Rorug03G0113100

ankyrin repeat family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0014540
Physical Location & Seq
Reverse (-)
9146918 .. 9151129
4212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0113100.1

Sequence Viewer

Length: 1188 bp
ATGTCGTCCGACGACGAGAGGGAGGAGAAGGAGTTGGATCTCACCTCTCCAGAAGTGGTCACCAAATACAAGAGTGCCGCTGAGATTGTGAACAAGGCTTTGCAGCGAGTGATATCGGAATGCAAGCCGAAAGCTAAGATTGTGGACGTTTGCGAGATAGGTGATTCATACATCAGAGAGCAAACTGGTAACATGTACAAAAATGTGAAGAAGAAGATTGAACGAGGTGTTGCTTTCCCAACTTCCATTTCTGTGAACAACACTGTATGCCATTTCTCTCCACTTGCCAGTGATGAAACAGTGCTCGAAGAGGGGGATATTGTAAAGATTGATATGGGTTGTCATATAGATGGGTTCATTGCTATAGTCGCTCATACTCATGCTCTTCAAGCAGGGCCCATCGCAGGAAGGGCTGCAGATGTCATTGCAGCTGCTAATACTGCTGCTGAAGTTGCCTTGAGGCTTGTAAAGCCGGGAAAAAAGAACAAAGATGTAACAGAGGCTATACAAAAGGTTGCTGCTGCTTATGATTGCAAAATTGTTGAAGGTGTTCTTAGCCACCAGATGAAGCAGTTTGTGATAGATGCAAACAAGGTCATATTGAGTGTATCCAATCCAGAGACAAGAGTTGAGGAGGCAGAATTTGAGGAGAATGAAGTTTATGCAATTGATATAGTAGCAAGCACAGGTGAAGGAAAGCCTAAGTTGCTGGATGAGAAGCAGACAACTATATACAAGAGGGCTGTGGACAAGAGCTATCACCTGAAGATGAAAGCTTCTAGGTTCATTTTCAGTGAAATTAGCCAGAAATTCCCCCTCATGCCATTCACTGCAAGAGCTTTGGAAGAGAAAAGGGCTCGTCTGGGTTTACTTGAGTGTGTCAACCATGAACTTTTGCAGCCATATCCTGTTTTGCATGAGAAGCCTGGTGATTTTGTTGCTCATATCAAGTTCACTGTATTGCTCATGCCAAACGGATCAGACCGGATTACATCTCATCCTCTACAGGAGCTGCAACCCACTAAGCAAGTAGATGATCCTGAAATCAAAGCATGGTTGGCTTTGGGCACAAAGACAAAGAAGAAAGGCGGTGGAAAGAAGAAGAAAGGTAAGAAGGGTGATAAAGCCGAGGAGTCTACCGAAGCTCAACCTATGGATGCTACAAATGGTGCTGAAACTCAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 Ank_5

Protein Analysis

395

Amino Acids

43.48

Weight (kDa)

