pycom11g18470

Indole-3-acetate O-methyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008751
Physical Location & Seq
Forward (+)
20618203 .. 20621404
3202 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
ATGACTTCAACACGCTGTTCCAGCTCATCCCTCCTATGGCCAACCACGGCGGCAGCATGGAGGAAACGCTTGCCGCTGACAACCACCGCTCCTACTTTGCTGCTGCCGTCCCAGGTTCATTTTACCGGCGGCTCTTCCCATCGAGGTCCGTTGACCTTTTTCACTCGGCGTTCTCCTTGCATTGGCTCTCTCAGGTTCCAGAGAGTGTGCTGGACAAGAGATCAGCAGCCTACAACAAAGGAAGGGTGTTTATTCATGGGGCAAATGAGAGCACAGCTATTGCCTACAAGAAGCAGTTCCAGACAGACTTGGCAAGCTTTCTCAGGTCCAGAGCCAAGGAGCTCAAGAAGGGAGGCTCCATGTTTCTTGTCTGCTTGGGTAGGACCACAGTGGACCCCACTGACGAAAGTGGCCCAGGCCTTCTCTTTGGGACCCACTTTCAGGATGCTTGGGATGATCGATCTTGTCCAAGAGGGACTTATCACTAGTGAGAAACGTGACAGTTTCAACATTCCTGTGTATGCATCAAGTTTACAAGACTTCAAGGAAGTAGTGGAAGCCGATGGCTCATTCACCATAAACAAGCTCGAGATTTTTAAAGGAGGAAGTCCCCTTGTTGTGAGCGAACCTGATGATGCTGTTGAAGTCGGTCGAGCCCTTGCCAACAGTTGTAGGAGCGTTGCTGGAGTCCTCGTCGATGCACACATCGGTGACCACCTTGGCAATGAGTTGTTCTCAAGGGTGGAGAAAAGGGGCACGAGCCAAGCCAAAGAGCTATTGGAGAAGTTACAATTCTTTCACATAGTGGCCTCACTTTCTCTTGTTTAA

Protein Analysis

276

Amino Acids

30.36

Weight (kDa)

9.46

Isoelectric Point (pI)

