pycom11g25430
BHLH Family

basic helix-loop-helix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010418
Physical Location & Seq
Reverse (-)
28252760 .. 28259591
6832 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2130 bp
ATGGCTGCACCGCCGCCAAGCAGCAGCCGCCTCCGTGGTATGTTGCAGGCCTCAGTCCAATATGTCCAATGGACTTACAGTCTCTTCTGGCAAATCTGTCCCCAACAAGGGATCTTAGTATGGTCAGATGGGTACTATAATGGAGCCATCAAGACGAGGAAGACGGTGCAACCAATGGAAGTGAGTGCCGAGGAGGCATCTCTCCAGAGGAGCCAGCAACTCAGAGAACTCTACGACTCTTTGTCCGCTGGAGAGACAAACCAGCCCCCAGCACGCCGCCCTTGCGCTTCCTTGTCCCCGGAGGACTTAACCGAATCCGAATGGTTCTACTTGATGTGTGTCTCATTCTCCTTTCCCCCCGGCGTCGGGTTGCCAGGGAAAGCATACGCAAGGAGGCAGCATGTATGGCTCACCGGTGCAAACGAGGTCGATAGCAAAACCTTTTCCAGAGCTATTTTGGCAAAGAGTGCTCGTATACAGACCGTAGTGTGCATTCCTCTTCTAGATGGCGTCGTAGAATTTGGCACCACAGAGAGGGTTCCAGAAGACCACGCCTTAGTCGAACACGTCAAAACCTTCTTCGTTGACCACCACCACCCTCCGCCACCAAAACCCGCCCTCTCCGAGCACTCCACATCCAACCCCGCCACCTCATCCGATCACCCACATTTCCACTCTCCGCACCTTCTCCAGACCATGTGCACCAACCCTCCTCTCAACGCCGCCCAAGAAGACGAAGAGGACGAAGAAGAAGATGATAATCAGGAGGAGGACGACGGAGGAGACGAGTCCGACTCCGAAGCCGAAACGGGTCGCAATGGTGGAGCCGTTGTTCCCGCCGCAAACCCTCCTCAGGTTTTGGCCGCGGTAGCCGAGCCAAGCGAGCTCATGCAACTCGAGATGTCCGAAGACATCCGGCTGGGCTCCCCGGACGATGCCTCAAATAACTTGGACTCTGATTTCCACTTGTTAGCTGTGAGTCAGTCTAGGAACCCAGCGGATCAGCAGAGACAAGCTGACTCGTATCGAGCCGAGTCGACCAGGCGGTGTCCGTCAGTACAAGAGCCGCTGAGCAGTGGCCTTCAACCGCCGCACACAGGACCCCTAGCTTTAGAGGAGTTGACACATGATGACGACACACATTATTCGGAGACGGTCTCCACCATACTGCAGGGACAAGCGACTCGGTGGATGGATTCATCGTCCACCAACTACACAGCTTGCTTGACTCAGTCGGCTTTCGCCAAGTGGTCGAGCCGGGTTGATCACCACTTTCTCATGCCGGTTGAGGGCACGTCTCAATGGCTCTTGAAATATATTTTATTCAGTGTACCGTTCCTCCACTCAAAATATCGCGACGAAAACTCGCCAAAATCTCAAGAGGGCGAAGGCTCGACGCGGTTGAGGAAAGGGACCCCACAAGACGAGCTCAGTGCCAATCATGTGTTAGCGGAACGACGTCGTAGAGAGAAGCTTAATGAGAGGTTTATTATACTAAGGTCCCTAGTGCCTTTTGTGACAAAAATGGACAAGGCTTCGATATTAGGGGACACAATCGAGTATGTGAAGCAACTGCGTAACAAAATTCAGGATCTCGAGGCACGTAACATGCTGATGGAGGAAGATCAACGATCGAGATCATCCGGGGAAATGCAAAGGTCCAGTAGTTGTAAAGAGTTGCGAAGTGGGCTCACGGTAGTGGAGCGGACCCAAGGAGGTCCACCGGGGTCCGATAAAAGGAAGTTGAGGATTGTGGAGGGAAGCGGCGGTGTCGCCATTGGTAAGGCTAAAGTAATGGAGGACTCACCGCCTCCACCGCCCCCGCCACCACCTCAGCCAGAACCTTCACCGACACCTATGGTGACGGGGACTTCTCTAGAGGTGTCGATAATCGAGAGTGATGGGCTGTTGGAGCTCCAATGCCCGTATAGAGAAGGGTTATTGCTTGATGTGATGCAAACACTTAGGGAGCTAAGAATTGAGACCACGGTGGTCCAGTCCTCATTGAATAACGGATTCTTCGTAGCTGAACTAAGAGCCAAGGTGAAGGATAACGTGAGTGGCAAGAAAGTAAGTATTACGGAAGTGAAGAGGGTGATAAATCAAATTATACCCCAATCTGACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

710

Amino Acids

78.65

Weight (kDa)

5.17

Isoelectric Point (pI)

68.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 11 - 194 1e-51 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 479 - 526 7.2e-13 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 626 - 704 1.5e-15 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1462
AccB1I GGYRCC 1 cut(s) 524
AccBSI CCGCTC 1 cut(s) 1705
AccI GTMKAC 2 cut(s) 475, 1037
AccII CGCG 3 cut(s) 866, 1356, 1399
AclWI GGATC 3 cut(s) 119, 1008, 1599
AcoI YGGCCR 1 cut(s) 861
AcsI RAATTY 2 cut(s) 518, 1584
AcyI GRCGYC 3 cut(s) 363, 510, 1459
AfaI GTAC 3 cut(s) 134, 1059, 1332
AfiI CCNNNNNNNGG 9 cut(s) 107, 108, 365, 366, 534, 853, 1097, 1289, 1737
AflIII ACRYGT 1 cut(s) 565
AgeI ACCGGT 1 cut(s) 413
AgsI TTSAA 3 cut(s) 1085, 1312, 2008
AhdI GACNNNNNGTC 2 cut(s) 78, 241
AjiI CACGTC 2 cut(s) 568, 1296
AjnI CCWGG 2 cut(s) 373, 1040
AleI CACNNNNGTG 1 cut(s) 1697
AloI GAACNNNNNNTCC 2 cut(s) 816, 848
Alw21I GWGCWC 6 cut(s) 472, 630, 704, 888, 1431, 1917
Alw26I GTCTC 9 cut(s) 86, 248, 346, 777, 1003, 1145, 1162, 1302, 1976
Alw44I GTGCAC 1 cut(s) 700
AlwI GGATC 3 cut(s) 119, 1008, 1599
Ama87I CYCGRG 2 cut(s) 896, 1595
AoxI GGCC 3 cut(s) 48, 861, 1078
ApaLI GTGCAC 1 cut(s) 700
ApeKI GCWGC 4 cut(s) 5, 21, 24, 397
ApoI RAATTY 2 cut(s) 518, 1584
AsiGI ACCGGT 1 cut(s) 413
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 8 cut(s) 1100, 1413, 1500, 1659, 1707, 1718, 1728, 1993
AsuC2I CCSGG 6 cut(s) 299, 360, 929, 1259, 1645, 1725
AsuHPI GGTGA 8 cut(s) 403, 653, 1259, 1797, 1839, 1873, 2056, 2107
AvaI CYCGRG 2 cut(s) 896, 1595
AvaII GGWCC 8 cut(s) 1100, 1413, 1500, 1659, 1707, 1718, 1728, 1993
AxyI CCTNAGG 1 cut(s) 852
BaeGI GKGCMC 2 cut(s) 704, 1295
BanI GGYRCC 1 cut(s) 524
