pycom11g25810

Mitochondrial ubiquitin ligase activator of NFKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011309
Physical Location & Seq
Forward (+)
28510896 .. 28513474
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1029 bp
ATGCTACCTTGGGGTGGATTGAGTTGTTGTCTGAGTGGAGCTGCTCTTTACCTTCTTGGCAGGAGCAGTGGCAGGGATGCAGAGATTCTTAAATCCGCCACTCGGATTAATCAGTTAAAGGAATTGGCAAAATTGTTGGATTCCGAATTTAGATTGCCAGTGGTGGTTGCAGTTTCGGGGAGAGTTAGCTCTGAAACCCCCATCAACTGTGAGTTCACTGGTTTACGAGGCGTAGTTGTGGAGGAAACGGCAGAACAACATTTTTTGAAACACAATGATGCTGGCTCATGGATACAGGATTCTGCTTTGATGCTTTCAATGAGTAAAGAAGTTCCCTGGTACTTGGATGATGGAACTGGTCGTGTGTTTGTGGTGGGAGCACGAGCTGCCACAGGATTTTTATTGCCGGTTGCAAGTGAGGTATTTGAAGAGTCAGGACGATCCCTCGTACGTGGGACATTAGATTATCTCCAAGGCCTCAAGATGCTTGGAGTCAAACGAATTGAGAGGGTACTTCCAACCGGTACTTCTATGAGTGTTGTTGGTGAGGCTGTCAAAGATGACATCGGTACCATTCGGATTCAACGACCTCAGAAGGGGCCATTTTATGTCTCTCCCAAAACCATTGATCAGCTTATTGCTAACCTTGGAAAATGGGCAAGGTGGTACAAGTATGCATCTGTGGGTTTGACCATGTTTGGTGTTTATTTGGTTGCTAAGCATTCTATCCAATACATCATTGAGAGAAAGCGCCTTTGGGAATTGCAGAAAAGGGTTGTTGCTGCAGCTGCTAAAAGATCTGGGGAAGATAGTGAAGGTTCAAACGAAAAAGATGATAGTGCATCAGATGGAAACAAGAGAGACCGTATGATGCCCAATTTATGTGTGATATGCCTTGAACAGGATTATAATGCGGTTTTTGTCCCGTGTGGTCATATGTGCTGTTGTACAACTTGCTCCTTGCACCTGACCACCTGTCCTCTCTGCCGGCGACGAATTGATCAGGCTGTAAAGACTTTCCGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GIDE zf-C3HC4_3

Protein Analysis

343

Amino Acids

37.87

Weight (kDa)