6.59

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M24 PF00557 22 - 223 2.1e-21 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09890 AT3G09890
fragaria_vesca FvH4_6g16830
malus_domestica MD12G1106800.v1.1
prunus_persica Prupe.7G125700_v2.0.a1
pyrus_communis pycom12g10730
rosa_chinensis RchiOBHm_Chr3g0470841
rosa_laevigata RLG00000024241
rosa_multiflora Rmu_co8290787.1_g000001 Rmu_sc0002790.1_g000024
rosa_roxburghii Rroxscaffold_6G00410490
rosa_rugosa Rorug03G0113100
rosa_samantha Rh3AG162800 Rh3BG187900 Rh3CG177900 Rh3DG187900
rosa_wichuraiana Rw3G015330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1136
AciI CCGC 2 cut(s) 78, 1089
AclWI GGATC 3 cut(s) 45, 985, 1031
AcsI RAATTY 2 cut(s) 641, 809
AcuI CTGAAG 2 cut(s) 468, 785
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 1 cut(s) 404
AflIII ACRYGT 1 cut(s) 192
AgsI TTSAA 3 cut(s) 221, 389, 545
AjnI CCWGG 1 cut(s) 925
AjuI GAANNNNNNNTTGG 2 cut(s) 232, 264
AluBI AGCT 7 cut(s) 134, 431, 756, 776, 839, 1012, 1145
AluI AGCT 7 cut(s) 134, 431, 756, 776, 839, 1012, 1145
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 614
AlwI GGATC 3 cut(s) 45, 985, 1031
AlwNI CAGNNNCTG 1 cut(s) 1012
AoxI GGCC 1 cut(s) 395
ApaI GGGCCC 1 cut(s) 399
ApeKI GCWGC 9 cut(s) 103, 413, 428, 431, 443, 518, 521, 898, 1012
ApoI RAATTY 2 cut(s) 641, 809
Asp700I GAANNNNTTC 1 cut(s) 549
AspS9I GGNCC 2 cut(s) 395, 396
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 7 cut(s) 34, 52, 173, 701, 752, 941, 1130
BaeGI GKGCMC 2 cut(s) 399, 1070
BanII GRGCYC 2 cut(s) 399, 859
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 9 cut(s) 115, 400, 418, 430, 440, 505, 508, 910, 999
BccI CCATC 2 cut(s) 344, 407
BciT130I CCWGG 1 cut(s) 927
BciVI GTATCC 1 cut(s) 619
BcnI CCSGG 1 cut(s) 474
BcoDI GTCTC 1 cut(s) 614
BfaI CTAG 1 cut(s) 780
BfmI CTRYAG 3 cut(s) 363, 414, 1004
BfuI GTATCC 1 cut(s) 619
Bme1390I CCNGG 2 cut(s) 474, 927
BmgT120I GGNCC 2 cut(s) 395, 396
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 2 cut(s) 474, 927
BmsI GCATC 2 cut(s) 574, 1147
BpmI CTGGAG 1 cut(s) 33
BpuEI CTTGAG 3 cut(s) 478, 893, 1164
BpuMI CCSGG 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 1128
BsaWI WCCGGW 1 cut(s) 984
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 1 cut(s) 404
Bse1I ACTGG 2 cut(s) 190, 288
Bse3DI GCAATG 2 cut(s) 357, 423
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 1 cut(s) 1128
BseGI GGATG 3 cut(s) 718, 997, 1162
BseLI CCNNNNNNNGG 1 cut(s) 404
BseMI GCAATG 2 cut(s) 357, 423
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 190, 288
BseRI GAGGAG 4 cut(s) 38, 647, 662, 1145
BseSI GKGCMC 2 cut(s) 399, 1070
BseXI GCAGC 9 cut(s) 115, 400, 418, 430, 440, 505, 508, 910, 999
BshFI GGCC 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 2 cut(s) 473, 985
BslI CCNNNNNNNGG 1 cut(s) 404
BsmAI GTCTC 1 cut(s) 614
BsmI GAATGC 1 cut(s) 125
BsnI GGCC 1 cut(s) 397
Bsp120I GGGCCC 1 cut(s) 395
Bsp1286I GDGCHC 4 cut(s) 306, 399, 859, 1070
Bsp1407I TGTACA 1 cut(s) 195
Bsp143I GATC 3 cut(s) 37, 977, 1036