BanII GRGCYC 5 cut(s) 888, 926, 1431, 1692, 1917
BbsI GAAGAC 4 cut(s) 167, 552, 738, 915
Bbv12I GWGCWC 6 cut(s) 472, 630, 704, 888, 1431, 1917
BbvCI CCTCAGC 1 cut(s) 1833
BbvI GCAGC 3 cut(s) 33, 36, 409
BccI CCATC 6 cut(s) 122, 155, 500, 1186, 1609, 1895
BceAI ACGGC 1 cut(s) 812
BcgI CGANNNNNNTGC 2 cut(s) 1415, 1449
BciT130I CCWGG 2 cut(s) 375, 1042
BclI TGATCA 1 cut(s) 1264
BcnI CCSGG 6 cut(s) 299, 360, 929, 1259, 1645, 1725
BcoDI GTCTC 9 cut(s) 86, 248, 346, 777, 1003, 1145, 1162, 1302, 1976
BfaI CTAG 5 cut(s) 503, 987, 1106, 1505, 1877
BfmI CTRYAG 1 cut(s) 1169
BglI GCCNNNNNGGC 1 cut(s) 194
BlpI GCTNAGC 1 cut(s) 1070
Bme1390I CCNGG 8 cut(s) 299, 360, 375, 929, 1042, 1259, 1645, 1725
Bme18I GGWCC 8 cut(s) 1100, 1413, 1500, 1659, 1707, 1718, 1728, 1993
BmeRI GACNNNNNGTC 2 cut(s) 78, 241
BmeT110I CYCGRG 2 cut(s) 896, 1595
BmgBI CACGTC 2 cut(s) 568, 1296
BmgT120I GGNCC 8 cut(s) 1100, 1413, 1500, 1659, 1707, 1718, 1728, 1993
BmrFI CCNGG 8 cut(s) 299, 360, 375, 929, 1042, 1259, 1645, 1725
BmsI GCATC 3 cut(s) 206, 925, 1944
BpiI GAAGAC 4 cut(s) 167, 552, 738, 915
BplI GAGNNNNNCTC 4 cut(s) 779, 811, 1142, 1174
BpmI CTGGAG 3 cut(s) 188, 270, 674
Bpu10I CCTNAGC 1 cut(s) 1833
Bpu1102I GCTNAGC 1 cut(s) 1070
BpuEI CTTGAG 1 cut(s) 1362
BpuMI CCSGG 6 cut(s) 299, 360, 929, 1259, 1645, 1725
BsaAI YACGTR 1 cut(s) 1604
BsaBI GATNNNNATC 2 cut(s) 759, 1636
BsaHI GRCGYC 3 cut(s) 363, 510, 1459
BsaI GGTCTC 2 cut(s) 1162, 1976
BsaWI WCCGGW 1 cut(s) 413
BsaXI ACNNNNNCTCC 6 cut(s) 694, 724, 816, 846, 1323, 1353
Bsc4I CCNNNNNNNGG 9 cut(s) 107, 108, 365, 366, 534, 853, 1097, 1289, 1737
Bse118I RCCGGY 2 cut(s) 413, 1282
Bse1I ACTGG 2 cut(s) 1662, 1996
Bse21I CCTNAGG 1 cut(s) 852
Bse3DI GCAATG 1 cut(s) 823
Bse8I GATNNNNATC 2 cut(s) 759, 1636
BseBI CCWGG 2 cut(s) 375, 1042
BseGI GGATG 5 cut(s) 635, 653, 912, 1197, 1640
BseJI GATNNNNATC 2 cut(s) 759, 1636
BseLI CCNNNNNNNGG 9 cut(s) 107, 108, 365, 366, 534, 853, 1097, 1289, 1737
BseMI GCAATG 1 cut(s) 823
BseMII CTCAG 7 cut(s) 66, 235, 866, 1061, 1244, 1444, 1847
BseNI ACTGG 2 cut(s) 1662, 1996
