pycom11g20080

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009516
Physical Location & Seq
Reverse (-)
22614056 .. 22616064
2009 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 627 bp
ATGATTACTACTACTACTACTACTACTAGGAAGCAGCTCGAAGTCGTAAGAGACGATCAGACTCCGCAGAAATGGTGTGTTTTATTGAGAGAAGATGTGTTCAGAAGATTCATGGCTCAAGGCAGCACAAATCCAACGGTGCAGAAGGTCTTTGGTGGTGCATCTTTTTTCTGTCCTTTGCTGTTTGGGAAATTTTTTGATCCTTCTGATGCCTTCCCGCTGTGGGAGTTTGAGTCGGATATCTTGCTGTCTGGTCTCAGAAGCTCTGGCCAAACCTGTATTGATTGGTTTCAAACAGATCAAGACTATGTCCTAAAAGTAGAACTACCAGGAGAAGGGAAAAACAATGTTCAAGTGTATGCTGAAAATGGAAAAGTGGTGGAAATAGGTGGGCAGTGGAAGCAGCAGCAAGGAGGAGAGTCCAACTCCTTGAAGAGCACAAAGGATTGGAGAAGTGGGAATTGGTGGGAACATGGTTATGTTCGGAGGCTTGAGCTCCCACAAGATGCTGATTCCAGAAGGATAGAGGCTTCTCTCACTAATGACCTACTTTTGGAACTCAAAGTTCACAAGATCAACAAACCCTTGGATTCTAATGACAATGCAAAAGATAAAGATCCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

209

Amino Acids

23.81

Weight (kDa)