54.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 153 - 273 6.6e-25 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 89
AciI CCGC 4 cut(s) 50, 74, 87, 129
AcoI YGGCCR 1 cut(s) 38
AdeI CACNNNGTG 1 cut(s) 805
AfiI CCNNNNNNNGG 3 cut(s) 36, 182, 441
AgsI TTSAA 4 cut(s) 9, 508, 544, 644
AhlI ACTAGT 1 cut(s) 485
AjnI CCWGG 2 cut(s) 111, 414
AleI CACNNNNGTG 1 cut(s) 708
AluBI AGCT 6 cut(s) 24, 277, 317, 342, 586, 775
AluI AGCT 6 cut(s) 24, 277, 317, 342, 586, 775
Alw21I GWGCWC 2 cut(s) 274, 344
Ama87I CYCGRG 1 cut(s) 587
AoxI GGCC 4 cut(s) 38, 411, 417, 807
ApeKI GCWGC 4 cut(s) 53, 100, 103, 226
Asp700I GAANNNNTTC 1 cut(s) 295
AspS9I GGNCC 6 cut(s) 146, 326, 383, 393, 412, 431
AsuHPI GGTGA 2 cut(s) 565, 722
AvaI CYCGRG 1 cut(s) 587
AvaII GGWCC 5 cut(s) 146, 326, 383, 393, 431
BaeGI GKGCMC 1 cut(s) 758
BalI TGGCCA 1 cut(s) 40
BanII GRGCYC 2 cut(s) 344, 658
BauI CACGAG 1 cut(s) 757
Bbv12I GWGCWC 2 cut(s) 274, 344
BbvI GCAGC 4 cut(s) 65, 87, 90, 238
BccI CCATC 2 cut(s) 147, 557
BceAI ACGGC 2 cut(s) 63, 91
BciT130I CCWGG 2 cut(s) 113, 416
BcuI ACTAGT 1 cut(s) 485
BfaI CTAG 1 cut(s) 486
BisI GCNGC 7 cut(s) 51, 54, 74, 101, 104, 130, 227
BlsI GCNGC 7 cut(s) 52, 55, 75, 102, 105, 131, 228
Bme1390I CCNGG 2 cut(s) 113, 416
Bme18I GGWCC 5 cut(s) 146, 326, 383, 393, 431
BmeT110I CYCGRG 1 cut(s) 587
BmgT120I GGNCC 6 cut(s) 146, 326, 383, 393, 412, 431
BmiI GGNNCC 5 cut(s) 197, 357, 395, 432, 433
BmrFI CCNGG 2 cut(s) 113, 416
BmsI GCATC 4 cut(s) 435, 533, 625, 688
BplI GAGNNNNNCTC 2 cut(s) 719, 751
BpmI CTGGAG 1 cut(s) 705
BpuEI CTTGAG 2 cut(s) 328, 721
Bsa29I ATCGAT 1 cut(s) 459
BsaJI CCNNGG 5 cut(s) 45, 111, 335, 414, 718
BsaXI ACNNNNNCTCC 4 cut(s) 73, 103, 678, 708
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 182, 441
Bse118I RCCGGY 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 730
BseBI CCWGG 2 cut(s) 113, 416
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 5 cut(s) 45, 111, 335, 414, 718
BseGI GGATG 3 cut(s) 26, 450, 459
BseLI CCNNNNNNNGG 3 cut(s) 36, 182, 441
BseMI GCAATG 1 cut(s) 730
BseMII CTCAG 2 cut(s) 205, 336
BseSI GKGCMC 1 cut(s) 758
BseXI GCAGC 4 cut(s) 65, 87, 90, 238
Bsh1285I CGRYCG 1 cut(s) 652
BshFI GGCC 4 cut(s) 40, 413, 419, 809
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 652
BsiHKAI GWGCWC 2 cut(s) 274, 344
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 1 cut(s) 126
BslFI GGGAC 4 cut(s) 94, 444, 489, 594
BslI CCNNNNNNNGG 3 cut(s) 36, 182, 441
BsmFI GGGAC 4 cut(s) 94, 444, 489, 594
BsnI GGCC 4 cut(s) 40, 413, 419, 809
BsoBI CYCGRG 1 cut(s) 587
Bsp1286I GDGCHC 4 cut(s) 274, 344, 658, 758
Bsp143I GATC 3 cut(s) 220, 456, 460
BspACI CCGC 4 cut(s) 50, 74, 87, 129
BspANI GGCC 4 cut(s) 40, 413, 419, 809
BspCNI CTCAG 2 cut(s) 204, 335
BspDI ATCGAT 1 cut(s) 459
BspLI GGNNCC 5 cut(s) 197, 357, 395, 432, 433
BspQI GCTCTTC 1 cut(s) 139
BsrBI CCGCTC 1 cut(s) 89
BsrDI GCAATG 1 cut(s) 730
BsrFI RCCGGY 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 5 cut(s) 45, 111, 335, 414, 718
BssMI GATC 3 cut(s) 220, 456, 460
BssSI CACGAG 1 cut(s) 757
BssT1I CCWWGG 2 cut(s) 335, 718
