pycom11g16330

chromodomain-helicase-DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0007957
Physical Location & Seq
Reverse (-)
17911569 .. 17929967
18399 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5076 bp
ATGGATGTGCAGTATCAAAGTGAAGGCGAACATGACAATGCTAGTAGGTTGCACAATGAGGTAGCAGCAGATGAGGGTATGGGTACAAGAGTTTCCAATTTGCCCTCTTCTGGGAGGAGGACAACTGTGGCTGGAAAGTGGGGTTCGACCTTTTGGAAGGACTGTCAGCCTATGCACTCACAGGGTGGGTCTGATTCTGGTCAGGAAACTAAAAGTGGGTCGGACCACAGAAATGCTGTTGGATCTGAAGATAATTCTTCAGATGTTGGGGAAGATAGAATAGATTTTGAACATGATGGCAGGCCAAAAGTAAGCAAGGGACAACAGGGCCATTCTGACATCCCTGCAGATGAAATGCTATCTGATGAATATTATGAGCAGGATGGGGAAGAACAAAGTGAATCGATGCACTATAGAGGGTTTCATCATTCCGTTGGGTCAAATTCTAGGCCACAATCAAAGCCTAATTCAGTCAGTAACCATGCAGATCATAGAACTTCTAGAAATGATATTGAAGATTTTGAAGATGGTGATGATGCCAACGAAGATCTTGATGCTGCTGATGCTGATTATGAAGAGGACGAAGATGAAGATGATCCTGATGATGCAGATTTTGAACCTGACTATGGTGTTGGGAGTGGTCGCACTGCACAGAAGGATAAAAATTGGAATGGTGAAGATTCTGATGAAGATAATGATAGTGATGATGAGCTTGATGTTTCAGATGAAGATGATTCTTATTATGGAAAGAAACCTAAGAGGAGGCATCATGGTAATGCTAAATCGACGAGGGAACGCAAATCATATCATTCATCTAGTCGCCAAAGAAGAGGGAAGTCATCATTTGATGATGATGAGTCCTCAGCGGAGGACTCTGAGAGTGATGGTGACGAGGATTTTAAAGGTATCAAAAGGAAAGGTGTACATACCCGCAAAAGTAACGGCCGAAGAAATGTTCTTGGACAAAATGGTGAGGTGCGAACTTCTACTAGGTCTGTTAGGAAGGTCTCATATGTTGAAAGTGATGGAAGCGATGAAGTGGAAGAAGGAAAGAAAAAGAAGTCCCAGAAGGAGGAGATTGAAGAAGAGGATGGTGACTATATTGAAAAGGTGCTCTGGCATCAGCCTAAAGGCATGGCTGAAGATGCTCTCAGGAACAATAGATCAACGGATCCTATCCTATTAAGTCACCTGTTTGATTCTGAGCCAGATTGGAATATTATGGAGTTCTTGATTAAATGGAAAGGGCAGTCCCATTTGCACTGTCAGTGGAAATCAATTTCCGAGCTACAAAATCTTAGCGGTTTTAAGAAAGTTTTAAATTACACTAAAAAGGTGATGGAAGATGCCAGGTACAGGAACACAATATCACGTGAGGAGATTGAGGTTCATGATGTGAGCAAGGAAATGGACTTAGATCTCATCAAGCAGAATAGTCAGGTGGAGAGAATAATTGCAGACCGCATCAGGCAAGATAGCTCTGGTGATGTGGGTCAGGAATATCTAGTCAAGTGGCAAGGGTTGTCTTATGCTGAAGCAACATGGGAGAAGGATGTAGATATTGGGTTTGCACAAGATGCTATCGATGAGTTCAAGGCTCGTGAAGCTGCAATGGCTGTACAAGGAAAAATGGTGGATCTCCAGCGCAAGAGGAGCAAAGGAAGTCTACGGAAGCTCGAGGAACAGCCGGAATGGTTGAAGGGAGGAAGTCTTCGTGATTATCAGCTTGAGGGTTTGAATTTTCTTGTTAATAGTTGGAGGAATGATACCAATGTCATTTTAGCTGATGAAATGGGTCTTGGTAAAACTGTTCAATCAGTTTCAATGCTTGGTTTTTTACAGAATGCTCAACAGATCAACGAGCCATTTCTTGTTGTTGTACCATTATCCACTTTGTCTAACTGGGCCAAAGAATTTAGGAAGTGGCTCCCTGATATGAATATTATTGTGTATGTTGGTACTCGTGCAAGCCGAGAGGTTTGTCAACAGTATGAATTTAATAATGATAAAATAGGAAGCCGGCCTATAAAATTCAATGCACTATTGACGACATATGAGGTGGTTTTAAAGGATAAGGCTGTTCTTTCCAAGATCAAGTGGAATTATCTAATGGTTGATGAAGCTCATAGATTAAAGAACAGTGAGGCACAGTTGTACACAACCCTCTTGGAATTTAGCACGAAGAACAAGTTGCTTATAACTGGTACTCCCTTGCAGAACAGCGTAGAGGAGTTATGGGCTCTGCTTCACTTCCTCGACCCTGATAAGTTCAAGAATAAAGATGATTTTGTTCAGAATTACAAGAATCTCAGTTCATTCAATGAGATTGAGCTTGCTAATCTGCATATGGAATTGAGGCCTCACATTCTTCGAAGAGTTATCAAGGATGTGGAAAAGTCTTTACCTCCTAAAATTGAACGAATTCTTAGGGTTGAAATGTCTCCTCTTCAGAAACAGTACTATAAGTGGATTTTGGAACGCAACTTTCATAACTTGAACAAAGGAGTTCGTGGGAATCAGGTTTCGTTGTTGAATATTGTGGTAGAGTTGAAGAAATGTTGTAATCATCCCTTTCTGTTTGAAAGTGCTGACCATGGTTATGGTGGGGACTCAAGTAGTAAAGATGGCAGTAAATTGGAGAGGATTATTTTAAGCAGCGGAAAGTTGGTTATACTTGATAAGCTTCTTATGAAATTGCATGAAACAAAGCACCGTGTTCTCATATTTTCCCAGATGGTAAGAATGTTGGACATACTGGCGGAGTATATGTCAATTAGAGGTTTTCAATTTCAGCGGCTTGATGGGAGTACAAAGGCCGATTTACGCCACCAAGCAATGGAACATTTTAATGCGCCTGGTAGTGAAGATTTTTGCTTCCTCCTTTCAACTCGAGCAGGTGGCCTTGGTATCAACCTTGCCACAGCGGATACAGTAATAATATTCGACTCAGATTGGAATCCCCAAAATGACCTTCAGGCAATGAGCAGAGCGCACAGAATAGGGCAACAAGAAGTTGTTAACATCTATAGATTTGTCACAAGCAAAAGTGTCGAGGAAGATATTCTGGAGAGAGCTAAGAAAAAGATGGTGCTTGACCATTTGGTGATTCAAAAATTAAATGCAGAGGGTAGGTTGGAGAAGAAAGAATCTAAGAAGGGGACTTTATTTGACAAAAATGAGCTGTCTGCTATTCTACGATTTGGGGCTGAGGAGCTTTTCAAGGAAGACAAAAATGATGAAGAGAGCAAGAAAGGACTCTTGAGTATGGATATTGATGAGATTCTTGAAAGAGCAGAAAAGGTTGAAGAGAAGGAAACTGCAGAGGAAGGGAATGAATTGTTGAGTGCTTTTAAGGTTGCAAATTTTGGTACTGGTGAAGATGATGGTAGTTTTTGGAGTCGTTGGATAAAGCCAGAAGCAGTATCCCAAGCCGAAGAAGCACTTGCTCCTCGTGCTACAAGGAATACTAAGAGCTATGCAGAAGCCGATCAGCCTGATAGAAGTAATAAAAGAAAAAAGAAAGAATCTGAGCCTCAGGAAAGAGTCCAGAAAAGGCGAAAATCAGATTACACAGTTACCTCAGCACCAATGATTGGCGGAGCATCGGCTCAAGTTAGGAGATGGTCTTCTGGGAACTTATCAAAGAGAGATGCATTGCGATTTTCTCGTGCTGTTATGAAATTTGGGAATGAGAGCCAAATTGCCTCGATAGTTGGGGAGGTTGGTGGTGCCGTTGCAGCAGCTTCAACTGAAGCACAGGTTGAGCTTTTTAATGCATTGATTGATGGCTGCACAGAAGCAGTGGAAGTGGGGAGTCTGGACCCGAAGGGGCCCTTGCTGGATTTTTTTGGTGTCCCTGTCAAAGCTGGTGATCTGCTCAATCGTGTTCGTGAACTTCAGTACCTGGCAAAACGCATTAGTCGATATGACGATCCCATTGCTCAGTTTCGTGTCTTGATGTACCTCAAGCCTTCAAATTGGTCGAAAGGTTGTGGTTGGAATCAAATTGATGATGCAAGATTGCTTATTGGAATATACTACCATGGCTTTGGAAATTGGGAAAAGATACGTTTAGATGAAAGGCTTGGCCTTACAAAGAAAATTGCTCCAGTGGAACTTCAACACCATGAAACATTTTTACCCCGTGCTCCAAATTTGAGAGATCGTGCTAACGCTCTTTTGGAAATGGAAATTGCAGCCCTTGGTGGAAAGAATGCAAATGCTAAAGTGGGTCGTAAGTCTTCAAGGGATAGGGAAACTACTGTCAATGTTTCTTTAGCTCGCAGTGGGCTAAAGAAAGGAAAAACTGGTTCTTCTAGAGTTAATGTTCAAATGAACAAGGGTAGACCACTGAAACCCCAGAAAGTTGAACCTTTAGTGAAAGAGGAGGGTGAAATGTCGGACGATGATGAAGTGTATGAGAAGTTCAAGGAGGAGAAATGGATGGAATGGTGTGAAGAGATGATGAAGGATGAAACTAAAACCTTGAAAAGTCTTGAACGGTTGCAGACAATTAGTGCCAATCTTCCAAAGGAAAAGGTGCTTTCCAAGATTCGAAGTTATCTACAACTTCTTGGACGAACGATAGATCAAATTGTTTTAGAAAATGAGGAGGAACCGTATGGACAAGATAGGATGACCATGCGCTTGTGGAAATATGTTTCTACCTTTTCAAATCTGTCCGGGGAGAGACTTCATCAAATATATTCAAAACTTAAACAGCACCAGGATGTGGAGGCTGGCCCTTCCCACATGAACGGCTCCGCTTCTGGCCCCTTCGGCAGAGACAGTGACCCTTCTCCCTTCTCCTTCCCTTCGGACAGGCAAAGAGGTCATGATACAGCCAAGTTTGAAGCATGGAAACGGAGGAGGAGAGGGGAAGCTGATAGTAATTTCCAAGTTCAGGCCCCATCTCAAAGAAATATGAATAATGGTACCCGGTTAACTGATCCAAGCTCATTAGGGATTCTTGGTGCAGGACCATCAGAAAATAAACGCACTGTGAATGAAAGGCCTTACAGGACGCGGCAGGCAGGTTTACCAACAAAACAAGGTTTTACATCACGTATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Chromo DUF4208 Helicase_C SLIDE SNF2_N

Protein Analysis

1692

Amino Acids

192.35

Weight (kDa)

5.77

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromo PF00385 480 - 534 1.7e-14 Chromo (CHRromatin Organisation MOdifier) domain
ResIII PF04851 570 - 737 1.4e-11 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 574 - 858 1.5e-85 SNF2-related domain
DEAD PF00270 585 - 742 1.8e-06 DEAD/DEAH box helicase
HDA2-3 PF11496 809 - 1033 2.7e-11 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 888 - 1001 6.1e-26 Helicase conserved C-terminal domain
HTH_CHD1_Hrp3 PF23588 1312 - 1394 1.9e-23 ATP-dependent helicase CHD1-2/hrp3 HTH domain
CHD1-like_C PF13907 1477 - 1571 4e-15 Chromodomain-helicase-DNA-binding protein 1-like, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2198
AarI CACCTGC 1 cut(s) 2885
Acc36I ACCTGC 2 cut(s) 2885, 5024
Acc65I GGTACC 1 cut(s) 4934
AccB1I GGYRCC 2 cut(s) 3728, 4934
AccB7I CCANNNNNTGG 3 cut(s) 2836, 3593, 4732
AccI GTMKAC 2 cut(s) 1666, 4345
AccII CGCG 1 cut(s) 5026
AclWI GGATC 7 cut(s) 250, 590, 1166, 1179, 1644, 3926, 4943
AcoI YGGCCR 1 cut(s) 943
AcuI CTGAAG 8 cut(s) 243, 267, 1161, 1554, 2432, 2957, 3771, 3881
AcvI CACGTG 1 cut(s) 1373
AdeI CACNNNGTG 1 cut(s) 185
AfiI CCNNNNNNNGG 9 cut(s) 110, 111, 626, 1072, 1356, 2836, 3593, 4732, 4903
AjnI CCWGG 4 cut(s) 1349, 2854, 3903, 4725
AjuI GAANNNNNNNTTGG 4 cut(s) 1782, 1814, 2955, 2987
Alw21I GWGCWC 2 cut(s) 1116, 4150
Alw26I GTCTC 4 cut(s) 1012, 2445, 4684, 4779
AlwI GGATC 7 cut(s) 250, 590, 1166, 1179, 1644, 3926, 4943
AlwNI CAGNNNCTG 1 cut(s) 3904
Ama87I CYCGRG 2 cut(s) 1676, 2889
