pycom11g21830

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008363
Physical Location & Seq
Forward (+)
24845956 .. 24853590
7635 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3174 bp
ATGAGGGGTCCTATGGGGGCAGTCATAGGGAGGTACCCCCCAAGTGATGGTAGTGCTCAAATGGGTTCAATCATTAGGCACAACAGGAAATGCAGGGATCTTGTCTTCCTCGTAATCTTCATAGCCTTCTGGGTTGCCATGATTGTTAACTCCAGCTTTGGATTCAACCAAGGAAACCCTTCAAGGCTCACTTATGGACTGGACTACAAAGGAAATGTTTGTGGTGACAAGCATGCAAAACCTGGTCTTAAGGAGCTGGAACTCAAATATTGGTTAAATCCTAACCAGGTTTACCTTAGTGGTTTAAAAGACAATCAATTCAAGTTGGCCAATGCTCGGAGTATATGCTTATTGGATTGCCCTATCCCATATGAAGATTCACTAAGTTGGGTGTGTGATTATCCTGATGGAGATATCCGTCTCTCAATGGATGATTGGATCGACAGGAATTATGATTATTATGAGTTCCTTACACCAGAATTGAGAAACACCTCTCTTCAACTTCAGGGTCCTTGTTACCCTGTCATATTTCCCAGTATAAATGTTTATTGGACTTGCCAATTTATTGCTCGTCCCTCCAACGTATCTTTGACGCATTGGCAGCAAATGGGTGGAGTGAAAATCAATGAGGACATTATTATAGACAAATCTATTCACAAGGCCATCAATTCTCGGTCATCTGTCTTAAAGAGATACATGGCTGATATTGGAAAAGCATGGCCGGTGCTGATTGTTTGTGGAGGAATTTTGCCACTTTTTCTGTCAGTGATTTGGCTGCTTCTGATTCGTCATTTTGTTGCTGCAATGCCGTGGATTACAGTCGGTCTCTTTGACATTCTGATGATAGCAGTCACAATGTTCTTTTACTTAAAAGCCGGATGGATAGGAAATGATAGCATCTCCCCAATCATTGGTGAGCACGATCCATACATTCGCATAATTGGAAGGGAATTACATCATCTCCGTGCTGTTGCTGTTATTATGACCTCTATTATGGTTGTTTCTGTCCTTACATCAATAGCTATAGTCCGCCGCATCCTTATGGCAACGTCTGTCCTCAAGGTTGCTGCAAAGGTCATAGGAGAAGCTCAGGCGCTCATAATATTCCCAGTCATTCCATATACCATCCTTGCAATTTTCTACATGTTCTGGTTTTCAGCTGCTTTCCATCTGTTCAGTTCTGGTCAGGTTGTCCAGAATGACTGCAATTTGAACTGCTGTGCTTATGATCTATTGTCAAAACGAGTGAACTGTGATCGTTGTTGCGGTTATAGCATTCATTACACTCCTCATATTGGAGTTGCCATCCTTTTCCACCTATTTGGGGGTTATTGGGCTACCCAGTTTTTCATAGCATGCTCTTCCACAGTTATTGCGGGTGCTGTTGCTTCTTATTATTGGGCTCGTGGTGAAACTTCACCAGAAATTCCATTTCTTCCAGTCTTTGCCTCCATGAAACGCCTTGCACGCTATAACCTTGGGTCGGTTGCTCTCGGCTCCCTGATAGTGTCATTTGTGGAATCAATCCGCTTTATGCTTGAGTCAATTCGTCGCAGGCTAAAAGTGGCTGGAACCACACCCGATAACTGGTTTGGAAAGGCCGTGTACCATACTTCTAGATTTTTCCTGAGGTGTATTGAGTGGACAATCAAATCAGTCAATCGCAATGCCTACATTATGATTGCTATAACGGGTAAAAGCTTCTGCAAGGCTTCTGCAATCGCAACAGATTTGATCATTAGTAACATTCTTCGGATAGGGAAGGTGAACGTGATTGGAGACATCATCCTATTCCTTGGAAAATTATGTGTTAGCCTCTCAACAGCCCTTTTCGCTTTCCTTATGTTGGATACCCACAAATACAAATCTGGCCACAACAAGATCACTTCTCCACTGTTTCCTGTGTTGGTTTGCTGGGCTCTCGGTTATGTGGTTGCCACTCTTTTCTTTGCTGTGGTGGAGATGTCAATCGATACCATCTTTCTTTCATTCTGCCAGGATTCTGACGAGCACCAAGGGACTGCCCAGTATGCTCCGCCACTTCTGATCGAAACTCTAAATGACCAAAACGAGATGCAGAGACTCACCCAAGGACCCCAGTCAAATAGCTCAGATTCCTTGAATTTTTGTGTTCTACCTGTATATATTCAAGTGTTAGGCGAAACCGAGAGGGAAAACGGGTGCCTTTATACAAGTTTAGAATATTTGTTGTTGCATTTGAGATATGTCACATCCCAACCCGGGGCGGATCACTTTCCGGGTCCGCTCCACCACCGTAGCACGATATTGTCCGCTTTGGGCTTACCATTCCCGATATTGTCCGCTTTGGGCTTACCATTCCCTTACGGTTTTGTTTTTGGGAACTCACGAGCAACTTCCCAGTGGGTCACCCATTATGGGATTGCTCTCGCGCGCTACTCGCTTAACTTCGGAGTTCCCATGGAACCCGAAGCCAGTGAGCTCCCAAAAGGCCTCATGCTAGGTAAGGATGAGAATATTTATTTAAGGATCACTTCTTTAGGCGATGTGGGATGTCACAAGATACATTTGAAAGAAATTATTGAAATTAGAGCAATTATGCAATACTACAAATCTCTTGTGAAATTATTGAAATTGTCTTGCATCCTTGCGAAGTTTTTCTTCCACCATTGCGAAGCTTTGTCCGATGCAAATCCGAGGACCTCGTCCGAGAGGGAAGTACATAAATTGTTCTTTGTTGGACCTCATATTCAGACAACGATCTCAACTTTTGAGGAAGAATTAAACGACCGCCAAACTTCTGCTTCAAGTAATGATGCCGGAGTACAAATCTGCTGTGGGACTTGCATAAATGTAAGAATTTCTGGTGAGAAAAGCGGTGACTTCGAGCCCTGTAAAGATCGTATGAAACTGAGCGAGCCTTTTGGTTGGAATTGCAGAGCATGTCGAGCAGAATTTGAGACAGCAAAAAGCCATCCGAAACCAAGCCAGCATATGATTTCAAGCGGCACAGTGCCAAAATTCCTCCAGTCCATCAGTAGCGAAGCAGCTAAAGCCCCTACAGTATATACTAGTAAAGCCACAACGAACGCCTTCTTCTCCATGACCCGGGAACAATTGAATGGCAATGAACAGAATATGAGATGTGATCATAAGGTGGAGGAGGCGATGAAACAGGATAAGAGAAGCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000139 GO:0000325 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0005215 GO:0005326 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006836 GO:0007275 GO:0007389 GO:0008104 GO:0008150 GO:0008324 GO:0008519 GO:0009505 GO:0009506 GO:0009524 GO:0009551 GO:0009663 GO:0009705 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010008 GO:0010051 GO:0010065 GO:0010067 GO:0010087 GO:0010088 GO:0010154 GO:0010496 GO:0010497 GO:0010817 GO:0012505 GO:0015031 GO:0015075 GO:0015101 GO:0015220 GO:0015695 GO:0015696 GO:0015833 GO:0015871 GO:0016020 GO:0016043 GO:0016192 GO:0022414 GO:0022603 GO:0022604 GO:0022622 GO:0022857 GO:0030054 GO:0030100 GO:0030154 GO:0030312 GO:0031090 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0033036 GO:0034220 GO:0034330 GO:0040008 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044426 GO:0044431 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044462 GO:0044464 GO:0045184 GO:0045216 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051510 GO:0055044 GO:0055085 GO:0055088 GO:0060627 GO:0061458 GO:0065007 GO:0065008 GO:0071702 GO:0071705 GO:0071840 GO:0071944 GO:0072488 GO:0090603 GO:0097218 GO:0097708 GO:0098588 GO:0098655 GO:0098791 GO:0098805 GO:0099402 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko05231 map05231
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

