pycom12g10740

ZINC FINGER protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015025
Physical Location & Seq
Reverse (-)
12790955 .. 12791641
687 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g10740.1

Sequence Viewer

Length: 687 bp
ATGGCAAACTGTTTGATTCTTCTGGCGCAAGGCAATTCTACCCCAAAACCCCAAAACCCTAATTTGGTTAACAACAATAAGGCGGCTTCAGCAGCAGCTTCGGCCGGGTTATATTTGTATCAGTGCAAGACGTGTGACCGGTGCTTCCCGTCGTTTCAAGCGCTTGGTGGACATAGAGCCAGTCACAAGAAACCCGGTAAGCAGGCTAACATTCACAGCACAATTCAAGACAATAAGAAAGCGTTGTCATTCGTGGAAGAAGTGGAGGAATTTGATCTGTTTAACAACACCAGCACCACGTTGTCATTACAAATTTCGAATAGGGCTTTCAGGGCATTGAACAGTGATACTACTAGTCCTTGTTATAACAAAAACAAAGCCAATAAGGTTCATGAGTGTTCGGTGTGTGGCGCGGAGTTCGCATCTGGTCAAGCTCTAGGGGGTCATATGAGAAGGCATAGAACATTAATCAGCACAGCAGCAACAACAGCAGCAGCGGAAGCCATGAGTAGTCATCCTCAGTCCCAATCGGAATCAAAGAGACCACGAAATGTTTTGCAGTTGGATCTTAATCTTCCTGCGCCGGAAGAAGAGCACCTGCAGGAAACCAATAAGTTTCCCTTTGGAGCCAAGAAGGAACAAGTCATCGTCTTCTCTGCCTCTCCTTTGGTGGGTTGCCAATACTAA

Protein Analysis

229

Amino Acids

24.78

Weight (kDa)

8.91

Isoelectric Point (pI)