8.77

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIDE PF12483 85 - 234 1.3e-39 E3 Ubiquitin ligase
zf-C3HC4_3 PF13920 293 - 335 6e-14 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 295 - 336 2.5e-07 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011309)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63900
fragaria_vesca FvH4_2g24080 FvH4_2g24080
malus_domestica MD03G1270200.v1.1 MD11G1291200.v1.1
prunus_persica Prupe.8G246900_v2.0.a1
pyrus_communis pycom03g21540 pycom11g25810
rosa_chinensis RchiOBHm_Chr6g0291861
rosa_laevigata RLG00000012082
rosa_multiflora Rmu_sc0001043.1_g000019 Rmu_sc0002484.1_g000001
rosa_roxburghii Rroxscaffold_7G00175690
rosa_rugosa Rorug06G0221700
rosa_samantha Rh6AG334800 Rh6BG342300 Rh6CG348100 Rh6DG335100
rosa_wichuraiana Rw6G028920 Rw6G029140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 909
Acc65I GGTACC 1 cut(s) 569
AccB1I GGYRCC 1 cut(s) 569
AciI CCGC 3 cut(s) 96, 914, 1021
AclWI GGATC 1 cut(s) 435
AcsI RAATTY 1 cut(s) 146
AfaI GTAC 7 cut(s) 341, 450, 513, 526, 571, 668, 949
AfiI CCNNNNNNNGG 4 cut(s) 14, 102, 596, 901
AgeI ACCGGT 1 cut(s) 521
AgsI TTSAA 6 cut(s) 268, 318, 428, 584, 822, 899
AhdI GACNNNNNGTC 1 cut(s) 975
AjnI CCWGG 1 cut(s) 335
AjuI GAANNNNNNNTTGG 4 cut(s) 511, 543, 739, 771
AluBI AGCT 5 cut(s) 41, 189, 386, 634, 788
AluI AGCT 5 cut(s) 41, 189, 386, 634, 788
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 2 cut(s) 616, 855
AlwI GGATC 1 cut(s) 435
AoxI GGCC 2 cut(s) 475, 599
ApeKI GCWGC 5 cut(s) 41, 386, 782, 785, 788
ApoI RAATTY 1 cut(s) 146
AseI ATTAAT 1 cut(s) 108
AsiGI ACCGGT 1 cut(s) 521
Asp718I GGTACC 1 cut(s) 569
AspLEI GCGC 1 cut(s) 753
AspS9I GGNCC 1 cut(s) 599
AsuHPI GGTGA 1 cut(s) 557
BaeI ACNNNNGTAYC 2 cut(s) 553, 586
BanI GGYRCC 1 cut(s) 569
BauI CACGAG 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 5 cut(s) 28, 373, 769, 775, 797
BccI CCATC 3 cut(s) 209, 344, 842
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 337
BciVI GTATCC 1 cut(s) 285
BclI TGATCA 2 cut(s) 628, 1000
BcoDI GTCTC 2 cut(s) 616, 855
BfmI CTRYAG 1 cut(s) 783
BfoI RGCGCY 1 cut(s) 754
BfuI GTATCC 1 cut(s) 285
BglII AGATCT 1 cut(s) 797
BisI GCNGC 5 cut(s) 42, 387, 783, 786, 789
BlpI GCTNAGC 1 cut(s) 717
BlsI GCNGC 5 cut(s) 43, 388, 784, 787, 790
Bme1390I CCNGG 1 cut(s) 337
BmeRI GACNNNNNGTC 1 cut(s) 975
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 2 cut(s) 571, 600
BmrFI CCNGG 1 cut(s) 337
BmsI GCATC 7 cut(s) 67, 268, 300, 474, 686, 851, 861
Bpu1102I GCTNAGC 1 cut(s) 717
BpuEI CTTGAG 1 cut(s) 464
BsaAI YACGTR 1 cut(s) 452
BsaI GGTCTC 1 cut(s) 855
BsaJI CCNNGG 4 cut(s) 8, 335, 472, 646
BsaWI WCCGGW 1 cut(s) 521
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 102, 596, 901
Bse118I RCCGGY 3 cut(s) 406, 521, 987
Bse1I ACTGG 3 cut(s) 158, 223, 361
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 4 cut(s) 8, 335, 472, 646
BseGI GGATG 2 cut(s) 82, 352
BseLI CCNNNNNNNGG 4 cut(s) 14, 102, 596, 901
BseMII CTCAG 2 cut(s) 23, 605
BseNI ACTGG 3 cut(s) 158, 223, 361
BseXI GCAGC 5 cut(s) 28, 373, 769, 775, 797
BshFI GGCC 2 cut(s) 477, 601
BshNI GGYRCC 1 cut(s) 569
BshTI ACCGGT 1 cut(s) 521
BsiHKAI GWGCWC 1 cut(s) 382
BsiSI CCGG 3 cut(s) 407, 522, 988
BsiWI CGTACG 1 cut(s) 448
BslFI GGGAC 2 cut(s) 469, 908
BslI CCNNNNNNNGG 4 cut(s) 14, 102, 596, 901
BsmAI GTCTC 2 cut(s) 616, 855
