pycom12g00020

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004704
Physical Location & Seq
Reverse (-)
31390 .. 35558
4169 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 597 bp
ATGTTTGCTTCTCAAGTATCAAGAATTGGAGTTTTGATACTGAAACAGCTCTCCAGAGGCAAATCCGCATCTATAACCAGAGCAGGATATACTAGTAATTCTTACAGACATTACTTGCAACCTCTGTTTGATCAACAATCTGAAACTAATTTGTTGCGAGGAACCATCTTTGAGAAGCATCGTCTTTTTTCTACCAGTAGTGCCAGCCATTCCTCGAATGAAGGAATTGAGCAGAAAGAAAAGATATCTGTGACATTTGTTGATAAGGATGGAGAGGAAAAACATATCAAGGTTCCTACTGGAATGTCTATGCTTGAAGCTGCCCATGAAAATGATATAGAACTTGAAGGAGCGTGTGAAGGTTCACTTGCCTGTTCAACATGTCATGTGATTGTGATGGACGTGGACTATTACAGCAAATTAGAAGACCCAACTGATGAGGAAAATGATATGTTGGACTTGGCCTTTGGACTCACTGAAACGTCTCGCTTGGGTTGTCAAGTCATTGCAAAACCTGAACTTGATGGAATTCGTTTAGCAATTCCAGCTGCCACCCGAAACTTTGCTGTCGATGGCTATGTTCCAAAACCACACTAG

Protein Analysis

199

Amino Acids

21.89

Weight (kDa)