1058

Amino Acids

118.65

Weight (kDa)

8.53

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 326 - 668 1.8e-89 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 33
AccB1I GGYRCC 2 cut(s) 33, 2181
AccB7I CCANNNNNTGG 2 cut(s) 47, 911
AccBSI CCGCTC 1 cut(s) 2266
AccII CGCG 2 cut(s) 2411, 2413
AclWI GGATC 5 cut(s) 105, 446, 917, 2256, 2516
AcoI YGGCCR 3 cut(s) 327, 719, 1870
AcsI RAATTY 6 cut(s) 744, 1425, 2122, 2838, 2933, 2999
AcuI CTGAAG 1 cut(s) 488
AfaI GTAC 4 cut(s) 35, 1607, 2700, 2805
AflII CTTAAG 1 cut(s) 248
AflIII ACRYGT 1 cut(s) 1143
AhlI ACTAGT 1 cut(s) 3050
AjnI CCWGG 3 cut(s) 241, 285, 1995
Alw21I GWGCWC 4 cut(s) 58, 921, 2013, 2463
Alw26I GTCTC 5 cut(s) 425, 830, 1773, 2074, 2933
AlwI GGATC 5 cut(s) 105, 446, 917, 2256, 2516
Ama87I CYCGRG 2 cut(s) 2240, 3087
AoxI GGCC 6 cut(s) 327, 660, 719, 1599, 1870, 2470
ApeKI GCWGC 6 cut(s) 601, 775, 800, 1067, 1160, 3026
ApoI RAATTY 6 cut(s) 744, 1425, 2122, 2838, 2933, 2999
Asp700I GAANNNNTTC 3 cut(s) 178, 2636, 3071
Asp718I GGTACC 1 cut(s) 33
AspLEI GCGC 3 cut(s) 1096, 2413, 2415
AspS9I GGNCC 6 cut(s) 8, 509, 2093, 2261, 2679, 2720
AsuC2I CCSGG 5 cut(s) 2241, 2242, 2259, 3088, 3089
AsuHPI GGTGA 9 cut(s) 236, 926, 1410, 1421, 1777, 2077, 2380, 2858, 2870
AvaI CYCGRG 2 cut(s) 2240, 3087
AvaII GGWCC 6 cut(s) 8, 509, 2093, 2261, 2679, 2720
AxyI CCTNAGG 1 cut(s) 1628
BalI TGGCCA 2 cut(s) 329, 1872
BanI GGYRCC 2 cut(s) 33, 2181
BanII GRGCYC 4 cut(s) 1405, 1921, 2463, 2871
BauI CACGAG 2 cut(s) 1404, 2367
BbsI GAAGAC 1 cut(s) 97
Bbv12I GWGCWC 4 cut(s) 58, 921, 2013, 2463
BbvI GCAGC 6 cut(s) 613, 762, 787, 1054, 1147, 3038
BceAI ACGGC 2 cut(s) 793, 1586
BciT130I CCWGG 3 cut(s) 243, 287, 1997
BciVI GTATCC 1 cut(s) 1843
BclI TGATCA 2 cut(s) 1734, 3127
BcnI CCSGG 5 cut(s) 2241, 2242, 2259, 3088, 3089
BcoDI GTCTC 5 cut(s) 425, 830, 1773, 2074, 2933
BcuI ACTAGT 1 cut(s) 3050
BfaI CTAG 3 cut(s) 1617, 2480, 3051
BfmI CTRYAG 2 cut(s) 1023, 3039
BfoI RGCGCY 1 cut(s) 1097
BfrI CTTAAG 1 cut(s) 248
BfuI GTATCC 1 cut(s) 1843
BisI GCNGC 8 cut(s) 602, 776, 801, 1033, 1068, 1161, 2986, 3027
BlsI GCNGC 8 cut(s) 603, 777, 802, 1034, 1069, 1162, 2987, 3028
Bme1390I CCNGG 8 cut(s) 243, 287, 1997, 2241, 2242, 2259, 3088, 3089
Bme18I GGWCC 6 cut(s) 8, 509, 2093, 2261, 2679, 2720
BmeT110I CYCGRG 2 cut(s) 2240, 3087
BmgT120I GGNCC 6 cut(s) 8, 509, 2093, 2261, 2679, 2720
BmiI GGNNCC 9 cut(s) 9, 35, 510, 1498, 1573, 2095, 2183, 2262, 2445