5.33

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 95 - 195 1.7e-09 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 65, 218
AclWI GGATC 2 cut(s) 194, 611
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 1 cut(s) 191
AfiI CCNNNNNNNGG 3 cut(s) 223, 335, 553
AgsI TTSAA 3 cut(s) 293, 353, 433
AjnI CCWGG 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 445, 477
AluBI AGCT 3 cut(s) 37, 264, 496
AluI AGCT 3 cut(s) 37, 264, 496
Alw21I GWGCWC 2 cut(s) 440, 498
Alw26I GTCTC 2 cut(s) 45, 260
AlwI GGATC 2 cut(s) 194, 611
AoxI GGCC 1 cut(s) 268
ApeKI GCWGC 4 cut(s) 34, 123, 403, 406
ApoI RAATTY 1 cut(s) 191
BalI TGGCCA 1 cut(s) 270
BanII GRGCYC 1 cut(s) 498
Bbv12I GWGCWC 2 cut(s) 440, 498
BbvI GCAGC 4 cut(s) 46, 135, 415, 418
BciT130I CCWGG 1 cut(s) 330
BcoDI GTCTC 2 cut(s) 45, 260
BfaI CTAG 1 cut(s) 27
BisI GCNGC 4 cut(s) 35, 124, 404, 407
BlsI GCNGC 4 cut(s) 36, 125, 405, 408
Bme1390I CCNGG 1 cut(s) 330
BmrFI CCNGG 1 cut(s) 330
BmsI GCATC 3 cut(s) 170, 199, 496
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BpuEI CTTGAG 2 cut(s) 102, 512
BsaBI GATNNNNATC 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 1 cut(s) 585
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 335, 553
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 585
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 3 cut(s) 223, 335, 553
BseMII CTCAG 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 429
BseXI GCAGC 4 cut(s) 46, 135, 415, 418
BsgI GTGCAG 1 cut(s) 161
BshFI GGCC 1 cut(s) 270
BsiHKAI GWGCWC 2 cut(s) 440, 498
BslI CCNNNNNNNGG 3 cut(s) 223, 335, 553
BsmAI GTCTC 2 cut(s) 45, 260
BsmBI CGTCTC 1 cut(s) 45
BsnI GGCC 1 cut(s) 270
Bso31I GGTCTC 1 cut(s) 260
Bsp1286I GDGCHC 2 cut(s) 440, 498
Bsp143I GATC 5 cut(s) 55, 199, 298, 573, 616
BspACI CCGC 2 cut(s) 65, 218
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 1 cut(s) 270
BspPI GGATC 2 cut(s) 194, 611
BspQI GCTCTTC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 5 cut(s) 55, 199, 298, 573, 616
BssT1I CCWWGG 1 cut(s) 585
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 428
BstDEI CTNAG 1 cut(s) 257
BstKTI GATC 5 cut(s) 58, 202, 301, 576, 619
BstMAI GTCTC 2 cut(s) 45, 260
BstMBI GATC 5 cut(s) 55, 199, 298, 573, 616
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 1 cut(s) 328
BstV1I GCAGC 4 cut(s) 46, 135, 415, 418
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
BsuRI GGCC 1 cut(s) 270
BtsI GCAGTG 1 cut(s) 401
BtsIMutI CAGTG 1 cut(s) 401
CviAII CATG 2 cut(s) 112, 473
CviJI RGCY 7 cut(s) 37, 116, 264, 270, 490, 496, 530
CviKI_1 RGCY 7 cut(s) 37, 116, 264, 270, 490, 496, 530
DdeI CTNAG 1 cut(s) 257
DpnI GATC 5 cut(s) 57, 201, 300, 575, 618
DpnII GATC 5 cut(s) 55, 199, 298, 573, 616
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 428
EarI CTCTTC 1 cut(s) 428
Ecl136II GAGCTC 1 cut(s) 496
Eco130I CCWWGG 1 cut(s) 585
Eco24I GRGCYC 1 cut(s) 498
Eco31I GGTCTC 1 cut(s) 260
Eco32I GATATC 1 cut(s) 241
Eco53kI GAGCTC 1 cut(s) 496
EcoICRI GAGCTC 1 cut(s) 496
EcoRII CCWGG 1 cut(s) 328
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 1 cut(s) 585
EcoT38I GRGCYC 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 585
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 2 cut(s) 115, 476
FaiI YATR 5 cut(s) 113, 309, 360, 474, 480
FatI CATG 2 cut(s) 111, 472
FauI CCCGC 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 35, 124, 404, 407
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 4 cut(s) 35, 124, 404, 407
FspBI CTAG 1 cut(s) 27
GluI GCNGC 4 cut(s) 35, 124, 404, 407
HaeIII GGCC 1 cut(s) 270
Hin1II CATG 2 cut(s) 115, 476
HinfI GANTC 6 cut(s) 61, 108, 233, 419, 512, 590
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 6 cut(s) 60, 104, 208, 238, 260, 486
Hpy188III TCNNGA 2 cut(s) 302, 516
Hpy8I GTNNAC 1 cut(s) 568
HpyAV CCTTC 5 cut(s) 139, 213, 223, 329, 513
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 3 cut(s) 142, 161, 605
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 1 cut(s) 257
Hsp92II CATG 2 cut(s) 115, 476
Kzo9I GATC 5 cut(s) 55, 199, 298, 573, 616
LguI GCTCTTC 1 cut(s) 428
LmnI GCTCC 1 cut(s) 501
LpnPI CCDG 6 cut(s) 237, 252, 289, 315, 342, 529
Lsp1109I GCAGC 4 cut(s) 46, 135, 415, 418
LweI GCATC 3 cut(s) 170, 199, 496
MaeI CTAG 1 cut(s) 27
MalI GATC 5 cut(s) 57, 201, 300, 575, 618
MboI GATC 5 cut(s) 55, 199, 298, 573, 616
MboII GAAGA 3 cut(s) 104, 117, 445
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 2 cut(s) 440, 498
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 2 cut(s) 191, 460
MluNI TGGCCA 1 cut(s) 270
MlyI GAGTC 3 cut(s) 55, 242, 428
MmeI TCCRAC 3 cut(s) 158, 216, 447
MnlI CCTC 3 cut(s) 407, 480, 520
Mox20I TGGCCA 1 cut(s) 270
MscI TGGCCA 1 cut(s) 270
MslI CAYNNNNRTG 1 cut(s) 477
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 220
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
MwoI GCNNNNNNNGC 1 cut(s) 400
NdeII GATC 5 cut(s) 55, 199, 298, 573, 616
NlaIII CATG 2 cut(s) 115, 476
PciSI GCTCTTC 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfeI GAWTC 3 cut(s) 108, 512, 590
PflFI GACNNNGTC 1 cut(s) 308
PkrI GCNGC 4 cut(s) 36, 125, 405, 408
PleI GAGTC 3 cut(s) 55, 241, 427
PpsI GAGTC 3 cut(s) 55, 241, 427
Psp124BI GAGCTC 1 cut(s) 498
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PsuI RGATCY 1 cut(s) 616
PsyI GACNNNGTC 1 cut(s) 308
RseI CAYNNNNRTG 1 cut(s) 477
SacI GAGCTC 1 cut(s) 498
SapI GCTCTTC 1 cut(s) 428
SatI GCNGC 4 cut(s) 35, 124, 404, 407
Sau3AI GATC 5 cut(s) 55, 199, 298, 573, 616
SchI GAGTC 3 cut(s) 55, 242, 428
ScrFI CCNGG 1 cut(s) 330
SduI GDGCHC 2 cut(s) 440, 498
SetI ASST 7 cut(s) 39, 150, 266, 278, 391, 498, 549
SfaNI GCATC 3 cut(s) 170, 199, 496
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 2 cut(s) 117, 491
SmoI CTYRAG 2 cut(s) 117, 491
Sse9I AATT 2 cut(s) 191, 460
SsiI CCGC 2 cut(s) 65, 218
SspMI CTAG 1 cut(s) 27
SstI GAGCTC 1 cut(s) 498
StyD4I CCNGG 1 cut(s) 328
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 39
TasI AATT 2 cut(s) 191, 460
TfiI GAWTC 3 cut(s) 108, 512, 590
TscAI CASTG 1 cut(s) 401
TseI GCWGC 4 cut(s) 34, 123, 403, 406
TspDTI ATGAA 1 cut(s) 100
TspRI CASTG 1 cut(s) 401
Tth111I GACNNNGTC 1 cut(s) 308
XapI RAATTY 1 cut(s) 191
XspI CTAG 1 cut(s) 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.