MD12G1106800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0014540
Physical Location & Seq
Reverse (-)
16897225 .. 16899767
2543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1106800.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGCGGTTCCCGGGCGGAGGAATGGTCTGATGGAGGAAGAGGACAACGAGGACGAAAACGCTCTGTTCGAGGAAGAGGGTTTGGAGGAGCTTGACGCTCACGCGCCTCCCCACCTTCGCGCCATCGCAGACGCCTCTCAGCAGGGCGACCTCGACGCCCTTCGCCTCGCTCTTGATAACTTAGTAGGCAGCATTGATGATGCAGTGGAAGATGGAGACACAGCTCTCCATCTGGCCTGTCTCTATGGTAATCTTTCTTGTGTCCAGTTACTTTTGGAGAGAGGTGCAAATTTGGAGGCTAAGGATGAAGACGGGGCAATTCCTTTGCATGATGCTTGTGCCGGTGGATACACCGAAGTGGTTCAACTTTTAATTAACAGTGCCAACGATTCTGAGCGTGTAAAGAGAATGCTAGAAACAGTTGATGCCGAAGGTGACACTCCTCTCCATCACGCAGCAAGAGGCGAGCATGCTGATATCGTAAGATTGTTGCTGGCTTCCGGAGCTTCACCTACCAAGGCAAACCTATACGGAAAGATCCCAAGTGACTTACCGGAAGCCGACACAGAAGCTAGGAGAATTTTGGAGGCTGCTTCTGCTGCTACGACGTGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

206

Amino Acids

21.86

Weight (kDa)