59.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_6 PF13912 40 - 65 1.5e-09 C2H2-type zinc finger
zf-C2H2_6 PF13912 130 - 154 1.6e-12 C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015025)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03510
fragaria_vesca FvH4_6g16820
malus_domestica MD04G1089100.v1.1 MD12G1107100.v1.1
prunus_persica Prupe.7G125800_v2.0.a1
pyrus_communis pycom12g10740
rosa_chinensis RchiOBHm_Chr3g0470831
rosa_laevigata RLG00000024242
rosa_multiflora Rmu_sc0002790.1_g000023
rosa_roxburghii Rroxscaffold_6G00410500
rosa_rugosa Rorug03G0113100
rosa_samantha Rh3AG162700 Rh3BG187800 Rh3CG177800 Rh3DG188000
rosa_wichuraiana Rw3G015320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
AarI CACCTGC 1 cut(s) 606
Acc36I ACCTGC 1 cut(s) 606
AccII CGCG 1 cut(s) 413
AciI CCGC 3 cut(s) 83, 413, 497
AclWI GGATC 1 cut(s) 573
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 2 cut(s) 269, 312
AcuI CTGAAG 1 cut(s) 72
AfeI AGCGCT 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 64, 671
AflIII ACRYGT 1 cut(s) 131
AgeI ACCGGT 1 cut(s) 138
AgsI TTSAA 3 cut(s) 158, 227, 340
AhlI ACTAGT 1 cut(s) 353
AjiI CACGTC 1 cut(s) 132
AluBI AGCT 2 cut(s) 98, 434
AluI AGCT 2 cut(s) 98, 434
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 1 cut(s) 535
AlwI GGATC 1 cut(s) 573
Aor51HI AGCGCT 1 cut(s) 162
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 5 cut(s) 92, 95, 479, 491, 494
ApoI RAATTY 2 cut(s) 269, 312
AseI ATTAAT 1 cut(s) 467
AsiGI ACCGGT 1 cut(s) 138
AspLEI GCGC 4 cut(s) 28, 163, 413, 583
AsuC2I CCSGG 2 cut(s) 106, 195
AsuII TTCGAA 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 643
Bbv12I GWGCWC 1 cut(s) 597
BbvI GCAGC 5 cut(s) 104, 107, 491, 503, 506
BcgI CGANNNNNNTGC 2 cut(s) 81, 115
BcnI CCSGG 2 cut(s) 106, 195
BcoDI GTCTC 1 cut(s) 535
BcuI ACTAGT 1 cut(s) 353
BfaI CTAG 2 cut(s) 354, 437
BfmI CTRYAG 1 cut(s) 599
BfoI RGCGCY 1 cut(s) 164
BfuAI ACCTGC 1 cut(s) 606
BisI GCNGC 6 cut(s) 84, 93, 96, 480, 492, 495
BlsI GCNGC 6 cut(s) 85, 94, 97, 481, 493, 496
Bme1390I CCNGG 2 cut(s) 106, 195
BmgBI CACGTC 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 628
BmrFI CCNGG 2 cut(s) 106, 195
BmsI GCATC 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 643
Bpu14I TTCGAA 1 cut(s) 317
BpuMI CCSGG 2 cut(s) 106, 195
BsaBI GATNNNNATC 1 cut(s) 570
BsaI GGTCTC 1 cut(s) 535
BsaWI WCCGGW 1 cut(s) 138
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 671
Bse118I RCCGGY 1 cut(s) 138
Bse1I ACTGG 1 cut(s) 180
Bse8I GATNNNNATC 1 cut(s) 570
BseGI GGATG 1 cut(s) 514
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 2 cut(s) 64, 671
BseMII CTCAG 1 cut(s) 533
BseNI ACTGG 1 cut(s) 180
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 5 cut(s) 104, 107, 491, 503, 506
Bsh1236I CGCG 1 cut(s) 413
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 1 cut(s) 104
BshTI ACCGGT 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 597
BsiSI CCGG 4 cut(s) 105, 139, 195, 584
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 2 cut(s) 64, 671
BsmAI GTCTC 1 cut(s) 535
BsmFI GGGAC 1 cut(s) 508
BsnI GGCC 1 cut(s) 104
Bso31I GGTCTC 1 cut(s) 535
Bsp119I TTCGAA 1 cut(s) 317
Bsp1286I GDGCHC 1 cut(s) 597
Bsp143I GATC 2 cut(s) 274, 565
BspACI CCGC 3 cut(s) 83, 413, 497
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 1 cut(s) 532
BspFNI CGCG 1 cut(s) 413
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 628
BspMAI CTGCAG 1 cut(s) 603
BspMI ACCTGC 1 cut(s) 606
BspPI GGATC 1 cut(s) 573
BspQI GCTCTTC 1 cut(s) 585
BspT104I TTCGAA 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 138
BsrI ACTGG 1 cut(s) 180
BssAI RCCGGY 1 cut(s) 138
BssMI GATC 2 cut(s) 274, 565
Bst4CI ACNGT 2 cut(s) 11, 344
Bst6I CTCTTC 1 cut(s) 585
BstBI TTCGAA 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 519
BstF5I GGATG 1 cut(s) 514
BstFNI CGCG 1 cut(s) 413
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 4 cut(s) 28, 163, 413, 583
BstKTI GATC 2 cut(s) 277, 568
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 2 cut(s) 274, 565
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 7 cut(s) 89, 92, 101, 332, 419, 488, 500
BstSCI CCNGG 2 cut(s) 104, 193
BstSFI CTRYAG 1 cut(s) 599
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 5 cut(s) 104, 107, 491, 503, 506
BstV2I GAAGAC 1 cut(s) 643
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BstZI CGGCCG 1 cut(s) 102
BsuRI GGCC 1 cut(s) 104
