pycom12g09100

WW domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002830
Physical Location & Seq
Forward (+)
10951689 .. 10961022
9334 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g09100.4

Sequence Viewer

Length: 2085 bp
ATGATTAGGAGTCCATCAACGGTGAGGTGGGCGACGTCTAGAGGAAACTCGCGGAGGCGAGGTGTGAGTGAGACGGCGTGGTGGTGTATATCGGTGAGAGCACAAAGAAGAGTGTGGAGGGGTGTACGTGTACAGTGTATTATTCAGGGGAGCGAAGATTGGCTGTGCTCACAAAGCCTCTGCCCACCCTGTGCCTGCATCCTTCTTTCGACAGCTCTCCGAGAGTTGGGAGTGGGCCTTGGTTTGTGGATCTGGATTTGGTTTAAGGACATTCGGCCCAAAGAAAATAAACGCTTCCGCTCGGCCGTCTCCATCCAGCCCCTTCCCTTCCCCTCCCTTCCCTTCCTGCTCAAATTTCCAAACAATCGACAGACCGGTGTTCCCTGCAGGCTGCAATTTGTGATTCTCTCTGTCTCTGTCTCTGTCTCTGTCTCCGTCTCTCTATCTGCATACAAACAGGTGCAGCAGCAGCAGAGAGGGATTTTGGTGTGTCTGACTCCTCTGGCTATGGAGAGTTCCCATTCCCAAGATGACCGTGAGCCACCCCCTCCGGGCGTCGACTCCTTTCCACAATATTCCAACTCAACCCATCACTTTACTACCCACAGCCAGACTTTCACCCCACACCCACACCAACAGCCGCAGCCGCAGGGTGCGTTTTGGGCCCAAGATTCTACTGCAAACTCCGCGTTGGATATCGAAAATGCTAGCCAAATTGATGTGTCATTGCATCAACAAGAAATTTCTACTCAAAATGTCATACGTATTCAGAGAGAAGCAAGAGGCGTGAGTCTGTCTTCTAAGGATGGTTTAGACGTCTTCTCCCAACGCCTTGATCCTAATGCTCTAAAGGAGCATCTACTGAAGATGACCATGCAACATCGTGCTCAAATGGCTTCAAAACGTGGCAAGTCTTCCGTTCCTGGAGAAGGTAACATAGAAATTGGTAATGGCTATGGTGTACCCGGAGGAGGTGCTTATTATGGTGCTCCAGGACCTAATGTAACTGCACCTAGAAATCTGGGAGTTGGAAATAGTGAGGTGGATCAGAAGAATATTGAATCTGATAAAGAGTTAAAACAGAAGCCTCCGGTTAAAGAATTGCCAGAGTATCTTAAACAAAAGTTGAGGGATAGAGGTATTCTTAAAGATGATTCAAATAATGGCAATATTAAGTTGGAAAGGAGCTCAGCTCAACAAATGGAACACGGGAAATTACCTTCTGGTTGGGTGGAGGCAACAGATCCTGCAAGTGGTGCTTTGTATTATTATAACAAGACCACAGGCAAGACTCAATGGGAAAAGCCTGCTCACACATCTTCTGTTACACCACTTCAGTCGCCTTTATCTCTTGCAGAAGATTGGGTGGAGGCTTTGGATGAAACAACAGGTCACAAGTATTACTACAATACAAAGACACAAGTATCGCAATGGAAACACCCTGGGTCATCAGAGCAGATTGCATCCCAACATTTTGATAGTATGGTTTCTGGAAACCCTGCTAATGTTTATTGGGATGGCCAATCAACTGAGGTACAAGCAGGAAAGGATGAGTCGTCCACACTAAAGAAATGCCTGAGTTGCGGTGGATGGGGAGTAGGACTTGTTCAGATGTGGGGCTACTGCAATCATTGTACAAGAGTTCTTGATCTTCCACAAAGCCAGTACATGATGACTAGTTTGAGTAATTATCAGCAGACCCAAAATCCTGTAGATACAAAAGGTGATTCAGACAGAAAGGCTCCCACGCAGAGGTCTAATTGGAAACCTCCAATGGCAAAAGGAAATAGAAAGGATAGTAAGAAACGGGCTTACGATGAGGATGATGAATTGGATCCAATGGACCCCAGTGCATATTCAGATGCTCCTCGTGGTGGCTGGGTAGTAGGCCTCAAAGGAGTGCAGCCACGAGCAGCTGATACCACTGCCACAGGTCCCCTTTTTCAACAGCGGCCTTACCCATCACCAGGTGCTGTCTTGAGAAAAAATGCTGAAATTGCTTCGCAGACCAAGAAGCCAAGCTCGCAATTTGCACCTATATCCAAAAGAGGGGACGGCAGTGATGGACTTGGCGATGCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000375 