RCHIOBHM_MTG0498681

Proton-conducting membrane transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0015226
Physical Location & Seq
Reverse (-)
140886 .. 141488
603 bp
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UTR
Exon/CDS
Intron
PRQ15632

Sequence Viewer

Length: 516 bp
ATGCTATTAGCTATTCTGTTGATTCTTCTCCAAACGGGAACCACCGATTTACAAATATCATTAACCACAGAATTTAGTGAGCGGCGCCAAATCTTTCTATGGATTGCTTCTTTCGCCTCTTTCGCCGTCAAAGTGCCTATGGTACCAGTTCATATTTGGTTACCCGAAGCTCATGTAGAGGCACCCACGGCAGGATCCGTCATCTTGGCAGGAATTCCTTTAAAATTGGGAACCCACGGGTTTTTAAGATTTTCAATACCCATGTTTCCCGAAGCGACACTTTGTTCCACTCCTTTCATTTATACTCCAAGCGCGATTGCTATAATATATACTTCCTTGACCACTTCAAGACAGATCGATCTTAAGAAGATCATTGCTTACTCCTCAGTAGCCCATATGAATCTGGTGACTATTGGTATGTTTAGTCGGGCGGCGGCCGTTAGGTCACCTATTTTGAGTTATGGACACACAAGGCCAAAACATGTGTGCCGGGCGTGCGACCCATCAACCTACTAG

Protein Analysis

171

Amino Acids

18.79

Weight (kDa)

