RLG00000000443

Proton-conducting membrane transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015226
Physical Location & Seq
Forward (+)
991801 .. 992831
1031 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000443

Sequence Viewer

Length: 744 bp
ATGGTCTTTCTCAACGTGGTGTATAGCACGAAAAACCTTTCGATACAAGAAAGGGCCGTTCACATGAAAGAAGAGAATCACTCCTTTCTTTCTTCTCTTCTTTCTCTTTCCCGAGGGGGAAAGAGAAATAGGGTCTTATGGAGGGAGGGGGGGAAAGGATTGGGCCTACCTATCCCGATAGGACCTCATAAAGGAACGGGAGCTGTTGAGAGGTTCCATATTGCCGAGCCGAAGGGCAGCACTTCTATACGCGATCGTAGTATGTCACGTCGTCTCGTCCTCGCTGCATTGAAGAGTACCTATGCACTATTTCCGGGAATTGGAGGTAGCATTCTACCGATGTTAAGTCATGGACTGGTTCCTTCAGCCCTTTTTCTATGTGTTGGTGTTCTATATGACCGACATAAGACTCGACTTGTTAGATATTACGGAGGTTCAGTGAGCACCATGCCGAATCTCTCTACCATTTCCTTCTCTTCCACTTTGGCCAATATGAGTTCACCTGGTACTAGCAGCTTTATCGGGGAATTTCCCATCTTAGTAGGAGCTTTCCAAAGAAATAGCTTAGTAGCCACATTAGCTGCGCTTGGGATGATTTTAGGCGCGGCGTATTCCCTTTGGCTATATAATCGTTTGGTTTCTGGAAATTTAAAACCCGATTTCCTCCATAAATTCTCCGATCCAAATGGCAGAGAAGTTTCCATATTTATACCTTTTCTTGTTGGAGGGGCGACCGTCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Proton_antipo_M

Protein Analysis

248

Amino Acids

26.78

Weight (kDa)

