pycom12401g00210

Proton-conducting membrane transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012401
OG0015226
Physical Location & Seq
Reverse (-)
103518 .. 103922
405 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 405 bp
ATGTTAAGTCATGGACTGGTTCCTTCAGCCCTTTTTCTATGTGTTGGTGTTCTATATGACCGACATAAGACTCGACTTGTTAGATATTACGGAGGTTCAGTGAGCACCATGCCGAATCTCTCTACCATTTCCTTCTCTTCCACTTTGGCCAATATGAGTTCACCCGGTACTAGCAGCTTTATCGGGGAATTTCCCATCTTAGTAGGAGCTTTCCAAAGAAATAGCTTAGTAGCCACATTAGCAGCGCTTGGGATGATTTTAGGCGCGGCGTATTCCCTTTGGCTATATAATCGTTTGGTTTCTGGAAATTTAAAAGCCGATTTCCTACATAAATTCTCCGATCCAAATGGCAGAGAAGTTTCTATATTTATACCCTTTCTTGTTGGAGGGGCGACCGTCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Proton_antipo_M

Protein Analysis

135

Amino Acids

14.42

Weight (kDa)

9.8

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 1 - 82 2.4e-13 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 266
AciI CCGC 1 cut(s) 266
AclWI GGATC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 3 cut(s) 188, 307, 332
AcuI CTGAAG 1 cut(s) 9
AfaI GTAC 1 cut(s) 169
AfeI AGCGCT 1 cut(s) 246
AluBI AGCT 3 cut(s) 177, 209, 225
AluI AGCT 3 cut(s) 177, 209, 225
Alw21I GWGCWC 1 cut(s) 107
AlwI GGATC 1 cut(s) 335
Aor51HI AGCGCT 1 cut(s) 246
AoxI GGCC 1 cut(s) 147
ApeKI GCWGC 2 cut(s) 174, 242
ApoI RAATTY 3 cut(s) 188, 307, 332
AspLEI GCGC 2 cut(s) 247, 266
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 1 cut(s) 153
BalI TGGCCA 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 2 cut(s) 186, 254
BccI CCATC 1 cut(s) 203
BcgI CGANNNNNNTGC 2 cut(s) 163, 197
BcnI CCSGG 1 cut(s) 165
BfaI CTAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 248
BisI GCNGC 3 cut(s) 175, 243, 267
BlsI GCNGC 3 cut(s) 176, 244, 268
Bme1390I CCNGG 1 cut(s) 165
BmiI GGNNCC 1 cut(s) 21
BmrFI CCNGG 1 cut(s) 165
BpuMI CCSGG 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bse1I ACTGG 1 cut(s) 21
BseGI GGATG 1 cut(s) 258
BseNI ACTGG 1 cut(s) 21
BseXI GCAGC 2 cut(s) 186, 254
Bsh1236I CGCG 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 396
BshFI GGCC 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 107
BsiSI CCGG 1 cut(s) 165
BsnI GGCC 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 107
Bsp143I GATC 1 cut(s) 340
BspACI CCGC 1 cut(s) 266
BspANI GGCC 1 cut(s) 149
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 21
BspPI GGATC 1 cut(s) 335
BsrI ACTGG 1 cut(s) 21
BssMI GATC 1 cut(s) 340
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 1 cut(s) 142
BstDEI CTNAG 2 cut(s) 199, 226
BstF5I GGATG 1 cut(s) 258
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 2 cut(s) 247, 266
BstKTI GATC 1 cut(s) 343
BstMBI GATC 1 cut(s) 340
BstMCI CGRYCG 1 cut(s) 396
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 163
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 2 cut(s) 186, 254
BsuRI GGCC 1 cut(s) 149
BtsCI GGATG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 105
CfoI GCGC 2 cut(s) 247, 266