ApaI GGGCCC 1 cut(s) 3834
ApeKI GCWGC 8 cut(s) 65, 557, 1607, 2655, 3737, 3740, 3789, 4196
Asp700I GAANNNNTTC 2 cut(s) 2421, 3060
Asp718I GGTACC 1 cut(s) 4934
AspLEI GCGC 4 cut(s) 1647, 2854, 2992, 4647
AsuC2I CCSGG 2 cut(s) 4684, 4939
AsuII TTCGAA 2 cut(s) 2371, 4555
AvaI CYCGRG 2 cut(s) 1676, 2889
AvaII GGWCC 3 cut(s) 223, 3820, 4979
AxyI CCTNAGG 1 cut(s) 3534
BaeGI GKGCMC 1 cut(s) 3834
BaeI ACNNNNGTAYC 6 cut(s) 75, 108, 1758, 1791, 3884, 3917
BamHI GGATCC 1 cut(s) 1171
BanI GGYRCC 2 cut(s) 3728, 4934
BanII GRGCYC 2 cut(s) 2242, 3834
BauI CACGAG 4 cut(s) 1599, 1962, 3450, 3666
BbrPI CACGTG 1 cut(s) 1373
BbsI GAAGAC 4 cut(s) 1703, 3231, 3618, 4233
Bbv12I GWGCWC 2 cut(s) 1116, 4150
BbvCI CCTCAGC 3 cut(s) 862, 3207, 3580
BbvI GCAGC 8 cut(s) 77, 544, 1594, 2667, 3749, 3752, 3776, 4208
BceAI ACGGC 3 cut(s) 958, 3716, 4774
BcgI CGANNNNNNTGC 4 cut(s) 3031, 3065, 3920, 3954
BciT130I CCWGG 4 cut(s) 1351, 2856, 3905, 4727
BciVI GTATCC 2 cut(s) 2920, 3433
BcnI CCSGG 2 cut(s) 4684, 4939
BcoDI GTCTC 4 cut(s) 1012, 2445, 4684, 4779
BfaI CTAG 7 cut(s) 42, 447, 501, 816, 990, 1505, 4317
BfmI CTRYAG 4 cut(s) 345, 412, 3025, 3318
BfuAI ACCTGC 2 cut(s) 2885, 5024
BfuI GTATCC 2 cut(s) 2920, 3433
BglI GCCNNNNNGGC 1 cut(s) 4779
BglII AGATCT 2 cut(s) 547, 1417
BmcAI AGTACT 1 cut(s) 2459
Bme1390I CCNGG 6 cut(s) 1351, 2856, 3905, 4684, 4727, 4939
Bme18I GGWCC 3 cut(s) 223, 3820, 4979
BmeT110I CYCGRG 2 cut(s) 1676, 2889
BmrFI CCNGG 6 cut(s) 1351, 2856, 3905, 4684, 4727, 4939
BmrI ACTGGG 1 cut(s) 1912
BmuI ACTGGG 1 cut(s) 1912
BpiI GAAGAC 4 cut(s) 1703, 3231, 3618, 4233
BpmI CTGGAG 3 cut(s) 1625, 3086, 4092
Bpu10I CCTNAGC 3 cut(s) 862, 3207, 3580
Bpu14I TTCGAA 2 cut(s) 2371, 4555
BpuEI CTTGAG 5 cut(s) 1748, 2596, 3280, 3594, 3950
BpuMI CCSGG 2 cut(s) 4684, 4939
Bsa29I ATCGAT 2 cut(s) 404, 1584
BsaAI YACGTR 2 cut(s) 1373, 5066
BsaBI GATNNNNATC 1 cut(s) 2955
BsaI GGTCTC 1 cut(s) 1012
BsaJI CCNNGG 5 cut(s) 2593, 2902, 4042, 4201, 4683
BsaXI ACNNNNNCTCC 6 cut(s) 2191, 2221, 2753, 2783, 4604, 4634
Bsc4I CCNNNNNNNGG 9 cut(s) 110, 111, 626, 1072, 1356, 2836, 3593, 4732, 4903
Bse118I RCCGGY 1 cut(s) 2019
Bse1I ACTGG 6 cut(s) 1907, 2206, 2760, 3376, 4109, 4312
Bse21I CCTNAGG 1 cut(s) 3534
Bse3DI GCAATG 5 cut(s) 1617, 2841, 2985, 3653, 3936
Bse8I GATNNNNATC 1 cut(s) 2955
BseBI CCWGG 4 cut(s) 1351, 2856, 3905, 4727