BseRI GAGGAG 7 cut(s) 206, 223, 702, 782, 795, 840, 1130
BseSI GKGCMC 2 cut(s) 704, 1295
BseXI GCAGC 3 cut(s) 33, 36, 409
BseYI CCCAGC 3 cut(s) 268, 919, 994
Bsh1236I CGCG 3 cut(s) 866, 1356, 1399
Bsh1285I CGRYCG 1 cut(s) 1634
BshFI GGCC 3 cut(s) 50, 863, 1080
BshNI GGYRCC 1 cut(s) 524
BshTI ACCGGT 1 cut(s) 413
BsiEI CGRYCG 1 cut(s) 1634
BsiHKAI GWGCWC 6 cut(s) 472, 630, 704, 888, 1431, 1917
BsiHKCI CYCGRG 2 cut(s) 896, 1595
BsiSI CCGG 9 cut(s) 299, 360, 414, 916, 929, 1258, 1283, 1644, 1724
BslFI GGGAC 7 cut(s) 84, 280, 1188, 1426, 1486, 1562, 1882
BslI CCNNNNNNNGG 9 cut(s) 107, 108, 365, 366, 534, 853, 1097, 1289, 1737
BsmAI GTCTC 9 cut(s) 86, 248, 346, 777, 1003, 1145, 1162, 1302, 1976
BsmBI CGTCTC 3 cut(s) 777, 1145, 1302
BsmFI GGGAC 7 cut(s) 84, 280, 1188, 1426, 1486, 1562, 1882
BsmI GAATGC 1 cut(s) 492
BsnI GGCC 3 cut(s) 50, 863, 1080
Bso31I GGTCTC 2 cut(s) 1162, 1976
BsoBI CYCGRG 2 cut(s) 896, 1595
Bsp1286I GDGCHC 9 cut(s) 472, 630, 704, 888, 926, 1295, 1431, 1692, 1917
Bsp143I GATC 8 cut(s) 111, 658, 1000, 1264, 1591, 1624, 1631, 1637
Bsp1720I GCTNAGC 1 cut(s) 1070
Bsp68I TCGCGA 1 cut(s) 1356
BspANI GGCC 3 cut(s) 50, 863, 1080
BspCNI CTCAG 7 cut(s) 65, 234, 865, 1062, 1243, 1443, 1846
BspFNI CGCG 3 cut(s) 866, 1356, 1399
BspMAI CTGCAG 1 cut(s) 1173
BspPI GGATC 3 cut(s) 119, 1008, 1599
BspT107I GGYRCC 1 cut(s) 524
BspTNI GGTCTC 2 cut(s) 1162, 1976
BsrBI CCGCTC 1 cut(s) 1705
BsrDI GCAATG 1 cut(s) 823
BsrFI RCCGGY 2 cut(s) 413, 1282
BsrI ACTGG 2 cut(s) 1662, 1996
BssAI RCCGGY 2 cut(s) 413, 1282
BssMI GATC 8 cut(s) 111, 658, 1000, 1264, 1591, 1624, 1631, 1637
BssNAI GTATAC 1 cut(s) 476
BssNI GRCGYC 3 cut(s) 363, 510, 1459
BssT1I CCWWGG 2 cut(s) 1711, 2040
Bst1107I GTATAC 1 cut(s) 476
Bst2UI CCWGG 2 cut(s) 375, 1042
Bst4CI ACNGT 7 cut(s) 80, 166, 484, 1156, 1335, 1696, 1990
Bst6I CTCTTC 4 cut(s) 89, 504, 732, 2084
BstACI GRCGYC 3 cut(s) 363, 510, 1459
BstAPI GCANNNNNTGC 1 cut(s) 467
BstBAI YACGTR 1 cut(s) 1604
BstC8I GCNNGC 5 cut(s) 48, 215, 274, 884, 1222
BstDSI CCRYGG 3 cut(s) 34, 864, 1986
BstF5I GGATG 5 cut(s) 635, 653, 912, 1197, 1640