BmrFI CCNGG 8 cut(s) 243, 287, 1997, 2241, 2242, 2259, 3088, 3089
BmrI ACTGGG 6 cut(s) 528, 1103, 1336, 2020, 2092, 2374
BmsI GCATC 6 cut(s) 906, 1044, 2064, 2631, 2656, 2785
BmuI ACTGGG 6 cut(s) 528, 1103, 1336, 2020, 2092, 2374
BoxI GACNNNNGTC 1 cut(s) 2098
BpiI GAAGAC 1 cut(s) 97
BpmI CTGGAG 2 cut(s) 136, 2990
Bpu10I CCTNAGC 1 cut(s) 1089
BpuEI CTTGAG 2 cut(s) 1043, 1559
BpuMI CCSGG 5 cut(s) 2241, 2242, 2259, 3088, 3089
Bsa29I ATCGAT 1 cut(s) 1971
BsaBI GATNNNNATC 2 cut(s) 1967, 2670
BsaI GGTCTC 1 cut(s) 830
BsaXI ACNNNNNCTCC 2 cut(s) 945, 975
Bse118I RCCGGY 1 cut(s) 721
Bse21I CCTNAGG 1 cut(s) 1628
Bse3DI GCAATG 4 cut(s) 810, 1672, 2647, 3112
Bse8I GATNNNNATC 2 cut(s) 1967, 2670
BseBI CCWGG 3 cut(s) 243, 287, 1997
BseCI ATCGAT 1 cut(s) 1971
BseJI GATNNNNATC 2 cut(s) 1967, 2670
BseMI GCAATG 4 cut(s) 810, 1672, 2647, 3112
BseMII CTCAG 4 cut(s) 1103, 1619, 2124, 2882
BsePI GCGCGC 1 cut(s) 2411
BseRI GAGGAG 2 cut(s) 1278, 3155
BseXI GCAGC 6 cut(s) 613, 762, 787, 1054, 1147, 3038
BseYI CCCAGC 1 cut(s) 1914
Bsh1236I CGCG 2 cut(s) 2411, 2413
Bsh1285I CGRYCG 1 cut(s) 2770
BshFI GGCC 6 cut(s) 329, 662, 721, 1601, 1872, 2472
BshNI GGYRCC 2 cut(s) 33, 2181
BshVI ATCGAT 1 cut(s) 1971
BsiEI CGRYCG 1 cut(s) 2770
BsiHKAI GWGCWC 4 cut(s) 58, 921, 2013, 2463
BsiHKCI CYCGRG 2 cut(s) 2240, 3087
BsiSI CCGG 6 cut(s) 722, 876, 2241, 2258, 2799, 3088
BslFI GGGAC 3 cut(s) 558, 2032, 2833
BsmAI GTCTC 5 cut(s) 425, 830, 1773, 2074, 2933
BsmBI CGTCTC 1 cut(s) 425
BsmFI GGGAC 3 cut(s) 558, 2032, 2833
BsmI GAATGC 1 cut(s) 1275
BsnI GGCC 6 cut(s) 329, 662, 721, 1601, 1872, 2472
Bso31I GGTCTC 1 cut(s) 830
BsoBI CYCGRG 2 cut(s) 2240, 3087
Bsp1286I GDGCHC 7 cut(s) 58, 921, 1405, 1921, 2013, 2463, 2871
Bsp19I CCATGG 1 cut(s) 2439
BspANI GGCC 6 cut(s) 329, 662, 721, 1601, 1872, 2472
BspCNI CTCAG 4 cut(s) 1102, 1620, 2123, 2883
BspDI ATCGAT 1 cut(s) 1971
BspFNI CGCG 2 cut(s) 2411, 2413
BspLI GGNNCC 9 cut(s) 9, 35, 510, 1498, 1573, 2095, 2183, 2262, 2445
BspPI GGATC 5 cut(s) 105, 446, 917, 2256, 2516
BspQI GCTCTTC 1 cut(s) 1366
BspT107I GGYRCC 2 cut(s) 33, 2181
BspTI CTTAAG 1 cut(s) 248
BspTNI GGTCTC 1 cut(s) 830
BsrBI CCGCTC 1 cut(s) 2266
BsrDI GCAATG 4 cut(s) 810, 1672, 2647, 3112
BsrFI RCCGGY 1 cut(s) 721
BssAI RCCGGY 1 cut(s) 721
BssHII GCGCGC 1 cut(s) 2411
BssSI CACGAG 2 cut(s) 1404, 2367
BssT1I CCWWGG 6 cut(s) 169, 1477, 1795, 2014, 2089, 2439