BseCI ATCGAT 2 cut(s) 404, 1584
BseDI CCNNGG 5 cut(s) 2593, 2902, 4042, 4201, 4683
BseJI GATNNNNATC 1 cut(s) 2955
BseLI CCNNNNNNNGG 9 cut(s) 110, 111, 626, 1072, 1356, 2836, 3593, 4732, 4903
BseMI GCAATG 5 cut(s) 1617, 2841, 2985, 3653, 3936
BseNI ACTGG 6 cut(s) 1907, 2206, 2760, 3376, 4109, 4312
BseSI GKGCMC 1 cut(s) 3834
BseX3I CGGCCG 1 cut(s) 943
BseXI GCAGC 8 cut(s) 77, 544, 1594, 2667, 3749, 3752, 3776, 4208
BsgI GTGCAG 4 cut(s) 29, 633, 3775, 4995
Bsh1236I CGCG 1 cut(s) 5026
Bsh1285I CGRYCG 1 cut(s) 946
BshNI GGYRCC 2 cut(s) 3728, 4934
BshVI ATCGAT 2 cut(s) 404, 1584
BsiEI CGRYCG 1 cut(s) 946
BsiHKAI GWGCWC 2 cut(s) 1116, 4150
BsiHKCI CYCGRG 2 cut(s) 1676, 2889
BsiSI CCGG 4 cut(s) 1688, 2020, 4683, 4939
BslFI GGGAC 6 cut(s) 333, 1048, 1237, 2621, 3172, 3839
BslI CCNNNNNNNGG 9 cut(s) 110, 111, 626, 1072, 1356, 2836, 3593, 4732, 4903
BsmAI GTCTC 4 cut(s) 1012, 2445, 4684, 4779
BsmFI GGGAC 6 cut(s) 333, 1048, 1237, 2621, 3172, 3839
BsmI GAATGC 2 cut(s) 1849, 4219
Bso31I GGTCTC 1 cut(s) 1012
BsoBI CYCGRG 2 cut(s) 1676, 2889
Bsp119I TTCGAA 2 cut(s) 2371, 4555
Bsp120I GGGCCC 1 cut(s) 3830
Bsp1286I GDGCHC 4 cut(s) 1116, 2242, 3834, 4150
Bsp1407I TGTACA 3 cut(s) 922, 1618, 2154
Bsp19I CCATGG 2 cut(s) 2593, 4042
BspDI ATCGAT 2 cut(s) 404, 1584
BspFNI CGCG 1 cut(s) 5026
BspHI TCATGA 2 cut(s) 1390, 4834
BspMAI CTGCAG 2 cut(s) 349, 3322
BspMI ACCTGC 2 cut(s) 2885, 5024
BspPI GGATC 7 cut(s) 250, 590, 1166, 1179, 1644, 3926, 4943
BspT104I TTCGAA 2 cut(s) 2371, 4555
BspT107I GGYRCC 2 cut(s) 3728, 4934
BspTNI GGTCTC 1 cut(s) 1012
BsrDI GCAATG 5 cut(s) 1617, 2841, 2985, 3653, 3936
BsrFI RCCGGY 1 cut(s) 2019
BsrGI TGTACA 3 cut(s) 922, 1618, 2154
BsrI ACTGG 6 cut(s) 1907, 2206, 2760, 3376, 4109, 4312
BssAI RCCGGY 1 cut(s) 2019
BssECI CCNNGG 5 cut(s) 2593, 2902, 4042, 4201, 4683
BssSI CACGAG 4 cut(s) 1599, 1962, 3450, 3666
BssT1I CCWWGG 4 cut(s) 2593, 2902, 4042, 4201
Bst2BI CACGAG 4 cut(s) 1599, 1962, 3450, 3666
Bst2UI CCWGG 4 cut(s) 1351, 2856, 3905, 4727
Bst6I CTCTTC 9 cut(s) 112, 570, 823, 1080, 2368, 2451, 3234, 3300, 4452
BstAPI GCANNNNNTGC 1 cut(s) 1577
BstAUI TGTACA 3 cut(s) 922, 1618, 2154
BstBAI YACGTR 2 cut(s) 1373, 5066
BstBI TTCGAA 2 cut(s) 2371, 4555
BstC8I GCNNGC 7 cut(s) 302, 1969, 2021, 2334, 4282, 4741, 5031
BstDSI CCRYGG 2 cut(s) 2593, 4042