Bst2BI CACGAG 1 cut(s) 757
Bst2UI CCWGG 2 cut(s) 113, 416
Bst4CI ACNGT 3 cut(s) 390, 503, 668
Bst6I CTCTTC 1 cut(s) 139
BstC8I GCNNGC 2 cut(s) 71, 315
BstDEI CTNAG 2 cut(s) 191, 322
BstDSI CCRYGG 1 cut(s) 45
BstEII GGTNACC 1 cut(s) 710
BstF5I GGATG 3 cut(s) 26, 450, 459
BstKTI GATC 3 cut(s) 223, 459, 463
BstMBI GATC 3 cut(s) 220, 456, 460
BstMCI CGRYCG 1 cut(s) 652
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstNI CCWGG 2 cut(s) 113, 416
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 111, 414
BstSLI GKGCMC 1 cut(s) 758
BstV1I GCAGC 4 cut(s) 65, 87, 90, 238
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 4 cut(s) 40, 413, 419, 809
BsuTUI ATCGAT 1 cut(s) 459
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 3 cut(s) 26, 450, 459
BtsIMutI CAGTG 2 cut(s) 395, 398
Cac8I GCNNGC 2 cut(s) 71, 315
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 6 cut(s) 146, 326, 383, 393, 412, 431
ClaI ATCGAT 1 cut(s) 459
CviAII CATG 3 cut(s) 57, 256, 360
DdeI CTNAG 2 cut(s) 191, 322
DpnI GATC 3 cut(s) 222, 458, 462
DpnII GATC 3 cut(s) 220, 456, 460
DraI TTTAAA 1 cut(s) 598
DraIII CACNNNGTG 1 cut(s) 805
EaeI YGGCCR 1 cut(s) 38
Eam1104I CTCTTC 1 cut(s) 139
EarI CTCTTC 1 cut(s) 139
Ecl136II GAGCTC 1 cut(s) 342
Eco130I CCWWGG 2 cut(s) 335, 718
Eco147I AGGCCT 1 cut(s) 419
Eco24I GRGCYC 2 cut(s) 344, 658
Eco47I GGWCC 5 cut(s) 146, 326, 383, 393, 431
Eco53kI GAGCTC 1 cut(s) 342
Eco88I CYCGRG 1 cut(s) 587
Eco91I GGTNACC 1 cut(s) 710
EcoICRI GAGCTC 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 431
EcoO65I GGTNACC 1 cut(s) 710
EcoRII CCWGG 2 cut(s) 111, 414
EcoT14I CCWWGG 2 cut(s) 335, 718
EcoT22I ATGCAT 1 cut(s) 526
EcoT38I GRGCYC 2 cut(s) 344, 658
ErhI CCWWGG 2 cut(s) 335, 718
FaeI CATG 3 cut(s) 60, 259, 363
FaiI YATR 7 cut(s) 37, 58, 257, 361, 522, 578, 803
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 4 cut(s) 94, 444, 489, 594
FatI CATG 3 cut(s) 56, 255, 359
Fnu4HI GCNGC 7 cut(s) 51, 54, 74, 101, 104, 130, 227
FokI GGATG 3 cut(s) 13, 457, 466
FriOI GRGCYC 2 cut(s) 344, 658
Fsp4HI GCNGC 7 cut(s) 51, 54, 74, 101, 104, 130, 227
FspBI CTAG 1 cut(s) 486
GluI GCNGC 7 cut(s) 51, 54, 74, 101, 104, 130, 227
GsuI CTGGAG 1 cut(s) 705
HaeIII GGCC 4 cut(s) 40, 413, 419, 809
HapII CCGG 1 cut(s) 126
Hin1II CATG 3 cut(s) 60, 259, 363
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 1 cut(s) 687
HpaII CCGG 1 cut(s) 126
HphI GGTGA 2 cut(s) 565, 722
Hpy166II GTNNAC 3 cut(s) 153, 393, 533
Hpy188III TCNNGA 6 cut(s) 199, 300, 329, 345, 442, 589
Hpy8I GTNNAC 3 cut(s) 153, 393, 533
Hpy99I CGWCG 1 cut(s) 698
HpyAV CCTTC 3 cut(s) 236, 342, 430
HpyCH4III ACNGT 3 cut(s) 390, 503, 668
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 3 cut(s) 180, 524, 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpyF3I CTNAG 2 cut(s) 191, 322
HpySE526I ACGT 1 cut(s) 496
Hsp92II CATG 3 cut(s) 60, 259, 363
KflI GGGWCCC 1 cut(s) 431
Kzo9I GATC 3 cut(s) 220, 456, 460
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 4 cut(s) 94, 339, 361, 675
Lsp1109I GCAGC 4 cut(s) 65, 87, 90, 238
LweI GCATC 4 cut(s) 435, 533, 625, 688
