pycom11g25120

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010527
Physical Location & Seq
Forward (+)
27984577 .. 27985722
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 972 bp
ATGCTTATGATGAGGGACTTCCCTTCTTGTTTTGGTGAAAATGGTGTTCAAGTTGCAGATTTTTCATCATCATCTTCTTCCTCAAGAGCCACAAAAGCTGCCCAGAACTTGGTTTCCTGCGTCTACCAGTGCAAATTAAAGGGCAGATTGTGTTTGATCACTGTGACATGGACCAAGAATCTGATGGGGCAAGGCCTTGCCATTGAAATCGACGATGCAGCCAGTCATTGCCTCTGCAGAGTTGAGATTAAGCCATGGATTTTCTCCAAGAGAAAAGGGTTGAAGAATTTGGAGGTGGACTCTACGAAAATTGACATCTTTTGGGATTTAACAAATGCCAAGTTTGGTTCAGGACCTGAACCATTGGAGAAGTTTTATCTAGCTCTTATGTTTGATCAAGAAATTGTTCTGTTTCTTGGGGATTTGAAGAGGGAGGATTTCAAAAGCCCTGCTGGGTCCAATTCCAAGTCCAAATCCAATTCCAATTCCAAATCCAAATCCACAGCCGAATTCGTTGCGAAAAGAGAGCACATTTGTGGGAAAAAAATTTATGGAGCAAAGGCACAGTTTTGTGATTTGGGCAAAACCCATGATGTGAAAATTGAGTGTGAAACTGCAGGTGGCCTCCATGAGGCATATCTTGTGATCTGCATTGACAGCAAGACTGTATTGCAGGTGAAGAGGTTGAATTGGAAGTTCAGAGGCAACCACACAATTGTGGTAGATGGGATGAGAGTTGAAGTGTTTTGGGATGTGCACAATTGGCTGTTTGGGAATTCAATGGGAGATGCAGTTTTCATGTTCCAAACTTGCCTCAATGTTGCTGCAGATGAAAGCAAGAAGTTATGGACTGGTTTGCCAGTTCTGGATCCTTCTGTGTTCACTTGGTCTTCTTCCTCTGAGCAGTTGAGAGAGAACCAGTTGCAGGATTTTGGGTTCTCACTGGTTTTGCATGCTTGGAAGAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

324

Amino Acids

36.29

Weight (kDa)