10.45

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 105 - 195 3.6e-14 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 252, 605
AciI CCGC 1 cut(s) 605
AclWI GGATC 1 cut(s) 674
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 3 cut(s) 527, 646, 671
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 2 cut(s) 298, 508
AfiI CCNNNNNNNGG 2 cut(s) 191, 320
AgsI TTSAA 1 cut(s) 292
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 1 cut(s) 502
AluBI AGCT 5 cut(s) 203, 516, 548, 564, 581
AluI AGCT 5 cut(s) 203, 516, 548, 564, 581
Alw21I GWGCWC 1 cut(s) 446
Alw26I GTCTC 1 cut(s) 278
AlwI GGATC 1 cut(s) 674
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 3 cut(s) 54, 163, 486
ApeKI GCWGC 4 cut(s) 237, 284, 513, 581
ApoI RAATTY 3 cut(s) 527, 646, 671
AspLEI GCGC 2 cut(s) 586, 605
AspS9I GGNCC 3 cut(s) 54, 163, 182
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 1 cut(s) 492
AvaI CYCGRG 1 cut(s) 111
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 488
Bbv12I GWGCWC 1 cut(s) 446
BbvI GCAGC 4 cut(s) 249, 271, 525, 568
BccI CCATC 1 cut(s) 542
BceAI ACGGC 1 cut(s) 41
BcgI CGANNNNNNTGC 2 cut(s) 502, 536
BciT130I CCWGG 1 cut(s) 504
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 278
BfaI CTAG 1 cut(s) 510
BisI GCNGC 5 cut(s) 238, 285, 514, 582, 606
BlsI GCNGC 5 cut(s) 239, 286, 515, 583, 607
Bme1390I CCNGG 2 cut(s) 315, 504
Bme18I GGWCC 1 cut(s) 182
BmeT110I CYCGRG 1 cut(s) 111
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 3 cut(s) 54, 163, 182
BmiI GGNNCC 2 cut(s) 215, 360
BmrFI CCNGG 2 cut(s) 315, 504
BplI GAGNNNNNCTC 2 cut(s) 65, 97
BpuMI CCSGG 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 112
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 320
Bse1I ACTGG 1 cut(s) 360
BseBI CCWGG 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 1 cut(s) 597
BseLI CCNNNNNNNGG 2 cut(s) 191, 320
BseNI ACTGG 1 cut(s) 360
BseXI GCAGC 4 cut(s) 249, 271, 525, 568
Bsh1236I CGCG 2 cut(s) 252, 605
Bsh1285I CGRYCG 2 cut(s) 256, 735
BshFI GGCC 3 cut(s) 56, 165, 488
BsiEI CGRYCG 2 cut(s) 256, 735
BsiHKAI GWGCWC 1 cut(s) 446
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 1 cut(s) 314
BslI CCNNNNNNNGG 2 cut(s) 191, 320
BsmAI GTCTC 1 cut(s) 278
BsmBI CGTCTC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 330
BsnI GGCC 3 cut(s) 56, 165, 488
BsoBI CYCGRG 1 cut(s) 111
Bsp1286I GDGCHC 1 cut(s) 446
Bsp143I GATC 2 cut(s) 253, 679
BspACI CCGC 1 cut(s) 605
BspANI GGCC 3 cut(s) 56, 165, 488
BspFNI CGCG 2 cut(s) 252, 605
BspLI GGNNCC 2 cut(s) 215, 360
BspPI GGATC 1 cut(s) 674
BsrI ACTGG 1 cut(s) 360
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 2 cut(s) 253, 679
Bst2UI CCWGG 1 cut(s) 504
Bst4CI ACNGT 1 cut(s) 736
Bst6I CTCTTC 4 cut(s) 66, 102, 287, 481
BstDEI CTNAG 2 cut(s) 538, 565
BstENI CCTNNNNNAGG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 597
BstFNI CGCG 2 cut(s) 252, 605
BstHHI GCGC 2 cut(s) 586, 605
BstKTI GATC 2 cut(s) 256, 682
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 2 cut(s) 253, 679
BstMCI CGRYCG 2 cut(s) 256, 735
BstMWI GCNNNNNNNGC 1 cut(s) 578
BstNI CCWGG 1 cut(s) 504
BstSCI CCNGG 2 cut(s) 313, 502
BstUI CGCG 2 cut(s) 252, 605
BstV1I GCAGC 4 cut(s) 249, 271, 525, 568
BsuRI GGCC 3 cut(s) 56, 165, 488
BtrI CACGTC 1 cut(s) 269
BtsCI GGATG 1 cut(s) 597
BtsIMutI CAGTG 1 cut(s) 444
CfoI GCGC 2 cut(s) 586, 605
Cfr13I GGNCC 3 cut(s) 54, 163, 182
CsiI ACCWGGT 1 cut(s) 502
Csp6I GTAC 2 cut(s) 297, 507
CviAII CATG 3 cut(s) 64, 350, 448
CviQI GTAC 2 cut(s) 297, 507
DdeI CTNAG 2 cut(s) 538, 565
DpnI GATC 2 cut(s) 255, 681
DpnII GATC 2 cut(s) 253, 679
DraI TTTAAA 1 cut(s) 651
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 4 cut(s) 66, 102, 287, 481
EarI CTCTTC 4 cut(s) 66, 102, 287, 481