GO:0000377 GO:0000380 GO:0000398 GO:0001817 GO:0001819 GO:0002218 GO:0002230 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002697 GO:0002831 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003713 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0008380 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0014070 GO:0016043 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0019219 GO:0019222 GO:0022008 GO:0030030 GO:0030154 GO:0030182 GO:0031175 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032101 GO:0032479 GO:0032481 GO:0032501 GO:0032502 GO:0034641 GO:0042221 GO:0043021 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043330 GO:0043331 GO:0043484 GO:0043900 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045088 GO:0045089 GO:0045935 GO:0046483 GO:0048468 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050688 GO:0050691 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071359 GO:0071360 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090304 GO:0097159 GO:0098542 GO:0120036 GO:0140110 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WW

Protein Analysis

695

Amino Acids

76.79

Weight (kDa)

8.97

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1272
AatII GACGTC 2 cut(s) 38, 819
AccBSI CCGCTC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 558
AccII CGCG 2 cut(s) 52, 689
AciI CCGC 7 cut(s) 52, 298, 641, 647, 687, 1584, 1951
AclWI GGATC 6 cut(s) 257, 830, 1053, 1238, 1829, 1842
AcoI YGGCCR 2 cut(s) 303, 1519
AcsI RAATTY 2 cut(s) 353, 741
AcuI CTGAAG 2 cut(s) 884, 1319
AcyI GRCGYC 3 cut(s) 35, 555, 816
AdeI CACNNNGTG 2 cut(s) 191, 1970
AfaI GTAC 6 cut(s) 126, 132, 963, 1536, 1636, 1667
AfiI CCNNNNNNNGG 9 cut(s) 226, 551, 929, 971, 1253, 1752, 1874, 1967, 2049
AflIII ACRYGT 1 cut(s) 127
AgeI ACCGGT 1 cut(s) 374
AgsI TTSAA 4 cut(s) 900, 1061, 1158, 1946
AhlI ACTAGT 1 cut(s) 1676
AjnI CCWGG 4 cut(s) 922, 991, 1441, 1966
AluBI AGCT 5 cut(s) 215, 1188, 1193, 1916, 2022
AluI AGCT 5 cut(s) 215, 1188, 1193, 1916, 2022
Alw21I GWGCWC 5 cut(s) 103, 170, 889, 991, 1190
Alw26I GTCTC 7 cut(s) 65, 313, 418, 424, 430, 436, 442
AlwI GGATC 6 cut(s) 257, 830, 1053, 1238, 1829, 1842
AlwNI CAGNNNCTG 2 cut(s) 1247, 1973
AoxI GGCC 7 cut(s) 235, 275, 303, 663, 1519, 1888, 1952
ApaI GGGCCC 1 cut(s) 667
ApeKI GCWGC 7 cut(s) 391, 463, 466, 469, 643, 1903, 1913
ApoI RAATTY 2 cut(s) 353, 741
