGRG 2 cut(s) 554, 759
EcoO109I RGGNCCY 1 cut(s) 808
EcoT14I CCWWGG 2 cut(s) 804, 898
EcoT22I ATGCAT 1 cut(s) 470
EcoT38I GRGCYC 2 cut(s) 573, 766
ErhI CCWWGG 2 cut(s) 804, 898
FaeI CATG 7 cut(s) 212, 265, 331, 468, 504, 592, 650
FaqI GGGAC 3 cut(s) 473, 616, 657
FatI CATG 7 cut(s) 208, 261, 327, 464, 500, 588, 646
FauI CCCGC 1 cut(s) 33
FbaI TGATCA 1 cut(s) 976
Fnu4HI GCNGC 2 cut(s) 157, 414
FriOI GRGCYC 2 cut(s) 573, 766
Fsp4HI GCNGC 2 cut(s) 157, 414
FspBI CTAG 2 cut(s) 743, 767
GlaI GCGC 2 cut(s) 117, 182
GluI GCNGC 2 cut(s) 157, 414
HaeII RGCGCY 1 cut(s) 184
HaeIII GGCC 3 cut(s) 552, 797, 803
HapII CCGG 8 cut(s) 153, 293, 583, 623, 636, 785, 794, 839
HgaI GACGC 1 cut(s) 122
HhaI GCGC 2 cut(s) 118, 183
Hin1I GRCGYC 1 cut(s) 579
Hin1II CATG 7 cut(s) 212, 265, 331, 468, 504, 592, 650
Hin6I GCGC 2 cut(s) 116, 181
HinP1I GCGC 2 cut(s) 116, 181
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HpaII CCGG 8 cut(s) 153, 293, 583, 623, 636, 785, 794, 839
HphI GGTGA 3 cut(s) 247, 500, 650
Hpy166II GTNNAC 6 cut(s) 277, 577, 661, 669, 727, 929
Hpy188I TCNGA 8 cut(s) 232, 288, 316, 384, 568, 691, 777, 976
Hpy188III TCNNGA 4 cut(s) 601, 623, 880, 989
Hpy8I GTNNAC 6 cut(s) 277, 577, 661, 669, 727, 929
Hpy99I CGWCG 2 cut(s) 116, 365
HpyAV CCTTC 3 cut(s) 4, 29, 501
HpyCH4III ACNGT 2 cut(s) 281, 320
HpyCH4IV ACGT 4 cut(s) 168, 300, 579, 671
HpyCH4V TGCA 5 cut(s) 416, 455, 468, 656, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 794
HpyF3I CTNAG 3 cut(s) 381, 956, 990
HpySE526I ACGT 4 cut(s) 168, 300, 579, 671
Hsp92I GRCGYC 1 cut(s) 579
Hsp92II CATG 7 cut(s) 212, 265, 331, 468, 504, 592, 650
HspAI GCGC 2 cut(s) 116, 181
Kpn2I TCCGGA 1 cut(s) 622
KroI GCCGGC 1 cut(s) 793
KroNI GCCGGC 1 cut(s) 795
Ksp22I TGATCA 1 cut(s) 976
Kzo9I GATC 8 cut(s) 283, 311, 384, 439, 835, 854, 971, 976
LmnI GCTCC 2 cut(s) 76, 625
Lsp1109I GCAGC 1 cut(s) 400
LweI GCATC 2 cut(s) 464, 878
MaeI CTAG 2 cut(s) 743, 767
MaeII ACGT 4 cut(s) 168, 300, 579, 671
MaeIII GTNAC 2 cut(s) 320, 706
MalI GATC 8 cut(s) 285, 313, 386, 441, 837, 856, 973, 978
MboI GATC 8 cut(s) 283, 311, 384, 439, 835, 854, 971, 976
MboII GAAGA 4 cut(s) 39, 453, 538, 541
MflI RGATCY 2 cut(s) 835, 971
MhlI GDGCHC 3 cut(s) 81, 573, 766
MluCI AATT 2 cut(s) 14, 907
MmeI TCCRAC 1 cut(s) 54
Mph1103I ATGCAT 1 cut(s) 470
MroI TCCGGA 1 cut(s) 622
MroNI GCCGGC 1 cut(s) 793
MseI TTAA 2 cut(s) 353, 906
MslI CAYNNNNRTG 3 cut(s) 463, 546, 651