BspACI CCGC 2 cut(s) 78, 1089
BspANI GGCC 1 cut(s) 397
BspCNI CTCAG 1 cut(s) 73
BspLI GGNNCC 1 cut(s) 397
BspMAI CTGCAG 1 cut(s) 418
BspPI GGATC 3 cut(s) 45, 985, 1031
BspQI GCTCTTC 1 cut(s) 390
BsrDI GCAATG 2 cut(s) 357, 423
BsrGI TGTACA 1 cut(s) 195
BsrI ACTGG 2 cut(s) 190, 288
BssECI CCNNGG 1 cut(s) 1128
BssMI GATC 3 cut(s) 37, 977, 1036
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 3 cut(s) 265, 301, 958
Bst6I CTCTTC 3 cut(s) 303, 390, 840
BstAUI TGTACA 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 125, 682
BstDEI CTNAG 5 cut(s) 81, 135, 554, 702, 1023
BstEII GGTNACC 1 cut(s) 58
BstF5I GGATG 3 cut(s) 718, 997, 1162
BstKTI GATC 3 cut(s) 40, 980, 1039
BstMAI GTCTC 1 cut(s) 614
BstMBI GATC 3 cut(s) 37, 977, 1036
BstMWI GCNNNNNNNGC 9 cut(s) 368, 389, 410, 440, 452, 469, 706, 922, 1058
BstNI CCWGG 1 cut(s) 927
BstNSI RCATGY 1 cut(s) 196
BstPI GGTNACC 1 cut(s) 58
BstSCI CCNGG 2 cut(s) 472, 925
BstSFI CTRYAG 3 cut(s) 363, 414, 1004
BstSLI GKGCMC 2 cut(s) 399, 1070
BstV1I GCAGC 9 cut(s) 115, 400, 418, 430, 440, 505, 508, 910, 999
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuI GTATCC 1 cut(s) 619
BsuRI GGCC 1 cut(s) 397
BtgZI GCGATG 1 cut(s) 385
BtsCI GGATG 3 cut(s) 718, 997, 1162
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 6 cut(s) 261, 295, 306, 799, 828, 954
Cac8I GCNNGC 2 cut(s) 125, 682
CaiI CAGNNNCTG 1 cut(s) 1012
Cfr13I GGNCC 2 cut(s) 395, 396
Csp6I GTAC 1 cut(s) 196
CviAII CATG 7 cut(s) 193, 380, 820, 887, 917, 967, 1053
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 5 cut(s) 81, 135, 554, 702, 1023
DpnI GATC 3 cut(s) 39, 979, 1038
DpnII GATC 3 cut(s) 37, 977, 1036
Eam1104I CTCTTC 3 cut(s) 303, 390, 840
EarI CTCTTC 3 cut(s) 303, 390, 840
Eco24I GRGCYC 2 cut(s) 399, 859
Eco32I GATATC 1 cut(s) 114
Eco57I CTGAAG 2 cut(s) 468, 785
Eco91I GGTNACC 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 395
EcoO65I GGTNACC 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 925
EcoRV GATATC 1 cut(s) 114
EcoT38I GRGCYC 2 cut(s) 399, 859
FaeI CATG 7 cut(s) 196, 383, 823, 890, 920, 970, 1056
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FatI CATG 7 cut(s) 192, 379, 819, 886, 916, 966, 1052
FblI GTMKAC 1 cut(s) 1136
FokI GGATG 3 cut(s) 725, 984, 1169
FriOI GRGCYC 2 cut(s) 399, 859
FspBI CTAG 1 cut(s) 780
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 1 cut(s) 397
HapII CCGG 2 cut(s) 473, 985
Hin1II CATG 7 cut(s) 196, 383, 823, 890, 920, 970, 1056
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HindIII AAGCTT 1 cut(s) 774
HinfI GANTC 2 cut(s) 164, 1133
HpaII CCGG 2 cut(s) 473, 985
HphI GGTGA 7 cut(s) 34, 52, 173, 701, 752, 941, 1130
Hpy166II GTNNAC 8 cut(s) 91, 145, 256, 748, 869, 883, 954, 1137
Hpy188I TCNGA 4 cut(s) 10, 118, 176, 982
Hpy188III TCNNGA 3 cut(s) 50, 617, 1040
Hpy8I GTNNAC 8 cut(s) 91, 145, 256, 748, 869, 883, 954, 1137
Hpy99I CGWCG 2 cut(s) 14, 17
HpyAV CCTTC 5 cut(s) 22, 402, 539, 686, 1108
HpyCH4III ACNGT 3 cut(s) 265, 301, 958
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 9 cut(s) 368, 389, 410, 440, 452, 469, 706, 922, 1058