AsiGI ACCGGT 1 cut(s) 374
AspS9I GGNCC 7 cut(s) 235, 276, 663, 664, 995, 1843, 1934
AsuC2I CCSGG 2 cut(s) 552, 966
AsuHPI GGTGA 5 cut(s) 34, 106, 610, 1736, 1956
AsuNHI GCTAGC 1 cut(s) 707
AvaII GGWCC 3 cut(s) 995, 1843, 1934
BaeGI GKGCMC 1 cut(s) 667
BaeI ACNNNNGTAYC 2 cut(s) 1407, 1440
BalI TGGCCA 1 cut(s) 1521
BamHI GGATCC 1 cut(s) 1834
BanII GRGCYC 2 cut(s) 667, 1190
BauI CACGAG 2 cut(s) 1869, 1908
BbsI GAAGAC 3 cut(s) 789, 811, 906
Bbv12I GWGCWC 5 cut(s) 103, 170, 889, 991, 1190
BbvI GCAGC 7 cut(s) 378, 475, 478, 481, 655, 1915, 1925
BccI CCATC 8 cut(s) 22, 320, 597, 800, 1511, 1584, 1969, 2057
BceAI ACGGC 3 cut(s) 90, 290, 2071
BciT130I CCWGG 4 cut(s) 924, 993, 1443, 1968
BcnI CCSGG 2 cut(s) 552, 966
BcoDI GTCTC 7 cut(s) 65, 313, 418, 424, 430, 436, 442
BcuI ACTAGT 1 cut(s) 1676
BfaI CTAG 4 cut(s) 39, 708, 1014, 1677
BfmI CTRYAG 2 cut(s) 385, 1710
BlpI GCTNAGC 1 cut(s) 1189
Bme1390I CCNGG 6 cut(s) 552, 924, 966, 993, 1443, 1968
Bme18I GGWCC 3 cut(s) 995, 1843, 1934
BmgT120I GGNCC 7 cut(s) 235, 276, 663, 664, 995, 1843, 1934
BmiI GGNNCC 5 cut(s) 665, 1743, 1836, 1845, 1936
BmrFI CCNGG 6 cut(s) 552, 924, 966, 993, 1443, 1968
BmrI ACTGGG 1 cut(s) 1842
BmsI GCATC 6 cut(s) 207, 739, 865, 1472, 1852, 2065
BmtI GCTAGC 1 cut(s) 711
BmuI ACTGGG 1 cut(s) 1842
BpiI GAAGAC 3 cut(s) 789, 811, 906
BplI GAGNNNNNCTC 2 cut(s) 1177, 1209
BpmI CTGGAG 2 cut(s) 945, 975
Bpu1102I GCTNAGC 1 cut(s) 1189
BpuEI CTTGAG 1 cut(s) 1999
BpuMI CCSGG 2 cut(s) 552, 966
BsaAI YACGTR 2 cut(s) 128, 764
BsaHI GRCGYC 3 cut(s) 35, 555, 816
BsaJI CCNNGG 3 cut(s) 238, 1441, 1442
BsaWI WCCGGW 2 cut(s) 374, 1090
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 806, 836
Bsc4I CCNNNNNNNGG 9 cut(s) 226, 551, 929, 971, 1253, 1752, 1874, 1967, 2049
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 2 cut(s) 1663, 1848
Bse3DI GCAATG 2 cut(s) 725, 1436
BseBI CCWGG 4 cut(s) 924, 993, 1443, 1968
BseDI CCNNGG 3 cut(s) 238, 1441, 1442
BseGI GGATG 9 cut(s) 198, 312, 811, 1384, 1463, 1522, 1555, 1595, 1828
BseLI CCNNNNNNNGG 9 cut(s) 226, 551, 929, 971, 1253, 1752, 1874, 1967, 2049
BseMI GCAATG 2 cut(s) 725, 1436
BseMII CTCAG 3 cut(s) 1203, 1521, 1568
BseNI ACTGG 2 cut(s) 1663, 1848
BseRI GAGGAG 3 cut(s) 489, 984, 1857
BseSI GKGCMC 1 cut(s) 667
BseX3I CGGCCG 1 cut(s) 303
BseXI GCAGC 7 cut(s) 378, 475, 478, 481, 655, 1915, 1925
BseYI CCCAGC 1 cut(s) 1878