BtrI CACGTC 1 cut(s) 132
BtsCI GGATG 1 cut(s) 514
BtsIMutI CAGTG 2 cut(s) 128, 349
BveI ACCTGC 1 cut(s) 606
Cac8I GCNNGC 1 cut(s) 204
CciI TCATGA 1 cut(s) 391
CfoI GCGC 4 cut(s) 28, 163, 413, 583
Cfr10I RCCGGY 1 cut(s) 138
CspAI ACCGGT 1 cut(s) 138
CviAII CATG 2 cut(s) 392, 505
DdeI CTNAG 1 cut(s) 519
DpnI GATC 2 cut(s) 276, 567
DpnII GATC 2 cut(s) 274, 565
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 585
EarI CTCTTC 1 cut(s) 585
EclXI CGGCCG 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 535
Eco47III AGCGCT 1 cut(s) 162
Eco52I CGGCCG 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 72
FaeI CATG 2 cut(s) 395, 508
FaiI YATR 8 cut(s) 112, 174, 366, 393, 447, 449, 459, 506
FalI AAGNNNNNCTT 2 cut(s) 605, 637
FaqI GGGAC 1 cut(s) 508
FatI CATG 2 cut(s) 391, 504
FauNDI CATATG 1 cut(s) 447
Fnu4HI GCNGC 6 cut(s) 84, 93, 96, 480, 492, 495
FokI GGATG 1 cut(s) 501
Fsp4HI GCNGC 6 cut(s) 84, 93, 96, 480, 492, 495
FspBI CTAG 2 cut(s) 354, 437
GlaI GCGC 4 cut(s) 27, 162, 412, 582
GluI GCNGC 6 cut(s) 84, 93, 96, 480, 492, 495
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 1 cut(s) 104
HapII CCGG 4 cut(s) 105, 139, 195, 584
HhaI GCGC 4 cut(s) 28, 163, 413, 583
Hin1II CATG 2 cut(s) 395, 508
Hin6I GCGC 4 cut(s) 26, 161, 411, 581
HinP1I GCGC 4 cut(s) 26, 161, 411, 581
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 2 cut(s) 16, 533
HpaI GTTAAC 1 cut(s) 70
HpaII CCGG 4 cut(s) 105, 139, 195, 584
Hpy166II GTNNAC 2 cut(s) 70, 170
Hpy188I TCNGA 1 cut(s) 532
Hpy188III TCNNGA 2 cut(s) 227, 392
Hpy8I GTNNAC 2 cut(s) 70, 170
Hpy99I CGWCG 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 447, 628
HpyCH4III ACNGT 2 cut(s) 11, 344
HpyCH4IV ACGT 2 cut(s) 131, 299
HpyCH4V TGCA 3 cut(s) 126, 559, 601
HpyF10VI GCNNNNNNNGC 7 cut(s) 89, 92, 101, 332, 419, 488, 500
HpyF3I CTNAG 1 cut(s) 519
HpySE526I ACGT 2 cut(s) 131, 299
Hsp92II CATG 2 cut(s) 395, 508
HspAI GCGC 4 cut(s) 26, 161, 411, 581
KspAI GTTAAC 1 cut(s) 70
Kzo9I GATC 2 cut(s) 274, 565
LguI GCTCTTC 1 cut(s) 585
LmnI GCTCC 1 cut(s) 626
Lsp1109I GCAGC 5 cut(s) 104, 107, 491, 503, 506
LweI GCATC 1 cut(s) 431
MaeI CTAG 2 cut(s) 354, 437
MaeII ACGT 2 cut(s) 131, 299
MaeIII GTNAC 2 cut(s) 134, 182
MalI GATC 2 cut(s) 276, 567
MboI GATC 2 cut(s) 274, 565
MboII GAAGA 6 cut(s) 11, 269, 566, 599, 602, 643
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 1 cut(s) 597
MluCI AATT 5 cut(s) 34, 61, 222, 269, 312
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 3 cut(s) 259, 528, 670
MseI TTAA 4 cut(s) 69, 282, 467, 570
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 4 cut(s) 105, 139, 195, 584
MspR9I CCNGG 2 cut(s) 106, 195
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 7 cut(s) 89, 92, 101, 332, 419, 488, 500
NciI CCSGG 2 cut(s) 106, 195
NdeI CATATG 1 cut(s) 447
NdeII GATC 2 cut(s) 274, 565
NlaIII CATG 2 cut(s) 395, 508
NlaIV GGNNCC 1 cut(s) 628
NmuCI GTSAC 2 cut(s) 134, 182
NspV TTCGAA 1 cut(s) 317
PagI TCATGA 1 cut(s) 391
PaqCI CACCTGC 1 cut(s) 606
PciSI GCTCTTC 1 cut(s) 585
PfeI GAWTC 2 cut(s) 16, 533
PinAI ACCGGT 1 cut(s) 138
PkrI GCNGC 6 cut(s) 85, 94, 97, 481, 493, 496
PshBI ATTAAT 1 cut(s) 467
PsiI TTATAA 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 628
PstI CTGCAG 1 cut(s) 603
PsuI RGATCY 1 cut(s) 565
SapI GCTCTTC 1 cut(s) 585
SaqAI TTAA 4 cut(s) 69, 282, 467, 570
SatI GCNGC 6 cut(s) 84, 93, 96, 480, 492, 495
Sau3AI GATC 2 cut(s) 274, 565
SbfI CCTGCAGG 1 cut(s) 603
ScrFI CCNGG 2 cut(s) 106, 195
SdaI CCTGCAGG 1 cut(s) 603
SduI GDGCHC 1 cut(s) 597
SetI ASST 6 cut(s) 100, 134, 302, 390, 436, 600
SfaNI GCATC 1 cut(s) 431
SfcI CTRYAG 1 cut(s) 599
SfuI TTCGAA 1 cut(s) 317
SpeI ACTAGT 1 cut(s) 353
Sse8387I CCTGCAGG 1 cut(s) 603
Sse9I AATT 5 cut(s) 34, 61, 222, 269, 312
SsiI CCGC 3 cut(s) 83, 413, 497
SspMI CTAG 2 cut(s) 354, 437
StyD4I CCNGG 2 cut(s) 104, 193
TaaI ACNGT 2 cut(s) 11, 344
TaiI ACGT 2 cut(s) 134, 302
TaqI TCGA 1 cut(s) 317
TasI AATT 5 cut(s) 34, 61, 222, 269, 312
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 2 cut(s) 16, 533
Tru1I TTAA 4 cut(s) 69, 282, 467, 570
Tru9I TTAA 4 cut(s) 69, 282, 467, 570
TscAI CASTG 2 cut(s) 128, 349
TseFI GTSAC 2 cut(s) 134, 182
TseI GCWGC 5 cut(s) 92, 95, 479, 491, 494
Tsp45I GTSAC 2 cut(s) 134, 182
TspDTI ATGAA 1 cut(s) 380
TspRI CASTG 2 cut(s) 128, 349
VspI ATTAAT 1 cut(s) 467
XapI RAATTY 2 cut(s) 269, 312
XspI CTAG 2 cut(s) 354, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.