BsmFI GGGAC 2 cut(s) 469, 908
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 2 cut(s) 477, 601
Bso31I GGTCTC 1 cut(s) 855
Bsp1286I GDGCHC 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 947
Bsp143I GATC 4 cut(s) 440, 628, 797, 1000
Bsp1720I GCTNAGC 1 cut(s) 717
BspACI CCGC 3 cut(s) 96, 914, 1021
BspANI GGCC 2 cut(s) 477, 601
BspCNI CTCAG 2 cut(s) 24, 604
BspLI GGNNCC 2 cut(s) 571, 600
BspMAI CTGCAG 1 cut(s) 787
BspPI GGATC 1 cut(s) 435
BspT107I GGYRCC 1 cut(s) 569
BspTNI GGTCTC 1 cut(s) 855
BsrFI RCCGGY 3 cut(s) 406, 521, 987
BsrGI TGTACA 1 cut(s) 947
BsrI ACTGG 3 cut(s) 158, 223, 361
BssAI RCCGGY 3 cut(s) 406, 521, 987
BssECI CCNNGG 4 cut(s) 8, 335, 472, 646
BssMI GATC 4 cut(s) 440, 628, 797, 1000
BssSI CACGAG 1 cut(s) 381
BssT1I CCWWGG 3 cut(s) 8, 472, 646
Bst2BI CACGAG 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 2 cut(s) 209, 866
Bst6I CTCTTC 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 386
BstAUI TGTACA 1 cut(s) 947
BstBAI YACGTR 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 283, 989
BstDEI CTNAG 3 cut(s) 32, 591, 717
BstENI CCTNNNNNAGG 1 cut(s) 899
BstF5I GGATG 2 cut(s) 82, 352
BstH2I RGCGCY 1 cut(s) 754
BstHHI GCGC 1 cut(s) 753
BstKTI GATC 4 cut(s) 443, 631, 800, 1003
BstMAI GTCTC 2 cut(s) 616, 855
BstMBI GATC 4 cut(s) 440, 628, 797, 1000
BstMWI GCNNNNNNNGC 2 cut(s) 386, 788
BstNI CCWGG 1 cut(s) 337
BstSCI CCNGG 1 cut(s) 335
BstSFI CTRYAG 1 cut(s) 783
BstV1I GCAGC 5 cut(s) 28, 373, 769, 775, 797
BstX2I RGATCY 1 cut(s) 797
BstYI RGATCY 1 cut(s) 797
BsuI GTATCC 1 cut(s) 285
BsuRI GGCC 2 cut(s) 477, 601
BtsCI GGATG 2 cut(s) 82, 352
BtsI GCAGTG 1 cut(s) 73
BtsIMutI CAGTG 3 cut(s) 73, 165, 216
Cac8I GCNNGC 2 cut(s) 283, 989
CfoI GCGC 1 cut(s) 753
Cfr10I RCCGGY 3 cut(s) 406, 521, 987
Cfr13I GGNCC 1 cut(s) 599
Csp6I GTAC 7 cut(s) 340, 449, 512, 525, 570, 667, 948
CspAI ACCGGT 1 cut(s) 521
CviAII CATG 2 cut(s) 288, 694
CviQI GTAC 7 cut(s) 340, 449, 512, 525, 570, 667, 948
DdeI CTNAG 3 cut(s) 32, 591, 717
DpnI GATC 4 cut(s) 442, 630, 799, 1002
DpnII GATC 4 cut(s) 440, 628, 797, 1000
DriI GACNNNNNGTC 1 cut(s) 975
Eam1104I CTCTTC 1 cut(s) 423
Eam1105I GACNNNNNGTC 1 cut(s) 975
EarI CTCTTC 1 cut(s) 423
EciI GGCGGA 2 cut(s) 85, 1010
Eco130I CCWWGG 3 cut(s) 8, 472, 646
Eco147I AGGCCT 1 cut(s) 477
Eco31I GGTCTC 1 cut(s) 855
EcoNI CCTNNNNNAGG 1 cut(s) 899
EcoRII CCWGG 1 cut(s) 335
EcoT14I CCWWGG 3 cut(s) 8, 472, 646
EcoT22I ATGCAT 1 cut(s) 679
ErhI CCWWGG 3 cut(s) 8, 472, 646
FaeI CATG 2 cut(s) 291, 697
FaqI GGGAC 2 cut(s) 469, 908
FatI CATG 2 cut(s) 287, 693
FauNDI CATATG 1 cut(s) 936
FbaI TGATCA 2 cut(s) 628, 1000
Fnu4HI GCNGC 5 cut(s) 42, 387, 783, 786, 789
FokI GGATG 2 cut(s) 89, 359
Fsp4HI GCNGC 5 cut(s) 42, 387, 783, 786, 789
GlaI GCGC 1 cut(s) 752
GluI GCNGC 5 cut(s) 42, 387, 783, 786, 789
HaeII RGCGCY 1 cut(s) 754
HaeIII GGCC 2 cut(s) 477, 601
HapII CCGG 3 cut(s) 407, 522, 988
HhaI GCGC 1 cut(s) 753
Hin1II CATG 2 cut(s) 291, 697
Hin6I GCGC 1 cut(s) 751
HinP1I GCGC 1 cut(s) 751
HinfI GANTC 6 cut(s) 85, 140, 299, 431, 492, 580
HpaII CCGG 3 cut(s) 407, 522, 988
HphI GGTGA 1 cut(s) 557
Hpy166II GTNNAC 2 cut(s) 216, 224
Hpy188I TCNGA 7 cut(s) 33, 105, 145, 193, 579, 594, 847
Hpy188III TCNNGA 2 cut(s) 435, 481
Hpy8I GTNNAC 2 cut(s) 216, 224
Hpy99I CGWCG 1 cut(s) 996
HpyAV CCTTC 3 cut(s) 62, 589, 809