BsgI GTGCAG 3 cut(s) 482, 993, 1922
Bsh1236I CGCG 2 cut(s) 52, 689
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 7 cut(s) 237, 277, 305, 665, 1521, 1890, 1954
BshTI ACCGGT 1 cut(s) 374
BsiEI CGRYCG 1 cut(s) 306
BsiHKAI GWGCWC 5 cut(s) 103, 170, 889, 991, 1190
BsiSI CCGG 4 cut(s) 375, 551, 966, 1091
BslFI GGGAC 2 cut(s) 1920, 2066
BslI CCNNNNNNNGG 9 cut(s) 226, 551, 929, 971, 1253, 1752, 1874, 1967, 2049
BsmAI GTCTC 7 cut(s) 65, 313, 418, 424, 430, 436, 442
BsmBI CGTCTC 3 cut(s) 65, 313, 442
BsmFI GGGAC 2 cut(s) 1920, 2066
BsnI GGCC 7 cut(s) 237, 277, 305, 665, 1521, 1890, 1954
Bsp120I GGGCCC 1 cut(s) 663
Bsp1286I GDGCHC 6 cut(s) 103, 170, 667, 889, 991, 1190
Bsp1407I TGTACA 2 cut(s) 130, 1634
Bsp143I GATC 6 cut(s) 249, 835, 1045, 1243, 1648, 1834
Bsp1720I GCTNAGC 1 cut(s) 1189
BspACI CCGC 7 cut(s) 52, 298, 641, 647, 687, 1584, 1951
BspANI GGCC 7 cut(s) 237, 277, 305, 665, 1521, 1890, 1954
BspCNI CTCAG 3 cut(s) 1202, 1522, 1569
BspFNI CGCG 2 cut(s) 52, 689
BspLI GGNNCC 5 cut(s) 665, 1743, 1836, 1845, 1936
BspMAI CTGCAG 1 cut(s) 389
BspOI GCTAGC 1 cut(s) 711
BspPI GGATC 6 cut(s) 257, 830, 1053, 1238, 1829, 1842
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 2 cut(s) 725, 1436
BsrFI RCCGGY 1 cut(s) 374
BsrGI TGTACA 2 cut(s) 130, 1634
BsrI ACTGG 2 cut(s) 1663, 1848
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 238, 1441, 1442
BssMI GATC 6 cut(s) 249, 835, 1045, 1243, 1648, 1834
BssNI GRCGYC 3 cut(s) 35, 555, 816
BssSI CACGAG 2 cut(s) 1869, 1908
BssT1I CCWWGG 1 cut(s) 238
Bst2BI CACGAG 2 cut(s) 1869, 1908
Bst2UI CCWGG 4 cut(s) 924, 993, 1443, 1968
Bst4CI ACNGT 3 cut(s) 22, 135, 536
Bst6I CTCTTC 1 cut(s) 103
BstACI GRCGYC 3 cut(s) 35, 555, 816
BstAPI GCANNNNNTGC 1 cut(s) 1256
BstAUI TGTACA 2 cut(s) 130, 1634
BstBAI YACGTR 2 cut(s) 128, 764
BstC8I GCNNGC 5 cut(s) 196, 389, 709, 1308, 2024
BstDEI CTNAG 4 cut(s) 801, 1189, 1530, 1577
BstENI CCTNNNNNAGG 1 cut(s) 927
BstF5I GGATG 9 cut(s) 198, 312, 811, 1384, 1463, 1522, 1555, 1595, 1828
BstFNI CGCG 2 cut(s) 52, 689
BstKTI GATC 6 cut(s) 252, 838, 1048, 1246, 1651, 1837
BstMAI GTCTC 7 cut(s) 65, 313, 418, 424, 430, 436, 442
BstMBI GATC 6 cut(s) 249, 835, 1045, 1243, 1648, 1834
BstMCI CGRYCG 1 cut(s) 306
BstNI CCWGG 4 cut(s) 924, 993, 1443, 1968
BstSCI CCNGG 6 cut(s) 550, 922, 964, 991, 1441, 1966
BstSFI CTRYAG 2 cut(s) 385, 1710
BstSLI GKGCMC 1 cut(s) 667
