RCHIOBHM_MTG0498651

Proton-conducting membrane transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0015226
Physical Location & Seq
Reverse (-)
136887 .. 138397
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15629

Sequence Viewer

Length: 1110 bp
ATGGTCTTTCTCAACGTGGTGTATAGCACGAAAAACCTTTCGATACAAGAAAGGGCCGTTCACATGAAAGAAGAGAATCACTCCTTTCTTTCTTCTCTTCTTTCTCTTTCCCGAGGGGGAAAGAGAAATAGGGTCTTATGGAGGGAGGGGGGGAAAGGATTGGGCCTACCTATCCCGATAGGACCTCATAAAGGAACGGGAGCTGTTGAGAGGTTCCATATTGCCGAGCCGAAGGGCAGCACTTCTATACGCGATCGTAGTATGTCACGTCGTCTCGGAATTGGAGGTAGCATTCTACCGATGTTAAGTCATGGACTGGTTCCTTCAGCCCTTTTTCTATGTGTTGGTGTTCTATATGACCGACATAAGACTCGACTTGTTAGATATTACGGAGGTTCAGTGAGCACCATGCCGAATCTCTCTACCATTTCCTTCTCTTCCACTTTGGCCAATATGAGTTCACCTGGTACTAGCAGCTTTATCGGGGAATTTCCCATCTTAGTAGGAGCTTTCCAAAGAAATAGCTTAGTAGCCACATTAGCTGCGCTTGGGATGATTTTAGGCGCGGCGTATTCCCTTTGGCTATATAATCGTTTGGTTTCTGGAAATTTAAAACCCGATTTCCTCCATAAATTCTCCGATCCAAATGGCAGAGAAGTTTCCATATTTATACCTTTTCTTGTTGGAGGGGCGACCGTGCTTGCGATGGGACGGATGCGACTTCCCTCAGTTGGTTTGGGTGGCATAGCCCGTTGCAGAAGTCCCCCCTTTTTTTTGATCCATTTCTTTAGTTTAGTCTTTAGGGAGCCAAAGCTTGACTTTACTAATAAAATAAGGCTCGCGCAGGGGCGCTCACGTTTTTTGGCTGAGCCGTATCTTGGGACCGAGATAAAGAAAGGACGGGGGGCAACCATGCATGGTACTTCTCGCCCGTGTCTCCGAGGGACAGTTGAACGAGCGATTCATGAATGCTGCCGGGTTGGACGAGCCAATAACTCGAACGCGTTCGGTCTGTTTTTTGAGCAAGAATCACAGCGTTACCTTACCTTCACCATGATACGGACTCCAAGTTCTTATGGCAGAGCACGAGGAGATTTATCATCAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Proton_antipo_M

Protein Analysis

369

Amino Acids

40.55

Weight (kDa)