8.22

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 34 - 321 8.7e-100 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 608, 664
Acc36I ACCTGC 2 cut(s) 608, 664
AccB7I CCANNNNNTGG 1 cut(s) 109
AccI GTMKAC 1 cut(s) 123
AclWI GGATC 2 cut(s) 863, 876
AcsI RAATTY 4 cut(s) 286, 509, 546, 775
AfiI CCNNNNNNNGG 3 cut(s) 109, 631, 925
AgsI TTSAA 8 cut(s) 50, 206, 283, 427, 442, 688, 740, 780
AleI CACNNNNGTG 2 cut(s) 534, 716
AloI GAACNNNNNNTCC 4 cut(s) 98, 130, 920, 952
AluBI AGCT 2 cut(s) 98, 383
AluI AGCT 2 cut(s) 98, 383
Alw21I GWGCWC 2 cut(s) 531, 759
Alw44I GTGCAC 1 cut(s) 755
AlwI GGATC 2 cut(s) 863, 876
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 2 cut(s) 193, 622
ApaLI GTGCAC 1 cut(s) 755
ApeKI GCWGC 3 cut(s) 98, 218, 824
ApoI RAATTY 4 cut(s) 286, 509, 546, 775
Asp700I GAANNNNTTC 1 cut(s) 405
AspS9I GGNCC 3 cut(s) 171, 353, 456
AsuHPI GGTGA 2 cut(s) 47, 688
AvaII GGWCC 3 cut(s) 171, 353, 456
BaeGI GKGCMC 1 cut(s) 759
BamHI GGATCC 1 cut(s) 868
BbsI GAAGAC 1 cut(s) 882
Bbv12I GWGCWC 2 cut(s) 531, 759
BbvI GCAGC 3 cut(s) 85, 230, 811
BccI CCATC 2 cut(s) 178, 719
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BclI TGATCA 2 cut(s) 156, 394
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 3 cut(s) 235, 615, 825
BfuAI ACCTGC 2 cut(s) 608, 664
BisI GCNGC 3 cut(s) 99, 219, 825
BlsI GCNGC 3 cut(s) 100, 220, 826
Bme18I GGWCC 3 cut(s) 171, 353, 456
BmgT120I GGNCC 3 cut(s) 171, 353, 456
BmiI GGNNCC 2 cut(s) 457, 870
BmsI GCATC 2 cut(s) 205, 778
BpiI GAAGAC 1 cut(s) 882
BplI GAGNNNNNCTC 2 cut(s) 284, 316
BpuEI CTTGAG 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 254
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 631, 925
Bse1I ACTGG 6 cut(s) 127, 222, 856, 860, 919, 948
Bse3DI GCAATG 1 cut(s) 226
BseDI CCNNGG 1 cut(s) 254
BseGI GGATG 2 cut(s) 735, 757
BseLI CCNNNNNNNGG 3 cut(s) 109, 631, 925
BseMI GCAATG 1 cut(s) 226
BseMII CTCAG 1 cut(s) 891
BseNI ACTGG 6 cut(s) 127, 222, 856, 860, 919, 948
BseSI GKGCMC 1 cut(s) 759
BseXI GCAGC 3 cut(s) 85, 230, 811
BseYI CCCAGC 1 cut(s) 452
BshFI GGCC 2 cut(s) 195, 624
BsiHKAI GWGCWC 2 cut(s) 531, 759
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 3 cut(s) 109, 631, 925
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 2 cut(s) 195, 624
Bsp1286I GDGCHC 2 cut(s) 531, 759
Bsp143I GATC 4 cut(s) 156, 394, 645, 868
Bsp19I CCATGG 1 cut(s) 254
BspANI GGCC 2 cut(s) 195, 624
BspCNI CTCAG 1 cut(s) 892
BspLI GGNNCC 2 cut(s) 457, 870
BspMAI CTGCAG 3 cut(s) 239, 619, 829
BspMI ACCTGC 2 cut(s) 608, 664
BspPI GGATC 2 cut(s) 863, 876
BsrDI GCAATG 1 cut(s) 226
BsrI ACTGG 6 cut(s) 127, 222, 856, 860, 919, 948
BssECI CCNNGG 1 cut(s) 254
BssMI GATC 4 cut(s) 156, 394, 645, 868
BssT1I CCWWGG 1 cut(s) 254
Bst4CI ACNGT 3 cut(s) 163, 567, 667
Bst6I CTCTTC 2 cut(s) 422, 674
BstC8I GCNNGC 1 cut(s) 954
BstDEI CTNAG 1 cut(s) 900
BstDSI CCRYGG 1 cut(s) 254
BstENI CCTNNNNNAGG 1 cut(s) 629
BstF5I GGATG 2 cut(s) 735, 757
BstKTI GATC 4 cut(s) 159, 397, 648, 871
BstMBI GATC 4 cut(s) 156, 394, 645, 868
BstMWI GCNNNNNNNGC 3 cut(s) 95, 657, 763
BstNSI RCATGY 1 cut(s) 956
BstSFI CTRYAG 3 cut(s) 235, 615, 825
BstSLI GKGCMC 1 cut(s) 759
BstV1I GCAGC 3 cut(s) 85, 230, 811
BstV2I GAAGAC 1 cut(s) 882
BstX2I RGATCY 1 cut(s) 868
BstYI RGATCY 1 cut(s) 868
BsuRI GGCC 2 cut(s) 195, 624
BtgI CCRYGG 1 cut(s) 254
BtsCI GGATG 2 cut(s) 735, 757
BtsIMutI CAGTG 3 cut(s) 134, 159, 941
BveI ACCTGC 2 cut(s) 608, 664
Cac8I GCNNGC 1 cut(s) 954
CaiI CAGNNNCTG 1 cut(s) 356
Cfr13I GGNCC 3 cut(s) 171, 353, 456
CseI GACGC 1 cut(s) 109
CviAII CATG 6 cut(s) 168, 255, 590, 629, 799, 953
DdeI CTNAG 1 cut(s) 900
DpnI GATC 4 cut(s) 158, 396, 647, 870
DpnII GATC 4 cut(s) 156, 394, 645, 868
Eam1104I CTCTTC 2 cut(s) 422, 674
EarI CTCTTC 2 cut(s) 422, 674
Eco130I CCWWGG 1 cut(s) 254
Eco147I AGGCCT 1 cut(s) 195