BstSNI TACGTA 1 cut(s) 764
BstUI CGCG 2 cut(s) 52, 689
BstV1I GCAGC 7 cut(s) 378, 475, 478, 481, 655, 1915, 1925
BstV2I GAAGAC 3 cut(s) 789, 811, 906
BstX2I RGATCY 3 cut(s) 249, 1243, 1834
BstYI RGATCY 3 cut(s) 249, 1243, 1834
BstZI CGGCCG 1 cut(s) 303
BsuRI GGCC 7 cut(s) 237, 277, 305, 665, 1521, 1890, 1954
BtsCI GGATG 9 cut(s) 198, 312, 811, 1384, 1463, 1522, 1555, 1595, 1828
BtsI GCAGTG 2 cut(s) 1923, 2065
BtsIMutI CAGTG 4 cut(s) 140, 1855, 1923, 2065
Cac8I GCNNGC 5 cut(s) 196, 389, 709, 1308, 2024
CaiI CAGNNNCTG 2 cut(s) 1247, 1973
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 7 cut(s) 235, 276, 663, 664, 995, 1843, 1934
CseI GACGC 1 cut(s) 544
CsiI ACCWGGT 1 cut(s) 1966
Csp6I GTAC 6 cut(s) 125, 131, 962, 1535, 1635, 1666
CspAI ACCGGT 1 cut(s) 374
CviAII CATG 2 cut(s) 874, 1669
CviQI GTAC 6 cut(s) 125, 131, 962, 1535, 1635, 1666
DdeI CTNAG 4 cut(s) 801, 1189, 1530, 1577
DpnI GATC 6 cut(s) 251, 837, 1047, 1245, 1650, 1836
DpnII GATC 6 cut(s) 249, 835, 1045, 1243, 1648, 1834
DraIII CACNNNGTG 2 cut(s) 191, 1970
EaeI YGGCCR 2 cut(s) 303, 1519
EagI CGGCCG 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 1188
EclXI CGGCCG 1 cut(s) 303
Eco105I TACGTA 1 cut(s) 764
Eco130I CCWWGG 1 cut(s) 238
Eco147I AGGCCT 1 cut(s) 1890
Eco24I GRGCYC 2 cut(s) 667, 1190
Eco32I GATATC 1 cut(s) 697
Eco47I GGWCC 3 cut(s) 995, 1843, 1934
Eco52I CGGCCG 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 1188
Eco57I CTGAAG 2 cut(s) 884, 1319
EcoICRI GAGCTC 1 cut(s) 1188
EcoNI CCTNNNNNAGG 1 cut(s) 927
EcoO109I RGGNCCY 2 cut(s) 995, 1934
EcoRII CCWGG 4 cut(s) 922, 991, 1441, 1966
EcoRV GATATC 1 cut(s) 697
EcoT14I CCWWGG 1 cut(s) 238
EcoT38I GRGCYC 2 cut(s) 667, 1190
ErhI CCWWGG 1 cut(s) 238
Esp3I CGTCTC 3 cut(s) 65, 313, 442
FaeI CATG 2 cut(s) 877, 1672
FalI AAGNNNNNCTT 2 cut(s) 1243, 1275
FaqI GGGAC 2 cut(s) 1920, 2066
FatI CATG 2 cut(s) 873, 1668
FblI GTMKAC 1 cut(s) 558
FokI GGATG 9 cut(s) 185, 299, 818, 1391, 1450, 1529, 1562, 1602, 1835
FriOI GRGCYC 2 cut(s) 667, 1190
FspBI CTAG 4 cut(s) 39, 708, 1014, 1677
GsaI CCCAGC 1 cut(s) 1882
GsuI CTGGAG 2 cut(s) 945, 975
HaeIII GGCC 7 cut(s) 237, 277, 305, 665, 1521, 1890, 1954
HapII CCGG 4 cut(s) 375, 551, 966, 1091
HgaI GACGC 1 cut(s) 544
Hin1I GRCGYC 3 cut(s) 35, 555, 816
Hin1II CATG 2 cut(s) 877, 1672
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HpaII CCGG 4 cut(s) 375, 551, 966, 1091