9.37

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 1 - 143 1.2e-36 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 142
AccB1I GGYRCC 3 cut(s) 84, 142, 181
AccBSI CCGCTC 1 cut(s) 82
AccII CGCG 1 cut(s) 314
AciI CCGC 3 cut(s) 82, 431, 434
AclWI GGATC 2 cut(s) 189, 202
AcoI YGGCCR 1 cut(s) 435
AcsI RAATTY 2 cut(s) 71, 213
AcyI GRCGYC 1 cut(s) 85
AfaI GTAC 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 191
AflII CTTAAG 1 cut(s) 362
AflIII ACRYGT 1 cut(s) 481
AgsI TTSAA 2 cut(s) 255, 348
AluBI AGCT 2 cut(s) 11, 170
AluI AGCT 2 cut(s) 11, 170
AlwI GGATC 2 cut(s) 189, 202
AoxI GGCC 2 cut(s) 435, 473
ApoI RAATTY 2 cut(s) 71, 213
Asp718I GGTACC 1 cut(s) 142
AspLEI GCGC 2 cut(s) 87, 314
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 2 cut(s) 418, 438
BamHI GGATCC 1 cut(s) 194
BanI GGYRCC 3 cut(s) 84, 142, 181
BccI CCATC 1 cut(s) 511
BceAI ACGGC 3 cut(s) 110, 204, 422
BcnI CCSGG 1 cut(s) 491
BfaI CTAG 1 cut(s) 514
BfoI RGCGCY 1 cut(s) 88
BfrI CTTAAG 1 cut(s) 362
BisI GCNGC 3 cut(s) 83, 432, 435
BlsI GCNGC 3 cut(s) 84, 433, 436
Bme1390I CCNGG 1 cut(s) 491
BmiI GGNNCC 6 cut(s) 40, 86, 144, 183, 196, 232
BmrFI CCNGG 1 cut(s) 491
BpuMI CCSGG 1 cut(s) 491
Bsa29I ATCGAT 1 cut(s) 357
BsaHI GRCGYC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 186, 235
Bsc4I CCNNNNNNNGG 1 cut(s) 191
Bse1I ACTGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 372
BseCI ATCGAT 1 cut(s) 357
BseDI CCNNGG 2 cut(s) 186, 235
BseLI CCNNNNNNNGG 1 cut(s) 191
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 146
BseRI GAGGAG 1 cut(s) 373
BseX3I CGGCCG 1 cut(s) 435
Bsh1236I CGCG 1 cut(s) 314
Bsh1285I CGRYCG 1 cut(s) 438
BshFI GGCC 2 cut(s) 437, 475
BshNI GGYRCC 3 cut(s) 84, 142, 181
BshVI ATCGAT 1 cut(s) 357
BsiEI CGRYCG 1 cut(s) 438
BsiSI CCGG 1 cut(s) 490
BslI CCNNNNNNNGG 1 cut(s) 191
BsnI GGCC 2 cut(s) 437, 475
Bsp143I GATC 4 cut(s) 194, 354, 358, 369
BspACI CCGC 3 cut(s) 82, 431, 434
BspANI GGCC 2 cut(s) 437, 475
BspCNI CTCAG 1 cut(s) 398
BspDI ATCGAT 1 cut(s) 357
BspFNI CGCG 1 cut(s) 314
BspLI GGNNCC 6 cut(s) 40, 86, 144, 183, 196, 232
BspPI GGATC 2 cut(s) 189, 202
BspT107I GGYRCC 3 cut(s) 84, 142, 181
BspTI CTTAAG 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 1 cut(s) 146
BssECI CCNNGG 2 cut(s) 186, 235
BssMI GATC 4 cut(s) 194, 354, 358, 369
BssNI GRCGYC 1 cut(s) 85
BstACI GRCGYC 1 cut(s) 85
BstAFI CTTAAG 1 cut(s) 362
BstC8I GCNNGC 1 cut(s) 496
BstDEI CTNAG 1 cut(s) 385
BstDSI CCRYGG 2 cut(s) 186, 235
BstEII GGTNACC 2 cut(s) 159, 444
BstFNI CGCG 1 cut(s) 314
BstH2I RGCGCY 1 cut(s) 88
BstHHI GCGC 2 cut(s) 87, 314
BstKTI GATC 4 cut(s) 197, 357, 361, 372
BstMBI GATC 4 cut(s) 194, 354, 358, 369
BstMCI CGRYCG 1 cut(s) 438
BstMWI GCNNNNNNNGC 4 cut(s) 113, 122, 188, 495
BstNSI RCATGY 1 cut(s) 485
BstPI GGTNACC 2 cut(s) 159, 444
BstSCI CCNGG 1 cut(s) 489
BstUI CGCG 1 cut(s) 314
BstX2I RGATCY 1 cut(s) 194
BstYI RGATCY 1 cut(s) 194
BstZI CGGCCG 1 cut(s) 435
Bsu15I ATCGAT 1 cut(s) 357
BsuRI GGCC 2 cut(s) 437, 475
BsuTUI ATCGAT 1 cut(s) 357
BtgI CCRYGG 2 cut(s) 186, 235
Cac8I GCNNGC 1 cut(s) 496
CfoI GCGC 2 cut(s) 87, 314
ClaI ATCGAT 1 cut(s) 357
Csp6I GTAC 1 cut(s) 143
CviAII CATG 3 cut(s) 173, 262, 482
CviJI RGCY 5 cut(s) 11, 170, 392, 437, 475
CviKI_1 RGCY 5 cut(s) 11, 170, 392, 437, 475