MspI CCGG 8 cut(s) 153, 293, 583, 623, 636, 785, 794, 839
MspR9I CCNGG 1 cut(s) 153
MvnI CGCG 2 cut(s) 104, 116
MwoI GCNNNNNNNGC 2 cut(s) 156, 794
NaeI GCCGGC 1 cut(s) 795
NciI CCSGG 1 cut(s) 153
NdeII GATC 8 cut(s) 283, 311, 384, 439, 835, 854, 971, 976
NgoMIV GCCGGC 1 cut(s) 793
NlaIII CATG 7 cut(s) 212, 265, 331, 468, 504, 592, 650
NlaIV GGNNCC 5 cut(s) 489, 553, 621, 837, 887
NmeAIII GCCGAG 1 cut(s) 823
NmuCI GTSAC 2 cut(s) 320, 706
NsiI ATGCAT 1 cut(s) 470
NspI RCATGY 1 cut(s) 212
PaeR7I CTCGAG 1 cut(s) 759
PcsI WCGNNNNNNNCGW 4 cut(s) 101, 108, 210, 606
PdiI GCCGGC 1 cut(s) 795
PflMI CCANNNNNTGG 1 cut(s) 265
PkrI GCNGC 2 cut(s) 158, 415
PpuMI RGGWCCY 1 cut(s) 808
Psp1406I AACGTT 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 808
PspN4I GGNNCC 5 cut(s) 489, 553, 621, 837, 887
PspPI GGNCC 4 cut(s) 487, 551, 585, 808
PspPPI RGGWCCY 1 cut(s) 808
PspXI VCTCGAGB 1 cut(s) 759
PsuI RGATCY 2 cut(s) 835, 971
RsaI GTAC 5 cut(s) 254, 278, 370, 614, 928
RsaNI GTAC 5 cut(s) 253, 277, 369, 613, 927
RseI CAYNNNNRTG 3 cut(s) 463, 546, 651
SaqAI TTAA 2 cut(s) 353, 906
SatI GCNGC 2 cut(s) 157, 414
Sau3AI GATC 8 cut(s) 283, 311, 384, 439, 835, 854, 971, 976
Sau96I GGNCC 4 cut(s) 487, 551, 585, 808
ScaI AGTACT 1 cut(s) 614
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 3 cut(s) 81, 573, 766
SfaNI GCATC 2 cut(s) 464, 878
Sfr274I CTCGAG 1 cut(s) 759
SinI GGWCC 3 cut(s) 487, 585, 808
SlaI CTCGAG 1 cut(s) 759
SmiMI CAYNNNNRTG 3 cut(s) 463, 546, 651
SmlI CTYRAG 1 cut(s) 759
SmoI CTYRAG 1 cut(s) 759
Sse9I AATT 2 cut(s) 14, 907
SsiI CCGC 4 cut(s) 40, 156, 175, 523
SspI AATATT 3 cut(s) 63, 751, 1004
SspMI CTAG 2 cut(s) 743, 767
StyD4I CCNGG 1 cut(s) 151
StyI CCWWGG 2 cut(s) 804, 898
TaaI ACNGT 2 cut(s) 281, 320
TaiI ACGT 4 cut(s) 171, 303, 582, 674
TaqI TCGA 3 cut(s) 111, 310, 760
TasI AATT 2 cut(s) 14, 907
TatI WGTACW 2 cut(s) 612, 926
TauI GCSGC 1 cut(s) 159
Tru1I TTAA 2 cut(s) 353, 906
Tru9I TTAA 2 cut(s) 353, 906
TscAI CASTG 1 cut(s) 663
TseFI GTSAC 2 cut(s) 320, 706
TseI GCWGC 1 cut(s) 413
Tsp45I GTSAC 2 cut(s) 320, 706
TspDTI ATGAA 1 cut(s) 453
TspGWI ACGGA 2 cut(s) 350, 484
TspRI CASTG 1 cut(s) 663
Van91I CCANNNNNTGG 1 cut(s) 265
VpaK11BI GGWCC 3 cut(s) 487, 585, 808
XceI RCATGY 1 cut(s) 212
XhoI CTCGAG 1 cut(s) 759
XspI CTAG 2 cut(s) 743, 767
ZraI GACGTC 1 cut(s) 580
ZrmI AGTACT 1 cut(s) 614
Zsp2I ATGCAT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.