BstFNI CGCG 3 cut(s) 866, 1356, 1399
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 8 cut(s) 114, 661, 1003, 1267, 1594, 1627, 1634, 1640
BstMAI GTCTC 9 cut(s) 86, 248, 346, 777, 1003, 1145, 1162, 1302, 1976
BstMBI GATC 8 cut(s) 111, 658, 1000, 1264, 1591, 1624, 1631, 1637
BstMCI CGRYCG 1 cut(s) 1634
BstNI CCWGG 2 cut(s) 375, 1042
BstNSI RCATGY 2 cut(s) 404, 1612
BstSCI CCNGG 8 cut(s) 297, 358, 373, 927, 1040, 1257, 1643, 1723
BstSFI CTRYAG 1 cut(s) 1169
BstSLI GKGCMC 2 cut(s) 704, 1295
BstUI CGCG 3 cut(s) 866, 1356, 1399
BstV1I GCAGC 3 cut(s) 33, 36, 409
BstV2I GAAGAC 4 cut(s) 167, 552, 738, 915
BstX2I RGATCY 2 cut(s) 111, 1591
BstYI RGATCY 2 cut(s) 111, 1591
BstZ17I GTATAC 1 cut(s) 476
Bsu36I CCTNAGG 1 cut(s) 852
BsuRI GGCC 3 cut(s) 50, 863, 1080
BtgI CCRYGG 3 cut(s) 34, 864, 1986
BtrI CACGTC 2 cut(s) 568, 1296
BtsCI GGATG 5 cut(s) 635, 653, 912, 1197, 1640
BtsI GCAGTG 1 cut(s) 1081
BtsIMutI CAGTG 3 cut(s) 1081, 1333, 1438
BtuMI TCGCGA 1 cut(s) 1356
Cac8I GCNNGC 5 cut(s) 48, 215, 274, 884, 1222
CfoI GCGC 1 cut(s) 287
Cfr10I RCCGGY 2 cut(s) 413, 1282
Cfr13I GGNCC 8 cut(s) 1100, 1413, 1500, 1659, 1707, 1718, 1728, 1993
Cfr42I CCGCGG 1 cut(s) 867
CseI GACGC 3 cut(s) 352, 499, 1405
Csp6I GTAC 3 cut(s) 133, 1058, 1331
CspAI ACCGGT 1 cut(s) 413
CviAII CATG 7 cut(s) 401, 697, 889, 1127, 1279, 1442, 1609
CviQI GTAC 3 cut(s) 133, 1058, 1331
DpnI GATC 8 cut(s) 113, 660, 1002, 1266, 1593, 1626, 1633, 1639
DpnII GATC 8 cut(s) 111, 658, 1000, 1264, 1591, 1624, 1631, 1637
DriI GACNNNNNGTC 2 cut(s) 78, 241
EaeI YGGCCR 1 cut(s) 861
Eam1104I CTCTTC 4 cut(s) 89, 504, 732, 2084
Eam1105I GACNNNNNGTC 2 cut(s) 78, 241
EarI CTCTTC 4 cut(s) 89, 504, 732, 2084
EciI GGCGGA 1 cut(s) 591
Ecl136II GAGCTC 3 cut(s) 886, 1429, 1915
Eco130I CCWWGG 2 cut(s) 1711, 2040
Eco147I AGGCCT 1 cut(s) 50
Eco24I GRGCYC 5 cut(s) 888, 926, 1431, 1692, 1917
Eco31I GGTCTC 2 cut(s) 1162, 1976
Eco47I GGWCC 8 cut(s) 1100, 1413, 1500, 1659, 1707, 1718, 1728, 1993
Eco53kI GAGCTC 3 cut(s) 886, 1429, 1915
Eco81I CCTNAGG 1 cut(s) 852
Eco88I CYCGRG 2 cut(s) 896, 1595
EcoICRI GAGCTC 3 cut(s) 886, 1429, 1915