MaeI CTAG 1 cut(s) 486
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 3 cut(s) 497, 710, 786
MalI GATC 3 cut(s) 222, 458, 462
MbiI CCGCTC 1 cut(s) 89
MboI GATC 3 cut(s) 220, 456, 460
MboII GAAGA 1 cut(s) 126
MhlI GDGCHC 4 cut(s) 274, 344, 658, 758
MlsI TGGCCA 1 cut(s) 40
MluCI AATT 1 cut(s) 791
MluNI TGGCCA 1 cut(s) 40
MlyI GAGTC 1 cut(s) 696
MnlI CCTC 8 cut(s) 41, 54, 137, 346, 466, 596, 701, 820
Mox20I TGGCCA 1 cut(s) 40
Mph1103I ATGCAT 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 295
MscI TGGCCA 1 cut(s) 40
MseI TTAA 2 cut(s) 597, 826
MslI CAYNNNNRTG 2 cut(s) 515, 708
Msp20I TGGCCA 1 cut(s) 40
MspA1I CMGCKG 1 cut(s) 76
MspI CCGG 1 cut(s) 126
MspR9I CCNGG 2 cut(s) 113, 416
MvaI CCWGG 2 cut(s) 113, 416
MwoI GCNNNNNNNGC 1 cut(s) 21
NdeII GATC 3 cut(s) 220, 456, 460
NlaIII CATG 3 cut(s) 60, 259, 363
NlaIV GGNNCC 5 cut(s) 197, 357, 395, 432, 433
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 2 cut(s) 497, 710
NsiI ATGCAT 1 cut(s) 526
OliI CACNNNNGTG 1 cut(s) 708
PaeR7I CTCGAG 1 cut(s) 587
PceI AGGCCT 1 cut(s) 419
PciSI GCTCTTC 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 295
PkrI GCNGC 7 cut(s) 52, 55, 75, 102, 105, 131, 228
PleI GAGTC 1 cut(s) 695
PpsI GAGTC 1 cut(s) 695
PpuMI RGGWCCY 1 cut(s) 431
Psp124BI GAGCTC 1 cut(s) 344
Psp5II RGGWCCY 1 cut(s) 431
Psp6I CCWGG 2 cut(s) 111, 414
PspEI GGTNACC 1 cut(s) 710
PspGI CCWGG 2 cut(s) 111, 414
PspN4I GGNNCC 5 cut(s) 197, 357, 395, 432, 433
PspPI GGNCC 6 cut(s) 146, 326, 383, 393, 412, 431
PspPPI RGGWCCY 1 cut(s) 431
RseI CAYNNNNRTG 2 cut(s) 515, 708
SacI GAGCTC 1 cut(s) 344
SapI GCTCTTC 1 cut(s) 139
SaqAI TTAA 2 cut(s) 597, 826
SatI GCNGC 7 cut(s) 51, 54, 74, 101, 104, 130, 227
Sau3AI GATC 3 cut(s) 220, 456, 460
Sau96I GGNCC 6 cut(s) 146, 326, 383, 393, 412, 431
SchI GAGTC 1 cut(s) 696
ScrFI CCNGG 2 cut(s) 113, 416
SduI GDGCHC 4 cut(s) 274, 344, 658, 758
SfaNI GCATC 4 cut(s) 435, 533, 625, 688
Sfr274I CTCGAG 1 cut(s) 587
SinI GGWCC 5 cut(s) 146, 326, 383, 393, 431
SlaI CTCGAG 1 cut(s) 587
SmiMI CAYNNNNRTG 2 cut(s) 515, 708
SmlI CTYRAG 3 cut(s) 343, 587, 736
SmoI CTYRAG 3 cut(s) 343, 587, 736
SpeI ACTAGT 1 cut(s) 485
Sse9I AATT 1 cut(s) 791
SseBI AGGCCT 1 cut(s) 419
SsiI CCGC 4 cut(s) 50, 74, 87, 129
SspMI CTAG 1 cut(s) 486
SstI GAGCTC 1 cut(s) 344
StuI AGGCCT 1 cut(s) 419
StyD4I CCNGG 2 cut(s) 111, 414
StyI CCWWGG 2 cut(s) 335, 718
TaaI ACNGT 3 cut(s) 390, 503, 668
TaiI ACGT 1 cut(s) 499
TaqI TCGA 5 cut(s) 142, 459, 588, 652, 696
TaqII GACCGA 1 cut(s) 638
TasI AATT 1 cut(s) 791
TauI GCSGC 3 cut(s) 53, 76, 132
Tru1I TTAA 2 cut(s) 597, 826
Tru9I TTAA 2 cut(s) 597, 826
TscAI CASTG 2 cut(s) 395, 405
TseFI GTSAC 2 cut(s) 497, 710
TseI GCWGC 4 cut(s) 53, 100, 103, 226
Tsp45I GTSAC 2 cut(s) 497, 710
TspDTI ATGAA 2 cut(s) 107, 244
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 2 cut(s) 395, 405
VpaK11BI GGWCC 5 cut(s) 146, 326, 383, 393, 431
XcmI CCANNNNNNNNNTGG 1 cut(s) 775
XhoI CTCGAG 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 295
XspI CTAG 1 cut(s) 486
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.