BstFNI CGCG 1 cut(s) 5026
BstHHI GCGC 4 cut(s) 1647, 2854, 2992, 4647
BstMAI GTCTC 4 cut(s) 1012, 2445, 4684, 4779
BstMCI CGRYCG 1 cut(s) 946
BstNI CCWGG 4 cut(s) 1351, 2856, 3905, 4727
BstSCI CCNGG 6 cut(s) 1349, 2854, 3903, 4682, 4725, 4937
BstSFI CTRYAG 4 cut(s) 345, 412, 3025, 3318
BstSLI GKGCMC 1 cut(s) 3834
BstUI CGCG 1 cut(s) 5026
BstV1I GCAGC 8 cut(s) 77, 544, 1594, 2667, 3749, 3752, 3776, 4208
BstV2I GAAGAC 4 cut(s) 1703, 3231, 3618, 4233
BstX2I RGATCY 5 cut(s) 242, 547, 1171, 1417, 1636
BstXI CCANNNNNNTGG 2 cut(s) 2600, 4049
BstYI RGATCY 5 cut(s) 242, 547, 1171, 1417, 1636
BstZI CGGCCG 1 cut(s) 943
Bsu15I ATCGAT 2 cut(s) 404, 1584
Bsu36I CCTNAGG 1 cut(s) 3534
BsuI GTATCC 2 cut(s) 2920, 3433
BsuTUI ATCGAT 2 cut(s) 404, 1584
BtgI CCRYGG 2 cut(s) 2593, 4042
BtgZI GCGATG 1 cut(s) 1047
BtsI GCAGTG 3 cut(s) 645, 3807, 4291
BveI ACCTGC 2 cut(s) 2885, 5024
Cac8I GCNNGC 7 cut(s) 302, 1969, 2021, 2334, 4282, 4741, 5031
CaiI CAGNNNCTG 1 cut(s) 3904
CciI TCATGA 2 cut(s) 1390, 4834
CfoI GCGC 4 cut(s) 1647, 2854, 2992, 4647
Cfr10I RCCGGY 1 cut(s) 2019
ClaI ATCGAT 2 cut(s) 404, 1584
CseI GACGC 1 cut(s) 5032
DraI TTTAAA 3 cut(s) 901, 1320, 2067
DraIII CACNNNGTG 1 cut(s) 185
EaeI YGGCCR 1 cut(s) 943
EagI CGGCCG 1 cut(s) 943
Eam1104I CTCTTC 9 cut(s) 112, 570, 823, 1080, 2368, 2451, 3234, 3300, 4452
EarI CTCTTC 9 cut(s) 112, 570, 823, 1080, 2368, 2451, 3234, 3300, 4452
EciI GGCGGA 2 cut(s) 2774, 3612
EclXI CGGCCG 1 cut(s) 943
Eco130I CCWWGG 4 cut(s) 2593, 2902, 4042, 4201
Eco147I AGGCCT 2 cut(s) 2359, 5014
Eco24I GRGCYC 2 cut(s) 2242, 3834
Eco31I GGTCTC 1 cut(s) 1012
Eco47I GGWCC 3 cut(s) 223, 3820, 4979
Eco52I CGGCCG 1 cut(s) 943
Eco57I CTGAAG 8 cut(s) 243, 267, 1161, 1554, 2432, 2957, 3771, 3881
Eco72I CACGTG 1 cut(s) 1373
Eco81I CCTNAGG 1 cut(s) 3534
Eco88I CYCGRG 2 cut(s) 1676, 2889
EcoO109I RGGNCCY 3 cut(s) 3830, 3831, 4906
EcoRI GAATTC 1 cut(s) 2421
EcoRII CCWGG 4 cut(s) 1349, 2854, 3903, 4725
EcoT14I CCWWGG 4 cut(s) 2593, 2902, 4042, 4201
EcoT22I ATGCAT 2 cut(s) 3655, 3778
EcoT38I GRGCYC 2 cut(s) 2242, 3834
ErhI CCWWGG 4 cut(s) 2593, 2902, 4042, 4201
FalI AAGNNNNNCTT 4 cut(s) 3426, 3458, 3818, 3850
FaqI GGGAC 6 cut(s) 333, 1048, 1237, 2621, 3172, 3839
FauI CCCGC 1 cut(s) 938
FauNDI CATATG 3 cut(s) 1012, 2053, 2346
FblI GTMKAC 2 cut(s) 1666, 4345
FriOI GRGCYC 2 cut(s) 2242, 3834