EcoO109I RGGNCCY 3 cut(s) 1100, 1413, 1500
EcoRII CCWGG 2 cut(s) 373, 1040
EcoT14I CCWWGG 2 cut(s) 1711, 2040
EcoT38I GRGCYC 5 cut(s) 888, 926, 1431, 1692, 1917
ErhI CCWWGG 2 cut(s) 1711, 2040
Esp3I CGTCTC 3 cut(s) 777, 1145, 1302
FaeI CATG 7 cut(s) 404, 700, 892, 1130, 1282, 1445, 1612
FaqI GGGAC 7 cut(s) 84, 280, 1188, 1426, 1486, 1562, 1882
FatI CATG 7 cut(s) 400, 696, 888, 1126, 1278, 1441, 1608
FauI CCCGC 4 cut(s) 622, 652, 844, 1830
FbaI TGATCA 1 cut(s) 1264
FblI GTMKAC 2 cut(s) 475, 1037
FokI GGATG 5 cut(s) 622, 640, 899, 1204, 1627
FriOI GRGCYC 5 cut(s) 888, 926, 1431, 1692, 1917
FspBI CTAG 5 cut(s) 503, 987, 1106, 1505, 1877
GlaI GCGC 1 cut(s) 286
GsaI CCCAGC 3 cut(s) 272, 923, 998
GsuI CTGGAG 3 cut(s) 188, 270, 674
HaeIII GGCC 3 cut(s) 50, 863, 1080
HapII CCGG 9 cut(s) 299, 360, 414, 916, 929, 1258, 1283, 1644, 1724
HgaI GACGC 3 cut(s) 352, 499, 1405
HhaI GCGC 1 cut(s) 287
Hin1I GRCGYC 3 cut(s) 363, 510, 1459
Hin1II CATG 7 cut(s) 404, 700, 892, 1130, 1282, 1445, 1612
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HincII GTYRAC 3 cut(s) 586, 1038, 1122
HindII GTYRAC 3 cut(s) 586, 1038, 1122
HindIII AAGCTT 1 cut(s) 1472
HpaII CCGG 9 cut(s) 299, 360, 414, 916, 929, 1258, 1283, 1644, 1724
HphI GGTGA 8 cut(s) 403, 653, 1259, 1797, 1839, 1873, 2056, 2107
Hpy166II GTNNAC 8 cut(s) 476, 586, 702, 1038, 1122, 1206, 1331, 1721
Hpy8I GTNNAC 8 cut(s) 476, 586, 702, 1038, 1122, 1206, 1331, 1721
Hpy99I CGWCG 7 cut(s) 368, 515, 779, 1361, 1399, 1461, 1464
HpyAV CCTTC 7 cut(s) 586, 695, 1091, 1382, 1854, 1928, 2041
HpyCH4III ACNGT 7 cut(s) 80, 166, 484, 1156, 1335, 1696, 1990
HpyCH4IV ACGT 5 cut(s) 567, 1295, 1459, 1603, 2055
HpySE526I ACGT 5 cut(s) 567, 1295, 1459, 1603, 2055
Hsp92I GRCGYC 3 cut(s) 363, 510, 1459
Hsp92II CATG 7 cut(s) 404, 700, 892, 1130, 1282, 1445, 1612
HspAI GCGC 1 cut(s) 285
KflI GGGWCCC 1 cut(s) 1413
Ksp22I TGATCA 1 cut(s) 1264
KspI CCGCGG 1 cut(s) 867
Kzo9I GATC 8 cut(s) 111, 658, 1000, 1264, 1591, 1624, 1631, 1637
LmnI GCTCC 8 cut(s) 143, 210, 824, 929, 1702, 1912, 1920, 1969
Lsp1109I GCAGC 3 cut(s) 33, 36, 409