10.71

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 92 - 182 4.4e-14 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 252, 566, 842, 1004
AciI CCGC 1 cut(s) 566
AclWI GGATC 2 cut(s) 635, 772
AcoI YGGCCR 1 cut(s) 447
AcsI RAATTY 3 cut(s) 488, 607, 632
AcuI CTGAAG 1 cut(s) 309
AfaI GTAC 2 cut(s) 469, 922
AfiI CCNNNNNNNGG 4 cut(s) 191, 731, 878, 1059
AflIII ACRYGT 1 cut(s) 1002
AgsI TTSAA 1 cut(s) 953
AjiI CACGTC 1 cut(s) 269
AjnI CCWGG 1 cut(s) 463
AloI GAACNNNNNNTCC 2 cut(s) 1054, 1086
AluBI AGCT 6 cut(s) 203, 477, 509, 525, 542, 814
AluI AGCT 6 cut(s) 203, 477, 509, 525, 542, 814
Alw21I GWGCWC 2 cut(s) 407, 1087
Alw26I GTCTC 2 cut(s) 278, 941
AlwI GGATC 2 cut(s) 635, 772
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 3 cut(s) 54, 163, 447
ApeKI GCWGC 4 cut(s) 237, 474, 542, 972
ApoI RAATTY 3 cut(s) 488, 607, 632
Asp700I GAANNNNTTC 1 cut(s) 1004
AspLEI GCGC 4 cut(s) 547, 566, 844, 852
AspS9I GGNCC 4 cut(s) 54, 163, 182, 882
AsuC2I CCSGG 1 cut(s) 977
AsuHPI GGTGA 2 cut(s) 453, 1042
AvaI CYCGRG 1 cut(s) 111
AvaII GGWCC 2 cut(s) 182, 882
BalI TGGCCA 1 cut(s) 449
BauI CACGAG 1 cut(s) 1086
Bbv12I GWGCWC 2 cut(s) 407, 1087
BbvI GCAGC 4 cut(s) 249, 486, 529, 959
BccI CCATC 2 cut(s) 503, 700
BceAI ACGGC 2 cut(s) 41, 856
BcgI CGANNNNNNTGC 2 cut(s) 463, 497
BciT130I CCWGG 1 cut(s) 465
BcnI CCSGG 1 cut(s) 977
BcoDI GTCTC 2 cut(s) 278, 941
BfaI CTAG 1 cut(s) 471
BfoI RGCGCY 1 cut(s) 853
BisI GCNGC 5 cut(s) 238, 475, 543, 567, 973
BlpI GCTNAGC 1 cut(s) 867
BlsI GCNGC 5 cut(s) 239, 476, 544, 568, 974
Bme1390I CCNGG 2 cut(s) 465, 977
Bme18I GGWCC 2 cut(s) 182, 882
BmeT110I CYCGRG 1 cut(s) 111
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 4 cut(s) 54, 163, 182, 882
BmiI GGNNCC 4 cut(s) 215, 321, 807, 883
BmrFI CCNGG 2 cut(s) 465, 977
BmsI GCATC 1 cut(s) 705
BplI GAGNNNNNCTC 2 cut(s) 65, 97
Bpu1102I GCTNAGC 1 cut(s) 867
BpuMI CCSGG 1 cut(s) 977
BsaJI CCNNGG 2 cut(s) 112, 940
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 731, 878, 1059
Bse1I ACTGG 1 cut(s) 321
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 2 cut(s) 112, 940
BseGI GGATG 2 cut(s) 558, 720
BseLI CCNNNNNNNGG 4 cut(s) 191, 731, 878, 1059
BseMII CTCAG 2 cut(s) 741, 858
BseNI ACTGG 1 cut(s) 321
BseRI GAGGAG 1 cut(s) 1104
BseXI GCAGC 4 cut(s) 249, 486, 529, 959
Bsh1236I CGCG 4 cut(s) 252, 566, 842, 1004
Bsh1285I CGRYCG 2 cut(s) 256, 696
BshFI GGCC 3 cut(s) 56, 165, 449
BsiEI CGRYCG 2 cut(s) 256, 696
BsiHKAI GWGCWC 2 cut(s) 407, 1087
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 1 cut(s) 976
BslFI GGGAC 4 cut(s) 723, 747, 895, 958
BslI CCNNNNNNNGG 4 cut(s) 191, 731, 878, 1059
BsmAI GTCTC 2 cut(s) 278, 941
BsmBI CGTCTC 1 cut(s) 278
BsmFI GGGAC 4 cut(s) 723, 747, 895, 958
BsmI GAATGC 2 cut(s) 291, 974
BsnI GGCC 3 cut(s) 56, 165, 449
BsoBI CYCGRG 1 cut(s) 111
Bsp1286I GDGCHC 2 cut(s) 407, 1087
Bsp143I GATC 3 cut(s) 253, 640, 777
Bsp1720I GCTNAGC 1 cut(s) 867
BspACI CCGC 1 cut(s) 566
BspANI GGCC 3 cut(s) 56, 165, 449
BspCNI CTCAG 2 cut(s) 740, 859
BspFNI CGCG 4 cut(s) 252, 566, 842, 1004
BspHI TCATGA 1 cut(s) 964