FspBI CTAG 7 cut(s) 42, 447, 501, 816, 990, 1505, 4317
GlaI GCGC 4 cut(s) 1646, 2853, 2991, 4646
GsuI CTGGAG 3 cut(s) 1625, 3086, 4092
HapII CCGG 4 cut(s) 1688, 2020, 4683, 4939
HgaI GACGC 1 cut(s) 5032
HhaI GCGC 4 cut(s) 1647, 2854, 2992, 4647
Hin6I GCGC 4 cut(s) 1645, 2852, 2990, 4645
HinP1I GCGC 4 cut(s) 1645, 2852, 2990, 4645
HincII GTYRAC 3 cut(s) 1985, 3019, 4944
HindII GTYRAC 3 cut(s) 1985, 3019, 4944
HindIII AAGCTT 1 cut(s) 2681
HpaI GTTAAC 2 cut(s) 3019, 4944
HpaII CCGG 4 cut(s) 1688, 2020, 4683, 4939
Hpy166II GTNNAC 9 cut(s) 923, 1667, 1985, 2157, 3019, 3893, 4346, 4944, 5039
Hpy8I GTNNAC 9 cut(s) 923, 1667, 1985, 2157, 3019, 3893, 4346, 4944, 5039
Hpy99I CGWCG 1 cut(s) 790
HpyCH4IV ACGT 3 cut(s) 1372, 4069, 5065
HpySE526I ACGT 3 cut(s) 1372, 4069, 5065
HspAI GCGC 4 cut(s) 1645, 2852, 2990, 4645
KpnI GGTACC 1 cut(s) 4938
KroI GCCGGC 1 cut(s) 2019
KroNI GCCGGC 1 cut(s) 2021
KspAI GTTAAC 2 cut(s) 3019, 4944
LmnI GCTCC 8 cut(s) 1653, 1932, 3211, 3451, 3599, 4111, 4153, 4766
Lsp1109I GCAGC 8 cut(s) 77, 544, 1594, 2667, 3749, 3752, 3776, 4208
MaeI CTAG 7 cut(s) 42, 447, 501, 816, 990, 1505, 4317
MaeII ACGT 3 cut(s) 1372, 4069, 5065
MaeIII GTNAC 8 cut(s) 476, 887, 938, 1094, 1187, 3034, 3574, 4790
MflI RGATCY 5 cut(s) 242, 547, 1171, 1417, 1636
MhlI GDGCHC 4 cut(s) 1116, 2242, 3834, 4150
MlyI GAGTC 8 cut(s) 866, 866, 2603, 2939, 3249, 3406, 3552, 3823
MmeI TCCRAC 8 cut(s) 201, 220, 1736, 2727, 3114, 3383, 3977, 4380
Mph1103I ATGCAT 2 cut(s) 3655, 3778
MroNI GCCGGC 1 cut(s) 2019
MroXI GAANNNNTTC 2 cut(s) 2421, 3060
MslI CAYNNNNRTG 6 cut(s) 36, 231, 774, 2598, 2847, 4728
MspA1I CMGCKG 4 cut(s) 866, 2658, 2794, 2924
MspI CCGG 4 cut(s) 1688, 2020, 4683, 4939
MspR9I CCNGG 6 cut(s) 1351, 2856, 3905, 4684, 4727, 4939
Mva1269I GAATGC 2 cut(s) 1849, 4219
MvaI CCWGG 4 cut(s) 1351, 2856, 3905, 4727
MvnI CGCG 1 cut(s) 5026
NaeI GCCGGC 1 cut(s) 2021
NciI CCSGG 2 cut(s) 4684, 4939
NcoI CCATGG 2 cut(s) 2593, 4042
NdeI CATATG 3 cut(s) 1012, 2053, 2346
NgoMIV GCCGGC 1 cut(s) 2019
NmeAIII GCCGAG 1 cut(s) 1997
NmuCI GTSAC 5 cut(s) 887, 1094, 1187, 3034, 4790
NsiI ATGCAT 2 cut(s) 3655, 3778
NspV TTCGAA 2 cut(s) 2371, 4555
PaeR7I CTCGAG 2 cut(s) 1676, 2889
PagI TCATGA 2 cut(s) 1390, 4834
PaqCI CACCTGC 1 cut(s) 2885
PceI AGGCCT 2 cut(s) 2359, 5014
PcsI WCGNNNNNNNCGW 2 cut(s) 1969, 3919