HpyF3I CTNAG 5 cut(s) 81, 135, 554, 702, 1023
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 7 cut(s) 196, 383, 823, 890, 920, 970, 1056
Kzo9I GATC 3 cut(s) 37, 977, 1036
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 1 cut(s) 1009
Lsp1109I GCAGC 9 cut(s) 115, 400, 418, 430, 440, 505, 508, 910, 999
LweI GCATC 2 cut(s) 574, 1147
MaeI CTAG 1 cut(s) 780
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 3 cut(s) 58, 188, 493
MalI GATC 3 cut(s) 39, 979, 1038
MboI GATC 3 cut(s) 37, 977, 1036
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 4 cut(s) 306, 399, 859, 1070
MluCI AATT 5 cut(s) 537, 641, 666, 798, 809
MlyI GAGTC 1 cut(s) 1142
MmeI TCCRAC 2 cut(s) 15, 33
MroXI GAANNNNTTC 1 cut(s) 549
MslI CAYNNNNRTG 3 cut(s) 251, 348, 378
MspA1I CMGCKG 2 cut(s) 80, 431
MspI CCGG 2 cut(s) 473, 985
MspR9I CCNGG 2 cut(s) 474, 927
MunI CAATTG 1 cut(s) 666
Mva1269I GAATGC 1 cut(s) 125
MvaI CCWGG 1 cut(s) 927
MwoI GCNNNNNNNGC 9 cut(s) 368, 389, 410, 440, 452, 469, 706, 922, 1058
NciI CCSGG 1 cut(s) 474
NdeII GATC 3 cut(s) 37, 977, 1036
NlaIII CATG 7 cut(s) 196, 383, 823, 890, 920, 970, 1056
NlaIV GGNNCC 1 cut(s) 397
NmeAIII GCCGAG 1 cut(s) 1153
NmuCI GTSAC 1 cut(s) 58
NspI RCATGY 1 cut(s) 196
PciI ACATGT 1 cut(s) 192
PciSI GCTCTTC 1 cut(s) 390
PctI GAATGC 1 cut(s) 125
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 1 cut(s) 164
PleI GAGTC 1 cut(s) 1141
PpsI GAGTC 1 cut(s) 1141
PscI ACATGT 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 925
PspEI GGTNACC 1 cut(s) 58
PspGI CCWGG 1 cut(s) 925
PspN4I GGNNCC 1 cut(s) 397
PspOMI GGGCCC 1 cut(s) 395
PspPI GGNCC 2 cut(s) 395, 396
PstI CTGCAG 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 1012
PsuI RGATCY 1 cut(s) 37
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 3 cut(s) 251, 348, 378
SapI GCTCTTC 1 cut(s) 390
Sau3AI GATC 3 cut(s) 37, 977, 1036
Sau96I GGNCC 2 cut(s) 395, 396
SchI GAGTC 1 cut(s) 1142
ScrFI CCNGG 2 cut(s) 474, 927
SduI GDGCHC 4 cut(s) 306, 399, 859, 1070
SfaNI GCATC 2 cut(s) 574, 1147
SfcI CTRYAG 3 cut(s) 363, 414, 1004
SmiMI CAYNNNNRTG 3 cut(s) 251, 348, 378
SmlI CTYRAG 3 cut(s) 457, 872, 1179
SmoI CTYRAG 3 cut(s) 457, 872, 1179
Sse9I AATT 5 cut(s) 537, 641, 666, 798, 809
SsiI CCGC 2 cut(s) 78, 1089
SspMI CTAG 1 cut(s) 780
StyD4I CCNGG 2 cut(s) 472, 925
TaaI ACNGT 3 cut(s) 265, 301, 958
TaiI ACGT 1 cut(s) 150
TaqI TCGA 1 cut(s) 306
TasI AATT 5 cut(s) 537, 641, 666, 798, 809
TatI WGTACW 1 cut(s) 195
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 1 cut(s) 164
TscAI CASTG 6 cut(s) 268, 295, 306, 799, 835, 961
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 9 cut(s) 103, 413, 428, 431, 443, 518, 521, 898, 1012
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 8 cut(s) 156, 309, 346, 581, 669, 775, 785, 903
TspGWI ACGGA 1 cut(s) 990
TspRI CASTG 6 cut(s) 268, 295, 306, 799, 835, 961
XapI RAATTY 2 cut(s) 641, 809
XceI RCATGY 1 cut(s) 196
XmiI GTMKAC 1 cut(s) 1136
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.