Bst2BI CACGAG 2 cut(s) 1404, 2367
Bst2UI CCWGG 3 cut(s) 243, 287, 1997
Bst4CI ACNGT 8 cut(s) 820, 1253, 1369, 1896, 2276, 2348, 2992, 3043
Bst6I CTCTTC 2 cut(s) 501, 1366
BstAFI CTTAAG 1 cut(s) 248
BstC8I GCNNGC 7 cut(s) 234, 1357, 1468, 1556, 2413, 2897, 2969
BstDEI CTNAG 6 cut(s) 296, 383, 1089, 1628, 2110, 2891
BstDSI CCRYGG 2 cut(s) 809, 2439
BstEII GGTNACC 1 cut(s) 2386
BstFNI CGCG 2 cut(s) 2411, 2413
BstH2I RGCGCY 1 cut(s) 1097
BstHHI GCGC 3 cut(s) 1096, 2413, 2415
BstMAI GTCTC 5 cut(s) 425, 830, 1773, 2074, 2933
BstMCI CGRYCG 1 cut(s) 2770
BstMWI GCNNNNNNNGC 8 cut(s) 601, 1272, 1467, 1832, 2030, 2419, 2927, 3032
BstNI CCWGG 3 cut(s) 243, 287, 1997
BstNSI RCATGY 4 cut(s) 236, 1147, 1359, 2925
BstPAI GACNNNNGTC 1 cut(s) 2098
BstPI GGTNACC 1 cut(s) 2386
BstSCI CCNGG 8 cut(s) 241, 285, 1995, 2239, 2240, 2257, 3086, 3087
BstSFI CTRYAG 2 cut(s) 1023, 3039
BstUI CGCG 2 cut(s) 2411, 2413
BstV1I GCAGC 6 cut(s) 613, 762, 787, 1054, 1147, 3038
BstV2I GAAGAC 1 cut(s) 97
BstX2I RGATCY 1 cut(s) 97
BstXI CCANNNNNNTGG 1 cut(s) 1322
BstYI RGATCY 1 cut(s) 97
Bsu15I ATCGAT 1 cut(s) 1971
Bsu36I CCTNAGG 1 cut(s) 1628
BsuI GTATCC 1 cut(s) 1843
BsuRI GGCC 6 cut(s) 329, 662, 721, 1601, 1872, 2472
BsuTUI ATCGAT 1 cut(s) 1971
BtgI CCRYGG 2 cut(s) 809, 2439
BtgZI GCGATG 2 cut(s) 2538, 3161
BtsIMutI CAGTG 5 cut(s) 771, 1892, 2387, 2461, 2997
Cac8I GCNNGC 7 cut(s) 234, 1357, 1468, 1556, 2413, 2897, 2969
CfoI GCGC 3 cut(s) 1096, 2413, 2415
Cfr10I RCCGGY 1 cut(s) 721
Cfr13I GGNCC 6 cut(s) 8, 509, 2093, 2261, 2679, 2720
Cfr9I CCCGGG 2 cut(s) 2240, 3087
ClaI ATCGAT 1 cut(s) 1971
CseI GACGC 1 cut(s) 601
CsiI ACCWGGT 2 cut(s) 241, 285
Csp6I GTAC 4 cut(s) 34, 1606, 2699, 2804
CspCI CAANNNNNGTGG 2 cut(s) 1354, 1389
CviQI GTAC 4 cut(s) 34, 1606, 2699, 2804
DdeI CTNAG 6 cut(s) 296, 383, 1089, 1628, 2110, 2891
DraI TTTAAA 1 cut(s) 306
EaeI YGGCCR 3 cut(s) 327, 719, 1870
Eam1104I CTCTTC 2 cut(s) 501, 1366
EarI CTCTTC 2 cut(s) 501, 1366
EciI GGCGGA 3 cut(s) 1019, 2025, 2261
Ecl136II GAGCTC 1 cut(s) 2461
Eco130I CCWWGG 6 cut(s) 169, 1477, 1795, 2014, 2089, 2439
Eco147I AGGCCT 1 cut(s) 2472
Eco24I GRGCYC 4 cut(s) 1405, 1921, 2463, 2871
Eco31I GGTCTC 1 cut(s) 830
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 6 cut(s) 8, 509, 2093, 2261, 2679, 2720
Eco53kI GAGCTC 1 cut(s) 2461
Eco57I CTGAAG 1 cut(s) 488
Eco81I CCTNAGG 1 cut(s) 1628
Eco88I CYCGRG 2 cut(s) 2240, 3087