HphI GGTGA 5 cut(s) 34, 106, 610, 1736, 1956
Hpy166II GTNNAC 5 cut(s) 125, 131, 559, 962, 1560
Hpy188I TCNGA 9 cut(s) 221, 495, 771, 1050, 1066, 1453, 1611, 1732, 1861
Hpy188III TCNNGA 5 cut(s) 39, 253, 1491, 1646, 1978
Hpy8I GTNNAC 5 cut(s) 125, 131, 559, 962, 1560
Hpy99I CGWCG 2 cut(s) 37, 560
HpyAV CCTTC 7 cut(s) 212, 332, 337, 347, 352, 923, 1230
HpyCH4III ACNGT 3 cut(s) 22, 135, 536
HpyCH4IV ACGT 5 cut(s) 35, 127, 763, 816, 904
HpyF3I CTNAG 4 cut(s) 801, 1189, 1530, 1577
HpySE526I ACGT 5 cut(s) 35, 127, 763, 816, 904
Hsp92I GRCGYC 3 cut(s) 35, 555, 816
Hsp92II CATG 2 cut(s) 877, 1672
Kzo9I GATC 6 cut(s) 249, 835, 1045, 1243, 1648, 1834
LmnI GCTCC 6 cut(s) 150, 853, 994, 1185, 1747, 1870
Lsp1109I GCAGC 7 cut(s) 378, 475, 478, 481, 655, 1915, 1925
LweI GCATC 6 cut(s) 207, 739, 865, 1472, 1852, 2065
MabI ACCWGGT 1 cut(s) 1966
MaeI CTAG 4 cut(s) 39, 708, 1014, 1677
MaeII ACGT 5 cut(s) 35, 127, 763, 816, 904
MaeIII GTNAC 4 cut(s) 932, 1003, 1324, 1391
MalI GATC 6 cut(s) 251, 837, 1047, 1245, 1650, 1836
MbiI CCGCTC 1 cut(s) 300
MboI GATC 6 cut(s) 249, 835, 1045, 1243, 1648, 1834
MflI RGATCY 3 cut(s) 249, 1243, 1834
MhlI GDGCHC 6 cut(s) 103, 170, 667, 889, 991, 1190
MlsI TGGCCA 1 cut(s) 1521
MluNI TGGCCA 1 cut(s) 1521
MlyI GAGTC 6 cut(s) 19, 490, 554, 799, 1285, 1562
MmeI TCCRAC 4 cut(s) 603, 672, 1009, 1158
Mox20I TGGCCA 1 cut(s) 1521
MscI TGGCCA 1 cut(s) 1521
MseI TTAA 6 cut(s) 264, 1076, 1095, 1116, 1146, 1173
Msp20I TGGCCA 1 cut(s) 1521
MspA1I CMGCKG 2 cut(s) 1916, 1951
MspI CCGG 4 cut(s) 375, 551, 966, 1091
MspR9I CCNGG 6 cut(s) 552, 924, 966, 993, 1443, 1968
MvaI CCWGG 4 cut(s) 924, 993, 1443, 1968
MvnI CGCG 2 cut(s) 52, 689
NciI CCSGG 2 cut(s) 552, 966
NdeII GATC 6 cut(s) 249, 835, 1045, 1243, 1648, 1834
NheI GCTAGC 1 cut(s) 707
NlaIII CATG 2 cut(s) 877, 1672
NlaIV GGNNCC 5 cut(s) 665, 1743, 1836, 1845, 1936
NmeAIII GCCGAG 1 cut(s) 281
NmuCI GTSAC 1 cut(s) 1391
PasI CCCWGGG 1 cut(s) 1442
PceI AGGCCT 1 cut(s) 1890
PfeI GAWTC 5 cut(s) 403, 671, 1061, 1154, 1727
PfoI TCCNGGA 2 cut(s) 922, 991
PinAI ACCGGT 1 cut(s) 374
PleI GAGTC 6 cut(s) 18, 490, 554, 798, 1285, 1561
PpsI GAGTC 6 cut(s) 18, 490, 554, 798, 1285, 1561
Ppu21I YACGTR 2 cut(s) 128, 764
PpuMI RGGWCCY 2 cut(s) 995, 1934
PsiI TTATAA 1 cut(s) 1272
Psp124BI GAGCTC 1 cut(s) 1190
Psp5II RGGWCCY 2 cut(s) 995, 1934
Psp6I CCWGG 4 cut(s) 922, 991, 1441, 1966
PspFI CCCAGC 1 cut(s) 1878