4.3

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_4 PF13637 44 - 92 5.4e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 45 - 127 6.5e-16 Ankyrin repeats (3 copies)
Ank_5 PF13857 70 - 109 3.2e-08 Ankyrin repeats (many copies)
Ank PF00023 71 - 102 3e-10 Ankyrin repeat
Ank_3 PF13606 71 - 99 2.7e-06 Ankyrin repeat
Ank_KRIT1 PF24521 72 - 173 3.6e-07 KRIT1 ankyrin-repeats domain
Ank_4 PF13637 79 - 125 2.5e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 129 - 173 4.7e-07 Ankyrin repeats (3 copies)
Ank PF00023 145 - 175 3.7e-08 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014540)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G09890 AT3G09890
fragaria_vesca FvH4_6g16830
malus_domestica MD12G1106800.v1.1
prunus_persica Prupe.7G125700_v2.0.a1
pyrus_communis pycom12g10730
rosa_chinensis RchiOBHm_Chr3g0470841
rosa_laevigata RLG00000024241
rosa_multiflora Rmu_co8290787.1_g000001 Rmu_sc0002790.1_g000024
rosa_roxburghii Rroxscaffold_6G00410490
rosa_rugosa Rorug03G0113100
rosa_samantha Rh3AG162800 Rh3BG187900 Rh3CG177900 Rh3DG187900
rosa_wichuraiana Rw3G015330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 104, 120
AccIII TCCGGA 1 cut(s) 500
AciI CCGC 2 cut(s) 5, 16
AclWI GGATC 1 cut(s) 532
AcsI RAATTY 2 cut(s) 289, 579
AcyI GRCGYC 2 cut(s) 132, 156
AfiI CCNNNNNNNGG 1 cut(s) 18
AgsI TTSAA 1 cut(s) 365
AjiI CACGTC 1 cut(s) 609
AleI CACNNNNGTG 1 cut(s) 356
AluBI AGCT 4 cut(s) 91, 224, 506, 572
AluI AGCT 4 cut(s) 91, 224, 506, 572
Alw26I GTCTC 2 cut(s) 210, 245
AlwI GGATC 1 cut(s) 532
Ama87I CYCGRG 1 cut(s) 11
Aor13HI TCCGGA 1 cut(s) 500
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 4 cut(s) 189, 455, 590, 599
ApoI RAATTY 2 cut(s) 289, 579
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 2 cut(s) 106, 122
AsuC2I CCSGG 2 cut(s) 12, 13
AsuHPI GGTGA 2 cut(s) 446, 501
AvaI CYCGRG 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 315
BbvI GCAGC 4 cut(s) 201, 467, 577, 586
BccI CCATC 5 cut(s) 25, 131, 206, 237, 456
BciVI GTATCC 1 cut(s) 341
BcnI CCSGG 2 cut(s) 12, 13
BcoDI GTCTC 2 cut(s) 210, 245
BfaI CTAG 2 cut(s) 413, 573
BfuI GTATCC 1 cut(s) 341
BisI GCNGC 4 cut(s) 190, 456, 591, 600
BlsI GCNGC 4 cut(s) 191, 457, 592, 601
Bme1390I CCNGG 2 cut(s) 12, 13
BmeT110I CYCGRG 1 cut(s) 11
BmgBI CACGTC 1 cut(s) 609
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 2 cut(s) 12, 13
BmsI GCATC 3 cut(s) 190, 322, 415
BpiI GAAGAC 1 cut(s) 315
Bpu10I CCTNAGC 1 cut(s) 300
BpuMI CCSGG 2 cut(s) 12, 13
BsaHI GRCGYC 2 cut(s) 132, 156
BsaJI CCNNGG 2 cut(s) 11, 516
BsaWI WCCGGW 2 cut(s) 500, 553
BsaXI ACNNNNNCTCC 2 cut(s) 10, 40
Bsc4I CCNNNNNNNGG 1 cut(s) 18
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 2 cut(s) 265, 613
BseAI TCCGGA 1 cut(s) 500
BseDI CCNNGG 2 cut(s) 11, 516
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 1 cut(s) 18
BseMII CTCAG 2 cut(s) 152, 384
BseNI ACTGG 2 cut(s) 265, 613
BseRI GAGGAG 2 cut(s) 101, 432
BseXI GCAGC 4 cut(s) 201, 467, 577, 586
Bsh1236I CGCG 2 cut(s) 104, 120
BshFI GGCC 1 cut(s) 236
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 4 cut(s) 12, 342, 501, 554
BslI CCNNNNNNNGG 1 cut(s) 18
BsmAI GTCTC 2 cut(s) 210, 245
BsmI GAATGC 1 cut(s) 414
BsnI GGCC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 11
Bsp13I TCCGGA 1 cut(s) 500
Bsp143I GATC 1 cut(s) 537
BspACI CCGC 2 cut(s) 5, 16
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 2 cut(s) 151, 385
BspEI TCCGGA 1 cut(s) 500
BspFNI CGCG 2 cut(s) 104, 120
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 1 cut(s) 532
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 2 cut(s) 265, 613
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 2 cut(s) 11, 516
BssMI GATC 1 cut(s) 537
BssNI GRCGYC 2 cut(s) 132, 156
BssT1I CCWWGG 1 cut(s) 516
Bst4CI ACNGT 2 cut(s) 380, 421
Bst6I CTCTTC 2 cut(s) 33, 69
BstACI GRCGYC 2 cut(s) 132, 156
BstC8I GCNNGC 3 cut(s) 467, 471, 495
BstDEI CTNAG 4 cut(s) 138, 181, 300, 393
BstF5I GGATG 1 cut(s) 310
BstFNI CGCG 2 cut(s) 104, 120
BstHHI GCGC 2 cut(s) 106, 122
BstKTI GATC 1 cut(s) 540
BstMAI GTCTC 2 cut(s) 210, 245
BstMBI GATC 1 cut(s) 537
BstMWI GCNNNNNNNGC 3 cut(s) 503, 596, 599
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 10, 11
BstUI CGCG 2 cut(s) 104, 120
BstV1I GCAGC 4 cut(s) 201, 467, 577, 586
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 1 cut(s) 537