Eco91I GGTNACC 1 cut(s) 2386
EcoICRI GAGCTC 1 cut(s) 2461
EcoO109I RGGNCCY 4 cut(s) 8, 509, 2093, 2679
EcoO65I GGTNACC 1 cut(s) 2386
EcoRII CCWGG 3 cut(s) 241, 285, 1995
EcoRV GATATC 1 cut(s) 415
EcoT14I CCWWGG 6 cut(s) 169, 1477, 1795, 2014, 2089, 2439
EcoT38I GRGCYC 4 cut(s) 1405, 1921, 2463, 2871
ErhI CCWWGG 6 cut(s) 169, 1477, 1795, 2014, 2089, 2439
Esp3I CGTCTC 1 cut(s) 425
FalI AAGNNNNNCTT 6 cut(s) 175, 207, 2497, 2529, 2624, 2656
FaqI GGGAC 3 cut(s) 558, 2032, 2833
FauI CCCGC 1 cut(s) 1369
FauNDI CATATG 2 cut(s) 370, 2973
FbaI TGATCA 2 cut(s) 1734, 3127
Fnu4HI GCNGC 8 cut(s) 602, 776, 801, 1033, 1068, 1161, 2986, 3027
FriOI GRGCYC 4 cut(s) 1405, 1921, 2463, 2871
Fsp4HI GCNGC 8 cut(s) 602, 776, 801, 1033, 1068, 1161, 2986, 3027
FspBI CTAG 3 cut(s) 1617, 2480, 3051
GlaI GCGC 3 cut(s) 1095, 2412, 2414
GluI GCNGC 8 cut(s) 602, 776, 801, 1033, 1068, 1161, 2986, 3027
GsaI CCCAGC 1 cut(s) 1918
GsuI CTGGAG 2 cut(s) 136, 2990
HaeII RGCGCY 1 cut(s) 1097
HaeIII GGCC 6 cut(s) 329, 662, 721, 1601, 1872, 2472
HapII CCGG 6 cut(s) 722, 876, 2241, 2258, 2799, 3088
HgaI GACGC 1 cut(s) 601
HhaI GCGC 3 cut(s) 1096, 2413, 2415
Hin6I GCGC 3 cut(s) 1094, 2411, 2413
HinP1I GCGC 3 cut(s) 1094, 2411, 2413
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HindIII AAGCTT 2 cut(s) 1699, 2655
HinfI GANTC 8 cut(s) 162, 377, 784, 1520, 1541, 2000, 2082, 2114
HpaI GTTAAC 1 cut(s) 148
HpaII CCGG 6 cut(s) 722, 876, 2241, 2258, 2799, 3088
HphI GGTGA 9 cut(s) 236, 926, 1410, 1421, 1777, 2077, 2380, 2858, 2870
Hpy166II GTNNAC 6 cut(s) 148, 292, 1249, 1606, 1644, 1768
Hpy188III TCNNGA 6 cut(s) 404, 1195, 1617, 1627, 2311, 2367
Hpy8I GTNNAC 6 cut(s) 148, 292, 1249, 1606, 1644, 1768
Hpy99I CGWCG 1 cut(s) 1554
HpyAV CCTTC 5 cut(s) 136, 189, 939, 1756, 3082
HpyCH4III ACNGT 8 cut(s) 820, 1253, 1369, 1896, 2276, 2348, 2992, 3043
HpyCH4IV ACGT 3 cut(s) 582, 1049, 1770
HpyF10VI GCNNNNNNNGC 8 cut(s) 601, 1272, 1467, 1832, 2030, 2419, 2927, 3032
HpyF3I CTNAG 6 cut(s) 296, 383, 1089, 1628, 2110, 2891
HpySE526I ACGT 3 cut(s) 582, 1049, 1770
HspAI GCGC 3 cut(s) 1094, 2411, 2413
KpnI GGTACC 1 cut(s) 37
Ksp22I TGATCA 2 cut(s) 1734, 3127
KspAI GTTAAC 1 cut(s) 148
LguI GCTCTTC 1 cut(s) 1366
LmnI GCTCC 5 cut(s) 253, 1502, 2038, 2271, 2466
Lsp1109I GCAGC 6 cut(s) 613, 762, 787, 1054, 1147, 3038
LweI GCATC 6 cut(s) 906, 1044, 2064, 2631, 2656, 2785
MabI ACCWGGT 2 cut(s) 241, 285