LweI GCATC 3 cut(s) 206, 925, 1944
MaeI CTAG 5 cut(s) 503, 987, 1106, 1505, 1877
MaeII ACGT 5 cut(s) 567, 1295, 1459, 1603, 2055
MaeIII GTNAC 4 cut(s) 1516, 1577, 1604, 1861
MalI GATC 8 cut(s) 113, 660, 1002, 1266, 1593, 1626, 1633, 1639
MbiI CCGCTC 1 cut(s) 1705
MboI GATC 8 cut(s) 111, 658, 1000, 1264, 1591, 1624, 1631, 1637
MflI RGATCY 2 cut(s) 111, 1591
MhlI GDGCHC 9 cut(s) 472, 630, 704, 888, 926, 1295, 1431, 1692, 1917
MluCI AATT 4 cut(s) 518, 1584, 1977, 2106
MmeI TCCRAC 3 cut(s) 663, 816, 1890
MseI TTAA 3 cut(s) 308, 1476, 2128
MslI CAYNNNNRTG 2 cut(s) 1613, 1697
MspA1I CMGCKG 4 cut(s) 248, 866, 998, 1069
MspI CCGG 9 cut(s) 299, 360, 414, 916, 929, 1258, 1283, 1644, 1724
MspR9I CCNGG 8 cut(s) 299, 360, 375, 929, 1042, 1259, 1645, 1725
Mva1269I GAATGC 1 cut(s) 492
MvaI CCWGG 2 cut(s) 375, 1042
MvnI CGCG 3 cut(s) 866, 1356, 1399
NciI CCSGG 6 cut(s) 299, 360, 929, 1259, 1645, 1725
NdeII GATC 8 cut(s) 111, 658, 1000, 1264, 1591, 1624, 1631, 1637
NlaIII CATG 7 cut(s) 404, 700, 892, 1130, 1282, 1445, 1612
NmeAIII GCCGAG 3 cut(s) 214, 898, 1057
NmuCI GTSAC 2 cut(s) 1516, 1861
NruI TCGCGA 1 cut(s) 1356
NspI RCATGY 2 cut(s) 404, 1612
OliI CACNNNNGTG 1 cut(s) 1697
PaeR7I CTCGAG 2 cut(s) 896, 1595
PceI AGGCCT 1 cut(s) 50
PcsI WCGNNNNNNNCGW 5 cut(s) 558, 741, 783, 903, 2019
PctI GAATGC 1 cut(s) 492
PfeI GAWTC 3 cut(s) 314, 1196, 2016
PflFI GACNNNGTC 1 cut(s) 1231
PinAI ACCGGT 1 cut(s) 413
Ple19I CGATCG 1 cut(s) 1634
Ppu21I YACGTR 1 cut(s) 1604
PpuMI RGGWCCY 3 cut(s) 1100, 1413, 1500
Psp124BI GAGCTC 3 cut(s) 888, 1431, 1917
Psp5II RGGWCCY 3 cut(s) 1100, 1413, 1500
Psp6I CCWGG 2 cut(s) 373, 1040
PspFI CCCAGC 3 cut(s) 268, 919, 994
PspGI CCWGG 2 cut(s) 373, 1040
PspPI GGNCC 8 cut(s) 1100, 1413, 1500, 1659, 1707, 1718, 1728, 1993
PspPPI RGGWCCY 3 cut(s) 1100, 1413, 1500
PstI CTGCAG 1 cut(s) 1173
PsuI RGATCY 2 cut(s) 111, 1591
PsyI GACNNNGTC 1 cut(s) 1231
PvuI CGATCG 1 cut(s) 1634
RruI TCGCGA 1 cut(s) 1356
RsaI GTAC 3 cut(s) 134, 1059, 1332
RsaNI GTAC 3 cut(s) 133, 1058, 1331
RseI CAYNNNNRTG 2 cut(s) 1613, 1697
SacI GAGCTC 3 cut(s) 888, 1431, 1917