BspLI GGNNCC 4 cut(s) 215, 321, 807, 883
BspPI GGATC 2 cut(s) 635, 772
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 2 cut(s) 112, 940
BssMI GATC 3 cut(s) 253, 640, 777
BssSI CACGAG 1 cut(s) 1086
Bst2BI CACGAG 1 cut(s) 1086
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 2 cut(s) 697, 949
Bst6I CTCTTC 3 cut(s) 66, 102, 442
BstC8I GCNNGC 2 cut(s) 702, 840
BstDEI CTNAG 4 cut(s) 499, 526, 727, 867
BstENI CCTNNNNNAGG 1 cut(s) 189
BstF5I GGATG 2 cut(s) 558, 720
BstFNI CGCG 4 cut(s) 252, 566, 842, 1004
BstH2I RGCGCY 1 cut(s) 853
BstHHI GCGC 4 cut(s) 547, 566, 844, 852
BstKTI GATC 3 cut(s) 256, 643, 780
BstMAI GTCTC 2 cut(s) 278, 941
BstMBI GATC 3 cut(s) 253, 640, 777
BstMCI CGRYCG 2 cut(s) 256, 696
BstMWI GCNNNNNNNGC 1 cut(s) 539
BstNI CCWGG 1 cut(s) 465
BstSCI CCNGG 2 cut(s) 463, 975
BstUI CGCG 4 cut(s) 252, 566, 842, 1004
BstV1I GCAGC 4 cut(s) 249, 486, 529, 959
BsuRI GGCC 3 cut(s) 56, 165, 449
BtgZI GCGATG 1 cut(s) 719
BtrI CACGTC 1 cut(s) 269
BtsCI GGATG 2 cut(s) 558, 720
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 2 cut(s) 702, 840
CciI TCATGA 1 cut(s) 964
CfoI GCGC 4 cut(s) 547, 566, 844, 852
Cfr13I GGNCC 4 cut(s) 54, 163, 182, 882
CsiI ACCWGGT 1 cut(s) 463
Csp6I GTAC 2 cut(s) 468, 921
CviAII CATG 7 cut(s) 64, 311, 409, 913, 917, 965, 1054
CviQI GTAC 2 cut(s) 468, 921
DdeI CTNAG 4 cut(s) 499, 526, 727, 867
DpnI GATC 3 cut(s) 255, 642, 779
DpnII GATC 3 cut(s) 253, 640, 777
DraI TTTAAA 1 cut(s) 612
EaeI YGGCCR 1 cut(s) 447
Eam1104I CTCTTC 3 cut(s) 66, 102, 442
EarI CTCTTC 3 cut(s) 66, 102, 442
Eco47I GGWCC 2 cut(s) 182, 882
Eco57I CTGAAG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 111
EcoNI CCTNNNNNAGG 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 463
EcoT22I ATGCAT 1 cut(s) 918
Esp3I CGTCTC 1 cut(s) 278
FaeI CATG 7 cut(s) 67, 314, 412, 916, 920, 968, 1057
FalI AAGNNNNNCTT 2 cut(s) 803, 835
FaqI GGGAC 4 cut(s) 723, 747, 895, 958
FatI CATG 7 cut(s) 63, 310, 408, 912, 916, 964, 1053
Fnu4HI GCNGC 5 cut(s) 238, 475, 543, 567, 973
FokI GGATG 2 cut(s) 565, 727
Fsp4HI GCNGC 5 cut(s) 238, 475, 543, 567, 973
FspBI CTAG 1 cut(s) 471
GlaI GCGC 4 cut(s) 546, 565, 843, 851
GluI GCNGC 5 cut(s) 238, 475, 543, 567, 973
HaeII RGCGCY 1 cut(s) 853
HaeIII GGCC 3 cut(s) 56, 165, 449
HapII CCGG 1 cut(s) 976
HhaI GCGC 4 cut(s) 547, 566, 844, 852
Hin1II CATG 7 cut(s) 67, 314, 412, 916, 920, 968, 1057
Hin6I GCGC 4 cut(s) 545, 564, 842, 850
HinP1I GCGC 4 cut(s) 545, 564, 842, 850
HindIII AAGCTT 1 cut(s) 812
HinfI GANTC 6 cut(s) 76, 370, 415, 961, 1028, 1063
HpaII CCGG 1 cut(s) 976
HphI GGTGA 2 cut(s) 453, 1042
Hpy166II GTNNAC 2 cut(s) 61, 461
Hpy188I TCNGA 3 cut(s) 278, 640, 941
Hpy188III TCNNGA 4 cut(s) 111, 175, 603, 965
Hpy8I GTNNAC 2 cut(s) 61, 461
Hpy99I CGWCG 1 cut(s) 273
HpyAV CCTTC 4 cut(s) 226, 333, 442, 1057
HpyCH4III ACNGT 2 cut(s) 697, 949
HpyCH4IV ACGT 3 cut(s) 15, 268, 856
HpyCH4V TGCA 2 cut(s) 756, 916
HpyF10VI GCNNNNNNNGC 1 cut(s) 539
HpyF3I CTNAG 4 cut(s) 499, 526, 727, 867
HpySE526I ACGT 3 cut(s) 15, 268, 856
Hsp92II CATG 7 cut(s) 67, 314, 412, 916, 920, 968, 1057