MaeI CTAG 3 cut(s) 1617, 2480, 3051
MaeII ACGT 3 cut(s) 582, 1049, 1770
MaeIII GTNAC 8 cut(s) 224, 515, 850, 1742, 2227, 2386, 2534, 2858
MbiI CCGCTC 1 cut(s) 2266
MfeI CAATTG 1 cut(s) 3095
MflI RGATCY 1 cut(s) 97
MhlI GDGCHC 7 cut(s) 58, 921, 1405, 1921, 2013, 2463, 2871
MlsI TGGCCA 2 cut(s) 329, 1872
MluNI TGGCCA 2 cut(s) 329, 1872
MlyI GAGTC 2 cut(s) 1550, 2076
MmeI TCCRAC 4 cut(s) 603, 1827, 2698, 2888
Mox20I TGGCCA 2 cut(s) 329, 1872
MroXI GAANNNNTTC 3 cut(s) 178, 2636, 3071
MscI TGGCCA 2 cut(s) 329, 1872
MseI TTAA 9 cut(s) 147, 249, 275, 305, 686, 869, 2424, 2504, 2762
MslI CAYNNNNRTG 3 cut(s) 839, 963, 1040
Msp20I TGGCCA 2 cut(s) 329, 1872
MspA1I CMGCKG 1 cut(s) 1160
MspCI CTTAAG 1 cut(s) 248
MspI CCGG 6 cut(s) 722, 876, 2241, 2258, 2799, 3088
MspR9I CCNGG 8 cut(s) 243, 287, 1997, 2241, 2242, 2259, 3088, 3089
MunI CAATTG 1 cut(s) 3095
Mva1269I GAATGC 1 cut(s) 1275
MvaI CCWGG 3 cut(s) 243, 287, 1997
MvnI CGCG 2 cut(s) 2411, 2413
MwoI GCNNNNNNNGC 8 cut(s) 601, 1272, 1467, 1832, 2030, 2419, 2927, 3032
NciI CCSGG 5 cut(s) 2241, 2242, 2259, 3088, 3089
NcoI CCATGG 1 cut(s) 2439
NdeI CATATG 2 cut(s) 370, 2973
NlaIV GGNNCC 9 cut(s) 9, 35, 510, 1498, 1573, 2095, 2183, 2262, 2445
NmeAIII GCCGAG 1 cut(s) 1473
NmuCI GTSAC 6 cut(s) 224, 850, 2227, 2386, 2534, 2858
NspI RCATGY 4 cut(s) 236, 1147, 1359, 2925
PaeI GCATGC 2 cut(s) 236, 1359
PauI GCGCGC 1 cut(s) 2411
PceI AGGCCT 1 cut(s) 2472
PciI ACATGT 1 cut(s) 1143
PciSI GCTCTTC 1 cut(s) 1366
PctI GAATGC 1 cut(s) 1275
PdmI GAANNNNTTC 3 cut(s) 178, 2636, 3071
PfeI GAWTC 6 cut(s) 162, 377, 784, 1520, 2000, 2114
PflFI GACNNNGTC 1 cut(s) 2683
PflMI CCANNNNNTGG 2 cut(s) 47, 911
PkrI GCNGC 8 cut(s) 603, 777, 802, 1034, 1069, 1162, 2987, 3028
PleI GAGTC 2 cut(s) 1549, 2076
PpsI GAGTC 2 cut(s) 1549, 2076
PpuMI RGGWCCY 4 cut(s) 8, 509, 2093, 2679
PscI ACATGT 1 cut(s) 1143
PshAI GACNNNNGTC 1 cut(s) 2098
Psp124BI GAGCTC 1 cut(s) 2463
Psp5II RGGWCCY 4 cut(s) 8, 509, 2093, 2679
Psp6I CCWGG 3 cut(s) 241, 285, 1995
PspEI GGTNACC 1 cut(s) 2386
PspFI CCCAGC 1 cut(s) 1914
PspGI CCWGG 3 cut(s) 241, 285, 1995
PspN4I GGNNCC 9 cut(s) 9, 35, 510, 1498, 1573, 2095, 2183, 2262, 2445
PspPI GGNCC 6 cut(s) 8, 509, 2093, 2261, 2679, 2720
PspPPI RGGWCCY 4 cut(s) 8, 509, 2093, 2679
PsuI RGATCY 1 cut(s) 97
PsyI GACNNNGTC 1 cut(s) 2683
PteI GCGCGC 1 cut(s) 2411
PvuII CAGCTG 1 cut(s) 1160
RsaI GTAC 4 cut(s) 35, 1607, 2700, 2805