HpyCH4III ACNGT 2 cut(s) 209, 866
HpyCH4IV ACGT 1 cut(s) 451
HpyCH4V TGCA 8 cut(s) 80, 170, 413, 677, 766, 785, 842, 964
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 788
HpyF3I CTNAG 3 cut(s) 32, 591, 717
HpySE526I ACGT 1 cut(s) 451
Hsp92II CATG 2 cut(s) 291, 697
HspAI GCGC 1 cut(s) 751
KpnI GGTACC 1 cut(s) 573
KroI GCCGGC 1 cut(s) 987
KroNI GCCGGC 1 cut(s) 989
Ksp22I TGATCA 2 cut(s) 628, 1000
Kzo9I GATC 4 cut(s) 440, 628, 797, 1000
LmnI GCTCC 4 cut(s) 38, 63, 377, 962
Lsp1109I GCAGC 5 cut(s) 28, 373, 769, 775, 797
LweI GCATC 7 cut(s) 67, 268, 300, 474, 686, 851, 861
MaeII ACGT 1 cut(s) 451
MalI GATC 4 cut(s) 442, 630, 799, 1002
MboI GATC 4 cut(s) 440, 628, 797, 1000
MboII GAAGA 2 cut(s) 440, 818
MflI RGATCY 1 cut(s) 797
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 7 cut(s) 122, 131, 146, 501, 761, 877, 996
MlyI GAGTC 2 cut(s) 440, 501
MmeI TCCRAC 2 cut(s) 117, 542
MnlI CCTC 9 cut(s) 221, 235, 412, 455, 488, 501, 541, 600, 990
Mph1103I ATGCAT 1 cut(s) 679
MroNI GCCGGC 1 cut(s) 987
MseI TTAA 4 cut(s) 90, 108, 116, 1027
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 788
MspI CCGG 3 cut(s) 407, 522, 988
MspR9I CCNGG 1 cut(s) 337
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 2 cut(s) 386, 788
NaeI GCCGGC 1 cut(s) 989
NdeI CATATG 1 cut(s) 936
NdeII GATC 4 cut(s) 440, 628, 797, 1000
NgoMIV GCCGGC 1 cut(s) 987
NlaIII CATG 2 cut(s) 291, 697
NlaIV GGNNCC 2 cut(s) 571, 600
NsiI ATGCAT 1 cut(s) 679
PceI AGGCCT 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 583
PctI GAATGC 1 cut(s) 721
PdiI GCCGGC 1 cut(s) 989
PfeI GAWTC 4 cut(s) 85, 140, 299, 580
Pfl23II CGTACG 1 cut(s) 448
PinAI ACCGGT 1 cut(s) 521
PkrI GCNGC 5 cut(s) 43, 388, 784, 787, 790
PleI GAGTC 2 cut(s) 439, 500
PpsI GAGTC 2 cut(s) 439, 500
Ppu21I YACGTR 1 cut(s) 452
PshBI ATTAAT 1 cut(s) 108
PsiI TTATAA 1 cut(s) 909
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspLI CGTACG 1 cut(s) 448
PspN4I GGNNCC 2 cut(s) 571, 600
PspPI GGNCC 1 cut(s) 599
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 797
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 7 cut(s) 341, 450, 513, 526, 571, 668, 949
RsaNI GTAC 7 cut(s) 340, 449, 512, 525, 570, 667, 948
RseI CAYNNNNRTG 1 cut(s) 276
SaqAI TTAA 4 cut(s) 90, 108, 116, 1027
SatI GCNGC 5 cut(s) 42, 387, 783, 786, 789
Sau3AI GATC 4 cut(s) 440, 628, 797, 1000
Sau96I GGNCC 1 cut(s) 599
SchI GAGTC 2 cut(s) 440, 501
ScrFI CCNGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 382
SfaNI GCATC 7 cut(s) 67, 268, 300, 474, 686, 851, 861
SfcI CTRYAG 1 cut(s) 783
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 7 cut(s) 122, 131, 146, 501, 761, 877, 996
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 3 cut(s) 96, 914, 1021
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 335
StyI CCWWGG 3 cut(s) 8, 472, 646
TaaI ACNGT 2 cut(s) 209, 866
TaiI ACGT 1 cut(s) 454
TasI AATT 7 cut(s) 122, 131, 146, 501, 761, 877, 996
TatI WGTACW 1 cut(s) 947
TfiI GAWTC 4 cut(s) 85, 140, 299, 580
Tru1I TTAA 4 cut(s) 90, 108, 116, 1027
Tru9I TTAA 4 cut(s) 90, 108, 116, 1027
TscAI CASTG 3 cut(s) 73, 165, 223
TseI GCWGC 5 cut(s) 41, 386, 782, 785, 788
TspRI CASTG 3 cut(s) 73, 165, 223
VspI ATTAAT 1 cut(s) 108
XagI CCTNNNNNAGG 1 cut(s) 899
XapI RAATTY 1 cut(s) 146
Zsp2I ATGCAT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.