Eco47I GGWCC 3 cut(s) 171, 353, 456
EcoNI CCTNNNNNAGG 1 cut(s) 629
EcoO109I RGGNCCY 1 cut(s) 353
EcoRI GAATTC 2 cut(s) 509, 775
EcoT14I CCWWGG 1 cut(s) 254
ErhI CCWWGG 1 cut(s) 254
FaeI CATG 6 cut(s) 171, 258, 593, 632, 802, 956
FaqI GGGAC 1 cut(s) 29
FatI CATG 6 cut(s) 167, 254, 589, 628, 798, 952
FbaI TGATCA 2 cut(s) 156, 394
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 99, 219, 825
FokI GGATG 2 cut(s) 742, 764
Fsp4HI GCNGC 3 cut(s) 99, 219, 825
FspBI CTAG 1 cut(s) 380
GluI GCNGC 3 cut(s) 99, 219, 825
GsaI CCCAGC 1 cut(s) 456
HaeIII GGCC 2 cut(s) 195, 624
HgaI GACGC 1 cut(s) 109
Hin1II CATG 6 cut(s) 171, 258, 593, 632, 802, 956
HinfI GANTC 2 cut(s) 178, 299
HphI GGTGA 2 cut(s) 47, 688
Hpy166II GTNNAC 4 cut(s) 124, 298, 757, 882
Hpy188I TCNGA 3 cut(s) 183, 701, 901
Hpy188III TCNNGA 4 cut(s) 84, 351, 398, 866
Hpy8I GTNNAC 4 cut(s) 124, 298, 757, 882
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 33, 882
HpyCH4III ACNGT 3 cut(s) 163, 567, 667
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 657, 763
HpyF3I CTNAG 1 cut(s) 900
Hsp92II CATG 6 cut(s) 171, 258, 593, 632, 802, 956
Ksp22I TGATCA 2 cut(s) 156, 394
Kzo9I GATC 4 cut(s) 156, 394, 645, 868
LmnI GCTCC 1 cut(s) 554
Lsp1109I GCAGC 3 cut(s) 85, 230, 811
LweI GCATC 2 cut(s) 205, 778
MaeI CTAG 1 cut(s) 380
MaeIII GTNAC 1 cut(s) 163
MalI GATC 4 cut(s) 158, 396, 647, 870
MboI GATC 4 cut(s) 156, 394, 645, 868
MboII GAAGA 7 cut(s) 66, 69, 295, 439, 691, 882, 885
MfeI CAATTG 2 cut(s) 714, 760
MflI RGATCY 1 cut(s) 868
MhlI GDGCHC 2 cut(s) 531, 759
MlyI GAGTC 1 cut(s) 293
MroXI GAANNNNTTC 1 cut(s) 405
MseI TTAA 3 cut(s) 137, 249, 329
MslI CAYNNNNRTG 2 cut(s) 534, 716
MunI CAATTG 2 cut(s) 714, 760
MwoI GCNNNNNNNGC 3 cut(s) 95, 657, 763
NcoI CCATGG 1 cut(s) 254
NdeII GATC 4 cut(s) 156, 394, 645, 868
NlaIII CATG 6 cut(s) 171, 258, 593, 632, 802, 956
NlaIV GGNNCC 2 cut(s) 457, 870
NmuCI GTSAC 1 cut(s) 163
NspI RCATGY 1 cut(s) 956
OliI CACNNNNGTG 2 cut(s) 534, 716
PaeI GCATGC 1 cut(s) 956
PaqCI CACCTGC 2 cut(s) 608, 664
PceI AGGCCT 1 cut(s) 195
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 109
PkrI GCNGC 3 cut(s) 100, 220, 826
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
PpuMI RGGWCCY 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 353
PspFI CCCAGC 1 cut(s) 452
PspN4I GGNNCC 2 cut(s) 457, 870
PspPI GGNCC 3 cut(s) 171, 353, 456
PspPPI RGGWCCY 1 cut(s) 353
PstI CTGCAG 3 cut(s) 239, 619, 829
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 1 cut(s) 868
RseI CAYNNNNRTG 2 cut(s) 534, 716
SaqAI TTAA 3 cut(s) 137, 249, 329
SatI GCNGC 3 cut(s) 99, 219, 825
Sau3AI GATC 4 cut(s) 156, 394, 645, 868
Sau96I GGNCC 3 cut(s) 171, 353, 456
SchI GAGTC 1 cut(s) 293
SduI GDGCHC 2 cut(s) 531, 759
SetI ASST 7 cut(s) 100, 297, 358, 385, 622, 678, 686
SfaNI GCATC 2 cut(s) 205, 778
SfcI CTRYAG 3 cut(s) 235, 615, 825
SinI GGWCC 3 cut(s) 171, 353, 456
SmiMI CAYNNNNRTG 2 cut(s) 534, 716
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
SphI GCATGC 1 cut(s) 956
SseBI AGGCCT 1 cut(s) 195
SspMI CTAG 1 cut(s) 380
StuI AGGCCT 1 cut(s) 195
StyI CCWWGG 1 cut(s) 254
TaaI ACNGT 3 cut(s) 163, 567, 667
TaqI TCGA 1 cut(s) 210
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 3 cut(s) 137, 249, 329
Tru9I TTAA 3 cut(s) 137, 249, 329
TscAI CASTG 3 cut(s) 134, 166, 948
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 3 cut(s) 98, 218, 824
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 3 cut(s) 54, 787, 846
TspRI CASTG 3 cut(s) 134, 166, 948
Van91I CCANNNNNTGG 1 cut(s) 109
VneI GTGCAC 1 cut(s) 755
VpaK11BI GGWCC 3 cut(s) 171, 353, 456
XagI CCTNNNNNAGG 1 cut(s) 629
XapI RAATTY 4 cut(s) 286, 509, 546, 775
XceI RCATGY 1 cut(s) 956
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 123
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.