RsaNI GTAC 4 cut(s) 34, 1606, 2699, 2804
RseI CAYNNNNRTG 3 cut(s) 839, 963, 1040
SacI GAGCTC 1 cut(s) 2463
SapI GCTCTTC 1 cut(s) 1366
SaqAI TTAA 9 cut(s) 147, 249, 275, 305, 686, 869, 2424, 2504, 2762
SatI GCNGC 8 cut(s) 602, 776, 801, 1033, 1068, 1161, 2986, 3027
Sau96I GGNCC 6 cut(s) 8, 509, 2093, 2261, 2679, 2720
SchI GAGTC 2 cut(s) 1550, 2076
ScrFI CCNGG 8 cut(s) 243, 287, 1997, 2241, 2242, 2259, 3088, 3089
SduI GDGCHC 7 cut(s) 58, 921, 1405, 1921, 2013, 2463, 2871
SexAI ACCWGGT 2 cut(s) 241, 285
SfaNI GCATC 6 cut(s) 906, 1044, 2064, 2631, 2656, 2785
SfcI CTRYAG 2 cut(s) 1023, 3039
SinI GGWCC 6 cut(s) 8, 509, 2093, 2261, 2679, 2720
SmaI CCCGGG 2 cut(s) 2242, 3089
SmiMI CAYNNNNRTG 3 cut(s) 839, 963, 1040
SmlI CTYRAG 3 cut(s) 248, 1058, 1538
SmoI CTYRAG 3 cut(s) 248, 1058, 1538
SpeI ACTAGT 1 cut(s) 3050
SphI GCATGC 2 cut(s) 236, 1359
SseBI AGGCCT 1 cut(s) 2472
SspI AATATT 4 cut(s) 269, 1104, 2204, 2497
SspMI CTAG 3 cut(s) 1617, 2480, 3051
SstI GAGCTC 1 cut(s) 2463
StuI AGGCCT 1 cut(s) 2472
StyD4I CCNGG 8 cut(s) 241, 285, 1995, 2239, 2240, 2257, 3086, 3087
StyI CCWWGG 6 cut(s) 169, 1477, 1795, 2014, 2089, 2439
TaaI ACNGT 8 cut(s) 820, 1253, 1369, 1896, 2276, 2348, 2992, 3043
TaiI ACGT 3 cut(s) 585, 1052, 1773
TaqI TCGA 5 cut(s) 441, 1971, 2049, 2865, 2926
TaqII GACCGA 2 cut(s) 663, 812
TatI WGTACW 2 cut(s) 2698, 2803
TauI GCSGC 2 cut(s) 1035, 2988
TfiI GAWTC 6 cut(s) 162, 377, 784, 1520, 2000, 2114
Tru1I TTAA 9 cut(s) 147, 249, 275, 305, 686, 869, 2424, 2504, 2762
Tru9I TTAA 9 cut(s) 147, 249, 275, 305, 686, 869, 2424, 2504, 2762
TscAI CASTG 5 cut(s) 771, 1899, 2387, 2461, 2997
TseFI GTSAC 6 cut(s) 224, 850, 2227, 2386, 2534, 2858
TseI GCWGC 6 cut(s) 601, 775, 800, 1067, 1160, 3026
Tsp45I GTSAC 6 cut(s) 224, 850, 2227, 2386, 2534, 2858
TspDTI ATGAA 9 cut(s) 109, 387, 1268, 1339, 1469, 1977, 2900, 3123, 3164
TspGWI ACGGA 2 cut(s) 407, 953
TspMI CCCGGG 2 cut(s) 2240, 3087
TspRI CASTG 5 cut(s) 771, 1899, 2387, 2461, 2997
Tth111I GACNNNGTC 1 cut(s) 2683
Van91I CCANNNNNTGG 2 cut(s) 47, 911
Vha464I CTTAAG 1 cut(s) 248
VpaK11BI GGWCC 6 cut(s) 8, 509, 2093, 2261, 2679, 2720
XapI RAATTY 6 cut(s) 744, 1425, 2122, 2838, 2933, 2999
XbaI TCTAGA 1 cut(s) 1616
XceI RCATGY 4 cut(s) 236, 1147, 1359, 2925
XmaI CCCGGG 2 cut(s) 2240, 3087
XmnI GAANNNNTTC 3 cut(s) 178, 2636, 3071
XspI CTAG 3 cut(s) 1617, 2480, 3051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.