5.24

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 88 - 171 1.5e-12 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 66
AcsI RAATTY 1 cut(s) 528
AflIII ACRYGT 1 cut(s) 380
AgsI TTSAA 3 cut(s) 317, 347, 378
AhlI ACTAGT 1 cut(s) 92
AjiI CACGTC 1 cut(s) 403
AluBI AGCT 3 cut(s) 49, 320, 548
AluI AGCT 3 cut(s) 49, 320, 548
Alw26I GTCTC 1 cut(s) 489
AoxI GGCC 1 cut(s) 462
ApeKI GCWGC 2 cut(s) 320, 548
ApoI RAATTY 1 cut(s) 528
ArsI GACNNNNNNTTYG 2 cut(s) 449, 481
BbsI GAAGAC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 307, 535
BccI CCATC 5 cut(s) 173, 263, 391, 518, 566
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 489
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 2 cut(s) 93, 595
BisI GCNGC 2 cut(s) 321, 549
BlsI GCNGC 2 cut(s) 322, 550
BmgBI CACGTC 1 cut(s) 403
BmiI GGNNCC 2 cut(s) 163, 294
BmsI GCATC 2 cut(s) 77, 187
BpiI GAAGAC 1 cut(s) 432
BpmI CTGGAG 1 cut(s) 37
Bse1I ACTGG 2 cut(s) 195, 304
Bse3DI GCAATG 1 cut(s) 504
BseGI GGATG 1 cut(s) 274
BseMI GCAATG 1 cut(s) 504
BseNI ACTGG 2 cut(s) 195, 304
BseXI GCAGC 2 cut(s) 307, 535
BshFI GGCC 1 cut(s) 464
BsmAI GTCTC 1 cut(s) 489
BsmBI CGTCTC 1 cut(s) 489
BsnI GGCC 1 cut(s) 464
Bsp143I GATC 1 cut(s) 130
BspACI CCGC 1 cut(s) 66
BspANI GGCC 1 cut(s) 464
BspLI GGNNCC 2 cut(s) 163, 294
BsrDI GCAATG 1 cut(s) 504
BsrI ACTGG 2 cut(s) 195, 304
BssMI GATC 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 205
BstF5I GGATG 1 cut(s) 274
BstKTI GATC 1 cut(s) 133
BstMAI GTCTC 1 cut(s) 489
BstMBI GATC 1 cut(s) 130
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNSI RCATGY 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 307, 535
BstV2I GAAGAC 1 cut(s) 432
BsuRI GGCC 1 cut(s) 464
BtrI CACGTC 1 cut(s) 403
BtsCI GGATG 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 474
Cac8I GCNNGC 1 cut(s) 205
CviAII CATG 3 cut(s) 326, 381, 386
CviJI RGCY 6 cut(s) 49, 207, 320, 464, 548, 576
CviKI_1 RGCY 6 cut(s) 49, 207, 320, 464, 548, 576
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
Eco32I GATATC 1 cut(s) 246
EcoRI GAATTC 1 cut(s) 528
EcoRV GATATC 1 cut(s) 246
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 3 cut(s) 329, 384, 389
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FatI CATG 3 cut(s) 325, 380, 385
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 321, 549
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 2 cut(s) 321, 549
FspBI CTAG 2 cut(s) 93, 595
GluI GCNGC 2 cut(s) 321, 549
GsuI CTGGAG 1 cut(s) 37
HaeIII GGCC 1 cut(s) 464
Hin1II CATG 3 cut(s) 329, 384, 389
HinfI GANTC 1 cut(s) 471
Hpy166II GTNNAC 2 cut(s) 365, 406
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 2 cut(s) 21, 54
Hpy8I GTNNAC 2 cut(s) 365, 406
HpyAV CCTTC 3 cut(s) 215, 341, 353
HpyCH4IV ACGT 2 cut(s) 402, 482
HpyCH4V TGCA 2 cut(s) 118, 509
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpySE526I ACGT 2 cut(s) 402, 482
Hsp92II CATG 3 cut(s) 329, 384, 389
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 1 cut(s) 130
LmnI GCTCC 1 cut(s) 350
LpnPI CCDG 9 cut(s) 67, 69, 91, 208, 217, 285, 385, 528, 558
Lsp1109I GCAGC 2 cut(s) 307, 535
LweI GCATC 2 cut(s) 77, 187
MaeI CTAG 2 cut(s) 93, 595
MaeII ACGT 2 cut(s) 402, 482
MaeIII GTNAC 1 cut(s) 250
MalI GATC 1 cut(s) 132
MboI GATC 1 cut(s) 130
MboII GAAGA 1 cut(s) 437
MluCI AATT 7 cut(s) 24, 97, 148, 225, 419, 528, 540
MlyI GAGTC 1 cut(s) 465
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 6 cut(s) 50, 132, 152, 223, 268, 433
MslI CAYNNNNRTG 1 cut(s) 330
MspA1I CMGCKG 1 cut(s) 548
MwoI GCNNNNNNNGC 1 cut(s) 545
NdeII GATC 1 cut(s) 130
NlaIII CATG 3 cut(s) 329, 384, 389
NlaIV GGNNCC 2 cut(s) 163, 294
NmuCI GTSAC 1 cut(s) 250
NspI RCATGY 1 cut(s) 384
PciI ACATGT 1 cut(s) 380
PkrI GCNGC 2 cut(s) 322, 550
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
PscI ACATGT 1 cut(s) 380
PspN4I GGNNCC 2 cut(s) 163, 294
PvuII CAGCTG 1 cut(s) 548
RseI CAYNNNNRTG 1 cut(s) 330
SatI GCNGC 2 cut(s) 321, 549
Sau3AI GATC 1 cut(s) 130
SchI GAGTC 1 cut(s) 465
SetI ASST 9 cut(s) 51, 124, 294, 322, 364, 405, 485, 517, 550
SfaNI GCATC 2 cut(s) 77, 187
SmiMI CAYNNNNRTG 1 cut(s) 330
SmlI CTYRAG 1 cut(s) 12
SmoI CTYRAG 1 cut(s) 12
SpeI ACTAGT 1 cut(s) 92
Sse9I AATT 7 cut(s) 24, 97, 148, 225, 419, 528, 540
SsiI CCGC 1 cut(s) 66
SspMI CTAG 2 cut(s) 93, 595
TaiI ACGT 2 cut(s) 405, 485
TaqI TCGA 2 cut(s) 215, 570
TasI AATT 7 cut(s) 24, 97, 148, 225, 419, 528, 540
TscAI CASTG 1 cut(s) 481
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 320, 548
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 234, 342
TspRI CASTG 1 cut(s) 481
XapI RAATTY 1 cut(s) 528
XceI RCATGY 1 cut(s) 384
XspI CTAG 2 cut(s) 93, 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.