HspAI GCGC 4 cut(s) 545, 564, 842, 850
Kzo9I GATC 3 cut(s) 253, 640, 777
LmnI GCTCC 3 cut(s) 200, 506, 805
LpnPI CCDG 6 cut(s) 302, 450, 477, 588, 830, 989
Lsp1109I GCAGC 4 cut(s) 249, 486, 529, 959
LweI GCATC 1 cut(s) 705
MabI ACCWGGT 1 cut(s) 463
MaeI CTAG 1 cut(s) 471
MaeII ACGT 3 cut(s) 15, 268, 856
MaeIII GTNAC 2 cut(s) 264, 1037
MalI GATC 3 cut(s) 255, 642, 779
MboI GATC 3 cut(s) 253, 640, 777
MboII GAAGA 4 cut(s) 83, 84, 89, 429
MhlI GDGCHC 2 cut(s) 407, 1087
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 4 cut(s) 279, 488, 607, 632
MluI ACGCGT 1 cut(s) 1002
MluNI TGGCCA 1 cut(s) 449
MlyI GAGTC 2 cut(s) 364, 1057
MmeI TCCRAC 2 cut(s) 664, 961
Mox20I TGGCCA 1 cut(s) 449
Mph1103I ATGCAT 1 cut(s) 918
MroXI GAANNNNTTC 1 cut(s) 1004
MscI TGGCCA 1 cut(s) 449
MseI TTAA 2 cut(s) 305, 611
MslI CAYNNNNRTG 1 cut(s) 1105
Msp20I TGGCCA 1 cut(s) 449
MspI CCGG 1 cut(s) 976
MspR9I CCNGG 2 cut(s) 465, 977
Mva1269I GAATGC 2 cut(s) 291, 974
MvaI CCWGG 1 cut(s) 465
MvnI CGCG 4 cut(s) 252, 566, 842, 1004
MwoI GCNNNNNNNGC 1 cut(s) 539
NciI CCSGG 1 cut(s) 977
NdeII GATC 3 cut(s) 253, 640, 777
NlaIII CATG 7 cut(s) 67, 314, 412, 916, 920, 968, 1057
NlaIV GGNNCC 4 cut(s) 215, 321, 807, 883
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 264
NsiI ATGCAT 1 cut(s) 918
PagI TCATGA 1 cut(s) 964
PctI GAATGC 2 cut(s) 291, 974
PdmI GAANNNNTTC 1 cut(s) 1004
PfeI GAWTC 4 cut(s) 76, 415, 961, 1028
PkrI GCNGC 5 cut(s) 239, 476, 544, 568, 974
Ple19I CGATCG 1 cut(s) 256
PleI GAGTC 2 cut(s) 364, 1057
PpsI GAGTC 2 cut(s) 364, 1057
PpuMI RGGWCCY 1 cut(s) 182
Psp5II RGGWCCY 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 463
PspGI CCWGG 1 cut(s) 463
PspN4I GGNNCC 4 cut(s) 215, 321, 807, 883
PspPI GGNCC 4 cut(s) 54, 163, 182, 882
PspPPI RGGWCCY 1 cut(s) 182
PvuI CGATCG 1 cut(s) 256
RsaI GTAC 2 cut(s) 469, 922
RsaNI GTAC 2 cut(s) 468, 921
RseI CAYNNNNRTG 1 cut(s) 1105
SaqAI TTAA 2 cut(s) 305, 611
SatI GCNGC 5 cut(s) 238, 475, 543, 567, 973
Sau3AI GATC 3 cut(s) 253, 640, 777
Sau96I GGNCC 4 cut(s) 54, 163, 182, 882
SchI GAGTC 2 cut(s) 364, 1057
ScrFI CCNGG 2 cut(s) 465, 977
SduI GDGCHC 2 cut(s) 407, 1087
SexAI ACCWGGT 1 cut(s) 463
SfaNI GCATC 1 cut(s) 705
SinI GGWCC 2 cut(s) 182, 882
SmiMI CAYNNNNRTG 1 cut(s) 1105
Sse9I AATT 4 cut(s) 279, 488, 607, 632
SsiI CCGC 1 cut(s) 566
SspMI CTAG 1 cut(s) 471
StyD4I CCNGG 2 cut(s) 463, 975
TaaI ACNGT 2 cut(s) 697, 949
TaiI ACGT 3 cut(s) 18, 271, 859
TaqI TCGA 3 cut(s) 41, 373, 998
TaqII GACCGA 3 cut(s) 375, 899, 998
TasI AATT 4 cut(s) 279, 488, 607, 632
TauI GCSGC 1 cut(s) 569
TfiI GAWTC 4 cut(s) 76, 415, 961, 1028
Tru1I TTAA 2 cut(s) 305, 611
Tru9I TTAA 2 cut(s) 305, 611
TscAI CASTG 1 cut(s) 405
TseFI GTSAC 1 cut(s) 264
TseI GCWGC 4 cut(s) 237, 474, 542, 972
Tsp45I GTSAC 1 cut(s) 264
TspDTI ATGAA 3 cut(s) 80, 953, 981
TspGWI ACGGA 3 cut(s) 405, 727, 1075
TspRI CASTG 1 cut(s) 405
VpaK11BI GGWCC 2 cut(s) 182, 882
XagI CCTNNNNNAGG 1 cut(s) 189
XapI RAATTY 3 cut(s) 488, 607, 632
XmnI GAANNNNTTC 1 cut(s) 1004
XspI CTAG 1 cut(s) 471
Zsp2I ATGCAT 1 cut(s) 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.