CviQI GTAC 1 cut(s) 143
DdeI CTNAG 1 cut(s) 385
DinI GGCGCC 1 cut(s) 86
DpnI GATC 4 cut(s) 196, 356, 360, 371
DpnII GATC 4 cut(s) 194, 354, 358, 369
DraI TTTAAA 1 cut(s) 222
EaeI YGGCCR 1 cut(s) 435
EagI CGGCCG 1 cut(s) 435
EclXI CGGCCG 1 cut(s) 435
Eco52I CGGCCG 1 cut(s) 435
Eco91I GGTNACC 2 cut(s) 159, 444
EcoO65I GGTNACC 2 cut(s) 159, 444
EcoRI GAATTC 1 cut(s) 213
EgeI GGCGCC 1 cut(s) 86
EheI GGCGCC 1 cut(s) 86
FaeI CATG 3 cut(s) 176, 265, 485
FalI AAGNNNNNCTT 2 cut(s) 264, 296
FatI CATG 3 cut(s) 172, 261, 481
FauNDI CATATG 1 cut(s) 396
Fnu4HI GCNGC 3 cut(s) 83, 432, 435
Fsp4HI GCNGC 3 cut(s) 83, 432, 435
FspBI CTAG 1 cut(s) 514
GlaI GCGC 2 cut(s) 86, 313
GluI GCNGC 3 cut(s) 83, 432, 435
HaeII RGCGCY 1 cut(s) 88
HaeIII GGCC 2 cut(s) 437, 475
HapII CCGG 1 cut(s) 490
HhaI GCGC 2 cut(s) 87, 314
Hin1I GRCGYC 1 cut(s) 85
Hin1II CATG 3 cut(s) 176, 265, 485
Hin6I GCGC 2 cut(s) 85, 312
HinP1I GCGC 2 cut(s) 85, 312
HinfI GANTC 2 cut(s) 22, 400
HpaII CCGG 1 cut(s) 490
HphI GGTGA 2 cut(s) 418, 438
Hpy188III TCNNGA 2 cut(s) 269, 348
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 122, 188, 495
HpyF3I CTNAG 1 cut(s) 385
Hsp92I GRCGYC 1 cut(s) 85
Hsp92II CATG 3 cut(s) 176, 265, 485
HspAI GCGC 2 cut(s) 85, 312
KasI GGCGCC 1 cut(s) 84
KpnI GGTACC 1 cut(s) 146
Kzo9I GATC 4 cut(s) 194, 354, 358, 369
LpnPI CCDG 5 cut(s) 159, 177, 195, 389, 503
MaeI CTAG 1 cut(s) 514
MaeIII GTNAC 3 cut(s) 159, 406, 444
MalI GATC 4 cut(s) 196, 356, 360, 371
MbiI CCGCTC 1 cut(s) 82
MboI GATC 4 cut(s) 194, 354, 358, 369
MboII GAAGA 2 cut(s) 17, 379
MflI RGATCY 1 cut(s) 194
MluCI AATT 3 cut(s) 71, 213, 224
Mly113I GGCGCC 1 cut(s) 85
MnlI CCTC 3 cut(s) 127, 172, 394
MseI TTAA 4 cut(s) 62, 221, 245, 363
MspCI CTTAAG 1 cut(s) 362
MspI CCGG 1 cut(s) 490
MspR9I CCNGG 1 cut(s) 491
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 4 cut(s) 113, 122, 188, 495
NarI GGCGCC 1 cut(s) 85
NciI CCSGG 1 cut(s) 491
NdeI CATATG 1 cut(s) 396
NdeII GATC 4 cut(s) 194, 354, 358, 369
NlaIII CATG 3 cut(s) 176, 265, 485
NlaIV GGNNCC 6 cut(s) 40, 86, 144, 183, 196, 232
NmuCI GTSAC 2 cut(s) 406, 444
NspI RCATGY 1 cut(s) 485
PciI ACATGT 1 cut(s) 481
PfeI GAWTC 2 cut(s) 22, 400
PkrI GCNGC 3 cut(s) 84, 433, 436
PluTI GGCGCC 1 cut(s) 88
PscI ACATGT 1 cut(s) 481
PspEI GGTNACC 2 cut(s) 159, 444
PspN4I GGNNCC 6 cut(s) 40, 86, 144, 183, 196, 232
PsuI RGATCY 1 cut(s) 194
RsaI GTAC 1 cut(s) 144
RsaNI GTAC 1 cut(s) 143
SaqAI TTAA 4 cut(s) 62, 221, 245, 363
SatI GCNGC 3 cut(s) 83, 432, 435
Sau3AI GATC 4 cut(s) 194, 354, 358, 369
ScrFI CCNGG 1 cut(s) 491
SetI ASST 5 cut(s) 13, 172, 446, 451, 512
SfoI GGCGCC 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 3 cut(s) 71, 213, 224
SsiI CCGC 3 cut(s) 82, 431, 434
SspDI GGCGCC 1 cut(s) 84
SspMI CTAG 1 cut(s) 514
StyD4I CCNGG 1 cut(s) 489
TaqI TCGA 1 cut(s) 357
TasI AATT 3 cut(s) 71, 213, 224
TauI GCSGC 3 cut(s) 85, 434, 437
TfiI GAWTC 2 cut(s) 22, 400
Tru1I TTAA 4 cut(s) 62, 221, 245, 363
Tru9I TTAA 4 cut(s) 62, 221, 245, 363
TseFI GTSAC 2 cut(s) 406, 444
Tsp45I GTSAC 2 cut(s) 406, 444
TspDTI ATGAA 3 cut(s) 140, 286, 413
TspGWI ACGGA 1 cut(s) 187
Vha464I CTTAAG 1 cut(s) 362
XapI RAATTY 2 cut(s) 71, 213
XceI RCATGY 1 cut(s) 485
XcmI CCANNNNNNNNNTGG 1 cut(s) 153
XspI CTAG 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.