Eco47I GGWCC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 348
Eco88I CYCGRG 1 cut(s) 111
EcoNI CCTNNNNNAGG 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 502
Esp3I CGTCTC 1 cut(s) 278
FaeI CATG 3 cut(s) 67, 353, 451
FatI CATG 3 cut(s) 63, 349, 447
Fnu4HI GCNGC 5 cut(s) 238, 285, 514, 582, 606
FokI GGATG 1 cut(s) 604
Fsp4HI GCNGC 5 cut(s) 238, 285, 514, 582, 606
FspBI CTAG 1 cut(s) 510
GlaI GCGC 2 cut(s) 585, 604
GluI GCNGC 5 cut(s) 238, 285, 514, 582, 606
HaeIII GGCC 3 cut(s) 56, 165, 488
HapII CCGG 1 cut(s) 314
HhaI GCGC 2 cut(s) 586, 605
Hin1II CATG 3 cut(s) 67, 353, 451
Hin6I GCGC 2 cut(s) 584, 603
HinP1I GCGC 2 cut(s) 584, 603
HinfI GANTC 3 cut(s) 76, 409, 454
HpaII CCGG 1 cut(s) 314
HphI GGTGA 1 cut(s) 492
Hpy166II GTNNAC 2 cut(s) 61, 500
Hpy188I TCNGA 1 cut(s) 679
Hpy188III TCNNGA 3 cut(s) 111, 175, 642
Hpy8I GTNNAC 2 cut(s) 61, 500
Hpy99I CGWCG 1 cut(s) 273
HpyAV CCTTC 3 cut(s) 226, 372, 481
HpyCH4III ACNGT 1 cut(s) 736
HpyCH4IV ACGT 2 cut(s) 15, 268
HpyCH4V TGCA 2 cut(s) 287, 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 578
HpyF3I CTNAG 2 cut(s) 538, 565
HpySE526I ACGT 2 cut(s) 15, 268
Hsp92II CATG 3 cut(s) 67, 353, 451
HspAI GCGC 2 cut(s) 584, 603
Kzo9I GATC 2 cut(s) 253, 679
LmnI GCTCC 2 cut(s) 200, 545
LpnPI CCDG 5 cut(s) 327, 341, 489, 516, 627
Lsp1109I GCAGC 4 cut(s) 249, 271, 525, 568
MabI ACCWGGT 1 cut(s) 502
MaeI CTAG 1 cut(s) 510
MaeII ACGT 2 cut(s) 15, 268
MaeIII GTNAC 1 cut(s) 264
MalI GATC 2 cut(s) 255, 681
MboI GATC 2 cut(s) 253, 679
MboII GAAGA 5 cut(s) 83, 84, 89, 304, 468
MhlI GDGCHC 1 cut(s) 446
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 4 cut(s) 318, 527, 646, 671
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 1 cut(s) 403
MmeI TCCRAC 1 cut(s) 703
Mox20I TGGCCA 1 cut(s) 488
MscI TGGCCA 1 cut(s) 488
MseI TTAA 2 cut(s) 344, 650
Msp20I TGGCCA 1 cut(s) 488
MspI CCGG 1 cut(s) 314
MspR9I CCNGG 2 cut(s) 315, 504
Mva1269I GAATGC 1 cut(s) 330
MvaI CCWGG 1 cut(s) 504
MvnI CGCG 2 cut(s) 252, 605
MwoI GCNNNNNNNGC 1 cut(s) 578
NciI CCSGG 1 cut(s) 315
NdeII GATC 2 cut(s) 253, 679
NlaIII CATG 3 cut(s) 67, 353, 451
NlaIV GGNNCC 2 cut(s) 215, 360
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 264
PctI GAATGC 1 cut(s) 330
PfeI GAWTC 2 cut(s) 76, 454
PfoI TCCNGGA 1 cut(s) 313
PkrI GCNGC 5 cut(s) 239, 286, 515, 583, 607
Ple19I CGATCG 1 cut(s) 256
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PpuMI RGGWCCY 1 cut(s) 182
Psp5II RGGWCCY 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 502
PspGI CCWGG 1 cut(s) 502
PspN4I GGNNCC 2 cut(s) 215, 360
PspPI GGNCC 3 cut(s) 54, 163, 182
PspPPI RGGWCCY 1 cut(s) 182
PvuI CGATCG 1 cut(s) 256
RsaI GTAC 2 cut(s) 298, 508
RsaNI GTAC 2 cut(s) 297, 507
SaqAI TTAA 2 cut(s) 344, 650
SatI GCNGC 5 cut(s) 238, 285, 514, 582, 606
Sau3AI GATC 2 cut(s) 253, 679
Sau96I GGNCC 3 cut(s) 54, 163, 182
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 2 cut(s) 315, 504
SduI GDGCHC 1 cut(s) 446
SexAI ACCWGGT 1 cut(s) 502
SinI GGWCC 1 cut(s) 182
Sse9I AATT 4 cut(s) 318, 527, 646, 671
SsiI CCGC 1 cut(s) 605
SspMI CTAG 1 cut(s) 510
StyD4I CCNGG 2 cut(s) 313, 502
TaaI ACNGT 1 cut(s) 736
TaiI ACGT 2 cut(s) 18, 271
TaqI TCGA 2 cut(s) 41, 412
TaqII GACCGA 1 cut(s) 414
TasI AATT 4 cut(s) 318, 527, 646, 671
TauI GCSGC 1 cut(s) 608
TfiI GAWTC 2 cut(s) 76, 454
Tru1I TTAA 2 cut(s) 344, 650
Tru9I TTAA 2 cut(s) 344, 650
TscAI CASTG 1 cut(s) 444
TseFI GTSAC 1 cut(s) 264
TseI GCWGC 4 cut(s) 237, 284, 513, 581
Tsp45I GTSAC 1 cut(s) 264
TspDTI ATGAA 1 cut(s) 80
TspGWI ACGGA 2 cut(s) 444, 728
TspRI CASTG 1 cut(s) 444
VpaK11BI GGWCC 1 cut(s) 182
XagI CCTNNNNNAGG 1 cut(s) 189
XapI RAATTY 3 cut(s) 527, 646, 671
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.