SacII CCGCGG 1 cut(s) 867
SalI GTCGAC 1 cut(s) 1036
SaqAI TTAA 3 cut(s) 308, 1476, 2128
Sau3AI GATC 8 cut(s) 111, 658, 1000, 1264, 1591, 1624, 1631, 1637
Sau96I GGNCC 8 cut(s) 1100, 1413, 1500, 1659, 1707, 1718, 1728, 1993
ScrFI CCNGG 8 cut(s) 299, 360, 375, 929, 1042, 1259, 1645, 1725
SduI GDGCHC 9 cut(s) 472, 630, 704, 888, 926, 1295, 1431, 1692, 1917
SfaNI GCATC 3 cut(s) 206, 925, 1944
SfcI CTRYAG 1 cut(s) 1169
Sfr274I CTCGAG 2 cut(s) 896, 1595
Sfr303I CCGCGG 1 cut(s) 867
SgrAI CRCCGGYG 1 cut(s) 413
SgrBI CCGCGG 1 cut(s) 867
SinI GGWCC 8 cut(s) 1100, 1413, 1500, 1659, 1707, 1718, 1728, 1993
SlaI CTCGAG 2 cut(s) 896, 1595
SmiMI CAYNNNNRTG 2 cut(s) 1613, 1697
SmlI CTYRAG 3 cut(s) 896, 1377, 1595
SmoI CTYRAG 3 cut(s) 896, 1377, 1595
Sse9I AATT 4 cut(s) 518, 1584, 1977, 2106
SseBI AGGCCT 1 cut(s) 50
SspMI CTAG 5 cut(s) 503, 987, 1106, 1505, 1877
SstI GAGCTC 3 cut(s) 888, 1431, 1917
StuI AGGCCT 1 cut(s) 50
StyD4I CCNGG 8 cut(s) 297, 358, 373, 927, 1040, 1257, 1643, 1723
StyI CCWWGG 2 cut(s) 1711, 2040
TaaI ACNGT 7 cut(s) 80, 166, 484, 1156, 1335, 1696, 1990
TaiI ACGT 5 cut(s) 570, 1298, 1462, 1606, 2058
TasI AATT 4 cut(s) 518, 1584, 1977, 2106
TatI WGTACW 1 cut(s) 1057
TauI GCSGC 9 cut(s) 16, 30, 279, 725, 842, 866, 1069, 1093, 1767
TfiI GAWTC 3 cut(s) 314, 1196, 2016
Tru1I TTAA 3 cut(s) 308, 1476, 2128
Tru9I TTAA 3 cut(s) 308, 1476, 2128
TscAI CASTG 3 cut(s) 1081, 1333, 1438
TseFI GTSAC 2 cut(s) 1516, 1861
TseI GCWGC 4 cut(s) 5, 21, 24, 397
Tsp45I GTSAC 2 cut(s) 1516, 1861
TspDTI ATGAA 1 cut(s) 1188
TspGWI ACGGA 5 cut(s) 23, 792, 1041, 2028, 2096
TspRI CASTG 3 cut(s) 1081, 1333, 1438
Tth111I GACNNNGTC 1 cut(s) 1231
VneI GTGCAC 1 cut(s) 700
VpaK11BI GGWCC 8 cut(s) 1100, 1413, 1500, 1659, 1707, 1718, 1728, 1993
XapI RAATTY 2 cut(s) 518, 1584
XbaI TCTAGA 2 cut(s) 502, 1876
XceI RCATGY 2 cut(s) 404, 1612
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XhoI CTCGAG 2 cut(s) 896, 1595
XmiI GTMKAC 2 cut(s) 475, 1037
XspI CTAG 5 cut(s) 503, 987, 1106, 1505, 1877
ZraI GACGTC 1 cut(s) 1460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.