PctI GAATGC 2 cut(s) 1849, 4219
PdiI GCCGGC 1 cut(s) 2021
PdmI GAANNNNTTC 2 cut(s) 2421, 3060
PflMI CCANNNNNTGG 3 cut(s) 2836, 3593, 4732
PleI GAGTC 8 cut(s) 865, 866, 2603, 2939, 3249, 3405, 3551, 3822
PmaCI CACGTG 1 cut(s) 1373
PmlI CACGTG 1 cut(s) 1373
PpsI GAGTC 8 cut(s) 865, 866, 2603, 2939, 3249, 3405, 3551, 3822
Ppu21I YACGTR 2 cut(s) 1373, 5066
PsiI TTATAA 1 cut(s) 2198
Psp6I CCWGG 4 cut(s) 1349, 2854, 3903, 4725
PspCI CACGTG 1 cut(s) 1373
PspGI CCWGG 4 cut(s) 1349, 2854, 3903, 4725
PspOMI GGGCCC 1 cut(s) 3830
PspXI VCTCGAGB 2 cut(s) 1676, 2889
PsrI GAACNNNNNNTAC 2 cut(s) 4409, 4441
PstI CTGCAG 2 cut(s) 349, 3322
PstNI CAGNNNCTG 1 cut(s) 3904
PsuI RGATCY 5 cut(s) 242, 547, 1171, 1417, 1636
RseI CAYNNNNRTG 6 cut(s) 36, 231, 774, 2598, 2847, 4728
ScaI AGTACT 1 cut(s) 2459
SchI GAGTC 8 cut(s) 866, 866, 2603, 2939, 3249, 3406, 3552, 3823
ScrFI CCNGG 6 cut(s) 1351, 2856, 3905, 4684, 4727, 4939
SduI GDGCHC 4 cut(s) 1116, 2242, 3834, 4150
SfcI CTRYAG 4 cut(s) 345, 412, 3025, 3318
Sfr274I CTCGAG 2 cut(s) 1676, 2889
SfuI TTCGAA 2 cut(s) 2371, 4555
SinI GGWCC 3 cut(s) 223, 3820, 4979
SlaI CTCGAG 2 cut(s) 1676, 2889
SmiMI CAYNNNNRTG 6 cut(s) 36, 231, 774, 2598, 2847, 4728
SmlI CTYRAG 7 cut(s) 1676, 1727, 2611, 2889, 3259, 3609, 3965
SmoI CTYRAG 7 cut(s) 1676, 1727, 2611, 2889, 3259, 3609, 3965
SseBI AGGCCT 2 cut(s) 2359, 5014
SspI AATATT 5 cut(s) 371, 1219, 1942, 2536, 2940
SspMI CTAG 7 cut(s) 42, 447, 501, 816, 990, 1505, 4317
StuI AGGCCT 2 cut(s) 2359, 5014
StyD4I CCNGG 6 cut(s) 1349, 2854, 3903, 4682, 4725, 4937
StyI CCWWGG 4 cut(s) 2593, 2902, 4042, 4201
TaiI ACGT 3 cut(s) 1375, 4072, 5068
TatI WGTACW 5 cut(s) 922, 1618, 2154, 2457, 2807
TauI GCSGC 2 cut(s) 2797, 5029
TseFI GTSAC 5 cut(s) 887, 1094, 1187, 3034, 4790
TseI GCWGC 8 cut(s) 65, 557, 1607, 2655, 3737, 3740, 3789, 4196
Tsp45I GTSAC 5 cut(s) 887, 1094, 1187, 3034, 4790
TspGWI ACGGA 4 cut(s) 421, 1184, 1684, 4879
Van91I CCANNNNNTGG 3 cut(s) 2836, 3593, 4732
VpaK11BI GGWCC 3 cut(s) 223, 3820, 4979
XbaI TCTAGA 2 cut(s) 500, 4316
XcmI CCANNNNNNNNNTGG 1 cut(s) 2600
XhoI CTCGAG 2 cut(s) 1676, 2889
XmiI GTMKAC 2 cut(s) 1666, 4345
XmnI GAANNNNTTC 2 cut(s) 2421, 3060
XspI CTAG 7 cut(s) 42, 447, 501, 816, 990, 1505, 4317
ZrmI AGTACT 1 cut(s) 2459
Zsp2I ATGCAT 2 cut(s) 3655, 3778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.