PspGI CCWGG 4 cut(s) 922, 991, 1441, 1966
PspN4I GGNNCC 5 cut(s) 665, 1743, 1836, 1845, 1936
PspOMI GGGCCC 1 cut(s) 663
PspPI GGNCC 7 cut(s) 235, 276, 663, 664, 995, 1843, 1934
PspPPI RGGWCCY 2 cut(s) 995, 1934
PsrI GAACNNNNNNTAC 2 cut(s) 1590, 1622
PstI CTGCAG 1 cut(s) 389
PstNI CAGNNNCTG 2 cut(s) 1247, 1973
PsuI RGATCY 3 cut(s) 249, 1243, 1834
PvuII CAGCTG 1 cut(s) 1916
RsaI GTAC 6 cut(s) 126, 132, 963, 1536, 1636, 1667
RsaNI GTAC 6 cut(s) 125, 131, 962, 1535, 1635, 1666
SacI GAGCTC 1 cut(s) 1190
SalI GTCGAC 1 cut(s) 557
SaqAI TTAA 6 cut(s) 264, 1076, 1095, 1116, 1146, 1173
Sau3AI GATC 6 cut(s) 249, 835, 1045, 1243, 1648, 1834
Sau96I GGNCC 7 cut(s) 235, 276, 663, 664, 995, 1843, 1934
SbfI CCTGCAGG 1 cut(s) 389
SchI GAGTC 6 cut(s) 19, 490, 554, 799, 1285, 1562
ScrFI CCNGG 6 cut(s) 552, 924, 966, 993, 1443, 1968
SdaI CCTGCAGG 1 cut(s) 389
SduI GDGCHC 6 cut(s) 103, 170, 667, 889, 991, 1190
SexAI ACCWGGT 1 cut(s) 1966
SfaNI GCATC 6 cut(s) 207, 739, 865, 1472, 1852, 2065
SfcI CTRYAG 2 cut(s) 385, 1710
SinI GGWCC 3 cut(s) 995, 1843, 1934
SmlI CTYRAG 1 cut(s) 1978
SmoI CTYRAG 1 cut(s) 1978
SnaBI TACGTA 1 cut(s) 764
SpeI ACTAGT 1 cut(s) 1676
Sse8387I CCTGCAGG 1 cut(s) 389
SseBI AGGCCT 1 cut(s) 1890
SsiI CCGC 7 cut(s) 52, 298, 641, 647, 687, 1584, 1951
SspI AATATT 3 cut(s) 575, 1057, 1171
SspMI CTAG 4 cut(s) 39, 708, 1014, 1677
SstI GAGCTC 1 cut(s) 1190
StuI AGGCCT 1 cut(s) 1890
StyD4I CCNGG 6 cut(s) 550, 922, 964, 991, 1441, 1966
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 3 cut(s) 22, 135, 536
TaiI ACGT 5 cut(s) 38, 130, 766, 819, 907
TaqI TCGA 4 cut(s) 209, 367, 558, 699
TatI WGTACW 3 cut(s) 130, 1634, 1665
TauI GCSGC 3 cut(s) 643, 649, 1954
TfiI GAWTC 5 cut(s) 403, 671, 1061, 1154, 1727
Tru1I TTAA 6 cut(s) 264, 1076, 1095, 1116, 1146, 1173
Tru9I TTAA 6 cut(s) 264, 1076, 1095, 1116, 1146, 1173
TscAI CASTG 4 cut(s) 140, 1855, 1930, 2065
TseFI GTSAC 1 cut(s) 1391
TseI GCWGC 7 cut(s) 391, 463, 466, 469, 643, 1903, 1913
Tsp45I GTSAC 1 cut(s) 1391
TspDTI ATGAA 2 cut(s) 1395, 1842
TspGWI ACGGA 2 cut(s) 424, 907
TspRI CASTG 4 cut(s) 140, 1855, 1930, 2065
VpaK11BI GGWCC 3 cut(s) 995, 1843, 1934
XagI CCTNNNNNAGG 1 cut(s) 927
XapI RAATTY 2 cut(s) 353, 741
XbaI TCTAGA 1 cut(s) 38
XmiI GTMKAC 1 cut(s) 558
XspI CTAG 4 cut(s) 39, 708, 1014, 1677
ZraI GACGTC 2 cut(s) 36, 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.