BstYI RGATCY 1 cut(s) 537
BsuI GTATCC 1 cut(s) 341
BsuRI GGCC 1 cut(s) 236
BtgZI GCGATG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 609
BtsCI GGATG 1 cut(s) 310
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 2 cut(s) 210, 385
Cac8I GCNNGC 3 cut(s) 467, 471, 495
CfoI GCGC 2 cut(s) 106, 122
Cfr10I RCCGGY 1 cut(s) 341
Cfr9I CCCGGG 1 cut(s) 11
CseI GACGC 3 cut(s) 104, 140, 164
CviAII CATG 2 cut(s) 329, 470
CviJI RGCY 9 cut(s) 91, 224, 236, 299, 497, 506, 560, 572, 590
CviKI_1 RGCY 9 cut(s) 91, 224, 236, 299, 497, 506, 560, 572, 590
DdeI CTNAG 4 cut(s) 138, 181, 300, 393
DpnI GATC 1 cut(s) 539
DpnII GATC 1 cut(s) 537
Eam1104I CTCTTC 2 cut(s) 33, 69
EarI CTCTTC 2 cut(s) 33, 69
EciI GGCGGA 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 516
Eco32I GATATC 1 cut(s) 478
Eco88I CYCGRG 1 cut(s) 11
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 516
FaeI CATG 2 cut(s) 332, 473
FaiI YATR 4 cut(s) 246, 330, 471, 529
FatI CATG 2 cut(s) 328, 469
Fnu4HI GCNGC 4 cut(s) 190, 456, 591, 600
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 4 cut(s) 190, 456, 591, 600
FspBI CTAG 2 cut(s) 413, 573
GlaI GCGC 2 cut(s) 105, 121
GluI GCNGC 4 cut(s) 190, 456, 591, 600
HaeIII GGCC 1 cut(s) 236
HapII CCGG 4 cut(s) 12, 342, 501, 554
HgaI GACGC 3 cut(s) 104, 140, 164
HhaI GCGC 2 cut(s) 106, 122
Hin1I GRCGYC 2 cut(s) 132, 156
Hin1II CATG 2 cut(s) 332, 473
Hin6I GCGC 2 cut(s) 104, 120
HinP1I GCGC 2 cut(s) 104, 120
HinfI GANTC 1 cut(s) 389
HpaII CCGG 4 cut(s) 12, 342, 501, 554
HphI GGTGA 2 cut(s) 446, 501
Hpy188I TCNGA 2 cut(s) 30, 394
Hpy188III TCNNGA 2 cut(s) 173, 501
Hpy99I CGWCG 2 cut(s) 158, 610
HpyAV CCTTC 3 cut(s) 125, 170, 425
HpyCH4III ACNGT 2 cut(s) 380, 421
HpyCH4IV ACGT 1 cut(s) 608
HpyCH4V TGCA 3 cut(s) 203, 287, 328
HpyF10VI GCNNNNNNNGC 3 cut(s) 503, 596, 599
HpyF3I CTNAG 4 cut(s) 138, 181, 300, 393
HpySE526I ACGT 1 cut(s) 608
Hsp92I GRCGYC 2 cut(s) 132, 156
Hsp92II CATG 2 cut(s) 332, 473
HspAI GCGC 2 cut(s) 104, 120
Kpn2I TCCGGA 1 cut(s) 500
Kzo9I GATC 1 cut(s) 537
LmnI GCTCC 2 cut(s) 88, 503
LpnPI CCDG 9 cut(s) 25, 128, 218, 250, 278, 355, 479, 514, 567
Lsp1109I GCAGC 4 cut(s) 201, 467, 577, 586
LweI GCATC 3 cut(s) 190, 322, 415
MaeI CTAG 2 cut(s) 413, 573
MaeII ACGT 1 cut(s) 608
MaeIII GTNAC 3 cut(s) 267, 434, 545
MalI GATC 1 cut(s) 539
MboI GATC 1 cut(s) 537
MboII GAAGA 4 cut(s) 50, 86, 221, 320
MflI RGATCY 1 cut(s) 537
MluCI AATT 4 cut(s) 289, 318, 372, 579
MroI TCCGGA 1 cut(s) 500
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 2 cut(s) 371, 375
MslI CAYNNNNRTG 1 cut(s) 356
MspI CCGG 4 cut(s) 12, 342, 501, 554
MspR9I CCNGG 2 cut(s) 12, 13
Mva1269I GAATGC 1 cut(s) 414
MvnI CGCG 2 cut(s) 104, 120
MwoI GCNNNNNNNGC 3 cut(s) 503, 596, 599
NciI CCSGG 2 cut(s) 12, 13
NdeII GATC 1 cut(s) 537
NlaIII CATG 2 cut(s) 332, 473
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 2 cut(s) 434, 545
NspI RCATGY 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 356
PacI TTAATTAA 1 cut(s) 375
PaeI GCATGC 1 cut(s) 473
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 414
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 1 cut(s) 389
PkrI GCNGC 4 cut(s) 191, 457, 592, 601
PspN4I GGNNCC 1 cut(s) 9
PsuI RGATCY 1 cut(s) 537
RseI CAYNNNNRTG 1 cut(s) 356
SaqAI TTAA 2 cut(s) 371, 375
SatI GCNGC 4 cut(s) 190, 456, 591, 600
Sau3AI GATC 1 cut(s) 537
ScrFI CCNGG 2 cut(s) 12, 13
SfaNI GCATC 3 cut(s) 190, 322, 415
SmaI CCCGGG 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 356
SphI GCATGC 1 cut(s) 473
Sse9I AATT 4 cut(s) 289, 318, 372, 579
SsiI CCGC 2 cut(s) 5, 16
SspMI CTAG 2 cut(s) 413, 573
StyD4I CCNGG 2 cut(s) 10, 11
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 2 cut(s) 380, 421
TaiI ACGT 1 cut(s) 611
TaqI TCGA 2 cut(s) 69, 153
TasI AATT 4 cut(s) 289, 318, 372, 579
TfiI GAWTC 1 cut(s) 389
Tru1I TTAA 2 cut(s) 371, 375
Tru9I TTAA 2 cut(s) 371, 375
TscAI CASTG 2 cut(s) 210, 385
TseFI GTSAC 2 cut(s) 434, 545
TseI GCWGC 4 cut(s) 189, 455, 590, 599
Tsp45I GTSAC 2 cut(s) 434, 545
TspDTI ATGAA 1 cut(s) 321
TspGWI ACGGA 1 cut(s) 546
TspMI CCCGGG 1 cut(s) 11
TspRI CASTG 2 cut(s) 210, 385
XapI RAATTY 2 cut(s) 289, 579
XceI RCATGY 1 cut(s) 473
XmaI CCCGGG 1 cut(s) 11
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 2 cut(s) 413, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.