Csp6I GTAC 1 cut(s) 168
CviAII CATG 2 cut(s) 11, 109
CviJI RGCY 8 cut(s) 29, 149, 177, 209, 225, 233, 283, 317
CviKI_1 RGCY 8 cut(s) 29, 149, 177, 209, 225, 233, 283, 317
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 2 cut(s) 199, 226
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
DraI TTTAAA 1 cut(s) 312
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
Eco47III AGCGCT 1 cut(s) 246
Eco57I CTGAAG 1 cut(s) 9
FaeI CATG 2 cut(s) 14, 112
FatI CATG 2 cut(s) 10, 108
Fnu4HI GCNGC 3 cut(s) 175, 243, 267
FokI GGATG 1 cut(s) 265
Fsp4HI GCNGC 3 cut(s) 175, 243, 267
FspBI CTAG 1 cut(s) 171
GlaI GCGC 2 cut(s) 246, 265
GluI GCNGC 3 cut(s) 175, 243, 267
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 1 cut(s) 149
HapII CCGG 1 cut(s) 165
HhaI GCGC 2 cut(s) 247, 266
Hin1II CATG 2 cut(s) 14, 112
Hin6I GCGC 2 cut(s) 245, 264
HinP1I GCGC 2 cut(s) 245, 264
HinfI GANTC 2 cut(s) 70, 115
HpaII CCGG 1 cut(s) 165
HphI GGTGA 1 cut(s) 153
Hpy166II GTNNAC 1 cut(s) 161
Hpy188I TCNGA 1 cut(s) 340
Hpy188III TCNNGA 1 cut(s) 303
Hpy8I GTNNAC 1 cut(s) 161
HpyAV CCTTC 2 cut(s) 33, 142
HpyCH4III ACNGT 1 cut(s) 397
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 2 cut(s) 199, 226
Hsp92II CATG 2 cut(s) 14, 112
HspAI GCGC 2 cut(s) 245, 264
Kzo9I GATC 1 cut(s) 340
LmnI GCTCC 1 cut(s) 206
LpnPI CCDG 3 cut(s) 2, 178, 288
Lsp1109I GCAGC 2 cut(s) 186, 254
MaeI CTAG 1 cut(s) 171
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 1 cut(s) 129
MhlI GDGCHC 1 cut(s) 107
MlsI TGGCCA 1 cut(s) 149
MluCI AATT 3 cut(s) 188, 307, 332
MluNI TGGCCA 1 cut(s) 149
MlyI GAGTC 1 cut(s) 64
MmeI TCCRAC 1 cut(s) 364
MnlI CCTC 2 cut(s) 86, 380
Mox20I TGGCCA 1 cut(s) 149
MscI TGGCCA 1 cut(s) 149
MseI TTAA 2 cut(s) 5, 311
Msp20I TGGCCA 1 cut(s) 149
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 1 cut(s) 165
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 1 cut(s) 239
NciI CCSGG 1 cut(s) 165
NdeII GATC 1 cut(s) 340
NlaIII CATG 2 cut(s) 14, 112
NlaIV GGNNCC 1 cut(s) 21
PfeI GAWTC 1 cut(s) 115
PkrI GCNGC 3 cut(s) 176, 244, 268
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
PspN4I GGNNCC 1 cut(s) 21
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
SaqAI TTAA 2 cut(s) 5, 311
SatI GCNGC 3 cut(s) 175, 243, 267
Sau3AI GATC 1 cut(s) 340
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 1 cut(s) 107
SetI ASST 4 cut(s) 97, 179, 211, 227
Sse9I AATT 3 cut(s) 188, 307, 332
SsiI CCGC 1 cut(s) 266
SspMI CTAG 1 cut(s) 171
StyD4I CCNGG 1 cut(s) 163
TaaI ACNGT 1 cut(s) 397
TaqI TCGA 1 cut(s) 73
TaqII GACCGA 1 cut(s) 75
TasI AATT 3 cut(s) 188, 307, 332
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 1 cut(s) 115
Tru1I TTAA 2 cut(s) 5, 311
Tru9I TTAA 2 cut(s) 5, 311
TscAI CASTG 1 cut(s) 105
TseI GCWGC 2 cut(s) 174, 242
TspGWI ACGGA 2 cut(s) 105, 389
TspRI CASTG 1 cut(s) 105
XapI RAATTY 3 cut(s) 188, 307, 332
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.